5,814 results match your criteria American Journal of Respiratory Cell and Molecular Biology[Journal]


Transcriptomic responses to Ivacaftor and prediction of Ivacaftor clinical responsiveness.

Am J Respir Cell Mol Biol 2019 Apr 17. Epub 2019 Apr 17.

University of Pittsburgh, Medicine, Pittsburgh, Pennsylvania, United States ;

Ivacaftor is a recently FDA-approved drug for the treatment of cystic fibrosis (CF) patients with at least one copy of the G511D mutation in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. The transcriptomic effect of Ivacaftor in CF patients remains unclear. We aim to examine if and how the transcriptome of patients is influenced by Ivacaftor treatment and to determine if these data allow prediction of Ivacaftor responsiveness. Read More

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https://www.atsjournals.org/doi/10.1165/rcmb.2019-0032OC
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http://dx.doi.org/10.1165/rcmb.2019-0032OCDOI Listing
April 2019
2 Reads

The Human Lung Cell Atlas - A high-resolution reference map of the human lung in health and disease.

Am J Respir Cell Mol Biol 2019 Apr 17. Epub 2019 Apr 17.

University of Groningen, University Medical Center Groningen, Pathology and Medical Biology, Groningen, Netherlands.

Lung disease accounts for every sixth death globally. Profiling the molecular state of all lung cell types in health and disease is currently revolutionizing the identification of disease mechanisms and will aid the design of novel diagnostic and personalized therapeutic regimens. Recent progress in high-throughput techniques for single-cell genomic and transcriptomic analyses has opened up new possibilities to study individual cells within a tissue, classify these into cell types, and characterize variations in their molecular profiles as a function of genetics, environment, cell-cell interactions, developmental processes, ageing or disease. Read More

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https://www.atsjournals.org/doi/10.1165/rcmb.2018-0416TR
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http://dx.doi.org/10.1165/rcmb.2018-0416TRDOI Listing
April 2019
1 Read
3.985 Impact Factor

Preterm Lung Exhibits Distinct Spatiotemporal Proteome Expression at Initiation of Lung Injury.

Am J Respir Cell Mol Biol 2019 Apr 17. Epub 2019 Apr 17.

Murdoch Childrens Research Institute, 34361, Neonatal Research, Parkville, Victoria, Australia.

To characterize time-dependent and regionally-specific injury patterns associated with early ventilation of the preterm lung using a mass-spectrometry based proteomic approach. Preterm lambs delivered at gestational age 124-127d were randomized to receive 15-min (n=7) or 90-min (n=10) of mechanical ventilation at standardized settings (positive end-expiratory pressure 8 cmHO, tidal volume 6-8 mL/kg) and were compared with unventilated control lambs (n=7). At study completion, lung tissue was taken from standardized gravity-dependent and non-dependent regions, and assessed for lung injury via histology, qPCR and by proteomic analysis via Orbitrap-mass spectrometry. Read More

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https://www.atsjournals.org/doi/10.1165/rcmb.2019-0084OC
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http://dx.doi.org/10.1165/rcmb.2019-0084OCDOI Listing
April 2019
1 Read

CRACking the Beat of Cilia: Calcium Rocks.

Am J Respir Cell Mol Biol 2019 Apr 16. Epub 2019 Apr 16.

Ulm University, Institute of General Physiology, Ulm, Germany ;

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http://dx.doi.org/10.1165/rcmb.2019-0072EDDOI Listing

To Bet or Not To Bet on T-Bet As A Therapeutic Target in Emphysema?

Am J Respir Cell Mol Biol 2019 Apr 15. Epub 2019 Apr 15.

Brigham and Women's Hospital, 1861, Boston, Massachusetts, United States.

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http://dx.doi.org/10.1165/rcmb.2019-0118EDDOI Listing
April 2019
2 Reads

CD44 and xCT: The Silver Bullet for Endothelial-to-Mesenchymal Transition in Pulmonary Arterial Hypertension?

Am J Respir Cell Mol Biol 2019 Apr 15. Epub 2019 Apr 15.

Vanderbilt University School of Medicine, Nashville, Tennessee, United States ;

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http://dx.doi.org/10.1165/rcmb.2019-0135EDDOI Listing

Beyond Fibroblast Heterogeneity: What Single-Cell RNA-Seq Tells Us.

Authors:
Yong Zhou

Am J Respir Cell Mol Biol 2019 Apr 12. Epub 2019 Apr 12.

University of Alabama at Birmingham, Birmingham, Alabama, United States ;

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http://dx.doi.org/10.1165/rcmb.2019-0120EDDOI Listing
April 2019
3.985 Impact Factor

Organophosphorus Pesticides Induce Cytokine Release from Differentiated Human THP1 Cells.

Am J Respir Cell Mol Biol 2019 Apr 12. Epub 2019 Apr 12.

Oregon Health & Science University, Pulmonary and Critical Care Medicine, Portland, Oregon, United States.

Epidemiologic studies link organophosphorus pesticides (OPs) to increased incidence of asthma. In guinea pigs, OP-induced airway hyperreactivity requires macrophages and TNFα. Here, we determine whether OPs interact directly with macrophages to alter cytokine expression or release. Read More

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https://www.atsjournals.org/doi/10.1165/rcmb.2018-0257OC
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http://dx.doi.org/10.1165/rcmb.2018-0257OCDOI Listing
April 2019
1 Read

Smoking Impairs the Immunomodulatory Capacity of Lung Resident Mesenchymal Stem Cells in COPD.

Am J Respir Cell Mol Biol 2019 Apr 12. Epub 2019 Apr 12.

Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Madrid, Spain ;

Tobacco smoking is the main environmental risk factor of Chronic Obstructive Pulmonary Disease (COPD), but not all smokers develop the disease. A population of lung resident mesenchymal stem cells (LR-MSC) exist in healthy lungs, but how tobacco smoking affects them and their role in COPD have not been assessed yet. Using a sphere based culture technique, LR-MSC were isolated from lung tissue obtained from non-smokers, current and former smokers with and without COPD (n=53). Read More

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https://www.atsjournals.org/doi/10.1165/rcmb.2018-0351OC
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http://dx.doi.org/10.1165/rcmb.2018-0351OCDOI Listing
April 2019
2 Reads

Cigarette Smoke Triggers IL-33-Associated Inflammation in a Model of Late Stage COPD.

Am J Respir Cell Mol Biol 2019 Apr 11. Epub 2019 Apr 11.

University of California, San Diego, Medicine/ Physiology, La Jolla, California, United States ;

Chronic Obstructive Pulmonary Disease (COPD) is a worldwide threat. Cigarette smoke (CS) exposure causes cardiopulmonary disease, COPD and increases the risk for pulmonary tumors. In addition to poor lung function, those with COPD are susceptible to bouts of dangerous inflammation triggered by pollutants or infection. Read More

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http://dx.doi.org/10.1165/rcmb.2018-0402OCDOI Listing
April 2019
1 Read

CA Dreamin': Carbonic Anhydrase Inhibitors, Macrophages and Pulmonary Hypertension.

Am J Respir Cell Mol Biol 2019 Apr 11. Epub 2019 Apr 11.

Johns Hopkins, Baltimore, Maryland, United States ;

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http://dx.doi.org/10.1165/rcmb.2019-0122EDDOI Listing

Glutamine Metabolism is Required for Collagen Protein Synthesis in Lung Fibroblasts.

Am J Respir Cell Mol Biol 2019 Apr 11. Epub 2019 Apr 11.

University of Chicago, 2462, Chicago, Illinois, United States ;

Idiopathic pulmonary fibrosis is characterized by the TGF-β-dependent differentiation of lung fibroblasts into myofibroblasts, leading to excessive deposition of extracellular matrix proteins, which distort lung architecture and function. Metabolic reprogramming in myofibroblasts is emerging as an important mechanism in the pathogenesis IPF and recent evidence suggests that glutamine metabolism is required in myofibroblasts although the exact role of glutamine in myofibroblasts is unclear. Here we demonstrate that glutamine, and its conversion to glutamate by glutaminase is required for TGF-β-induced collagen protein production in lung fibroblasts. Read More

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https://www.atsjournals.org/doi/10.1165/rcmb.2019-0008OC
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April 2019
6 Reads

Microbial Metabolites in Cystic Fibrosis: a Target for Future Therapy?

Am J Respir Cell Mol Biol 2019 Apr 9. Epub 2019 Apr 9.

Medical University of Vienna, 27271, Department of Medicine 1, Wien, Wien, Austria ;

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http://dx.doi.org/10.1165/rcmb.2019-0081EDDOI Listing
April 2019
1 Read

Transcription Factor T-Bet Attenuates the Development of Elastase-Induced Emphysema in Mice.

Am J Respir Cell Mol Biol 2019 Apr 9. Epub 2019 Apr 9.

University of Tsukuba, 13121, Respiratory Medicine, Tsukuba, Japan.

Chronic obstructive pulmonary disease (COPD) is a progressive lung disease characterized by peripheral airways inflammation and emphysema. Emerging evidence indicates a contribution of both innate and adaptive immune cells to the development of COPD. Transcription factor T-bet modulates the function of immune cells and therefore might be involved in the pathogenesis of COPD. Read More

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https://www.atsjournals.org/doi/10.1165/rcmb.2018-0109OC
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http://dx.doi.org/10.1165/rcmb.2018-0109OCDOI Listing
April 2019
3 Reads

Cellular Senescence: The Trojan Horse in Chronic Lung Diseases.

Am J Respir Cell Mol Biol 2019 Apr 9. Epub 2019 Apr 9.

University of Pittsburgh, Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, Pittsburgh, Pennsylvania, United States ;

Senescence is a cell-fate decision characterized by irreversible arrest of proliferation accompanied by senescence associated secretory phenotype (SASP). Traditionally, cellular senescence was recognized as a beneficial physiological mechanism during development, wound healing, and in tumor suppression. However, in recent years identification of negative consequences of cellular senescence has emerged, illuminating its role in several chronic pathologies. Read More

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http://dx.doi.org/10.1165/rcmb.2018-0410TRDOI Listing

Gamma-Delta T Cells in Lung Cancer Malignant Pleural Effusion. Friend? Foe?

Authors:
Edmund K Moon

Am J Respir Cell Mol Biol 2019 Apr 8. Epub 2019 Apr 8.

University of Pennsylvania, 6572, Pulmonary and Critical Care, Philadelphia, Pennsylvania, United States ;

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http://dx.doi.org/10.1165/rcmb.2019-0080EDDOI Listing
April 2019
1 Read

Proline-Glycine-Proline Peptides Are Critical in the Development of Smoke-Induced Emphysema.

Am J Respir Cell Mol Biol 2019 Apr 8. Epub 2019 Apr 8.

University of Alabama at Birmingham, 9968, Birmingham, Alabama, United States ;

Chronic obstructive pulmonary disease (COPD) is a major cause of worldwide mortality and is characterized by an excessive airway neutrophilic response. The neutrophil chemoattractant proline-glycine-proline (PGP) and its more potent acetylated form (acPGP) have been found to be elevated in COPD patients and act via CXCR2. Here, we investigate the impact of neutralizing PGP peptides in a murine model for emphysema. Read More

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http://dx.doi.org/10.1165/rcmb.2018-0216OCDOI Listing
April 2019
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Role of Differential Estrogen Receptor Activation on Airway Hyperreactivity and Remodeling in a Murine Model of Asthma.

Am J Respir Cell Mol Biol 2019 Apr 8. Epub 2019 Apr 8.

North Dakota State University, Pharmaceutical Sciences, School of Pharmacy, College of Health Professions, Fargo, North Dakota, United States.

Evidence suggest that airway hyperresponsiveness (AHR) is a characteristic feature of asthma. Epidemiological studies confirm severity of asthma is greater in women, suggesting a critical role of female sex steroid hormones (especially estrogen). Also, very few in vivo studies report role of sex steroid hormones in asthma; however, sequence of events through differential activation of estrogen receptors (ERs) remain unanswered in asthmatic airways. Read More

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http://dx.doi.org/10.1165/rcmb.2018-0321OCDOI Listing
April 2019
1 Read

A Need for Targeted Immunosuppression Following Lung Transplantation.

Authors:
Ankit Bharat

Am J Respir Cell Mol Biol 2019 Apr 8. Epub 2019 Apr 8.

Northwestern University, Chicago, Illinois, United States ;

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http://dx.doi.org/10.1165/rcmb.2019-0100EDDOI Listing
April 2019
1 Read

Carbonic Anhydrase Inhibition Ameliorates Inflammation and Experimental Pulmonary Hypertension.

Am J Respir Cell Mol Biol 2019 Apr 5. Epub 2019 Apr 5.

Brigham and Women's Hospital, Pediatric Newborn Medicine, Boston, Massachusetts, United States.

Rationale: Inflammation and vascular smooth muscle cell (VSMC) phenotypic switching are causally linked to pulmonary arterial hypertension (PAH) pathogenesis. Carbonic anhydrase inhibition (CAI) induces mild metabolic acidosis and exerts protective effects in hypoxic pulmonary hypertension (PH). Carbonic anhydrases and metabolic acidosis are further known to modulate immune cell activation. Read More

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http://dx.doi.org/10.1165/rcmb.2018-0232OCDOI Listing
April 2019
2 Reads

Cystic Fibrosis Inflammation: Hyper-inflammatory, Hypo-inflammatory, or Both?

Am J Respir Cell Mol Biol 2019 Apr 5. Epub 2019 Apr 5.

Univ. North Carolina Chapel Hill, Medicine, Marsico Lung Institute, Cystic Fibrosis Center, Chapel Hill, North Carolina, United States.

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http://dx.doi.org/10.1165/rcmb.2019-0107EDDOI Listing
April 2019
1 Read

Frequenting Sequencing: How Genetics Teaches us Cilia Biology.

Am J Respir Cell Mol Biol 2019 Apr 5. Epub 2019 Apr 5.

Washington University in Saint Louis, 7548, Medicine, Saint Louis, Missouri, United States ;

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http://dx.doi.org/10.1165/rcmb.2019-0103EDDOI Listing

Orchestrating Airway Smooth Muscle Cell Migration: GMFγ Phosphorylation is the Key.

Am J Respir Cell Mol Biol 2019 Apr 5. Epub 2019 Apr 5.

Duke University, Medicine, Durham, North Carolina, United States ;

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http://dx.doi.org/10.1165/rcmb.2019-0074EDDOI Listing

Bronchial Epithelial Calcium Metabolism Impairment in Smokers and COPD: Decreased ORAI3 Signaling.

Am J Respir Cell Mol Biol 2019 Apr 3. Epub 2019 Apr 3.

CHRU Montpellier, Respiratory Disease Department, Montpellier, France.

The airway epithelium represents a fragile environmental interface potentially disturbed by cigarette smoke, the major risk factor for developing Chronic Obstructive Pulmonary Disease (COPD). Cigarette smoke leads to bronchial epithelial damage on ciliated, goblet and club cells, which could involve calcium signaling. Calcium is a key messenger involved in virtually all fundamental physiological functions, including mucus and cytokine secretion, cilia beating and epithelial repair. Read More

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http://dx.doi.org/10.1165/rcmb.2018-0228OCDOI Listing
April 2019
3 Reads

The Innate Immune Protein S100A9 Protects from Th2-Mediated Allergic Airway Inflammation.

Am J Respir Cell Mol Biol 2019 Apr 3. Epub 2019 Apr 3.

Vanderbilt University Medical Center, 12328, Pathology, Microbiology, and Immunology, Nashville, Tennessee, United States.

Calprotectin is a heterodimer of the proteins S100A8 and S100A9 and is an abundant innate immune protein associated with inflammation. In humans, calprotectin transcription and protein abundance are associated with asthma and disease severity. However, mechanistic studies in experimental asthma models have been inconclusive, identifying both protective and pathogenic effects of calprotectin. Read More

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http://dx.doi.org/10.1165/rcmb.2018-0217OCDOI Listing
April 2019
2 Reads

Inhibition of Glutaminase 1 Attenuates Experimental Pulmonary Fibrosis.

Am J Respir Cell Mol Biol 2019 Apr 3. Epub 2019 Apr 3.

University of Alabama at Birmingham, Birmingham, Alabama, United States ;

It has been increasingly recognized lately that aberrant cellular metabolism plays an important role in the pathogenesis of pulmonary fibrosis. In our previous systemic studies, we found that human lung myofibroblasts undergo glutaminolytic reprogramming, which is mediated by an increased expression of glutaminase 1 (Gls1). We showed that augmented glutaminolysis critically regulates collagen production by promoting its stabilization in human lung myofibroblasts. Read More

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https://www.atsjournals.org/doi/10.1165/rcmb.2019-0051OC
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http://dx.doi.org/10.1165/rcmb.2019-0051OCDOI Listing
April 2019
4 Reads

April Highlights/Papers by Junior Investigators/NIH News.

Authors:

Am J Respir Cell Mol Biol 2019 Apr;60(4):iv

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http://dx.doi.org/10.1165/rcmb.604ivDOI Listing

The Kinome of Human Alveolar Type II and Basal Cells, and its Reprogramming in Lung Cancer.

Am J Respir Cell Mol Biol 2019 Mar 27. Epub 2019 Mar 27.

National Jewish Health, Medicine, Denver, Colorado, United States.

The discovery of mutant tyrosine kinases as oncogenic drivers of lung adenocarcinomas has changed our basic understanding of the development and therapy of lung cancer. Yet, we have an incomplete understanding of the expressed kinases (kinome) in lung cancer progenitor cells and whether kinase expression and the overall kinome changes, or is reprogrammed, during transformation. We performed RNA-sequencing on freshly isolated human Alveolar Type II cells (ATII), Basal Cells (BC), and lung cancer cell lines to define the normal kinome in non-transformed cells and identify kinome changes during transformation. Read More

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http://dx.doi.org/10.1165/rcmb.2018-0283OCDOI Listing

CDHR3 Asthma-Risk Genotype Affects Susceptibility of Airway Epithelium to Rhinovirus C Infections.

Am J Respir Cell Mol Biol 2019 Mar 27. Epub 2019 Mar 27.

University of Wisconsin-Madison, Pediatrics, Madison, Wisconsin, United States ;

Cadherin-related family member 3 (CDHR3) is a transmembrane protein that is highly expressed in airway epithelia and the only known receptor for rhinovirus C (RV-C). A CDHR3 SNP (rs6967330) with 'G' to 'A' base change, has been linked to severe exacerbations of asthma and increased susceptibility to RV-C infections in young children. The goals of this study were to determine the subcellular localization of CDHR3 and to test the hypothesis that CDHR3 asthma-risk genotype affects epithelial cell function and susceptibility to RV-C infections of the airway epithelia. Read More

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http://dx.doi.org/10.1165/rcmb.2018-0220OCDOI Listing

CFAP300: Mutations in Slavic Primary Ciliary Dyskinesia Patients and a Role in Ciliary Dynein Arms Trafficking.

Am J Respir Cell Mol Biol 2019 Mar 27. Epub 2019 Mar 27.

Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland.

Primary ciliary dyskinesia (PCD) is a rare, genetically heterogeneous hereditary disease from a class of ciliopathies. In spite of the recent progress, the genetic basis of PCD in 1/3 of patients remains unknown. In search for new genes and/or mutations, the whole-exome sequencing was performed in 120 unrelated Polish PCD patients, in whom no genetic cause of PCD was earlier identified. Read More

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http://dx.doi.org/10.1165/rcmb.2018-0260OCDOI Listing
March 2019
7 Reads

Three is Better than One: An Improved Multiple Hit Model of Primary Graft Dysfunction.

Am J Respir Cell Mol Biol 2019 Mar 25. Epub 2019 Mar 25.

University of Colorado Denver School of Medicine, 12225, Medicine, Aurora, Colorado, United States ;

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http://dx.doi.org/10.1165/rcmb.2019-0082EDDOI Listing

Glucocorticoids and Airway Smooth Muscle: A Few More Answers, Still More Questions.

Authors:
Ajay P Nayak

Am J Respir Cell Mol Biol 2019 Mar 25. Epub 2019 Mar 25.

Thomas Jefferson University, 6559, Medicine, Philadelphia, Pennsylvania, United States ;

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http://dx.doi.org/10.1165/rcmb.2019-0089EDDOI Listing

CircRNA-012091/PPP1R13B-Mediated Lung Fibrotic Response in Silicosis via ER Stress and Autophagy.

Am J Respir Cell Mol Biol 2019 Mar 25. Epub 2019 Mar 25.

Southeast University, Department of physiology, Nanjing, Jiangsu, China ;

Silicosis is a progressive fibrotic disease of lung tissue caused by long-term inhalation of SiO2. However, relatively few studies on the direct effects of SiO2 on lung fibroblasts have been performed. PPP1R13B is a major member of the apoptosis-stimulating proteins of the p53 family (ASPPs), but its role in pulmonary fibrosis is unclear. Read More

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http://dx.doi.org/10.1165/rcmb.2019-0017OCDOI Listing
March 2019
1 Read
3.985 Impact Factor

Caspase-1 Inhibition Attenuates Hyperoxia-Induced Lung and Brain Injury in Neonatal Mice.

Am J Respir Cell Mol Biol 2019 Mar 21. Epub 2019 Mar 21.

University of Miami School of Medicine, Pediatrics/Neonatology, Miami, United States ;

Hyperoxia plays a key role in the development of bronchopulmonary dysplasia (BPD), a chronic lung disease of preterm infants. Infants with BPD often have brain injury that leads to long-term neurodevelopmental impairment, but the underlying mechanisms controlling BPD-induced neurodevelopmental impairment remain unclear. Our previous studies have shown that hyperoxia-induced BPD in rodents is associated with lung inflammasome activation. Read More

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http://dx.doi.org/10.1165/rcmb.2018-0192OCDOI Listing
March 2019
2 Reads

Endothelial-Mesenchymal Transition Drives Expression of CD44 Variant and xCT in Pulmonary Hypertension.

Am J Respir Cell Mol Biol 2019 Mar 21. Epub 2019 Mar 21.

Keio University School of Medicine, Department of Cardiology, Tokyo, Japan ;

Pulmonary arterial hypertension (PAH) pathogenesis shares similarities with carcinogenesis. One CD44 variant (CD44v) isoform, CD44v8-10, binds to and stabilizes the cystine transporter subunit (xCT), producing reduced glutathione (GSH) and thereby enhancing the antioxidant defense of cancer stem cells. Pharmacological inhibition of xCT by sulfasalazine suppresses tumor growth, survival, and resistance to chemotherapy. Read More

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http://dx.doi.org/10.1165/rcmb.2018-0231OCDOI Listing
March 2019
6 Reads

Syndecan-2: Old Player in a New Field.

Am J Respir Cell Mol Biol 2019 Mar 21. Epub 2019 Mar 21.

Univ. of Pennsylvania, Medicine, Philadelphia, Pennsylvania, United States ;

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https://www.atsjournals.org/doi/10.1165/rcmb.2019-0033ED
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http://dx.doi.org/10.1165/rcmb.2019-0033EDDOI Listing
March 2019
5 Reads

Mice with a Deletion of Rsph1 Exhibit a Low Level of Mucociliary Clearance and Develop a PCD Phenotype.

Am J Respir Cell Mol Biol 2019 Mar 21. Epub 2019 Mar 21.

University of North Carolina at Chapel Hill, Marsico Lung Institute/Cystic Fibrosis Center, Chapel Hill, North Carolina, United States ;

Primary ciliary dyskinesia (PCD) is a genetically and phenotypically heterogeneous disease caused by mutations in over 40 different genes. Individuals with PCD caused by mutations in radial spoke head 1 homolog (RSPH1) have been reported to have a milder phenotype compared to other PCD subjects, as evidenced by a lower incidence of neonatal respiratory distress, higher nasal nitric oxide levels, and better lung function. To better understand genotype-phenotype relationships in PCD, we have characterized a mutant mouse model with a deletion of Rsph1. Read More

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http://dx.doi.org/10.1165/rcmb.2017-0387OCDOI Listing
March 2019
2 Reads

Lessons Learned from the ABCs of Granuloma Formation.

Am J Respir Cell Mol Biol 2019 Mar 18. Epub 2019 Mar 18.

The Ohio State University, Department of Internal Medicine, Division of Pulmonary, Critical Care and Sleep Medicine, Columbus, Ohio, United States ;

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http://dx.doi.org/10.1165/rcmb.2019-0076EDDOI Listing

Omics and the Search for Blood Biomarkers in COPD: Insights from COPDGene.

Am J Respir Cell Mol Biol 2019 Mar 15. Epub 2019 Mar 15.

National Jewish Health, 2930, Department of Medicine, Denver, Colorado, United States.

Background: There is an unmet need for blood biomarkers in diagnosis and prognosis of chronic obstructive pulmonary disease (COPD). The search for these biomarkers has been revolutionized by high throughput sequencing techniques and multiplex platforms which can measure thousands of gene transcripts, proteins, or metabolites. We review COPDGene publications which include DNA methylation, transcriptomics, proteomics, and metabolomic blood biomarkers and discuss their impact on COPD. Read More

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http://dx.doi.org/10.1165/rcmb.2018-0245PSDOI Listing
March 2019
1 Read

Proteostasis Takes Center Stage in Pulmonary Fibrosis.

Am J Respir Cell Mol Biol 2019 Mar 8. Epub 2019 Mar 8.

Northwestern University, Pulmonary and Critical Care Medicine, Chicago, Illinois, United States ;

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http://dx.doi.org/10.1165/rcmb.2018-0400EDDOI Listing

Trametinib Attenuates Delayed Rejection and Preserves Thymic Function in Rat Lung Transplantation.

Am J Respir Cell Mol Biol 2019 Mar 8. Epub 2019 Mar 8.

Kyoto University Graduate School of Medicine, Thoracic Surgery, Kyoto, Japan.

Rationale: Delayed immunological rejection following human lung transplantation causes chronic lung allograft dysfunction (CLAD), which is associated with high mortality. Delayed rejection may be attributable to indirect alloantigen presentation by host antigen-presenting cells (APCs); however, its pathophysiology is not fully understood. The mitogen-activated protein kinase pathway is activated in T cells upon stimulation, and we previously showed that the MEK inhibitor trametinib suppresses graft-versus-host disease following murine bone marrow transplantation. Read More

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https://www.atsjournals.org/doi/10.1165/rcmb.2018-0188OC
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http://dx.doi.org/10.1165/rcmb.2018-0188OCDOI Listing
March 2019
5 Reads

Platelets: Lone Rangers of Inflammatory Signaling in the Lung.

Am J Respir Cell Mol Biol 2019 Mar 8. Epub 2019 Mar 8.

Univ of Pittsburgh, Pittsburgh, United States ;

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http://dx.doi.org/10.1165/rcmb.2019-0057EDDOI Listing
March 2019
1 Read

Defining the Activated Fibroblast Population in Lung Fibrosis Using Single Cell Sequencing.

Am J Respir Cell Mol Biol 2019 Mar 8. Epub 2019 Mar 8.

Regeneron Pharmaceuticals Inc, 7845, Cardiovascular, Renal, and Fibrosis Research, Tarrytown, New York, United States ;

Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal lung disorder driven by unrelenting extracellular matrix (ECM) deposition. Fibroblasts are recognized as the central mediators of ECM production in IPF, however, the characteristics of the underlying fibroblast cell populations in IPF remain poorly understood. Here we use an unbiased single cell RNA-sequencing analysis of a bleomycin-induced pulmonary fibrosis model to characterize molecular responses to fibrotic injury. Read More

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http://dx.doi.org/10.1165/rcmb.2018-0313OCDOI Listing
March 2019
1 Read
3.985 Impact Factor

Cystic Fibrosis Plasma Blunts the Immune Response to Bacterial Infection.

Am J Respir Cell Mol Biol 2019 Mar 8. Epub 2019 Mar 8.

Ann and Robert H Lurie Children's Hospital of Chicago, 2429, Chicago, Illinois, United States.

Rationale: Cystic fibrosis (CF) is caused by mutations of the gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR). It remains unclear whether the abnormal immune response in CF involves extrinsic signals released from the external or internal environment.

Objectives: To characterize the peripheral immune signatures in CF and its association with clinical phenotypes. Read More

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http://dx.doi.org/10.1165/rcmb.2018-0114OCDOI Listing
March 2019
4 Reads

Alveolar Macrophage ABCG1 Deficiency Promotes Pulmonary Granulomatous Inflammation.

Am J Respir Cell Mol Biol 2019 Mar 8. Epub 2019 Mar 8.

East Carolina University, Dept of Internal Medicine, Greenville, North Carolina, United States ;

Pulmonary granuloma formation is a complex and poorly understood response to inhaled pathogens and particulate matter. To explore the mechanisms of pulmonary granuloma formation and maintenance our laboratory has developed a multi-wall carbon nanotube (MWCNT) induced murine model of chronic granulomatous inflammation. We have demonstrated that the MWCNT model closely mimics pulmonary sarcoidosis pathophysiology, including the deficiency of alveolar macrophage ATP-binding cassette (ABC) lipid transporters ABCA1 and ABCG1. Read More

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http://dx.doi.org/10.1165/rcmb.2018-0365OCDOI Listing
March 2019
3 Reads

SMAD Signaling Restricts Mucous Cell Differentiation In Human Airway Epithelium.

Am J Respir Cell Mol Biol 2019 Mar 8. Epub 2019 Mar 8.

Massachusetts General Hospital, The Mucosal Immunology & Biology Research Center , Boston, Massachusetts, United States.

Mucin-secreting goblet cell metaplasia and hyperplasia (GCMH) is a common pathological phenotype in many human respiratory diseases including asthma, chronic obstructive pulmonary disease, cystic fibrosis, primary ciliary dyskinesia, and infections. A better understanding of how goblet cell quantities or proportions in the airway epithelium are regulated may provide novel therapeutic targets to mitigate GCMH in these devastating diseases. We identify canonical SMAD signaling as the principle pathway restricting goblet cell differentiation in human airway epithelium. Read More

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http://dx.doi.org/10.1165/rcmb.2018-0326OCDOI Listing
March 2019
1 Read

Nonsense Mediated RNA Decay Pathway Inhibition Restores Expression and Function of W1282X CFTR.

Am J Respir Cell Mol Biol 2019 Mar 5. Epub 2019 Mar 5.

Ionis Pharmaceuticals, Inc., Antisense Drug Discovery, Carlsbad, California, United States ;

Rationale: The recessive genetic disease cystic fibrosis is caused by loss of function mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Approximately 10% of cystic fibrosis patients have at least one allele with a nonsense mutation in CFTR. Nonsense mutations generate premature termination codons that can subject mRNA transcripts to rapid degradation through the nonsense mediated mRNA decay (NMD) pathway. Read More

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http://dx.doi.org/10.1165/rcmb.2018-0316OCDOI Listing
March 2019
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March Highlights/Papers by Junior Investigators/NIH News.

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Am J Respir Cell Mol Biol 2019 Mar;60(3):iii

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http://dx.doi.org/10.1165/rcmb.603iiiDOI Listing

miR-144 Mediated Inhibition of ROCK1 Protects Against LPS Induced Lung Endothelial Hyperpermeability.

Am J Respir Cell Mol Biol 2019 Feb 27. Epub 2019 Feb 27.

Ann and Robert H Lurie Children's Hospital of Chicago, 2429, Chicago, Illinois, United States ;

Dysfunctional endothelial cell (EC) barrier and increased lung vascular permeability is a cardinal feature of Acute Lung Injury (ALI) and sepsis that may results in a pathophysiological condition characterized by alveolar flooding, pulmonary edema and subsequent hypoxemia. In lung ECs, activation of Rho associated kinase-1 (ROCK1) phosphorylates myosin light chain (MLC) associated phosphatase at its inhibitory site which favors phosphorylation of MLC, stress fiber formation and hyper-permeability during ALI. The role of MicroRNA-144 (miR-144) has been well investigated in many human diseas¬es including cardiac ischaemia/reperfusion-induced injury, lung cancer and lung viral infection, however, its role in pulmonary EC barrier regulation remains obscure. Read More

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http://dx.doi.org/10.1165/rcmb.2018-0235OCDOI Listing
February 2019