3,447 results match your criteria American Journal of Reproductive Immunology [Journal]


Are B Cells Altered in the Decidua of Women with Preterm or Term Labor?

Am J Reprod Immunol 2019 Feb 15:e13102. Epub 2019 Feb 15.

Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services, Bethesda, Maryland, and Detroit, Michigan, USA.

Problem: The immunophenotype of B cells at the maternal-fetal interface (decidua) in labor at term and preterm labor is poorly understood.

Method Of Study: Decidual tissues were obtained from women with preterm or term labor and from non-labor gestational age-matched controls. Immunophenotyping of decidual B cells was performed using multi-color flow cytometry. Read More

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February 2019

Toll-Like Receptor 9, maternal cell-free dna and myometrial cell response to cpg oligodeoxynucleotide stimulation.

Am J Reprod Immunol 2019 Feb 13:e13100. Epub 2019 Feb 13.

Center for Perinatal Research, The Research Institute at Nationwide Children's Hospital, Columbus, OH, 43215.

Problem: Among mechanisms triggering onset of parturition, it has been recently postulated that Toll-Like Receptor (TLR)9 engagement by cell-free DNA (cfDNA) triggers inflammation, myometrial contractions and labor in absence of infection. The current study evaluated whether direct (myometrial) or indirect (decidual) TLR9 engagement enhances human myometrial contractility.

Method Of Study: TLR9 expression and cellular localization were surveyed by immunohistochemistry of placenta, fetal membranes, and myometrium in term (gestational age [GA]:>37 weeks) labor (TL, n=7) or term non-labor (TNL, n=7) tissues. Read More

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February 2019

CD49a regulates the function of human decidual natural killer cells.

Am J Reprod Immunol 2019 Feb 13:e13101. Epub 2019 Feb 13.

Department of Pathophysiology, Anhui Medical University, 81 Mei-Shan Road, Hefei, Anhui Province, 230032, China.

Problem: The function of CD49a on human decidual natural killer (dNK) cells is unknown.

Method Of Study: The expression of CD49a on dNK cells from human patients with recurrent spontaneous abortions or age-matched healthy controls was analyzed by flow cytomertry. DNK cells were treated with CD49a neutralizing antibody and analyzed for function (cytokines production and cytotoxic activity). Read More

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February 2019
6 Reads

Myeloid Derived Suppressor Cells Depletion May Cause Pregnancy Loss via Upregulating the Cytotoxicity of Decidual Natural Killer Cells.

Am J Reprod Immunol 2019 Feb 9:e13099. Epub 2019 Feb 9.

International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Problem: Maternal immune system tolerance to the semiallogeneic fetus is critical for a successful pregnancy. Studies have shown that myeloid-derived suppressor cells (MDSCs) play an important role in maintaining feto-maternal tolerance. However, the mechanisms remain poorly understood. Read More

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February 2019
2 Reads

The immunophenotype of decidual macrophages in acute atherosis.

Am J Reprod Immunol 2019 Feb 8:e13098. Epub 2019 Feb 8.

Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U. S. Department of Health and Human Services Bethesda, Maryland, and Detroit, Michigan, USA.

Problem: Acute atherosis is a uteroplacental arterial lesion that is associated with pregnancy complications such as preeclampsia and preterm birth, the leading cause of perinatal morbidity and mortality worldwide. However, the immunobiology of acute atherosis is poorly understood.

Method Of Study: Placental basal plate samples were collected from women who delivered with (n = 11) and without (n = 31) decidua basalis lesions of acute atherosis. Read More

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February 2019
8 Reads

The role of monocytic myeloid-derived suppressor cells in the pathogenesis of endometriosis.

Am J Reprod Immunol 2019 Jan 28:e13094. Epub 2019 Jan 28.

Academic Unit of Obstetrics and Gynecology, IRCCS Ospedale Policlinico San Martino, Genoa, Italy.

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January 2019
4 Reads

Tacrolimus treatment for infertility related to maternal-fetal immune interactions.

Authors:
Koushi Yamaguchi

Am J Reprod Immunol 2019 Jan 28:e13097. Epub 2019 Jan 28.

Center of Maternal-Fetal, Neonatal and Reproductive Medicine, National Center for Child Health and Development, Tokyo, Japan.

Many approaches have been used to achieve successful pregnancies in patients with infertility, though existing treatments remain unsatisfactory in patients with infertility caused by abnormal maternal-fetal immunity. However, our understanding of the immunological aspects of infertility has steadily progressed, aided by recent research into organ transplantation and cancer. The results of these recent analyses have led to the development and evaluation of several candidate immunological treatments, but the use of immunological treatments remains a novel approach. Read More

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January 2019
8 Reads

Evidence for an association between endometriosis, fibromyalgia and autoimmune diseases.

Am J Reprod Immunol 2019 Jan 25:e13095. Epub 2019 Jan 25.

Maccabi Institute for Research and Innovation, Maccabi Healthcare Services, Tel Aviv, Israel.

Problem: The aim of this study was to investigate the prevalence of and the association between endometriosis, fibromyalgia and autoimmune disease (AID) in a large population database and to describe healthcare resource utilization (HCRU) in affected women.

Methods: A retrospective cross-sectional study was performed using the computerized databases of Maccabi Healthcare Services (MHS), a 2.1-million-member healthcare plan in Israel. Read More

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January 2019
1 Read

Nisin reduces uterine inflammation in rats by modulating the concentrations of the pro- and anti-inflammatory cytokines.

Am J Reprod Immunol 2019 Jan 25:e13096. Epub 2019 Jan 25.

College of Animal Science, Inner Mongolia Agricultural University, Hohhot, 010018, P. R. China.

Problem: The Staphylococcus aureus has been found to be associated with clinical endometritis of cow. The result of oral antibiotic remains poor. Therefore, this study investigates the role of nisin in endometritis. Read More

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January 2019
1 Read

Alteration of the immune cell profiles in the pathophysiology of tubal ectopic pregnancy.

Am J Reprod Immunol 2019 Jan 23:e13093. Epub 2019 Jan 23.

Department of Obstetrics& Gynaecology, The University of Hong Kong, Hong Kong.

Tubal ectopic pregnancy (TEP) refers to implantation of conceptus in the fallopian tube. It makes up over 98% of ectopic pregnancy (EP), which is the leading cause of maternal morbidity and mortality in the first trimester of pregnancy. Immune cells at the maternal-fetal interface play important roles in the process of embryo implantation, stroma decidualization, and early placental development. Read More

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January 2019
1 Read

Lectin pathway proteins of the complement system in normotensive pregnancy and preeclampsia.

Am J Reprod Immunol 2019 Jan 23:e13092. Epub 2019 Jan 23.

Department of Clinical Medicine, Aarhus University, Denmark.

Problem: The lectin pathway of the complement system may be involved in the pathogenesis of preeclampsia. We aimed to investigate changes in serum concentrations of a broad range of lectin pathway proteins during normal pregnancy and their association with preeclampsia, placental infarctions and intrauterine growth restriction (IUGR).

Method Of Study: We included 51 women with normotensive pregnancies and 54 women with pregnancies complicated by preeclampsia. Read More

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January 2019
3 Reads

Combining individual Chlamydia trachomatis IgG antibodies MOMP, TARP, CPAF, OMP2, and HSP60 for tubal factor infertility prediction.

Am J Reprod Immunol 2019 Jan 10:e13091. Epub 2019 Jan 10.

Department of Medical Microbiology & Infection Control, Laboratory of Immunogenetics, Amsterdam UMC, VU University Medical Centre, Amsterdam, The Netherlands.

Problem: Tubal factor infertility (TFI) is a severe complication of genital Chlamydia trachomatis infections. In fertility workup, chlamydia antibody test (CAT) is used to predict TFI. The predictive value for TFI of most commonly used CAT is moderate. Read More

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January 2019
1 Read

Serum markers of B-cell activation in pregnancy during late gestation, delivery, and the postpartum period.

Am J Reprod Immunol 2019 Jan 9:e13090. Epub 2019 Jan 9.

Centre for Rheumatology and Bloomsbury Rheumatology Unit, Division of Medicine, University College London, London, UK.

Problem: B cells are vital for the normal evolution of pregnancy due to their humoral and possible regulatory activities. Our group and others have documented that circulating B-cell subsets undergo changes from normal late pregnancy to the postpartum period. However, the underlying mechanisms are poorly understood. Read More

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January 2019
1 Read

The underdeveloped innate immunity in embryonic stem cells: The molecular basis and biological perspectives from early embryogenesis.

Authors:
Yan-Lin Guo

Am J Reprod Immunol 2019 Jan 4:e13089. Epub 2019 Jan 4.

Department of Cell and Molecular Biology, University of Southern Mississippi, Hattiesburg, Mississippi.

Embryonic stem cells (ESCs) have been intensively studied as a promising cell source for regenerative medicine. The rapid advancements in the field have not only proven the feasibility of ESC-based cell therapy, but also led to a better understanding of pluripotent stem cells (PSCs) as a unique cell population at an early stage of embryogenesis. Recent studies have revealed that both human and mouse ESCs have attenuated innate immune responses to infectious agents and inflammatory cytokines. Read More

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January 2019
2 Reads

Insights of Efferocytosis in Normal and Pathological Pregnancy.

Am J Reprod Immunol 2019 Jan 4:e13088. Epub 2019 Jan 4.

Laboratory for Reproductive Immunology, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, 200011, People's Republic of China.

Efferocytosis, which is known as the phagocytic clearance of dying cells by professional as well as non-professional phagocytes, including a great number of intracellular/extracellular factors and signals, is interrelated with the immune system, contributing to local and systemic homeostasis, especially in tissues with high constitutive rates of apoptosis. Accumulating studies have indicated that immune dysregulation is associated with the pathogenesis of the female reproductive system, which causes Pre-eclampsia(PE), Recurrent Spontaneous Abortion (RSA), ruptured ectopic pregnancy, and so on. And some studies have revealed the pleiotropic and essential role of efferocytosis in these obstetrical disorders. Read More

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January 2019
2 Reads

Metabolic syndrome mediates proinflammatory responses of inflammatory cells in preeclampsia.

Am J Reprod Immunol 2019 Jan 5:e13086. Epub 2019 Jan 5.

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Problem: To investigate whether metabolic syndrome (MetS) is associated with exacerbation of inflammatory responses in preeclamptic (PE) patients, the dynamic changes of Th17 and Treg cells, monocytes, cytokines, and transcription pattern of inflammasome-related genes were analyzed in 35 women with PE suffering from MetS in comparison to 38 PE women without MetS and healthy pregnant women.

Method Of Study: Expression of inflammasome-related genes, cytokines, and also TLR4 was measured using real-time PCR. Serum and medium supernatant cytokines levels of PBMCs and serum levels of HMGB1 and Caspase-1 were also evaluated by ELISA. Read More

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January 2019
2 Reads

Association of raised levels of IL-4 and anti-TPO with hyperprolactinemia.

Am J Reprod Immunol 2019 Jan 4:e13085. Epub 2019 Jan 4.

Department of Obstetrics and Gynecology, All India Institute of Medical Sciences, New Delhi, India.

Background And Objective: The modulatory role of prolactin in autoimmune regulation is well established. Hyperprolactinemia is often associated with autoimmune disease like systemic lupus erythematosus and autoimmune thyroid diseases. The objective was to compare levels of direct and indirect autoimmune factors in different categories of hyperprolactinemia cases and predict the direction of association between hyperprolactinemia and autoimmune factors, if any. Read More

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January 2019
3 Reads

Early changes of the heat-shock protein 60 to 70 ratio as prediction of miscarriage in pregnancy.

Am J Reprod Immunol 2019 Jan 4:e13087. Epub 2019 Jan 4.

4th Department of Internal Medicine, Medical School, National and Kapodistrian University of Athens, Athens, Greece.

Problem: To study the balance of circulating heat shock protein (HSP)60 and HSP70 in pre-term delivery.

Method Of Study: A two-stage approach was used. At first stage, we run retrospective analysis of prospective collected clinical data, and at a second stage, we studied an animal model of pre-term delivery (PTD). Read More

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January 2019
3 Reads

Progesterone modulates the T-cell response via glucocorticoid receptor-dependent pathways.

Am J Reprod Immunol 2019 Jan 2:e13084. Epub 2019 Jan 2.

Department of Obstetrics and Fetal Medicine, Laboratory for Experimental, Feto-Maternal Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Problem: Steroid hormones such as progesterone and glucocorticoids rise during pregnancy and are accountable for the adaptation of the maternal immune system to pregnancy. How steroid hormones induce fetal tolerance is not fully understood. We hypothesized that steroid hormones selectively regulate the T-cell response by promoting T-cell death. Read More

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January 2019
1 Read

New models of lipopolysaccharide-induced implantation loss reveal insights into the inflammatory response.

Am J Reprod Immunol 2019 Jan 2:e13082. Epub 2019 Jan 2.

Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale School of Medicine, New Haven, Connecticut.

Problem: Chronic endometritis, inflammation of the uterizzvvne lining caused by common gram-negative bacterial strains or mycoplasma, has been associated with unexplained implantation failure and infertility. However, limited models of bacteria-induced implantation loss exist to study the molecular changes that occur in vivo. The goal of this study was to provide a new resource to study the process of bacteria-induced inflammation and implantation loss utilizing common experimental models: C57Bl/6 mice and primary human endometrial stromal cells. Read More

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January 2019
5 Reads

Chronic hepatitis B infection alters peripheral immune response in women with reproductive failure.

Am J Reprod Immunol 2019 Jan 2:e13083. Epub 2019 Jan 2.

Shenzhen Key Laboratory of Reproductive Immunology for Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, Guangdong, China.

Problem: Does hepatitis B infection affect peripheral blood immune response in women with reproductive failure?

Method Of Study: Two hundred and twenty-seven women, including 10 HBsAg HBeAg , 27 HBsAg HBeAg hepatitis sero-positive women, and 190 women without HBV infection, formed the study population. Their peripheral immune responses containing lymphocyte subsets, cytokine production, expression of cell surface markers and intracellular toxicity molecules, and pregnancy outcomes were retrospectively compared.

Results: Comparing with HBsAg HBeAg carriers and HBsAg group, HBsAg HBeAg group had lower rates of CD3 CD4 helper T cells (31. Read More

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January 2019
3 Reads

Intrauterine infusion of human chorionic gonadotropin before embryo transfer in IVF/ET cycle: The critical review.

Am J Reprod Immunol 2018 Dec 27:e13077. Epub 2018 Dec 27.

Department of Microbiology and Immunology, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, Illinois.

Intrauterine infusion of human chorionic gonadotropin (IUI-hCG) has been proposed to improve the outcome of in vitro fertilization-embryo transfer (IVF-ET), since it plays a critical role in synchronizing endometrial and fetal development. As the early mediator from embryo, hCG promotes the decidualization, angiogenesis, maternal immune tolerance, and trophoblast invasion, favoring successful implantation of embryo. Although multiple clinical trials have been conducted to verify the efficacy of IUI-hCG on IVF-ET outcome in recent years, the findings remained controversial. Read More

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December 2018
1 Read

Altered crosstalk of estradiol and progesterone with Myeloid-derived suppressor cells and Th1/Th2 cytokines in early miscarriage is associated with early breakdown of maternal-fetal tolerance.

Am J Reprod Immunol 2018 Dec 27:e13081. Epub 2018 Dec 27.

Department of Molecular & Human Genetics, Banaras Hindu University, Varanasi, India.

Problem: Decline in myeloid-derived suppressor cells (MDSCs) and Th2 cytokines levels lead to early miscarriage (EM) but how the hormonal milieu of the body regulates MDSCs and Th1/Th2 cytokine balance is still a matter of investigation.

Method Of Study: Peripheral blood and decidua samples were collected from 20 EM patients, and 20 healthy pregnant women opted for elective abortion. MDSCs and G-MDSCs levels were analyzed in peripheral blood mononuclear cells, and Th1/Th2 cytokines levels were determined in serum via flow cytometry. Read More

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December 2018
15 Reads
2.438 Impact Factor

GATA6 expression promoted by an active enhancer may become a molecular marker in endometriosis lesions.

Am J Reprod Immunol 2018 Dec 27:e13078. Epub 2018 Dec 27.

Department of Obstetrics and Gynecology, Tottori University Faculty of Medicine, Yonago, Japan.

Problem: Genome-wide profiling of DNA methylation in endometriotic cells has shown a distinct facet of epigenetic backgrounds; however, specific DNA methylation sites responsible for aberrant gene expression in endometriosis were unknown. Are there specific endometriosis-associated DNA methylations that can be used as molecular markers in endometriosis lesions?

Method Of Study: This study used endometriotic tissues from the chocolate cyst lining of the ovaries of patients with endometriosis, and endometrial tissues from disease-free patients. For analysis, stromal cells were collected from endometrial and endometriotic tissues. Read More

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December 2018
2 Reads

Repeated lipopolysaccharide exposure leads to placental endotoxin tolerance.

Am J Reprod Immunol 2018 Dec 26:e13080. Epub 2018 Dec 26.

Women and Children's Research Laboratory, NYU Winthrop Hospital, Mineola, New York.

Problem: Placental infection induces increased levels of pro-inflammatory cytokines, which have been implicated in the pathogenesis of pre-term labor. Endotoxin tolerance is a phenomenon in which exposure to a dose of endotoxin makes tissue less responsive to subsequent exposures. The objective of our study was to determine whether repeated exposure to endotoxin will induce a tolerant phenotype in normal human second-trimester placental tissue. Read More

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December 2018
4 Reads

Protein kinase D mediates inflammatory responses of human placental macrophages to Group B Streptococcus.

Am J Reprod Immunol 2018 Dec 24:e13075. Epub 2018 Dec 24.

Department of Microbiology and Immunology, Meharry Medical College School of Medicine, Nashville, Tennessee.

Problem: During pregnancy, Group B Streptococcus (GBS) can infect fetal membranes to cause chorioamnionitis, resulting in adverse pregnancy outcomes. Macrophages are the primary resident phagocyte in extraplacental membranes. Protein kinase D (PKD) was recently implicated in mediating pro-inflammatory macrophage responses to GBS outside of the reproductive system. Read More

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December 2018
2 Reads

Novel 3D in vitro models to evaluate trophoblast migration and invasion.

Am J Reprod Immunol 2018 Dec 24:e13076. Epub 2018 Dec 24.

Department of Obstetrics, Gynecology and Reproductive Sciences, Division of Reproductive Sciences, Yale School of Medicine, New Haven, Connecticut.

Problem: Embryo implantation depends on the interactions between the developing embryo and the maternal endometrium. Signals originating from the decidua play a critical role in the process of implantation and trophoblast invasion; however, the molecular mechanisms mediating this interaction are poorly understood. The objective of this study was to develop in vitro models that would mimic the processes of attachment, migration, and early invasion of the trophoblast. Read More

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December 2018
2 Reads

Identification of programmed cell death 1 and its ligand in the testicular tissue of mice.

Am J Reprod Immunol 2018 Dec 22:e13079. Epub 2018 Dec 22.

Family Planning Research Institute, Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Problem: This study aims to determine the expression and localization of programmed cell death 1 (PD-1) and programmed cell death 1 ligand 1 (PD-L1) in the testes of mice at different developmental stages.

Method Of Study: By means of RT-qPCR, Western blot and immunofluorescence, the expression and localization of PD-1 and PD-L1 were detected in the testicular tissues of mice at different postnatal times: P7, P14, P21, P28, P35, and adulthood. Meanwhile, the level of soluble PD-L1 (sPD-L1) was evaluated by ELISA in the testicular interstitial fluid (IF) of the adult mice, culture supernatants of TM4 cell lines (Sertoli cells lines), and primary Sertoli cells at P14. Read More

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December 2018
7 Reads

Long non-coding RNA AFAP1-AS1 promoting epithelial-mesenchymal transition of endometriosis is correlated with transcription factor ZEB1.

Am J Reprod Immunol 2019 Jan 19;81(1):e13074. Epub 2018 Dec 19.

First Affiliated Hospital of Xiamen University, Xiamen, China.

Problem: The role of the long non-coding RNA (lncRNA) actin filament associated protein 1 antisense RNA1 (AFAP1-AS1) in the etiology of endometriosis is unknown.

Method Of Study: Expression of epithelial-mesenchymal transition (EMT) markers was quantified using qRT-PCR, immunohistochemistry, and Western blotting. The proliferation, migration, and invasion of ectopic endometrial epithelial cells and Ishikawa cells were evaluated by MTT, EdU, wound healing, and transwell assays. Read More

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January 2019
9 Reads

Cervico-vaginal secretion cytokine profile: A non-invasive approach to study the endometrial receptivity in IVF cycles.

Am J Reprod Immunol 2019 Jan 26;81(1):e13064. Epub 2018 Nov 26.

Institute for Maternal and Child Health, IRCCS "Burlo Garofolo", Trieste, Italy.

Problem: Cytokines have a significant role in the process of embryo implantation, trophoblast growth, and differentiation by modulating the immune and endocrine system. The aim of this study was to investigate the profile of a large set of cytokines in the cervico-vaginal washing of women undergoing IVF, to explore the association of these proteins with a good receptive endometrium.

Method Of Study: A cohort of 155 women scheduled for IVF cycle was recruited. Read More

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January 2019
14 Reads
2.438 Impact Factor

Extracellular matrix remodeling effects of serum amyloid A1 in the human amnion: Implications for fetal membrane rupture.

Am J Reprod Immunol 2019 Jan 10;81(1):e13073. Epub 2018 Dec 10.

Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, China.

Problem: Rupture of fetal membranes is a crucial event at parturition, which is preceded by extensive extracellular matrix (ECM) remodeling. Our recent studies have demonstrated that the human fetal membranes are capable of de novo synthesis of serum amyloid A1 (SAA1), an acute phase protein, and the abundance of SAA1 in the amnion was increased at parturition. However, the exact role of SAA1 in human parturition remains to be established. Read More

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January 2019
10 Reads

Adverse pregnancy and perinatal outcome in patients with recurrent pregnancy loss: Multiple imputation analyses with propensity score adjustment applied to a large-scale birth cohort of the Japan Environment and Children's Study.

Am J Reprod Immunol 2019 Jan 13;81(1):e13072. Epub 2018 Dec 13.

Department of Pediatrics and Neonatology, Nagoya City University, Graduate School of Medical Sciences, Nagoya, Japan.

Problem: Several studies have reported the increased risk of preterm birth, premature rupture of membranes, and low birth weight in patients with recurrent pregnancy loss (RPL). There have been a limited number of large population-based studies examining adverse pregnancy and perinatal outcome after RPL. Multiple-imputed analyses (MIA) adjusting for biases due to missing data is also lacking. Read More

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January 2019
21 Reads

Sex-specific influence of the vacuolar adenosine triphosphatase a2 isoform on outcome in twin pregnancies.

Am J Reprod Immunol 2019 Jan 26;81(1):e13071. Epub 2018 Nov 26.

Department of Obstetrics and Gynecology, Weill Cornell Medicine, New York, New York.

Problem: The influence of fetal sex on immune responses in multifetal pregnancies remains incompletely elucidated. The a2 isoform of vacuolar adenosine triphosphatase (a2V) is expressed on the cell membrane of maternal lymphoid cells and contributes to down-regulation of pro-inflammatory immune responses during gestation. The association between fetal sex and a2V expression on peripheral blood mononuclear cells (PBMCs) from mothers with twin gestations was assessed. Read More

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January 2019
11 Reads

The analysis of human leukocyte antigen-G level in patients with endometrial cancer by Western blot technique.

Am J Reprod Immunol 2019 Jan 29;81(1):e13070. Epub 2018 Nov 29.

Jagiellonian University Medical College, Kraków, Poland.

Problem: Aberrant expression of human leukocyte antigen-G (HLA-G) in various malignancies has been shown to participate in tumour development by suppressing immune regulation within the tumour microenvironment. The detection of HLA-G has reportedly been correlated with certain clinicopathological parameters in several neoplasms. Both the soluble and membranous forms of HLA-G are biologically active, and therefore, we aimed to evaluate the HLA-G level by Western blot technique. Read More

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January 2019
13 Reads

Type I interferons differentially modulate maternal host immunity to infection by Listeria monocytogenes and Salmonella enterica serovar Typhimurium during pregnancy.

Am J Reprod Immunol 2019 Jan 4;81(1):e13068. Epub 2018 Dec 4.

Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario, Canada.

Problem: IFN-alpha receptor deficiency (IFNAR ) enhances immunity to Listeria monocytogenes (LM) and Salmonella enterica serovar Typhimurium (ST) in the non-pregnant state by inhibiting pathogen-induced immune cell death. However, the roles of IFNAR signaling in modulating immunity to infection during pregnancy are not well understood.

Method Of Study: C57BL/6J wild-type (WT) and IFNAR mice were infected systemically with LM or ST. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344237PMC
January 2019
4 Reads

Day-7 embryos generate an anti-inflammatory immune response in peripheral blood immune cells in superovulated cows.

Am J Reprod Immunol 2018 Oct 30:e13069. Epub 2018 Oct 30.

Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.

Problem: Recently, we demonstrated both in vitro and in vivo that an immunological crosstalk between Day-7 embryo and immune cells exists locally in the uterus in cows. The peripheral immune response to early embryos at Day-7 of pregnancy in cows remains largely unknown. Thus, we aimed to investigate the response of peripheral blood immune cells in the presence of multiple Day-7 embryos in the uterus in donor cows of embryo transfer program. Read More

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October 2018
11 Reads

Endometrial microbes and microbiome: Recent insights on the inflammatory and immune "players" of the human endometrium.

Am J Reprod Immunol 2018 Dec 30;80(6):e13065. Epub 2018 Oct 30.

Dipartimento di Scienze della Salute della Donna e del Bambino, Area Salute Donna, Fondazione Policlinico Universitario A.Gemelli IRCCS, Rome, Italia.

In recent years, extended scientific works shed light on the important role played by the endometrium in early pregnancy. This review examines our current knowledge about the delicate balance between microbial and cellular immune agents at endometrial level: All of them might affect endometrial receptivity. In contrast to the classical thinking of human endometrium as a sterile tissue, several recent studies have drawn attention to a resident population of microorganisms, which reaches only a 30% of concordance with those of the cervical-vaginal flora. Read More

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http://doi.wiley.com/10.1111/aji.13065
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December 2018
6 Reads

CD33 CD14 CD11b HLA-DR monocytic myeloid-derived suppressor cells recruited and activated by CCR9/CCL25 are crucial for the pathogenic progression of endometriosis.

Am J Reprod Immunol 2019 Jan 16;81(1):e13067. Epub 2018 Nov 16.

Department of Obstetrics & Gynecology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Problem: Endometriosis (EM) is a chronic immunoinflammatory disease associated with an abnormal immunotolerant microenvironment. Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of immature myeloid cells that play a major role in immunosuppression in cancer, inflammation and other diseases. This paper aims to elucidate whether or not MDSCs are involved in regulating this microenvironment in EM and how this regulation occurs. Read More

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January 2019
20 Reads

Genome-wide identification of microRNAs in decidual natural killer cells from patients with unexplained recurrent spontaneous abortion.

Am J Reprod Immunol 2018 Nov 19;80(5):e13052. Epub 2018 Oct 19.

Department of Obstetrics and Gynecology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, Henan, China.

Problem: This study revealed miRNA regulation and functional microarray-based profiles of miRNAs in the natural killer (NK) cells of the decidual tissue obtained from patients with unexplained recurrent spontaneous abortion (URSA).

Method Of Study: Patients with URSA were categorized based on the occurrence of at least two or more successive spontaneous abortions between 7th and 10th gestational week. Total RNA was isolated from the NK cells of the decidual tissue obtained from patients with induced abortion at about the 8th gestational week. Read More

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November 2018
12 Reads

Autophagy gene ATG5 knockdown upregulates apoptotic cell death during Candida albicans infection in human vaginal epithelial cells.

Am J Reprod Immunol 2018 Dec 10;80(6):e13056. Epub 2018 Oct 10.

Division of Molecular Immunology and Microbiology (MIM), National Institute for Research in Reproductive Health (NIRRH), Mumbai, India.

Problem: Candida albicans infection induces damage in host cells that results in the activation of cell death pathways such as apoptosis and necrosis. Autophagy acts as a pro-survival mechanism during various infections and has cross talks with apoptosis. The objective of our study was to delineate the effect of autophagy knockdown in human vaginal epithelial cells (VECs) during Candida infection-induced apoptosis. Read More

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http://doi.wiley.com/10.1111/aji.13056
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December 2018
2 Reads

Plasmacytoma variant translocation 1 (PVT1) regulates trophoblast viability, proliferation, and migration and is downregulated in spontaneous abortion.

Am J Reprod Immunol 2019 Jan 17;81(1):e13048. Epub 2018 Sep 17.

Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, China.

Problem: Human pregnancy is a complex biological process, and spontaneous abortion is the most common complication of pregnancy. LncRNAs have been identified that play key roles in a variety of human diseases. Recently, lncRNAPVT1 was reported to relate to the pathogenesis and progression of pregnancy. Read More

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http://doi.wiley.com/10.1111/aji.13048
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January 2019
20 Reads

Associations of maternal immune response with MeHg exposure at 28 weeks' gestation in the Seychelles Child Development Study.

Am J Reprod Immunol 2018 Nov 17;80(5):e13046. Epub 2018 Sep 17.

Nutrition Innovation Centre for Food and Health (NICHE), School of Biomedical Sciences, University of Ulster, Coleraine, Northern Ireland.

Problem: Maternal methylmercury (MeHg) exposure may be associated with immune response during pregnancy.

Method Of Study: In the high fish-eating Seychelles Child Development Study Nutrition Cohort 2, we examined the association between maternal MeHg, polyunsaturated fatty acids (PUFA), and immune markers (Th1:Th2; TNF-α, IL-1β, IFN-γ, IL-2, IL-4, IL-5, IL-10, MCP-1, TARC, sFlt-1, VEGF-D, CRP and IL-6) at 28 weeks' gestation. Linear regression examined associations between MeHg exposure and immune markers with and without adjustment for PUFA. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202202PMC
November 2018
4 Reads

Estradiol-regulated innate antiviral responses of human endometrial stromal fibroblasts.

Am J Reprod Immunol 2018 Nov 17;80(5):e13042. Epub 2018 Sep 17.

Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire.

Problem: The contribution of fibroblasts to innate immune protection of the human female reproductive tract (FRT) against viral pathogens is relatively unknown.

Method Of Study: Endometrial (EM), endocervical (Cx) and ectocervical (ECx) fibroblasts were isolated from hysterectomy patients and grown in vitro. Fibroblasts were treated with the viral mimic poly (I:C) in the presence or absence of the sex hormone estradiol (E ), with gene expression measured by real-time RT-PCR and protein secretion by ELISA. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275105PMC
November 2018
1 Read

The advantages of proteomic investigation in the management of male accessory gland infection: A response to Grande et al.

Am J Reprod Immunol 2018 Dec 7;80(6):e13063. Epub 2018 Oct 7.

Department of Clinical and Experimental Medicine, University of Catania, Catania, Italy.

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http://doi.wiley.com/10.1111/aji.13063
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December 2018
3 Reads
2.440 Impact Factor

Vitamin D level affects IVF outcome partially mediated via Th/Tc cell ratio.

Am J Reprod Immunol 2018 Dec 6;80(6):e13050. Epub 2018 Oct 6.

Center for Reproductive Medicine, Anhui Provincial Hospital affiliated with Anhui Medical University, Hefei, China.

Problem: The role of vitamin D (VD) in IVF outcome and immune parameters has not been elucidated well.

Method Of Study: Women undergoing IVF treatment with GnRH agonist (Agonist) and progestin-primed ovarian stimulation (PPOS) protocols were divided into VD lower (VDL, 25(OH)VD ≤20 ng/mL) and VD higher (VDH, 25(OH)VD >20 ng/mL) groups. Follicular fluid (FF) VD level, IVF outcomes, and peripheral blood immunophenotypes by flow cytometry were analyzed. Read More

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December 2018
5 Reads
2.440 Impact Factor

Predicting tubal factor infertility by using markers of humoral and cell-mediated immune response against Chlamydia trachomatis.

Am J Reprod Immunol 2018 Nov 3;80(5):e13051. Epub 2018 Oct 3.

The Department of Obstetrics and Gynecology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.

Problem: The accuracy of Chlamydia trachomatis antibody test in predicting tubal factor infertility (TFI) is limited, and more accurate methods are needed. Cell-mediated immune response (CMI) is crucial in the resolution of pathogen, but it may play an important role in the pathogenesis of C trachomatis-associated tubal damage. We studied whether combining the markers of C trachomatis-induced CMI to humoral immune response improves the accuracy of serology in TFI prediction. Read More

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November 2018
2 Reads

Decidual leucocytes infiltrating human spiral arterioles are rich source of matrix metalloproteinases and degrade extracellular matrix in vitro and in situ.

Am J Reprod Immunol 2019 Jan 29;81(1):e13054. Epub 2018 Sep 29.

Maternal and Fetal Health Research Centre, Institute of Human Development, The University of Manchester, Manchester, UK.

Problem: During pregnancy, the decidual spiral arterioles (SpAs) that supply maternal blood to the placenta undergo a series of changes to optimise the transfer of nutrients and oxygen to the developing foetus. Recent studies have shown that initiation of SpA transformation coincides with decidual leucocyte infiltration. Leucocytes are known to be a source of matrix metalloproteinases (MMPs); however, the complete profile of MMPs expressed by decidual NK cells (dNK) and macrophages has not been characterised. Read More

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January 2019
1 Read

The role of interleukins in preeclampsia: A comprehensive review.

Am J Reprod Immunol 2018 Dec 28;80(6):e13055. Epub 2018 Sep 28.

Assisted Reproduction Unit, Third Department of Obstetrics and Gynecology, "Attikon Hospital", Medical School, National and Kapodistrian University of Athens, Athens, Greece.

Preeclampsia is a multi-system hypertensive disorder of pregnancy, with significant rates of maternal and neonatal morbidity. It represents a major cause of preterm birth, as definitive treatment demands fetal delivery. Although its pathophysiology is complicated, placental hypoxia and endothelial dysfunction constitute established pathogenetic steps of the disease. Read More

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December 2018
6 Reads

Peroxisome proliferator-activated receptor gamma ligands affect NF-kB and cytokine synthesis in the porcine endometrium-An in vitro study.

Am J Reprod Immunol 2019 Jan 28;81(1):e13053. Epub 2018 Sep 28.

Department of Animal Anatomy and Physiology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.

Problem: Cytokines, mediators of the immune response, are involved in the regulation of female reproductive processes during the estrous cycle and pregnancy. The present study aimed to investigate the effect of selected peroxisome proliferator-activated receptor gamma (PPARγ) ligands on the expression of nuclear factor kappa B (NF-κB) and selected cytokines, such as interleukin (IL)-1β, -4, -6, -8, -10, and the leukemia inhibitory factor, in the porcine endometrium on days 10-12 and 14-16 of the estrous cycle (mid- and late luteal phase, respectively) or pregnancy (maternal recognition of pregnancy and beginning of implantation, respectively).

Method Of Study: Endometrial slices were incubated in vitro in the presence of PPARγ agonists, 15-deoxy-Δ12, 14-prostaglandin J or rosiglitazone, and PPARγ antagonist T0070907. Read More

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January 2019
1 Read

Inflammasome activation during spontaneous preterm labor with intra-amniotic infection or sterile intra-amniotic inflammation.

Am J Reprod Immunol 2018 Nov 18;80(5):e13049. Epub 2018 Sep 18.

Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, Michigan.

Problem: The inflammasome is implicated in the mechanisms that lead to spontaneous preterm labor (PTL). However, whether there is inflammasome activation in the amniotic cavity of women with PTL and intra-amniotic infection (IAI) or sterile intra-amniotic inflammation (SIAI) is unknown.

Method Of Study: Amniotic fluid samples were collected from women with PTL who delivered at term (n = 31) or preterm without IAI or SIAI (n = 35), with SIAI (n = 27), or with IAI (n = 17). Read More

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November 2018
16 Reads
2.440 Impact Factor