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    3266 results match your criteria American Journal of Reproductive Immunology [Journal]

    1 OF 66

    The role of immunological testing and intervention in reproductive medicine: A fertile collaboration?
    Am J Reprod Immunol 2017 Nov 20. Epub 2017 Nov 20.
    Department of Clinical Immunology, Sir Charles Gairdner Hospital, Perth, WA, Australia.
    Advances in reproductive medicine have significantly increased the success of fertility treatments. Nevertheless, some women experience recurrent implantation failure (RIF) after in-vitro fertilization (IVF) or recurrent pregnancy loss (RPL). Imbalances in the immune system and failure to achieve immune tolerance to the foetus have been implicated as potentially modifiable causes of idiopathic RIF and RPL. Read More

    The role of indoleamine-2,3-dioxygenase in normal and pathological pregnancies.
    Am J Reprod Immunol 2017 Nov 20. Epub 2017 Nov 20.
    Laboratory for Reproductive Immunology, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, China.
    The survival of allogeneic fetus during pregnancy contradicts the laws of immune responses. Behind this paradoxical phenomenon, the mechanism is quite complex. Indoleamine-2,3-dioxygenase (IDO) is the first and rate-limiting enzyme of tryptophan catabolism. Read More

    Chemokine (C-C motif) ligand 25 expressed by trophoblast cells and leukocytes bearing its receptor Ccr9: An alliance during embryo implantation?
    Am J Reprod Immunol 2017 Nov 20. Epub 2017 Nov 20.
    Department of Cellular and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
    Problem: We hypothesized that trophoblast expression of Ccl25 attracts a specific leukocyte cell population to the implantation site for local regulation.

    Method Of Study: Mice blastocysts, ectoplacental cones, and decidua at gestational days 3.5-7. Read More

    Rapid and simple detection of Ureaplasma species from vaginal swab samples using a loop-mediated isothermal amplification method.
    Am J Reprod Immunol 2017 Nov 20. Epub 2017 Nov 20.
    Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan.
    Problem: Ureaplasma species occasionally cause chorioamnionitis and premature labor. We developed a novel assay employing a loop-mediated isothermal amplification (LAMP) method to detect Ureaplasma parvum and Ureaplasma urealyticum.

    Method Of Study: Loop-mediated isothermal amplification primers were designed to amplify Ureaplasma-specific ureaseB genes. Read More

    Chronic endometritis in patients with unexplained infertility: Prevalence and effects of antibiotic treatment on spontaneous conception.
    Am J Reprod Immunol 2017 Nov 14. Epub 2017 Nov 14.
    Institute of Pathology, Department of Biomedical and Human Oncological Science (DIMO), University of Bari, Bari, Italy.
    Problem: The correlations between chronic endometritis and unexplained infertility are unexplored.

    Method Of Study: We performed a retrospective study on consecutive patients referred to our hysteroscopy service due to unexplained infertility. All women underwent endometrial sampling with histological and cultural examinations. Read More

    Low molecular weight heparin and aspirin exacerbate human endometrial endothelial cell responses to antiphospholipid antibodies.
    Am J Reprod Immunol 2017 Nov 14. Epub 2017 Nov 14.
    Department of Obstetrics, Gynecology & Reproductive Sciences, Yale University, New Haven, CT, USA.
    Problem: Women with antiphospholipid antibodies (aPL) are at risk for pregnancy complications despite treatment with low molecular weight heparin (LMWH) or aspirin (ASA). aPL recognizing beta2 glycoprotein I can target the uterine endothelium, however, little is known about its response to aPL. This study characterized the effect of aPL on human endometrial endothelial cells (HEECs), and the influence of LMWH and ASA. Read More

    Genetic and epigenetic regulation of major histocompatibility complex class I gene expression in bovine trophoblast cells.
    Am J Reprod Immunol 2017 Nov 12. Epub 2017 Nov 12.
    Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT, USA.
    Problem: The regulatory mechanisms governing differential expression of classical major histocompatibility complex (MHC) class I (MHC-Ia) and non-classical MHC class I (MHC-Ib) genes are poorly understood.

    Method Of Study: Quantitative reverse transcription- polymerase chain reaction (PCR) was used to compare the abundance of MHC-I transcripts and related transcription factors in peripheral blood mononuclear cells (PBMC) and placental trophoblast cells (PTC). Methylation of MHC-I CpG islands was detected by bisulfite treatment and next-generation sequencing. Read More

    Medroxyprogesterone acetate-treated human, primary endometrial epithelial cells reveal unique gene expression signature linked to innate immunity and HIV-1 susceptibility.
    Am J Reprod Immunol 2017 Nov 6. Epub 2017 Nov 6.
    Department of Pathology and Molecular Medicine, Michael G. DeGroote Center for Learning and Discovery, McMaster University, Hamilton, ON, Canada.
    Problem: Medroxyprogesterone acetate (MPA), a progestin-based hormonal contraceptive designed to mimic progesterone, has been linked to increased human immunodeficiency virus (HIV-1) susceptibility. Genital epithelial cells (GECs) form the mucosal lining of the female genital tract (FGT) and provide the first line of protection against HIV-1. The impact of endogenous sex hormones or MPA on the gene expression profile of GECs has not been comprehensively documented. Read More

    Inhibition of IAP (inhibitor of apoptosis) proteins represses inflammatory status via nuclear factor-kappa B pathway in murine endometriosis lesions.
    Am J Reprod Immunol 2017 Nov 6. Epub 2017 Nov 6.
    Department of Obstetrics and Gynecology, Research Center for Bioscience and Technology, Tottori University Faculty of Medicine, Yonago, Japan.
    Problem: How is the role of inhibitor of apoptosis proteins (IAPs) in the development of murine endometriosis lesions?

    Method Of Study: BALB/c female mice (n = 36) were used for the murine endometriosis model. Endometriotic lesions were surgically induced in mice by transplanting mouse uterine tissue. After 4 weeks of IAP antagonist (BV6) treatment, the expression of inflammatory factors in the implants was evaluated using real-time RT-PCR. Read More

    Upregulation of Tim-3 expression at feto-maternal interface may explain embryo survival in the CBAxDBA/2 model of abortion.
    Am J Reprod Immunol 2017 Oct 30. Epub 2017 Oct 30.
    Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
    Problem: To understand the mechanisms of action of Tim-3 at the maternal-fetal interface and explore how Tim-3 might be involved in the pathogenesis of abortion by constructing an in vitro trophoblast-lymphocyte system.

    Methods Of Study: Female CBA/J × male DBA/2 matings were used as the abortion-prone model and CBA/J × male BALB/c matings as control. The expression of Tim-3 at the maternal-fetal interface and in the peripheral blood lymphocytes was measured by immunohistochemistry and Western blotting. Read More

    Breastfeeding and autoimmunity: Programing health from the beginning.
    Am J Reprod Immunol 2017 Oct 30. Epub 2017 Oct 30.
    Zabludowicz Center for Autoimmune Diseases, Sheba Medical Center, Tel-Hashomer, Israel.
    Breast milk is not only a completely adapted nutrition source for the newborn but also an impressive array of immune-active molecules that afford protection against infections and shape mucosal immune responses. Decisive imprinting events might be modulated during the first months of life with potential health long-term effects, enhancing the importance of breastfeeding as a major influence on the immune system correct development and modifying disease susceptibility. The aim of this review was to clarify the link between breastfeeding and autoimmune diseases, inquiring the related mechanisms, based on data available in the literature. Read More

    Gestational tissue inflammatory biomarkers at term labor: A systematic review of literature.
    Am J Reprod Immunol 2017 Oct 27. Epub 2017 Oct 27.
    Department of Obstetrics & Gynecology, Division of Maternal-Fetal Medicine & Perinatal Research, The University of Texas Medical Branch at Galveston, Galveston, TX, USA.
    Parturition at term is characterized by inflammatory overload in both feto-maternal tissues. Despite the large number of individual studies on changes in inflammatory biomarkers linked to labor, a comprehensive profile of them in each of the uterine compartments is not available to better understand their mechanistic contributions to labor. This systematic review investigated the pro- and anti-inflammatory biomarkers reported in intra-uterine tissues (amnion, chorion, decidua, placenta, and myometrium) at term labor. Read More

    The immune profile induced is crucial to determine the effects of immunocastration over gonadal function, fertility, and GnRH-I expression.
    Am J Reprod Immunol 2017 Oct 19. Epub 2017 Oct 19.
    Laboratory of Veterinary Vaccines, Department of Animal Biology, Faculty of Veterinary and Animal Science, Universidad de Chile, Santiago, Chile.
    Problem: Immunocastration or vaccination against the GnRH-I hormone is a promising alternative to reproductive control in different animal species. Given the low immunogenicity of this hormone, the use of adjuvants becomes necessary.

    Method Of Study: This study evaluated the effects of three adjuvants that induce different immune response profiles over gonadal function, fertility, and expression of GnRH-I. Read More

    Computational flow cytometry analysis reveals a unique immune signature of the human maternal-fetal interface.
    Am J Reprod Immunol 2017 Oct 14. Epub 2017 Oct 14.
    Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA.
    Problem: Decidual immune dysregulation is thought to underlie major pregnancy disorders; however, incomplete understanding of the decidual immune interface has hampered the mechanistic investigation.

    Method Of Study: Human term decidua was collected, and single-cell phenotypic information was acquired by highly polychromatic flow cytometry. Cellular identity analysis was performed with t-distributed stochastic neighbor embedding, DensVM clustering, and matched to CellOntology database. Read More

    The CYCLOCALYX study: Ovulatory cycle affects circulating compartments of the endothelial glycocalyx in blood.
    Am J Reprod Immunol 2017 Oct 11. Epub 2017 Oct 11.
    Division of Gynecological Endocrinology and Reproductive Medicine, Department of Gynecology and Obstetrics, Ludwig-Maximilians University, Munich, Germany.
    Problem: The endothelial glycocalyx (EGX) plays an important role in vascular integrity. Recently, increased levels of EGX components were detected in the circulating blood of healthy pregnant women and were related to the increased tendency to edema formation during gestation. However, the EGX has not yet been systematically studied in non-pregnant women during ovulatory cycles. Read More

    Use of D-dimer measurement to guide anticoagulant treatment in recurrent pregnancy loss associated with antiphospholipid syndrome.
    Am J Reprod Immunol 2017 Dec 11;78(6). Epub 2017 Oct 11.
    Department of Reproductive Immunology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, China.
    Problem: To examine whether the level of plasma D-dimer can guide anticoagulant treatment in recurrent pregnancy loss (RPL) associated with antiphospholipid syndrome (APS).

    Methods: A total of 1096 RPL women with APS between 2012 and 2015 in a single-center hospital were randomly divided into two groups (group A, 75 mg of low-dose aspirin [LDA] daily; group B, 75 mg of LDA plus 4100 U of low molecular weight heparin [LMWH] subcutaneously daily); 1015 of the total successfully completed the trial. Plasma D-dimer level and live birth rates were estimated. Read More

    Modulation of Th17 and regulatory T-cell responses during murine pregnancy contributes to increased maternal susceptibility to Salmonella Typhimurium infection.
    Am J Reprod Immunol 2017 Dec 9;78(6). Epub 2017 Oct 9.
    Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada.
    Problem: Salmonella Typhimurium (S. Tm) infection in pregnant mice results in massive placental infection, fetal loss, and exacerbated systemic infection. The Th17 host response can aid control of S. Read More

    Involvement of the JAK-STAT pathway in collagen regulation of decidual NK cells.
    Am J Reprod Immunol 2017 Dec 4;78(6). Epub 2017 Oct 4.
    Hospital and Institute of Obstetrics & Gynecology, Fudan University, Shanghai, China.
    Problem: The mechanisms underlying the regulation of decidual natural killer cells (dNKs) at the maternal-fetal interface are unclear.

    Method Of Study: Primary trophoblasts (TROs), decidual stromal cells (DSCs), and dNKs were cocultured, and responses to LAIR-2 (LAIR-1 inhibitor) and P4H shRNA (collagen inhibitor) were studied.

    Results: Coculture of dNKs with primary TROs/DSCs resulted in downregulation of Th1 cytokine production by dNKs. Read More

    Distinct peripheral vs mucosal T-cell phenotypes in chlamydia-infected women.
    Am J Reprod Immunol 2017 Dec 26;78(6). Epub 2017 Sep 26.
    Division of Infectious Diseases, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.
    Problem: Differences in circulating (peripheral) and mucosal T-cell phenotypes in chlamydia-infected women remain largely unknown.

    Method Of Study: Thirteen paired mononuclear cell specimens from blood and cervicovaginal lavages collected from chlamydia-infected women were stained and analyzed using ten-color cell surface flow cytometry for T-cell distribution, activation status, homing, and T helper (Th)-associated chemokine receptors (CKRs).

    Results: A higher proportion of genital mucosal T-cells were activated (CD38(+) HLA-DR(+) ) and expressed CCR5 and Th1-associated CKR CXCR3(+) CCR5(+) compared to peripheral T-cells, but a lower proportion of mucosal T-cells expressed homing CKR CCR7, Th-2 associated CKR CCR4, and CXCR3(+) CCR4(+) for both T-cell subsets. Read More

    A Single Nucleotide Polymorphism of DNA methyltransferase 3B gene is a risk factor for recurrent spontaneous abortion.
    Am J Reprod Immunol 2017 Dec 20;78(6). Epub 2017 Sep 20.
    Department of Gynaecology and Obstetrics, Clinical Institute of Medical Genetics, UMC Ljubljana, Ljubljana, Slovenia.
    Problem: Aberrant DNA methylation has been suggested as a potential cause of recurrent spontaneous abortion (RSA). Considering the growing evidence on the important roles of DNA methylation in gametogenesis and early pregnancy, we investigated the potential association of DNA methyltransferase gene polymorphisms (DNMT1 rs2228611, DNMT3A rs1550117, DNMT3B rs1569686) with RSA in Slovenian reproductive couples.

    Method Of Study: A total of 146 couples with ≥3 consecutive spontaneous abortions and 149 control women and men with ≥2 normal pregnancies were included. Read More

    Reduced CD200 expression is associated with altered Th1/Th2 cytokine production in placental trophoblasts from preeclampsia.
    Am J Reprod Immunol 2017 Sep 20. Epub 2017 Sep 20.
    Department of Obstetrics and Gynecology, Louisiana State University Health Sciences Center - Shreveport, Shreveport, LA, USA.
    Problem: To determine if altered trophoblast CD200 and CD200R expressions promote inflammatory cytokine production in preeclamptic placentas.

    Methods Of Study: Placental tissue CD200 and CD200R expressions were determined by immunostaining. Tissue sections from first-, second-, and third-trimester, normal term, and preeclamptic placentas were used. Read More

    Expansion of CD4 phenotype among CD160 receptor-expressing lymphocytes in murine pregnancy.
    Am J Reprod Immunol 2017 Dec 16;78(6). Epub 2017 Sep 16.
    Department of Medical Microbiology and Immunology, Medical School, University of Pecs, Pecs, Hungary.
    Problem: CD160, a cell surface co-receptor, is capable of up- or downregulating cell proliferation, cytotoxicity or cytokine production on lymphocytes. Our aim was to investigate CD160(+) lymphocytes in the periphery and at the maternal-foetal interface during murine pregnancy.

    Method Of Study: CD4(+) , CD8(+) and gamma/delta T-cell phenotype, TIM3 co-expression and cytotoxic activity of CD160(+) lymphocytes of pregnant BALB/c mice were analysed by flow cytometry. Read More

    Successful treatment with intrauterine delivery of dexamethasone for repeated implantation failure.
    Am J Reprod Immunol 2017 Dec 16;78(6). Epub 2017 Sep 16.
    Division of Reproductive Sciences, Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, CT, USA.
    Problem: Effective therapy for endometrial receptivity of patients with repeated embryo implantation failure (RIF) is far undeveloped. Whether intrauterine perfusion of dexamethasone (DXM), local administration of drugs with less systematic side-effects, benefit for embryo implantation by suppressing uterine NK (uNK) cells to improve endometrial receptivity remains unknown.

    Method Of Study: Women with RIF were analyzed for the correlation between the percentage of uNK cells during implantation window and following clinical pregnancy rate to determine the appropriate range of uNK for embryo implantation. Read More

    Transcription factor CCAAT/enhancer-binding protein-β upregulates microRNA, let-7f-1 in human endocervical cells.
    Am J Reprod Immunol 2017 Dec 16;78(6). Epub 2017 Sep 16.
    Division of Molecular Immunology and Microbiology (MIM), National Institute for Research in Reproductive Health (NIRRH), Mumbai, India.
    Problem: In endocervical epithelial cells (End1/E6E7), miRNA let-7f plays an important role in the control of innate immunity. The underlying molecular mechanism for let-7f regulation in these cells remains largely unclear.

    Methods Of Study: let-7f was knocked down in End1/E6E7 cells using siRNA, and differential gene expression was analyzed by microarray. Read More

    The extracellular signal-regulated kinase 1/2 triggers angiogenesis in human ectopic endometrial implants by inducing angioblast differentiation and proliferation.
    Am J Reprod Immunol 2017 Dec 16;78(6). Epub 2017 Sep 16.
    Department of Obstetrics & Gynecology, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.
    Problem: The role of extracellular signal-regulated kinase (ERK)1/2-mediated angiogenesis during endometriotic nidation is unknown. We posit that ERK1/2-induced angioblast differentiation and proliferation promotes ectopic endometrial angiogenesis.

    Methods Of Study: Human eutopic and ectopic endometria were immunostained for total- (T-) or phosphorylated- (P-) ERK1/2 or double-immunostained for P-ERK1/2-CD34 and PCNA-CD34. Read More

    Comparison of the percentages of CD4(+)  CD25(high)  FOXP3(+) , CD4(+)  CD25(low)  FOXP3(+) , and CD4(+)  FOXP3(+) Tregs, in the umbilical cord blood of babies born to mothers with and without preeclampsia.
    Am J Reprod Immunol 2017 Dec 16;78(6). Epub 2017 Sep 16.
    Faculty of Medicine, Benha University, Benha, Egypt.
    Problem: Little is known about how preeclampsia affects regulatory T-cell count and functions in umbilical cord blood of babies born to preeclamptic mothers. Here, we analyze the percentage of CD4(+)  CD25(high)  FOXP3(+) , CD4(+)  CD25(low)  FOXP3(+) , and CD4(+)  FOXP3(+) Tregs, in the umbilical cord blood of babies born to mothers with and without preeclampsia.

    Method Of Study: The percentage of umbilical cord blood CD4(+)  CD25(high)  FOXP3(+) , CD4(+)  CD25(low)  FOXP3(+) , and CD4(+)  FOXP3(+) Tregs were analyzed by flow cytometry. Read More

    Undifferentiated connective tissue diseases and adverse pregnancy outcomes. An undervalued association?
    Am J Reprod Immunol 2017 Dec 16;78(6). Epub 2017 Sep 16.
    Division of Rheumatology, University of Pavia, IRCCS Fondazione Policlinico San Matteo, Pavia, Italy.
    Undifferentiated connective tissue diseases (UCTDs) are a heterogeneous group of disorders characterized by symptoms and signs suggestive of systemic autoimmune rheumatic disease (ARD), but which do not fulfill all the established criteria for definite diagnosis of a condition. Although a third of UCTDs can progress to a definite ARD within months or years, most UCTDs can remain stable for years with minimal disease activity. The annual incidence of UCTD in the general population ranges from 14 to 140 per 100 000 people. Read More

    Chlamydia trachomatis regulates innate immune barrier integrity and mediates cytokine and antimicrobial responses in human uterine ECC-1 epithelial cells.
    Am J Reprod Immunol 2017 Dec 16;78(6). Epub 2017 Sep 16.
    Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA.
    Problem: Chlamydia trachomatis infection is the most common sexually transmitted bacterial infection worldwide and known to increase the risk for HIV acquisition. Few studies have investigated how infection of epithelial cells compromises barrier integrity and antimicrobial response.

    Method Of Study: ECC-1 cells, a human uterine epithelial cell line, were treated with live and heat-killed C. Read More

    CCL19/CCR7 contributes to the pathogenesis of endometriosis via PI3K/Akt pathway by regulating the proliferation and invasion of ESCs.
    Am J Reprod Immunol 2017 Nov 30;78(5). Epub 2017 Aug 30.
    Shenzhen Key Laboratory of Fertility Regulation, Center of Assisted Reproduction and Embryology, The University of Hong Kong - Shenzhen Hospital, Shenzhen, China.
    Problem: The level of CCL19 increased in the peritoneal fluid of women with endometriosis, but the precise mechanism of CCL19/CCR7 in the pathogenesis of endometriosis remains unknown.

    Methods: ELISA and immunohistochemistry were performed to analyze CCL19/CCR7 expressions in peritoneal fluid and endometrium from women with endometriosis (n = 38) and controls (n = 32). Cell proliferation and transwell invasion assays were applied to detect proliferation and invasion of human endometrial stromal cells (ESCs). Read More

    Cortisol inhibits CSF2 and CSF3 via DNA methylation and inhibits invasion in first-trimester trophoblast cells.
    Am J Reprod Immunol 2017 Nov 28;78(5). Epub 2017 Aug 28.
    Department of Obstetrics, Gynecology and Reproductive Biology, College of Human Medicine, Michigan State University, Grand Rapids, MI, USA.
    Problem: Heightened maternal stress affects trophoblast function and increases risk for adverse pregnancy outcomes.

    Methods Of Study: Studies were performed using the first-trimester trophoblast cell line, Sw.71. Read More

    Vitamin D deficiency in an Italian cohort of infertile women.
    Am J Reprod Immunol 2017 Oct 3;78(4). Epub 2017 Aug 3.
    Polymedical Center for Prevention of Recurrent Spontaneous Abortion, Rome, Italy.
    Problem: The purpose of this study was to explore whether vitamin D might be a marker of female primary infertility in association with the presence of autoimmune diseases (ADs).

    Methods: The study was a cross-sectional descriptive study in consecutive outpatients of the Polymedical Center for Prevention of Recurrent Spontaneous Abortion (RSA), in Rome, Italy. Women were eligible if they received a diagnosis of primary infertility or RSA. Read More

    Predicting NK cell subsets using gene expression levels in peripheral blood and endometrial biopsy specimens.
    Am J Reprod Immunol 2017 Sep 3;78(3). Epub 2017 Aug 3.
    Clinical Immunology Laboratory, Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA.
    Problem: In molecular analysis of tissue biopsy specimens, one crucial aspect is characterization of immune cell populations. This is especially important for evaluation of uterine receptivity by assessing levels of lymphocyte populations including CD56(bright) CD16- uterine NK cells and CD56(dim) CD16+ conventional NK cells. Our objective was to investigate whether measuring total RNA transcripts from a tissue specimen would accurately reflect immune cell levels and be a new technique to assess immune cell subsets. Read More

    Intrauterine delivery of subunit vaccines induces a systemic and mucosal immune response in rabbits.
    Am J Reprod Immunol 2017 Nov 3;78(5). Epub 2017 Aug 3.
    Vaccine and Infectious Disease Organization- International Vaccine Centre (VIDO-InterVac), University of Saskatchewan, Saskatoon, SK, Canada.
    Problem: Mucosal vaccines have long been sought after to improve protection though the production of both a mucosal and systemic immune response, and are thought to be particularly effective at the site of induction. Development of such vaccines has, however, been delayed by the general propensity to develop immune tolerance to antigens encountered at mucosal sites. This study aimed to determine whether an appropriately formulated subunit vaccine delivered to the uterine lumen would effectively trigger induction of immunity over tolerance. Read More

    Diversity of progesterone action on lipopolysaccharide-induced expression changes in cultured human cervical fibroblasts according to inflammation and treatment timing.
    Am J Reprod Immunol 2017 Nov 1;78(5). Epub 2017 Aug 1.
    Department of Obstetrics and Gynecology, Nippon Medical School, Tokyo, Japan.
    Problem: The effectiveness of progesterone (P4) treatment for preventing preterm births is unclear. Its effects on the uterine cervix were tested using cultured human uterine cervical fibroblasts (UCFs).

    Method Of Study: UCFs were incubated with lipopolysaccharide (LPS) in the presence or absence of P4 under various conditions. Read More

    Placental pericytes and cytomegalovirus infectivity: Implications for HCMV placental pathology and congenital disease.
    Am J Reprod Immunol 2017 Sep 25;78(3). Epub 2017 Jul 25.
    Department of Microbiology and Immunology, Center for AIDS Health Disparities Research, Meharry Medical College, School of Medicine, Nashville, TN, USA.
    Problem: Placental pericytes are essential for placental microvascular function, stability, and integrity. Mechanisms of human cytomegalovirus (HCMV) pathogenesis incorporating placental pericytes are unknown.

    Method Of Study: HCMV-infected placental tissue was stained by dual-labeled immunohistochemistry. Read More

    Amniotic fluid neutrophils can phagocytize bacteria: A mechanism for microbial killing in the amniotic cavity.
    Am J Reprod Immunol 2017 Oct 13;78(4). Epub 2017 Jul 13.
    Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, MD, USA and, Detroit, MI, USA.
    Problem: Neutrophils are capable of performing phagocytosis, a primary mechanism for microbial killing. Intra-amniotic infection is characterized by an influx of neutrophils into the amniotic cavity. Herein, we investigated whether amniotic fluid neutrophils could phagocytize bacteria found in the amniotic cavity of women with intra-amniotic infection. Read More

    A novel in vitro model of villitis of unknown etiology demonstrates altered placental hormone and cytokine profile.
    Am J Reprod Immunol 2017 Nov 6;78(5). Epub 2017 Jul 6.
    Maternal & Fetal Health Research Centre, Manchester Academic Health Science Centre, University of Manchester, Manchester, UK.
    Problem: Placental dysfunction is present over 50% of cases of stillbirth and fetal growth restriction (FGR). Villitis of unknown etiology (VUE), an inflammatory condition of the placenta characterized by maternal T cell infiltrates in the villous stroma and dysregulation of inflammatory cytokines, is more frequent in FGR and stillbirth.

    Method Of Study: A novel in vitro model of placental inflammation was developed to test the hypothesis that inflammatory cells seen in VUE and/or cytokines impair placental function. Read More

    Increased expression of resistin in ectopic endometrial tissue of women with endometriosis.
    Am J Reprod Immunol 2017 Nov 6;78(5). Epub 2017 Jul 6.
    Department of Obstetrics and Gynecology, Korea University College of Medicine, Seoul, Korea.
    Problem: Inflammation is a key process in the establishment and progression of endometriosis. Resistin, an adipocytokine, has biological properties linked to immunologic functions, but its role in endometriosis is unclear.

    Method Of Study: Resistin gene expression was examined in eutopic and ectopic endometrial tissues from women with (n=25) or without (n=25) endometriosis. Read More

    Proliferation of endogenous regulatory T cells improve the pathophysiology associated with placental ischaemia of pregnancy.
    Am J Reprod Immunol 2017 Nov 6;78(5). Epub 2017 Jul 6.
    Department of Pharmacology, University of Mississippi Medical Center, Jackson, MS, USA.
    Problem: Preeclampsia (PE) is associated with inflammation and decreased Treg cells and IL-10. The reduced uterine perfusion pressure (RUPP) rat model of PE exhibits these characteristics, and we hypothesized that induction of endogenous Tregs by a specific stimulus (CD28 superagonistic monoclonal antibody) would reduce inflammation, vasoactive factors, and hypertension in RUPP rats.

    Method Of Study: RUPP was performed at gestation day (GD) 14; CD28 superagonist was administered intraperitoneally GD15; GD18 carotid catheters were inserted, and GD19 MAP and pup weight, blood, and tissues were collected. Read More

    The role of regulatory T cells in thymectomy-induced autoimmune ovarian disease.
    Am J Reprod Immunol 2017 Oct 29;78(4). Epub 2017 Jun 29.
    Reproductive Medical Center, Department of Obstetrics and Gynecology, Southwest Hospital, Third Military Medical University, Chongqing, China.
    Problem: To evaluate the role of regulatory T (Treg) cells in autoimmune ovarian disease (AOD).

    Method Of Study: AOD model was set up by thymectomy of BALB/C mice on day 3 (d3tx). The variation of T lymphocyte subsets, especially the Treg cells were analyzed in the peripheral blood, spleen, para-aortic, and inguinal lymph nodes in d3tx mice. Read More

    The role of γδ-T cells during human pregnancy.
    Am J Reprod Immunol 2017 Aug 27;78(2). Epub 2017 Jun 27.
    Department of Paediatric and Adolescent Medicine, Li Ka Shing Faculty of Medicine, the University of Hong Kong, HongKong, China.
    Pregnancy is an evolutionarily important and mysterious process. The placenta, as the nutrient and gas exchange organ, plays an essential role during this process. In addition, the interaction between trophoblast and maternal immune cells at the maternal-fetal interface is also associated with successful pregnancy. Read More

    Prior pregnancy and antenatal rubella sero-negativity-evidence of persistent maternal immunologic alteration?
    Am J Reprod Immunol 2017 Sep 27;78(3). Epub 2017 Jun 27.
    Department of Obstetrics & Gynaecology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
    Problem: It is unclear if the immunologic alterations induced by pregnancy could persist.

    Method Of Study: Antenatal rubella sero-negativity was correlated with gravidity, abortions and parity in 112 083 gravidae managed during 1997-2015, with further analysis stratified for factors known to influence rubella serology.

    Results: The 10. Read More

    Identification of genes dysregulated by elevation of microRNA-210 levels in human trophoblasts cell line, Swan 71.
    Am J Reprod Immunol 2017 Nov 27;78(5). Epub 2017 Jun 27.
    Department of Biomedical Technology, Kangwon National University, Chuncheon, Korea.
    Problem: Preeclampsia is a serious pregnancy disorder characterized by gestational hypertension and proteinuria. miR-210 is significantly overexpressed in the placentas of preeclampsia patients.

    Method Of Study: Swan 71 cells, first-trimester human trophoblastic cell line, were transfected with hsa-miR-210-3p oligonucleotides by electroporation. Read More

    An investigation of the relationship between recurrent spontaneous abortion and memory T follicular helper cells.
    Am J Reprod Immunol 2017 Nov 21;78(5). Epub 2017 Jun 21.
    Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
    Problem: Immune tolerance with respect to a semi-allogeneic fetus plays a key role in the establishment of a pregnancy. Memory T follicular helper (Tfh) cells have a central role in the regulation of the adaptive immune response. Much of our knowledge of memory Tfh cells' function comes from immune-related diseases. Read More

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