3,608 results match your criteria American Journal of Reproductive Immunology [Journal]


The Role of In Utero Endotoxin Exposure in The Development of Inflammatory Bowel Disease in Mice.

Am J Reprod Immunol 2020 Jul 14:e13302. Epub 2020 Jul 14.

Department of Human Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Problem: Although early environmental influences are thought to influence the development of inflammatory bowel disease (IBD), little is known about the role of the in utero environment on subsequent IBD risk. We hypothesized that prenatal exposure to bacterial lipopolysaccharide (LPS) could modify the subsequent development of dextran sulfate sodium (DSS)-induced ulcerative colitis in adulthood by influencing the associated cellular and immune response.

Method Of Study: To test this hypothesis, we exposed developing mice in utero to LPS or saline (PBS) at E17. Read More

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http://dx.doi.org/10.1111/aji.13302DOI Listing

COVID-19 and Treg/Th17 imbalance: Potential relationship to pregnancy outcomes.

Am J Reprod Immunol 2020 Jul 14:e13304. Epub 2020 Jul 14.

Institute of Reproductive Health, Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P.R. China.

Caused by a novel type of virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), coronavirus disease 2019 (COVID-19) constitutes a global public health emergency. Pregnant women are considered to have a higher risk of severe morbidity and even mortality due to their susceptibility to respiratory pathogens and their particular immunological state. Several studies assessing SARS-CoV-2 infection during pregnancy reported adverse pregnancy outcomes in patients with severe conditions, including spontaneous abortion, preterm labor, fetal distress, cesarean section, preterm birth, neonatal asphyxia, neonatal pneumonia, stillbirth, and neonatal death. Read More

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http://dx.doi.org/10.1111/aji.13304DOI Listing

Seminal plasma contains TGF-β and CXCL10 but sperm washing before cryopreservation is beneficial for post-thawing sperm motility in Black Bengal goats (Capra hircus).

Am J Reprod Immunol 2020 Jul 13:e13301. Epub 2020 Jul 13.

Department of Surgery and Theriogenology, Faculty of Veterinary, Animal and Biomedical Sciences (FVABS), Sylhet Agricultural University (SAU), Sylhet, 3100, Bangladesh.

Problem Artificial insemination, which requires cryopreservation of semen, is not completely optimized in goats because bucks discharge a small volume of ejaculate and seminal plasma (SP) contains specific proteins that are detrimental to spermatozoa at cryopreservation. However, it is not known the effects of sperm washing (removal of SP) before cryopreservation on the post-thawing frozen spermatozoa of Black Bengal bucks. Moreover, it is completely unknown whether SP of goats contains TGF-β and CXCL10 that have been proven essential for fertility in other mammals. Read More

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http://dx.doi.org/10.1111/aji.13301DOI Listing

Lack of association between glutathione S-transferase M1 and T1 gene polymorphisms and susceptibility to preeclampsia: an updated systematic review and meta-analysis.

Am J Reprod Immunol 2020 Jul 13:e13303. Epub 2020 Jul 13.

Central Library, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.

Insufficient response to oxidative stress in placenta is proposed as a contributing factor for preeclampsia (PE) development. Glutathione S-transferases (GST) have significant role in detoxification processes. Conflicting results were published by several research groups regarding GST T1 and GST M1 deletion polymorphism as risk factors for PE. Read More

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http://dx.doi.org/10.1111/aji.13303DOI Listing

Novel predictive and therapeutic options for better pregnancy outcome in frozen embryo transfer cycles.

Am J Reprod Immunol 2020 Jul 12:e13300. Epub 2020 Jul 12.

Department of Pediatrics, Alpert Medical School of Brown University, Women & Infants Hospital, Providence, RI, USA.

Since 1978, in the first decades of in vitro fertilization (IVF), the use of ovarian hyperstimulation allowed for the development and transfer of multiple embryos. As IVF technology improved, the number of multiple pregnancies increased, which led to gradual reduction in the number of embryos that were transferred. Embryo freezing (vitrification) was recommended to allow subsequent transfer if the fresh cycle was unsuccessful. Read More

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http://dx.doi.org/10.1111/aji.13300DOI Listing

Enhanced local and systemic inflammatory cytokine mRNA expression in women with endometriosis evokes compensatory adaptive regulatory mRNA response that mediates immune suppression and impairs cytotoxicity.

Am J Reprod Immunol 2020 Jul 4:e13298. Epub 2020 Jul 4.

Department of Clinical Microbiology/Infection and Immunology, Umeå University, Umeå, Sweden.

Problem: Endometriosis is a disease characterized by ectopic implantation of endometrium and impaired immune responses. To explore its pathogenic mechanisms, we studied the local and systemic cytokine mRNA profiles and their role in the immunity of patients with endometriosis and healthy controls.

Method Of Study: mRNA for eleven cytokines defining cytotoxic Th1, humoral Th2, regulatory Tr1/Th3, and inflammatory cytokine profiles was characterized locally in endometriotic tissue and endometrium, and systemically in PBMCs from women with endometriosis and healthy controls, using real-time qRT-PCR. Read More

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http://dx.doi.org/10.1111/aji.13298DOI Listing

Pregnancy with Covid-19: Management considerations for care of severe and critically ill cases.

Am J Reprod Immunol 2020 Jul 4:e13299. Epub 2020 Jul 4.

Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.

Pregnant women are a potentially highly vulnerable population due to anatomical, physiological and immunological changes under the Covid-19 pandemic. Issues related to pregnancy with Covid-19 attracted widespread attention from researchers. A large number of articles were published aiming to elaborate clinical characteristics and outcomes of pregnant women infected with Covid-19, in order to provide evidence for management. Read More

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http://dx.doi.org/10.1111/aji.13299DOI Listing

Placental Extracellular Vesicles and Preeclampsia.

Am J Reprod Immunol 2020 Jul 3:e13297. Epub 2020 Jul 3.

Department of Pediatrics, Women and Infants Hospital of Rhode Island, Providence, Rhode Island, USA.

Preeclampsia is a hypertensive disease of pregnancy characterized by new-onset hypertension, with either proteinuria and/or organ dysfunction. Preeclampsia is a leading cause of maternal morbidity and mortality; however, the underlying cellular and molecular mechanisms are not well understood. There is consensus that the underlying mechanism(s) resulting in preeclampsia is centered around abnormal placentation, inadequate spiral-artery remodeling, and deficiency in trophoblast invasion, resulting in impaired maternal blood flow to the placenta and a release of signals and/or inflammatory mediators into maternal circulation triggering the systemic manifestations of preeclampsia. Read More

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http://dx.doi.org/10.1111/aji.13297DOI Listing

Soluble immune checkpoints CTLA-4, HLA-G, PD-1, and PD-L1 are associated with endometriosis-related infertility.

Am J Reprod Immunol 2020 Jun 27:e13296. Epub 2020 Jun 27.

Department of Obstetrics and Gynecology, Faculty of Medicine, Airlangga University, Surabaya, Indonesia.

Problem: Soluble immune checkpoint molecules constitute the emerging novel mediators in immune regulation. Their role in the pathogenesis of endometriosis has not been fully addressed. In this study, we aimed to investigate the relationship between the clinical manifestation of endometriosis-associated infertility and the level of four soluble immune checkpoints: sCTLA4, sHLA-G, sPD-1, and sPD-L1. Read More

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Changes in subsets of immunocytes in endometrial hyperplasia.

Am J Reprod Immunol 2020 Jun 24:e13295. Epub 2020 Jun 24.

NHC Key Lab of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, China.

Problem: Endometrial hyperplasia (EH) is characterized by an endometrial gland-to-stroma ratio >1 and is one of the most common gynecological diseases in the world. The role of immunocyte subsets in the development of EH remains unknown.

Methods: Patients who underwent dilatation and curettage due to abnormal uterine bleeding were recruited in the present study. Read More

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http://dx.doi.org/10.1111/aji.13295DOI Listing

Decidual memory T-cell subsets and memory T-cell stimulatory cytokines in early- and late-onset preeclampsia.

Am J Reprod Immunol 2020 Jun 23:e13293. Epub 2020 Jun 23.

Department of Obstetrics and Gynecology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.

Problem: Preeclampsia is a major cause of fetal and maternal mortality and morbidity. Disturbed fetal-maternal immune tolerance, and therewith memory T cells, might be involved in its etiology. This study aims to give insight into memory T-cell populations and its associated cytokines in the decidual layers in early-onset preeclampsia (EO-PE) and late-onset preeclampsia (LO-PE). Read More

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http://dx.doi.org/10.1111/aji.13293DOI Listing

Faster waning of the rubella specific immune response in young pregnant women immunized with MMR at 15 months.

Am J Reprod Immunol 2020 Jun 22:e13294. Epub 2020 Jun 22.

Department of Education and Research, Fooyin University Hospital, Pingtung, Taiwan.

Problem: Vaccination is the best protection against rubella and congenital rubella infection. Although a high rate of immunization coverage is achieved in Taiwan, it is unknown if the vaccine induced immunity persists from the age of vaccination to childbearing age.

Methods Of Study: A total of 5,988 prenatal rubella IgG test results of young pregnant women aged 19-23 years old from six hospitals during Jan. Read More

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Intralipid treatment for women with reproductive failures.

Authors:
Carolyn B Coulam

Am J Reprod Immunol 2020 Jun 22:e13290. Epub 2020 Jun 22.

Clinical Immunology Laboratory, Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA.

Does intravenous intralipid treatment for reproductive failure enhance live births? The answer to this question is yes, BUT only in patients that have a diagnosis of recurrent implantation failure (RIF) or recurrent pregnancy loss (RPL) loss AND demonstrate elevated NK (natural killer) cell density in their endometrial biopsy. Live birth rates have been reported between 33% and 42% among women displaying elevated NK activity with a diagnosis of RIF and 75% and 91% among women experiencing RPL after intralipid infusion. When the pregnancy outcomes of women with a history of reproductive failure and elevated NK cells treated with intralipid were evaluated, the overall live birth rate per cycle of treatment was 61%. Read More

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Dysregulated cytokine profile associated with biochemical premature ovarian insufficiency.

Am J Reprod Immunol 2020 Jun 21:e13292. Epub 2020 Jun 21.

Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China.

Problem: Premature ovarian insufficiency (POI) imposes great challenge on female reproduction. Whether immune disturbance in ovarian environment was implicated in POI remains unclear. We aimed to characterize the cytokine profile in follicular fluid (FF) and paired serum in patients with biochemical POI (bPOI). Read More

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http://dx.doi.org/10.1111/aji.13292DOI Listing

Antinuclear antibodies in follicular fluid may reduce efficacy of in vitro fertilization and embryo transfer by invading endometrium and granular cells.

Am J Reprod Immunol 2020 Jun 21:e13289. Epub 2020 Jun 21.

Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.

Problem: The mechanism(s) by which antinuclear antibodies (ANA) induce implantation failure are not clear, and little information regarding the function of autoantibodies in reproductive tissues is available.

Methods Of Study: A total of 380 patients who underwent in vitro fertilization and embryo transfer (IVF-ET) were divided into control, serum positive, and follicular fluid (FF) positive groups based on the results of indirect immunofluorescence assay for ANA in the serum and FF. Immunofluorescence assay was performed to evaluate the existence of ANA in granular cells and endometrial tissues. Read More

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http://dx.doi.org/10.1111/aji.13289DOI Listing

Natural killer cells and reproductive success.

Am J Reprod Immunol 2020 Jun 20:e13291. Epub 2020 Jun 20.

University of New South Wales, Sydney, Australia.

Natural killer (NK) cell assessment has been attempted since the 1990s and, apart from antibody testing, is probably the commonest immune test available to clinicians. It is clear that simple enumeration of uterine NK cells by immunohistochemistry is inadequate, although better methodology such as flow cytometry may prove to be more beneficial in the future. Blood testing is an appealing noninvasive test that may be a marker for immune dysfunction, rather than a guide to uterine numbers per se. Read More

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http://dx.doi.org/10.1111/aji.13291DOI Listing

Sustained maternal antibody and cellular immune responses in pregnant women infected with Zika virus and mother to infant transfer of Zika-specific antibodies.

Am J Reprod Immunol 2020 Jun 17:e13288. Epub 2020 Jun 17.

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.

Problem: Evaluation of Zika virus (ZIKV)-specific humoral and cellular immune response in pregnant women exposed to ZIKV.

Method Of Study: In this observational, prospective cohort study, we recruited pregnant women presenting for prenatal ultrasound for ZIKV exposure at a single academic teaching hospital in Boston, MA from November 2016 to December 2018. We collected blood, urine, and cervicovaginal swabs antepartum, intrapartum, and postpartum; and cord blood and placenta at delivery. Read More

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http://dx.doi.org/10.1111/aji.13288DOI Listing
June 2020
2.438 Impact Factor

Testicular inflammation and infertility: Could chlamydial infections be contributing?

Am J Reprod Immunol 2020 Jun 13:e13286. Epub 2020 Jun 13.

School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Qld, Australia.

Despite the global incidence of both male infertility and sexually transmitted infections rising each year, the relationship between the two is relatively unstudied. Chlamydia is the most common bacterial sexually transmitted pathogen; however, the majority of research remains focussed on women, while the role of infection and resulting immunopathology in male factor infertility is largely unknown. Chlamydia was found in testicular biopsies from asymptomatic men with idiopathic infertility, which highlights this potential role. Read More

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http://dx.doi.org/10.1111/aji.13286DOI Listing
June 2020
2.438 Impact Factor

Zim CHIC: A cohort study of immune changes in the female genital tract associated with initiation and use of contraceptives.

Am J Reprod Immunol 2020 Jun 13:e13287. Epub 2020 Jun 13.

Department of Obstetrics, Gynecology, and Reproductive Sciences and Center for Family Planning Research, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Problem: Contraceptive hormones are systemically active, potent, and likely to invoke biological responses other than known fertility regulation impacts. We hypothesized that initiation of depot medroxyprogesterone acetate (DMPA) would increase genital HIV-target-cells and soluble immune mediators compared with baseline and initiation of other contraceptive methods.

Method Of Study: We collected cervical cytobrushes and cervicovaginal fluid from healthy Zimbabwean women aged 18-34 to assess immune cell populations, cytokines, and innate anti-HIV activity at baseline and after 30, 90, and 180 days use of DMPA (n = 38), norethisterone enanthate (n = 41), medroxyprogesterone acetate/estradiol cypionate (n = 36), levonorgestrel implant (n = 43), etonogestrel implant (n = 47), or copper intrauterine device (Cu-IUD) (n = 45). Read More

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http://dx.doi.org/10.1111/aji.13287DOI Listing
June 2020
2.438 Impact Factor

Identification of unique clusters of T, dendritic, and innate lymphoid cells in the peritoneal fluid of ovarian cancer patients.

Am J Reprod Immunol 2020 Jun 11:e13284. Epub 2020 Jun 11.

Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Problem: We hypothesize that activated peritoneal immune cells can be redirected to target ovarian tumors. Here, we obtain fundamental knowledge of the peritoneal immune environment through deep immunophenotyping of T cells, dendritic cells (DC), and innate lymphoid cells (ILC) of ovarian cancer patients.

Method Of Study: T cells, DC, and ILC from ascites of ovarian cancer patients (n = 15) and peripheral blood of post-menopausal healthy donors (n = 6) were immunophenotyped on a BD Fortessa cytometer using three panels-each composed of 16 antibodies. Read More

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http://dx.doi.org/10.1111/aji.13284DOI Listing

Immunological environment shifts during pregnancy may affect the risk of developing severe complications in COVID-19 patients.

Am J Reprod Immunol 2020 Jun 9:e13285. Epub 2020 Jun 9.

Institute of Immunology and Physiology, Ural Division of the Russian Academy of Sciences, Ekaterinburg, Russia.

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http://dx.doi.org/10.1111/aji.13285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7300503PMC

Fetal membrane extracellular vesicle profiling reveals distinct pathways induced by infection and inflammation in vitro.

Am J Reprod Immunol 2020 Jun 7:e13282. Epub 2020 Jun 7.

Department of Obstetrics & Gynecology, Division of Maternal-Fetal Medicine & Perinatal Research, The University of Texas Medical Branch at Galveston, Galveston, TX, USA.

Problem: Fetal inflammatory signals can be propagated to maternal tissues to initiate labor via exosomes (extracellular vesicles; 30-150 nm). We tested the hypothesis that fetal membrane cells exposed to infectious and inflammatory mediators associated with preterm birth (PTB) produce exosomes with distinct protein cargo contents indicative of underlying pathobiology.

Methods Of Study: Fetal membrane explants (FM) as well as primary amnion epithelial (AEC) and mesenchymal cells (AMC), and chorion cells (CC) from term deliveries were maintained in normal conditions (control) or exposed to LPS 100 ng/mL or TNF-α 50 ng/mL for 48 hours. Read More

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http://dx.doi.org/10.1111/aji.13282DOI Listing

Erythropoietin prevents LPS-induced preterm birth and increases offspring survival.

Am J Reprod Immunol 2020 Jun 7:e13283. Epub 2020 Jun 7.

Department of Rehabilitation, Guangdong Women and Children Hospital, Guangzhou, China.

Problem: Preterm delivery is the leading cause of neonatal mortality and contributes to delayed physical and cognitive development in children. At present, there is no efficient therapy to prevent preterm labor. A large body of evidence suggests that infections might play a significant and potentially preventable cause of premature birth. Read More

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http://dx.doi.org/10.1111/aji.13283DOI Listing

CXCL12 in normal and pathological pregnancies: A review.

Am J Reprod Immunol 2020 Jun 2:e13280. Epub 2020 Jun 2.

Laboratory for Reproductive Immunology, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, China.

The survival of allogeneic fetuses during pregnancy is a rather paradoxical phenomenon with a complex mechanism. Chemokine ligand12 (CXCL12) and its receptors CXC chemokine receptor (CXCR)4 and 7 are extensively found in placenta tissues and cells, including trophoblast cells, vascular endothelial cells, and decidual stromal and decidual immune cells (eg, NK cells and regulatory T cells). Evidence has illustrated that the CXClL12/CXCR4/CXCR7 axis could enhance the cross talk at the maternal-fetal interface through multiple processes, such as invasion and placental angiogenesis, which appears to be critical signaling components in placentation and fetal outcome. Read More

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http://dx.doi.org/10.1111/aji.13280DOI Listing

CD25 FOXP3 and CD4 CD25 cells distribution in decidual departments of women with severe and mild pre-eclampsia: Comparison with healthy pregnancies.

Am J Reprod Immunol 2020 Jun 2:e13281. Epub 2020 Jun 2.

Laboratory of Morphology, Department of Histology and Embryology, School of Medicine, University of Mostar, Mostar, Bosnia and Herzegovina.

Problem: The aim of this study was to quantify and compare the distribution of regulatory CD25 FOXP3 and activated CD4 CD25 T cells in decidua basalis and parietalis of severe and mild pre-eclampsia (PE) to normal healthy pregnancies.

Method Of Study: Decidual tissue (decidua basalis and parietalis) of 13 women with mild PE, 15 women with severe PE, and 19 women with healthy term pregnancies were analyzed by immunohistochemistry and double immunofluorescence.

Results: The total number of CD25 FOXP3 cells/mm in decidua basalis was decreased in the severe and mild PE versus normal pregnancy group. Read More

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http://dx.doi.org/10.1111/aji.13281DOI Listing

Increased placental macrophages and a pro-inflammatory profile in placentas and maternal serum in infants with a decreased growth rate in the third trimester of pregnancy.

Am J Reprod Immunol 2020 May 18:e13267. Epub 2020 May 18.

Division of Developmental Biology & Medicine, Faculty of Biology, Medicine & Health, Tommy's Maternal and Fetal Health Research Centre, 5th Floor St. Mary's Hospital, University of Manchester, Manchester, UK.

Problem: There is growing evidence for the role of placental inflammation in the pathophysiology of pregnancy complications including fetal growth restriction (FGR). This study aimed to characterize the inflammatory profile in the maternal circulation and the placenta of infants who were growth restricted and those that were small for gestational age (SGA).

Method Of Study: Placental villous tissue and maternal serum were obtained from pregnancies where infants were SGA at birth or who had a decreasing growth rate (≥25 centiles) across the third trimester. Read More

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http://dx.doi.org/10.1111/aji.13267DOI Listing

Myeloid-derived suppressor cells in obstetrical and gynecological diseases.

Am J Reprod Immunol 2020 May 17:e13266. Epub 2020 May 17.

Insitute of Obstetrics and Gynecology, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, China.

Myeloid-derived suppressor cells (MDSCs) are a heterogeneous group of myeloid-origin cells which have immunosuppressive activities in several conditions, such as cancer and inflammation. Recent research has also associated MDSCs with numerous obstetrical and gynecological diseases. During pregnancy, MDSCs accumulate to ensure maternal-fetal immune tolerance, whereas they are decreased in patients who suffer from early miscarriage or pre-eclampsia. Read More

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http://dx.doi.org/10.1111/aji.13266DOI Listing

Controlling chronic low-grade inflammation to improve follicle development and survival.

Am J Reprod Immunol 2020 May 12:e13265. Epub 2020 May 12.

Reproductive Health Department, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen, China.

Chronic low-grade inflammation is one cause of follicle development disturbance. Chronic inflammation exists in pathological conditions such as premature ovarian failure, physiological aging of the ovaries, and polycystic ovary syndrome. Inflammation of the whole body can affect oocytes via the follicle microenvironment, oxidative stress, and GM-CSF. Read More

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http://dx.doi.org/10.1111/aji.13265DOI Listing

HLA antibody repertoire in infants suggests selectivity in transplacental crossing.

Am J Reprod Immunol 2020 May 12:e13264. Epub 2020 May 12.

Centre for Vaccines and Immunology (CVI), National Institute for Communicable Diseases (NICD), A Division of the National Health Laboratory Service, Johannesburg, Gauteng, South Africa.

Problem: Late in pregnancy, women produce and transfer high amounts of antibodies to the foetus. During gestation, women produce antibodies against human leukocyte antigens (HLA), including antibodies directed at foetal HLA. There is paucity of data on transplacental crossing, specificity and role of HLA antibodies in pregnancy and new-borns. Read More

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http://dx.doi.org/10.1111/aji.13264DOI Listing

Transforming growth factor-β1 in intrauterine adhesion.

Am J Reprod Immunol 2020 May 7:e13262. Epub 2020 May 7.

Laboratory for Reproductive Immunology, Institute of Obstetrics and Gynecology, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, China.

Intrauterine adhesion (IUA), led by trauma to the basal layer, can prevent the endometrium from growing, resulting in complications in females, such as infertility and amenorrhea. Transforming growth factor-β1 (TGF-β1) plays a crucial role in inducing and promoting the differentiation and proliferation of mesenchymal cells, in the secretion of extracellular matrix-associated components, and is a major cytokine in initiating and terminating tissue repair downstream of the TGF-β/Smad signaling pathway. Some evidence supports that TGF-β1 is closely associated with the occurrence and development of IUA, and is regarded as an early risk factor of disease recurrence. Read More

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http://dx.doi.org/10.1111/aji.13262DOI Listing

Maternal host responses to poly(I:C) during pregnancy leads to both dysfunctional immune profiles and altered behaviour in the offspring.

Am J Reprod Immunol 2020 May 4:e13260. Epub 2020 May 4.

Experimental Therapeutics Laboratory, School of Pharmacy and Medical Science, UniSA Cancer Research Institute, University of South Australia, Adelaide, SA, Australia.

Problem: Autism spectrum disorder (ASD)-like phenotypes in murine models are linked to elevated pro-inflammatory cytokine profiles caused by maternal immune activation (MIA), but whether MIA alters the immune response in the offspring remains unclear.

Method Of Study: Polyinosinic:polycytidylic acid (poly:[IC]) was used to induce MIA in immunocompetent and control TLR3-deficient pregnant mice, and cytokine levels were measured in maternal and foetal organs. Furthermore, cytokines and behaviour responses were tested after challenge with lipopolysaccharide in 7-day-old and adult mice. Read More

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http://dx.doi.org/10.1111/aji.13260DOI Listing

Decidual stromal cells maintain decidual macrophage homeostasis by secreting IL-24 in early pregnancy.

Am J Reprod Immunol 2020 Apr 30:e13261. Epub 2020 Apr 30.

Insitute of Obstetrics and Gynecology, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, China.

Problem: The state of self-renewal and self-maintain of decidual macrophages would be important for immune homeostasis at the maternal-fetal interface. The roles of interleukin (IL)-24 derived from decidual stromal cells (DSCs) on decidual macrophages have not been explored.

Method Of Study: IL-24 expression in DSCs was interfered by lentivirus, and the transcription levels of IL-24 in DSCs were verified by real time (RT)-PCR. Read More

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http://dx.doi.org/10.1111/aji.13261DOI Listing

Is there a role for HLA-G in the induction of regulatory T cells during the maintenance of a healthy pregnancy?

Am J Reprod Immunol 2020 Apr 30:e13259. Epub 2020 Apr 30.

Department of Microbiology, Immunology and Infectious Diseases, College of Medicine and Medical Sciences, Arabian Gulf University, Manama, Bahrain.

Problem: Pregnancy remains an immune challenge for the uterus that has to adapt to a semi-allogeneic fetus using various regulatory mechanisms. Both HLA-G and regulatory T cells (CD4  CD25  FOXP3  T ) are upregulated in successful pregnancy, but not in abortion. It is unclear if HLA-G plays a role in the upregulation of regulatory cells. Read More

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http://dx.doi.org/10.1111/aji.13259DOI Listing

Anti-phospholipid antibodies and reproductive failures.

Am J Reprod Immunol 2020 Apr 29:e13258. Epub 2020 Apr 29.

Experimental Laboratory of Immunological and Rheumatologic Researches, Istituto Auxologico Italiano, IRCCS, Cusano Milanino, Milan, Italy.

Anti-phospholipid syndrome (APS) recapitulates the link between autoimmunity and pregnancy failure: Acquired anti-phospholipid antibodies (aPL) play a pathogenic role in pregnancy complications. The diagnosis of obstetric APS can easily be pursued when women present with laboratory and clinical features fulfilling the international classification criteria. Standard therapeutic approach to obstetric APS consists in the association of anti-platelet agents and anticoagulants. Read More

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http://dx.doi.org/10.1111/aji.13258DOI Listing

Chronic endometritis and reproductive failure: Role of syndecan-1.

Am J Reprod Immunol 2020 Apr 23:e13255. Epub 2020 Apr 23.

Center for Reproductive Medicine, Drum Tower Clinic Medical College of Nanjing Medical University, Nanjing, China.

Chronic endometritis (CE) is an unusual inflammatory condition characterized by endometrial plasmacyte infiltration. It has a high prevalence in women with reproductive failure. Because of its characteristic localization patterns and molecular functions, syndecan-1 has been identified as a biomarker of plasmacyte, and syndecan-1 immunohistochemistry (IHC) becomes the most dependable diagnostic method for CE. Read More

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http://dx.doi.org/10.1111/aji.13255DOI Listing

Tissue-resident CD8 T memory cells with unique properties are present in human decidua during early pregnancy.

Am J Reprod Immunol 2020 Jul 23;84(1):e13254. Epub 2020 May 23.

Laboratory for Reproductive Immunology, NHC Key Lab of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Hospital of Obstetrics and Gynecology, Fudan University Shanghai Medical College, Shanghai, China.

Problem: Resident memory T (T ) cells reside in the uterus during pregnancy may play an important role in balancing maternal-fetal tolerance with anti-infectious immunity. Although CD8 T and decidual CD8 T cells have been extensively characterized, the properties of decidual CD8 T (dT ) cells remain poorly defined.

Method Of Study: We investigated the heterogeneity, phenotypes, and functions of dT cells, and compared the proportion of dT cells between normal pregnancy and recurrent spontaneous abortion (RSA) using flow cytometry. Read More

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http://dx.doi.org/10.1111/aji.13254DOI Listing
July 2020
2.438 Impact Factor

Glucocorticoid receptor sensitivity in early pregnancy in an African American cohort.

Am J Reprod Immunol 2020 Jul 23;84(1):e13252. Epub 2020 May 23.

Department of Epidemiology, Rollins School of Public Health, Emory University, Atlanta, GA, USA.

Problem: Disruption in homeostatic feedback loops between inflammatory mediators and the hypothalamic-pituitary-adrenal (HPA) axis is a key mechanism linking chronic stress to inflammation and adverse health outcomes, including those occurring during pregnancy. In particular, alterations in glucocorticoid sensitivity may occur as a result of chronic stress, including that due to racial discrimination, and may be implicated in the persistent adverse maternal and infant health outcomes experienced by African Americans. While there are a few large-scale studies in human pregnancy that measure both cytokines and HPA axis hormones, to our knowledge, none directly measure glucocorticoid sensitivity at the cellular level, especially in an African American population. Read More

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http://dx.doi.org/10.1111/aji.13252DOI Listing

Hydatidiform mole in the era of COVID-19 pandemic. Is there an association?

Am J Reprod Immunol 2020 07 5;84(1):e13253. Epub 2020 May 5.

Faculty of Medicine, Department of Medical Microbiology & Immunology, Assiut University, Assiut, Egypt.

Coronavirus disease 2019 (COVID-19) is considered a worldwide pandemic. COVID-19 patients had profound immune dysregulation so they could be susceptible for adverse pregnancy outcomes as hydatidiform mole. In this article, we tried to explain the link between hydatidiform mole and COVID-19. Read More

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http://dx.doi.org/10.1111/aji.13253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235493PMC
July 2020
2.438 Impact Factor

Efficient isolation, culture, purification, and stem cell expression profiles of primary tumor cells derived from uterine cervical squamous cell carcinoma.

Am J Reprod Immunol 2020 Apr 21:e13251. Epub 2020 Apr 21.

Department of Gynecologic Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Problem: Since not too many human uterus cervical squamous cell carcinoma (CSCC) cell lines in existence, efficient isolation, culture, and purification protocols for primary CSCC cells were optimized as a tool for the study of uterus CSCC.

Method Of Study: The protocols for partial multiple enzymatic digestion and explant cell culture were combined and then the resulting mixed cell component cultures were purified by magnetic-activated cell sorting. Colony-forming assay was utilized for detection of cell carcinogenesis potential, and immunofluorescence was used to detect protein expression of CSCC. Read More

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The role of RORγt at maternal-fetal interface during murine pregnancy.

Am J Reprod Immunol 2020 Apr 20:e13250. Epub 2020 Apr 20.

Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA.

Problem: Innate lymphoid cells (ILCs, including NK cells) and their subsets are the most frequent lymphocytes at the maternal-fetal interface (decidua). Recent recognition of extensive ILC subset diversity at mucosal sites and the possible role they might play at different stages of pregnancy poses questions about their composition and lineage stability. Namely, RORγt-dependent ILC3s have been recognized as a key cellular mediator of tissue organization in the gut and secondary lymphoid organs, prompting examination of their distribution and role in decidua during pregnancy. Read More

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http://dx.doi.org/10.1111/aji.13250DOI Listing
April 2020
2.438 Impact Factor

Cytokine mRNA and protein expression by cell cultures of epithelial ovarian cancer-Methodological considerations on the choice of analytical method for cytokine analyses.

Am J Reprod Immunol 2020 Jul 7;84(1):e13249. Epub 2020 May 7.

Department of Clinical Microbiology, Infection and Immunology, Umeå University, Umeå, Sweden.

Problem: To get a comprehensive picture of cytokine expression in health and disease is difficult, cytokines are transiently and locally expressed, and protein analyses are burdened by biological modifications, technical issues, and sensitivity to handling of samples. Thus, alternative methods, based on molecular techniques for cytokine mRNA analyses, are often used. We compared cytokine mRNA and protein expression to evaluate whether cytokine mRNA profiles can be used instead of protein analyses. Read More

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Dose-dependent structural and immunological changes in the placenta and fetal brain in response to systemic inflammation during pregnancy.

Am J Reprod Immunol 2020 Jul 22;84(1):e13248. Epub 2020 May 22.

Integrated Research Center for Fetal Medicine, Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Problem: Systemic maternal inflammation is associated with adverse neonatal sequelae. We tested the hypothesis that IL-1β is a key inflammatory regulator of adverse pregnancy outcomes.

Method Of Study: Pregnant mice were treated with intraperitoneal injections of IL-1β (0, 0. Read More

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Amoxicillin-induced gut dysbiosis influences estrous cycle in mice and cytokine expression in the ovary and the caecum.

Am J Reprod Immunol 2020 Jul 10;84(1):e13247. Epub 2020 May 10.

Laboratório de Imunologia das Doenças Infecciosas e Crônicas, Universidade José do Rosário Vellano, Alfenas, Brazil.

Problem: Gut dysbiosis is caused by several factors, including the use of antibiotics. Since intestinal dysbiosis is associated with a wide range of immunopathological and reproductive conditions, the main goal of this study was to evaluate amoxicillin-induced gut dysbiosis and its influence on the oestrous cycle in mice.

Method Of Study: Mice were treated with amoxicillin or PBS, and faecal microbiota was evaluated by 16S rDNA metagenomic sequencing. Read More

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http://dx.doi.org/10.1111/aji.13247DOI Listing

Parallel studies of mucosal immunity in the reproductive and gastrointestinal mucosa of HIV-infected women.

Am J Reprod Immunol 2020 Jul 8;84(1):e13246. Epub 2020 May 8.

Department of Medical Microbiology and Immunology, University of California, Davis, CA, USA.

Problem: The effects of HIV on the gastrointestinal tract (GIT), including CD4 depletion, epithelial disruption, and collagen deposition, are well documented and only partially reversed by combination antiretroviral therapy (cART). However, the effects of HIV on the female reproductive tract (FRT) are poorly understood, and most studies have focused on ectocervix and vagina without assessing the upper tract. Here, we investigated CD4 T-cell frequency, phenotype, and HIV-specific T-cell responses in the endocervix and endometrium of HIV-infected women, comparing these tissues to the GIT. Read More

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http://dx.doi.org/10.1111/aji.13246DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7302888PMC

A gene signature for immune subtyping of desert, excluded, and inflamed ovarian tumors.

Am J Reprod Immunol 2020 Jul 9;84(1):e13244. Epub 2020 May 9.

National Cancer Institute, Vilnius, Lithuania.

Problem: The current tumor immunology paradigm emphasizes the role of the immune tumor microenvironment and distinguishes several histologically and transcriptionally different immune tumor subtypes. However, the experimental validation of such classification is so far limited to selected cancer types. Here, we aimed to explore the existence of inflamed, excluded, and desert immune subtypes in ovarian cancer, as well as investigate their association with the disease outcome. Read More

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http://dx.doi.org/10.1111/aji.13244DOI Listing

Potential impact of maternal vitamin D status on peripheral blood and endometrium cellular immunity in women with recurrent implantation failure.

Am J Reprod Immunol 2020 Jul 12;84(1):e13243. Epub 2020 May 12.

Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China.

Problem: This study aims to evaluate the modulatory effects of vitamin D on peripheral blood and endometrial cellular immunity in women with recurrent implantation failure (RIF).

Method Of Study: One hundred and fifty-four women with RIF were identified at a fertility center from January 2018 and March 2019. Blood and endometrium samples were collected during the mid-luteal phase before IVF treatment or pregnancy. Read More

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Changes in the Vα7.2+ CD161++ MAIT cell compartment in early pregnancy are associated with preterm birth in HIV-positive women.

Am J Reprod Immunol 2020 Jun 12;83(6):e13240. Epub 2020 Apr 12.

Nuffield Department of Women's & Reproductive Health, University of Oxford, The Women's Centre, John Radcliffe Hospital, Oxford, UK.

Problem: Human immunodeficiency virus (HIV) infection is associated with an increased risk of adverse pregnancy outcomes, including preterm birth (PTB), despite viral suppression with antiretroviral therapy. Mucosal-associated invariant T (MAIT) cells are an immune cell subset involved in antimicrobial immunity at mucosal surfaces. MAIT cells have been found at the maternal-foetal interface, and MAIT cells are typically depleted early in HIV infection. Read More

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http://dx.doi.org/10.1111/aji.13240DOI Listing
June 2020
2.438 Impact Factor

Intrauterine perfusion immunotherapies in recurrent implantation failures: Systematic review.

Am J Reprod Immunol 2020 Jun 18;83(6):e13242. Epub 2020 Apr 18.

Department of Obstetrics and Gynecology, Campinas University (UNICAMP), Campinas, Brazil.

Studies have investigated the gestational outcomes of new immunological therapies in the treatment of patients with recurrent implantation failure (RIF) in assisted reproductive technology (ART). The objective of this article is to assess the current state of evidence available in the literature on intrauterine perfusion immunotherapies in women undergoing ART treatments. By considering the guidelines of Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA), the authors performed systematic review by searching the databases of PubMed/MEDLINE and Scopus using the following key words: "recurrent implantation failure," "intrauterine infusion," "Platelet-Rich Plasma (PRP)," "Peripheral Blood Mononuclear Cells (PBMC)," "Granulocyte Colony-Stimulating Factor (G-CSF)," and "Human Chorionic Gonadotropin (hCG). Read More

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http://dx.doi.org/10.1111/aji.13242DOI Listing

Distribution of dendritic cells in the septate uterus: An immunological perspective.

Am J Reprod Immunol 2020 Jun 10;83(6):e13241. Epub 2020 Apr 10.

Department of Obstetrics and Gynecology, Nippon Medical School, Tokyo, Japan.

Problem: Septate uterus is associated with spontaneous abortion. Surgical intervention of the uterine septa (US) is frequently performed following spontaneous abortion; however, immunological mechanisms for spontaneous abortion in patients with septate uterus remain completely unknown.

Method Of Study: A total of 12 women with septate uterus who underwent hysteroscopic metroplasty and 10 women with uterine leiomyoma who underwent total hysterectomy were enrolled as the experimental and control groups, respectively. Read More

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http://dx.doi.org/10.1111/aji.13241DOI Listing