18,171 results match your criteria American Journal Of Clinical Pathology[Journal]


Less Is More.

Am J Clin Pathol 2022 May 24. Epub 2022 May 24.

Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Objectives: Neoadjuvant chemotherapy (NAC) confers a survival advantage for muscle-invasive bladder cancer and is now recommended for chemotherapy-eligible patients. NAC may result in absent gross tumor, and current cystectomy gross examination protocols do not specify approach for these cases.

Methods: We included cystectomies performed from 2010 to 2018, capturing a period pre- and post-NAC recommendations. Read More

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Impeded Delivery of Pathology and Laboratory Medicine Services by Corruption Is Not Unique to Resource-Limited Settings.

Am J Clin Pathol 2022 May 10. Epub 2022 May 10.

Department of Pathology, Yale University School of Medicine, New Haven, CT,  USA.

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Correction.

Authors:

Am J Clin Pathol 2022 May 17. Epub 2022 May 17.

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Prognostic Significance of Three-Tiered World Health Organization Classification of Phyllodes Tumor and Correlation to Singapore General Hospital Nomogram.

Am J Clin Pathol 2022 May 15. Epub 2022 May 15.

Department of Pathology, University of Pittsburgh, UPMC Magee-Womens Hospital, Pittsburgh, PA, USA.

Objectives: Phyllodes tumors (PTs) are categorized by the World Health Organization (WHO) as benign, borderline, and malignant. Singapore General Hospital (SGH) nomogram is a recurrence risk assessment tool for PT, which uses cytologic atypia, mitosis, stromal overgrowth, and the surgical margin status. We studied the prognostic significance of WHO classification and its correlation to the SGH nomogram. Read More

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Adequacy in Endocervical Curettage.

Am J Clin Pathol 2022 May 15. Epub 2022 May 15.

Department of Pathology, Thomas Jefferson University Hospital, Philadelphia, PA, USA.

Objectives: Specimen adequacy is an important quality assurance component of a cervical Papanicolaou test. Although consensus exists on minimal acceptable cellularity for cervical Papanicolaou tests, no such criteria exist for endocervical curettage (ECC) specimens. We sought to identify minimum acceptable cellularity for accurate diagnosis of high-grade dysplasia (HGD) on ECC. Read More

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Diagnostic Impact of Next-Generation Sequencing Panels for Lymphoproliferative Neoplasms on Small-Volume Biopsies.

Am J Clin Pathol 2022 May 12. Epub 2022 May 12.

Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.

Objectives: We investigated the feasibility and utility of next-generation sequencing (NGS)-based targeted somatic mutation panels and IG/TR gene rearrangement assays in the diagnosis of lymphoproliferative disorders (LPDs) in small-volume biopsies.

Materials: We performed a retrospective, single-institution review of all NGS assays requested over a 3-year period by hematopathologists for diagnostic purposes on small-volume biopsies.

Results: We identified 59 small-volume biopsies. Read More

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COVID-19 mRNA Vaccines and False Reactivity in Rapid Plasma Reagin.

Am J Clin Pathol 2022 May 7. Epub 2022 May 7.

Dr DY Patil University, Pune, India.

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Tandem Mass Spectrometry-Based Amyloid Typing Using Manual Microdissection and Open-Source Data Processing.

Am J Clin Pathol 2022 05;157(5):748-757

Department of Laboratory Medicine and Pathology, Seattle, WA, USA.

Objectives: Standard implementations of amyloid typing by liquid chromatography-tandem mass spectrometry use capabilities unavailable to most clinical laboratories. To improve accessibility of this testing, we explored easier approaches to tissue sampling and data processing.

Methods: We validated a typing method using manual sampling in place of laser microdissection, pairing the technique with a semiquantitative measure of sampling adequacy. Read More

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Patterns of Error in Interpretive Pathology.

Am J Clin Pathol 2022 05;157(5):767-773

Department of Pathology, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada.

Objectives: To demonstrate how the educational presentation and targeted review of cases with discrepant interpretive findings between pathologists can raise awareness for specific diagnostic errors through identification of common overarching patterns of error in interpretive pathology.

Methods: We performed a review of 147 surgical pathology and cytopathology cases of discordances from 23 PowerPoint presentations presented between 2010 and 2017. Pathologists and pathology residents, blinded from the official interpretations, were presented each case and surveyed for their own diagnostic assessments. Read More

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The Effectiveness of Dual-Staining Immunohistochemistry in the Detection of Mantle Cell Lymphoma in the Bone Marrow.

Am J Clin Pathol 2022 05;157(5):709-717

Hematology Section, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA.

Objectives: Immunohistochemistry (IHC) is a useful method for mantle cell lymphoma (MCL) detection in the bone marrow (BM). However, recognition of the neoplastic B cells can be challenging, especially when there is low-level disease.

Methods: We examined BM from 105 patients with MCL. Read More

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A Combined Biomarker of Bright CD38 and MYC ≥55% Is Highly Predictive of Double-/Triple-Hit High-Grade B-Cell Lymphoma.

Am J Clin Pathol 2022 May 3. Epub 2022 May 3.

Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA.

Objectives: Diagnosis of high-grade B-cell lymphoma with MYC and BCL2 or BCL6 rearrangements (double-/triple-hit lymphoma [DTHL]) appears to mandate fluorescence in situ hybridization (FISH) testing for all large B-cell lymphoma (LBCL). Given the low incidence of DTHL, we aimed to identify flow cytometry (FC) and immunohistochemistry (IHC) features of DTHL that could be used to develop an optimal screening strategy. This combined FC-IHC approach has not yet been studied. Read More

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The Quantification and Significance of Extramedullary Hematopoiesis Seen on Liver Biopsy Specimens.

Am J Clin Pathol 2022 May 3. Epub 2022 May 3.

Department of Pathology, Molecular and Cell-Based Medicine, New York, NY, USA.

Objectives: Extramedullary hematopoiesis (EMH) may occur in the liver and is often considered pathologic in adults. Many hematologic and nonhematologic disorders are associated with the development of EMH. However, it is unclear whether the presence of EMH is always pathologic. Read More

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Tumor-Infiltrating Lymphocytes Improve Magee Equation-Based Prediction of Pathologic Complete Response in HR-Positive/HER2-Negative Breast Cancer.

Am J Clin Pathol 2022 Apr 29. Epub 2022 Apr 29.

Department of Pathology, West China Hospital, Sichuan University, Chengdu, China.

Objectives: Magee equation 3 (ME3) is predictive of the pathologic complete response (pCR) to neoadjuvant chemotherapy (NAC) in patients with hormone receptor (HR)-positive, human epidermal growth factor receptor 2 (HER2)-negative breast cancer but with insufficient predictive performance. This study was designed to improve predictive ability by combining ME3 with additional clinicopathologic markers.

Methods: We retrospectively enrolled 460 patients with HR-positive/HER2-negative breast cancer from 2 centers. Read More

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Too Sensitive or Just Right?

Am J Clin Pathol 2022 Apr 28. Epub 2022 Apr 28.

Department of Pathology, Virginia Commonwealth University (VCU) School of Medicine, Richmond, VA, USA.

Objectives: To compare the performance of the rabbit monoclonal antihuman CD246 antibody (D5F3 clone) with the established ALK1 clone for immunohistochemical assessment of anaplastic large cell lymphoma (ALCL).

Methods: Archival cases of ALCL (n = 27) were assessed immunohistochemically by use of ALK1 and D5F3 clones under standard Clinical Laboratory Improvement Amendments-compliant conditions. The intensity of cytoplasmic staining (0 = none; 1 = faint; 2 = moderate; 3+ = strong) and proportion of neoplastic cells (0%, <5%, 5%-50%, >50%) were evaluated and compared with clinical ALK break-apart fluorescence in situ hybridization (FISH) assays. Read More

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Underrecognized Patterns of High-Grade Squamous Intraepithelial Lesion on ThinPrep Preparations.

Am J Clin Pathol 2022 Apr 26. Epub 2022 Apr 26.

Weill Cornell Medicine-New York Presbyterian Hospital, New York, NY, USA.

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Distinct Gene Mutations Are Associated With Clinicopathologic Features in Urachal Carcinoma.

Am J Clin Pathol 2022 Apr 25. Epub 2022 Apr 25.

Department Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Objectives: To investigate the gene mutational profile of urachal carcinoma in correlation with its clinicopathologic features.

Methods: We analyzed genetic mutations in 30 cases of urachal carcinoma by next-generation sequencing (NGS) test. Histologic slides and clinical data were reviewed. Read More

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Primary Central Nervous System Anaplastic Large Cell Lymphoma, ALK Positive.

Am J Clin Pathol 2022 Apr 23. Epub 2022 Apr 23.

Department of Pathology, Beth Israel Deaconess Medical Center, Boston, MA, USA.

Objectives: Primary central nervous system anaplastic large cell lymphoma, anaplastic lymphoma kinase positive (primary CNS ALCL, ALK+) is a rare CNS lymphoma whose description is limited to case reports. These tumors have a variable clinical course, and prognosis is primarily determined by age. We present the largest case series to date of primary CNS ALCL, ALK+, with observational data. Read More

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IRF8 as a Novel Marker to Differentiate Between CD30-Positive Large Cell Lymphomas.

Am J Clin Pathol 2022 Apr 23. Epub 2022 Apr 23.

Department of Pathology, Yale New-Haven Hospital, Yale School of Medicine, New Haven, CT, USA.

Objectives: Interferon regulatory factor 8 (IRF8) is a new biomarker shown to be positive in monocytic leukemias as well as in B cells. As a transcription factor, it plays a critical role in pre-B-cell differentiation and induction of tolerance pathways, among other functions. Given the frequent diagnostic dilemma in CD30-positive large cell lymphomas that could resemble both Hodgkin lymphoma and anaplastic large cell lymphoma (ALCL), we sought to determine whether IRF8 can be useful in distinguishing between these neoplasms that require different treatment strategies. Read More

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Clinical Validation of a Circulating Tumor Cell Assay Using Density Centrifugation and Automated Immunofluorescence Microscopy.

Am J Clin Pathol 2022 Apr 23. Epub 2022 Apr 23.

Department of RareCyte, Seattle, WA, USA.

Objectives: The US Food and Drug Administration (FDA)-approved CELLSEARCH assay (Menarini Silicon Biosystems) for circulating tumor cells (CTCs) relies on expression of an epithelial cell adhesion molecule to enrich for CTCs. We sought to validate a CTC assay (RareCyte) for clinical use that instead collects a buffy coat preparation enriched for CTCs.

Methods: Normal peripheral blood specimens spiked with cultured breast and prostate cancer cells and 47 clinical samples were used to validate assay performance. Read More

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ABO Blood Groups Are Not Associated With COVID-19 Disease Incidence and Severity When Correcting for Ethnicity Differences in Blood Type.

Am J Clin Pathol 2022 Apr 11. Epub 2022 Apr 11.

Department of Pathology, University of Colorado-Anschutz Medical Campus, Aurora, CO, USA.

Objectives: To determine if blood type is a risk factor for coronavirus disease 2019 (COVID-19) disease incidence and severity after correcting for ethnicity differences between novel infections and known ABO blood type frequency differences.

Methods: We performed a retrospective analysis on all severe acute respiratory system coronavirus 2 (SARS-CoV-2) infections and disease severity across two major testing sites in Colorado. We evaluated all individuals with a SARS-CoV-2 nucleic acid test (NAT) and a known blood type between March 1, 2020, and June 1, 2020. Read More

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Circulating Cell-Free DNA and DNA Integrity as Molecular Diagnostic Tools in Hepatocellular Carcinoma.

Am J Clin Pathol 2022 Apr 11. Epub 2022 Apr 11.

Department of Clinical Pathology, Mansoura, Egypt.

Objectives: We assessed the ability to use circulating cell-free DNA (cfDNA) and the DNA integrity index (DNAII) to detect the transition from liver cirrhosis (LC) to hepatocellular carcinoma (HCC).

Methods: Circulating cfDNA and DNAII were measured in 50 patients with advanced LC and 50 patients with HCC who were followed for 1 month after transarterial chemoembolization (TACE). Fifty healthy participants served as a control group. Read More

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COVID-19 mRNA Vaccines May Cause False Reactivity in Some Serologic Laboratory Tests, Including Rapid Plasma Reagin Tests.

Am J Clin Pathol 2022 Mar 30. Epub 2022 Mar 30.

Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA, USA.

Objectives: Acute viral infections and some vaccines have been shown to increase false positivity in serologic assays. We assessed if the messenger RNA coronavirus disease 2019 (COVID-19) vaccines could cause false reactivity in common serologic assays in a pilot longitudinal cohort.

Methods: Thirty-eight participants with sera available prevaccination, 2 weeks after each vaccine dose, and monthly thereafter for up to 5 months were tested for common infectious disease serologies and antiphospholipid syndrome (APS) serology markers on the BioPlex 2200, Sure-Vue rapid plasma reagin (RPR), and Macro-Vue RPR. Read More

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SATB2 Expression in Undifferentiated Pleomorphic Sarcomas of Bone.

Am J Clin Pathol 2022 Mar 21. Epub 2022 Mar 21.

Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Objectives: SATB2 is a transcriptional regulator that plays an important role in osteoblastic differentiation. We examined the prevalence and potential significance of SATB2 expression in undifferentiated pleomorphic sarcoma (UPS) of bone.

Methods: We examined 38 cases of bone UPS without osteoid. Read More

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Primary Pancreatic Lymphoma Evaluated by Fine-Needle Aspiration.

Am J Clin Pathol 2022 Mar 21. Epub 2022 Mar 21.

Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Objectives: Primary pancreatic lymphoma (PPL) is rare, mimicking pancreatic ductal adenocarcinoma (PDAC) clinically and radiologically. The aim of this study is to evaluate the clinical, radiologic, and pathological characteristics of PPL diagnosed by fine-needle aspiration (FNA) in our institution.

Methods: Patient clinical, radiologic, and pathological information was collected from the electronic health record system. Read More

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GLUT1 Immunohistochemistry Is a Highly Sensitive and Relatively Specific Marker for Erythroid Lineage in Benign and Malignant Hematopoietic Tissues.

Am J Clin Pathol 2022 Mar 21. Epub 2022 Mar 21.

Department of Pathology, University of Chicago, Chicago, IL, USA.

Objectives: Glucose transporter 1 (GLUT1), a glucose transporter, is an abundant protein in erythrocytes with expression beginning early in erythropoiesis. We sought to evaluate the utility of GLUT1 immunohistochemistry (IHC) as a diagnostic marker for identifying erythroid differentiation in hematopoietic tissues, including neoplastic erythroid proliferations.

Methods: A variety of benign and neoplastic bone marrow biopsy specimens containing variable proportions of erythroid precursors were selected (n = 46, including 36 cases of leukemia). Read More

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Application of Standardized Residual Component Culture Criteria for Suspected Septic Transfusion Reactions Would Increase the Component Culturing Rate at a Single Academic Medical Center.

Am J Clin Pathol 2022 Mar 21. Epub 2022 Mar 21.

Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH, USA.

Objectives: The 2019 SCARED study developed the Biomedical Excellence for Safer Transfusion (BEST) criteria in an effort to standardize the decision to culture residual units in the context of suspected septic transfusion reactions (STRs). The goal of this study was to apply the BEST criteria to determine the effect on the transfusion reaction decision to culture.

Methods: This retrospective, single-center, cross-sectional study assessed adult transfusion reactions identified in calendar years 2013 to 2020. Read More

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Clinical, Laboratory, and Histologic Correlates of Serum Antinuclear Antibody in Hispanic Pediatric Patients With Nonalcoholic Fatty Liver Disease.

Am J Clin Pathol 2022 Mar 21. Epub 2022 Mar 21.

Pediatric Gastroenterology, Hepatology and Nutrition, Ochsner Hospital for Children, New Orleans, LA, USA.

Objectives: The significance of antinuclear antibody (ANA) positivity in pediatric Hispanic patients with nonalcoholic fatty liver disease (NAFLD) is unknown.

Methods: ANA status was correlated with clinical, laboratory, and histologic parameters in Hispanic patients with a histologic diagnosis of NAFLD.

Results: Thirty-eight Hispanic children (27 male and 11 female) underwent liver biopsy at a median age of 12. Read More

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