13,275 results match your criteria Amebic Hepatic Abscesses


Mouse neutrophils release extracellular traps in response to Naegleria fowleri.

Parasite Immunol 2018 Dec 7:e12610. Epub 2018 Dec 7.

Laboratorio de Inmunobiología Molecular y Celular, Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, México City, México.

Naegleria fowleri is a free-living amoeba, which is able to infect humans through the nasal mucosa causing a disease in the central nervous system known as primary amoebic meningoencephalitis (PAM). Polymorphonuclear cells (PMNs) play a critical role in the early phase of N. fowleri infection. Read More

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December 2018

-Containing Vacuoles Capture PtdIns(4)-Rich Vesicles Derived from the Golgi Apparatus.

MBio 2018 Dec 11;9(6). Epub 2018 Dec 11.

Institute of Medical Microbiology, University of Zürich, Zürich, Switzerland

is the causative agent of a pneumonia termed Legionnaires' disease. The facultative intracellular bacterium employs the Icm/Dot type IV secretion system (T4SS) and a plethora of translocated "effector" proteins to interfere with host vesicle trafficking pathways and establish a replicative niche, the -containing vacuole (LCV). Internalization of the pathogen and the events immediately ensuing are accompanied by host cell-mediated phosphoinositide (PI) lipid changes and the Icm/Dot-controlled conversion of the LCV from a PtdIns(3)-positive vacuole into a PtdIns(4)-positive replication-permissive compartment, which tightly associates with the endoplasmic reticulum. Read More

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December 2018

Removal Efficacy of Opportunistic Pathogens and Bacterial Community Dynamics in Two Drinking Water Treatment Trains.

Small 2018 Dec 7:e1804436. Epub 2018 Dec 7.

Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University, Shanghai, 200433, China.

Drinking water treatment processes (DWTPs) impact pathogen colonization and microbial communities in finished water; however, their efficacies against opportunistic pathogens are not fully understood. In this study, the effects of treatment steps on the removal of Legionella spp., Legionella pneumophila, nontuberculous mycobacteria, Mycobacterium avium, and two amoeba hosts (Vermamoeba vermiformis, Acanthamoeba) are evaluated in two parallel trains of DWTPs equipped with different pretreatment units. Read More

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December 2018

Diversity of Free-Living Environmental Bacteria and Their Interactions With a Bactivorous Amoeba.

Front Cell Infect Microbiol 2018 23;8:411. Epub 2018 Nov 23.

Queller/Strassmann Laboratory, Washington University in St. Louis, Department of Biology, St. Louis, MO, United States.

A small subset of bacteria in soil interact directly with eukaryotes. Which ones do so can reveal what is important to a eukaryote and how eukaryote defenses might be breached. Soil amoebae are simple eukaryotic organisms and as such could be particularly good for understanding how eukaryote microbiomes originate and are maintained. Read More

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November 2018

Anti-Parasitic Activity of Auranofin Against Pathogenic Naegleria fowleri.

J Eukaryot Microbiol 2018 Dec 5. Epub 2018 Dec 5.

Department of Veterinary Microbiology and Preventive Medicine, Ames, Iowa, 50011.

We report that the gold containing anti-rheumatoid drug auranofin is amoebicidal against human pathogenic Naegleria fowleri. Treatment of N. fowleri cultures at biologically relevant concentrations of 0. Read More

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December 2018

Force field development phase II: Relaxation of physics-based criteria… or inclusion of more rigorous physics into the representation of molecular energetics.

Authors:
A T Hagler

J Comput Aided Mol Des 2018 Nov 30. Epub 2018 Nov 30.

Department of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA.

In the previous paper, we reviewed the origins of energy based calculations, and the early science of FF development. The initial efforts spanning the period from roughly the early 1970s to the mid to late 1990s saw the development of methodologies and philosophies of the derivation of FFs. The use of Cartesian coordinates, derivation of the H-bond potential, different functional forms including diagonal quadratic expressions, coupled valence FFs, functional form of combination rules, and out of plane angles, were all investigated in this period. Read More

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November 2018

Biomolecular force fields: where have we been, where are we now, where do we need to go and how do we get there?

J Comput Aided Mol Des 2018 Nov 30. Epub 2018 Nov 30.

Department of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA.

In this perspective, we review the theory and methodology of the derivation of force fields (FFs), and their validity, for molecular simulations, from their inception in the second half of the twentieth century to the improved representations at the end of the century. We examine the representations of the physics embodied in various force fields, their accuracy and deficiencies. The early days in the 1950s and 60s saw FFs first introduced to analyze vibrational spectra. Read More

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November 2018

Data on euglyphid testate amoeba densities, corresponding protozoic silicon pools, and selected soil parameters of initial and forested biogeosystems.

Data Brief 2018 Dec 10;21:1697-1703. Epub 2018 Nov 10.

Leibniz Centre for Agricultural Landscape Research (ZALF), Eberswalder Str. 84, 15374 Müncheberg, Germany.

The dataset in the present article provides information on protozoic silicon (Si) pools represented by euglyphid testate amoebae (TA) in soils of initial and forested biogeosystems. Protozoic Si pools were calculated from densities of euglyphid TA shells and corresponding Si contents. The article also includes data on potential annual biosilicification rates of euglyphid TA at the examined sites. Read More

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December 2018

Optimal and near-optimal exponent-pairs for the Bertalanffy-Pütter growth model.

PeerJ 2018 23;6:e5973. Epub 2018 Nov 23.

Department of Integrative Biology and Biodiversity, Institute of Mathematics, Universität für Bodenkultur Wien, Wien, VIE, Austria.

The Bertalanffy-Pütter growth model describes mass at age by means of the differential equation d/d = * m  -  * . The special case using the von Bertalanffy exponent-pair = 2/3 and = 1 is most common (it corresponds to the von Bertalanffy growth function VBGF for length in fishery literature). Fitting VBGF to size-at-age data requires the optimization of three model parameters (the constants , , and an initial value for the differential equation). Read More

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November 2018
1 Read

Granulomatous amebic encephalitis caused by Acanthamoeba in an immuncompetent child.

Turk J Pediatr 2018 ;60(3):340-343

Division of Infectious Diseases, İstanbul University İstanbul Faculty of Medicine, İstanbul, Turkey.

Sütçü M, Aktürk H, Gülümser-Şişko S, Acar M, Erol OB, Somer A, Bilgiç B, Salman N. Granulomatous amebic encephalitis caused by Acanthamoeba in an immuncompetent child. Turk J Pediatr 2018; 60: 340-343. Read More

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January 2018
1 Read

An Experimental Model of Primary Amoebic Meningoence phalitis Due to in Iran.

Iran J Parasitol 2018 Jul-Sep;13(3):369-372

Dept. of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: The main aim of the present research was to develop the experimental meningo encephalitis due to isolated from geothermal water sources in mice model, November 2017 in Iran.

Methods: was isolated from geothermal water sources in northern Iran. The number of amoebae was adjusted to be 1×10/ml amoebae. Read More

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November 2018
1 Read

Adhesion strategies of Dictyostelium discoideum- a force spectroscopy study.

Nanoscale 2018 Dec 27;10(47):22504-22519. Epub 2018 Nov 27.

Max Planck Institute for Dynamics and Self-Organization, Am Faßberg 17, 37077 Göttingen, Germany.

Biological adhesion is essential for all motile cells and generally limits locomotion to suitably functionalized substrates displaying a compatible surface chemistry. However, organisms that face vastly varying environmental challenges require a different strategy. The model organism Dictyostelium discoideum (D. Read More

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December 2018
3 Reads

The structure of Legionella effector protein LpnE provides insights into its interaction with Oculocerebrorenal syndrome of Lowe (OCRL) protein.

FEBS J 2018 Nov 26. Epub 2018 Nov 26.

Department of Biochemistry, University of Saskatchewan, 107 Wiggins Road, Saskatoon, Saskatchewan, S7N 5E5, Canada.

Legionella pneumophila is a freshwater bacterium that replicates in predatory amoeba and alveolar macrophage. The ability of L. pneumophila to thrive in eukaryotic host cells is conferred by the Legionella containing vacuole (LCV). Read More

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November 2018
1 Read
4.001 Impact Factor

Small-scale Variation of Testate Amoeba Assemblages: the Effect of Site Heterogeneity and Empty Shell Inclusion.

Microb Ecol 2018 Nov 23. Epub 2018 Nov 23.

Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37, Brno, Czech Republic.

Studies on testate amoeba species distribution at small scales (i.e., single peatland sites) are rare and mostly focus on bogs or mineral-poor Sphagnum fens, leaving spatial patterns within mineral-rich fens completely unexplored. Read More

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November 2018
2 Reads

Genomic Signatures Among Acanthamoeba polyphaga Entoorganisms Unveil Evidence of Coevolution.

J Mol Evol 2018 Nov 20. Epub 2018 Nov 20.

Laboratório de Genética Evolutiva Paulo Leminsk, Departamento de Biologia Molecular, Centro de Ciencias Exatas e da Natureza, Universidade Federal da Paraíba, João Pessoa, Brazil.

The definition of a genomic signature (GS) is "the total net response to selective pressure". Recent isolation and sequencing of naturally occurring organisms, hereby named entoorganisms, within Acanthamoeba polyphaga, raised the hypothesis of a common genomic signature despite their diverse and unrelated evolutionary origin. Widely accepted and implemented tests for GS detection are oligonucleotide relative frequencies (OnRF) and relative codon usage (RCU) surveys. Read More

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November 2018
1 Read

species population found in pond water of parks in Mashhad city, Can the physicochemical factors affect it?

MethodsX 2018 24;5:1427-1430. Epub 2018 Oct 24.

Department of Environmental Health, Faculty of Health, Mashhad University of Medical Sciences, Mashhad, Iran.

species are the ubiquitous free-living amoebas that are found worldwide in soil and water. Among spp., can cause primary amebic meningoencephalitis (PAM). Read More

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October 2018
2 Reads

Predation creates unique void layer in membrane-aerated biofilms.

Water Res 2018 Nov 1;149:232-242. Epub 2018 Nov 1.

Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 156 Fitzpatrick Hall, Notre Dame, IN, 46556, USA. Electronic address:

The membrane-aerated biofilm reactor (MABR) is a novel wastewater treatment technology based on oxygen-supplying membranes. The counter diffusion of oxygen and electron donors in MABRs leads to unique behavior, and we hypothesized it also could impact predation. We used optical coherence tomography (OCT), microsensor analyses, and mathematical modeling to investigate predation in membrane-aerated biofilms (MABs). Read More

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November 2018
3 Reads

A diversity of amoebae colonise the gills of farmed Atlantic salmon (Salmo salar) with amoebic gill disease (AGD).

Eur J Protistol 2018 Oct 29;67:27-45. Epub 2018 Oct 29.

CSIRO Agriculture and Food, Integrated Sustainable Aquaculture Production, Queensland Biosciences Precinct, 306 Carmody Road, Brisbane, Queensland 4067, Australia.

Neoparamoeba perurans is the aetiological agent of amoebic gill disease (AGD) in salmonids, however multiple other amoeba species colonise the gills and their role in AGD is unknown. Taxonomic assessments of these accompanying amoebae on AGD-affected salmon have previously been based on gross morphology alone. The aim of the present study was to document the diversity of amoebae colonising the gills of AGD-affected farmed Atlantic salmon using a combination of morphological and sequence-based taxonomic methods. Read More

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October 2018

Protists: Puppet Masters of the Rhizosphere Microbiome.

Trends Plant Sci 2018 Nov 13. Epub 2018 Nov 13.

Institute of Environmental Biology, Ecology & Biodiversity, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands. Electronic address:

The rhizosphere microbiome is a central determinant of plant performance. Microbiome assembly has traditionally been investigated from a bottom-up perspective, assessing how resources such as root exudates drive microbiome assembly. However, the importance of predation as a driver of microbiome structure has to date largely remained overlooked. Read More

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November 2018
4 Reads

Light variability and mixotrophy: Responses of testate amoeba communities and shell δC values to a peatland shading experiment.

Eur J Protistol 2018 Oct 26;67:15-26. Epub 2018 Oct 26.

Earth and Environmental Science Department, Lehigh University, Bethlehem, PA, USA. Electronic address:

Paleoecological records suggest that growing season length and/or cloudiness may affect peatland carbon accumulation and testate amoeba-based environmental reconstructions, highlighting a need to understand how light intensity affects microbial communities. We shaded plots on two peatlands for two years to examine effects on testate amoeba communities, the relative abundance of mixotrophic and heterotrophic testate amoebae, transfer-function performance, and δC values of two species of mixotrophic testate amoebae. Surprisingly, relative abundance of mixotrophic species increased in shade, although compositional changes did not affect transfer-function performance. Read More

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October 2018

Synthetic bovine lactoferrin peptide Lfampin kills trophozoites by necrosis and resolves amoebic intracecal infection in mice.

Biosci Rep 2018 Nov 14. Epub 2018 Nov 14.

Immunology, Instituto de Investigaciones Biomédicas, Av. Universidad 3000, Ciudad Universitaria, UNAM, Ciudad de México, 04510, Mexico

Amoebiasis caused by the protozoan parasite remains a public health problem in developing countries. Previously, we have shown that lactoferrin (Lf) and several Lf-derived peptides exhibit anti-amoebic activity independently of their iron-binding activity. Here, we evaluated the amoebicidal effect of synthetic Lf-derived peptides Lfcin-B, Lfcin 17-30 and Lfampin, analyzed the mechanism of death induced by the peptides and determined their therapeutic effects on murine intestinal amoebiasis. Read More

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November 2018
8 Reads

Improvements to the AMOEBA Force Field by Introducing Anisotropic Atomic Polarizability of the Water Molecule.

J Chem Theory Comput 2018 Nov 28. Epub 2018 Nov 28.

In this work, we have developed an anisotropic polarizable model for the AMOEBA force field that is derived from electrostatic fitting on a gas phase water molecule as the primary approach to improve the many-body polarization model. We validate our approach using small to large water cluster benchmark data sets and ambient liquid water properties and through comparisons to a variational energy decomposition analysis breakdown of molecular interactions for water and water-ion trimer systems. We find that the accounting of anisotropy polarization for a single water molecule demonstrably improves the description of the many-body polarization energy in all cases. Read More

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November 2018
6 Reads

PAS Domain Protein Pas3 Interacts with the Chromatin Modifier Bre1 in Regulating Cryptococcal Morphogenesis.

MBio 2018 Nov 13;9(6). Epub 2018 Nov 13.

Department of Microbiology, University of Georgia, Athens, Georgia, USA

Switching between different morphotypes is an adaptive cellular response in many microbes. In , the yeast-to-hypha transition confers resistance to microbial predation in the soil and is an integral part of its life cycle. Morphogenesis is also known to be associated with virulence, with the filamentous form being immune-stimulatory and protective in mammalian models of cryptococcosis. Read More

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November 2018
4 Reads

Association between Entamoeba histolytica infection and human leukocyte antigen HLA- DRB1.

Ann Med Surg (Lond) 2018 Dec 18;36:71-74. Epub 2018 Oct 18.

Al-Kindy College of Medicine, University of Baghdad, Iraq.

Background: is the parasitic amoeba which caused amebiasis in human and responsible of 100,000 deaths every year. There is currently no vaccine against this parasite. The innate and adaptive immunity are important in protection against infection. Read More

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December 2018
1 Read

A Mutation in the Gene Alters the Physicochemical Properties of the Bacterial Cell Wall and Reduces Survival inside .

Int J Mol Sci 2018 Nov 8;19(11). Epub 2018 Nov 8.

Department of Comparative Anatomy and Anthropology, Institute of Biology, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, Akademicka 19 St., 20-033 Lublin, Poland.

In our previous report, we had shown that the free-living amoeba influenced the abundance, competiveness, and virulence of NZP2213, the microsymbiont of agriculturally important plants of the genus . The molecular basis of this phenomenon; however, had not been explored. In the present study, we demonstrated that , the -acetyltransferase encoding gene located in the lipopolysaccharide (LPS) synthesis cluster of , was responsible for maintaining the protective capacity of the bacterial cell envelope, necessary for the bacteria to fight environmental stress and survive inside amoeba cells. Read More

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November 2018
4 Reads

Calreticulin Induces the Expression of Cytokines in Peripheral Blood Mononuclear Cells Isolated From Patients With Amebic Liver Abscess.

Front Cell Infect Microbiol 2018 18;8:358. Epub 2018 Oct 18.

Laboratorio de Inmunología, Unidad de Investigación de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Ciudad de Mexico, Mexico.

Calreticulin (CRT) is a highly conserved protein in the endoplasmic reticulum that plays important roles in the regulation of key cellular functions. Little is known about the participation of CRT (CRT) in the processes of pathogenicity or in the modulation of the host immune response. The aim of this study was to evaluate the role of CRT in the proliferation and the cytokine profile in peripheral blood mononuclear cells (PBMCs) from patients with amebic liver abscess (ALA) during the acute phase (AP-ALA) of the disease compared to patients during the resolution phase (R-ALA). Read More

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October 2018
3 Reads

Localization of all four ZnT zinc transporters in and impact of ZntA and ZntB knockout on bacteria killing.

J Cell Sci 2018 Nov 30;131(23). Epub 2018 Nov 30.

Department of Biochemistry, Faculty of Science, University of Geneva, 30 Quai Ernest-Ansermet, Science II, 1211 Geneva-4, Switzerland.

Professional phagocytes have developed an extensive repertoire of autonomous immunity strategies to ensure killing of bacteria. Besides phagosome acidification and the generation of reactive oxygen species, deprivation of nutrients and the lumenal accumulation of toxic metals are essential to kill ingested bacteria or inhibit the growth of intracellular pathogens. Here, we used the soil amoeba , a professional phagocyte that digests bacteria for nutritional purposes, to decipher the role of zinc poisoning during phagocytosis of nonpathogenic bacteria and visualize the temporal and spatial dynamics of compartmentalized, free zinc using fluorescent probes. Read More

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November 2018
1 Read

Potentially pathogenic genera of free-living amoebae coexisting in a thermal spring.

Exp Parasitol 2018 Dec 26;195:54-58. Epub 2018 Oct 26.

Departamento de Ciencias Agronómicas y Veterinarias, Mexico; Programa de Doctorado en Ciencias en Biotecnología, Mexico. Electronic address:

Little is known about the prevalence of Balamuthia mandrillaris within the environment due to its difficult isolation, but once an axenic culture is established, it is relatively easy to maintain. As most of the time researchers are interested mainly in isolating B. mandrillaris from environmental samples, the flora that accompanies it becomes second in importance. Read More

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December 2018
1 Read

Gastrointestinal Infection Increases Odds of Inflammatory Bowel Disease in a Nationwide Case-Control Study.

Clin Gastroenterol Hepatol 2018 Oct 30. Epub 2018 Oct 30.

Division of Digestive and Liver Disease, Department of Medicine, Columbia University Medical Center, New York, New York; Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Solna, Sweden; Department of Pediatrics, Orebro University Hospital, Orebro, Sweden; Division of Epidemiology and Public Health, School of Medicine, University of Nottingham, Nottingham, United Kingdom.

Background & Aims: Gastrointestinal infections have been associated with later development of inflammatory bowel diseases (IBD). However, studies have produced conflicting results. We performed a nationwide case-control study in Sweden to determine whether gastroenteritis is associated with the development of Crohn's disease (CD) or ulcerative colitis (UC). Read More

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October 2018
7 Reads

Legionella feeleii: pneumonia or Pontiac fever? Bacterial virulence traits and host immune response.

Med Microbiol Immunol 2018 Nov 1. Epub 2018 Nov 1.

School of Basic Medical Science, Hebei Medical University, Shijiazhuang, 050017, People's Republic of China.

Gram-negative bacterium Legionella is able to proliferate intracellularly in mammalian host cells and amoeba, which became known in 1976 since they caused a large outbreak of pneumonia. It had been reported that different strains of Legionella pneumophila, Legionella micdadei, Legionella longbeachae, and Legionella feeleii caused human respiratory diseases, which were known as Pontiac fever or Legionnaires' disease. However, the differences of the virulence traits among the strains of the single species and the pathogenesis of the two diseases that were due to the bacterial virulence factors had not been well elucidated. Read More

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November 2018
3 Reads

Functional Assessment of 2,177 U.S. and International Drugs Identifies the Quinoline Nitroxoline as a Potent Amoebicidal Agent against the Pathogen .

MBio 2018 Oct 30;9(5). Epub 2018 Oct 30.

Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA

is a pathogenic free-living amoeba that causes a rare but almost always fatal infection of the central nervous system called granulomatous amoebic encephalitis (GAE). Two distinct forms of -a proliferative trophozoite form and a nonproliferative cyst form, which is highly resistant to harsh physical and chemical conditions-have been isolated from environmental samples worldwide and are both observed in infected tissue. Patients suffering from GAE are typically treated with aggressive and prolonged multidrug regimens that often include the antimicrobial agents miltefosine and pentamidine isethionate. Read More

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October 2018
3 Reads

A high-throughput sequencing assay to comprehensively detect and characterize unicellular eukaryotes and helminths from biological and environmental samples.

Microbiome 2018 Oct 29;6(1):195. Epub 2018 Oct 29.

Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA.

Background: Several of the most devastating human diseases are caused by eukaryotic parasites transmitted by arthropod vectors or through food and water contamination. These pathogens only represent a fraction of all unicellular eukaryotes and helminths that are present in the environment and many uncharacterized organisms might have subtle but pervasive effects on health, including by modifying the microbiome where they reside. Unfortunately, while we have modern molecular tools to characterize bacterial and, to a lesser extent, fungal communities, we lack suitable methods to comprehensively investigate and characterize most unicellular eukaryotes and helminths: the detection of these organisms often relies on microscopy that cannot differentiate related organisms, while molecular assays can only detect the pathogens specifically tested. Read More

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October 2018
1 Read

Clinical and microscopic predictors of Entamoeba histolytica intestinal infection in travelers and migrants diagnosed with Entamoeba histolytica/dispar infection.

PLoS Negl Trop Dis 2018 Oct 29;12(10):e0006892. Epub 2018 Oct 29.

Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.

Background: Amebiasis is a protozoal infection caused by Entamoeba histolytica, while the morphologically indistinguishable E. dispar is considered as non-pathogenic. Polymerase chain reaction (PCR) assays are necessary to differentiate both species. Read More

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October 2018
6 Reads

Molecular characterization and phylogenetic analysis of Acanthamoeba isolates in tap water of Beni-Suef, Egypt.

Acta Parasitol 2018 Dec;63(4):826-834

Department of Medical Parasitology, College of Medicine, Beni-Suef University, Beni-Suef, Egypt.

The genus Acanthamoeba is a free-living amoeba widely distributed in various aquatic environments. It is an etiologic cause of amoebic encephalitis and keratitis particularly for immunocompromised individuals. The purpose of the present study was to investigate Acanthamoeba species prevalence in household and hospital potable water in Beni-Suef governorate, Egypt, and to employ sequencing methods to identify positive Acanthamoeba species isolates and their potential health risks. Read More

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December 2018
5 Reads
0.960 Impact Factor

Correlative light electron microscopy of giant viruses with the SECOM system.

New Microbes New Infect 2018 Nov 20;26:110-113. Epub 2018 Sep 20.

APHM, Aix-Marseille Université, IRD, MEPHI, IHU Méditerranée Infection, Marseille, France.

Bright-field transmission electron microscopy (TEM), TEM-negative staining technique, resin-embedding and ultramicrotomy, scanning TEM, scanning electron microscopy, atomic force microscopy and cryoelectron microscopy are imaging techniques used for describing giant viruses, their cycle and ultrastructure. Here we used the SECOM system, an integrated correlative light and electron microscopy using light and electronic imaging without sample transfer, to study cells infected with giant viruses, as shown by the ultrastructure of which was successfully observed. An improvement of the SECOM system with an eye to its use in fundamental and clinical research could be considered in the field of microbiology. Read More

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November 2018
6 Reads

Ultrasound-guided percutaneous catheter drainage of various types of ruptured amebic liver abscess: a report of 117 cases from a highly endemic zone of India.

Abdom Radiol (NY) 2018 Oct 25. Epub 2018 Oct 25.

Department of Gastroenterology, All India Institute of Medical Sciences, Patna, Bihar, India.

Objective: To determine the efficacy and safety ultrasound-guided percutaneous catheter drainage (US-PCD) in management of various types of ruptured amebic liver abscess including free rupture (FR) with diffuse intraperitoneal fluid collections (DIFC).

Methods: This study analyzed 117 patients with ruptured ALA who underwent US-PCD. The indication for US-PCD was failure to respond to conservative treatment and/or percutaneous needle aspiration. Read More

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October 2018
6 Reads

Phenotypic screens reveal posaconazole as rapidly cidal combination partner for treatment of Primary Amoebic Meningoencephalitis.

J Infect Dis 2018 Oct 24. Epub 2018 Oct 24.

Department of Global Health, College of Public Health, University of South Florida, Tampa, FL.

Naegleria fowleri, is the causative agent of primary amoebic meningoencephalitis (PAM), which is fatal in >97% of cases. In this study, we aimed to identify new, rapidly acting drugs to increase survival rates. We conducted phenotypic screens of libraries of FDA-approved compounds and the MMV Pathogen Box and validated 14 hits (IC50s < 1μM). Read More

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October 2018
3 Reads

Clinically Approved Drugs against CNS Diseases as Potential Therapeutic Agents To Target Brain-Eating Amoebae.

ACS Chem Neurosci 2018 Oct 29. Epub 2018 Oct 29.

Department of Biological Sciences, School of Science and Technology , Sunway University , Selangor 47500 , Malaysia.

Central nervous system (CNS) infections caused by free-living amoebae such as Acanthamoeba species and Naegleria fowleri are rare but fatal. A major challenge in the treatment against the infections caused by these amoebae is the discovery of novel compounds that can effectively cross the blood-brain barrier to penetrate the CNS. It is logical to test clinically approved drugs against CNS diseases for their potential antiamoebic effects since they are known for effective blood-brain barrier penetration and affect eukaryotic cell targets. Read More

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October 2018

Isolation and identification of Acanthamoeba from pond water of parks in a tropical and subtropical region in the Middle East, and its relation with physicochemical parameters.

BMC Microbiol 2018 Oct 19;18(1):139. Epub 2018 Oct 19.

Department of Medical Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.

Background: Free-living amoeba (FLA) are wide-spread protozoa that are found in different environmental sources including water, soil, dust, hospital units and ventilation areas. These amoebas can act as opportunistic or non-opportunistic pathogens. Among FLAs, some genera such as Acanthamoeba are important because of their potential pathogenic ability in humans. Read More

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October 2018
7 Reads
2.730 Impact Factor

Identification of free-living amoebae isolated from tap water in Istanbul, Turkey.

Exp Parasitol 2018 Dec 15;195:34-37. Epub 2018 Oct 15.

Molecular Parasitology, Institute of Specific Prophylaxis and Tropical Medicine, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Kinderspitalgasse 15, 1090, Vienna, Austria.

Free-living amoebae (FLA) are widely spread in the environment and also known to cause rare but often serious infections. The present work focuses on a local survey on FLA. It is essential to know the prevalence and distribution of these microorganisms in order to get infections caused by them under control. Read More

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December 2018
6 Reads

A Calcium/Cation Exchanger Participates in the Programmed Cell Death and Virulence of .

Front Cell Infect Microbiol 2018 1;8:342. Epub 2018 Oct 1.

Departamento de Infectómica y Patogénesis Molecular, CINVESTAV-IPN, Mexico City, Mexico.

is the etiologic agent of human amoebiasis, disease that causes 40,000 to 100,000 deaths annually worldwide. The cytopathic activity as well as the growth and differentiation of this microorganism is dependent on both, extracellular and free cytoplasmic calcium. However, few is known about the proteins that regulate the calcium flux in this parasite. Read More

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October 2018
1 Read

Absolute binding free energies for the SAMPL6 cucurbit[8]uril host-guest challenge via the AMOEBA polarizable force field.

J Comput Aided Mol Des 2018 Oct 15;32(10):1087-1095. Epub 2018 Oct 15.

Department of Chemistry, Washington University in St. Louis, Saint Louis, MO, 63130, USA.

As part of the SAMPL6 host-guest blind challenge, the AMOEBA force field was applied to calculate the absolute binding free energy for a cucurbit[8]uril host complexed with 14 diverse guests, ranging from small, rigid structures to drug molecules. The AMOEBA results from the initial submission prompted an investigation into aspects of the methodology and parameterization employed. Lessons learned from the blind challenge include: a double annihilation scheme (electrostatics and van der Waals) is needed to obtain proper sampling of guest conformations, annihilation of key torsion parameters of the guest are recommended for flexible guests, and a more thorough analysis of torsion parameters is warranted. Read More

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October 2018
1 Read

Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes.

J Vis Exp 2018 Sep 27(139). Epub 2018 Sep 27.

2I, UVSQ, INSERM UMR1173, Université Paris-Saclay;

What differentiates Mycobacterium abscessus from other saprophytic mycobacteria is the ability to resist phagocytosis by human macrophages and the ability to multiply inside such cells. These virulence traits render M. abscessus pathogenic, especially in vulnerable hosts with underlying structural lung disease, such as cystic fibrosis, bronchiectasis or tuberculosis. Read More

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September 2018
6 Reads

MmpL8 controls virulence and production of a previously unknown glycolipid family.

Proc Natl Acad Sci U S A 2018 10 9;115(43):E10147-E10156. Epub 2018 Oct 9.

Université de Versailles Saint Quentin en Yvelines, INSERM UMR1173, 78000 Versailles, France;

is a peculiar rapid-growing (RGM) capable of surviving within eukaryotic cells thanks to an arsenal of virulence genes also found in slow-growing mycobacteria (SGM), such as A screen based on the intracellular survival in amoebae and macrophages (MΦ) of an transposon mutant library revealed the important role of MAB_0855, a yet uncharacterized Mycobacterial membrane protein Large (MmpL). Large-scale comparisons with SGM and RGM genomes uncovered MmpL12 proteins as putative orthologs of MAB_0855 and a locus-scale synteny between the and loci. A KO mutant of the gene, designated herein as , had impaired adhesion to MΦ and displayed a decreased intracellular viability. Read More

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October 2018
3 Reads

Fatal Granulomatous Amebic Encephalitis in a Heart Transplant Patient: Clinical, Radiographic, and Autopsy Findings.

J Neuropathol Exp Neurol 2018 Nov;77(11):1001-1004

Department of Pathology, Duke University Medical Center, Durham, North Carolina.

Granulomatous amebic encephalitis is a rare necrotizing infection of the CNS that occurs most commonly in immunocompromised individuals and is usually fatal. It is difficult to diagnose as the clinical symptoms and radiographic findings are often mistaken for other bacterial, viral, fungal, or protozoan infections. Herein, we present the case of a 69-year-old heart transplant recipient who suffered fulminant neurological decline ∼5 months after transplant. Read More

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November 2018
8 Reads

"Tupanvirus", a new genus in the family Mimiviridae.

Arch Virol 2018 Oct 5. Epub 2018 Oct 5.

Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

The genus "Tupanvirus" is a new proposed taxon to be included in the family Mimiviridae. The two known tupanvirus isolates were isolated from soda lake and oceanic sediments samples collected in Brazil and were named "tupanvirus soda lake" and "tupanvirus deep ocean", respectively. These viruses exhibit similarities to amoeba-infecting mimiviruses, but there are also several differences that place them in a separate group within the family Mimiviridae. Read More

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October 2018
2 Reads

Primary Colonizing Play a Key Role in the Growth of in Biofilms on Surfaces Exposed to Drinking Water Treated by Slow Sand Filtration.

Appl Environ Microbiol 2018 Dec 30;84(24). Epub 2018 Nov 30.

Waternet Amsterdam Water Supply, Amsterdam, The Netherlands

Slow sand filtration with extensive pretreatment reduces the microbial growth potential of drinking water to a minimum level at four surface water supplies in The Netherlands. The potential of these slow sand filtrates (SSFs) to promote microbial growth in warm tap water installations was assessed by measuring biofilm formation and growth of bacteria on glass and chlorinated polyvinylchloride (CPVC) surfaces exposed to SSFs at 37 ± 2°C in a model system for up to six months. The steady-state biofilm concentration ranged from 230 to 3,980 pg ATP cm on glass and 1. Read More

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December 2018

Effects of O/Cl disinfection on corrosion and opportunistic pathogens growth in drinking water distribution systems.

J Environ Sci (China) 2018 Nov 3;73:38-46. Epub 2018 Feb 3.

Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.

The effects of O/Cl disinfection on corrosion and the growth of opportunistic pathogens in drinking water distribution systems were studied using annular reactors (ARs). The corrosion process and most probable number (MPN) analysis indicated that the higher content of iron-oxidizing bacteria and iron-reducing bacteria in biofilms of the AR treated with O/Cl induced higher FeO formation in corrosion scales. These corrosion scales became more stable than the ones that formed in the AR treated with Cl alone. Read More

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November 2018
9 Reads

Non-granulomatous cerebellar infection by Acanthamoeba spp. in an immunocompetent host.

Infection 2018 Dec 4;46(6):885-889. Epub 2018 Oct 4.

Hospital Department of Specialized and Internal Medicine, Infectious Diseases Unit, University Hospital of Siena, Siena, Italy.

Acanthamoeba spp. is a free-living amoeba, frequently involved in keratitis by contact lens in immunocompetent hosts. Anecdotal reports associate Acanthamoeba spp. Read More

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December 2018
20 Reads

Rab GTPases take centre stage in understanding Entamoeba histolytica biology.

Small GTPases 2018 Oct 13:1-14. Epub 2018 Oct 13.

d Department of Biological Science , Indian Institute of Science Education and Research Bhopal , Bhauri , India.

Rab GTPases constitute the largest subgroup in the Ras superfamily of GTPases. It is well established that different Rab GTPases are localized in discrete subcellular localization and regulate the membrane trafficking in nearly all eukaryotic cells. Rab GTPase diversity is often regarded as an expression of vesicular trafficking complexity. Read More

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October 2018
2 Reads