13,773 results match your criteria Amebiasis
Front Immunol 2018 26;9:2741. Epub 2018 Nov 26.
Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD, United States.
Leptin, a pleiotropic protein has long been recognized to play an important role in the regulation of energy homeostasis, metabolism, neuroendocrine function, and other physiological functions through its effects on the central nervous system (CNS) and peripheral tissues. Leptin is secreted by adipose tissue and encoded by the obese () gene. Leptin acts as a central mediator which regulates immunity as well as nutrition. Read More
PLoS Pathog 2018 Nov 30;14(11):e1007466. Epub 2018 Nov 30.
Department of Microbiology, Immunology and Infectious Diseases, Snyder Institute for Chronic Diseases, University of Calgary, Calgary, Alberta, Canada.
Amebiasis is caused by the protozoan parasite Entamoeba histolytica (Eh), a potentially fatal disease occurring mainly in developing countries. How Eh interacts with innate host factors in the gut is poorly understood. Eh resides and feed in/on the outer colonic mucus layer and thus share an ecological niche with indigenous microbiota. Read More
J Med Chem 2018 Nov 23. Epub 2018 Nov 23.
Tuberculosis and parasitic diseases, such as giardiasis, amebiasis, leishmaniasis and trypanosomiasis, all urgently require improved treatment options. Recently, it has been shown that anti-tubercular bicyclic nitroimidazoles such as pretomanid and delamanid have potential as repurposed therapeutics for the treatment of visceral leishmaniasis. Here we show that pretomanid also possesses potent activity against Giardia lamblia and Entamoeba histolytica, thus expanding the therapeutic potential of nitroimidazo-oxazines. Read More
J Biol Chem 2018 Nov 19. Epub 2018 Nov 19.
Christian-Albrechts-University of Kiel, Germany.
Microbial formate-nitrite transporter-type proteins (FNT) exhibit dual transport functionality. At neutral pH, electrogenic anion currents are detectable, whereas upon acidification transport of the neutral, protonated monoacid predominates. Physiologically, FNT-mediated proton co-transport is vital when monocarboxylic acid products of the energy metabolism, such as L-lactate, are released from the cell. Read More
J Surg Case Rep 2018 Nov 13;2018(11):rjy297. Epub 2018 Nov 13.
PGY2 Resident General Surgery, U.C.E, Quito, Ecuador.
Amebiasis is still a major healthcare concern, especially in developing countries like Ecuador. The lack of sanitary control and hygiene measures make parasites infections still a burden for patients and physicians. Despite infections due to this parasites are usually mild, severe infections and fatal outcomes although rare still occur. Read More
Biosci Rep 2018 Nov 14. Epub 2018 Nov 14.
Immunology, Instituto de Investigaciones Biomédicas, Av. Universidad 3000, Ciudad Universitaria, UNAM, Ciudad de México, 04510, Mexico
Amoebiasis caused by the protozoan parasite remains a public health problem in developing countries. Previously, we have shown that lactoferrin (Lf) and several Lf-derived peptides exhibit anti-amoebic activity independently of their iron-binding activity. Here, we evaluated the amoebicidal effect of synthetic Lf-derived peptides Lfcin-B, Lfcin 17-30 and Lfampin, analyzed the mechanism of death induced by the peptides and determined their therapeutic effects on murine intestinal amoebiasis. Read More
Front Cell Infect Microbiol 2018 30;8:380. Epub 2018 Oct 30.
Department of Chemistry, Guru Nanak Dev University, Amritsar, India.
Parasitic infections like amebiasis, trichomoniasis, and giardiasis are major health threats in tropical and subtropical regions of the world. Metronidazole (MTZ) is the current drug of choice for amebiasis, giardiasis, and trichomoniasis but it has several adverse effects and potential resistance is a concern. In order to develop alternative antimicrobials, a library of 1-1,2,3-triazole-tethered metronidazole-isatin conjugates was synthesized using Huisgen's azide-alkyne cycloaddition reaction and evaluated for their amebicidal, anti-trichomonal, and anti-giardial potential. Read More
Front Cell Infect Microbiol 2018 29;8:379. Epub 2018 Oct 29.
Science and Healthcare for Oral Welfare, Toulouse, France.
The protozoan resides in the oral cavity and is frequently observed in the periodontal pockets of humans and pets. This species of is closely related to the human pathogen , the agent of amoebiasis. Although is highly enriched in people with periodontitis (a disease in which inflammation and bone loss correlate with changes in the microbial flora), the potential role of this protozoan in oral infectious diseases is not known. Read More
Ann Med Surg (Lond) 2018 Dec 18;36:71-74. Epub 2018 Oct 18.
Al-Kindy College of Medicine, University of Baghdad, Iraq.
Background: is the parasitic amoeba which caused amebiasis in human and responsible of 100,000 deaths every year. There is currently no vaccine against this parasite. The innate and adaptive immunity are important in protection against infection. Read More
Am J Trop Med Hyg 2018 Nov 5. Epub 2018 Nov 5.
Centre for Tropical Diseases, IRCCS Sacro Cuore Don Calabria Hospital, Verona, Italy.
Microscopic examination of stool samples has been considered to be the "gold standard" for diagnosis of intestinal parasites. Recently, polymerase chain reaction (PCR) has been approved by the World Health Organization as the method of choice for the diagnosis of . Of the 106 stool samples collected from the Esmeraldas and Pichincha provinces of Ecuador, all (100%) were positive for / by light microscopy, whereas using real-time PCR (RT-PCR) DNA amplification, 74 (69. Read More
Parasit Vectors 2018 Oct 29;11(1):564. Epub 2018 Oct 29.
Hospital General "Dr. Manuel Gea Gonzalez", Calzada de Tlalpan 4800, Ciudad de Mexico, 14080, Ciudad de Mexico, Mexico.
Background: Blastocystis spp. are the most prevalent intestinal eukaryotes identified in humans, with at least 17 genetic subtypes (ST) based on genes coding for the small-subunit ribosomal RNA (18S). It has been argued that the 18S gene should not be the marker of choice to discriminate between STs of these strains because this marker exhibits high intra-genomic polymorphism. Read More
PLoS Negl Trop Dis 2018 Oct 29;12(10):e0006892. Epub 2018 Oct 29.
Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.
Background: Amebiasis is a protozoal infection caused by Entamoeba histolytica, while the morphologically indistinguishable E. dispar is considered as non-pathogenic. Polymerase chain reaction (PCR) assays are necessary to differentiate both species. Read More
Semin Diagn Pathol 2018 Nov 16;35(6):370-380. Epub 2018 Oct 16.
Department of Pathology, St. Jude Medical Center, Fullerton, CA 92835, United States. Electronic address:
The alimentary tract serves as host to a large number of diseases. In the non-neoplastic group of disorders, conventional histochemistry continues to play an important diagnostic role. It is particularly important in recognizing specific infectious diseases, such as Helicobacter gastritis, Whipple disease, intestinal tuberculosis and other forms of mycobacteriosis, malakoplakia, intestinal spirochetosis, fungal enteritides, amebiasis, cryptosporidiosis, isosporiasis, and microsporidiosis. Read More
Indian J Ophthalmol 2018 Nov;66(11):1623-1624
Department of Microbiology, Dr. Sapra's Lab, Gurugram, Haryana, India.
Semin Immunopathol 2018 Oct 23. Epub 2018 Oct 23.
Department of Molecular Biology and Immunology, Molecular Infection Immunology, Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Straße 74, 20359, Hamburg, Germany.
Parasitic infections modulate the immune system of the host, resulting in either immune tolerance or the induction of pro-inflammatory defense mechanisms against the pathogen. In both cases, sex hormones are involved in the regulation of the immune response, as they are present in the systemic circulation and can act on a wide variety of cell types, including immune cells. Men and women have a different milieu of sex hormones, and these hormones play a role in determining immune responses to parasitic infections. Read More
Med J Malaysia 2018 Oct;73(5):334-335
University of Malaya, Faculty of Medicine, Department of Parasitology, Kuala Lumpur, Malaysia.
Amebiasis is one of the major causes of diarrhea in the developing countries and it can present with a wide range of gastrointestinal symptoms depending on the phase of infection. We described a case of 50 year-old male patient who presented with abdominal pain, diarrhea and vomiting. After right hemicolectomy for appendicular abscess with tumour over the ileum, histopathological examinations revealed numerous trophozoites of Entamoeba histolytica in a background of inflammations (Figure 1). Read More
Technol Cancer Res Treat 2018 Jan;17:1533033818806905
1 Department of General Surgery, School of Medicine, Ruijin Hospital, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Background: Cetuximab has been regularly added to the treatments for metastatic colorectal cancer worldwide. However, due to its therapeutic insensitivity and underlying mechanisms being largely unknown, the clinical implementation of cetuximab in colorectal cancer remains limited.
Methods: The gene expression profile GSE56386 was retrieved from the Gene Expression Omnibus database. Read More
Exp Parasitol 2018 Dec 15;195:34-37. Epub 2018 Oct 15.
Molecular Parasitology, Institute of Specific Prophylaxis and Tropical Medicine, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Kinderspitalgasse 15, 1090, Vienna, Austria.
Free-living amoebae (FLA) are widely spread in the environment and also known to cause rare but often serious infections. The present work focuses on a local survey on FLA. It is essential to know the prevalence and distribution of these microorganisms in order to get infections caused by them under control. Read More
Front Cell Infect Microbiol 2018 2;8:341. Epub 2018 Oct 2.
Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de Mexico, Mexico City, Mexico.
Telomeric Repeat Binding Factors (TRFs) are architectural nuclear proteins with critical roles in telomere-length regulation, chromosome end protection and, fusion prevention, DNA damage detection, and senescence regulation. , the parasite responsible of human amoebiasis, harbors three homologs of human TRFs, based on sequence similarities to their Myb DNA binding domain. These proteins were dubbed EhTRF-like I, II and III. Read More
Genes (Basel) 2018 Oct 16;9(10). Epub 2018 Oct 16.
School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
is an invasive, pathogenic parasite causing amoebiasis. Given that proteins involved in transmembrane (TM) transport are crucial for the adherence, invasion, and nutrition of the parasite, we conducted a genome-wide bioinformatics analysis of encoding proteins to functionally classify and characterize all the TM proteins in . In the present study, 692 TM proteins have been identified, of which 546 are TM transporters. Read More
Front Cell Infect Microbiol 2018 1;8:342. Epub 2018 Oct 1.
Departamento de Infectómica y Patogénesis Molecular, CINVESTAV-IPN, Mexico City, Mexico.
is the etiologic agent of human amoebiasis, disease that causes 40,000 to 100,000 deaths annually worldwide. The cytopathic activity as well as the growth and differentiation of this microorganism is dependent on both, extracellular and free cytoplasmic calcium. However, few is known about the proteins that regulate the calcium flux in this parasite. Read More
PLoS One 2018 16;13(10):e0204867. Epub 2018 Oct 16.
Department of Pathology, Aga Khan University Hospital, Nairobi, Kenya.
Background: Relevant seroprevalence data for endemic pathogens in a given region provide insight not only into a population's susceptibility to acute infection or risk for reactivation disease but also into the potential need for policy initiatives aimed at reducing these risks. Data from sub-Saharan Africa are sparse and since Aga Khan University Hospital Nairobi is an internationally accredited hospital equipped with a laboratory electronic medical record system, analysis of pertinent local seroprevalence data has been made possible.
Methods: We have analyzed serology data from laboratory electronic records at a 300 bed tertiary private teaching hospital in Kenya for the dates, 2008 to 2017 for Toxoplasma gondii, cytomegalovirus, and rubella, which were used primarily for antenatal screening. Read More
Front Cell Infect Microbiol 2018 28;8:340. Epub 2018 Sep 28.
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, Mexico.
In , the EhADH adhesin together with the EhCP112 cysteine protease, form a 124 kDa complex named EhCPADH. This complex participates in trophozoite adherence, phagocytosis and cytolysis of target cells. EhCPADH and EhCP112 are both involved on epithelium damage, by opening tight junctions (TJ) and reaching other intercellular junctions. Read More
PLoS Pathog 2018 Oct 11;14(10):e1007295. Epub 2018 Oct 11.
Department of Molecular Microbiology, Ruth and Bruce Rappaport Faculty of Medicine, Technion, Haifa Israel.
Amebiasis, a global intestinal parasitic disease, is due to Entamoeba histolytica. This parasite, which feeds on bacteria in the large intestine of its human host, can trigger a strong inflammatory response upon invasion of the colonic mucosa. Whereas information about the mechanisms which are used by the parasite to cope with oxidative and nitrosative stresses during infection is available, knowledge about the contribution of bacteria to these mechanisms is lacking. Read More
Rev Soc Bras Med Trop 2018 Sep-Oct;51(5):716-719
Laboratório de Estudos da Relação Parasito-Hospedeiro, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO, Brasil.
We report an Acanthamoeba keratitis case associated with the use of contact lens in a 28-year-old female from Brasília, Brazil. Samples from corneal scraping and contact lens case were used for culture establishment, PCR amplification, and partial sequencing (fragments of ~400kb) of small subunit rDNA; both culture and PCR were positive. The sequence analyses of the cornea and of isolates from the contact lens case showed similarity with the T4 genotype. Read More
Am J Trop Med Hyg 2018 Oct 8. Epub 2018 Oct 8.
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang, Malaysia.
is a protozoan parasite that causes amebiasis and poses a significant health risk for populations in endemic areas. The molecular mechanisms involved in the pathogenesis and regulation of the parasite are not well characterized. We aimed to identify and quantify the differentially abundant membrane proteins by comparing the membrane proteins of virulent and avirulent variants of HM-1:IMSS, and to investigate the potential associations among the differentially abundant membrane proteins. Read More
Trop Med Int Health 2018 Dec 23;23(12):1374-1383. Epub 2018 Oct 23.
Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Objective: To investigate the seroprevalence of Sarcocystosis in the local communities of Pangkor and Tioman islands, Malaysia, by using antigenic recombinant surface antigens 2 and 3 from Sarcocystis falcatula (rSfSAG2 and rSfSAG3) as the target proteins via Western blot and ELISA assays.
Methods: SfSAG2 and SfSAG3 genes were isolated from S. falcatula and expressed in Escherichia coli expression system. Read More
Proteomics 2018 Dec 25;18(23):e1700397. Epub 2018 Oct 25.
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800, Penang, Malaysia.
Entamoeba histolytica membrane proteins are important players toward the pathogenesis of amoebiasis, but the roles of most of the proteins are not fully understood. Since efficient protein extraction method is crucial for a successful MS analysis, three extractions methods are evaluated for the use in studying the membrane proteome of E. histolytica: Two commercial kits (ProteoExtract from Calbiochem and ProteoPrep from Sigma), and a conventional laboratory method. Read More
Front Cell Infect Microbiol 2018 19;8:315. Epub 2018 Sep 19.
Graduate School of Medicine, The University of Tokyo, Bunkyō, Japan.
Eukaryotic cells are organized into separate membrane-bound compartments that have specialized biochemical signature and function. Maintenance and regulation of distinct identity of each compartment is governed by the uneven distribution and intra-cellular movement of two essential biomolecules, lipids, and proteins. Non-vesicular lipid transport mediated by lipid transfer proteins plays a pivotal role in intra-cellular lipid trafficking and homeostasis whereas vesicular transport plays a central role in protein trafficking. Read More
Trop Med Infect Dis 2018 Jun 26;3(3). Epub 2018 Jun 26.
Department of Infectious Diseases, Blacktown Hospital, Blacktown NSW 2148, Australia.
In Australia, amoebiasis is thought to occur in travellers, immigrants from endemic areas, and among men who have sex with men. Prevalence of amoebiasis in communities with immigrants from -endemic countries is unknown. The present study is a retrospective case series analysis of patients with laboratory-confirmed amoebiasis from Western Sydney Local Health District, Australia, between years 2005 and 2016. Read More
Ann Agric Environ Med 2018 Sep 23;25(3):411-416. Epub 2018 Jan 23.
Department of Genetics, Faculty of Biology, University of Szczecin, Poland.
Blastocystis is a unicellular, anaerobic protist which lives in the intestinal tract of diverse animals, including humans. It was found that the host specificity and the pathogenic potential of different isolates are correlated with sequence variations in the SSU-rRNA gene. Identification of the organism to the species level is still an unresolved challenge. Read More
Dis Aquat Organ 2018 Sep;130(3):235-240
Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia and Servei de Diagnòstic Patològic en Peixos, Universitat Autònoma de Barcelona, 08193 Barcelona, Spain.
Systemic amoebiasis of Senegalese sole Solea senegalensis is caused by Endolimax piscium Constenla, Padrós & Palenzuela, 2014 a cryptic parasitic member of the Archamoebae whose epidemiology is yet unknown. To test whether the parasite can be transmitted horizontally, an experimental trial by cohabitation between non-infected and infected fish was designed. Transmission of the parasite from naturally infected to healthy fish was confirmed in the experiment, with the water as the most likely route of infection. Read More
Scand J Gastroenterol 2018 Sep 26;53(9):1085-1088. Epub 2018 Sep 26.
a Department of Gastroenterology and Hepatology , University Hospitals Leuven, KU Leuven , Leuven , Belgium.
Entamoeba histolytica colitis can mimic Crohn's disease. However, a fulminant infection can be life-threatening, especially after exposure to systemic steroids. We present a case of the patient who was initially diagnosed with ileocolonic Crohn's disease, but developed a hepatic E histolytica abscess while undergoing anti-TNF therapy. Read More
Eur J Clin Microbiol Infect Dis 2018 Sep 25. Epub 2018 Sep 25.
Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800, Penang, Malaysia.
Amoebiasis, an enteric protozoan disease caused by Entamoeba histolytica, is a public health problem in many developing countries, causing up to 100,000 fatal cases annually. Detection of the pathogenic E. histolytica and its differentiation from the non-pathogenic Entamoeba spp. Read More
Exp Parasitol 2018 Nov 22;194:38-44. Epub 2018 Sep 22.
Departamento de Biología y Departamento de Farmacia, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta s/n, Guanajuato, Gto, 36050, Mexico. Electronic address:
Amoebiasis is a worldwide health problem caused by the pathogen Entamoeba histolytica. Several virulence factors have been implicated in host invasion, immune evasion, and tissue damage. There are still new factors that remain to be elucidated and characterized. Read More
Gut Microbes 2018 Sep 25:1-5. Epub 2018 Sep 25.
b Division of Infectious Diseases and International Health , University of Virginia , Charlottesville , VA , USA.
Amebiasis, caused by intestinal infection with Entamoeba histolytica, is one of the leading causes of parasite infection-related mortality and morbidity globally. Although its pathogenesis, including determinant factors of infection outcome, remains unclear, recent clinical data indicate that the gut microbiome plays a role in determining the severity of amebiasis. Recently, we investigated the effects of the gut microbiome on neutrophil mediated protection from E. Read More
Future Microbiol 2018 09 21;13:1329-1341. Epub 2018 Sep 21.
Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Av. Instituto Politécnico Nacional 2508, Colonia San Pedro Zacatenco 07360, CdMx, Mexico.
Entamoeba histolytica is a human parasite that causes amoebiasis, a disease that affects the colon and liver and is prevalent worldwide. This protozoan requires a high concentration of iron to survive and reproduce. Iron modulates the expression of parasite virulence factors, including hemoglobinases, hemoglobin-binding proteins and cysteine proteases, as well as proteins related to the amoebic cytoskeleton. Read More
Parasit Vectors 2018 Sep 20;11(1):517. Epub 2018 Sep 20.
Department of Biology and Medical Parasitology, Pomeranian Medical University in Szczecin, 70-204, Szczecin, Poland.
Background: Acanthamoebiasis is most often found in patients with immune deficiency, with infections facilitated by the intake of immunosuppressive drugs. The host immune response to Acanthamoeba spp. infection is poorly understood. Read More
Parasit Vectors 2018 Sep 20;11(1):522. Epub 2018 Sep 20.
Department of Parasitology, Harbin Medical University, Harbin, 150081, Heilongjiang, China.
Background: Blastocystis is one of the most common intestinal parasites in humans and animals worldwide. At least 17 subtypes have been identified in mammals and birds. In China, although some studies have reported the occurrence of Blastocystis in humans and animals, our understanding of the role of animals in the transmission of human blastocystosis is only superficial due to a paucity of available molecular data. Read More
Biomed Rep 2018 Oct 25;9(4):305-312. Epub 2018 Jul 25.
Department of Clinical Laboratory, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China.
Esophageal adenocarcinoma (EAC) is one of the most common subtypes of esophageal cancer, and is associated with a low 5-year survival rate. The present study aimed to identify key genes and pathways associated with EAC using bioinformatics analysis. The gene expression profiles of GSE92396, which includes 12 EAC samples and 9 normal esophageal samples, were downloaded from the Gene Expression Omnibus database. Read More
J Environ Public Health 2018 29;2018:2094621. Epub 2018 Aug 29.
Department of Urban Environmental Management, Kotebe Metropolitan University, P.O. Box 31248, Addis Ababa, Ethiopia.
Considerable fractions of the world's diseases are communicable, of which over 60% are infectious. Knowledge, attitude, and practice of hygiene are very important to decrease these disease burdens especially in places like higher education institutions. This study is aimed at revealing the status and gaps on knowledge, attitude, and practice of hygiene among Kotebe Metropolitan University students. Read More
MBio 2018 Sep 18;9(5). Epub 2018 Sep 18.
Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA
is the etiologic agent of amebic dysentery, though clinical manifestation of infection is highly variable ranging from subclinical colonization to invasive disease. We hypothesize that host genetics contribute to the variable outcomes of infection; thus, we conducted a genome-wide association study (GWAS) in two independent birth cohorts of Bangladeshi infants monitored for susceptibility to disease in the first year of life. Children with at least one diarrheal episode positive for (cases) were compared to children with no detectable infection in the same time frame (controls). Read More
Biosci Rep 2018 Oct 2;38(5). Epub 2018 Oct 2.
Unidad de Investigación en Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México, 04510, México.
Amoebiasis is a parasitic disease caused by This illness is prevalent in poor countries causing 100,000 deaths worldwide. Knowledge of the natural resistance mechanisms of rats to amoebic liver abscess (ALA) development may help to discover new pathogenic factors and to design novel therapeutic strategies against amoebiasis. In this work, histologic analyses suggested that the complement system may play a central role in rat natural resistance to ALA. Read More
Exp Parasitol 2018 Oct 7;193:58-65. Epub 2018 Sep 7.
Department of Biology and Medical Parasitology, Pomeranian Medical University in Szczecin, Poland. Electronic address:
The role of oxidative stress in the pathogenicity of acanthamoebiasis is an important aspect of the intricate and complex host-parasite relationship. The aim of this experimental study was to determine oxidative stress through the assessment of lipid peroxidation product (LPO) levels and antioxidant defense mechanism in Acanthamoeba spp. lung infections in immunocompetent and immunosuppressed hosts. Read More
Front Cell Infect Microbiol 2018 24;8:295. Epub 2018 Aug 24.
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, Mexico.
, the causal agent of human amoebiasis, has two morphologically different phases: a resistant cyst and a trophozoite responsible for the invasion of the host tissues such as the colonic mucosa and the intestinal epithelium. During migration, trophozoites usually produce protuberances such as pseudopods and rarely filopodia, structures that have been observed in the interaction of trophozoites with human colonic epithelial tissue. To study the different membrane projections produced by the trophozoites, including pseudopods, filopodia, uropods, blebs, and others, we designed an induction system using erythrocyte extract or fibronectin (FN) in micropatterned grill lines (each micro-line containing multiple micro-portions of FN or erythrocyte extract) on which the trophozoites were placed in culture for migration assays. Read More
Front Cell Infect Microbiol 2018 17;8:276. Epub 2018 Aug 17.
Division of Infectious Diseases, Department of Internal Medicine, Stanford University School of Medicine, Stanford, CA, United States.
Neglected tropical diseases, especially those caused by parasites, are significantly underserved by current drug development efforts, mostly due to the high costs and low economic returns. One method for lowering the costs of drug discovery and development for these diseases is to repurpose drugs developed for other indications. Here, we present the results of a screen of five repurposed drug libraries to identify potential new lead compounds to treat amebiasis, a disease that affects tens of millions of people and causes ~100,000 deaths annually. Read More
J Biol Chem 2018 Oct 31;293(42):16242-16260. Epub 2018 Aug 31.
From the School of Environmental Sciences,
The 3'-5' exoribonuclease Rrp6 is a key enzyme in RNA homeostasis involved in processing and degradation of many stable RNA precursors, aberrant transcripts, and noncoding RNAs. We previously have shown that in the protozoan parasite , the 5'-external transcribed spacer fragment of pre-rRNA accumulates under serum starvation-induced growth stress. This fragment is a known target of degradation by Rrp6. Read More
FASEB J 2018 Aug 31:fj201801313R. Epub 2018 Aug 31.
Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany; and.
Recently, Entamoeba histolytica clones derived from isolate HM-1:IMSS that differ in their pathogenicity were identified. Whereas some clones induce amoebic liver abscesses (ALAs) in animal models of amoebiasis, others provoke only minimal liver lesions. Based on transcriptome studies of pathogenic and nonpathogenic clones, differentially expressed genes associated with reduced or increased liver pathology can be identified. Read More
Parasitol Int 2018 Dec 27;67(6):824-828. Epub 2018 Aug 27.
Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Bangkok 10400, Thailand. Electronic address:
Blastocystis is a unicellular protist most commonly detected in humans and a variety of animals. The predominant mode of its transmission is the fecal-oral route, but its zoonotic potential is not completely understood. The objective of this study was to determine the presence and genetic diversity of Blastocystis on pig farms in Nakhon Pathom Province, Central Thailand. Read More
Parasitology 2018 Aug 30:1-7. Epub 2018 Aug 30.
Department of Environmental Health,Rollins School of Public Health,Emory University,1518 Clifton Road NE, Atlanta, GA 30322,USA.
Entamoeba histolytica is an enteric parasite that infects approximately 50 million people worldwide. Although E. histolytica is a zoonotic parasite that has the potential to infect nonhuman primates, such transmission is poorly understood. Read More