953 results match your criteria Amb Express[Journal]


Molecular authentication and differentiation of Dendrobium species by rDNA ITS region sequence analysis.

AMB Express 2019 Apr 19;9(1):53. Epub 2019 Apr 19.

State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, People's Republic of China.

Owing to their significant medicinal and edible values, the natural Dendrobium species have underdone over-collection and habitat destruction, and cultivated species emerged for candidates. However, these Dendrobium plants are similar in shape to be easily confused, leading to extreme difficulties for identification based on their morphological and chemical features. In this study, the rDNA ITS region sequence analysis was developed for rapid and accurate identification of thirteen wild and cultivated Dendrobium species belonging to two sections Formosae and Chrysotoxae. Read More

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http://dx.doi.org/10.1186/s13568-019-0767-8DOI Listing

Retraction Note to: Substrates specificity of tannase from Streptomyces sviceus and Lactobacillus plantarum.

AMB Express 2019 Apr 19;9(1):52. Epub 2019 Apr 19.

School of Bioscience and Biotechnology, Chengdu Medical College, 783 Xindu Avenue, Chengdu, 610500, People's Republic of China.

The Editor-in-Chief has retracted this article (Wang et al. 2018) because the authors do not have ownership of the data they report. An investigation by the Commonwealth Scientific and Industrial Research Organisation (CSIRO) has concluded that the data reported in this article are the sole property of the CSIRO. Read More

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http://dx.doi.org/10.1186/s13568-019-0778-5DOI Listing

Rapid detection of Escherichia coli in beverages using genetically engineered bacteriophage T7.

AMB Express 2019 Apr 19;9(1):55. Epub 2019 Apr 19.

Department of Food Science and Technology, University of California, Davis, CA, USA.

Foodborne illness due to bacterial contamination is a significant issue impacting public health that demands new technology which is practical to implement by food industry. Detection of bacteria in food products and production facilities is a crucial strategy supporting food safety assessments. Bacteriophages were investigated as a tool for bacterial detection due to their ability to infect specific strain of host bacteria in order to improve sensitivity, specificity, and rapidity of bacterial detection. Read More

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https://amb-express.springeropen.com/articles/10.1186/s13568
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http://dx.doi.org/10.1186/s13568-019-0776-7DOI Listing
April 2019
1 Read

Inactivation of Listeria monocytogenes in ready-to-eat smoked turkey meat by combination with packaging atmosphere, oregano essential oil and cold temperature.

AMB Express 2019 Apr 19;9(1):54. Epub 2019 Apr 19.

Department of Animal Production, College of Food and Agricultural Sciences, King Saud University, P.O Box 2460, Riyadh, 11451, Saudi Arabia.

The effects of packaging atmosphere, storage temperature and oregano essential oil (EO) on growth of Listeria monocytogenes on ready-to-eat smoked turkey were studied. Smoked turkey slices were inoculated with a strain of Listeria monocytogenes Scott A (5.95, 5. Read More

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http://dx.doi.org/10.1186/s13568-019-0775-8DOI Listing

Antibacterial potential associated with drug-delivery built TiO nanotubes in biomedical implants.

AMB Express 2019 Apr 16;9(1):51. Epub 2019 Apr 16.

Dentistry University, School of Health Sciences, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Av. Ipiranga, P.O. Box 6681, Porto Alegre, 90619-900, Brazil.

The fast evolution of surface treatments for biomedical implants and the concern with their contact with cells and microorganisms at early phases of bone healing has boosted the development of surface topographies presenting drug delivery potential for, among other features, bacterial growth inhibition without impairing cell adhesion. A diverse set of metal ions and nanoparticles (NPs) present antibacterial properties of their own, which can be applied to improve the implant local response to contamination. Considering the promising combination of nanostructured surfaces with antibacterial materials, this critical review describes a variety of antibacterial effects attributed to specific metals, ions and their combinations. Read More

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http://dx.doi.org/10.1186/s13568-019-0777-6DOI Listing

Enhanced calcium carbonate-biofilm complex formation by alkali-generating Lysinibacillus boronitolerans YS11 and alkaliphilic Bacillus sp. AK13.

AMB Express 2019 Apr 11;9(1):49. Epub 2019 Apr 11.

Laboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological Engineering, Korea University, Seoul, 02841, Republic of Korea.

Microbially induced calcium carbonate (CaCO) precipitation (MICP) is a process where microbes induce condition favorable for CaCO formation through metabolic activities by increasing the pH or carbonate ions when calcium is near. The molecular and ecological basis of CaCO precipitating (CCP) bacteria has been poorly illuminated. Here, we showed that increased pH levels by deamination of amino acids is a driving force toward MICP using alkalitolerant Lysinibacillus boronitolerans YS11 as a model species of non-ureolytic CCP bacteria. Read More

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http://dx.doi.org/10.1186/s13568-019-0773-xDOI Listing

In vitro reconstitution and characterisation of the oxidative D-xylose pathway for production of organic acids and alcohols.

AMB Express 2019 Apr 11;9(1):48. Epub 2019 Apr 11.

VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, 02044 VTT, Espoo, Finland.

The oxidative D-xylose pathway, i.e. Dahms pathway, can be utilised to produce from cheap biomass raw material useful chemical intermediates. Read More

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http://dx.doi.org/10.1186/s13568-019-0768-7DOI Listing

High throughput DNA extraction of legume root nodules for rhizobial metagenomics.

Authors:
Gabriel Johnson

AMB Express 2019 Apr 10;9(1):47. Epub 2019 Apr 10.

Department of Botany, National Museum of Natural History, Smithsonian Institution, Museum Support Center, 4210 Silver Hill Road, Suitland, MD, 20746, USA.

A novel combination of tissue homogenization, cell lysis, and DNA purification techniques was developed for isolating total DNA from whole legume root nodules on an automated robotic system. Silica dehydrated root nodules from soy bean, Glycine max cv. Tara, and mung bean, Vigna radiata cv. Read More

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https://amb-express.springeropen.com/articles/10.1186/s13568
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http://dx.doi.org/10.1186/s13568-019-0771-zDOI Listing
April 2019
1 Read

High temperature utilization of PAM and HPAM by microbial communities enriched from oilfield produced water and activated sludge.

AMB Express 2019 Apr 9;9(1):46. Epub 2019 Apr 9.

Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB, T2N 1N4, Canada.

Non-hydrolyzed polyacrylamide (PAM) and partially hydrolyzed polyacrylamide (HPAM) are commonly used polymers in various industrial applications, including in oil and gas production operations. Understanding the microbial utilization of such polymers can contribute to improved recovery processes and help to develop technologies for polymer remediation. Microbial communities enriched from oilfield produced water (PW) and activated sludge from Alberta, Canada were assessed for their ability to utilize PAM and HPAM as nitrogen and carbon sources at 50 °C. Read More

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http://dx.doi.org/10.1186/s13568-019-0766-9DOI Listing

A novel method for the capture-based purification of whole viral native RNA genomes.

AMB Express 2019 Apr 8;9(1):45. Epub 2019 Apr 8.

Genome Institute of Singapore (GIS), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.

Current technologies for targeted characterization and manipulation of viral RNA primarily involve amplification or ultracentrifugation with isopycnic gradients of viral particles to decrease host RNA background. The former strategy is non-compatible for characterizing properties innate to RNA strands such as secondary structure, RNA-RNA interactions, and also for nanopore direct RNA sequencing involving the sequencing of native RNA strands. The latter strategy, ultracentrifugation, causes loss in genomic information due to its inability to retrieve unassembled viral RNA. Read More

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https://amb-express.springeropen.com/articles/10.1186/s13568
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http://dx.doi.org/10.1186/s13568-019-0772-yDOI Listing
April 2019
1 Read

Gut segments outweigh the diet in shaping the intestinal microbiota composition in grass carp Ctenopharyngodon idellus.

AMB Express 2019 Apr 6;9(1):44. Epub 2019 Apr 6.

Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture, and State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.

Although dynamics of the complex microbial ecosystem populating the gastrointestinal tract of animals has profound and multifaceted impacts on host's metabolism and health, it remains unclear whether it is the intrinsic or extrinsic factors that play a more dominant role in mediating the composition of intestinal microbiota. To address this, we studied the impacts of two strikingly different diets on a herbivorous fish, grass carp (Ctenopharyngodon idellus): a high-protein, low-fiber formula feed, and low-protein, high-fiber Sudan grass. After a 16-week feeding trial, microbial profiles of midgut and hindgut segments in both groups were compared. Read More

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https://amb-express.springeropen.com/articles/10.1186/s13568
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http://dx.doi.org/10.1186/s13568-019-0770-0DOI Listing
April 2019
5 Reads

Effect of amphotericin B and voriconazole on the outgrowth of conidia of Aspergillus fumigatus followed by time-lapse microscopy.

AMB Express 2019 Apr 3;9(1):43. Epub 2019 Apr 3.

Department of Molecular Biotechnology and Microbiology, Faculty of Science and Technology, University of Debrecen, 1 Egyetem Square, Debrecen, 4010, Hungary.

Studies of morphological measurements from the outgrowth of cells to a network of hyphae have been extended from Candida albicans (Nagy et al. in Appl Microbiol Biotechnol 98(11):5185-5194. https://doi. Read More

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http://dx.doi.org/10.1186/s13568-019-0769-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447639PMC
April 2019
1 Read

Responses of phyllosphere microbiota and plant health to application of two different biocontrol agents.

AMB Express 2019 Mar 28;9(1):42. Epub 2019 Mar 28.

School of Minerals Processing and Bioengineering, Central South University, Changsha, China.

The phyllosphere supports a tremendous diversity of microbes, which have the potential to influence plant biogeography and ecosystem function. Although biocontrol agents (BCAs) have been used extensively for controlling plant diseases, the ecological effects of BCAs on phyllosphere bacteria and the relationships between phyllosphere community and plant health are poorly understood. In this study, we explored the control efficiency of two BCA communities on bacterial wildfire disease by repeatedly spraying BCAs on tobacco leaves. Read More

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http://dx.doi.org/10.1186/s13568-019-0765-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439047PMC

Chemical profiling, biostatic and biocidal dynamics of Origanum vulgare L. essential oil.

AMB Express 2019 Mar 26;9(1):41. Epub 2019 Mar 26.

Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in Egypt, Cairo, 11835, Egypt.

Origanum vulgare L. (Lamiaceae) is a widespread flavoring culinary and medicinal herb. The present study aimed at investigating the antimicrobial activity of Origanum vulgare (OV) essential oil (EO) through illustrating its biostatic, biocidal and the dynamics of the biocidal activity against 11 different microorganisms. Read More

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http://dx.doi.org/10.1186/s13568-019-0764-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6435767PMC

Altered diversity and composition of the gut microbiome in patients with cervical cancer.

AMB Express 2019 Mar 23;9(1):40. Epub 2019 Mar 23.

Department of Radiation Oncology, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute & Hospital, National Clinical Research Center for Cancer, West Huanhu Road, West River District, Tianjin, 300060, China.

Gut microbiota have been implicated in the development of many human diseases, including both digestive diseases and non-digestive diseases. In this study, we investigated whether the gut bacteria differed in cervical cancer (CCa) patients compared with healthy controls by 16S rRNA sequencing analysis. Subjects including eight CCa and five healthy controls were included. Read More

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http://dx.doi.org/10.1186/s13568-019-0763-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431307PMC
March 2019
1 Read

Experimental and bioinformatics study for production of L-asparaginase from Bacillus licheniformis: a promising enzyme for medical application.

AMB Express 2019 Mar 21;9(1):39. Epub 2019 Mar 21.

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, African Union Organization St. Abbassia, Cairo, 11566, Egypt.

A Bacillus licheniformis isolate with high L-asparaginase productivity was recovered upon screening two hundred soil samples. This isolate produces the two types of bacterial L-asparaginases, the intracellular type I and the extracellular type II. The catalytic activity of type II enzyme was much higher than that of type I and reached about 5. Read More

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http://dx.doi.org/10.1186/s13568-019-0751-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6428875PMC

Effects of biologically produced gold nanoparticles: toxicity assessment in different rat organs after intraperitoneal injection.

AMB Express 2019 Mar 19;9(1):38. Epub 2019 Mar 19.

Department of Medical Sciences, Shahrood Branch, Islamic Azad University, Shahrood, Iran.

Gold nanoparticles (GNPs) have different usage in the medical field. The plan of the present research was to evaluate the influence of the biologically produced GNPs on some rat organs. GNPs were produced using Fusarium oxysporum and their presence was confirmed using spectrophotometer, transmission electron microscope (TEM) and X-ray diffraction (XRD) analyses. Read More

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http://dx.doi.org/10.1186/s13568-019-0762-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424989PMC

Synergistic and protective effect of atorvastatin and amygdalin against histopathological and biochemical alterations in Sprague-Dawley rats with experimental endometriosis.

AMB Express 2019 Mar 19;9(1):37. Epub 2019 Mar 19.

Department of Reproductive Medicine, Maternal and Child Health Hospital of HuBei Province, Wuhan, 430072, Hubei, China.

The aim of the present study was to evaluate the protective effects of combined atorvastatin and amygdalin in a rat model of endometriosis. Tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), matrix metalloproteinase-2 (MMP-2) and MMP-9 levels in the peritoneal fluid were determined. The expression of TNF-α, IL-6, MMP-2, and MMP-9 mRNA, and the levels of lipid peroxidation, reduced glutathione (GSH), superoxide dismutase (SOD), catalase, and glutathione peroxidase (Gpx) were measured. Read More

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http://dx.doi.org/10.1186/s13568-019-0760-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424985PMC
March 2019
1 Read

Signal peptide replacement resulted in recombinant homologous expression of laccase Lcc8 in Coprinopsis cinerea.

AMB Express 2019 Mar 15;9(1):36. Epub 2019 Mar 15.

Institute of Food Chemistry and Food Biotechnology, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, 35392, Giessen, Germany.

Although the model agaricomycete Coprinopsis cinerea possess 17 different laccase genes, up to now only four C. cinerea laccases have been purified and characterized to some degree. By exchanging the nucleotide sequence of the deduced signal peptide of Lcc8 it was possible to homologously express lcc8 in C. Read More

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http://dx.doi.org/10.1186/s13568-019-0761-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420550PMC
March 2019
1 Read

Identification of the two-component guaiacol demethylase system from Rhodococcus rhodochrous and expression in Pseudomonas putida EM42 for guaiacol assimilation.

AMB Express 2019 Mar 11;9(1):34. Epub 2019 Mar 11.

Division of Applied Microbiology, Department of Chemistry, Lund University, P.O. Box 124, 221 00, Lund, Sweden.

A diversity of softwood lignin depolymerization processes yield guaiacol as the main low molecular weight product. This key aromatic compound can be utilized as a carbon source by several microbial species, most of which are Gram positive bacteria. Microbial degradation of guaiacol is known to proceed initially via demethylation to catechol, and this reaction is catalyzed by cytochrome P450 monooxygenases. Read More

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http://dx.doi.org/10.1186/s13568-019-0759-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411806PMC

In silico analysis as a strategy to identify candidate epitopes with human IgG reactivity to study Porphyromonas gingivalis virulence factors.

AMB Express 2019 Mar 11;9(1):35. Epub 2019 Mar 11.

Department of Health, Feira de Santana State University, Avenida Transnordestina s/n, Novo Horizonte, Feira de Santana, Bahia, CEP 44036-900, Brazil.

Porphyromonas gingivalis (Pg) is one of the main pathogens in chronic periodontitis (CP). Studies on the immunogenicity of its virulence factors may contribute to understanding the host response to infection. The present study aimed to use in silico analysis as a tool to identify epitopes from Lys-gingipain (Kgp) and neuraminidase virulence factors of the Pg ATCC 33277 strain. Read More

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http://dx.doi.org/10.1186/s13568-019-0757-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411804PMC
March 2019
1 Read

Effects of dietary supplementation of probiotic Enterococcus faecium on growth performance and gut microbiota in weaned piglets.

AMB Express 2019 Mar 1;9(1):33. Epub 2019 Mar 1.

Guangdong Provincial Key Laboratory of Animal Nutrition Control, Institute of Subtropical Animal Nutrition and Feed, College of Animal Science, South China Agricultural University, Guangzhou, 510640, China.

The adverse effects of antibiotics have attracted widespread attention, thus reducing the use of antibiotics in animal feed has become a very important issue in improving of the health of livestock. The effects of Enterococcus faecium (E. faecium) on growth performance and gut microbiota in weaned piglets were investigated in the present study. Read More

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http://dx.doi.org/10.1186/s13568-019-0755-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397275PMC
March 2019
2 Reads

Comparative analysis of the effect of Ca and Mg ions on antibacterial activity of lactic acid bacteria isolates and their associations depending on cultivation conditions.

AMB Express 2019 Feb 28;9(1):32. Epub 2019 Feb 28.

Department of Biochemistry, Microbiology and Biotechnology, Faculty of Biology, Yerevan State University, 1 Alex Manoogian Str., 0025, Yerevan, Armenia.

The effects of divalent cations of Ca and Mg on antibacterial activity of lactic acid bacteria (LAB) isolates, as well as their different associations were studied. Most LAB strains and associations revealed significant inhibitory effects in MRS against Gram-positive and Gram-negative test-organisms at different Ca and Mg concentrations (determined specifically for each LAB strain and each association). Some LAB strains and communities inhibited the growth of pathogenic test-organisms depending on both ions concentrations and cultivation conditions. Read More

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https://amb-express.springeropen.com/articles/10.1186/s13568
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http://dx.doi.org/10.1186/s13568-019-0758-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395461PMC
February 2019
5 Reads

Characteristics and influencing factors of amyloid fibers in S. mutans biofilm.

AMB Express 2019 Feb 28;9(1):31. Epub 2019 Feb 28.

Department of Preventive Dentistry, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, China.

There are signs that amyloid fibers exist in Streptococcus mutans biofilm recently. However, the characteristics of amyloid fibers and fibrillation influencing factors are unknown. In this study, we firstly used transmission electron microscopy (TEM) and atomic force microscopy (AFM) to observe the morphology of amyloid fibers in S. Read More

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http://dx.doi.org/10.1186/s13568-019-0753-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395465PMC
February 2019
3 Reads

Visual and rapid detection of Acinetobacter baumannii by a multiple cross displacement amplification combined with nanoparticles-based biosensor assay.

AMB Express 2019 Feb 26;9(1):30. Epub 2019 Feb 26.

Department of Respiratory and Critical Medical, Taihe Hospital, Hubei University of Medicine, Hubei, China.

The traditional microbiological methods used for detecting Acinetobacter baumannii were usually time-consuming and labor-intensive. Thus, we sought to establish a novel rapid detecting method for target pathogen. A set of multiple cross displacement amplification (MCDA) primers was designed to recognize 10 different regions of the pgaD gene, which was conservative and specific for the bacterium. Read More

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http://dx.doi.org/10.1186/s13568-019-0754-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391507PMC
February 2019

Effects of inoculation by arbuscular mycorrhizal fungi on the composition of the essential oil, plant growth, and lipoxygenase activity of Piper aduncum L.

AMB Express 2019 Feb 26;9(1):29. Epub 2019 Feb 26.

Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Pará, Belém, Pará, Brazil.

The aim of this study was to evaluate the changes in the production of secondary metabolites Piper aduncum seedlings were inoculated by spores of the arbuscular mycorrhizal fungi (AMF) Rhizophagus clarus and Claroideoglomus etunicatum. P. aduncum seedlings were inoculated by spores of R. Read More

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http://dx.doi.org/10.1186/s13568-019-0756-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391504PMC
February 2019

Biomethane production from sugar beet pulp under cocultivation with Clostridium cellulovorans and methanogens.

AMB Express 2019 Feb 18;9(1):28. Epub 2019 Feb 18.

Department of Life Sciences, Graduate School of Bioresources, Mie University, 1577 Kurimamachiya, Tsu, Mie, 514-8507, Japan.

This study was demonstrated with a coculture fermentation system using sugar beet pulp (SBP) as a carbon source combining the cellulose-degrading bacterium Clostridium cellulovorans with microbial flora of methane production (MFMP) for the direct conversion of cellulosic biomass to methane (CH). The MFMP was taken from a commercial methane fermentation plant and extremely complicated. Therefore, the MFMP was analyzed by a next-generation sequencing system and the microbiome was identified and classified based on several computer programs. Read More

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http://dx.doi.org/10.1186/s13568-019-0752-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379495PMC
February 2019

Ursolic acid inhibits colistin efflux and curtails colistin resistant Enterobacteriaceae.

AMB Express 2019 Feb 18;9(1):27. Epub 2019 Feb 18.

Center for Research on Infectious Diseases, SCBT, SASTRA Deemed University, Thanjavur, Tamil Nadu, India.

Colistin resistance in Enterobacteriaceae especially Klebsiella pneumoniae and Escherichia coli is driving the evolution of pan drug resistant strains. Screening a library of 13 plant nutraceuticals led to the identification of acetyl shikonin and ursolic acid, which exhibited synergy with colistin against extremely drug resistant (XDR) clinical strains of E. coli (U3790) and K. Read More

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http://dx.doi.org/10.1186/s13568-019-0750-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379497PMC
February 2019
1 Read

Dietary supplementation with fermented Mao-tai lees beneficially affects gut microbiota structure and function in pigs.

AMB Express 2019 Feb 18;9(1):26. Epub 2019 Feb 18.

Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, 410125, China.

Gut microbiota positively contribute to livestock nutrition and metabolism. The manipulation of these microbes may improve animal health. Some feed additives improve livestock health and metabolism by regulating gut microbiota composition and activity. Read More

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http://dx.doi.org/10.1186/s13568-019-0747-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379501PMC
February 2019
1 Read

Enhancement of ascomycin production via a combination of atmospheric and room temperature plasma mutagenesis in Streptomyces hygroscopicus and medium optimization.

AMB Express 2019 Feb 18;9(1):25. Epub 2019 Feb 18.

Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai, 201203, China.

Ascomycin, a key intermediate for chemical synthesis of immunosuppressive drug pimecrolimus, is produced by Streptomyces hygroscopicus var. ascomyceticus. In order to improve the strain production, the original S. Read More

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http://dx.doi.org/10.1186/s13568-019-0749-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379505PMC
February 2019

A thermostable laccase from Thermus sp. 2.9 and its potential for delignification of Eucalyptus biomass.

AMB Express 2019 Feb 12;9(1):24. Epub 2019 Feb 12.

Instituto de Microbiología y Zoología Agrícola, Instituto Nacional de Tecnología Agropecuaria (INTA), Nicolás Repetto y De los Reseros s/n., 1686, Hurlingham, Buenos Aires, Argentina.

Laccases are multicopper oxidases that are being studied for their potential application in pretreatment strategies of lignocellulosic feedstocks for bioethanol production. Here, we report the expression and characterization of a predicted laccase (LAC_2.9) from the thermophilic bacterial strain Thermus sp. Read More

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http://dx.doi.org/10.1186/s13568-019-0748-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6372703PMC
February 2019

Pseudotyping retrovirus like particles vaccine candidates with Hepatitis C virus envelope protein E2 requires the cellular expression of CD81.

AMB Express 2019 Feb 7;9(1):22. Epub 2019 Feb 7.

iBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2780-901, Oeiras, Portugal.

Hepatitis C virus (HCV) infects 3% of world population being responsible for nearly half a million deaths annually urging the need for a prophylactic vaccine. Retrovirus like particles are commonly used scaffolds for antigens presentation being the core of diverse vaccine candidates. The immunogenicity of host proteins naturally incorporated in retrovirus was hypothesized to impact the performance of retrovirus based vaccines. Read More

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http://dx.doi.org/10.1186/s13568-019-0741-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6367494PMC
February 2019
4 Reads

Characterization of the protein fraction of the extracellular polymeric substances of three anaerobic granular sludges.

AMB Express 2019 Feb 7;9(1):23. Epub 2019 Feb 7.

Anaerobic Bioprocesses Group, Energy, Mining and Environment Research Centre, National Research Council Canada, 6100 Royalmount Avenue, Montreal, H4P 2R2, Canada.

Extracellular polymeric substances (EPS) play major roles in the efficacy of biofilms such as anaerobic granules, ranging from structural stability to more specific functions. The EPS of three granular anaerobic sludges of different origins were studied and compared. Particularly, the peptides from the protein fraction were identified by mass spectrometry. Read More

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http://dx.doi.org/10.1186/s13568-019-0746-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6367495PMC
February 2019
1 Read

In silico genome analysis reveals the metabolic versatility and biotechnology potential of a halotorelant phthalic acid esters degrading Gordonia alkanivorans strain YC-RL2.

AMB Express 2019 Feb 4;9(1):21. Epub 2019 Feb 4.

Biological Laboratory, Department of Biology, Graduate School of Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.

Members of genus Gordonia are known to degrade various xenobitics and produce secondary metabolites. The genome of a halotorelant phthalic acid ester (PAEs) degrading actinobacterium Gordonia alkanivorans strain YC-RL2 was sequenced using Biosciences RS II platform and Single Molecular Real-Time (SMRT) technology. The reads were assembled de novo by hierarchical genome assembly process (HGAP) algorithm version 2. Read More

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http://dx.doi.org/10.1186/s13568-019-0733-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362181PMC
February 2019
1 Read

Antimicrobial potential of toothpaste formulated from extracts of Syzygium aromaticum, Dennettia tripetala and Jatropha curcas latex against some oral pathogenic microorganisms.

AMB Express 2019 Feb 4;9(1):20. Epub 2019 Feb 4.

Department of Microbiology, The Federal University of Technology, PMB 704, Akure, Nigeria.

The need for a broad spectrum antimicrobial mouthwash is highly desirable to reduce, control and prevent various types of dental diseases. Hence, research into the production of herbal toothpaste to suppress the incidence of dental diseases is pertinent. The present study formulated herbal toothpastes from edible and medicinal plants namely; Syzygium aromaticum, Dennettia tripetala, and Jatropha curcas latex. Read More

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http://dx.doi.org/10.1186/s13568-019-0744-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362185PMC
February 2019
1 Read

Isolation and biochemical characterization of a metagenome-derived 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase gene from subtropical marine mangrove wetland sediments.

AMB Express 2019 Feb 4;9(1):19. Epub 2019 Feb 4.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue East Road, Nanning, 530004, Guangxi, People's Republic of China.

3-Deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHPS) is a key rate-limiting enzyme in aromatic amino acid anabolism. A new I-type DAHPS gene (aro1A) was identified in a metagenomic library from subtropical marine mangrove sediment. The gene encoded a polypeptide composed of 272 amino acids and had a maximum similarity of 52. Read More

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https://amb-express.springeropen.com/articles/10.1186/s13568
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http://dx.doi.org/10.1186/s13568-019-0742-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362186PMC
February 2019
12 Reads

Coupled changes of bacterial community and function in the gut of mud crab (Scylla Paramamosain) in response to Baimang disease.

AMB Express 2019 Feb 2;9(1):18. Epub 2019 Feb 2.

Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 309 of Building Keyan, 231 Xingang Xi Road, Guangzhou, China.

Increasing evidence has revealed a close association between intestinal bacterial community and hosts health. However, it is unclear whether and what extend Baimang disease alters the intestinal microbiota in mud crab (Scylla paramamosain). Here, we conducted intestinal contents Illumina sequencing of healthy and Baimang diseased mud crab (S. Read More

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http://dx.doi.org/10.1186/s13568-019-0745-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359999PMC
February 2019

Mixed alcoholic fermentation of Schizosaccharomyces pombe and Lachancea thermotolerans and its influence on mannose-containing polysaccharides wine Composition.

AMB Express 2019 Feb 2;9(1):17. Epub 2019 Feb 2.

Department of Chemistry and Food Technology, Polytechnic University of Madrid, Ciudad Universitaria S/N, 28040, Madrid, Spain.

This study researched the winemaking performance of new biotechnology involving the cooperation of Lachancea and Schizosaccharomyces genera in the production of wine. In all fermentations where Lachancea thermotolerans was involved, higher lactic acid concentrations appeared, while all fermentations where Schizosaccharomyces pombe was involved, lower levels in malic acid concentration took place. The sensorial properties of the final wines varied accordingly. Read More

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http://dx.doi.org/10.1186/s13568-019-0738-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360000PMC
February 2019
1 Read

Design and activity study of a melittin-thanatin hybrid peptide.

AMB Express 2019 Jan 30;9(1):14. Epub 2019 Jan 30.

College of Life Sciences And Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, Zhejiang, China.

The unique antimicrobial mechanism of antimicrobials make them a promising substitute for antibiotics for fighting drug-resistant bacteria. Both melittin and thanatin have antimicrobial bioactivity. However, thanatin does not inhibit the growth of Staphylococcus aureus. Read More

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http://dx.doi.org/10.1186/s13568-019-0739-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353975PMC
January 2019

Co-culture of Bacillus coagulans and Candida utilis efficiently treats Lactobacillus fermentation wastewater.

AMB Express 2019 Jan 30;9(1):15. Epub 2019 Jan 30.

College of Tropical Agriculture and Human Resources, University of Hawaii, Honolulu, HI, 96822, USA.

Co-culture of Bacillus coagulans and Candida utilis was firstly investigated in the efficient treatment of Lactobacillus fermentation wastewater (LFW) containing total organic carbon (TOC) of 22.0 g/L and total nitrogen (TN) of 2.4 g/L. Read More

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https://amb-express.springeropen.com/articles/10.1186/s13568
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http://dx.doi.org/10.1186/s13568-019-0743-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353979PMC
January 2019
9 Reads

Microbial hexuronate catabolism in biotechnology.

AMB Express 2019 Jan 30;9(1):16. Epub 2019 Jan 30.

VTT Technical Research Centre of Finland Ltd, PO box 1000, 02044 VTT, Espoo, Finland.

The most abundant hexuronate in plant biomass is D-galacturonate. D-Galacturonate is the main constituent of pectin. Pectin-rich biomass is abundantly available as sugar beet pulp or citrus processing waste and is currently mainly used as cattle feed. Read More

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http://dx.doi.org/10.1186/s13568-019-0737-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353982PMC
January 2019

Synergistic effect of acetyl xylan esterase from Talaromyces leycettanus JCM12802 and xylanase from Neocallimastix patriciarum achieved by introducing carbohydrate-binding module-1.

AMB Express 2019 Jan 29;9(1):13. Epub 2019 Jan 29.

Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing, 100081, People's Republic of China.

Wheat bran is an effective raw material for preparation xylooligosaccharides; however, current research mainly focuses on alkali extraction and enzymatic hydrolysis methods. Since ester bonds are destroyed during the alkali extraction process, xylanase and arabinofuranosidase are mainly used to hydrolyze xylooligosaccharides. However, alkali extraction costs are very high, and the method also causes pollution. Read More

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http://dx.doi.org/10.1186/s13568-019-0740-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351639PMC
January 2019
3 Reads

New bioactive metabolites from the elicited marine sponge-derived bacterium Actinokineospora spheciospongiae sp. nov.

AMB Express 2019 Jan 24;9(1):12. Epub 2019 Jan 24.

Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, 61519, Egypt.

Several approaches have been dedicated to activate the cryptic gene clusters in the genomes of actinomycetes for the targeted discovery of new fascinating biomedical lead structures. In the current study, N-acetylglucosamine was used to maximize the chemical diversity of sponge-derived actinomycete Actinokineospora spheciospongiae sp. nov. Read More

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http://dx.doi.org/10.1186/s13568-018-0730-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6345950PMC
January 2019
1 Read

Development of a loop-mediated isothermal amplification coupled lateral flow dipstick targeting erm(41) for detection of Mycobacterium abscessus and Mycobacterium massiliense.

AMB Express 2019 Jan 23;9(1):11. Epub 2019 Jan 23.

National Tuberculosis Reference Laboratory, Chinese Center for Disease Control and Prevention, Changping, Changbai Road 155, Beijing, 102206, China.

Mycobacterium abscessus (M. abscessus) and Mycobacterium massiliense (M. massiliense) are major pathogens that cause post-surgical wound infection and chronic pulmonary disease. Read More

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http://dx.doi.org/10.1186/s13568-019-0734-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344564PMC
January 2019
6 Reads

Continuous degradation of phenanthrene in cloud point system by reuse of Sphingomonas polyaromaticivorans cells.

AMB Express 2019 Jan 19;9(1). Epub 2019 Jan 19.

State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangdong, 510070, China.

Extractive biodegradation of phenanthrene by Sphingomonas polyaromaticivorans was previously carried out in cloud point system (CPS). In this study, we explored the possibility of further increasing the efficiency of the culture by repeatedly reusing cells and the system for biodegradation. Three different recycling strategies were adopted. Read More

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http://dx.doi.org/10.1186/s13568-019-0736-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339633PMC
January 2019
1 Read

Screening and identification of Lactic acid bacteria from Ya'an pickle water to effectively remove Pb.

AMB Express 2019 Jan 19;9(1):10. Epub 2019 Jan 19.

College of Food Science, Sichuan Agricultural University, Ya'an, 625014, Sichuan, People's Republic of China.

Heavy metal lead, which enters the human body through food intake, endangers human health. Microbe has the ability of adsorbing heavy metal, among which lactic acid bacteria are promising microbes to adsorb and remove Pb. The purpose of this study was to screen lactic acid bacteria from Ya'an pickle water to effectively remove Pb. Read More

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http://dx.doi.org/10.1186/s13568-018-0724-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339634PMC
January 2019
11 Reads

Characterization of GH2 and GH42 β-galactosidases derived from bifidobacterial infant isolates.

AMB Express 2019 Jan 19;9(1). Epub 2019 Jan 19.

APC Microbiome Ireland, University College Cork, Western Road, Cork, Ireland.

Bifidobacteria are among the first and most abundant bacterial colonizers of the gastrointestinal tract of (breast-fed) healthy infants. Their success of colonising the infant gut is believed to be, at least partly, due to their ability to metabolize available carbon sources by means of secreted or intracellular glycosyl hydrolases (GHs). Among these, β-galactosidases are particularly relevant as they allow bifidobacteria to grow on β-galactosyl-linked saccharidic substrates, which are present in copious amounts in the milk-based diet of their infant host (e. Read More

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http://dx.doi.org/10.1186/s13568-019-0735-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339635PMC
January 2019
1 Read

Expression in eukaryotic cells and purification of synthetic gene encoding enterocin P: a bacteriocin with broad antimicrobial spectrum.

AMB Express 2019 Jan 7;9(1). Epub 2019 Jan 7.

Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Due to the emergence of multidrug-resistant bacteria, treatment options for infectious diseases are decreasing. Bacteriocins are small antimicrobial peptides produced by numerous bacteria that offer alternative therapeutic strategies to combat multidrug-resistant bacterial infections. We evaluated the cloning, functional expression, and antimicrobial activities of enterocin P (EntP), a class II bacteriocin member, in Chinese hamster ovary (CHO) cells. Read More

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https://amb-express.springeropen.com/articles/10.1186/s13568
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http://dx.doi.org/10.1186/s13568-018-0729-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323060PMC
January 2019
17 Reads

Rhamnolipid production by a gamma ray-induced Pseudomonas aeruginosa mutant under solid state fermentation.

AMB Express 2019 Jan 8;9(1). Epub 2019 Jan 8.

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Organization of African Unity St., Abbassia, POB: 11566, Cairo, Egypt.

Solid-state fermentation has a special advantage of preventing the foaming problem that obstructs submerged fermentation processes for rhamnolipid production. In the present work, a 50:50 mixture of sugarcane bagasse and sunflower seed meal was selected as the optimum substrate for rhamnolipid production using a Pseudomonas aeruginosa mutant 15GR and an impregnating solution including 5% v/v glycerol. Using Box-Behnken design, the optimum fermentation conditions were found to be an inoculum size 1% v/v, temperature 30 °C and unlike other studies, pH 8. Read More

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http://dx.doi.org/10.1186/s13568-018-0732-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6325051PMC
January 2019

Ability of different edible fungi to degrade crop straw.

AMB Express 2019 Jan 7;9(1). Epub 2019 Jan 7.

College of Agronomy and Resources Environment, Tianjin Agricultural University, Tianjin, 300384, China.

Extracellular enzymes play an important role in the growth and development of edible fungi. Extracellular enzyme activities have also become an important object of measurement. In this study, Agaricus brunnescens Peck, Coprinus comatus, and Pleurotus ostreatus were compared in terms of their enzyme production in liquid-and solid-state fermentation. Read More

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https://amb-express.springeropen.com/articles/10.1186/s13568
Publisher Site
http://dx.doi.org/10.1186/s13568-018-0731-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323044PMC
January 2019
8 Reads