1,211 results match your criteria Amb Express[Journal]


Maslinic acid induces anticancer effects in human neuroblastoma cells mediated via apoptosis induction and caspase activation, inhibition of cell migration and invasion and targeting MAPK/ERK signaling pathway.

AMB Express 2020 Jun 1;10(1):104. Epub 2020 Jun 1.

Department of Pediatric Endocrinology and Genetic Metabolic Diseases, Qingdao Women and Children's Hospital, Qingdao, 266000, Shandong, China.

Maslinic acid is an active member of pentacyclic triterpenes predominantly found in dietary plants including hawthorn berries and olive fruit skins. It has been reported to show immense pharmacological and biological importance including anticancer property. This research was initiated to explore the anticancer potential of maslinic acid against human neuroblastoma. Read More

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http://dx.doi.org/10.1186/s13568-020-01035-1DOI Listing

Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2.

AMB Express 2020 Jun 1;10(1):103. Epub 2020 Jun 1.

MOE Joint International Research Laboratory of Animal Health and Food Safety, Key Lab of Animal Bacteriology of Ministry of Agriculture, OIE Reference Lab for Swine Streptococcosis, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.

Streptococcus suis, a major emerging pathogen in swine and humans, expresses immunoglobulin G (IgG)-binding proteins (IBPs), which contribute to the ability of organism to evasion of host immune system. The objective of this study was to identify novel pig IgG (pIgG) and human IgG (hIgG)-binding proteins and characterize the binding regions of enolase from Streptococcus suis serotype 2 (S. suis 2). Read More

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http://dx.doi.org/10.1186/s13568-020-01042-2DOI Listing

Dynamics of Clostridium genus and hard-cheese spoiling Clostridium species in anaerobic digesters treating agricultural biomass.

AMB Express 2020 Jun 1;10(1):102. Epub 2020 Jun 1.

Department for Sustainable Food Process - DiSTAS, Università Cattolica del Sacro Cuore, Via Emilia Parmense, 84, 29122, Piacenza, Italy.

Biogas plants are a widespread renewable energy technology. However, the use of digestate for agronomic purposes has often been a matter of concern. It is controversial whether biogas plants might harbor some pathogenic clostridial species, which represent a biological risk. Read More

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http://dx.doi.org/10.1186/s13568-020-01040-4DOI Listing

Identification of a species-specific aminotransferase in Pediococcus acidilactici capable of forming α-aminobutyrate.

AMB Express 2020 May 29;10(1):100. Epub 2020 May 29.

Agroscope, Schwarzenburgstrasse 161, 3003, Bern, Switzerland.

During cheese ripening, the bacterial strain Pediococcus acidilactici FAM18098 produces the non-proteinogenic amino acid, α-aminobutyrate (AABA). The metabolic processes that lead to the biosynthesis of this compound are unknown. In this study, 10 P. Read More

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http://dx.doi.org/10.1186/s13568-020-01034-2DOI Listing

Probiotic Bifidobacterium lactis V9 attenuates hepatic steatosis and inflammation in rats with non-alcoholic fatty liver disease.

AMB Express 2020 May 29;10(1):101. Epub 2020 May 29.

College of Life Science, Inner Mongolia Agricultural University, Hohhot, 010018, People's Republic of China.

Both steatosis and inflammation are key pathological events in the progression of non-alcoholic fatty liver disease (NAFLD). Probiotics are beneficial for the prevention and treatment of NAFLD. Bifidobacterium animalis subsp. Read More

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http://dx.doi.org/10.1186/s13568-020-01038-yDOI Listing

Prevalence, the antibiogram and the frequency of virulence genes of the most predominant bacterial pathogens incriminated in calf pneumonia.

AMB Express 2020 May 29;10(1):99. Epub 2020 May 29.

Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.

The purpose of this study was to investigate the prevalence, antibiotic resistance and certain virulence genes of the most predominant bacterial pathogens causing BRD. A total of 225 calves; 55 apparently healthy and 170 diseased; were sampled. Bacteriological examination, antimicrobial susceptibility testing and PCR based detection of some virulence genes were performed. Read More

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http://dx.doi.org/10.1186/s13568-020-01037-zDOI Listing

Effect of the selection pressure of vaccine antibodies on evolution of H9N2 avian influenza virus in chickens.

AMB Express 2020 May 27;10(1):98. Epub 2020 May 27.

College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, China.

H9N2 avian influenza virus has spread worldwide, and vaccination with an inactivated virus is currently the major prevention method in China. To further understand the effect of the selection pressure from antibodies on the evolution of H9N2 avian influenza virus, F/98 (A/Chicken/Shanghai/F/98), which is the vaccine representative of H9N2 avian influenza virus in East China, was used for serial passaging for 20 generations in chickens with and without vaccination. After plaque purification from trachea and lung tissues, 390 quasispecies were obtained. Read More

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http://dx.doi.org/10.1186/s13568-020-01036-0DOI Listing

Characterization of FsXEG12A from the cellulose-degrading ectosymbiotic fungus Fusarium spp. strain EI cultured by the ambrosia beetle.

AMB Express 2020 May 24;10(1):96. Epub 2020 May 24.

Faculty of Agriculture, Meijo University, Nagoya, Aichi, 468-8502, Japan.

Despite the threat of Fusarium dieback posed due to ambrosia fungi cultured by ambrosia beetles such as Euwallacea spp., the wood-degradation mechanisms utilized by ambrosia fungi are not fully understood. In this study, we analyzed the 16S rRNA and 18S rRNA genes of the microbial community from the Ficus tree tunnel excavated by Euwallacea interjectus and isolated the cellulose-degrading fungus, Fusarium spp. Read More

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http://dx.doi.org/10.1186/s13568-020-01030-6DOI Listing

Metabolic enzyme clustering by coiled coils improves the biosynthesis of resveratrol and mevalonate.

AMB Express 2020 May 24;10(1):97. Epub 2020 May 24.

Department of Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19, 1000, Ljubljana, Slovenia.

The clustering of biosynthetic enzymes is used in nature to channel reaction products and increase the yield of compounds produced by multiple reaction steps. The coupling of multiple enzymes has been shown to increase the biosynthetic product yield. Different clustering strategies have particular advantages as the spatial organization of multiple enzymes creates biocatalytic cascades with a higher efficiency of biochemical reaction. Read More

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http://dx.doi.org/10.1186/s13568-020-01031-5DOI Listing

Development of diagnostic tools for IBDV detection using plants as bioreactors.

AMB Express 2020 May 20;10(1):95. Epub 2020 May 20.

Instituto de Agrobiotecnología y Biología Molecular (IABIMO), Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), De los Reseros y Nicolás Repetto s/n, 1686, Hurlingham, Buenos Aires, Argentina.

Infectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds, thus causing important economic losses in the poultry industry. Multimeric particles with different architectures based on the capsid protein VP2 have been widely produced for different purposes. We hereby show the production and easy recovery of IBDV subviral particles (SVP) from transiently transformed Nicotiana benthamiana. Read More

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http://dx.doi.org/10.1186/s13568-020-01029-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239984PMC

Anti-glaucoma potential of hesperidin in experimental glaucoma induced rats.

AMB Express 2020 May 18;10(1):94. Epub 2020 May 18.

Department of Ophthalmology, The First Affiliated Hospital of Kangda College of Nanjing Medical University, Xuzhou Medical University Affiliated Hospital of Lianyungang, The First People's Hospital of Lianyungang, No. 6 Zhenhua East Road, Haizhou District, Lianyungang, 222002, China.

Glaucoma is well-known clinical eye conditions that damage the optic nerve due to abnormal pressure conditions in eye. Hesperidin is well-known glycoside widely present in the citrus fruits, and its aglycone form is known as hesperetin. Hesperidin is major flavone found in orange fruits. Read More

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http://dx.doi.org/10.1186/s13568-020-01027-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235152PMC

The role of environmental factors to transmission of SARS-CoV-2 (COVID-19).

AMB Express 2020 May 15;10(1):92. Epub 2020 May 15.

Environmental Science and Technology Research Center, Department of Environmental Health Engineering, School of Public Health, Shahid Sadoughi University of Medical Sciences, Pardis Campus, Gomnam Blv, Alem Squre, Yazd, Iran.

The current outbreak of the novel coronavirus disease 2019 (COVID-19) in more than 250 countries has become a serious threat to the health of people around the world. Human-to-human transmission of the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) occurs most often when people are in the incubation stage of the disease or are carriers and have no symptoms. Therefore, in this study, was discussed the role of environmental factors and conditions such as temperature, humidity, wind speed as well as food, water and sewage, air, insects, inanimate surfaces, and hands in COVID-19 transmission. Read More

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http://dx.doi.org/10.1186/s13568-020-01028-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226715PMC

Rich production media as a platform for CHO cell line development.

AMB Express 2020 May 15;10(1):93. Epub 2020 May 15.

Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea.

Recent cell culture media for mammalian cells can be abundantly formulated with nutrients supporting production, but such media can be limited to use in host cell culture, transfection, cell cloning, and cell growth under the low cell density conditions. In many cases, appropriate platform media are used for cell line development, and then replaced with rich media for production. In this study, we demonstrate rich chemically defined media for Chinese hamster ovary (CHO) cells that are suitable as basal media both for cell line development and for final production of culture process. Read More

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http://dx.doi.org/10.1186/s13568-020-01025-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229095PMC

Characterization and antimicrobial activity of lectins purified from three Egyptian leguminous seeds.

AMB Express 2020 May 15;10(1):90. Epub 2020 May 15.

Botany, Ain Shams University, Cairo, Egypt.

Lectins are carbohydrate-binding proteins that play vital roles in many biological processes. In this study, lectins from three Egyptian cultivars (fava bean, lentil, and pea) were isolated by precipitation with different concentrations of ammonium sulfate. The purification process was performed by affinity chromatography using mannose agarose. Read More

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http://dx.doi.org/10.1186/s13568-020-01024-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229064PMC

Isolation of novel Lactobacillus with lipolytic activity from the vinasse and their preliminary potential using as probiotics.

AMB Express 2020 May 15;10(1):91. Epub 2020 May 15.

Key Lab of Dairy Biotechnology and Safety Control, College of Food Science and Technology, Yangzhou University, Yangzhou, 225127, Jiangsu, China.

Lactobacillus casei f1, L. paracasei f2 and L. paracasei f3 with lipolytic activity were isolated and identified from vinasses according to the morphological-physiological properties detection and 16S rDNA analysis. Read More

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http://dx.doi.org/10.1186/s13568-020-01026-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229107PMC

Exploration of the safe water content and activity control points for medicinal and edible lotus seeds from mildew.

AMB Express 2020 May 12;10(1):89. Epub 2020 May 12.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, China.

Affected by the inner properties and the external environmental conditions, medicinal and edible lotus seeds are susceptible to mildew with fungal infection under suitable temperature and humidity conditions, leading to the production and contamination of various mycotoxins, along with threats to its quality and safety. In this study, the changes of water content (C) and water activity (A) of lotus seeds stored at 25 °C and different relative humidity conditions, as well as the correlation between them and mildew of this edible and medicinal material were studied, aiming to explore the safe C and A control points for screening out the suitable storage conditions from mildew. Blank (without fungal conidia) and experimental (artificially added with Aspergillus flavus conidia) groups of lotus seeds were stored at 25 °C and relative humidity of 40%, 50%, 60% and 70% for about 30 days, respectively. Read More

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http://dx.doi.org/10.1186/s13568-020-01019-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218039PMC

Biotransformation of 5-hydroxymethylfurfural into 2,5-dihydroxymethylfuran by Ganoderma sessile and toxicological assessment of both compounds.

AMB Express 2020 May 11;10(1):88. Epub 2020 May 11.

Department of Bioengineering, Zhuhai Campus of Zunyi Medical University, Zhuhai, 519041, Guangdong, China.

Biotransformation has the advantages of low cost and environmental protection and is a preferred method for production of compounds. At present, most 2,5-dihydroxymethylfuran (DHMF) is synthesized by chemical methods. In this study, 12. Read More

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http://dx.doi.org/10.1186/s13568-020-01023-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7214591PMC

Cinnamtannin A2 protects the renal injury by attenuates the altered expression of kidney injury molecule 1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) expression in 5/6 nephrectomized rat model.

AMB Express 2020 May 8;10(1):87. Epub 2020 May 8.

Department of Nephrology, The third Hospital of Jilin University, No 126 Xiantai Street, Changchun, 130033, Jilin, People's Republic of China.

Present investigation determines the protective effect of Cinnamtannin A2 against chronic renal failure (CRF). 5/6 nephrectomized rat model was used to induced CRF by removing the kidneys and rats were treated with Cinnamtannin A2 10 mg/kg, i.p. Read More

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http://dx.doi.org/10.1186/s13568-020-01022-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7210374PMC

Survival strategy of Pseudomonas aeruginosa on the nanopillar topography of dragonfly (Pantala flavescens) wing.

AMB Express 2020 May 6;10(1):85. Epub 2020 May 6.

Department of Biotechnology, PSG College of Technology, Coimbatore, TamilNadu, India.

Discovery of nanopillars on the surface of the insect wings had led to the understanding of its bactericidal property. Nanopillar topography is deterrent to only those bacteria that are attached, or in close contact with the nanopillars. The present study investigated the variation in the viability of Pseudomonas aeruginosa strains PAO1 (virulent) and ATCC 9027 (avirulent) on the wing surface of dragonfly (Pantala flavescens). Read More

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http://dx.doi.org/10.1186/s13568-020-01021-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7203277PMC

High-yield grass Pennisetum sinese Roxb plantation and organic manure alter bacterial and fungal communities structure in an ecological agriculture farm.

AMB Express 2020 May 6;10(1):86. Epub 2020 May 6.

Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, Nanchong, 637002, China.

Pennisetum sinese Roxb (P. sinese) is an efficient and economic energy crop for its high productivity, and has been well studied in its application in phytoremediation and fodder production. However, little is known about how P. Read More

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http://dx.doi.org/10.1186/s13568-020-01018-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7203373PMC

Raman and FT-IR Spectroscopy investigation the cellulose structural differences from bacteria Gluconacetobacter sucrofermentans during the different regimes of cultivation on a molasses media.

AMB Express 2020 May 3;10(1):84. Epub 2020 May 3.

National Research Mordovia State University, Saransk, Russia.

Raman and Fourier Transform Infrared (FT-IR) spectroscopy was used for investigation of structural differences of bacterial celluloses (BC), obtained by cultivation native and immobilized cells of Gluconacetobacter sucrofermentans during static and dynamic regimes of cultivation on a molasses media. It was found that the Raman and FT-IR spectra could characterized the groups of the cellulose molecules. The culturing bacterial cellulose in the presence of results in an increase of crystalline and it increased during cultivated on a molasses media with the addition of 1. Read More

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http://dx.doi.org/10.1186/s13568-020-01020-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7196602PMC

Identification of key genes and pathways in syphilis combined with diabetes: a bioinformatics study.

AMB Express 2020 Apr 27;10(1):83. Epub 2020 Apr 27.

Department of Clinical Laboratory, The First Affiliated Hospital of University of South China, No. 69, Chuanshan Road, Shigu District, Hengyang City, 421000, Hunan, China.

We noticed that syphilis patients seem to be more susceptible to diabetes and the lesions often involve the kidneys, but the pathogenesis is not yet completely understood. In this study, microarray analysis was performed to investigate the dysregulated expressed genes (DEGs) in rabbit model of syphilis combined with diabetes. A total of 1045 genes were identified to be significantly differentially expressed, among which 571 were up-regulated and 474 were down-regulated (≥ 2. Read More

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http://dx.doi.org/10.1186/s13568-020-01009-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7186291PMC

Synthesis, characterization and assessment of anti-quorum sensing activity of copper(II)-ciprofloxacin complex against Pseudomonas aeruginosa PAO1.

AMB Express 2020 Apr 24;10(1):82. Epub 2020 Apr 24.

Department of Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.

Quorum sensing (QS) inhibition by metal-antibiotic complexes is a promising strategy for the management and control of multidrug resistant Pseudomonas aeruginosa infections. We investigated the anti-quorum sensing activity of sub-minimum inhibitory concentration (sub-MIC) of copper(II) sulfate pentahydrate-ciprofloxacin (Cu-CIP) complex and free ciprofloxacin (free-CIP) against P. aeruginosa PAO1. Read More

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http://dx.doi.org/10.1186/s13568-020-01017-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182649PMC

Exploration of isoxanthohumol bioconversion from spent hops into 8-prenylnaringenin using resting cells of Eubacterium limosum.

AMB Express 2020 Apr 24;10(1):79. Epub 2020 Apr 24.

AVECOM NV, Ind Weg 122P, 9032, Wondelgem, Belgium.

Hops is an almost unique source of the potent phytoestrogen 8-prenylnaringenin (8-PN). As hops contain only low levels of 8-PN, synthesis may be more attractive than extraction. A strain of the Gram-positive Eubacterium limosum was isolated previously for 8-PN production from more abundant precursor isoxanthohumol (IX) from hops. Read More

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http://dx.doi.org/10.1186/s13568-020-01015-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182650PMC

Therapeutic effect of GLP-1 engineered strain on mice model of Alzheimer's disease and Parkinson's disease.

AMB Express 2020 Apr 24;10(1):80. Epub 2020 Apr 24.

Department of Neurology, The First Affiliated Hospital of Nanchang University, Nanchang, China.

Alzheimer's disease (AD) and Parkinson's disease (PD) are neurodegenerative diseases (NDD) characterized by progressive degeneration of the central nervous system, and few medications are available to halt the progression of AD and PD. In the present study, an engineered strain MG136-pMG36e-GLP-1 was used to evaluate its neuroprotective effect on AD and PD mice, via the probiotics effects of Lactococcus lactis MG1363 and the constantly produced Glucagon-like peptide-1 (GLP-1) by the engineered strain. Our results indicated that oral administration of MG136-pMG36e-GLP-1 significantly reduced lipopolysaccharide (LPS)-induced memory impairment and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced motor dysfunction through the toll-like receptor4 (TLR4)/nuclear factor-kappa B (NFκB) and protein kinase B (AKT)/Glycogen synthase kinase-3β (GSK3β) signaling pathway. Read More

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http://dx.doi.org/10.1186/s13568-020-01014-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182653PMC

Development of a standardized method to evaluate the protective efficiency of cosmetic packaging against microbial contamination.

AMB Express 2020 Apr 24;10(1):81. Epub 2020 Apr 24.

Laboratory of Microbiology Signals and Microenvironment (LMSM EA 4312), Normandie Univ, Univ. Rouen, 55 rue Saint-Germain, 27000, Evreux, France.

Doubts surrounding the potential adverse effects of antimicrobial preservatives have modified the demand of consumers, who increasingly insist on the production of low-level and even preservative-free cosmetics. Protection of the product against microbial contamination is therefore focused on the packaging. This has prompted the emergence of a highly diverse array of so-called "protective", "overprotective", and "barrier" packaging. Read More

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http://dx.doi.org/10.1186/s13568-020-01016-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182652PMC

Identification of genes involved in xylose metabolism of Meyerozyma guilliermondii and their genetic engineering for increased xylitol production.

AMB Express 2020 Apr 20;10(1):78. Epub 2020 Apr 20.

University of Applied Sciences Upper Austria, Stelzhamerstraße 23, 4600, Wels, Austria.

Meyerozyma guilliermondii, a non-conventional yeast that naturally assimilates xylose, is considered as a candidate for biotechnological production of the sugar alternative xylitol. Because the genes of the xylose metabolism were yet unknown, all efforts published so far to increase the xylitol yield of this yeast are limited to fermentation optimization. Hence, this study aimed to genetically engineer this organism for the first time with the objective to increase xylitol production. Read More

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http://dx.doi.org/10.1186/s13568-020-01012-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7171046PMC

Analysis and effect of conventional flasks in shaking culture of Escherichia coli.

AMB Express 2020 Apr 19;10(1):77. Epub 2020 Apr 19.

Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan.

The circulation direct monitoring and sampling system (CDMSS) is used as a monitoring device for CO and O concentrations of bypass type in shake-culture flask. The CDMSS could measure ka, an index for evaluating the performance of aerobic culture incubators, and k, an indicator of the degree of CO ventilation in the flask gas phase. We observed that cylindrical flasks provided a different culture environment, yielded a much higher k than the Erlenmeyer and Sakaguchi flasks, and yielded ka equivalent to that by Erlenmeyer flask by setting the ring-type baffle appropriately. Read More

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http://dx.doi.org/10.1186/s13568-020-01013-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7167391PMC

The regulatory effect of Lactobacillus rhamnosus GG on T lymphocyte and the development of intestinal villi in piglets of different periods.

AMB Express 2020 Apr 17;10(1):76. Epub 2020 Apr 17.

College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, 2888 Xincheng Street, Changchun, 130118, China.

The maturation and development of T cells were not completed until T cells were selected in thymus. It was not until the early 1960s that j.f. Read More

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http://dx.doi.org/10.1186/s13568-020-00980-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7165236PMC

Comparison of the intensity of biofilm formation by Listeria monocytogenes using classical culture-based method and digital droplet PCR.

AMB Express 2020 Apr 17;10(1):75. Epub 2020 Apr 17.

Department of Microbiology, Nicolaus Copernicus University in Toruń, Collegium Medicum of L. Rydygier in Bydgoszcz, Bydgoszcz, Poland.

Listeria monocytogenes is a Gram-positive bacterium, commonly found in food, water or sewage. This microorganism is capable of forming biofilm on different surfaces such as steel, glass, polypropylene etc. Recently an increase in cases of listeriosis has been noted, making L. Read More

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http://dx.doi.org/10.1186/s13568-020-01007-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7165217PMC

Effect of Aso limonite on anaerobic digestion of waste sewage sludge.

AMB Express 2020 Apr 16;10(1):74. Epub 2020 Apr 16.

Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku, Kitakyushu, 808-0196, Japan.

The effect of Aso volcanic limonite was explored in anaerobic digestion using waste sewage sludge (WSS). In this study, methane and hydrogen sulfide were remarkably inhibited when Aso limonite was mixed with WSS as well as a significant reduction of ammonia. Although pH was lowered after adding Aso limonite, methane was still inhibited in neutralized pH condition at 7. Read More

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http://dx.doi.org/10.1186/s13568-020-01010-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162999PMC

Production, characterization and bioinformatics analysis of L-asparaginase from a new Stenotrophomonas maltophilia EMCC2297 soil isolate.

AMB Express 2020 Apr 15;10(1):71. Epub 2020 Apr 15.

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, African Union Organization St., Abbassia, Cairo 11566, Egypt.

An exhaustive screening program was applied for scoring a promising L-asparaginase producing-isolate. The recovered isolate was identified biochemically and molecularly and its L-asparaginase productivity was optimized experimentally and by Response Surface Methodology. The produced enzyme was characterized experimentally for its catalytic properties and by bioinformatics analysis for its immunogenicity. Read More

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http://dx.doi.org/10.1186/s13568-020-01005-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7158977PMC

Ameliorating process parameters for zeaxanthin yield in Arthrobacter gandavensis MTCC 25325.

AMB Express 2020 Apr 15;10(1):69. Epub 2020 Apr 15.

Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201 002, India.

The present study aims to escalate the production of prophylactic agent zeaxanthin using a screened potential bacterial isolate. For this purpose, a freshwater bacterium capable of producing zeaxanthin was isolated from Bor Talav, Bhavnagar. The 16S rRNA sequence confirmed the isolate as Arthrobacter gandavensis. Read More

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http://dx.doi.org/10.1186/s13568-020-01008-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7158978PMC

Exploring the key microbial changes in the rhizosphere that affect the occurrence of tobacco root-knot nematodes.

AMB Express 2020 Apr 15;10(1):72. Epub 2020 Apr 15.

College of Plant Protection, Southwest University, Chongqing, 400715, China.

Root-knot nematode (RKN) disease is a soil-borne disease. However, most studies on RKN have focused on the screening of agents and the cultivation of resistant varieties, and reports on the interaction of RKNs with soil microorganisms are few. In this study, we performed Illumina high-throughput sequencing to analyze diseased and healthy soil and the microbial-community changes in rhizosphere soil after microbial treatment (Pseudomonas flurescens, Bacillus subtilis, Paecolomyces lilacinus). Read More

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http://dx.doi.org/10.1186/s13568-020-01006-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7158972PMC

Heterologous expression of genes for bioconversion of xylose to xylonic acid in Corynebacterium glutamicum and optimization of the bioprocess.

AMB Express 2020 Apr 15;10(1):68. Epub 2020 Apr 15.

Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Thiruvananthapuram, 695019, Kerala, India.

In bacterial system, direct conversion of xylose to xylonic acid is mediated through NAD-dependent xylose dehydrogenase (xylB) and xylonolactonase (xylC) genes. Heterologous expression of these genes from Caulobacter crescentus into recombinant Corynebacterium glutamicum ATCC 13032 and C. glutamicum ATCC 31831 (with an innate pentose transporter, araE) resulted in an efficient bioconversion process to produce xylonic acid from xylose. Read More

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http://dx.doi.org/10.1186/s13568-020-01003-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7158973PMC

Construction of engineered yeast producing ammonia from glutamine and soybean residues (okara).

AMB Express 2020 Apr 15;10(1):70. Epub 2020 Apr 15.

Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo‑ku, Kyoto, 606‑8502, Japan.

Ammonia is an essential substance for agriculture and the chemical industry. The intracellular production of ammonia in yeast (Saccharomyces cerevisiae) by metabolic engineering is difficult because yeast strongly assimilates ammonia, and the knockout of genes enabling this assimilation is lethal. Therefore, we attempted to produce ammonia outside the yeast cells by displaying a glutaminase (YbaS) from Escherichia coli on the yeast cell surface. Read More

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http://dx.doi.org/10.1186/s13568-020-01011-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7158961PMC

Systematic approach for assessing whether undeletable chromosomal regions in Saccharomyces cerevisiae are required for cell viability.

AMB Express 2020 Apr 15;10(1):73. Epub 2020 Apr 15.

Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Ikeda 4-22-1, Nishi-ku, Kumamoto, 860-0082, Japan.

Previously, we identified 49 undeletable chromosomal regions harboring only non-essential genes in the genome of Saccharomyces cerevisiae. We proposed that there might be unknown synthetic lethal combinations of genes present in such undeletable regions of the genome. In this study, we chose four of the smallest undeletable chromosomal regions among the 49 and performed extensive further analyses to narrow down the gene-pairs responsible for lethality by replacing sub-regions in various combinations with a DNA module comprising the CgLEU2 marker. Read More

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http://dx.doi.org/10.1186/s13568-020-01001-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7158983PMC

Directed evolution of a transcription factor PbrR to improve lead selectivity and reduce zinc interference through dual selection.

AMB Express 2020 Apr 10;10(1):67. Epub 2020 Apr 10.

Department of Chemical Engineering, University of New Hampshire, Durham, NH, 03824, USA.

Directed evolution has been proven as a powerful tool for developing proteins and strains with novel or enhanced features. In this study, a dual selection system was designed to tune the binding specificity of a transcription factor to a particular ligand with the ampicillin resistance gene amp (ON selection) as the positive selection marker and the levansucrase gene sacB (OFF selection) as the negative selection marker. It was applied to the lead responsive transcription factor PbrR in a whole-cell lead biosensor previously constructed in our lab (Jia et al. Read More

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http://dx.doi.org/10.1186/s13568-020-01004-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148400PMC

Silver ion bioreduction in nanoparticles using Artemisia annua L. extract: characterization and application as antibacterial agents.

AMB Express 2020 Apr 7;10(1):66. Epub 2020 Apr 7.

Department of Biochemistry, Microbiology and Biotechnology, Biology Faculty, Yerevan State University, 1 A. Manoukian Str., 0025, Yerevan, Armenia.

The biological synthesis of metal nanoparticles using plant extracts with defined size and morphology is a simple, nontoxic and environmentally friendly method. The present study focused on the synthesis of silver nanoparticles (Ag NPs) by Artemisia annua L. extract as reducing and stabilising agent. Read More

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http://dx.doi.org/10.1186/s13568-020-01002-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138892PMC

Increased resistance of a methicillin-resistant Staphylococcus aureus Δagr mutant with modified control in fatty acid metabolism.

AMB Express 2020 Apr 7;10(1):64. Epub 2020 Apr 7.

Department of Biological Engineering, College of Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea.

Methicillin-resistant Staphylococcus aureus (MRSA) strains are distinct from general Staphylococcus strains with respect to the composition of the membrane, ability to form a thicker biofilm, and, importantly, ability to modify the target of antibiotics to evade their activity. The agr gene is an accessory global regulator of gram-positive bacteria that governs virulence or resistant mechanisms and therefore an important target for the control of resistant strains. However, the mechanism by which agr impacts resistance to β-lactam antibiotics remains unclear. Read More

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http://dx.doi.org/10.1186/s13568-020-01000-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138893PMC

A simple and rapid protein purification method based on cell-surface display of SUMO-fused recombinant protein and Ulp1 protease.

AMB Express 2020 Apr 7;10(1):65. Epub 2020 Apr 7.

Zhejiang Provincial Key Laboratory for Technology and Application of Model Organisms, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, 325035, Zhejiang, China.

The development of novel methods for highly efficient protein purification remains a research focus in the biotechnology field because conventional purification approaches, including affinity purification, gel filtration, and ion-exchange chromatography, require complex manipulation steps and are costly. Here, we describe a simple and rapid protein purification strategy in which the SUMO tag and Ulp1 protease are surface-displayed separately on Escherichia coli cells. After protein induction, the cells are harvested, resuspended in cleavage buffer, and incubated together for cleavage. Read More

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http://dx.doi.org/10.1186/s13568-020-00999-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138890PMC

Long-chain n-alkane biodegradation coupling to methane production in an enriched culture from production water of a high-temperature oil reservoir.

AMB Express 2020 Apr 7;10(1):63. Epub 2020 Apr 7.

State Key Laboratory of Bioreactor Engineering and School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People's Republic of China.

Paraffinic n-alkanes (C22-C30), crucial portions of residual oil, are generally considered to be difficult to be biodegraded owing to their general solidity at ambient temperatures and low water solubility, rendering relatively little known about metabolic processes in different methanogenic hydrocarbon-contaminated environments. Here, we established a methanogenic C22-C30 n-alkane-degrading enrichment culture derived from a high-temperature oil reservoir production water. During two-year incubation (736 days), unexpectedly significant methane production was observed. Read More

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http://dx.doi.org/10.1186/s13568-020-00998-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138878PMC

Transgenic plant generated by RNAi-mediated knocking down of soybean Vma12 and soybean mosaic virus resistance evaluation.

AMB Express 2020 Apr 6;10(1):62. Epub 2020 Apr 6.

National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.

Soybean mosaic virus (SMV) is one of the most destructive viral diseases in soybean and causes severe reduction of soybean yield and destroys the seed quality. However, the production of SMV resistant plants by transgenic is the most effective and economical means. Based on our previous yeast two-hybrid assay, the GmVma12 was selected as a strong candidate gene for further function characterization. Read More

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http://dx.doi.org/10.1186/s13568-020-00997-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7136382PMC

Microbial nitrogen fixation and methane oxidation are strongly enhanced by light in Sphagnum mosses.

AMB Express 2020 Mar 31;10(1):61. Epub 2020 Mar 31.

Department of Microbiology, Radboud University, Heijendaalseweg 135, 6525 AJ, Nijmegen, The Netherlands.

Peatlands have acted as C-sinks for millennia, storing large amounts of carbon, of which a significant amount is yearly released as methane (CH). Sphagnum mosses are a key genus in many peat ecosystems and these mosses live in close association with methane-oxidizing and nitrogen-fixing microorganisms. To disentangle mechanisms which may control Sphagnum-associated methane-oxidation and nitrogen-fixation, we applied four treatments to Sphagnum mosses from a pristine peatland in Finland: nitrogen fertilization, phosphorus fertilization, CH addition and light. Read More

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http://dx.doi.org/10.1186/s13568-020-00994-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7109220PMC

Combined ANN/EVOP Factorial Design Approach for Media Screening for Cost-effective Production of Alkaline Proteases from Rhizopus oryzae (SN5)/NCIM-1447 under SSF.

AMB Express 2020 Mar 27;10(1):60. Epub 2020 Mar 27.

Department of Biotechnology, Motilal Nehru National Institute of Technology Allahabad, Prayagraj, UP, 211004, India.

In order to achieve high yield of fungal protease in a very cost effective way and to meet its increased market demand, current study deals with the screening of various agro-wastes as carbon source for the production of protease from Rhizopus oryzae (SN5)/NCIM-1447 under solid state fermentation. Substrates and culture parameters such as wheat bran, soybean meal, black-gram husk, rice husk, mixture of wheat bran, soybean meal, nitrogen sources, pH, temperature and incubation time were first optimized with one factor at time strategy and then EVOP factorial and yield of alkaline protease was achieved 412.8 U/gds at 28 °C and pH = 6 after 72 h of fermentation taking wheat bran and soybean as a substrate in 4:1 ratio. Read More

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http://dx.doi.org/10.1186/s13568-020-00996-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101461PMC

Bacterial delivery of the anti-tumor azurin-like protein Laz to glioblastoma cells.

AMB Express 2020 Mar 27;10(1):59. Epub 2020 Mar 27.

Department of Microbiology and Immunology, The German University in Cairo (GUC), Main Entrance Fifth Settlement, Cairo, Egypt.

Salmonella typhimurium VNP-20009 (VNP) is a non-pathogenic attenuated strain, which, as a facultative anaerobe, preferentially accumulates in hypoxic regions of solid tumors. Here, VNP was utilized as a delivery vehicle of the anti-tumor protein Lipidated azurin, Laz, which is produced by the meningitis-causing bacterium Neisseria meningitides. In brain cancer cells, Laz has been demonstrated to induce apoptosis through an interaction with the tumor suppressor protein p53. Read More

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http://dx.doi.org/10.1186/s13568-020-00995-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7099546PMC

Kaempferol ameliorates Cisplatin induced nephrotoxicity by modulating oxidative stress, inflammation and apoptosis via ERK and NF-κB pathways.

AMB Express 2020 Mar 26;10(1):58. Epub 2020 Mar 26.

Department of Nephrology, Xijing hospital, The fourth military medical university, Xi'an, 710032, Shaanxi, China.

Anticancer drug like Cisplatin are associated with serious problem like nephrotoxicity. The effect of Kaempferol is a plant-derived flavonoid compound. The present work evaluated the effect of Kaempferol in mouse model of Cisplatin mediated nephrotoxicity also the involved mechanism. Read More

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http://dx.doi.org/10.1186/s13568-020-00993-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7098399PMC

Anserine and glucosamine supplementation attenuates the levels of inflammatory markers in rats with rheumatoid arthritis.

AMB Express 2020 Mar 20;10(1):57. Epub 2020 Mar 20.

Department of Orthopaedics, The Second Affiliated Hospital of Nanchang University, No 1 Minde Road, Donghu District, Nanchang City, 330000, JiangXi Province, China.

Rheumatoid arthritis (RA) is an autoimmune disorder that affects the joint synovium. Anserine is a functional dipeptide containing methylhistidine and β-alanine, and is present in the brain and skeletal muscle of birds and mammals. Glucosamine is an amino sugar used in the synthesis of glycosylated proteins and lipids. Read More

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http://dx.doi.org/10.1186/s13568-020-00987-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7083984PMC

Therapeutic efficacy of catalpol against apoptosis in cardiomyocytes derived from human induced pluripotent stem cells.

AMB Express 2020 Mar 20;10(1):56. Epub 2020 Mar 20.

Department of Cardiology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, 1665 Kongjiang Road, Shanghai, 200092, China.

Cardiac arrhythmia is an irregular heart rhythm that can lead to serious heart conditions and various organ disorders, and may cause sudden cardiac death. Catalpol belongs to the iridoid glycoside family and is highly abundant in Rehmannia glutinosa Libosch. The study included five groups such as group I (normal control), group II (treatment control), group III (low-dose treatment), group IV (medium-dose treatment) and group V (high-dose treatment). Read More

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http://dx.doi.org/10.1186/s13568-020-00986-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7083986PMC

600-ns pulsed electric fields affect inactivation and antibiotic susceptibilities of Escherichia coli and Lactobacillus acidophilus.

AMB Express 2020 Mar 18;10(1):55. Epub 2020 Mar 18.

Radio Frequency Bioeffects Branch, Bioeffects Division, Airman Systems Directorate, 711th Human Performance Wing, Air Force Research Laboratory, JBSA, Fort Sam Houston, San Antonio, TX, USA.

Cell suspensions of Escherichia coli and Lactobacillus acidophilus were exposed to 600-ns pulsed electric fields (nsPEFs) at varying amplitudes (Low-13.5, Mid-18.5 or High-23. Read More

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http://dx.doi.org/10.1186/s13568-020-00991-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7080936PMC