17,468 results match your criteria Am. J. Clin. Pathol.[Journal]


H-index and Academic Medicine.

Am J Clin Pathol 2019 Apr 17. Epub 2019 Apr 17.

Biomedical Branch Library University of British Columbia Vancouver, Canada.

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http://dx.doi.org/10.1093/ajcp/aqz037DOI Listing

Outcome of Electronic Order Alert Intervention Relative to Toxigenic Clostridium difficile PCR Analysis and Hospital-Onset C difficile Infection in a Multihospital Health Care System.

Am J Clin Pathol 2019 Apr 16. Epub 2019 Apr 16.

Wisconsin Clinical Laboratory Network Technical Advisory Group, Madison.

Objectives: Concern exists regarding overdiagnosis of Clostridium difficile infection (CDI) via molecular modalities. We determined effects of a preanalytic order intervention on laboratory and CDI prevention measures in a multihospital system.

Methods: Intervals before and following implementation of a CDI electronic order alert (relative to appropriate testing scenario) were assessed for C difficile test volume and positivity rate, hospital-onset CDI frequency, and hospital-onset C difficile standardized infection ratio (SIR). Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqz022DOI Listing
April 2019
1 Read

Isolated Bone Marrow Non-Langerhans Cell Histiocytosis Preceding RUNX1-Mutated Acute Myeloid Leukemia.

Am J Clin Pathol 2019 Apr 16. Epub 2019 Apr 16.

Division of Hematology and HSCT, Department of Oncology, King Abdul Aziz Medical City, Riyadh, KSA.

Objectives: The prevalence of concomitant myeloid neoplasms was recently reported to be unexpectedly high among adults without non-Langerhans cell histiocytosis (non-LCH); however, the coexistence of non-LCH with RUNX1 genetic aberration has not been reported previously.

Methods: Herein, we report a 23-year-old woman with severe pancytopenia diagnosed with non-LCH following presentation with pancytopenia and marrow examination showing histiocytosis positive for CD45, CD68, CD136, and lysozyme but negative for CD1a, langerin, and S100.

Results: Whole-exome sequencing showed RUNX1 mutation and NF1 mutation. Read More

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http://dx.doi.org/10.1093/ajcp/aqz018DOI Listing
April 2019
1 Read

Quantitative Cytologic Descriptors to Differentiate CLL, Sézary, Granular, and Villous Lymphocytes Through Image Analysis.

Am J Clin Pathol 2019 Apr 16. Epub 2019 Apr 16.

Department of Mathematics, Barcelona Est Engineering School, Technical University of Catalonia, Barcelona, Spain.

Objectives: We aimed to find descriptors to identify chronic lymphocytic leukemia (CLL), Sézary, granular, and villous lymphocytes among normal and abnormal lymphocytes in peripheral blood.

Methods: Image analysis was applied to 768 images from 15 different types of lymphoid cells and monocytes to determine four discriminant descriptors. For each descriptor, numerical scales were obtained using 627 images from 79 patients. Read More

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http://dx.doi.org/10.1093/ajcp/aqz025DOI Listing

Laboratory Blood-Based Testing for Lyme Disease at a National Reference Laboratory.

Am J Clin Pathol 2019 Apr 15. Epub 2019 Apr 15.

Quest Diagnostics, Secaucus, NJ.

Objectives: We evaluated trends in Lyme disease (LD) testing at a national reference laboratory.

Methods: LD screening enzyme immunoassay and Western blot testing data performed at Quest Diagnostics during 2010 to 2016 were analyzed nationally and at the state level.

Results: Overall, 593,800 (11. Read More

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http://dx.doi.org/10.1093/ajcp/aqz030DOI Listing
April 2019
5 Reads

A Retrospective Analysis of Corrected Laboratory Results in a Large Academic Medical Center.

Authors:
Derick A Kalt

Am J Clin Pathol 2019 Apr 15. Epub 2019 Apr 15.

Master of Science Clinical Laboratory Management Program, Rush University, Chicago, IL.

Objectives: To evaluate the total number of corrected laboratory results that occurred as a consequence of errors in the preanalytical, analytical, and postanalytical phases of testing and to identify areas for improvement in patient care.

Methods: Revised laboratory results were retroactively reviewed for a 6-month period. The total number of revised results were categorized by department and the phase in which the resulting error occurred. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqz036DOI Listing
April 2019
1 Read

Evaluation of AdvanSure AlloScreen Max Panel With 92 Different Allergens for Detecting Allergen-Specific IgE.

Am J Clin Pathol 2019 Apr 15. Epub 2019 Apr 15.

Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul.

Objectives: The objective of this study was to examine the performance of AdvanSure AlloScreen Max with 92 different allergens compared to Polycheck Allergy and ImmunoCAP. The relationship of serum IgE concentration with the number and the highest class/level of positive allergen-specific IgEs was also examined.

Methods: A total of 406 serum samples were included in this study. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqz023DOI Listing
April 2019
1 Read

The PD-1/PD-L1 Axis in HER2+ Ductal Carcinoma In Situ (DCIS) of the Breast.

Am J Clin Pathol 2019 Apr 15. Epub 2019 Apr 15.

Breast Pathology Section, Department of Pathology, Northwestern University Feinberg School of Medicine, Robert H. Lurie Comprehensive Cancer Center, Chicago, IL.

Objectives: The aims were to evaluate the programmed death 1 (PD-1)/programmed death ligand 1 (PD-L1) axis in ductal carcinoma in situ (DCIS) of the breast.

Methods: We reviewed 85 pure DCIS cases treated with surgical excision at our institution, including 51 luminal A (estrogen receptor [ER] positive/human epidermal growth factor 2 [HER2] negative), 15 luminal B (ER+/HER2+), 13 HER2 (ER-/HER2+), and six basal-like (ER-/HER2-/CK5/6+). The extent and intensity of PD-1 and PD-L1 immunohistochemical staining in the tumor-infiltrating lymphocytes (TILs) and in the tumor cells were recorded. Read More

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http://dx.doi.org/10.1093/ajcp/aqz020DOI Listing

Impact of the 2018 ASCO/CAP HER2 Guideline Focused Update.

Am J Clin Pathol 2019 Apr 8. Epub 2019 Apr 8.

Department of Pathology, The Ohio State University Wexner Medical Center, Columbus.

Objectives: The 2018 American Society of Clinical Oncology/College of American Pathologists (ASCO/CAP) human epidermal growth factor receptor 2 (HER2) guideline focused update revises the HER2 scoring criteria. We evaluated the impact on HER2 rates in breast carcinoma diagnosed at our center.

Methods: In a retrospective series of breast core biopsies with invasive carcinoma diagnosed between 2014 and 2017 (n = 1,350), HER2 status was classified according to 2013 and 2018 ASCO/CAP guidelines and changes in HER2 status identified. Read More

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http://dx.doi.org/10.1093/ajcp/aqz012DOI Listing
April 2019
1 Read
2.514 Impact Factor

Stratification of Follicular Lymphoma.

Authors:
Steven H Kroft

Am J Clin Pathol 2019 Mar 28. Epub 2019 Mar 28.

Medical College of Wisconsin, Milwaukee.

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http://dx.doi.org/10.1093/ajcp/aqz008DOI Listing
March 2019
1 Read

p75 Nerve Growth Factor Receptor as a Specific Nerve Marker in the Diagnosis of Perineural Invasion of Squamous Cell Carcinoma.

Am J Clin Pathol 2019 Mar 20. Epub 2019 Mar 20.

Department of Stomatology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Objectives: Perineural invasion (PNI) is an important factor in tumor prognosis. We evaluated p75 nerve growth factor receptor (p75NGFR) as a neuromarker for perineural invasion of squamous cell carcinoma.

Methods: A comparison of H&E, and S100 and p75NGFR immunohistochemical staining methods, using sequential sections from 29 samples, for identification nerve bundles and PNI diagnosis, was carried out. Read More

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http://dx.doi.org/10.1093/ajcp/aqz011DOI Listing
March 2019
3 Reads

Effects of Improved DNA Integrity by Punch From Tissue Blocks as Compared to Pinpoint Extraction From Unstained Slides on Next-Generation Sequencing Quality Metrics.

Am J Clin Pathol 2019 Mar 20. Epub 2019 Mar 20.

University of Alabama at Birmingham.

Objectives: To compare the effects of two methods of formalin-fixed paraffin-embedded (FFPE) tissue harvesting on DNA quality and next-generation sequencing (NGS) quality metrics.

Methods: DNA integrity number (DIN) and NGS quality metrics resulting from DNA extraction and sequencing of 199 sequential samples harvested via the Pinpoint Slide DNA Isolation System and the punch method were compared.

Results: DNA extracted from FFPE tissue punches had higher DIN than that extracted from Pinpoint samples (mean ± SD, 6. Read More

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http://dx.doi.org/10.1093/ajcp/aqz014DOI Listing
March 2019
1 Read

Frozen-Section Checklist Implementation Improves Quality and Patient Safety.

Am J Clin Pathol 2019 Mar 20. Epub 2019 Mar 20.

Beth Israel Deaconess Medical Center, Pathology, Boston, MA.

Objectives: An intraoperative consultation (IOC) checklist was developed and implemented aimed at standardizing slide labeling and monitoring metrics central to quality and safety in surgical pathology.

Design: Data were collected for all IOC cases over a 9-month period. Slide labeling defect rates and IOC turnaround time (TAT) were recorded and compared for the pre- and postimplementation periods. Read More

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http://dx.doi.org/10.1093/ajcp/aqz009DOI Listing

Pathologic Features and Clinical Implications of Breast Cancer With HER2 Intratumoral Genetic Heterogeneity.

Am J Clin Pathol 2019 Mar 20. Epub 2019 Mar 20.

Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH.

Objectives: To review breast cancer with human epidermal growth factor receptor 2 (HER2) genetic heterogeneity (GH), with a focus on clinician interpretation of the results and subsequent management.

Methods: We retrospectively searched all consecutive invasive breast cancers that underwent HER2 fluorescent in situ hybridization testing and identified cases with HER2 GH.

Results: Eleven (0. Read More

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http://dx.doi.org/10.1093/ajcp/aqz010DOI Listing
March 2019
4 Reads

Widespread Implementation of the Hoffmann Method: A Second Opinion.

Am J Clin Pathol 2019 Mar 20. Epub 2019 Mar 20.

Biostatistics and Medical Informatics University of Wisconsin Madison.

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http://dx.doi.org/10.1093/ajcp/aqz015DOI Listing

Gross Dissection Time Values of Pathologists' Assistants Using Standardized Metrics.

Am J Clin Pathol 2019 Mar 18. Epub 2019 Mar 18.

Department of Pathology and Laboratory Medicine, Donald and Barbara Zucker School of Medicine of Medicine at Hofstra/Northwell, Hempstead, NY.

Objectives: A validated and objective method to quantify the gross dissection time of pathologists' assistants (PAs) does not exist. We propose a method to calculate standardized work units (dissection time values [DTVs]) to monitor PA productivity.

Methods: The Current Procedural Terminology system specifies six levels of specimen complexity encompassing 176 unique specimen types. Read More

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http://dx.doi.org/10.1093/ajcp/aqz007DOI Listing

Use of a Blast Dominance-Hematogone Index for the Flow Cytometric Evaluation of Myelodysplastic Syndrome (MDS).

Am J Clin Pathol 2019 Mar 11. Epub 2019 Mar 11.

Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA.

Objectives: We tested whether combined flow cytometric assessment of loss of blast heterogeneity and decreased hematogones is a diagnostically useful approach for evaluation of myelodysplastic syndrome (MDS).

Methods: Bone marrow samples from patients with known MDS were analyzed by 10-color flow cytometric immunophenotyping and compared with normal bone marrow samples.

Results: There was loss of blast heterogeneity in patients with MDS compared with normal bone marrow samples, based on the relative size of the dominant blast population (83. Read More

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http://dx.doi.org/10.1093/ajcp/aqz004DOI Listing
March 2019
1 Read

Ki-67 and p16 Immunostaining Differentiates Pagetoid Bowen Disease From "Microclonal" Seborrheic Keratosis.

Am J Clin Pathol 2019 Mar 10. Epub 2019 Mar 10.

Department of Dermatology and University of California, San Francisco.

Objectives: We observed keratoses with "clonal" nests present as numerous tiny collections, in which cells in "pagetoid" array are found, a configuration we termed microclonal seborrheic keratosis (MSK). To better distinguish MSK from pagetoid Bowen disease (PBD), we investigated use of immunohistochemical staining.

Methods: Biopsy specimens of 26 MSKs, 17 PBDs, and 11 borderline cases were reviewed for histopathology and stained with p53, Ki-67, and p16. Read More

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http://dx.doi.org/10.1093/ajcp/aqz001DOI Listing
March 2019
1 Read

Application of the Milan System for Reporting Salivary Gland Cytopathology.

Am J Clin Pathol 2019 Mar 10. Epub 2019 Mar 10.

Department of Pathology and Laboratory Medicine, Taipei Veterans General Hospital, Taipei, Taiwan.

Objectives: Multi-institutional studies are required for the validation of the Milan System for Reporting Salivary Gland Cytopathology (MSRSGC).

Methods: A total of 1,560 fine-needle aspirations of the salivary glands were retrieved from two institutions for a 12-year period. The diagnoses were reclassified based on the MSRSGC. Read More

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http://dx.doi.org/10.1093/ajcp/aqz006DOI Listing
March 2019
2.514 Impact Factor

Xanthomatous Thymoma.

Am J Clin Pathol 2019 Feb 28. Epub 2019 Feb 28.

Department of Pathology, University of Texas MD Anderson Cancer Center, Houston.

Objectives: Ten cases of thymomas with an extensive xanthomatous component are presented.

Methods: Cases were identified during a review of more than 500 cases of thymomas. Histologic material from surgical resections was evaluated. Read More

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http://dx.doi.org/10.1093/ajcp/aqz005DOI Listing
February 2019
2 Reads

The Utility of Phosphohistone H3 (PHH3) in Follicular Lymphoma Grading.

Am J Clin Pathol 2019 Feb 21. Epub 2019 Feb 21.

Department of Pathology, Brooke Army Medical Center, San Antonio, TX.

Objectives: To determine the utility of phosphohistone H3 (PHH3) mitotic count (MC) in grading follicular lymphoma (FL).

Methods: FL cases were identified (2005-2017). Three hematopathologists recorded their average Ki-67 proliferation index, MC/high-power field (hpf) using PHH3 and H&E stains, and number of centroblasts/hpf. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqz003DOI Listing
February 2019
1 Read

PD-L1 Expression Reveals Significant Association With Squamous Differentiation in Upper Tract Urothelial Carcinoma.

Am J Clin Pathol 2019 Feb 18. Epub 2019 Feb 18.

Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia.

Objectives: Limited literature is available on the tumor microenvironment (TM) of upper tract urothelial carcinoma (UTUC). This study comprehensively reviews programmed death 1 receptor (PD-1)-positive and CD8+ tumor-infiltrating lymphocytes (TILs) and programmed death ligand 1 (PD-L1) expression on tumor epithelium (TE).

Methods: Seventy-two nephroureterectomy specimens were analyzed for PD-L1, PD-1, and CD8. Read More

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http://dx.doi.org/10.1093/ajcp/aqz002DOI Listing
February 2019

Isolated Flat Epithelial Atypia on Core Biopsy Specimens Is Associated With a Low Risk of Upgrade at Excision.

Am J Clin Pathol 2019 Apr;151(5):511-515

University of Pittsburgh Medical Center, Pittsburgh, PA.

Objectives: We present a retrospective review of 111 breast core biopsy specimens with flat epithelial atypia (FEA) and corresponding excision to better understand rates of upgrade following this diagnosis.

Methods: In total, 252 breast core biopsy specimens were identified. Cases were excluded if biopsy slides or excision results were unavailable, for ipsilateral carcinoma or other findings warranting excision, or biopsy performed for indications other than mammographically detected calcifications. Read More

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http://dx.doi.org/10.1093/ajcp/aqy175DOI Listing

Correction.

Authors:

Am J Clin Pathol 2019 Feb;151(3):349

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https://academic.oup.com/ajcp/article/151/3/349/5306290
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http://dx.doi.org/10.1093/ajcp/aqy177DOI Listing
February 2019
4 Reads

Detection of Nicotine and Nicotine Metabolites in Units of Banked Blood.

Am J Clin Pathol 2019 Apr;151(5):516-521

Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN.

Objectives: To determine the concentrations of nicotine and nicotine metabolites in RBC units as a means to estimate the point prevalence of exposure within the healthy donor pool.

Methods: Segments from 105 RBC units were tested for the presence of nicotine, cotinine, or trans-3'-hydroxycotinine by liquid chromatography-tandem mass spectrometry.

Results: Of the 20 (19%) units that contained detectable concentrations of nicotine, cotinine, or trans-3'-hydroxycotinine, 19 (18. Read More

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http://dx.doi.org/10.1093/ajcp/aqy176DOI Listing

Susceptibility of Cardiac Troponin Assays to Biotin Interference.

Am J Clin Pathol 2019 Apr;151(5):486-493

Department of Pathology, Clements University Hospital, University of Texas Southwestern Medical Center, Dallas.

Objectives: To investigate biotin interference on three cardiac troponin (cTn) assays and demonstrate a method to overcome biotin interference.

Methods: cTn levels were measured in (1) plasma from healthy volunteers on 10-mg daily biotin supplementation mixed with a plasma with known elevated troponin, (2) plasmas with known elevated cTn after mixing in reagent biotin to simulate supplementation, and (3) biotin-spiked plasma specimens pretreated with streptavidin-agarose beads.

Results: Daily biotin ingestion (10 mg) and studies simulating daily biotin use resulted in significant interference in the Gen5 cardiac troponin T (cTnT) assay; the contemporary Gen 4 cTnT and high-sensitivity cardiac troponin I (hs-cTnI) assays were unaffected. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy172DOI Listing
April 2019
16 Reads

Utility of a Simple and Robust Flow Cytometry Assay for Rapid Clonality Testing in Mature Peripheral T-Cell Lymphomas.

Am J Clin Pathol 2019 Apr;151(5):494-503

Department of Pathology, Brigham and Women's Hospital, Boston, MA.

Objectives: Flow cytometry immunophenotyping is limited by poor resolution of T-cell clones. A newly described antibody was recently used to distinguish normal peripheral blood T cells from malignant T-cell clones. Here, we evaluate this antibody as a new diagnostic tool for detecting T-cell clonality in mature peripheral T-cell lymphomas. Read More

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http://dx.doi.org/10.1093/ajcp/aqy173DOI Listing

Immunohistochemical Nuclear Expression of β-Catenin as a Surrogate of CTNNB1 Exon 3 Mutation in Endometrial Cancer.

Am J Clin Pathol 2019 Apr;151(5):529-538

Gynecology and Obstetrics Unit, Department of Neuroscience, Reproductive Sciences and Dentistry, School of Medicine.

Objectives: CTNNB1 exon 3 mutations have shown independent prognostic value in endometrial cancer. We aimed to assess whether nuclear β-catenin expression is an accurate surrogate, as immunohistochemistry is cheaper, faster, and more widely applicable than sequencing.

Methods: A systematic review was performed by searching electronic databases for all studies assessing the association between β-catenin immunohistochemical expression and CTNNB1 mutations. Read More

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http://dx.doi.org/10.1093/ajcp/aqy178DOI Listing
April 2019
2.514 Impact Factor

Time-Series Analysis of Laboratory Values in the Context of Long-Term Hospitalized Patient Mortality.

Am J Clin Pathol 2019 Apr;151(5):452-460

Department of Pathology, State University of New York Downstate Medical Center, Brooklyn.

Objectives: Long-term hospitalized patients have a higher risk of adverse outcomes and mortality rate. These patients often rapidly deteriorate, leading to death. We aim to evaluate end-of-life laboratory values time trends among deceased long-term inpatients. Read More

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http://dx.doi.org/10.1093/ajcp/aqy163DOI Listing
April 2019
1 Read

The FDA-Approved Breast Cancer HER2 Evaluation Kit (HercepTest; Dako) May Miss Some HER2-Positive Breast Cancers.

Am J Clin Pathol 2019 Apr;151(5):504-510

Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA.

Objectives: Accurate evaluation of human epidermal growth factor receptor 2 (HER2) in breast cancer is critical.

Methods: HER2 fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) tests were performed on 52 cases using a US Food and Drug Administration (FDA)-approved kit (HercepTest, FDA kit) and a laboratory-developed test (LDT) with the HercepTest antibody and a Leica Bond automated stainer.

Results: By FISH, 22 were HER2 positive, 29 were negative, and one was equivocal. Read More

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http://dx.doi.org/10.1093/ajcp/aqy171DOI Listing
April 2019
1 Read

Radiofrequency Interference in the Clinical Laboratory.

Am J Clin Pathol 2019 Apr;151(5):522-528

Department of Pathology and Laboratory Medicine, Nationwide Children's Hospital, Columbus, OH.

Objectives: Radiofrequency interference (RFI) is a known medical device safety issue, but there are no documented cases of interference resulting in erroneous laboratory results.

Methods: We investigated unexpected failure of a hematology analyzer resulting in erroneous WBC counts. Hardware failure was initially suspected, but temporal association with increased power output from a nearby antenna prompted investigation for RFI. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy174DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6445342PMC
April 2019
4 Reads

Diagnostic Utility of UroVysion Combined With Conventional Urinary Cytology for Urothelial Carcinoma of the Upper Urinary Tract.

Am J Clin Pathol 2019 Apr;151(5):469-478

Department of Surgical Pathology, Aichi Medical University Hospital, Nagakute, Japan.

Objectives: We prospectively evaluated the utility of UroVysion in urothelial carcinomas of the upper urinary tract (UCUUTs).

Methods: Ninety patients who received nephroureterectomy for UCUUT were enrolled. We performed urinary cytology and UroVysion before nephroureterectomy. Read More

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http://dx.doi.org/10.1093/ajcp/aqy170DOI Listing

HPV Testing With 16, 18, and 45 Genotyping Stratifies Cancer Risk for Women With Normal Cytology.

Am J Clin Pathol 2019 Mar;151(4):433-442

Becton, Dickinson and Company, BD Life Sciences-Diagnostic Systems, Sparks, MD.

Objectives: To determine the BD Onclarity human papillomavirus (HPV) assay performance and risk values for cervical intraepithelial neoplasia grade 2 (CIN2) or higher and cervical intraepithelial neoplasia grade 3 (CIN3) or higher during Papanicolaou/HPV cotesting in a negative for intraepithelial lesions or malignancies (NILM) population.

Methods: In total, 22,383 of the 33,858 enrolled women were 30 years or older with NILM cytology. HPV+ and a subset of HPV- patients (3,219/33,858 combined; 9. Read More

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http://dx.doi.org/10.1093/ajcp/aqy169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396747PMC
March 2019
4 Reads

Impact of Specimen Type and Specimen Number on HER2 Status in Gastroesophageal Junction and Gastric Adenocarcinoma.

Am J Clin Pathol 2019 Apr;151(5):461-468

Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY.

Objectives: Human epidermal growth factor receptor 2 (HER2) is expressed in some gastric and gastroesophageal junction adenocarcinomas. There were two goals: assess the impact of specimen type on HER2 status and evaluate HER2 concordance with multiple specimens.

Methods: All cases were evaluated by immunohistochemistry (IHC) for HER2 and interpreted using established criteria. Read More

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http://dx.doi.org/10.1093/ajcp/aqy166DOI Listing
April 2019
1 Read

Correction.

Authors:

Am J Clin Pathol 2019 Jan;151(2):240

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https://academic.oup.com/ajcp/article/151/2/240/5261363
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http://dx.doi.org/10.1093/ajcp/aqy159DOI Listing
January 2019
1 Read

Rete Testis Invasion Is Consistent With Pathologic Stage T1 in Germ Cell Tumors.

Am J Clin Pathol 2019 Apr;151(5):479-485

Medical College of Wisconsin, Milwaukee.

Objectives: Rete testis invasion by germ cell tumors is frequently concomitant with lymphovascular or spermatic cord invasion (LVI/SCI); independent implications for staging are uncertain.

Methods: In total, 171 seminomas and 178 nonseminomatous germ cell tumors (NSGCTs; 46 had 1%-60% seminoma component) came from five institutions. Metastatic status at presentation, as a proxy for severity, was available for all; relapse data were unavailable for 152. Read More

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http://fdslive.oup.com/www.oup.com/pdf/production_in_progres
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http://dx.doi.org/10.1093/ajcp/aqy168DOI Listing
April 2019
3 Reads

Application of Fluorescence In Situ Hybridization on Cerebrospinal Fluid Cytospins for the Detection of Residual Leukemic Cells in Patients With Childhood Acute Lymphoblastic Leukemia.

Am J Clin Pathol 2019 Mar;151(4):416-423

Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.

Objectives: Diagnosis of central nervous system involvement in acute lymphoblastic leukemia (ALL) requires morphologic expertise; therefore, we evaluated interphase fluorescence in situ hybridization (iFISH) of cerebrospinal fluid (CSF) cytospin preparations as a potential complementary test.

Methods: Twenty-three CSF cytospin specimens from 13 pediatric patients with ALL were included. iFISH probes detecting BCR-ABL1, ETV6-RUNX1, and KMT2A rearrangement and CDKN2A deletion, which were present at initial diagnosis, were used on follow-up CSF cytospin specimens and were compared with cytology. Read More

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http://dx.doi.org/10.1093/ajcp/aqy160DOI Listing
March 2019
2 Reads

Three Approaches to Creating an Essential Diagnostics List.

Am J Clin Pathol 2019 Apr;151(5):443-445

Department of Pathology and Laboratory Medicine, Johns Hopkins University, Baltimore, MD.

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
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http://dx.doi.org/10.1093/ajcp/aqy167DOI Listing
April 2019
14 Reads

Two Extraordinary Sellar Neuronal Tumors: Literature Review and Comparison of Clinicopathologic Features.

Am J Clin Pathol 2019 Feb;151(3):241-254

Department of Pathology, Duke University Medical Center, Durham, NC.

Objectives: The list of tumors involving the pituitary gland has been expanded to include a variety of neuronal and paraneuronal tumors in the 2017 World Health Organization tumor classification of endocrine organs. All the entities included in this category are distinctly rare, with limited case reports in the literature.

Methods: We illustrate two extraordinary sellar tumors with neuronal differentiation: a sellar paraganglioma and a sellar neurocytoma, with thorough literature review and comparison of the clinicopathologic features of these entities. Read More

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http://dx.doi.org/10.1093/ajcp/aqy155DOI Listing
February 2019
3 Reads

Establishing Commonsense-Based Statistical Quality Control Practices.

Am J Clin Pathol 2019 Mar;151(4):350-352

Service of Laboratory Medicine, Hospital General de Requena, Valencia, Spain.

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http://dx.doi.org/10.1093/ajcp/aqy162DOI Listing
March 2019
1 Read

Quality Improvement in Transfusion Practice of Orthotopic Liver Transplantation Reduces Blood Utilization, Length of Hospital Stay, and Cost.

Am J Clin Pathol 2019 Mar;151(4):395-402

Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN.

Objectives: Orthotopic liver transplantation (OLT) can require substantial usage of blood products. Higher rates of transfusion have been associated with increased length of hospital stay, higher rates of infection, graft failure, and mortality. This study was a retrospective analysis to assess the impact of quality improvement interventions in OLT. Read More

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http://dx.doi.org/10.1093/ajcp/aqy154DOI Listing
March 2019
1 Read

The Top 25 Laboratory Tests by Volume and Revenue in Five Different Countries.

Am J Clin Pathol 2019 Apr;151(5):446-451

Pathology and Laboratory Services, Denver Health Medical Center, Denver, CO.

Objectives: To compare the most common diagnostic/laboratory tests across five different referral hospitals by volume and revenue.

Methods: The authors obtained data on volumes and reimbursement rates for the most common 25 tests at the five hospitals with which they are affiliated and organized them to be as comparable as possible. Simple descriptive statistics were used to make cross-country comparisons. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
Publisher Site
http://dx.doi.org/10.1093/ajcp/aqy165DOI Listing
April 2019
22 Reads
2.514 Impact Factor

Assessment of Programmed Death-Ligand 1 (PD-L1) Immunohistochemical Expression on Cytology Specimens in Non-Small Cell Lung Carcinoma.

Am J Clin Pathol 2019 Mar;151(4):403-415

NYU Langone Medical Center, New York, NY.

Objectives: To evaluate whether non-small cell lung carcinoma (NSCLC) cytology specimens are reliable for programmed death-ligand 1 (PD-L1) immunohistochemical (IHC) testing.

Methods: Fifty-two cell blocks (CBs) with corresponding surgical pathology PD-L1 IHC testing were stained with a Dako PD-L1 pharmDX antibody (clone-22C3). Tumor cellularity was recorded as <100 or ≥100 cells. Read More

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http://dx.doi.org/10.1093/ajcp/aqy164DOI Listing
March 2019
1 Read

Diagnostic Utility of Isolated Tube C Positivity in T-Cell Receptor β Testing Using BIOMED-2 Primers.

Am J Clin Pathol 2019 Mar;151(4):386-394

Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA.

Objectives: T-cell receptor (TCR) gene rearrangement studies are widely used for assessing T-cell clonality. The frequency and significance of clonal peaks restricted to TCR β (TCRB) tube C are uncertain. We retrospectively reviewed 80 TCR studies performed on bone marrow/peripheral blood. Read More

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http://dx.doi.org/10.1093/ajcp/aqy157DOI Listing
March 2019
1 Read

Establishing Evidence-Based Statistical Quality Control Practices.

Am J Clin Pathol 2019 Mar;151(4):364-370

Westgard QC, Madison, WI.

Objectives: To establish an objective, scientific, evidence-based process for planning statistical quality control (SQC) procedures based on quality required for a test, precision and bias observed for a measurement procedure, probabilities of error detection and false rejection for different control rules and numbers of control measurements, and frequency of QC events (or run size) to minimize patient risk.

Methods: A Sigma-Metric Run Size Nomogram and Power Function Graphs have been used to guide the selection of control rules, numbers of control measurements, and frequency of QC events (or patient run size).

Results: A tabular summary is provided by a Sigma-Metric Run Size Matrix, with a graphical summary of Westgard Sigma Rules with Run Sizes. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
Publisher Site
http://dx.doi.org/10.1093/ajcp/aqy158DOI Listing
March 2019
4 Reads

Discordant Partial Thromboplastin Time (PTT) vs Anti-Xa Heparin Activity.

Am J Clin Pathol 2019 Mar;151(4):424-432

Department of Laboratory Medicine, University of Washington, Seattle.

Objectives: To determine the relationship between baseline variations in the partial thromboplastin time (PTT) and the discordance between the PTT and anti-Xa heparin activity (anti-Xa) during heparin therapy.

Methods: The baseline PTT on heparin was determined using automated heparin neutralization with protamine (prPTT). The prPTT was used to calculate a baseline-corrected PTT on heparin to reduce discordance with anti-Xa measurements. Read More

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http://dx.doi.org/10.1093/ajcp/aqy156DOI Listing
March 2019
1 Read

Widespread Incorrect Implementation of the Hoffmann Method, the Correct Approach, and Modern Alternatives.

Am J Clin Pathol 2019 Feb;151(3):328-336

Biostatistics and Medical Informatics, University of Wisconsin, Madison.

Objectives: The Hoffmann method is a procedure for reference interval estimation using routine clinical results. Many authors incorrectly prepare Hoffmann plots on a linear rather than normal probability scale. We explore the consequences. Read More

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http://dx.doi.org/10.1093/ajcp/aqy149DOI Listing
February 2019
1 Read

Comparison of Phenotypic Activated Protein C Resistance Testing With a Genetic Assay for Factor V Leiden.

Am J Clin Pathol 2019 Feb;151(3):302-305

Department of Laboratory Medicine, Division of Clinical Coagulation, University of Washington, Seattle.

Objectives: To compare the accuracy and reliability of phenotypic activated protein C resistance (aPC-R) assays with a genotypic assay for the factor V Leiden F5 p.R506Q (FVL) mutation.

Methods: Data were obtained from an electronic data warehouse for FVL testing performed at an academic institution with a large referral laboratory service. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
Publisher Site
http://dx.doi.org/10.1093/ajcp/aqy142DOI Listing
February 2019
3 Reads

Analytical and Clinical Performance Evaluation of the Elecsys HIV combi PT Assay on the cobas e 602 Analyzer for the Diagnosis of Human Immunodeficiency Virus.

Am J Clin Pathol 2019 Mar;151(4):377-385

Baylor Scott & White Health, Temple, TX.

Objectives: We evaluated the performance of the Elecsys HIV combi PT assay on the cobas e 602 analyzer for diagnosing human immunodeficiency virus (HIV; part of the US Food and Drug Administration [FDA] submission).

Methods: The HIV combi PT and reference (ARCHITECT HIV Ag/Ab Combo) assays were assessed at four independent clinical laboratories/one reference laboratory (United States; July 2014 to November 2015). Clinical performance was evaluated using four reagent lots. Read More

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https://academic.oup.com/ajcp/advance-article/doi/10.1093/aj
Publisher Site
http://dx.doi.org/10.1093/ajcp/aqy153DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396746PMC
March 2019
36 Reads