Search Our Scientific Publications & Authors

Vox Sang 2021 Jun 10. Epub 2021 Jun 10.

Department of Pediatrics, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Background And Objectives: The advent of intrauterine transfusion (IUT) has improved the survival of severe foetal anaemia. The aim of this study was to compare the perinatal outcomes of red blood cell (RBC)-alloimmunized pregnancies with anti-RhD in combination and anti-RhD alone in China.

Materials And Methods: A retrospective study was conducted involving RBC-alloimmunized pregnancies with anti-RhD in combination and anti-RhD alone admitted to The First Affiliated Hospital, Sun Yat-sen University, between January 2007 and December 2019. Read More

JBI Evid Synth 2021 May 28. Epub 2021 May 28.

School of Nursing, University of São Paulo (EEUSP), São Paulo, Brazil Clinics Hospital of Faculty of Medicine, University of São Paulo - HCFMUSP, São Paulo, Brazil The Brazilian Centre for Evidence-based Healthcare: A JBI Centre of Excellence, São Paulo, Brazil.

Objective: This review will aim to synthesize the best available evidence on the effectiveness of leukocyte reduction comparing post-storage and pre-storage filters regarding transfusion reactions, bacterial infection, length of stay, and mortality.

Introduction: Transfusion is a relevant therapy, but it is not risk-free. Leukocyte reduction can be performed either by apheresis or by pre- or post-storage filters in order to reduce the risk of transfusion reactions, transmission of some diseases, alloimmunization, and platelet refractoriness, in addition to the length of stay and the use of antibiotics. Read More

Clin Exp Immunol 2021 May 22. Epub 2021 May 22.

Department of Blood Transfusion, Guangzhou First People's Hospital, South China University of Technology, Guangzhou, Guangdong, China.

The clinical significance of the specific anti-John Milton Hagen (JMH) alloantibody in inherited JMH-negative patients remains unclear. During clinical blood transfusion, it is often classified as an anti-JMH autoantibody in acquired JMH-negative patients, which might further lead to the occurrence of haemolysis events. In this study, we found that the proportion of inherited JMH-negative people in the Guangzhou population was 0. Read More

Transfusion 2021 May 17. Epub 2021 May 17.

Children's National Hospital, Washington, DC, USA.

Background: Patients with sickle cell disease (SCD) are frequent recipients of red blood cell (RBC) transfusions and are at risk for RBC alloimmunization. RBC alloimmunization is diagnosed by identifying RBC alloantibodies as part of pre-transfusion testing, but this testing fails to detect alloantibodies that have evanesced. It may be beneficial to screen for new RBC alloantibody development after transfusion before possible antibody evanescence. Read More

Transfus Med Rev 2021 04 20;35(2):70-77. Epub 2021 Apr 20.

Univ Paris Est Creteil, INSERM, IMRB, Creteil, France; EFS Ile-de-France Créteil, Creteil, France; Laboratory of Excellence GR-Ex, Paris, France. Electronic address:

Hundreds of articles containing heterogeneous data describe D variants or add to the knowledge of known alleles. Data can be difficult to find despite existing online blood group resources and genetic and literature databases. We have developed a modern, elaborate database for D variants, thanks to an extensive literature search with meticulous curation of 387 peer-reviewed articles and 80 abstracts from major conferences and other sources. Read More

Transplant Cell Ther 2021 May 12. Epub 2021 May 12.

Japanese Data Center for Hematopoietic Cell Transplantation, Nagoya, Japan; Department of Healthcare Administration, Nagoya University Graduate School of Medicine, Nagoya, Japan.

Most patients of myelodysplastic syndrome (MDS) require red blood cell (RBC) or platelet transfusion during their disease courses, which could cause an increased risk of iron overload and alloimmunization. However, it remains less clear whether pretransplantation RBC or platelet transfusion burden affects transplant outcomes in patients with MDS. The objective was to examine the significance of pretransplantation RBC and platelet transfusion burden on transplant outcomes after allogeneic HCT for adults with de novo MDS. Read More

Transfusion 2021 May 13. Epub 2021 May 13.

Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, Washington, USA.

Background: Delayed hemolytic transfusion reactions (DHTRs) are reported to be rare occurrences but may be more frequently observed in the trauma setting where patients are heavily transfused, followed over long inpatient admissions, and have frequent subsequent blood counts as they undergo multiple surgical interventions.

Study Design And Methods: We examined the rates of DHTRs on a per transfusion and per patient basis in an academic county hospital with a level 1 trauma center serving a four-state region and over a 3-year period. DHTRs were entered sequentially into a registry as they were observed, and a retrospective review of all new alloantibodies detected was performed to identify any additional DHTRs. Read More

Mol Genet Genomic Med 2021 May 8:e1701. Epub 2021 May 8.

Blood transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.

Objectives: Serological methods may not be reliable for RBC antigen typing, especially in multi-transfused patients. The blood group systems provoking the most severe transfusion reactions are mainly Rh, Kell, Kidd, and Duffy. We intended to determine the genotype of these blood group system antigens among Iranian alloimmunized thalassemia patients using molecular methods and compare the results with serological phenotyping. Read More

Early Hum Dev 2021 Jun 4;157:105381. Epub 2021 May 4.

Department of Medical Psychology, Leiden University Medical Center, the Netherlands.

Aim: To investigate the school performance and behavioral difficulties in children with hemolytic disease of the fetus and newborn (HDFN) treated with intrauterine transfusion (IUT) compared to Dutch norm data.

Study Design: Cros-sectional cohort study.

Subjects: Children who received one or multiple IUTs for severe Rh- or K (Kell)-mediated HDFN between January 2008 and January 2015 at the LUMC. Read More

Transfusion 2021 May 7. Epub 2021 May 7.

Department of Pathology, University of California San Diego, La Jolla, California, USA.

Background: Daratumumab, a human anti-CD38 monoclonal antibody used to treat multiple myeloma, interferes with pretransfusion testing and can mask alloantibodies. Incidence of alloimmunization in patients on daratumumab has not been well characterized, and optimal transfusion guidelines regarding prophylactic antigen matching, accounting for both patient safety and efficiency, have not been well established for these patients.

Methods: Records of patients who received daratumumab between January 1, 2014 and July 2, 2019 were reviewed. Read More

Transfusion 2021 May 3. Epub 2021 May 3.

Department of Obstetrics, the Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Background: The anti-M antibody can lead to hemolytic disease of the fetus and newborn (HDFN) and adverse fetal outcomes, especially in the Asian population. However, fetal erythropoiesis resulting from M alloimmunization needs further investigation.

Study Design And Methods: We analyzed erythropoiesis in eight fetuses with M alloimmunization and compared them with the fetuses affected by anti-D. Read More

Transfus Clin Biol 2021 Apr 24. Epub 2021 Apr 24.

Hematology and Transfusion Medicine Center -University of Campinas/Hemocentro-Unicamp, Campinas, São Paulo, Brazil; Universidade São Francisco, Bragança Paulista, São Paulo, Brazil.

Objectives: Blood transfusion is a key intervention for decreasing morbidity and mortality in many cases and, besides its importance, potentially fatal consequences of incompatible transfusion are a great risk to patients. This study evaluated the incidence and specificity of erythrocyte alloantibodies in multi-transfused patients enrolled at an important Regional Blood Center.

Materials/methods: This was a single-center retrospective cohort study that eveluated patients enrolled at a Regional Blood Center in a period of four years. Read More

Blood 2021 Apr 19. Epub 2021 Apr 19.

Brigham and Women's Hosptial, Boston, Massachusetts, United States.

Red blood cell (RBC) transfusions can result in alloimmunization toward RBC alloantigens that can increase the probability of complications following subsequent transfusion. An improved understanding of the immune mechanisms that underlie RBC alloimmunization is critical if future strategies capable of preventing or even reducing this process are to be realized. Using the HOD (hen egg lysozyme and ovalbumin fused to human Duffy) model system, we aimed to identify initiating immune factors that may govern early anti-HOD alloantibody formation. Read More

Transfus Apher Sci 2021 Apr 8:103135. Epub 2021 Apr 8.

Center for Cell-Based Research, Regional Blood Center of Ribeirão Preto, School of Medicine of Ribeirão Preto, São Paulo, Brazil; University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.

Background: The correct determination of D antigen could help to avoid alloimmunization in pregnant women and patients receiving blood transfusions. However, there are limitations in the identification of D variants as the partial and weak D phenotypes make the determination of D antigen a great challenge in the transfusion routine.'

Study Design And Methods: The molecular characterization of D variants was performed on blood donors from southeastern Brazil with atypical D typing. Read More

Front Immunol 2021 30;12:657217. Epub 2021 Mar 30.

Aix Marseille Univ, CNRS, EFS, ADES, "Biologie des Groupes Sanguins", Marseille, France.

During pregnancy the formation of alloreactive anti-human leukocyte antigen (HLA) antibodies are a major cause of acute rejection in organ transplantation and of adverse effects in blood transfusion. The purpose of the study was to identify maternal HLA class Ib genetic factors associated with anti-HLA allo-immunization in pregnancy and the degree of tolerance estimated by IgG4 expression. In total, 86 primiparous women with singleton pregnancies were included in the study. Read More

Bioimpacts 2021 20;11(2):129-133. Epub 2020 Apr 20.

Departments of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Thalassemia is associated with a genetic decline in the rate of synthesis of one or more types of natural hemoglobin polypeptide chains. One of the major complications in thalassemia patients is alloimmunization, which is antibody production by the patient against transfused red blood cells (RBCs). These RBCs are unknown by the recipient and the formed antibodies against them are called alloantibodies. Read More

Transfus Apher Sci 2021 Apr 2:103124. Epub 2021 Apr 2.

Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran. Electronic address:

Background: Rhesus (Rh) blood group system is clinically the most significant protein-based grouping system. The Rh system is of vital importance in blood transfusion, and incompatibility between the donor and recipient leads to alloimmunization. Alloimmunization is commonly seen in multiple-transfusion recipients (e. Read More

Arch Gynecol Obstet 2021 Apr 9. Epub 2021 Apr 9.

Department of Blood Group Serology and Transfusion Medicine, Medical University of Vienna, Vienna, Austria.

Purpose: To evaluate the diagnostic accuracy of a commercially available test kit for noninvasive prenatal determination of the fetal RhD status (NIPT-RhD) with a focus on early gestation and multiple pregnancies.

Methods: The FetoGnost RhD assay (Ingenetix, Vienna, Austria) is routinely applied for clinical decision making either in woman with anti-D alloimmunization or to target the application of routine antenatal anti-D prophylaxis (RAADP) to women with a RhD positive fetus. Based on existing data in the laboratory information system the newborn's serological RhD status was compared with NIPT RhD results. Read More

Hematol Transfus Cell Ther 2021 Mar 6. Epub 2021 Mar 6.

Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.

Introduction: Platelet antibody identification is indispensable for diagnosing the human platelet antigen (HPA) or human leukocyte antigen (HLA) immunization, mostly because it can restrict the compatibility and results of transfusions. Correct detection of these antibodies is of utmost importance for the diagnosis and treatment.

Method: We present 16 platelet alloimmunization results, comparing two tests with different technologies: the MAIPA (monoclonal antibody immobilization of platelet antigens), as a reference technique, and a bead-based assay, the Pak-Lx. Read More

Transfusion 2021 Apr 3. Epub 2021 Apr 3.

Department of Fetal Medicine, Trousseau Hospital, Paris, France.

Background: Red blood cell alloimmunization is the first cause of fetal and neonatal anemia. Alloimmunizations with anti-PP1P or anti-P can cause recurrent miscarriages and hemolytic disease of the fetus and newborn in the 2nd and 3rd trimesters of pregnancy. We report on a pregnant patient immunized with anti-P and a history of recurrent miscarriages. Read More

Haematologica 2021 Apr 1. Epub 2021 Apr 1.

Department of Pediatrics, Children's Hospital of Philadelphia, Philadelphia, PA.

Red cell transfusion remains a critical component of care for acute and chronic complications of sickle cell disease. Randomized clinical trials demonstrated the benefits of transfusion therapy for prevention of primary and secondary strokes and postoperative acute chest syndrome. Transfusion for splenic sequestration, acute chest syndrome, and acute stroke are guided by expert consensus recommendations. Read More

Front Immunol 2021 12;12:629608. Epub 2021 Mar 12.

Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York, NY, United States.

Red blood cells expressing alloantigens are well known to be capable of inducing robust humoral alloantibody responses both in transfusion and pregnancy. However, the majority of transfusion recipients and pregnant women never make alloantibodies, even after repeat exposure to foreign RBCs. More recently, RBCs have been used as a cellular therapeutic-very much like transfusion, engineered RBCs are highly immunogenic in some cases but not others. Read More

Hematology 2021 Dec;26(1):321-327

Department of Pathology, University of Pittsburgh, Pittsburgh, PA, USA.

Objectives: To determine the rate of RhD-alloimmunization in injured RhD-negative patients in the age range of childbearing potential who were transfused with at least one unit of RhD-positive red blood cells (RBC) or low titer group O whole blood (LTOWB).

Methods: Injured RhD-negative patients between the ages of 13-50 at an American Level 1 trauma center who were transfused with at least one unit of RBCs or LTOWB during their resuscitation and who had an antibody detection test performed at least 14 days afterwards were included.

Results: Over a 20-year period, 96 study-eligible patients were identified, of which 90/96 (93. Read More

Hematol Transfus Cell Ther 2021 Feb 11. Epub 2021 Feb 11.

HEMOPA, Belém, PA, Brazil.

Introduction: Erythrocyte phenotyping is a very important test in the adoption of prophylactic measures to reduce transfusion reactions/alloimmunizations in polytransfused patients. The blood group Diego, in its current, form has 22 antigens, of which 4 are immunogenic, being Diª/Di and W/W, while the others are less expressive. The antigen Diª is of low incidence among whites and blacks, however, it is common in the South American indigenous and Asian Mongolian populations. Read More

Asian J Transfus Sci 2020 Jul-Dec;14(2):137-141. Epub 2020 Dec 19.

Department of Immunohematology and Blood Transfusion, Lady Hardinge Medical College, Connaught Place, New Delhi, India.

Background: Prevalence of rhesus (Rh) and Kell antigens in a population vary with race, ethnicity, and geographical location. With advances in immunohematology, non-D antigens, and their corresponding antibodies are increasingly being found to be culprits for alloimmunization.

Materials And Methods: Assessment of the phenotype of Rh and Kell blood group antigen in the healthy donor population from North India was done, and estimation of the frequencies of these alleles in our population was performed. Read More

Asian J Transfus Sci 2020 Jul-Dec;14(2):119-125. Epub 2020 Dec 19.

Department of Obstetrics and Gynaecology, Medical College, Kolkata, West Bengal, India.

Objectives: Detection of maternal irregular antibodies against red blood cell antigen is vital in the management of hemolytic disease of fetus and newborn. There are no uniform guidelines related to antenatal antibody screening and identification in the developing Country like India. This study was aimed to identify such alloimmunization and its associations. Read More

Transfusion 2021 Mar 21. Epub 2021 Mar 21.

Department of Laboratory Medicine, University of California San Francisco, San Francisco, California, USA.

Disparities are prevalent in numerous areas of healthcare. We sought to investigate whether there were racial/ethnic disparities among pregnant women with the most severe form of alloimmunization who require intrauterine transfusions (IUT). We reviewed patients who underwent IUT for alloimmunization at a single fetal treatment center between 2015 and 2020. Read More

J Gynecol Obstet Hum Reprod 2021 Mar 16;50(7):102119. Epub 2021 Mar 16.

Fetal Medicine Department, Armand-Trousseau Hospital, 26, Avenue du Dr Arnold Netter, 75012, Paris, France; Clinical Unit of CNRHP: Centre National de Référence en Hémobiologie Périnatale, Armand-Trousseau Hospital, 26, Avenue du Dr Arnold Netter, 75012, Paris, France; Sorbonne Université, Paris, France.

Background: Early intrauterine transfusion (IUT) is associated with a higher risk of fetal loss. Our objective was to evaluate the efficiciency of intravenous immunoglobulins (IVIG) to postpone the gestational age at first IUT beyond 20 weeks of gestation (WG) compared to the previous pregnancy in case of very severe red blood cell (RBC) alloimmunization.

Study Design And Methods: Very severe RBC alloimmunization was defined by a high titer of antibodies and a previous pregnancy complicated by a first IUT before 24 WG and/or perinatal death directly related to alloimmunization. Read More

Vox Sang 2021 03 17. Epub 2021 Mar 17.

Department of Paediatrics, Division of Neonatology, Leiden University Medical Centre, Leiden, the Netherlands.

Background And Objectives: Guidelines and indications for exchange transfusion in haemolytic disease of the foetus and newborn (HDFN) have changed drastically in the past decades, causing a decline in exchange transfusion rate. This study aims to evaluate the incidence of exchange transfusions (ETs) in neonates with Rh-mediated HDFN over the past 20 years at our centre, and report potentially ET-related complications as well as indicators for bilirubin encephalopathy.

Material And Methods: In this observational study, 438 neonates were included with HDFN, born ≥ 35 weeks gestational age at the Leiden University Medical Centre between January 2000 and July 2020. Read More

J Obstet Gynaecol Res 2021 Jun 15;47(6):2076-2081. Epub 2021 Mar 15.

Center for Maternal-Fetal, Neonatal and Reproductive Medicine, National Center for Child Health and Development, Tokyo, Japan.

Aim: The present study investigated the current situation regarding intrauterine blood transfusion (IUT) for fetal anemia in Japan.

Methods: We conducted a nationwide, multicenter, retrospective cohort questionnaire survey for cases that underwent IUT from 2011 to 2015. The questionnaire required perioperative information, indications, details of the procedure, procedure-related complications, and neonatal morbidity. Read More