Agric Sci China 2010 Jul 17;9(7):1050-1057. Epub 2010 Jul 17.
College of Animal Husbandry and Veterinary, Henan Agricultural University, Zhengzhou 450002, P.R. China.
A multiplex reverse transcriptase-polymerase chain reaction (multiplex RT-PCR) assay was developed and subsequently evaluated for its efficacy in the detection of multiple viral infections simultaneously, in swine. Specific primers for each of the 3 RNA viruses, North American genotype porcine reproductive and respiratory syndrome virus, Japanese encephalitis virus, and swine influenza virus, were used in the testing procedure. The assay was shown to be highly sensitive because it could detect as little as 10 ng of each of the respective amplicons in a single sample containing a composite of all 3 viruses. Read More