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Agric Sci China 2010 Jul 17;9(7):1050-1057. Epub 2010 Jul 17.

College of Animal Husbandry and Veterinary, Henan Agricultural University, Zhengzhou 450002, P.R. China.

A multiplex reverse transcriptase-polymerase chain reaction (multiplex RT-PCR) assay was developed and subsequently evaluated for its efficacy in the detection of multiple viral infections simultaneously, in swine. Specific primers for each of the 3 RNA viruses, North American genotype porcine reproductive and respiratory syndrome virus, Japanese encephalitis virus, and swine influenza virus, were used in the testing procedure. The assay was shown to be highly sensitive because it could detect as little as 10 ng of each of the respective amplicons in a single sample containing a composite of all 3 viruses. Read More

Agric Sci China 2009 Oct 28;8(10):1263-1269. Epub 2009 Oct 28.

College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, P.R. China.

The current research presents the protein changes in plasma from healthy dairy cows and clinical mastitic cows using two-dimensional gel electrophoresis (2-DE). After staining with silver nitrate and Coomassie Blue, differential expression proteins were detected by PDQuest 7.4 software, and then subjected to ion trap mass spectrometer equipped with a Surveyor HPLC System, differential spots of protein were identified. Read More

Agric Sci China 2009 Feb 24;8(2):231-237. Epub 2009 Feb 24.

College of Life Science and Technology, Heilongjiang August First Land Reclamation University, Daqing 163319, P.R. China.

Mucosal immunity plays an important role in protecting pigs against transmissible gastroenteritis virus (TGEV) infection. To elicit mucosal immune response against TGEV, we developed a surface antigen display system using the poly-[.gamma]-glutamate synthetase A (pgsA) protein of as an anchoring matrix to express recombinant fusion proteins of pgsA and nucleocapsid protein of TGEV in . Read More