Lab Chip 2021 Jun 14. Epub 2021 Jun 14.
Department of Mechanical and Aerospace Engineering, Monash University, Clayton, VIC 3800, Australia. and Centre to Impact Antimicrobial Resistance - Sustainable Solutions, Monash University, Clayton, 3800, Victoria, Australia and The Melbourne Centre for Nanofabrication, Victorian Node - Australian National Fabrication Facility, Clayton, Victoria 3800, Australia.
Volumetric, sub-micron to micron level resolution imaging is necessary to assay phenotypes or characteristics at the sub-cellular/organelle scale. However, three-dimensional fluorescence imaging of cells is typically low throughput or compromises on the achievable resolution in space and time. Here, we capitalise on the flow control capabilities of microfluidics and combine it with microoptics to integrate light-sheet based imaging directly into a microfluidic chip. Read More