5 results match your criteria Advances in microbiology[Journal]

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Characterizing Diversity of Lactobacilli Associated with Severe Early Childhood Caries: A Study Protocol.

Adv Microbiol 2015 Jan;5(1):9-20

Department of Cariology and Comprehensive Care, College of Dentistry, New York University, New York, USA.

Lactobacilli have been consistently associated with dental caries for decades; however, knowledge of this group of bacteria in the etiology of the disease is limited to quantitative elucidation. Nowadays, explicit identification of oral species is possible, despite their taxonomic complexity. Here we describe a combined approach involving both cultivation and genetic methods to ascertain and characterize the diversity and abundance of the population in the oral cavities of children with severe early childhood caries (S-ECC). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4583140PMC
January 2015
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Direct Detection and Quantification of Bacterial Genes Associated with Inflammation in DNA Isolated from Stool.

Adv Microbiol 2014 Nov;4(15):1065-1075

Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus, San Juan, Puerto Rico ; Molecular Sciences Building, University of Puerto Rico, Medical Sciences Campus, San Juan, Puerto Rico.

Although predominantly associated with health benefits, the gut microbiota has also been shown to harbor genes that promote inflammation. In this work, we report a method for the direct detection and quantification of these pro-inflammatory bacterial genes by PCR and qPCR in DNA extracted from human stool samples. PCR reactions were performed to detect (i) the island genes, (ii) , which is present in some strains of and (iii) presented in some strains of . Read More

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http://www.scirp.org/journal/doi.aspx?DOI=10.4236/aim.2014.4
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http://dx.doi.org/10.4236/aim.2014.415117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307837PMC
November 2014
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The LysR Transcription Factor, HexS, Is Required for Glucose Inhibition of Prodigiosin Production by

Adv Microbiol 2012 Dec;2(4)

Charles T. Campbell Laboratory of Ophthalmic Microbiology, Department of Ophthalmology, University of Pittsburgh, Pittsburgh, USA.

Generation of many useful microbe-derived secondary metabolites, including the red pigment prodigiosin of the bacterium , is inhibited by glucose. In a previous report, a genetic approach was used to determine that glucose dehydrogenase activity (GDH) is required for inhibiting prodigiosin production and transcription of the prodigiosin biosynthetic operon (). However, the transcription factor(s) that regulate this process were not characterized. Read More

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http://dx.doi.org/10.4236/aim.2012.24065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3865871PMC
December 2012
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Description of a Putative Oligosaccharyl:S-Layer Protein Transferase from the Tyrosine -Glycosylation System of CCM 2051.

Adv Microbiol 2012 Dec;2(4):537-546

Department of NanoBiotechnology, NanoGlycobiology Unit, Universität für Bodenkultur Wien, Wien, Austria.

Surface (S)-layer proteins are model systems for studying protein glycosylation in bacteria and simultaneously hold promises for the design of novel, glyco-functionalized modules for nanobiotechnology due to their 2D self-assembly capability. Understanding the mechanism governing S-layer glycan biosynthesis in the Gram-positive bacterium CCM 2051 is necessary for the tailored glyco-functionalization of its S-layer. Here, the putative oligosaccharyl:S-layer protein transferase WsfB from the S-layer glycosylation gene locus is characterized. Read More

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http://dx.doi.org/10.4236/aim.2012.24069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4397953PMC
December 2012
32 Reads

Differential Role of Two-Component Regulatory Systems ( and ) in Polymyxin B Susceptibility of

Adv Microbiol 2012 Mar;2(1)

Department of Biology, Long Island University, New York, USA ; Department of Medicine of Michael E DeBakey VA Medical Center, Baylor College of Medicine, Houston, USA.

Polymyxins are often considered as a last resort to treat multidrug resistant but polymyxin resistance has been increasingly reported worldwide in clinical isolates. Polymyxin resistance in is known to be associated with alterations in either PhoQ or PmrB. In this study, mutant strains of carrying amino acid substitution, a single and/or dual inactivation of PhoQ and PmrB were constructed to further understand the roles of PhoQ and PmrB in polymyxin susceptibility. Read More

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http://dx.doi.org/10.4236/aim.2012.21005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3859615PMC
March 2012
2 Reads
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