18 results match your criteria Advances in bioscience and biotechnology Print[Journal]

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TALEN-Mediated FLAG-Tagging of Endogenous Histone Methyltransferase DOT1L.

Adv Biosci Biotechnol 2017 Sep 22;8(9):311-323. Epub 2017 Sep 22.

Laboratory of Human Environmental Epigenomes, Department of Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA.

Histone modification including H3 lysine 79 methylation (H3K79me) plays a key role during gene transcription and DNA damage repair. DOT1L, the sole methyltransferase for three states of H3K79me, is implicated in leukemia, co-lorectal cancer, and dilated cardiomyopathy. However, understanding of DOT1L and H3K79me in these pathways and disease pathogenesis has been limited due to the difficulty of working with DOT1L protein. Read More

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http://dx.doi.org/10.4236/abb.2017.89023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5963693PMC
September 2017
2 Reads

The Role of DNA Mismatch Repair and Recombination in the Processing of DNA Alkylating Damage in Living Yeast Cells.

Adv Biosci Biotechnol 2015 1;6(6):408-418. Epub 2015 Jun 1.

Institute of Molecular Medicine and Genetics, Georgia Regents University, Augusta, USA.

It is proposed that mismatch repair (MMR) mediates the cytotoxic effects of DNA damaging agents by exerting a futile repair pathway which leads to double strand breaks (DSBs). Previous reports indicate that the sensitivity of cells defective in homologous recombination (HR) to DNA alkylation is reduced by defects in MMR genes. We have assessed the contribution of different MMR genes to the processing of alkylation damage . Read More

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http://dx.doi.org/10.4236/abb.2015.66040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4758339PMC
June 2015
10 Reads

Single Nucleotide Polymorphisms: A Window into the Informatics of the Living Genome.

Adv Biosci Biotechnol 2014 Jun;5(7):623-626

Computational Physics Laboratory, Howard University, Washington DC, USA.

Nested in the environment of the nucleus of the cell, the 23 sets of chromosomes that comprise the human genome function as one integrated whole system, orchestrating the expression of thousands of genes underlying the biological characteristics of the cell, individual and the species. The extraction of meaningful information from this complex data set depends crucially upon the lens through which the data are examined. We present a biophysical perspective on genomic information encoded in single nucleotide polymorphisms (SNPs), and introduce metrics for modeling information encoded in the genome. Read More

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http://dx.doi.org/10.4236/abb.2014.57073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307840PMC
June 2014
1 Read

LL-37 induced cystitis and the receptor for advanced glycation end-products (RAGE) pathway.

Adv Biosci Biotechnol 2013 Aug;4(8B):1-8

Department of Surgery and Division of Pediatric Urology: Primary Children's Medical Center, Salt Lake City, Utah.

To elucidate pathways in bladder inflammation, we employed our physiologically relevant LL-37 induced cystitis model. Based on inflammatory studies involving other organ systems implicating the receptor for advanced glycation end-products (RAGE), we first hypothesized that RAGE is critically involved in LL-37 induced cystitis. We further hypothesized a common RAGE ligand - high mobility group box 1 (HMGB1) is up-regulated in bladders challenged with LL-37. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4039633PMC
August 2013
1 Read

Evaluation of HemogloBind™ treatment for preparation of samples for cholinesterase analysis.

Adv Biosci Biotechnol 2013 Dec;4(12):1020-1023

Battelle Biomedical Research Center, Battelle Memorial Institute, Columbus, USA.

Acetylcholine is an essential neurotransmitter found throughout the nervous system. Its action on postsynaptic receptors is regulated through hydrolysis by various carboxylesterases, especially cholinesterases (ChEs). The acute toxicity of organophosphate (OP) compounds is directly linked to their action as inhibitors of ChE. Read More

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http://dx.doi.org/10.4236/abb.2013.412136DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989896PMC
December 2013
8 Reads
4 Citations

Synergistic interaction between C5a and NOD2 signaling in the regulation of chemokine expression in RAW 264.7 macrophages.

Adv Biosci Biotechnol 2013 Aug;4(8C):30-37

Departments of Pathobiology Cleveland Clinic, Cleveland, USA ; Department of Molecular Medicine, Case Western Reserve University, Cleveland, USA ; Department of Gastroenterology Cleveland Clinic, Cleveland, USA.

The innate immune response is a complex process involving multiple pathogen-recognition receptors, including toll-like receptors (TLRs) and nucleotide-binding oligomerization domain (NOD)-like receptors. Complement is also a critical component of innate immunity. While complement is known to interact with TLR-mediated signals, the interactions between NOD-like receptors and complement are not well understood. Read More

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http://www.scirp.org/journal/doi.aspx?DOI=10.4236/abb.2013.4
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http://dx.doi.org/10.4236/abb.2013.48A3004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3952140PMC
August 2013
3 Reads

Aging promotes pro-fibrotic matrix production and increases fibrocyte recruitment during acute lung injury.

Adv Biosci Biotechnol 2014 Jan;5(1):19-30

Division of Pulmonary, Allergy and Critical Care Medicine, Emory University School of Medicine, Atlanta, GA, USA ; Division of Pulmonary, Allergy and Critical Care Medicine, Atlanta VAMC, Decatur, GA, USA.

Fibrotic lung diseases increase with age. Previously we determined that senescence increases tissue expression of fibronectin EDA (Fn-EDA) and decreases fibroblast expression of Thy-1, and that fibrocytes contribute to fibrosis following bleomycin-induced lung injury in mice. In this study we hypothesized that fibroblasts lacking Thy-1 expression produce an extracellular matrix that promotes fibrocyte retention and myofibroblast transdifferentiation, thereby promoting fibrogenesis. Read More

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http://www.scirp.org/journal/doi.aspx?DOI=10.4236/abb.2014.5
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http://dx.doi.org/10.4236/abb.2014.51004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3939026PMC
January 2014
10 Reads

Role of TGF- in breast cancer bone metastases.

Adv Biosci Biotechnol 2013 Oct;4(10C):15-30

Division of Endocrinology, Department of Internal Medicine, Indiana University, Indianapolis, USA.

Breast cancer is the most prevalent cancer among females worldwide leading to approximately 350,000 deaths each year. It has long been known that cancers preferentially metastasize to particular organs, and bone metastases occur in ~70% of patients with advanced breast cancer. Breast cancer bone metastases are predominantly osteolytic and accompanied by increased fracture risk, pain, nerve compression and hypercalcemia, causing severe morbidity. Read More

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http://dx.doi.org/10.4236/abb.2013.410A4003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3928102PMC
October 2013
5 Reads

Cyclophosphamide Induces an Early Wave of Acrolein-Independent Apoptosis in the Urothelium.

Adv Biosci Biotechnol 2013 Aug;4(88)

Department of Urology, Medical University of South Carolina. Charleston, SC ; Department of Pediatrics, Medical University of South Carolina. Charleston, SC ; Department of Regenerative Medicine and Cell Biology. Medical University of South Carolina. Charleston, SC.

Purpose: Hemorrhagic cystitis (HC or bladder inflammation) affects a significant number of patients undergoing cyclophosphamide (CP) chemotherapy despite treatment with 2-mercaptothane ulfonate (Mesna) to inactivate the metabolite acrolein. While the mechanism is unknown, there is clearly acrolein-independent damage to the urothelium. In this study we have explored the induction of apoptosis in the urothelium as a marker of damage and the mechanism underlying the acrolein-independent apoptosis. Read More

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http://dx.doi.org/10.4236/abb.2013.48A2002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3864667PMC
August 2013
3 Reads

Physiological effects of nickel chloride on the freshwater cyanobacterium sp. IU 625.

Adv Biosci Biotechnol 2013 Jul;4(7B):10-14

Department of Biological Sciences, Seton Hall University, South Orange, USA.

Harmful algal blooms (HABs) are a serious environmental problem globally. The ability of cyanobacteria, one of the major causative agents of HABs, to grow in heavy metal polluted areas is proving a challenge to environmental restoration initiatives. Some cyanobacteria secrete toxins, such as microcystin, that are potentially dangerous to animals and humans. Read More

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http://dx.doi.org/10.4236/abb.2013.47A2002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782114PMC
July 2013
15 Reads

Strategies to stabilize compact folding and minimize aggregation of antibody-based fragments.

Adv Biosci Biotechnol 2013 Apr;4(4a):73-84

Department of Immunology, Mayo Clinic College of Medicine, Rochester, USA.

Monoclonal antibodies (mAbs) have proven to be useful for development of new therapeutic drugs and diagnostic techniques. To overcome the difficulties posed by their complex structure and folding, reduce undesired immunogenicity, and improve pharmacokinetic properties, a plethora of different Ab fragments have been developed. These include recombinant Fab and Fv segments that can display improved properties over those of the original mAbs upon which they are based. Read More

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http://dx.doi.org/10.4236/abb.2013.44A011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307952PMC
April 2013
2 Reads

Increased survivin expression contributes to apoptosis-resistance in IPF fibroblasts.

Adv Biosci Biotechnol 2012 Oct;3(6A):657-664

Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Michigan Medical Center, Ann Arbor, USA.

Fibroblasts perform critical functions during the normal host response to tissue injury, but the inappropriate accumulation and persistent activation of these cells results in the development of tissue fibrosis. The mechanisms accounting for the aberrant accumulation of fibroblasts during fibrotic repair are poorly understood, although evidence supports a role for fibroblast resistance to apoptosis as a contributing factor. We have shown that TGF-β1 and endothelin-1 (ET-1), soluble mediators implicated in fibrogenesis, promote fibroblast resistance to apoptosis. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553664PMC
October 2012
28 Reads

Increased resistance to apoptosis during differentiation and syncytialization of BeWo choriocarcinoma cells.

Adv Biosci Biotechnol 2012 Oct 30;3(6A):805-813. Epub 2012 Oct 30.

Department of Reproductive Biology, Case Western Reserve University School of Medicine, Case Western Reserve University, Cleveland, USA.

Transition from mononuclear villous cytotrophoblast into multinuclear syncytiotrophoblast in the human placenta is accompanied by changes in apoptosis-related proteins and an apparent increased resistance to induced apoptosis. We investigated the specific nature and timing of changes in Bcl-2, Bax, p53, and caspases 3 and 8 in forskolin-treated BeWo choriocarcinoma cells, a model for villous cytotrophoblast differentiation. BeWo cells were treated with forskolin or vehicle alone for up to 72 h and evaluated at 24 h intervals for syncytialization and quantitative expression specific apoptosis-related proteins and mRNAs. Read More

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http://dx.doi.org/10.4236/abb.2012.326100DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5881895PMC
October 2012

Vitamin C activation of the biosynthesis of epoxyeicosatrienoic acids.

Adv Biosci Biotechnol 2012 Jun;3(3):204-218

Department of Pharmacology, New York Medical College, Valhalla, New York, USA.

The cardiovascular effects of vitamin C (VitC) could be mediated by epoxyeicosatrienoic acids (EETs). We aimed to study the mechanism of VitC-dependent microsomal formation of - and -EETs and the regulation of EET levels in rat isolated perfused kidneys and . VitC biphasically stimulated rat kidney microsomal - and -EET formation in a ratio of 1:2, involving the participation of lipid hydroperoxides (LOOHs), Fe, and cytochrome P450 (CYP). Read More

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http://dx.doi.org/10.4236/abb.2012.33029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958942PMC
June 2012
18 Reads

Degradation of naturally occurring and engineered antimicrobial peptides by proteases.

Adv Biosci Biotechnol 2011 Dec;2(6):404-408

Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, Pittsburgh, USA.

We hypothesized that current antimicrobial peptides should be susceptible to proteolytic digestion. The antimicrobial peptides: Griffithinsin, RC-101, LL-37, LSA-5, PSC-RANTES and DJ007 were degraded by commercially available proteases. Two different species of anaerobic vaginal flora, Prevotella bivia and Porphyromonas asaccharolytica also degraded the materials. Read More

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http://dx.doi.org/10.4236/abb.2011.26059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3354962PMC
December 2011
1 Read

Nitric oxide leads to cytoskeletal reorganization in the retinal pigment epithelium under oxidative stress.

Adv Biosci Biotechnol 2012 ;3:1167-1178

Department of Biological Sciences, Michigan Technological University, Houghton, USA.

Light is a risk factor for various eye diseases, including age-related macular degeneration (AMD) and retinitis pigmentosa (RP). We aim to understand how cytoskeletal proteins in the retinal pigment epithetlium (RPE) respond to oxidative stress, including light and how these responses affect apoptotic signaling. Previously, proteomic analysis revealed that the expression levels of vimentin and serine/threonine protein phosphatase 2A (PP2A) are significantly increased when mice are exposed under continuous light for 7 days compared to a condition of 12 hrs light/dark cycling exposure using retina degeneration 1 (rd1) model. Read More

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http://dx.doi.org/10.4236/abb.2012.38143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5152770PMC
January 2012
7 Reads

pHluorin2: an enhanced, ratiometric, pH-sensitive green florescent protein.

Authors:
Matthew J Mahon

Adv Biosci Biotechnol 2011 Jun;2(3):132-137

Endocrine Unit, Massachusetts General Hospital and Department of Medicine, Harvard Medical School.

Green florescent protein (GFP) variants that are sensitive to changes in pH are invaluable reagents for the analysis of protein dynamics associated with both endo- and exocytotic vesicular trafficking. Ratiometric pHluorin is a GFP variant that displays a bimodal excitation spectrum with peaks at 395 and 475 nm and an emission maximum at 509 nm. Upon acidification, pHluorin excitation at 395 nm decreases with a corresponding increase in the excitation at 475 nm. Read More

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http://dx.doi.org/10.4236/abb.2011.23021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3152828PMC

Direct measurement of oxygen consumption rates from attached and unattached cells in a reversibly sealed, diffusionally isolated sample chamber.

Adv Biosci Biotechnol 2010 Dec;5(5):398-408

Department of Electrical Engineering, University of Washington, Seattle, USA.

Oxygen consumption is a fundamental component of metabolic networks, mitochondrial function, and global carbon cycling. To date there is no method available that allows for replicate measurements on attached and unattached biological samples without compensation for extraneous oxygen leaking into the system. Here we present the Respiratory Detection System, which is compatible with virtually any biological sample. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3086294PMC
December 2010
13 Reads
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