226,419 results match your criteria Advances in Nursing Science ANS[Journal]


Genome mining and prospects for antibiotic discovery.

Authors:
Lucy Foulston

Curr Opin Microbiol 2019 Feb 15;51:1-8. Epub 2019 Feb 15.

Warp Drive Bio, A Subsidiary of Revolution Medicines, Inc., 400 Technology Square, Cambridge, MA 02139, USA.

Natural products are a rich source of bioactive compounds that have been used successfully in the areas of human health from infectious disease to cancer; however, traditional fermentation-based screening has provided diminishing returns over the last 20-30 years. Solutions to the unmet need of resistant bacterial infection are critically required. Technological advances in high-throughput genomic sequencing, coupled with ever-decreasing cost, are now presenting a unique opportunity for the reinvigoration of natural product discovery. Read More

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http://dx.doi.org/10.1016/j.mib.2019.01.001DOI Listing
February 2019

Using mouse genetics to understand human skeletal disease.

Bone 2019 Feb 15. Epub 2019 Feb 15.

Division of Bone Biology, Garvan Institute of Medical Research, Sydney, NSW 2010, Australia; St Vincent's Clinical School, Faculty of Medicine, UNSW Australia, Sydney, NSW, 2010, Australia; University of Notre Dame Australia, Sydney, NSW, 2010, Australia.

Technological advances have enabled the study of the human genome in incredible detail with relative ease. However, our ability to interpret the functional significance of the millions of genetic variants present within each individual is limited. As a result, the confident assignment of disease-causing variant calls remains a significant challenge. Read More

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http://dx.doi.org/10.1016/j.bone.2019.02.015DOI Listing
February 2019

Nicotinamide Metabolism Modulates the Proliferation / Differentiation Balance and Senescence of Human Primary Keratinocytes.

J Invest Dermatol 2019 Feb 15. Epub 2019 Feb 15.

Cell Cycle Control in Skin Epidermis, Skin Research Institute of Singapore, A*STAR, 8A Biomedical Grove, Singapore 138648; School of Biological Sciences & Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 637551. Electronic address:

Nicotinamide (NAM) is the main precursor of NAD, a co-enzyme essential for DNA repair, glycolysis and oxidative phosphorylation (OXPHOS). NAM has anti-aging activity on human skin, but the underlying mechanisms of action are unclear. Using 3D organotypic skin models, we show that NAM inhibits differentiation of the upper epidermal layers and maintains proliferation in the basal layer. Read More

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http://dx.doi.org/10.1016/j.jid.2019.02.005DOI Listing
February 2019

Current concepts in severe acute and necrotizing pancreatitis: an evidence-based approach.

Gastroenterology 2019 Feb 15. Epub 2019 Feb 15.

Department of Surgery, Amsterdam Gastroenterology and Metabolism, Amsterdam UMC, University of Amsterdam, the Netherlands.

The incidence of acute pancreatitis continues to rise, inducing substantial medical and social burden, with annual costs exceeding two billion dollars in the USA alone. Although the majority of patients develop mild pancreatitis, 20% develop severe and/or necrotizing pancreatitis, requiring advanced medical and interventional care. Morbidity resulting from local and systemic complications as well as invasive interventions result in mortality rates historically as high as 30%. Read More

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http://dx.doi.org/10.1053/j.gastro.2019.01.269DOI Listing
February 2019

Early intra-acinar events in Pathogenesis of Pancreatitis.

Gastroenterology 2019 Feb 15. Epub 2019 Feb 15.

Minnesota Gastroenterology, Minneapolis, MN 55433.

Premature activation of digestive enzymes in the pancreas has been linked to development of pancreatitis for more than a century. Recent development of novel models to study the role of pathologic enzyme activation has led to advances in our understanding of the mechanisms of pancreatic injury. Co-localization of zymogen and lysosomal fraction occurs early after pancreatitis causing stimulus. Read More

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http://dx.doi.org/10.1053/j.gastro.2019.01.268DOI Listing
February 2019

[Use of virtual reality for neurodevelopmental disorders. A review of the state of the art and future agenda].

Medicina (B Aires) 2019 ;79 Suppl 1:77-81

Centro de Desarrollo Cognitivo Red Cenit, Valencia, España.

To date, the diagnostic tools for autism spectrum disorder (ASD) have been mostly based on qualitative criteria from observational information in contexts with low ecological validity. We are witnessing a growing scientific activity that proposes the use of implicit measures for the evaluation and diagnosis of ASD. These measures are based on processes of a biological and unconscious nature, underlying the capacity of human cognition, and are obtained through the acquisition and treatment of brain, physiological and behavioral responses in order to obtain the behavioral structure of the ASD patient facing a stimulus. Read More

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January 2019

[Neurobiological bases of autism and cellular models for its experimental study].

Medicina (B Aires) 2019 ;79 Suppl 1:27-32

Intituto de Neurociencias UMH-CSIC. San Joan d'Alacant. Alicante. España. E-mail:

Autism Spectrum Disorders (ASD) are a functional alteration of the cerebral cortex, which presents structural neurodevelopmental anomalies that affect synaptic function and the pattern of connections within and between cortical columns. From its etiological aspect, ASD has an important genetic load, considering a polygenic disorder, derived from a combination of "de novo" genetic mutations, associated to a predisposition derived from common inherited variations. The main genetic anomalies associated with ASD involve genes that encode proteins of the synapse. Read More

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January 2019

[Autism. Genetic and biological aspects].

Medicina (B Aires) 2019 ;79 Suppl 1:16-21

Hospital de Pediatría Prof. Dr. Juan P. Garrahan, Buenos Aires, Argentina.

Autism is a neurodevelopmental disorder characterized by commitment to social interaction and communication, associated with interests restricted and stereotyped behaviors with a high population prevalence, neurobiological bases and high heritability. Its etiology is heterogeneous, numerous genetic bases, environmental factors and epigenetic mechanisms have been recognized. Advances in molecular genetics, as well as epidemiological studies of large cohorts, have made it possible to identify specific medical entities, as well as genes and environmental factors partially or totally linked in their pathogenesis. Read More

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January 2019

Unraveling the host's immune response to infection: Seeing is believing.

J Leukoc Biol 2019 Feb 18. Epub 2019 Feb 18.

University of Calgary Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta, Canada.

It has long been appreciated that understanding the interactions between the host and the pathogens that make us sick is critical for the prevention and treatment of disease. As antibiotics become increasingly ineffective, targeting the host and specific bacterial evasion mechanisms are becoming novel therapeutic approaches. The technology used to understand host-pathogen interactions has dramatically advanced over the last century. Read More

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http://dx.doi.org/10.1002/JLB.4RI1218-503RDOI Listing
February 2019

Danger signals in oral cavity-related diseases.

J Leukoc Biol 2019 Feb 18. Epub 2019 Feb 18.

Department of Oral Biology, School of Dental Medicine, University at Buffalo, Buffalo, New York, USA.

The oral cavity is a unique environment containing teeth juxtaposed with soft tissues, all of which are constantly bathed in microbial products and host-derived factors. While microbial dysbiosis in the oral cavity clearly leads to oral inflammatory disease, recent advances find that endogenous danger-associated molecular patterns (DAMPs) released from oral and salivary tissue also contribute to the progression of inflammatory and autoimmune disease, respectively. In contrast, DAMPs produced during oral fungal infection actually promote the resolution of infection. Read More

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http://dx.doi.org/10.1002/JLB.4MIR1118-439RDOI Listing
February 2019

A novel technique for in situ uniaxial tests of self-assembled soft biomaterials.

Lab Chip 2019 Feb 18. Epub 2019 Feb 18.

University of Illinois at Urbana-Champaign, 1206 W. Green St, Urbana, IL 61801, USA.

We introduce a novel method to form 3D biomimetic tissues from a droplet of a cell-extracellular matrix (ECM) mixture on a sensor stage and to quantify tissue force and stiffness as a function of time under optical microscopes. This method exploits advances in micro-nano fabrication and capillarity for self-assembly and self-alignment of tissues on the stage. It allows simultaneous investigation of the microstructure of the tissue in situ while its mechanical response is quantified, thus linking tissue biophysics with physiology and revealing structural-functional properties of 3D tissues. Read More

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http://dx.doi.org/10.1039/c8lc01273cDOI Listing
February 2019

A promising approach to targeting type 1 IFN in systemic lupus erythematosus.

J Clin Invest 2019 Feb 18. Epub 2019 Feb 18.

Division of Rheumatology, Cedars-Sinai Medical Center, David Geffen School of Medicine, UCLA, Los Angeles, California, USA.

Despite advances in understanding systemic lupus erythematosus (SLE) pathogenesis, most clinical trials of new targeted therapies have been met with disappointment. The type I IFN pathway is believed to play an important role in SLE, and the proposed involvement of this pathway helps explain the frustration behind the failure at targeting either IFN-α or the type 1 IFN receptor itself. In this issue of the JCI, Furie et al. Read More

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http://dx.doi.org/10.1172/JCI127101DOI Listing
February 2019

A phonological assessment test for child Greek.

Authors:
Elena Babatsouli

Clin Linguist Phon 2019 Feb 18:1-27. Epub 2019 Feb 18.

a Institute of Monolingual and Bilingual Speech , Chania , Greece.

This article advances a clinical tool for assessing typical and atypical phonological development in children speaking standard Modern Greek or Hellenic (ISO 639.1, el). The proposed tool develops a comprehensive test that is archetypical of the standard idiom and of predominant dialectal variations and seeks to be readily available for logopedics and language researchers of child Greek anywhere. Read More

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http://dx.doi.org/10.1080/02699206.2019.1569164DOI Listing
February 2019

European Respiratory Society International Congress, Paris, 2018: highlights from the Clinical Assembly.

ERJ Open Res 2019 Feb 11;5(1). Epub 2019 Feb 11.

Dept of Pneumology and Critical Care Medicine, Thoraxklinik, Heidelberg University Hospital, Heidelberg, Germany.

This article contains highlights and a selection of the scientific advances from the European Respiratory Society's Clinical Assembly (Assembly 1 and its five respective groups) that were presented at the 2018 European Respiratory Society International Congress in Paris, France. The most relevant topics from each of the groups will be discussed, covering a wide range of areas including clinical problems, rehabilitation and chronic care, thoracic imaging, interventional pulmonology, and general practice and primary care. The newest research, actual data and highlight sessions will be discussed. Read More

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http://dx.doi.org/10.1183/23120541.00176-2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368995PMC
February 2019

Rhoptry antigens as vaccine target.

Clin Exp Vaccine Res 2019 Jan 31;8(1):4-26. Epub 2019 Jan 31.

Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran.

Toxoplasmosis is a cosmopolitan zoonotic infection, caused by a unicellular protozoan parasite known as that belongs to the phylum Apicomplexa. It is estimated that over one-third of the world's population has been exposed and are latently infected with the parasite. In humans, toxoplasmosis is predominantly asymptomatic in immunocompetent persons, while among immunocompromised individuals may be cause severe and progressive complications with poor prognosis. Read More

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http://dx.doi.org/10.7774/cevr.2019.8.1.4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369123PMC
January 2019

Controlled dissolution of freeform 3D printed carbohydrate glass scaffolds in hydrogels using a hydrophobic spray coating.

Addit Manuf 2019 Mar 27;26:193-201. Epub 2018 Dec 27.

Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL.

Freeform 3D printing combined with sacrificial molding promises to lead advances in production of highly complex tubular systems for biomedical applications. Here we leverage a purpose-built isomalt 3D printer to generate complex channel geometries in hydrogels which would be inaccessible with other techniques. To control the dissolution of the scaffold, we propose an enabling technology consisting of an automated nebulizer coating system which applies octadecane to isomalt scaffolds. Read More

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http://dx.doi.org/10.1016/j.addma.2018.12.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6371974PMC

Targeting androgen receptor-independent pathways in therapy-resistant prostate cancer.

Asian J Urol 2019 Jan 28;6(1):91-98. Epub 2018 Nov 28.

Department of Pathology, Duke University School of Medicine, Durham, NC, USA.

Since androgen receptor (AR) signaling is critically required for the development of prostate cancer (PCa), targeting AR axis has been the standard treatment of choice for advanced and metastatic PCa. Unfortunately, although the tumor initially responds to the therapy, treatment resistance eventually develops and the disease will progress. It is therefore imperative to identify the mechanisms of therapeutic resistance and novel molecular targets that are independent of AR signaling. Read More

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http://dx.doi.org/10.1016/j.ajur.2018.11.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6363598PMC
January 2019

Metastatic prostate cancer remains incurable, why?

Asian J Urol 2019 Jan 29;6(1):26-41. Epub 2018 Nov 29.

The Brady Urological Institute, Johns Hopkins School of Medicine, Baltimore, MD, USA.

Metastatic prostate cancer patients present in two ways-with already disseminated disease at the time of presentation or with disease recurrence after definitive local therapy. Androgen deprivation therapy is given as the most effective initial treatment to patients. However, after the initial response, almost all patients will eventually progress despite the low levels of testosterone. Read More

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http://dx.doi.org/10.1016/j.ajur.2018.11.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6363601PMC
January 2019

Advances in immune checkpoint inhibitors for bone sarcoma therapy.

J Bone Oncol 2019 Apr 29;15:100221. Epub 2019 Jan 29.

Department of Orthopedic Surgery, Sarcoma Biology Laboratory, David Geffen School of Medicine, University of California, 615 Charles E. Young. Dr. South, Los Angeles, CA 90095, USA.

Bone sarcomas are a collection of sporadic malignancies of mesenchymal origin. The most common subtypes include osteosarcoma, Ewing sarcoma, chondrosarcoma, and chordoma. Despite the use of aggressive treatment protocols consisting of extensive surgical resection, chemotherapy, and radiotherapy, outcomes have not significantly improved over the past few decades for osteosarcoma or Ewing sarcoma patients. Read More

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http://dx.doi.org/10.1016/j.jbo.2019.100221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365405PMC

CAR-T immunotherapy: how will it change treatment for acute lymphoblastic leukemia and beyond?

Expert Opin Orphan Drugs 2018 8;6(10):563-566. Epub 2018 Oct 8.

Department of Pediatrics and Carbone Comprehensive Cancer Center, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.

Introduction: The recent approval of CD19 chimeric antigen receptor (CAR) T cells for refractory or second relapse of B cell acute lymphoblastic leukemia (B-ALL) has led to a paradigm shift. Besides being an alternative to chemotherapy and antibody-based approaches, CAR-T cells have become the first successful example of "personalized medicine."

Areas Covered: In clinical trials, tisagenlecleucel demonstrated higher response rates than prior therapies, and led to durable remissions lasting up to years for some children. Read More

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http://dx.doi.org/10.1080/21678707.2018.1529562DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375487PMC
October 2018

Enduring Clinical Value of Copaxone® (Glatiramer Acetate) in Multiple Sclerosis after 20 Years of Use.

Authors:
Daniel R Wynn

Mult Scler Int 2019 15;2019:7151685. Epub 2019 Jan 15.

Director, Clinical Research; Consultants in Neurology, Multiple Sclerosis Center, Northbrook, IL, USA.

Multiple sclerosis (MS) is a chronic progressive neurodegenerative demyelinating disease affecting the central nervous system. Glatiramer acetate (GA; Copaxone®) was the first disease-modifying treatment (DMT) for MS successfully tested in humans (1977) and was approved by the US Food and Drug Administration in December 1996. Since then, there have been numerous developments in the MS field: advances in neuroimaging allowing more rapid and accurate diagnosis; the availability of a range of DMTs including immunosuppressant monoclonal antibodies and oral agents; a more holistic approach to treatment by multidisciplinary teams; and an improved awareness of the need to consider a patient's preferences and patient-reported outcomes such as quality of life. Read More

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http://dx.doi.org/10.1155/2019/7151685DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350531PMC
January 2019

Circulating free tumor DNA in non-small cell lung cancer (NSCLC): clinical application and future perspectives.

J Thorac Dis 2019 Jan;11(Suppl 1):S113-S126

Department of Biochemistry, Nantes University Hospital, 9 quai Moncousu, F-44093 Nantes Cedex, France.

Major advances in the treatment of non-small cell lung cancer (NSCLC) patients have been obtained during the last decade. Molecular testing of tumor samples is therefore mandatory in routine clinical practice. Tumor DNA is also present as cell-free molecules in blood, which is therefore a very useful and convenient source of tumor DNA. Read More

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http://dx.doi.org/10.21037/jtd.2018.12.18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353745PMC
January 2019

Are there any theranostic biomarkers in small cell lung carcinoma?

J Thorac Dis 2019 Jan;11(Suppl 1):S102-S112

Department of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova Medical School, Padova, Italy.

Small cell lung cancer (SCLC), an aggressive lung tumour with a poor prognosis, has a high load of somatic mutations, mainly induced by tobacco carcinogens given the strong association with smoking. Advances in genomic, epigenetic and proteomic profiling have significantly improved our understanding of the molecular and cellular biology of SCLC. Given the high mutational burden of SCLC the immune microenvironment is another exciting area under investigation even if it seems to be quite distinct from that of other solid tumours. Read More

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http://dx.doi.org/10.21037/jtd.2018.12.14DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353747PMC
January 2019

Current views on tumor mutational burden in patients with non-small cell lung cancer treated by immune checkpoint inhibitors.

J Thorac Dis 2019 Jan;11(Suppl 1):S71-S80

Laboratory of Clinical and Experimental Pathology, Pasteur Hospital, University Hospital Federation OncoAge, Antoine Lacassagne Comprehensive Cancer Center, Université Côte d'Azur, Nice, France.

In the last few years, the treatment of patients with non-small cell lung cancer (NSCLC) has impressively benefitted from immunotherapy, in particular from the inhibition of immune checkpoints such as programmed cell death-1 (PD-1) and programmed cell death ligand-1 (PD-L1). However, despite the significant survival benefit for some patients with advanced NSCLC, the objective response rates (ORRs) remain relatively low no more than 20-30% with a large proportion of patients demonstrating primary resistance. Although the selection of NSCLC patients for the first-line treatment is currently guided by the expression of PD-L1 in tumor cells as detected by immunohistochemistry, this is not the case for the second-line setting. Read More

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http://dx.doi.org/10.21037/jtd.2018.11.102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353746PMC
January 2019

The challenges of evaluating predictive biomarkers using small biopsy tissue samples and liquid biopsies from non-small cell lung cancer patients.

Authors:
Paul Hofman

J Thorac Dis 2019 Jan;11(Suppl 1):S57-S64

Université Côte d'Azur, CHU Nice, FHU OncoAge, Laboratory of Clinical and Experimental Pathology, Pasteur Hospital, Nice, France.

The list of theranostic biomarkers for the care of patients with advanced stage or metastatic non-small cell lung cancer has lengthened considerably these last few years. Moreover, the advances in therapeutics will certainly increase the number and complexity of these tests performed in laboratories in the near future. In addition, the methods for investigation of biomarkers that require access to biological tissue are less and less invasive with the consequential increase in the use of small-sized tissue biopsies and cytological and blood samples. Read More

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http://dx.doi.org/10.21037/jtd.2018.11.85DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353749PMC
January 2019

Circulating tumor cells (CTCs) for the noninvasive monitoring and personalization of non-small cell lung cancer (NSCLC) therapies.

J Thorac Dis 2019 Jan;11(Suppl 1):S45-S56

INSERM, U981 "Identification of Molecular Predictors and new Targets for Cancer Treatment", Villejuif, France.

Growing evidences for tumor heterogeneity confirm that single-tumor biopsies frequently fail to reveal the widespread mutagenic profile of tumor. Repeated biopsies are in most cases unfeasible, especially in advanced cancers. We describe here how circulating tumor cells (CTCs) isolated from minimally invasive blood sample might inform us about intratumor heterogeneity, tumor evolution and treatment resistance. Read More

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http://dx.doi.org/10.21037/jtd.2018.12.80DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353742PMC
January 2019

Advances in theranostic biomarkers for lung cancer from clinical to molecular pathology.

Authors:
Paul Hofman

J Thorac Dis 2019 Jan;11(Suppl 1):S1-S2

Université Côte d'Azur, CHU Nice, FHU OncoAge, Laboratory of Clinical and Experimental Pathology, Pasteur Hospital, Nice, France.

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http://dx.doi.org/10.21037/jtd.2018.10.28DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353740PMC
January 2019

Turning the corner on therapeutic cancer vaccines.

NPJ Vaccines 2019 8;4. Epub 2019 Feb 8.

2Vaccines Research and Development, Pfizer, Pearl River, NY 10965 USA.

Recent advances in several areas are rekindling interest and enabling progress in the development of therapeutic cancer vaccines. These advances have been made in target selection, vaccine technology, and methods for reversing the immunosuppressive mechanisms exploited by cancers. Studies testing different tumor antigens have revealed target properties that yield high tumor versus normal cell specificity and adequate immunogenicity to affect clinical efficacy. Read More

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http://www.nature.com/articles/s41541-019-0103-y
Publisher Site
http://dx.doi.org/10.1038/s41541-019-0103-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368616PMC
February 2019
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Nanotechnology-based approach for safer enrichment of semen with best spermatozoa.

J Anim Sci Biotechnol 2019 9;10:14. Epub 2019 Feb 9.

1Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS USA.

Background: Advances in nanotechnology have permitted molecular-based targeting of cells through safe and biocompatible magnetic nanoparticles (MNP). Their use to detect and remove damaged spermatozoa from semen doses could be of great interest. Here, MNP were synthesized and tested for their ability to target apoptotic (annexin V) and acrosome-reacted (lectin) boar spermatozoa, for high-throughout retrieval in a magnetic field (nanoselection). Read More

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http://dx.doi.org/10.1186/s40104-018-0307-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368687PMC
February 2019

Recent advances in proximity-based labeling methods for interactome mapping.

F1000Res 2019 31;8. Epub 2019 Jan 31.

Department of Cellular and Molecular Medicine and Ottawa Institute of Systems Biology, Faculty of Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada.

Proximity-based labeling has emerged as a powerful complementary approach to classic affinity purification of multiprotein complexes in the mapping of protein-protein interactions. Ongoing optimization of enzyme tags and delivery methods has improved both temporal and spatial resolution, and the technique has been successfully employed in numerous small-scale (single complex mapping) and large-scale (network mapping) initiatives. When paired with quantitative proteomic approaches, the ability of these assays to provide snapshots of stable and transient interactions over time greatly facilitates the mapping of dynamic interactomes. Read More

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http://dx.doi.org/10.12688/f1000research.16903.1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357996PMC
January 2019

Current concepts and recent advances in understanding and managing lumbar spine stenosis.

F1000Res 2019 31;8. Epub 2019 Jan 31.

Neurosurgery, University of Texas Southwestern Medical Center at Dallas, Dallas, TX, USA.

Lumbar spinal stenosis is a degenerative process that is extremely frequent in today's aging population. It can result in impingement on the nerves of the cauda equina or on the thecal sac itself, and lead to debilitating symptoms such as severe leg pain, or restriction in the perimeter of ambulation, both resulting in dependency in daily activities. The impact of the disease is global and includes financial repercussions because of its involvement in the active work force group. Read More

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http://dx.doi.org/10.12688/f1000research.16082.1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357993PMC
January 2019

Recent advances in clathrin-independent endocytosis.

F1000Res 2019 31;8. Epub 2019 Jan 31.

National Centre for Biological Sciences (NCBS-TIFR), Bangalore, Karnataka, 560065, India.

Endocytic pathways are broadly classified into clathrin dependent and independent on the basis of the requirement for the coat protein, clathrin. The molecular pathways and mechanisms underlying the formation of clathrin-independent pathways are still being explored, and this review summarizes recent advances and emerging functional roles of these diverse pathways. In particular, this review will discuss the growing consensus on the role of BAR domain proteins and the actin machinery in different clathrin-independent pathways and its significance to the functions fulfilled by these endocytic pathways. Read More

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http://dx.doi.org/10.12688/f1000research.16549.1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357988PMC
January 2019

Nucleotide and structural label identification in single RNA molecules with quantum tunneling spectroscopy.

Chem Sci 2019 Jan 5;10(4):1052-1063. Epub 2018 Nov 5.

Department of Chemical and Biological Engineering , University of Colorado Boulder , USA . Email:

Although a number of advances have been made in RNA sequencing and structural characterization, the lack of a method for directly determining the sequence and structure of single RNA molecules has limited our ability to probe heterogeneity in gene expression at the level of single cells. Here we present a method for direct nucleotide identification and structural label mapping of single RNA molecules Quantum Molecular Sequencing (QMSeq). The method combines non-perturbative quantum tunneling spectroscopy to probe the molecular orbitals of ribonucleotides, new experimental biophysical parameters that fingerprint these molecular orbitals, and a machine learning classification algorithm to distinguish between the ribonucleotides. Read More

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http://dx.doi.org/10.1039/c8sc03354dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6346406PMC
January 2019

Emerging approaches to study cell-cell interactions in tumor microenvironment.

Oncotarget 2019 Jan 22;10(7):785-797. Epub 2019 Jan 22.

Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.

Cell-cell interactions are of crucial importance for tissue formation, homeostasis, regeneration processes, and immune response. Recent studies underlined contribution of cell-cell interaction in tumor microenvironment (TME) for tumor progression and metastasis. Cancer cells modify the host cells to tumor-supportive traits, and the modified host cells contribute to tumor progression by interacting with cancer cells and further modifying other normal cells. Read More

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http://dx.doi.org/10.18632/oncotarget.26585DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366828PMC
January 2019

Wicked conflict: Using wicked problem thinking for holistic management of conservation conflict.

Conserv Lett 2018 Nov-Dec;11(6):e12460. Epub 2018 Apr 24.

Biological and Environmental Sciences, Faculty of Natural Sciences University of Stirling Stirling FK9 4LA United Kingdom.

Conservation conflict is widespread, damaging, and has proved difficult to manage using conventional conservation approaches. Conflicts are often "wicked problems," lacking clear solutions due to divergent values of stakeholders, and being embedded within wickedly complex environments. Drawing on the concept of wicked environmental problems could lead to management strategies better suited to tackling conflict. Read More

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http://dx.doi.org/10.1111/conl.12460DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360488PMC

Quest for cardiovascular interventions: precise modeling and 3D printing of heart valves.

J Biol Eng 2019 6;13:12. Epub 2019 Feb 6.

5Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana 124001 India.

Digitalization of health care practices is substantially manifesting itself as an effective tool to diagnose and rectify complex cardiovascular abnormalities. For cardiovascular abnormalities, precise non-invasive imaging interventions are being used to develop patient specific diagnosis and surgical planning. Concurrently, pre surgical 3D simulation and computational modeling are aiding in the effective surgery and understanding of valve biomechanics, respectively. Read More

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http://dx.doi.org/10.1186/s13036-018-0132-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366048PMC
February 2019

A spatio temporal spectral framework for plant stress phenotyping.

Plant Methods 2019 6;15:13. Epub 2019 Feb 6.

2Crop Science Group, Department of Environmental Systems Science, ETH Zürich, Zurich, Switzerland.

Background: Recent advances in high throughput phenotyping have made it possible to collect large datasets following plant growth and development over time, and those in machine learning have made inferring phenotypic plant traits from such datasets possible. However, there remains a dirth of datasets following plant growth under stress conditions along with methods for inferring them using only remotely sensed data, especially under a combination of multiple stress factors such as drought, weeds and nutrient deficiency. Such stress factors and their combinations are commonly encountered during crop production and being able to accurately detect and treat such stress conditions in an automated and timely manner can provide a major boost to farm yields with minimal resource input. Read More

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http://dx.doi.org/10.1186/s13007-019-0398-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364450PMC
February 2019

Application of Proteomics to Inflammatory Bowel Disease Research: Current Status and Future Perspectives.

Gastroenterol Res Pract 2019 15;2019:1426954. Epub 2019 Jan 15.

Gastroenterology Unit, Salford Royal Hospital, Salford, UK.

Inflammatory bowel disease (IBD) is a chronic relapsing/remitting inflammatory illness of the gastrointestinal tract of unknown aetiology. Despite recent advances in decoding the pathophysiology of IBD, many questions regarding disease pathogenesis remain. Genome-wide association studies (GWAS) and knockout mouse models have significantly advanced our understanding of genetic susceptibility loci and inflammatory pathways involved in IBD pathogenesis. Read More

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http://dx.doi.org/10.1155/2019/1426954DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350533PMC
January 2019

Metastatic tumor cells - genotypes and phenotypes.

Front Biol (Beijing) 2018 Aug 20;13(4):277-286. Epub 2018 Aug 20.

Department of Cardiothoracic Surgery, Department of Cell and Developmental Biology, Neuberger Berman Lung Cancer Center, Weill Cornell Medicine, New York, NY10065, USA.

Background: Metastasis is the primary cause of mortality in cancer patients. Therefore, elucidating the genetics and epigenetics of metastatic tumor cells and the mechanisms by which tumor cells acquire metastatic properties constitute significant challenges in cancer research.

Objective: To summarize the current understandings of the specific genotype and phenotype of the metastatic tumor cells. Read More

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http://dx.doi.org/10.1007/s11515-018-1513-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376987PMC

Systems Biology Approaches Toward Understanding Primary Mitochondrial Diseases.

Front Genet 2019 1;10:19. Epub 2019 Feb 1.

Mitochondrial Research Group, UCL Great Ormond Street Institute of Child Health, London, United Kingdom.

Primary mitochondrial diseases form one of the most common and severe groups of genetic disease, with a birth prevalence of at least 1 in 5000. These disorders are multi-genic and multi-phenotypic (even within the same gene defect) and span the entire age range from prenatal to late adult onset. Mitochondrial disease typically affects one or multiple high-energy demanding organs, and is frequently fatal in early life. Read More

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http://dx.doi.org/10.3389/fgene.2019.00019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6367241PMC
February 2019

Sociodemographic Differences in Humility: The Role of Social Relations.

Res Hum Dev 2018 26;15(1):50-71. Epub 2017 Dec 26.

University of Michigan,

The present research builds upon recent scientific developments in the field of social relations and ongoing advances in the study of humility. We examine whether gender, education, and racial/ethnic group are associated with humility as well as how social relations mediate the association between these sociodemographic variables and humility across the life course. Data are from the Detroit Community Study and were collected via a telephone survey focused on three groups prominent in the metro-Detroit area: Arab Americans, African Americans, and Non-Hispanic White Americans (N=907). Read More

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http://dx.doi.org/10.1080/15427609.2017.1414670DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6377239PMC
December 2017

Postoperative pain management practices and their effectiveness after major gynecological surgery: An observational study in a tertiary care hospital.

J Anaesthesiol Clin Pharmacol 2018 Oct-Dec;34(4):478-484

Department of Anaesthesiology, Aga Khan University, Karachi, Pakistan.

Background And Aims: Despite advances in postoperative pain management, patients continue to experience moderate to severe pain. This study was designed to assess the strategy, effectiveness, and safety of postoperative pain management in patients undergoing major gynecological surgery.

Material And Methods: This observational study included postoperative patients having major gynecological surgery from February 2016 to July 2016. Read More

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http://dx.doi.org/10.4103/joacp.JOACP_387_17DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360883PMC
February 2019

Documenting archaeological thin sections in high-resolution: A comparison of methods and discussion of applications.

Geoarchaeology 2019 Jan-Feb;34(1):100-114. Epub 2018 Nov 13.

ULS Digitisation and Document Delivery, KU Leuven Leuven Belgium.

Optical thin section observations represent the core empirical basis for most micromorphological interpretations at archaeological sites. These observations, which often vary in size and shape, are usually documented through digital graphic representations such as photomicrographs, scans, or figures. Due to variability in documentation practices, however, visual thin section data can be captured with a range of methods and in many different formats and resolutions. Read More

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http://dx.doi.org/10.1002/gea.21706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6358115PMC
November 2018

Anticipating governance challenges in synthetic biology: Insights from biosynthetic menthol.

Technol Forecast Soc Change 2019 Feb;139:311-320

Manchester Institute of Innovation Research, Alliance Manchester Business School, University of Manchester, Manchester M13 9PL, United Kingdom.

This paper advances an anticipatory governance framework to investigate and prepare for the potential implications of an emerging technology. Within the growing domain of synthetic biology, we draw on an end-to-end assessment of biosynthetic menthol that incorporates consideration of multiple dimensions of production and use. Based on documentary analysis, available data, and interviews, our approach unfolds in three steps. Read More

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http://dx.doi.org/10.1016/j.techfore.2018.11.020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360377PMC
February 2019

[Hidden pre-cancer clones in normal tissue].

Authors:
Bertrand Jordan

Med Sci (Paris) 2019 Feb 18;35(2):187-190. Epub 2019 Feb 18.

UMR 7268 ADÉS, Aix-Marseille, Université/EFS/CNRS ; CoReBio PACA, case 901, Parc scientifique de Luminy, 13288 Marseille Cedex 09, France.

Careful sequencing studies on small samples of normal oesophageal epithelium reveal the presence of very abundant cellular clones harbouring mutations in known cancer genes (and elsewhere). The number and size of these clones increases with age. This surprising finding confirms previous studies on sun-exposed epidermis. Read More

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http://dx.doi.org/10.1051/medsci/2019010DOI Listing
February 2019

[The eradication of infectious viral diseases endangered by advances in synthetic biology].

Med Sci (Paris) 2019 Feb 18;35(2):181-186. Epub 2019 Feb 18.

Institut de recherche biomédicale des armées, Unité Biothérapies anti-infectieuses et immunité, Département Microbiologie et maladies infectieuses, 1, place général Valérie André, 91220 Brétigny-sur-Orge, France - Institut Pasteur, unité génomique virale et vaccination, CNRS UMR-3569, 25, rue du Docteur Roux, 75015 Paris, France - École du Val-de-Grâce, 74, boulevard de Port-Royal, 75005 Paris, France.

The eradication of infectious diseases is one of the oldest dreams of mankind. It has been materialized only once in History with smallpox in 1980. Considerable efforts are being developed against poliomyelitis viruses since 1988, but the ultimate goal of eradication is not yet achieved. Read More

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http://dx.doi.org/10.1051/medsci/2019005DOI Listing
February 2019

Experimental models used in evaluating anti-tuberculosis vaccines: the latest advances in the field.

Expert Rev Vaccines 2019 Feb 16. Epub 2019 Feb 16.

b Basic Sciences Department , School of Medicine and Health Sciences, Universidad del Rosario , Carrera 24 No. 63C-69, Bogotá , Colombia.

Introduction: Tuberculosis is an infectious disease which is caused by bacilli from the M. tuberculosis complex. The Mycobacterium bovis Bacillus Calmette-Guérin vaccine is currently available as a prophylactic tool for preventing the disease; it has been shown to be efficient in preventing disseminated forms of tuberculosis during early ages, however, its efficiency is limited in areas where individuals have had prior exposure to environmental mycobacteria and its efficacy decreases with a host's age. Read More

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http://dx.doi.org/10.1080/14760584.2019.1583558DOI Listing
February 2019

Current Advances in Development of new Docetaxel Formulations.

Expert Opin Drug Deliv 2019 Feb 16. Epub 2019 Feb 16.

a CAS Key Laboratory of Experimental Marine Biology , Institute of Oceanology, Chinese Academy of Sciences , 266071 Qingdao , PR China.

Introduction: Docetaxel (DTX) is one of the most important chemotherapeutic agents and has been widely used for treatment of various types of cancers. However, the clinical chemotherapy of DTX gives many undesirable side effects due to the usage of organic solvent in the injection and its low selectivity for tumor cells. With the evolution of pharmaceutical technologies, great efforts have been paid to develop new DTX formulations to overcome these problems. Read More

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http://dx.doi.org/10.1080/17425247.2019.1583644DOI Listing
February 2019

Blood Cell Parameters and Predicting Coronary In-Stent Restenosis.

Angiology 2019 Feb 17:3319719830495. Epub 2019 Feb 17.

1 Department of Cardiology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.

Major advances in coronary interventional techniques and pharmacotherapy as well as the use of drug-eluting stents (DESs) have considerably reduced the risk of in-stent restenosis (ISR). However, ISR remains a major clinical challenge. Inflammation and platelet activation are important processes that underlie the pathophysiology of ISR. Read More

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http://dx.doi.org/10.1177/0003319719830495DOI Listing
February 2019

Energy-Converting Nanomedicine.

Small 2019 Feb 18:e1805339. Epub 2019 Feb 18.

State Key Laboratory of High Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, 200050, P. R. China.

Serious side effects to surrounding normal tissues and unsatisfactory therapeutic efficacy hamper the further clinic applications of conventional cancer-therapeutic strategies, such as chemotherapy and surgery. The fast development of nanotechnology provides unprecedented superiorities for cancer therapeutics. Externally activatable therapeutic modalities mediated by nanomaterials, relying on highly effective energy transformation to release therapeutic elements/effects (cytotoxic reactive oxygen species, thermal effect, photoelectric effect, Compton effect, cavitation effect, mechanical effect or chemotherapeutic drug) for cancer therapies, categorized and termed as "energy-converting nanomedicine," have arouse considerable concern due to their noninvasiveness, desirable tissue-penetration depth, and accurate modulation of therapeutic dose. Read More

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http://dx.doi.org/10.1002/smll.201805339DOI Listing
February 2019