2,307 results match your criteria Advances In Production Engineering & Management[Journal]


Moulding the mould: understanding and reprogramming filamentous fungal growth and morphogenesis for next generation cell factories.

Biotechnol Biofuels 2019 2;12:77. Epub 2019 Apr 2.

1Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308 China.

Filamentous fungi are harnessed as cell factories for the production of a diverse range of organic acids, proteins, and secondary metabolites. Growth and morphology have critical implications for product titres in both submerged and solid-state fermentations. Recent advances in systems-level understanding of the filamentous lifestyle and development of sophisticated synthetic biological tools for controlled manipulation of fungal genomes now allow rational strain development programs based on data-driven decision making. Read More

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http://dx.doi.org/10.1186/s13068-019-1400-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446404PMC

Ultrasonic Molding Technology: Recent Advances and Potential Applications in the Medical Industry.

Polymers (Basel) 2019 Apr 11;11(4). Epub 2019 Apr 11.

Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Av. Eugenio Garza Sada #2501 Sur, Monterrey NL 64849, Mexico.

Recently, ultrasonic molding (USM) has emerged as a promising replication technique for low and medium volume production of miniature and micro-scale parts. In a relatively short time cycle, ultrasonic molding can process a wide variety of polymeric materials without any noticeable thermal degradation into cost-effective molded parts. This research work reviews recent breakthroughs of the ultrasonic injection molding and ultrasonic compression molding process regarding the equipment and tooling development, materials processing and potential applications in the medical industry. Read More

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http://dx.doi.org/10.3390/polym11040667DOI Listing

Hypoxic Preconditioning Enhances Bone Marrow-Derived Mesenchymal Stem Cell Survival in a Low Oxygen and Nutrient-Limited 3D Microenvironment.

Cartilage 2019 Apr 11:1947603519841675. Epub 2019 Apr 11.

1 Department of Neurosurgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Objective: Skeletal tissues such as intervertebral disc and articular cartilage possess limited innate potential to regenerate, in part due to their avascularity and low cell density. Despite recent advances in mesenchymal stem cell (MSC)-based disc and cartilage regeneration, key challenges remain, including the sensitivity of these cells to in vivo microenvironmental stress such as low oxygen and limited nutrition. The objective of this study was to investigate whether preconditioning with hypoxia and/or transforming growth factor-β 3 (TGF-β3) can enhance MSC survival and extracellular matrix production in a low oxygen and nutrient-limited microenvironment. Read More

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http://dx.doi.org/10.1177/1947603519841675DOI Listing

Bioenergy and Biorefinery: feedstock, biotechnological conversion and products.

Biotechnol J 2019 Apr 10:e1800494. Epub 2019 Apr 10.

Department of Science, Technology and Innovation, Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe, Japan.

Biorefinery has been suggested to provide relevant substitutes to a number of fossil products. Feedstocks and conversion technologies have however, been the bottle-neck to the realization of this concept. Herein, feedstocks and bioconversion technologies under biorefinery have been reviewed. Read More

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http://dx.doi.org/10.1002/biot.201800494DOI Listing

Engineering Strategies in Microorganisms for the Enhanced Production of Squalene: Advances, Challenges and Opportunities.

Front Bioeng Biotechnol 2019 22;7:50. Epub 2019 Mar 22.

School of Biological Sciences and Biotechnology, Institute of Advanced Research, Koba Institutional Area, Gandhinagar, India.

The triterpene squalene is a natural compound that has demonstrated an extraordinary diversity of uses in pharmaceutical, nutraceutical, and personal care industries. Emboldened by this range of uses, novel applications that can gain profit from the benefits of squalene as an additive or supplement are expanding, resulting in its increasing demand. Ever since its discovery, the primary source has been the deep-sea shark liver, although recent declines in their populations and justified animal conservation and protection regulations have encouraged researchers to identify a novel route for squalene biosynthesis. Read More

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http://dx.doi.org/10.3389/fbioe.2019.00050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439483PMC

Systems biology based metabolic engineering for non-natural chemicals.

Biotechnol Adv 2019 Apr 4. Epub 2019 Apr 4.

Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada. Electronic address:

Production of chemicals in microorganisms is no longer restricted to products arising from native metabolic potential. In this review, we highlight the evolution of metabolic engineering studies, from the production of natural chemicals fermented from biomass hydrolysates, to the engineering of microorganisms for the production of non-natural chemicals. Advances in synthetic biology are accelerating the successful development of microbial cell factories to directly produce value-added chemicals. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2019.04.001DOI Listing
April 2019
1 Read

Advances in additive manufacturing for bone tissue engineering scaffolds.

Mater Sci Eng C Mater Biol Appl 2019 Jul 13;100:631-644. Epub 2019 Mar 13.

CONICET, National Scientific and Technical Research Council, Argentina; LAMEIN, Faculty of Exact Sciences and Engineering, National University of Tucuman, Argentina.

This article reviews the current state of the art of additive manufacturing techniques for the production of bone tissue engineering (BTE) scaffolds. The most well-known of these techniques include: stereolithography, selective laser sintering, fused deposition modelling and three-dimensional printing. This review analyses in detail the basic physical principles and main applications of these techniques and presents a list of biomaterials for BTE applications, including commercial trademarks. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09284931183079
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http://dx.doi.org/10.1016/j.msec.2019.03.037DOI Listing
July 2019
2 Reads

Metabolic engineering advances and prospects for amino acid production.

Metab Eng 2019 Mar 30. Epub 2019 Mar 30.

Genetics of Prokaryotes, Faculty of Biology and Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany. Electronic address:

Amino acid fermentation is one of the major pillars of industrial biotechnology. The multi-billion USD amino acid market is rising steadily and is diversifying. Metabolic engineering is no longer focused solely on strain development for the bulk amino acids L-glutamate and L-lysine that are produced at the million-ton scale, but targets specialty amino acids. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10967176193010
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http://dx.doi.org/10.1016/j.ymben.2019.03.008DOI Listing
March 2019
5 Reads
6.767 Impact Factor

Broadening the Scope of Biocatalysis in Sustainable Organic Synthesis.

ChemSusChem 2019 Apr 1. Epub 2019 Apr 1.

Molecular Sciences Institute, School of Chemistry, University of the Witwatersrand, Johannesburg, 2050, South Africa.

This Review is aimed at synthetic organic chemists who may be familiar with organometallic catalysis but have no experience with biocatalysis, and seeks to provide an answer to the perennial question: if it is so attractive, why wasn't it extensively used in the past? The development of biocatalysis in industrial organic synthesis is traced from the middle of the last century. Advances in molecular biology in the last two decades, in particular genome sequencing, gene synthesis and directed evolution of proteins, have enabled remarkable improvements in scope and substantially reduced biocatalyst development times and cost contributions. Additionally, improvements in biocatalyst recovery and reuse have been facilitated by developments in enzyme immobilization technologies. Read More

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http://dx.doi.org/10.1002/cssc.201900351DOI Listing
April 2019
3 Reads

Enzymatic and cell factory approaches to the production of human milk oligosaccharides.

Biotechnol Adv 2019 Mar 29. Epub 2019 Mar 29.

Laboratory of Biochemistry, Institut Químic de Sarrià, University Ramon Llull, 08017 Barcelona, Spain. Electronic address:

Infant formula milk companies try to develop fortified formula milk that mimics human milk as closely as possible, since it is well-known that breast milk has considerable implications in the development of the infant in the first years of life. Human milk is unique in terms of complex oligosaccharides content, known as human milk oligosaccharides (HMOs). Their role in the development of intestinal flora blocking the attachment of pathogens and modulating the immune system of the infant are currently recognized. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07349750193005
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http://dx.doi.org/10.1016/j.biotechadv.2019.03.014DOI Listing
March 2019
3 Reads

Precision Livestock Farming in Swine Welfare: A Review for Swine Practitioners.

Animals (Basel) 2019 Mar 31;9(4). Epub 2019 Mar 31.

Department of Electrical and Computer Engineering, College of Engineering, Michigan State University, 428 S Shaw Ln, East Lansing, MI 48824, USA.

The burgeoning research and applications of technological advances are launching the development of precision livestock farming. Through sensors (cameras, microphones and accelerometers), images, sounds and movements are combined with algorithms to non-invasively monitor animals to detect their welfare and predict productivity. In turn, this remote monitoring of livestock can provide quantitative and early alerts to situations of poor welfare requiring the stockperson's attention. Read More

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http://dx.doi.org/10.3390/ani9040133DOI Listing

Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens.

J Vis Exp 2019 Mar 14(145). Epub 2019 Mar 14.

Department of Genetics, Harvard Medical School; Wyss Institute for Biologically Inspired Engineering;

The marine bacterium Vibrio natriegens has garnered considerable attention as an emerging microbial host for biotechnology due to its fast growth rate. A general protocol is described for the preparation of V. natriegens crude cell extracts using common laboratory equipment. Read More

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https://www.jove.com/video/59495/cell-free-protein-expressio
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http://dx.doi.org/10.3791/59495DOI Listing
March 2019
3 Reads

Synthetic Biology Toolkits for Metabolic Engineering of Cyanobacteria.

Biotechnol J 2019 Mar 30:e1800496. Epub 2019 Mar 30.

Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 8 Medical Drive, Singapore, Singapore.

Cyanobacteria are of great importance to Earth's ecology. Due to their capability in photosynthesis and C1 metabolism, they are ideal microbial chassis that can be engineered for direct conversion of carbon dioxide and solar energy to biofuels and biochemicals. Facilitated by the elucidation of basic biology of the photoautotrophic microbes and rapid advances in synthetic biology, genetic toolkits have been developed to enable implementation of non-natural functionalities in engineered cyanobacteria. Read More

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http://dx.doi.org/10.1002/biot.201800496DOI Listing

Evolutionary engineering of Corynebacterium glutamicum.

Biotechnol J 2019 Mar 30:e1800444. Epub 2019 Mar 30.

Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich, Jülich, Germany.

A unique feature of biotechnology is that we can harness the power of evolution to improve process performance. Rational engineering of microbial strains has led to the establishment of a variety of successful bioprocesses, but is hampered by the overwhelming complexity of biological systems. Evolutionary engineering represents a straightforward approach for fitness-linked phenotypes (e. Read More

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http://dx.doi.org/10.1002/biot.201800444DOI Listing

Yeast Systems Biology: Model organism and Cell Factory.

Authors:
Jens Nielsen

Biotechnol J 2019 Mar 29:e1800421. Epub 2019 Mar 29.

BioInnovation Institute, Ole Måløes Vej 3, Copenhagen N, Denmark.

For thousands of years the yeast Saccharomyces cerevisiae has served as a cell factory for the production of bread, beer, and wine. In more recent years, this yeast has also served as a cell factory for producing many different fuels, chemicals, food ingredients and pharmaceuticals. S. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/biot.2018004
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http://dx.doi.org/10.1002/biot.201800421DOI Listing
March 2019
1 Read

Discovery of potential pathways for biological conversion of poplar wood into lipids by co-fermentation of strains.

Biotechnol Biofuels 2019 19;12:60. Epub 2019 Mar 19.

1Bioproducts, Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University, 2710 Crimson Way, Richland, WA 99354 USA.

Background: Biological routes for utilizing both carbohydrates and lignin are important to reach the ultimate goal of bioconversion of full carbon in biomass into biofuels and biochemicals. Recent biotechnology advances have shown promises toward facilitating biological transformation of lignin into lipids. Natural and engineered  strains (e. Read More

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http://dx.doi.org/10.1186/s13068-019-1395-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423811PMC

Electrospinning and Electrospun Nanofibers: Methods, Materials, and Applications.

Chem Rev 2019 Mar 27. Epub 2019 Mar 27.

The Wallace H. Coulter Department of Biomedical Engineering , Georgia Institute of Technology and Emory University , Atlanta , Georgia 30332 , United States.

Electrospinning is a versatile and viable technique for generating ultrathin fibers. Remarkable progress has been made with regard to the development of electrospinning methods and engineering of electrospun nanofibers to suit or enable various applications. We aim to provide a comprehensive overview of electrospinning, including the principle, methods, materials, and applications. Read More

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http://dx.doi.org/10.1021/acs.chemrev.8b00593DOI Listing

Novel therapeutic approaches in utilizing platelet lysate in regenerative medicine: Are we ready for clinical use?

J Cell Physiol 2019 Mar 25. Epub 2019 Mar 25.

Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Hemoderivative materials are used to treat different diseases. These derivatives include platelet-rich plasma, serum, platelet gel, and platelet lysate (PL). Among them, PL contains more growth factors than the others and its production is inexpensive and easy. Read More

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http://dx.doi.org/10.1002/jcp.28496DOI Listing
March 2019
2 Reads

Hydrogen peroxide generation from O electroreduction for environmental remediation: A state-of-the-art review.

Chemosphere 2019 Mar 12;225:588-607. Epub 2019 Mar 12.

Department of Civil and Environmental Engineering, Northeastern University, Boston, MA, 02115, USA. Electronic address:

The electrochemical production of hydrogen peroxide (HO) by 2-electron oxygen reduction reaction (ORR) is an attractive alternative to the present complex anthraquinone process. The objective of this paper is to provide a state-of-the-arts review of the most important aspects of this process. First, recent advances in HO production are reviewed and the advantages of HO electrogeneration via 2-electron ORR are highlighted. Read More

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http://dx.doi.org/10.1016/j.chemosphere.2019.03.042DOI Listing

Mitigating Clonal Variation in Recombinant Mammalian Cell Lines.

Trends Biotechnol 2019 Mar 18. Epub 2019 Mar 18.

The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark; Department of Biological Sciences, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. Electronic address:

Mammalian expression platforms are primary production systems for therapeutic proteins that require complex post-translational modifications. Current processes used for developing recombinant mammalian cell lines generate clonal cell lines with high phenotypic heterogeneity, which has puzzled researchers that use mammalian cell culture systems for a long time. Advances in mammalian genome-editing technologies and systems biotechnology have shed light on clonal variation and enabled rational cell engineering in a targeted manner. Read More

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http://dx.doi.org/10.1016/j.tibtech.2019.02.007DOI Listing
March 2019
2 Reads

4-Hydroxyproline-Derived Sustainable Polythioesters: Controlled Ring-Opening Polymerization, Complete Recyclability, and Facile Functionalization.

J Am Chem Soc 2019 Mar 20;141(12):4928-4935. Epub 2019 Mar 20.

Beijing National Laboratory for Molecular Sciences, Center for Soft Matter Science and Engineering, Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, College of Chemistry and Molecular Engineering , Peking University , Beijing 100871 , People's Republic of China.

The sustainable production of chemically recyclable polymers presents a significant opportunity to polymer scientists to tackle the growing environmental and energy problems of current petroleum-based plastics. Despite recent advances, however, there are still pressing needs for an expanded horizon of chemically recyclable polymers. Herein, we introduce a new paradigm of biosourced polythioesters (PTEs) with high polymerizability and complete recyclability under mild and economical conditions. Read More

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http://dx.doi.org/10.1021/jacs.9b00031DOI Listing
March 2019
12.113 Impact Factor

Visual Analytics of Genomic and Cancer Data: A Systematic Review.

Cancer Inform 2019 13;18:1176935119835546. Epub 2019 Mar 13.

The Tumour Bank, Children's Cancer Research Unit, Kids Research, The Children's Hospital at Westmead, Westmead, NSW, Australia.

Visual analytics and visualisation can leverage the human perceptual system to interpret and uncover hidden patterns in big data. The advent of next-generation sequencing technologies has allowed the rapid production of massive amounts of genomic data and created a corresponding need for new tools and methods for visualising and interpreting these data. Visualising genomic data requires not only simply plotting of data but should also offer a decision or a choice about what the message should be conveyed in the particular plot; which methodologies should be used to represent the results must provide an easy, clear, and accurate way to the clinicians, experts, or researchers to interact with the data. Read More

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http://journals.sagepub.com/doi/10.1177/1176935119835546
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http://dx.doi.org/10.1177/1176935119835546DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416684PMC
March 2019
16 Reads

Ferritin nanocage-based antigen delivery nanoplatforms: epitope engineering for peptide vaccine design.

Biomater Sci 2019 Mar 19. Epub 2019 Mar 19.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361102, China. and National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen University, Xiamen, 361102, China.

Biomedical applications and nanotechnological advances, including constrained synthesis, multimodal imaging, drug delivery, and bioassay, have strongly benefited from employing ferritin nanocages due to their remarkable properties of easy engineering, great biocompatible features, large capacity and so on. In this study, ferritin nanocages were used to display epitopes (model antigens derived from Enterovirus 71 (EV71) with different length) on C- and N-terminals and the loop zone to search for the optimal position for the fusion of the epitopes to the vaccine platform. The longest epitope displayed on the N-terminal and loop zone as well as the second longest peptide displayed on the loop zone of ferritin resulted in 100% passive protection of newborn BALB/c mice from the lethal EV71. Read More

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http://dx.doi.org/10.1039/c9bm00098dDOI Listing
March 2019
1 Read

Challenges and tackles in metabolic engineering for microbial production of carotenoids.

Microb Cell Fact 2019 Mar 18;18(1):55. Epub 2019 Mar 18.

Division of Applied Life Science (BK21 Plus), PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828, Republic of Korea.

Naturally occurring carotenoids have been isolated and used as colorants, antioxidants, nutrients, etc. in many fields. There is an ever-growing demand for carotenoids production. Read More

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http://dx.doi.org/10.1186/s12934-019-1105-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421696PMC

Large Area Substrate-Based Nanofabrication of Controllable and Customizable Gold Nanoparticles Via Capped Dewetting.

J Vis Exp 2019 Feb 26(144). Epub 2019 Feb 26.

Mechanical and Aerospace Engineering, Utah State University;

Recent scientific advances in the utilization of metallic nanoparticle for enhanced energy conversion efficiency, improved optical device performance, and high-density data storage have demonstrated the potential benefit of their use in industrial applications. These applications require precise control over nanoparticle size, spacing, and sometimes shape. These requirements have resulted in the use of time and cost intensive processing steps to produce nanoparticles, thus making the transition to industrial application unrealistic. Read More

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http://dx.doi.org/10.3791/58827DOI Listing
February 2019

Engineering Zymomonas mobilis for Robust Cellulosic Ethanol Production.

Trends Biotechnol 2019 Mar 13. Epub 2019 Mar 13.

State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address:

Great effort has been devoted to engineering Saccharomyces cerevisiae with pentose metabolism through the oxido-reductase pathway for cellulosic ethanol production, but intrinsic cofactor imbalance is observed, which substantially compromises ethanol yield. Zymomonas mobilis not only can be engineered for pentose metabolism through the isomerase pathway without cofactor imbalance but also metabolizes sugar through the Entner-Doudoroff pathway with less ATP and biomass produced for more sugar to be used for ethanol production. Moreover, the availabilities of genome sequence information for multiple Z. Read More

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http://dx.doi.org/10.1016/j.tibtech.2019.02.002DOI Listing
March 2019
1 Read

Oleaginous yeast for biofuel and oleochemical production.

Curr Opin Biotechnol 2019 Mar 12;57:73-81. Epub 2019 Mar 12.

Department of Chemical & Biomolecular Engineering, Clemson University, Clemson, SC 29634, United States. Electronic address:

Current transportation fuels derived from petroleum can also be made from microbial systems. In particular, oleaginous yeast have naturally evolved high flux pathways for fatty acids in the form of neutral lipids, which can be converted into a variety of drop-in fuels. Here, we describe the recent advances in the use of the four most popular oleaginous yeasts for making lipids and other potential fuels - Yarrowia lipolytica, Lipomyces starkeyi, Rhodosporidium toruloides, and Cutaneotrichosporon oleaginosus. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09581669183013
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http://dx.doi.org/10.1016/j.copbio.2019.02.011DOI Listing
March 2019
7 Reads

Advancing environmental exposure assessment science to benefit society.

Nat Commun 2019 03 15;10(1):1236. Epub 2019 Mar 15.

NYU School of Medicine, Department of Environmental Medicine, New York University, New York, NY, USA.

Awareness of the human health impacts of exposure to air pollution is growing rapidly. For example, it has become evident that the adverse health effects of air pollution are more pronounced in disadvantaged populations. Policymakers in many jurisdictions have responded to this evidence by enacting initiatives that lead to lower concentrations of air pollutants, such as urban traffic restrictions. Read More

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http://dx.doi.org/10.1038/s41467-019-09155-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420629PMC
March 2019
1 Read

New Applications of Synthetic Biology Tools for Cyanobacterial Metabolic Engineering.

Front Bioeng Biotechnol 2019 27;7:33. Epub 2019 Feb 27.

MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI, United States.

Cyanobacteria are promising microorganisms for sustainable biotechnologies, yet unlocking their potential requires radical re-engineering and application of cutting-edge synthetic biology techniques. In recent years, the available devices and strategies for modifying cyanobacteria have been increasing, including advances in the design of genetic promoters, ribosome binding sites, riboswitches, reporter proteins, modular vector systems, and markerless selection systems. Because of these new toolkits, cyanobacteria have been successfully engineered to express heterologous pathways for the production of a wide variety of valuable compounds. Read More

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http://dx.doi.org/10.3389/fbioe.2019.00033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400836PMC
February 2019
1 Read

Biomimetic hybrid membranes: incorporation of transport proteins/peptides into polymer supports.

Soft Matter 2019 Apr 14;15(13):2722-2736. Epub 2019 Mar 14.

Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, C/Eduard Maristany, 10-14, Ed. I2, 08019, Barcelona, Spain. and Barcelona Research Center for Multiscale Science and Engineering, Universitat Politècnica de Catalunya, C/Eduard Maristany, 10-14, Ed. C, 08019, Barcelona, Spain.

Molecular sensing, water purification and desalination, drug delivery, and DNA sequencing are some striking applications of biomimetic hybrid membranes. These devices take advantage of biomolecules, which have gained excellence in their specificity and efficiency during billions of years, and of artificial materials that load the purified biological molecules and provide technological properties, such as robustness, scalability, and suitable nanofeatures to confine the biomolecules. Recent methodological advances allow more precise control of polymer membranes that support the biomacromolecules, and are expected to improve the design of the next generation of membranes as well as their applicability. Read More

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http://dx.doi.org/10.1039/c8sm02513dDOI Listing

Designing Resorbable Scaffolds for Bone Defects.

Bull Hosp Jt Dis (2013) 2019 Mar;77(1):39-44

The next frontier of orthopedic implants are resorbable devices. Tissue engineering advances have created a demand for scaffolds that can facilitate biologic regeneration. Scaffolds that will degrade over time with the infiltration of host cells are of particular interest. Read More

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Emerging biomaterials for downstream manufacturing of therapeutic proteins.

Acta Biomater 2019 Mar 9. Epub 2019 Mar 9.

Department of Chemical and Biomolecular Engineering, and Institute for NanoBioTechnology, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, United States; Department of Oncology and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21205, United States. Electronic address:

Downstream processing is considered one of the most challenging phases of industrial manufacturing of therapeutic proteins, accounting for a large portion of the total production costs. The growing demand for therapeutic proteins in the biopharmaceutical market in addition to a significant rise in upstream titers have placed an increasing burden on the downstream purification process, which is often limited by high cost and insufficient capacities. To achieve efficient production and reduced costs, a variety of biomaterials have been exploited to improve the current techniques and also to develop superior alternatives. Read More

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http://dx.doi.org/10.1016/j.actbio.2019.03.015DOI Listing
March 2019
1 Read
6.025 Impact Factor

National and Transnational Security Implications of Asymmetric Access to and Use of Biological Data.

Front Bioeng Biotechnol 2019 25;7:21. Epub 2019 Feb 25.

Promontory Financial Group, an IBM Company, Washington, DC, United States.

Biology and biotechnology have changed dramatically during the past 20 years, in part because of increases in computational capabilities and use of engineering principles to study biology. The advances in supercomputing, data storage capacity, and cloud platforms enable scientists throughout the world to generate, analyze, share, and store vast amounts of data, some of which are biological and much of which may be used to understand the human condition, agricultural systems, evolution, and environmental ecosystems. These advances and applications have enabled: (1) the emergence of data science, which involves the development of new algorithms to analyze and visualize data; and (2) the use of engineering approaches to manipulate or create new biological organisms that have specific functions, such as production of industrial chemical precursors and development of environmental bio-based sensors. Read More

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http://dx.doi.org/10.3389/fbioe.2019.00021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397826PMC
February 2019

Recent Advances in Electrosynthesized Molecularly Imprinted Polymer Sensing Platforms for Bioanalyte Detection.

Sensors (Basel) 2019 Mar 9;19(5). Epub 2019 Mar 9.

Faculty of Science & Engineering, Div. of Chemistry & Environmental Science, Manchester Metropolitan University, John Dalton Building, Chester Street, Manchester M1 5GD, UK.

The accurate detection of biological materials has remained at the forefront of scientific research for decades. This includes the detection of molecules, proteins, and bacteria. Biomimetic sensors look to replicate the sensitive and selective mechanisms that are found in biological systems and incorporate these properties into functional sensing platforms. Read More

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https://www.mdpi.com/1424-8220/19/5/1204
Publisher Site
http://dx.doi.org/10.3390/s19051204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6427210PMC
March 2019
3 Reads

Advances in Selective Laser Melting of Nitinol Shape Memory Alloy Part Production.

Materials (Basel) 2019 Mar 8;12(5). Epub 2019 Mar 8.

School of Mechanical & Manufacturing Engineering, Dublin City University, Dublin 9, Ireland.

Nitinol (nickel-titanium or Ni-Ti) is the most utilized shape memory alloy due to its good superelasticity, shape memory effect, low stiffness, damping, biocompatibility, and corrosion resistance. Various material characteristics, such as sensitivity to composition and production thermal gradients, make conventional methods ineffective for the manufacture of high quality complex Nitinol components. These issues can be resolved by modern additive manufacturing (AM) methods which can produce net or near-net shape parts with highly precise and complex Nitinol structures. Read More

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http://dx.doi.org/10.3390/ma12050809DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6427257PMC
March 2019
1 Read

Synthetic biology and metabolic engineering of actinomycetes for natural product discovery.

Biotechnol Adv 2019 Mar 7. Epub 2019 Mar 7.

Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, 2800 Kgs. Lyngby, Denmark. Electronic address:

Actinomycetes are one of the most valuable sources of natural products with industrial and medicinal importance. After more than half a century of exploitation, it has become increasingly challenging to find novel natural products with useful properties as the same known compounds are often repeatedly re-discovered when using traditional approaches. Modern genome mining approaches have led to the discovery of new biosynthetic gene clusters, thus indicating that actinomycetes still harbor a huge unexploited potential to produce novel natural products. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2019.03.005DOI Listing

Bioengineered bacterial vesicles as biological nano-heaters for optoacoustic imaging.

Nat Commun 2019 03 7;10(1):1114. Epub 2019 Mar 7.

Chair of Biological Imaging, TranslaTUM, Technische Universität München, Munich, 81675, Germany.

Advances in genetic engineering have enabled the use of bacterial outer membrane vesicles (OMVs) to deliver vaccines, drugs and immunotherapy agents, as a strategy to circumvent biocompatibility and large-scale production issues associated with synthetic nanomaterials. We investigate bioengineered OMVs for contrast enhancement in optoacoustic (photoacoustic) imaging. We produce OMVs encapsulating biopolymer-melanin (OMV) using a bacterial strain expressing a tyrosinase transgene. Read More

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http://dx.doi.org/10.1038/s41467-019-09034-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405847PMC
March 2019
2 Reads

Advances in the metabolic engineering of Yarrowia lipolytica for the production of terpenoids.

Bioresour Technol 2019 Jun 27;281:449-456. Epub 2019 Feb 27.

College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China; Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, No. 5 Xinmofan Road, Nanjing 210009, People's Republic of China. Electronic address:

Terpenoids are a large class of natural compounds based on the C5 isoprene unit, with many biological effects such activity against cancer and allergies, while some also have an agreeable aroma. Consequently, they have received extensive attention in the food, pharmaceutical and cosmetic fields. With the identification and analysis of the underlying natural product synthesis pathways, current microbial-based metabolic engineering approaches have yielded new strategies for the production of highly valuable terpenoids. Read More

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http://dx.doi.org/10.1016/j.biortech.2019.02.116DOI Listing
June 2019
2 Reads

A Pivotal Role of Hormones in Regulating Cotton Fiber Development.

Front Plant Sci 2019 14;10:87. Epub 2019 Feb 14.

Key Laboratory of the Ministry of Education for Medicinal Plant Resources and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi'an, China.

Cotton is the main source of renewable fiber in the world and is primarily used for textile production. Cotton fibers are single cells differentiated from the ovule epidermis and are an excellent model system for studying cell elongation, polyploidization, and cell wall biosynthesis. Plant hormones, which are present in relatively low concentrations, play important roles in various developmental processes, and recently, multiple reports have revealed the pivotal roles of hormones in regulating cotton fiber development. Read More

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http://dx.doi.org/10.3389/fpls.2019.00087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6382683PMC
February 2019

CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture.

Annu Rev Plant Biol 2019 Mar 5. Epub 2019 Mar 5.

State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China 100101; email:

Enhanced agricultural production through innovative breeding technology is urgently needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas genome editing enable efficient targeted modification in most crops, thus promising to accelerate crop improvement. Here, we review advances in CRISPR/Cas9 and its variants and examine their applications in plant genome editing and related manipulations. Read More

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https://www.annualreviews.org/doi/10.1146/annurev-arplant-05
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http://dx.doi.org/10.1146/annurev-arplant-050718-100049DOI Listing
March 2019
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A bifunctional UDP-sugar 4-epimerase supports biosynthesis of multiple cell surface polysaccharides in .

J Bacteriol 2019 Mar 4. Epub 2019 Mar 4.

LOEWE Center for Synthetic Microbiology (SYNMIKRO), Philipps University Marburg, Marburg, Germany

produces multiple extracellular glycans, including among others lipopolysaccharides (LPS), and the exopolysaccharides (EPS) succinoglycan (SG) and galactoglucan (GG). These polysaccharides serve cell protective roles. Furthermore, SG and GG promote interaction of with its host in root nodule symbiosis. Read More

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http://dx.doi.org/10.1128/JB.00801-18DOI Listing

On the use of oxygenic photosynthesis for the sustainable production of commodity chemicals.

Physiol Plant 2019 Mar 4. Epub 2019 Mar 4.

Molecular Microbial Physiology Group, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH Amsterdam, The Netherlands.

A sustainable society will have to largely refrain from the use of fossil carbon deposits. In such a regime, renewable electricity can be harvested as a primary source of energy. However, as for the synthesis of carbon-based materials from bulk chemicals, an alternative is required. Read More

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http://dx.doi.org/10.1111/ppl.12946DOI Listing
March 2019
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The fickle CHO: a review of the causes, implications, and potential alleviation of the CHO cell line instability problem.

Curr Opin Biotechnol 2019 Feb 28;60:128-137. Epub 2019 Feb 28.

Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, United States; Dept. of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716, United States. Electronic address:

Chinese hamster ovary (CHO) cell-based bioproduction of recombinant proteins can now routinely achieve >5 g/L titers in fed-batches. This progress is partly due to the rapid adaptability of CHO cells to various genetic manipulations and changing process conditions. An inherently plastic genome allows for this adaptability; however, it also gives CHO cells the propensity for genomic rearrangements. Read More

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http://dx.doi.org/10.1016/j.copbio.2019.01.011DOI Listing
February 2019
7.117 Impact Factor

Multiplex Genome Engineering for Optimizing Bioproduction in Saccharomyces cerevisiae.

Biochemistry 2019 Mar 8;58(11):1492-1500. Epub 2019 Mar 8.

Manchester Institute of Biotechnology (MIB), School of Chemistry , The University of Manchester , 131 Princess Street , Manchester M1 7DN , United Kingdom.

The field of synthetic biology is already beginning to realize its potential, with a wealth of examples showcasing the successful genetic engineering of microorganisms for the production of valuable compounds. The chassis Saccharomyces cerevisiae has been engineered to function as a microfactory for producing many of these economically and medically relevant compounds. However, strain construction and optimization to produce industrially relevant titers necessitate a wealth of underpinning biological knowledge alongside large investments of capital and time. Read More

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http://dx.doi.org/10.1021/acs.biochem.8b01086DOI Listing
March 2019
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Recent advances in lignin valorization with bacterial cultures: microorganisms, metabolic pathways, and bio-products.

Biotechnol Biofuels 2019 15;12:32. Epub 2019 Feb 15.

1School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094 China.

Lignin is the most abundant aromatic substrate on Earth and its valorization technologies are still under developed. Depolymerization and fragmentation are the predominant preparatory strategies for valorization of lignin to chemicals and fuels. However, due to the structural heterogeneity of lignin, depolymerization and fragmentation typically result in diverse product species, which require extensive separation and purification procedures to obtain target products. Read More

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http://dx.doi.org/10.1186/s13068-019-1376-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376720PMC
February 2019

Advances in antibody engineering for rheumatic diseases.

Nat Rev Rheumatol 2019 Apr;15(4):197-207

Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology (ETH Zürich), Zürich, Switzerland.

The advent of biologic therapies, particularly antibody therapeutics, has revolutionized the pharmacological treatment of many rheumatic diseases. Antibody discovery began with the immunization of mice for the production of rodent immunoglobulins, but advances in protein and genetic engineering have now made it possible to generate fully human antibodies, which are better tolerated by patients. For most clinical applications in rheumatology, antibodies have been used as blocking agents capable of neutralizing the function of pro-inflammatory proteins, such as TNF. Read More

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http://www.nature.com/articles/s41584-019-0188-8
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http://dx.doi.org/10.1038/s41584-019-0188-8DOI Listing
April 2019
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MultiBac: Baculovirus-Mediated Multigene DNA Cargo Delivery in Insect and Mammalian Cells.

Viruses 2019 Feb 26;11(3). Epub 2019 Feb 26.

School of Biochemistry, Biomedical Sciences, University of Bristol, 1 Tankard's Close, Bristol BS8 1TD, UK.

The baculovirus/insect cell system (BICS) is widely used in academia and industry to produce eukaryotic proteins for many applications, ranging from structure analysis to drug screening and the provision of protein biologics and therapeutics. Multi-protein complexes have emerged as vital catalysts of cellular function. In order to unlock the structure and mechanism of these essential molecular machines and decipher their function, we developed MultiBac, a BICS particularly tailored for heterologous multigene transfer and multi-protein complex production. Read More

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http://dx.doi.org/10.3390/v11030198DOI Listing
February 2019
3 Reads

Advances in Capacitive Micromachined Ultrasonic Transducers.

Micromachines (Basel) 2019 Feb 23;10(2). Epub 2019 Feb 23.

E.L. Ginzton Lab., Stanford University, Stanford, CA 94305, USA.

Capacitive micromachined ultrasonic transducer (CMUT) technology has enjoyed rapid development in the last decade. Advancements both in fabrication and integration, coupled with improved modelling, has enabled CMUTs to make their way into mainstream ultrasound imaging systems and find commercial success. In this review paper, we touch upon recent advancements in CMUT technology at all levels of abstraction; modeling, fabrication, integration, and applications. Read More

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http://dx.doi.org/10.3390/mi10020152DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412242PMC
February 2019

Linkages between stratospheric ozone, UV radiation and climate change and their implications for terrestrial ecosystems.

Photochem Photobiol Sci 2019 Mar 27;18(3):681-716. Epub 2019 Feb 27.

Department of Forest, Rangeland and Fire Sciences, University of Idaho, Moscow, ID, USA.

Exposure of plants and animals to ultraviolet-B radiation (UV-B; 280-315 nm) is modified by stratospheric ozone dynamics and climate change. Even though stabilisation and projected recovery of stratospheric ozone is expected to curtail future increases in UV-B radiation at the Earth's surface, on-going changes in climate are increasingly exposing plants and animals to novel combinations of UV-B radiation and other climate change factors (e.g. Read More

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http://dx.doi.org/10.1039/c8pp90061bDOI Listing
March 2019
1 Read