Trends Biotechnol 2022 May 17. Epub 2022 May 17.
Biological Science Research Center, Southwest University, Chongqing 400716, China; Wound Trauma Medical Center, State Key Laboratory of Trauma, Burns, and Combined Injury, Daping Hospital, Army Medical University, Chongqing 400042, China; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. Electronic address:
An ideal molecular diagnostic method should be sensitive, specific, low cost, rapid, portable, and easy to operate. Traditional nucleic acid detection methods based mainly on PCR technology have not only high sensitivity and specificity, but also some limitations, such as the need for expensive equipment and skilled technicians, being both time and labor intensive, and difficult to implement in some regions. However, with the continuous development of CRISPR-Cas technology and its application in molecular diagnosis, new approaches have been used for the construction of molecular diagnostic systems. Read More