385 results match your criteria Adipocyte[Journal]


Novel semi-automated algorithm for high-throughput quantification of adipocyte size in breast adipose tissue, with applications for breast cancer microenvironment.

Adipocyte 2020 Dec;9(1):313-325

BU-BMC Cancer Center, Boston University School of Medicine , Boston, MA, USA.

The size distribution of adipocytes in fat tissue provides important information about metabolic status and overall health of patients. Histological measurements of biopsied adipose tissue can reveal cardiovascular and/or cancer risks, to complement typical prognosis parameters such as body mass index, hypertension or diabetes. Yet, current methods for adipocyte quantification are problematic and insufficient. Read More

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http://dx.doi.org/10.1080/21623945.2020.1787582DOI Listing
December 2020

Identification of differentially expressed mRNA and the Hub mRNAs modulated by lncRNA as a competing endogenous RNA in brown adipose tissue of mice on a high-fat diet.

Adipocyte 2020 Dec;9(1):346-358

Department of Pathology & Pathophysiology, Wuhan University School of Basic Medical Sciences , Wuhan, China.

Obesity is associated with insulin resistance, diabetes, and obesity-related metabolic disorders. Brown adipocytes have emerged as potential targets for the treatment of obesity and obesity-related diseases. However, changes that occur in brown adipose tissue during various stages of high fat diet (HFD)-induced obesity remain poorly understood. Read More

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http://dx.doi.org/10.1080/21623945.2020.1789283DOI Listing
December 2020

Inhibition of γ-secretase in adipocytes leads to altered IL-6 secretion and adipose inflammation.

Adipocyte 2020 Dec;9(1):325-334

Department of Medicine, University of Oklahoma Health Sciences Center , Oklahoma City, OK, USA.

Adipocyte-mediated inflammatory signalling has been proposed to alter adipose physiology in obesity and Type 2 diabetes mellitus. Novel targets for alteration of inflammatory signalling are needed to improve obesity-related outcomes. The γ-secretase enzyme complex has been suggested to play a role both in adipocyte function as well as in immune regulation. Read More

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http://dx.doi.org/10.1080/21623945.2020.1788235DOI Listing
December 2020

Ginsenoside Rb2 improves insulin resistance by inhibiting adipocyte pyroptosis.

Adipocyte 2020 Dec;9(1):302-312

Department of Endocrine and Metabolic Diseases, 1st Affiliated Hospital of Wenzhou Medical University , Wenzhou City, Zhejiang Province, P.R. China.

Pyroptosis plays a critical role in the development of obesity-associated inflammation and insulin resistantance (IR). Ginsenoside Rb2 (Rb2), the main component of ginsenosides has drawn appreciable interest in the context of glucose metabolism. In the present study, we investigated Rb2-mediated protection against obesity-induced IR and the related mechanisms. Read More

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http://dx.doi.org/10.1080/21623945.2020.1778826DOI Listing
December 2020

A new human adipocyte model with PTEN haploinsufficiency.

Adipocyte 2020 Dec;9(1):290-301

Hospital for Children & Adolescents, Center for Pediatric Research, Leipzig University , Leipzig, Germany.

Few human cell strains are suitable and readily available as adipocyte models. We used resected lipoma tissue from a patient with germline phosphatase and tensin homolog (PTEN) haploinsufficiency to establish a preadipocyte cell strain termed LipPD1 and aimed to characterize cellular functions and signalling pathway alterations in comparison to the established adipocyte model Simpson-Golabi-Behmel-Syndrome (SGBS) and to primary stromal-vascular fraction cells. We found that both cellular life span and the capacity for adipocyte differentiation as well as adipocyte-specific functions were preserved in LipPD1 and comparable to SGBS adipocytes. Read More

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http://dx.doi.org/10.1080/21623945.2020.1785083DOI Listing
December 2020

The effect of Acot2 overexpression or downregulation on the preadipocyte differentiation in Chinese Red Steppe cattle.

Adipocyte 2020 Dec;9(1):279-289

Branch of Animal Husbandry, Jilin Academy of Agricultural Science , Gongzhuling, China.

The quality and nutritional value of beef is closely linked to its content of intramuscular fat (IMF). The differentiation of preadipocytes and the deposition of lipid droplets in the adipocytes are the key to regulate the IMF content. The differentiation of adipocytes is regulated by a series of transcription factors and genes. Read More

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http://dx.doi.org/10.1080/21623945.2020.1776553DOI Listing
December 2020

TSH promotes adiposity by inhibiting the browning of white fat.

Adipocyte 2020 Dec;9(1):264-278

Department of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong First Medical University , Shandong, P.R. China.

Adiposity is caused by an imbalance between energy intake and consumption. Promotion of the browning of white fat increases energy expenditure and could combat adiposity. Thyroid-stimulating hormone (TSH) has been confirmed to positively correlate with adiposity. Read More

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http://dx.doi.org/10.1080/21623945.2020.1783101DOI Listing
December 2020

The common pathophysiologic threads between Asian Indian diabetic's 'Thin Fat Phenotype' and partial lipodystrophy: the peripheral adipose tissue transcriptomic evidences.

Adipocyte 2020 Jan;9(1):253-263

Department of Endocrinology, Sawai Man Singh Medical College and Hospital , Jaipur, India.

T2D is a complex disease with poorly understood mechanisms. In Asian Indians, it is associated with "thin fat" phenotype which resembles with partial lipodystrophy. We hypothesized that disturbed expression of lipodystrophy genes might play a role in T2D pathogenesis. Read More

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http://dx.doi.org/10.1080/21623945.2020.1776082DOI Listing
January 2020

Cathepsin gene expression in abdominal subcutaneous adipose tissue of obese/overweight humans.

Adipocyte 2020 Jan;9(1):246-252

Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+ , Maastricht, The Netherlands.

Cathepsin L1 (CTSL1) and B (CTSB) are lysosomal proteases, of which the expression and activity are impaired in adipose tissue (AT) of obese rodents, indicating AT lysosomal dysfunction. Here we assess the relation between abdominal subcutaneous AT (SCAT) CTSL1 and CTSB expression (qRT-PCR), body composition and tissue-specific insulin resistance in 77 overweight/obese (BMI: 225.6-38. Read More

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http://dx.doi.org/10.1080/21623945.2020.1775035DOI Listing
January 2020

Elastin-derived peptide VGVAPG decreases differentiation of mouse embryo fibroblast (3T3-L1) cells into adipocytes.

Adipocyte 2020 Dec;9(1):234-245

Department of Lifestyle Disorders and Regenerative Medicine, University of Information Technology and Management in Rzeszow , Rzeszow, Poland.

Elastin is a highly elastic protein present in connective tissue. As a result of protease activity, elastin hydrolysis occurs, and during this process, elastin-derived peptides (EDPs) are released. One of the constitutively repeating elastin and EDP building sequences is the hexapeptide VGVAPG. Read More

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http://dx.doi.org/10.1080/21623945.2020.1770525DOI Listing
December 2020

Role of TLR4 in the induction of inflammatory changes in adipocytes and macrophages.

Adipocyte 2020 Dec;9(1):212-222

Department of Pediatrics and Communicable Disease, University of Michigan Medical School, Ann Arbor, MI, USA.

In obesity, high levels of saturated fatty acids (SFAs) contribute to adipose tissue inflammation and dysfunction. Obesity-induced macrophage infiltration leads to insulin resistance, but the adipocyte itself may play a role in generating the inflammatory milieu. Given our recent findings of the role of TLR4 in myeloid biasing in obesity, we next investigated the role of TLR4 in adipocyte generated inflammatory responses to SFAs and lipopolysaccharides. Read More

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http://dx.doi.org/10.1080/21623945.2020.1760674DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238871PMC
December 2020

Serum leptin levels are positively associated with aortic stiffness in patients with chronic kidney disease stage 3-5.

Adipocyte 2020 Dec;9(1):206-211

School of Medicine, Tzu Chi University, Hualien, Taiwan.

Leptin potentially exerts atherogenic effects.This study evaluated the relationship between serum leptin levels and aortic stiffness in patients with stage 3-5 chronic kidney disease (CKD). Totally 205 participants were enrolled. Read More

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http://dx.doi.org/10.1080/21623945.2020.1764799DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238868PMC
December 2020

Long term hypoxia during gestation alters perirenal adipose tissue gene expression in the lamb.

Adipocyte 2020 Dec;9(1):223-233

Lawrence D. Longo M.D. Center for Perinatal Biology, School of Medicine, Loma Linda University, Loma Linda, CA, USA.

We previously reported that following long-term hypoxia (LTH), the ovine foetus exhibits enhanced expression of brown/beige adipose genes. This study was designed to determine if these changes are preserved after birth. Pregnant ewes were divided among three groups, 1) Control, sea level, 2) LTH, high altitude (3,820 m, LTH-HA) from ~ day 40 of gestation through ~14 days post-delivery and 3) LTH from ⁓ day 40 through day 137 of gestation then returned to the laboratory where atory reduced maternal PO was maintained by nitrogen infusion. Read More

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http://dx.doi.org/10.1080/21623945.2020.1763726DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238872PMC
December 2020

Towards precision medicine: defining and characterizing adipose tissue dysfunction to identify early immunometabolic risk in symptom-free adults from the GEMM family study.

Adipocyte 2020 12;9(1):153-169

Population Health Program, Texas Biomedical Research Institute and Southwest National Primate Research Center (SNPRC), San Antonio, TX, USA.

Interactions between macrophages and adipocytes are early molecular factors influencing adipose tissue (AT) dysfunction, resulting in high leptin, low adiponectin circulating levels and low-grade metaflammation, leading to insulin resistance (IR) with increased cardiovascular risk. We report the characterization of AT dysfunction through measurements of the adiponectin/leptin ratio (ALR), the adipo-insulin resistance index (Adipo-IRi), fasting/postprandial (F/P) immunometabolic phenotyping and direct F/P differential gene expression in AT biopsies obtained from symptom-free adults from the GEMM family study. AT dysfunction was evaluated through associations of the ALR with F/P insulin-glucose axis, lipid-lipoprotein metabolism, and inflammatory markers. Read More

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http://dx.doi.org/10.1080/21623945.2020.1743116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153654PMC
December 2020

MiR-130a in the adipogenesis of human SGBS preadipocytes and its susceptibility to androgen regulation.

Adipocyte 2020 12;9(1):197-205

Center for Reproductive Medicine and Andrology, University Hospital Halle (Saale), Martin Luther University Halle-Wittenberg, Halle, Germany.

: Adipogenesis is the differentiation process generating mature adipocytes from undifferentiated mesenchymal stem cells. The differentiation can be inhibited by androgens, although knowledge about intracellular effectors of this inhibition is scarce. Recently, androgen-regulated microRNAs were detected as interesting candidates in this context. Read More

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http://dx.doi.org/10.1080/21623945.2020.1750256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153545PMC
December 2020

MiR-455 targeting SOCS3 improve liver lipid disorders in diabetic mice.

Adipocyte 2020 12;9(1):179-188

Department of Endocrinology & Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.

MiR-455 has been verified a key regulator of brown adipose tissue and adipose tissue-specific overexpression of miR-455 (ap2-miR-455) mice could combat high-fat-diet-induced obesity. This study is to verify overexpression of miR-455 could ameliorate the lipid accumulation and metabolism in the liver of db/db diabetic mice and explore the potential mechanisms. Diabetic mice (db/db) and control mice (db/m) were randomly divided into four groups. Read More

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http://dx.doi.org/10.1080/21623945.2020.1749495DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153657PMC
December 2020

Transplantation of adipose tissue lacking PAI-1 improves glucose tolerance and attenuates cardiac metabolic abnormalities in high-fat diet-induced obesity.

Adipocyte 2020 12;9(1):170-178

Key Laboratory of Medical Electrophysiology of Ministry of Education, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease of Sichuan Province, Drug Discovery Research Center, Southwest Medical University, Luzhou, China.

Adipose tissue is an important metabolic organ, and transplantation of white adipose tissue plays crucial roles in glucose homoeostasis and energy metabolism. However, how adipose tissue affects glucose utilization is poorly understood. PAI-1-knockout (PAI-1KO) mice were previously shown to be resistant to a high-fat diet and obesity. Read More

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http://dx.doi.org/10.1080/21623945.2020.1748961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153656PMC
December 2020

Depot-specific adipocyte-extracellular matrix metabolic crosstalk in murine obesity.

Adipocyte 2020 12;9(1):189-196

Department of Surgery, University of Michigan Medical School, Ann Arbor, MI, USA.

Subcutaneous (SAT) and visceral (VAT) adipose tissues have distinct metabolic phenotypes. We hypothesized that the extracellular matrix (ECM) regulates depot-specific differences in adipocyte metabolic function in murine obesity. VAT and SAT preadipocytes from lean or obese mice were subject to adipogenic differentiation in standard 2D culture on plastic tissue culture plates or in 3D culture in ECM, followed by metabolic profiling. Read More

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http://dx.doi.org/10.1080/21623945.2020.1749500DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153651PMC
December 2020

Insulin-induced serine 22 phosphorylation of retinoid X receptor alpha is dispensable for adipogenesis in brown adipocytes.

Adipocyte 2020 12;9(1):142-152

Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

Insulin action initiates a series of phosphorylation events regulating cellular differentiation, growth and metabolism. We have previously discovered, in a mass spectrometry-based phosphoproteomic study, that insulin/IGF-1 signalling induces phosphorylation of retinoid x receptor alpha (RXRα) at S22 in mouse brown pre-adipocytes. Here, we show that insulin induces the phosphorylation of RXRα at S22 in both brown precursor and mature adipocytes through a pathway involving ERK, downstream of IRS-1 and -2. Read More

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http://dx.doi.org/10.1080/21623945.2020.1747352DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153655PMC
December 2020

Involvement of calcium channels in the regulation of adipogenesis.

Adipocyte 2020 12;9(1):132-141

Huazhong University of Science and Technology Union Shenzhen Hospital and the 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China.

As an important second messenger in adipocytes, calcium ions (Ca) are essential in regulating various intracellular signalling pathways that control critical cellular functions. Calcium channels show selective permeability to Ca and facilitate Ca entry into the cytoplasm, which are normally located in the plasmatic and intracellular membranes. The increase of cytosolic Ca modulates a variety of signalling pathways and results in the transcription of target genes that contribute to adipogenesis, a key cellular event includes proliferation and differentiation of adipocyte. Read More

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http://dx.doi.org/10.1080/21623945.2020.1738792DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153653PMC
December 2020

Low and high concentrations of butyrate regulate fat accumulation in chicken adipocytes via different mechanisms.

Adipocyte 2020 12;9(1):120-131

College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, China.

The present study investigated the effects of varying concentrations of sodium butyrate (SB) on fat accumulation and cell proliferation in chicken adipocytes. High and low serial concentrations of SB used significantly reduced adipocytic fat accumulation. However, they were observed to exhibit differences in cell morphology and distinctions in lipogenic genes expression profiles. Read More

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http://dx.doi.org/10.1080/21623945.2020.1738791DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153540PMC
December 2020

Antibody-mediated neutralization of ACBP/DBI has anorexigenic and lipolytic effects.

Adipocyte 2020 12;9(1):116-119

Metabolomics and Cell Biology Platforms, Gustave Roussy Cancer Campus, Villejuif, France.

We recently identified acyl coenzyme A-binding protein (ACBP)/diazepam binding inhibitor (DBI) as a novel 'hunger factor': a protein that is upregulated in human or murine obesity and that, if administered to mice, causes hyperphagy, adipogenesis and obesity. Conversely, neutralization of ACBP/DBI by systemic injection of neutralizing monoclonal antibodies or autoantibodies produced after auto-immunization against ACBP/DBI has anorexigenic and lipolytic effects. Thus, neutralization of ACBP/DBI results in reduced food intake subsequent to the activation of anorexigenic neurons and the inactivation of orexigenic neurons in the hypothalamus. Read More

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http://dx.doi.org/10.1080/21623945.2020.1736734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153538PMC
December 2020

Lifestyle intervention in individuals with impaired glucose regulation affects Caveolin-1 expression and DNA methylation.

Adipocyte 2020 12;9(1):96-107

Faculty of Biology, Medicine and Health and Manchester Academic Health Sciences Centre, University of Manchester, Manchester, UK.

Aims: We investigated whether a lifestyle intervention could influence expression and DNA methylation of diabetes-related genes in patients with impaired glucose regulation (IGR), the results were compared to bariatric surgery, considering it an intensive change.

Methods: Twenty participants with IGR had adipose tissue biopsy and blood collected pre- and post-lifestyle (6 months) intervention; 12 obese patients had subcutaneous fat taken before and after bariatric surgery. RNA/DNA was extracted from all samples and underwent qPCR. Read More

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http://dx.doi.org/10.1080/21623945.2020.1732513DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153542PMC
December 2020

Introduction of ligated vessels promote the retention and regeneration of free fat: constructing a fat flap in tissue engineering chamber.

Adipocyte 2020 12;9(1):108-115

Department of Plastic and Cosmetic Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, P.R. China.

: Breast reconstruction with fat grafting has an unstable retention rate due to insufficient revascularization. Tissue Engineering Chamber (TEC) model can promote tissue regeneration in the chamber by introducing ligated vessels around the tissue. We introduced ligated vessels with free fat graft to investigate the retention rate and revascularization of grafted fat that in TEC model. Read More

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http://dx.doi.org/10.1080/21623945.2020.1735025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153550PMC
December 2020

Activated brown adipose tissue and its relationship to adiposity and metabolic markers: an exploratory study.

Adipocyte 2020 12;9(1):87-95

Division of Endocrinology, Metabolism and Molecular Medicine, Northwestern University, Feinberg School of Medicine, Chicago, IL, USA.

: To explore relationships between PET/CT characteristics of cold-activated brown adipose tissue (BAT), measures of adiposity and metabolic markers.: We conducted a post-hoc analysis of a study which utilized PET/CT to characterize BAT. 25 men ages 18-24 (BMI 19. Read More

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http://dx.doi.org/10.1080/21623945.2020.1724740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039639PMC
December 2020

The rs4994 polymorphism increases risk of childhood and adolescent overweight/obesity for East Asia's population: an evidence-based meta-analysis.

Adipocyte 2020 12;9(1):77-86

Department of Cell Biology, School of Biology and Basic Medical Science, Soochow University, Suzhou, Jiangsu Province, China.

Whether the Adrenoceptor Beta 3 () gene rs4994 polymorphism could affect the individual risk of childhood and adolescent overweight/obesity remains controversial. This meta-analysis was performed to estimate the prevalence of this polymorphism in overweight/obesity, and test the potential association by summarizing existing evidence. Comprehensive literature search in PubMed, Web of Science, Cochrane Library, Wanfang, and CNKI databases was performed to identify eligible data sets. Read More

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http://dx.doi.org/10.1080/21623945.2020.1722549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999834PMC
December 2020

Matrix Gla protein regulates adipogenesis and is serum marker of visceral adiposity.

Adipocyte 2020 12;9(1):68-76

Center for Translational Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, People's Republic of China.

Matrix Gla protein (MGP) is a potent calcification inhibitor. -/- mice display increased proportion of brown adipose tissue. However, whether MGP is involved in fat metabolism remains unclear. Read More

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http://dx.doi.org/10.1080/21623945.2020.1721692DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999844PMC
December 2020

Transplantation of brown adipose tissue up-regulates miR-99a to ameliorate liver metabolic disorders in diabetic mice by targeting NOX4.

Adipocyte 2020 12;9(1):57-67

Department of Endocrinology & Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.

Nonalcoholic fatty liver disease (NAFLD), main cause of liver damage, is inextricably linked to diabetes. However, there is no specific means to improve the pathology of fatty liver in diabetic patients. Brown adipose tissue (BAT) is an important endocrine organ that secretes adipokines and microRNAs (miRNAs) involved in systemic metabolic regulation. Read More

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http://dx.doi.org/10.1080/21623945.2020.1721970DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999837PMC
December 2020

Optimizing adipogenic transdifferentiation of bovine mesenchymal stem cells: a prominent role of ascorbic acid in induction.

Adipocyte 2020 12;9(1):35-50

Institute of Veterinary-Physiology, Freie Universität Berlin, Berlin, Germany.

Adipocyte differentiation of bovine adipose-derived stem cells (ASC) was induced by foetal bovine serum (FBS), biotin, pantothenic acid, insulin, rosiglitazone, dexamethasone and 3-isobutyl-1-methylxanthine, followed by incubation in different media to test the influence of ascorbic acid (AsA), bovine serum lipids (BSL), FBS, glucose and acetic acid on transdifferentiation into functional adipocytes. Moreover, different culture plate coatings (collagen-A, gelatin-A or poly-L-lysine) were tested. The differentiated ASC were subjected to Nile red staining, DAPI staining, immunocytochemistry and quantitative reverse transcription PCR (for ). Read More

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http://dx.doi.org/10.1080/21623945.2020.1720480DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999845PMC
December 2020

Association of rs9939609 polymorphism with serum leptin, insulin, adiponectin, and lipid profile in overweight adults.

Adipocyte 2020 12;9(1):51-56

Department of Clinical Nutrition, School of Nutrition & Food Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.

gene polymorphisms are associated with obesity and food intake. This study aimed to investigate the association of rs9939609 polymorphism genotypes with serum glucose, lipid profile and serum hormones level. This cross-sectional study was carried out on 196 randomly selected overweight adults. Read More

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http://dx.doi.org/10.1080/21623945.2020.1722550DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999843PMC
December 2020

Insulin negatively regulates dedifferentiation of mouse adipocytes in vitro.

Adipocyte 2020 12;9(1):24-34

Shandong Provincial Key Laboratory of Animal Resistant Biology, College of Life Sciences, Shandong Normal University, Jinan, China.

Insulin plays an important role during adipogenic differentiation of animal preadipocytes and the maintenance of mature phenotypes. However, its role and mechanism in dedifferentiation of adipocyte remains unclear. This study investigated the effects of insulin on dedifferentiation of mice adipocytes, and the potential mechanisms. Read More

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http://dx.doi.org/10.1080/21623945.2020.1721235DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999839PMC
December 2020

Differences in fatty acid profiles and desaturation indices of abdominal subcutaneous adipose tissue between pregnant women with and without PCOS.

Adipocyte 2020 12;9(1):16-23

Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

The objective was to determine the differences in fatty acid (FA) profiles in subcutaneous adipose tissue (AT) between pregnant women with polycystic ovary syndrome (PCOS) and those without PCOS. FA profiles of AT samples from 13 PCOS and 32 non-PCOS, all of whom underwent caesarean section were compared using gas chromatography. Age and BMI in the two groups were similar. Read More

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http://dx.doi.org/10.1080/21623945.2019.1710021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6959300PMC
December 2020

Expanding the role of bitter taste receptor in extra oral tissues: TAS2R38 is expressed in human adipocytes.

Adipocyte 2020 12;9(1):7-15

Laboratorio di ricerche sull'Obesità, Istituto Auxologico Italiano, IRCCS, Milano, Italy.

Increasing evidence indicates that taste receptors mediate a variety of functions in extra-oral tissues. The present study investigated the expression of bitter taste receptor TAS2R38 in human adipocytes, the possible link with genetic background and the role of TAS2R38 in cell delipidation and lipid accumulation rate . Subcutaneous (SAT) and visceral (VAT) adipose tissues were collected in 32 obese and 18 lean subjects. Read More

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http://dx.doi.org/10.1080/21623945.2019.1709253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6959282PMC
December 2020

Unspecific DNA recombination in AdipoqCre-ER - mediated knockout approaches in transgenic mice is sex-, age- and genotype-dependent.

Adipocyte 2020 12;9(1):1-6

Institute of Anatomy and Cell Biology, Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany.

Due to the epidemic rise of obesity prevalence, adipose tissue (AT) research is of major interest. Our aim was to study specificity of the most-common Cre/loxP approach for inducible gene manipulation of AT in mice (AdipoqCre-ER). We used mice with tamoxifen-sensitive Cre recombinase controlled by the adiponectin promoter (AdipoqCre-ER), which were crossed to a tdTomato reporter mouse to visualize the site of recombination on a single-cell resolution. Read More

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http://dx.doi.org/10.1080/21623945.2019.1701394DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6959310PMC
December 2020

Relationship between epicardial adipose tissue thickness and epicardial adipocyte size with increasing body mass index.

Adipocyte 2019 12;8(1):412-420

Department of Physiology, HeartOtago, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand.

Macroscopic deposition of epicardial adipose tissue (EAT) has been strongly associated with numerous indices of obesity and cardiovascular disease risk. In contrast, the morphology of EAT adipocytes has rarely been investigated. We aimed to determine whether obesity-driven adipocyte hypertrophy, which is characteristic of other visceral fat depots, is found within EAT adipocytes. Read More

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http://dx.doi.org/10.1080/21623945.2019.1701387DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948959PMC
December 2019

Modelling the gene expression and the DNA-binding in the 3T3-L1 differentiating adipocytes.

Adipocyte 2019 12;8(1):401-411

Department of Biochemistry and Convergence Medical Sciences and Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, Republic of Korea.

The 3T3-L1 pre-adipocyte cell line is widely used to study the fat cell differentiation . Researchers also use this cell model to study obesity and insulin resistance. We surveyed the literature, the gene expression omnibus and the sequence read archive for RNA-Seq and ChIP-Seq datasets of MDI-induced 3T3-L1 differentiating cells sampled at one or more time points. Read More

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http://dx.doi.org/10.1080/21623945.2019.1697563DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948977PMC
December 2019

Non-obesogenic doses of palmitate disrupt circadian metabolism in adipocytes.

Adipocyte 2019 12;8(1):392-400

Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.

Saturated fatty acids, such as palmitate, lead to circadian disruption. We aimed at studying the effect of low doses of palmitate on circadian metabolism and to decipher the mechanism by which fatty acids convey their effect in adipocytes. Mice were fed non-obesogenic doses of palm or olive oil and adipocytes were treated with palmitate and oleate. Read More

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http://dx.doi.org/10.1080/21623945.2019.1698791DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948973PMC
December 2019

Interactions between macro-nutrients' intake, FTO and IRX3 gene expression, and FTO genotype in obese and overweight male adolescents.

Adipocyte 2019 12;8(1):386-391

Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, USA.

This study is the first to identify the effects of FTO genotype on the interactions between the level of macro-nutrients intake and the expression level of fat mass and obesity associated (FTO) and homeobox transcription factor iriquois-3 (IRX3) genes This longitudinal study was carried out on 84 overweight and obese adolescent boys in Tehran, Iran. The rs9930506 SNP in was genotyped at baseline and the level of and expression in PBMCs and macro-nutrients' intake were assessed at baseline and after 18 weeks of the intervention. The results identified that the higher carbohydrates intake significantly up-regulated the FTO gene (P = 0. Read More

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http://dx.doi.org/10.1080/21623945.2019.1693745DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948981PMC
December 2019

Adipocyte epigenetic alterations and potential therapeutic targets in transgenerationally inherited lean and obese phenotypes following ancestral exposures.

Adipocyte 2019 12;8(1):362-378

Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA, USA.

The incidence of obesity has increased dramatically over the past two decades with a prevalence of approximately 40% of the adult population within the United States. The current study examines the potential for transgenerational adipocyte (fat cell) epigenetic alterations. Adipocytes were isolated from the gonadal fat pad of the great-grand offspring F3 generation 1-year old rats ancestrally exposed to DDT (dichlorodiphenyltrichloroethane), atrazine, or vehicle control in order to obtain adipocytes for DNA methylation analysis. Read More

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http://dx.doi.org/10.1080/21623945.2019.1693747DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948971PMC
December 2019

A-FABP and oestrogens are independently involved in the development of breast cancer.

Adipocyte 2019 12;8(1):379-385

Division of Cancer Prevention, National Cancer Institute, 9609 Medical Center Drive, Rockville, MD, USA.

We previously reported that postmenopausal obese women exhibit increased levels of circulating adipocyte fatty acid binding protein (A-FABP), which is associated with breast cancer (BC) development. In postmenopause, increased oestrogen levels are reported to be associated with increased BC risk. Herein, we assessed if oestrogens, including oestrone (E1), oestradiol (E2) and oestriol (E3), are associated with A-FABP in the obesity-related BC development. Read More

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http://dx.doi.org/10.1080/21623945.2019.1690827DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948962PMC
December 2019

An easy method for the clear detection of beige fat UCP1 by Western blotting.

Adipocyte 2019 12;8(1):357-361

Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University, Gwangju, Korea.

Beige adipocytes, which consume energy mainly in an uncoupling protein 1 (UCP1)-dependent manner, are risen in white adipose tissue (WAT) depots. Since beige adipocyte development is gaining attention as a potential strategy for conquering obesity, worldwide researchers are making efforts to study its biological aspects. However, assessing UCP1 protein levels in beige adipocytes is challenging because of the high level of lipid contaminants in WAT. Read More

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http://dx.doi.org/10.1080/21623945.2019.1693746DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948967PMC
December 2019

Increased oxidative stress, inflammation and fibrosis in perirenal adipose tissue of patients with cortisol-producing adenoma.

Adipocyte 2019 12;8(1):347-356

Department of Endocrinology and Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Although much is known about that corticosteroids affect the functions of adipose tissues, little genetic information is available for perirenal adipose tissue (peri-N) from patients with cortisol-producing adenoma (CPA). We conducted microarray analysis of peri-N from patients with CPA by using an Affymetrix human U133 plus 2.0 array. Read More

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http://dx.doi.org/10.1080/21623945.2019.1690834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948963PMC
December 2019

Visceral adipose tissue alteration of expression is associated with gestational diabetes but not promoter DNA methylation.

Adipocyte 2019 12;8(1):339-346

Division of 'Experimental Obstetrics', Clinic of Obstetrics, Charité - Universitätsmedizin Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health , Berlin , Germany.

Obesity and diabetes are at an epidemic rate, as well as growing incidences of gestational diabetes mellitus (GDM) which causes pregnancy risks, and harm in both maternal and child health. It remains unclear which molecular mechanisms are driving the functional differences between visceral and subcutaneous fat and how these types directly affect an individual's health outcome. Paired abdominal subcutaneous and omental visceral adipose tissue were collected from women with GDM (n = 20) and with normal glucose tolerance (NGT, n = 22) during planned caesarian section. Read More

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http://dx.doi.org/10.1080/21623945.2019.1675239DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948979PMC
December 2019
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Quantitative analyses of adiposity dynamics in zebrafish.

Adipocyte 2019 12;8(1):330-338

a Centre for Cardiovascular Science, University of Edinburgh , Edinburgh EH16 4TJ , UK.

Adipose tissues often exhibit subtle, quantitative differences between individuals, leading to a graded series of adiposity phenotypes at the population level. Robust, quantitative analyses are vital for studying these differences. In this Commentary we highlight two articles from our lab that employ sensitive new methods in zebrafish capable of delineating complex and quantitative adiposity phenotypes. Read More

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http://dx.doi.org/10.1080/21623945.2019.1648175DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768273PMC
December 2019
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Identification of biomarkers, pathways and potential therapeutic agents for white adipocyte insulin resistance using bioinformatics analysis.

Adipocyte 2019 12;8(1):318-329

a Department of Pathology and Pathophysiology, Wuhan University School of Basic Medical Sciences , Wuhan , China.

For the better understanding of insulin resistance (IR), the molecular biomarkers in IR white adipocytes and its potential mechanism, we downloaded two mRNA expression profiles from Gene Expression Omnibus (GEO). The white adipocyte samples in two databases were collected from the human omental adipose tissue of IR obese (IRO) subjects and insulin-sensitive obese (ISO) subjects, respectively. We identified 86 differentially expressed genes (DEGs) between the IRO and ISO subjects using limma package in R software. Read More

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http://dx.doi.org/10.1080/21623945.2019.1649578DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768254PMC
December 2019

Roles of omental and bone marrow adipocytes in tumor biology.

Adipocyte 2019 12;8(1):304-317

a Department of Pathology, Yonsei University College of Medicine , Seoul , South Korea.

Accumulating evidence highlights the importance of interactions between tumour cells and stromal cells for tumour initiation, progression, and metastasis. In tumours that contain adipocyte in their stroma, adipocytes contribute to modification of tumour microenvironment and affect metabolism of tumour and tumour progression by production of cytokines and adipokines from the lipids. The omentum and bone marrow (BM) are highly adipocyte-rich and are also common metastatic and primary tumour developmental sites. Read More

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http://dx.doi.org/10.1080/21623945.2019.1643189DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768257PMC
December 2019
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Low-intensity pulsed ultrasound inhibits adipogenic differentiation via HDAC1 signalling in rat visceral preadipocytes.

Adipocyte 2019 12;8(1):292-303

a Department of Cardiology , The First Affiliated Hospital of Nanjing Medical University , Nanjing , Jiangsu , China.

Non-drug strategy targeting adipocyte differentiation is critical for alleviating visceral obesity and its related diseases. However, whether and how low intensity pulsed ultrasound (LIPUS) could be used for inhibiting visceral adipocyte differentiation is not fully understood. In this study, we aim to investigate the effect and associated mechanism of LIPUS on primary visceral preadipocyte differentiation and explore its potential role for clinical visceral obesity management. Read More

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http://dx.doi.org/10.1080/21623945.2019.1643188DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768184PMC
December 2019
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Insulin-induced lipid body accumulation is accompanied by lipid remodelling in model mast cells.

Adipocyte 2019 12;8(1):265-279

b Laboratory of Experimental Medicine , John A. Burns School of Medicine, University of Hawai'i , Honolulu , HI , USA.

Mast cell lipid bodies are key to initiation, maintenance and resolution of inflammatory responses in tissue. Mast cell lines, primary bone marrow-derived mast cells and peripheral blood basophils present a 'steatotic' phenotype in response to chronic insulin exposure, where cells become loaded with lipid bodies. Here we show this state is associated with reduced histamine release, but increased capacity to release bioactive lipids. Read More

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http://dx.doi.org/10.1080/21623945.2019.1636624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768188PMC
December 2019
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A new laminopathy caused by an Arg133/Leu mutation in and the effects thereof on adipocyte differentiation and the transcriptome.

Adipocyte 2019 12;8(1):280-291

a Department of Endocrinology , The Affiliated Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University , Hangzhou , Zhejiang , China.

We report a new laminopathy that includes generalized lipoatrophy, insulin-resistant diabetes, micrognathia and biopsy-proven, focal segmental glomerulosclerosis in a female, caused by a de novo heterozygous mutation R133L in the lamin A/C gene (). We analysed the nuclear morphology and laminar distribution in 3T3-L1 pre-adipocytes overexpressing human wild-type lamin A/C ( WT) or lamin A/C with the R133L mutation ( R133L). We found the nuclear size was varied, nuclear membrane invagination or blebbing, and an irregular A-type lamin meshwork in cells overexpressing R133L. Read More

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http://dx.doi.org/10.1080/21623945.2019.1640007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768263PMC
December 2019
2 Reads

Influence of glucose and insulin in human adipogenic differentiation models with adipose-derived stem cells.

Adipocyte 2019 12;8(1):254-264

a Department of Plastic, Aesthetic, Hand and Reconstructive Surgery , Hannover Medical School , Hanover , Germany.

Autologous fat grafting represents an attractive source for tissue engineering applications in the field of reconstructive medicine. However, in adipogenic differentiation protocols for human adipose-derived stem cells, the concentration of glucose and insulin varies considerably. With the intent to gain maximum tissue augmentation, we focused on the late phase of adipogenesis. Read More

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http://dx.doi.org/10.1080/21623945.2019.1636626DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768274PMC
December 2019
1 Read