41,895 results match your criteria Adenoviruses

Morbidity burden, seasonality and factors associated with the human respiratory syncytial virus, human parainfluenza virus, and human adenovirus infections in Kenya.

IJID Reg 2021 Dec 9;1:72-78. Epub 2021 Oct 9.

Centre for Virus Research, Kenya Medical Research Institute, Nairobi, Kenya.

Background: Human respiratory syncytial viruses (HRSV), human parainfluenza viruses (HPIV), and human adenoviruses (HAdVs) cause a substantial morbidity burden globally.

Objective: We sought to estimate morbidity burden, assess seasonality, and determine factors associated with these respiratory viruses in Kenya.

Methods: The data were obtained from Kenyan sites included in the Köppen-Geiger climate classification system. Read More

View Article and Full-Text PDF
December 2021

Role of Adenoviruses in Cancer Therapy.

Front Oncol 2022 9;12:772659. Epub 2022 Jun 9.

Lecturer of Biomedical Sciences, Department of Biology, College of Natural and Computational Sciences, Arba Minch University, Arba Minch, Ethiopia.

Cancer is one of the leading causes of death in the world, which is the second after heart diseases. Adenoviruses (Ads) have become the promise of new therapeutic strategy for cancer treatment. The objective of this review is to discuss current advances in the applications of adenoviral vectors in cancer therapy. Read More

View Article and Full-Text PDF

Intestinal Microbiota Participates in the Protective Effect of HO-1/BMMSCs on Liver Transplantation With Steatotic Liver Grafts in Rats.

Front Microbiol 2022 10;13:905567. Epub 2022 Jun 10.

Department of Organ Transplantation, Tianjin First Central Hospital, Tianjin, China.

The present study aimed to explore whether heme oxygenase-1 (HO-1)-modified bone marrow mesenchymal stem cells (BMMSCs) have a protective effect on liver transplantation with steatotic liver grafts in rats, and to determine the role of the intestinal microbiota in such protection. HO-1/BMMSCs were obtained by transduction of gene [encoding heme oxygenase (HO-1)]-encoding adenoviruses into primary rat BMMSCs. Steatotic livers were obtained by feeding rats a high-fat diet, and a model of liver transplantation with steatotic liver grafts was established. Read More

View Article and Full-Text PDF

Adenoviral-Vectored Centralized Consensus Hemagglutinin Vaccine Provides Broad Protection against H2 Influenza a Virus.

Vaccines (Basel) 2022 Jun 10;10(6). Epub 2022 Jun 10.

Nebraska Center for Virology, School of Biological Sciences, University of Nebraska-Lincoln, 4240 Fair Street, Lincoln, NE 68504, USA.

Several influenza pandemics have occurred in the past century, one of which emerged in 1957 from a zoonotic transmission of H2N2 from an avian reservoir into humans. This pandemic caused 2-4 million deaths and circulated until 1968. Since the disappearance of H2N2 from human populations, there has been waning immunity against H2, and this subtype is not currently incorporated into seasonal vaccines. Read More

View Article and Full-Text PDF

Differential Viral Genome Diversity of Healthy and RSS-Affected Broiler Flocks.

Microorganisms 2022 May 25;10(6). Epub 2022 May 25.

Institute of Virology, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland.

The intestinal virus community contributes to health and disease. Runting and stunting syndrome (RSS) is associated with enteric viruses and leads to economic losses in the poultry industry. However, many viruses that potentially cause this syndrome have also been identified in healthy animals. Read More

View Article and Full-Text PDF

Hepatitis of Unknown Origin and Etiology (Acute Non HepA-E Hepatitis) among Children in 2021/2022: Review of the Current Findings.

Healthcare (Basel) 2022 May 24;10(6). Epub 2022 May 24.

Department of Translational Medicine, Faculty of Medicine, Lund University, 22362 Malmö, Sweden.

Several clusters and individual cases of acute hepatitis have been reported in the US, Europe and recently in Asia and Central America since October 2021. A laboratory investigation of the common viral hepatitis agents (HAV, HBV, HCV, HDV and HEV) yielded negative results prompting the use of the term "acute non HepA-E hepatitis" to describe this condition. The cases were characterized by the manifestations of acute hepatitis (abdominal pain, vomiting, diarrhea, jaundice and very high levels of liver enzymes) affecting children with a median age of 3-4 years. Read More

View Article and Full-Text PDF

A qPCR-Based Method for Quantification of Replication Competent Adenovirus (RCA) in Conditionally Replicating Oncolytic Adenoviruses.

Methods Mol Biol 2022 ;2521:249-258

Department of Immunology, Genetics and Pathology; Science for Life Laboratory, Uppsala University, Uppsala, Sweden.

Production of conditionally replicating adenoviruses may unfortunately generate undesired replication-competent adenovirus (RCA) which raises safety concerns in clinical usage. Cell-based assays can detect RCA in batches of nonreplicating adenoviral vectors but cannot distinguish RCA from conditionally replicating oncolytic adenoviruses. Considering the great potential in using oncolytic viruses for cancer treatment, there is a need for comprehensive RCA-detection and -quantification methods. Read More

View Article and Full-Text PDF

A dopamine antagonist, domperidone enhances the replication of an oncolytic adenovirus in human tumour cells.

J Gen Virol 2022 Jun;103(6)

Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.

Oncolytic adenoviruses (OAds) have attracted much attention as novel anticancer agents. Numerous studies have examined the antitumour effects of combinational use of an OAd and anticancer agents; however, few chemical compounds enhancing OAd infection have been reported. In this study, we screened a food and drug administration (FDA)-approved drug library containing 1134 small chemical compounds to identify chemical compounds that enhance OAd replication in human tumour cells. Read More

View Article and Full-Text PDF

SET improved oocyte maturation by serine/threonine protein phosphatase 2A and inhibited oocyte apoptosis in mouse oocytes.

Reprod Biol 2022 Jun 18;22(3):100668. Epub 2022 Jun 18.

State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.

SET is a multifunctional protein involved in a variety of molecular processes such as cell apoptosis and cell-cycle regulation. In ovaries SET is predominantly expressed in theca cells and oocytes. In polycystic ovary syndrome (PCOS) patients the expression of SET was increased than healthy people. Read More

View Article and Full-Text PDF

The First Occurrence of Hepatitis-Hydropericardium Syndrome in Iran and Effective Applied Control Measures in the Affected Commercial Broiler Flock.

Avian Dis 2022 Jun 15. Epub 2022 Jun 15.

Veterinary Head Office of Khorasan Razavi province, 9185333446, Mashhad, Iran.

Fowl adenoviruses cause three economically important diseases in broiler chicken flocks: hepatitis-hydropericardium syndrome (HHS), inclusion body hepatitis (IBH), and adenoviral gizzard erosion. IBH has not been considered a serious threat in northeast Iran since the last decade, because no major effect on flock performance has been noticed along with a low mortality rate. During this period, all the sporadic IBH outbreaks have also been investigated for HHS without finding any confirmed case. Read More

View Article and Full-Text PDF

Preparation and identification of a single domain antibody specific for adenovirus vectors and its application to the immunoaffinity purification of adenoviruses.

AMB Express 2022 Jun 20;12(1):80. Epub 2022 Jun 20.

College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 29, Erdos East Street, Saihan District, Huhhot, 010010, Inner Mongolia, China.

Adenovirus belongs to the family of Adenoviridae. As a vaccine carrier, it has high safety and stimulates the body to produce cellular immunity and humoral immunity. This study prepared an adenoviral vector-specific single-domain antibody for use in adenovirus identification and purification. Read More

View Article and Full-Text PDF

Infectious Pepper Mild Mottle Virus and Human Adenoviruses as Viral Indices in Sewage and Water Samples.

Food Environ Virol 2022 Jun 17. Epub 2022 Jun 17.

Botany and Microbiology Department, Faculty of Science, Cairo University, Cairo, Egypt.

The objective of this study was to compare human adenoviruses (HAdVs) genome and infectivity, polyomaviruses (JC and BK) genome (JCPyVs) and (BKPyVs), Pepper Mild Mottle Virus (PMMoV) genome and infectivity, and infectious bacteriophages as viral indices for sewage and water samples. One hundred and forty-four samples were collected from inlets and outlets of water and wastewater treatment plants (WTPs), and WWTPs within Greater Cairo from October 2015 till March 2017. Two methods of viral concentration [Aluminium hydroxide (Al(OH)) precipitation method and adsorption-elution technique followed by organic flocculation method] were compared to determine which of them was the best method to concentrate viruses from sewage and water. Read More

View Article and Full-Text PDF

Adenovirus-Vectored African Swine Fever Virus pp220 Induces Robust Antibody, IFN-γ, and CTL Responses in Pigs.

Front Vet Sci 2022 31;9:921481. Epub 2022 May 31.

Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS, United States.

African Swine Fever Virus (ASFV) poses a serious threat to the pork industry worldwide; however, there is no safe vaccine or treatment available. The development of an efficacious subunit vaccine will require the identification of protective antigens. The ASFV pp220 polyprotein is essential for virus structural integrity. Read More

View Article and Full-Text PDF

Praja1 RING-finger E3 ubiquitin ligase is a common suppressor of neurodegenerative disease-associated protein aggregation.

Neuropathology 2022 Jun 14. Epub 2022 Jun 14.

Division of Pathological Neuroscience, Department of Pathology, Tokyo Women's Medical University, Tokyo, Japan.

The formation of misfolded protein aggregates is one of the pathological hallmarks of neurodegenerative diseases. We have previously demonstrated the cytoplasmic aggregate formation of adenovirally expressed transactivation response DNA-binding protein of 43 kDa (TDP-43), the main constituent of neuronal cytoplasmic aggregates in cases of amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD), in cultured neuronal cells under the condition of proteasome inhibition. The TDP-43 aggregate formation was markedly suppressed by co-infection of adenoviruses expressing heat shock transcription factor 1 (HSF1), a master regulator of heat shock response, and Praja1 RING-finger E3 ubiquitin ligase (PJA1) located downstream of the HSF1 pathway. Read More

View Article and Full-Text PDF

COVID-19 vaccine-induced thrombotic thrombocytopenia: Adverse event associated with adenovirus vector-based vaccines.

Can Fam Physician 2022 Jun;68(6):434-437

Research Assistant at the Bruyère Research Institute and at the University of Ottawa.

View Article and Full-Text PDF

The application of lentiviral vectors for the establishment of TGFβ2-induced ocular hypertension in C57BL/6J mice.

Exp Eye Res 2022 Jun 9;221:109137. Epub 2022 Jun 9.

Eugene & Marilyn Glick Eye Institute, Department of Ophthalmology, Indiana University School of Medicine, USA; Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, USA; Department of Pharmacology and Toxicology, Indiana University School of Medicine, USA. Electronic address:

Elevated levels of TGFβ2 in the aqueous humor is associated with the pathological changes in the trabecular meshwork (TM). These changes lead to ocular hypertension (OHT), the most important risk factor for the development and progression of primary open angle glaucoma (POAG), a leading cause of blindness worldwide. Therefore, TGFβ2 is frequently used to develop OHT models including in perfusion cultured eyes and in mouse eyes. Read More

View Article and Full-Text PDF

The Clinical Risk Factors of Adenovirus Pneumonia in Children Based on the Logistic Regression Model: Correlation with Lactate Dehydrogenase.

Int J Clin Pract 2022 24;2022:3001013. Epub 2022 Mar 24.

Department of Infectious Diseases, Tianjin Children's Hospital, 238 Longyan Road, Beichen District, Tianjin, China.

Methods: Children with bacterial pneumonia (41 cases) and adenovirus pneumonia (179 cases) hospitalized in Tianjin Children's Hospital from January to October 2020 were selected. The differences in baseline and clinical characteristics between children with two pneumonias, respectively, were compared via the chi-square test and Wilcox test. The Least Absolute Shrinkage and Selection Operator (LASSO) model was applied to screen the pneumonia type-related characteristics. Read More

View Article and Full-Text PDF

HydrAd: A Helper-Dependent Adenovirus Targeting Multiple Immune Pathways for Cancer Immunotherapy.

Cancers (Basel) 2022 Jun 2;14(11). Epub 2022 Jun 2.

Department of Medicine, Section of Hematology/Oncology, Baylor College of Medicine, Houston, TX 77030, USA.

For decades, Adenoviruses (Ads) have been staple cancer gene therapy vectors. Ads are highly immunogenic, making them effective adjuvants. These viruses have well characterized genomes, allowing for substantial modifications including capsid chimerism and therapeutic transgene insertion. Read More

View Article and Full-Text PDF

Discovery of novel DNA viruses in small mammals from Kenya.

Virol Sin 2022 Jun 6. Epub 2022 Jun 6.

CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China; Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, 430074, China. Electronic address:

Emergence and re-emergence of infectious diseases of wildlife origin have led pre-emptive pathogen surveillances in animals to be a public health priority. Rodents and shrews are among the most numerically abundant vertebrate taxa and are known as natural hosts of important zoonotic viruses. Many surveillance programs focused more on RNA viruses. Read More

View Article and Full-Text PDF

Cellular protein HSC70 promotes fowl adenovirus serotype 4 replication in LMH cells via interacting with viral 100K protein.

Poult Sci 2022 Jul 4;101(7):101941. Epub 2022 May 4.

College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China. Electronic address:

Fowl adenovirus serotype 4 (FAdV-4), the predominant causative agent of hepatitis-hydropericardium syndrome (HHS), has caused severe economic losses to poultry industry since 2015. Although fiber2 and hexon have been confirmed to be the virulence-related factors, the roles of nonstructural viral proteins in pathogenicity of FAdV-4 remain poorly understood. Here, a tandem mass spectrometry (MS) was used to identify host factors interacted with 100K protein of hypervirulent FAdV-4 isolate (CH/HNJZ/2015), and 2595 cellular proteins associated with many biological processes and pathways were identified according to Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses. Read More

View Article and Full-Text PDF

A qPCR-Based Method for Quantification of RCA Contaminants in Oncolytic Adenovirus Products.

Front Mol Biosci 2022 23;9:883249. Epub 2022 May 23.

Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden.

Oncolytic adenovirus is one of the most promising treatments against cancer and is widely evaluated clinically. During high titer production, "Wild-type-" like replication-competent adenovirus (RCA) contaminants can be generated through recombination events due to the DNA sequence similarity between oncolytic virus and host cells. These RCA contaminants raise various safety concerns in clinics. Read More

View Article and Full-Text PDF

Advances in Vaccine Development of the Emerging Novel Genotype Fowl Adenovirus 4.

Front Immunol 2022 20;13:916290. Epub 2022 May 20.

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin, China.

Fowl adenovirus (FAdV) was first reported in Angara Goth, Pakistan, in 1987. For this reason, it is also known as "Angara disease." It was later reported in China, Japan, South Korea, India, the United States, Canada, and other countries and regions, causing huge economic losses in the poultry industry worldwide. Read More

View Article and Full-Text PDF

Oncolytic Adenoviral Vector-Mediated Expression of an Anti-PD-L1-scFv Improves Anti-Tumoral Efficacy in a Melanoma Mouse Model.

Front Oncol 2022 20;12:902190. Epub 2022 May 20.

Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Naples, Italy.

Oncolytic virotherapy is an emerging therapeutic approach based on replication-competent viruses able to selectively infect and destroy cancer cells, inducing the release of tumor-associated antigens and thereby recruiting immune cells with a subsequent increase in antitumoral immune response. To increase the anticancer activity, we engineered a specific oncolytic adenovirus expressing a single-chain variable fragment of an antibody against PD-L1 to combine blockage of PD-1/PD-L1 interaction with the antitumoral activity of Onc.Ad5. Read More

View Article and Full-Text PDF

Development of a Rapid Immuno-Based Screening Assay for the Detection of Adenovirus in Eye Infections.

ACS Omega 2022 May 19;7(21):17555-17562. Epub 2022 May 19.

Alfaisal University, Al Zahrawi Street, Al Maather, Al Takhassusi Rd, Riyadh 11533, Saudi Arabia.

Despite progress in fighting infectious diseases, human pathogenesis and death caused by infectious diseases remain relatively high worldwide exceeding that of cancer and cardiovascular diseases. Human adenovirus (HAdV) infects cells of the upper respiratory tract causing flu-like symptoms that are accompanied by pain and inflammation. Diagnosis of HAdV is commonly achieved by conventional methods such as viral cultures, immunoassays, and polymerase chain reaction (PCR) techniques. Read More

View Article and Full-Text PDF

A screening of wild bird samples enhances our knowledge about the biodiversity of avian adenoviruses.

Vet Res Commun 2022 Jun 6. Epub 2022 Jun 6.

Veterinary Medical Research Institute, Eötvös Loránd Research Network, Budapest, Hungary.

Wild birds are threatened by anthropic effects on a global scale, and their adenoviruses might contribute to their endangerment. Thus, it is important to reveal the real biodiversity of avian adenoviruses, as, unfortunately, this research topic is far from being prioritized. The turkey hemorrhagic enteritis is an economically important disease causing high mortalities, and its causative siadenoviral agent is only distantly related to other avian siadenoviruses in phylogenetic analyses. Read More

View Article and Full-Text PDF

Development of an adenovirus-mediated reporter assay system to detect a low concentration of retinoic acid in MCF-7 cells.

J Toxicol Sci 2022 ;47(6):249-255

Laboratory of Environmental and Health Sciences, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University.

Retinoic acid, an active form of vitamin A, plays very important roles in mammalian embryogenesis. The concentration of retinoic acid is extremely low and strictly regulated by enzymes of cytochrome P450 (CYP) family, CYP26s (CYP26A1, CYP26B1 and CYP26C1) in the cells. Therefore, it is thought that changes in CYP26s activities due to exposure to a wide variety of drugs and chemicals exhibit teratogenicity. Read More

View Article and Full-Text PDF

The changing pattern of common respiratory and enteric viruses among outpatient children in Shanghai, China: Two years of the COVID-19 pandemic.

J Med Virol 2022 May 31. Epub 2022 May 31.

Department of Clinical Laboratory, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, China.

Nonpharmaceutical interventions (NPIs) taken to combat the coronavirus disease 2019 (COVID-19) pandemic have not only decreased the spread of severe acute respiratory syndrome coronavirus 2 but also have had an impact on the prevalence of other common viruses. This study aimed to investigate the long-term impact of NPIs on common respiratory and enteric viruses among children in Shanghai, China, as NPIs were relaxed after June 2020. The laboratory results and clinical data of outpatient children with acute respiratory tract infections (ARTI) and acute gastroenteritis (AGE) were analyzed and compared between the post-COVID-19 period (from June 2020 to January 2022) and pre-COVID-19 period (from June 2018 to January 2020). Read More

View Article and Full-Text PDF

Impact of the SARS-CoV-2 Pandemic on the Prevalence and Incidence of Gastrointestinal Viruses in Children up to Five Years Old: a Retrospective Cohort Study.

Microbiol Spectr 2022 May 31:e0266921. Epub 2022 May 31.

Clinical Microbiology and Parasitology Department, Hospital Universitario La Pazgrid.81821.32, Madrid, Spain.

The aim is determining the impact of non-pharmaceutical measures (NPIs) against SARS-CoV-2 in the incidence and prevalence of gastrointestinal viruses (GV) in children. Demographic, analytical, and clinical data of children from which samples were received at the Hospital Universitario La Paz (Madrid, Spain) and that had a gastrointestinal infection with a positive sample through multiplex-PCR for GV were collected. The time periods included were prepandemic (P1): March 14, 2019 to March 14, 2020 and pandemic (P2): March 15, 2020 to March 15, 2021. Read More

View Article and Full-Text PDF

Reduction of Influenza A Virus Transmission in Mice by a Universal Intranasal Vaccine Candidate is Long-Lasting and Does Not Require Antibodies.

J Virol 2022 Jun 31;96(12):e0032022. Epub 2022 May 31.

Division of Cellular and Gene Therapies, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA.

Vaccination against influenza virus infection can protect the vaccinee and also reduce transmission to contacts. Not all types of vaccines induce sterilizing immunity via neutralizing antibodies; some instead permit low-level, transient infection. There has been concern that infection-permissive influenza vaccines may allow continued spread in the community despite minimizing symptoms in the vaccinee. Read More

View Article and Full-Text PDF