Search Our Scientific Publications & Authors

Expert Rev Vaccines 2019 Feb 19. Epub 2019 Feb 19.

a University of Veterinary Medicine (TiHo), Research Center for Emerging Infections and Zoonoses (RIZ) , Bünteweg 17, 30559 Hannover , Germany.

Introduction: Various viruses, including poxviruses, adenoviruses and vesicular stomatitis virus, have been considered as vaccine vectors for the delivery of antigens of interest in the development of vaccines against newly emerging pathogens. Areas covered: Here, we review results that have been obtained with influenza A viruses (IAV) as vaccine vectors. With the advent of reverse genetics technology, IAV-based recombinant vaccine candidates have been constructed that induce protective immunity to a variety of different pathogens of interest, including West Nile virus, Plasmodium falciparum and respiratory syncytial virus. Read More

Int J Endocrinol 2019 15;2019:3054820. Epub 2019 Jan 15.

Department of Pathology and Pathophysiology, Wuhan University School of Basic Medical Sciences, Wuhan, 430071 Hubei, China.

Objective: The involvement of phosphodiesterase type 5 (PDE5) in the development of insulin resistance has been reported recently. However, the underlying molecular mechanism remains unclear. The present study aims at investigating the potential impacts of PDE5A on insulin signaling in C2C12 skeletal muscle myotubes and uncover the related mechanism. Read More

Viruses 2019 Feb 14;11(2). Epub 2019 Feb 14.

Department of Pathology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.

The Zika virus (ZIKV) was first isolated in Africa in 1947. It was shown to be a mild virus that had limited threat to humans. However, the resurgence of the ZIKV in the most recent Brazil outbreak surprised us because it causes severe human congenital and neurologic disorders including microcephaly in newborns and Guillain-Barré syndrome in adults. Read More

Klin Lab Diagn 2018 ;63(10):641-645

«1586 Military Clinical Hospital» of the Ministry of Defense of the Russian Federation, 603105, Nizhniy Novgorod, Russian Federation.

Samples of sputum, blood, bronchoalveolar lavage, swabs from the oropharynx from 255 military personnel undergoing in-patient treatment with an x-ray confirmed diagnosis of community-acquired pneumonia (CAP) were examined by polymerase chain reaction (PCR). The comparison group was included 270 healthy recruits. The detection of S. Read More

Nanoscale 2019 Feb 15. Epub 2019 Feb 15.

Moleculaire Biofysica, Zernike Instituut, Rijksuniversiteit Groningen, The Netherlands.

The ability of adenoviruses to infect a broad range of species has spurred a growing interest in nanomedicine to use adenovirus as a cargo delivery vehicle. While successful maturation of adenovirus and controlled disassembly are critical for efficient infection, the underlying mechanisms regulating these processes are not well understood. Here, we present Atomic Force Microscopy nanoindentation and fatigue studies of adenovirus capsids at different maturation stages to scrutinize their dynamic uncoating properties. Read More

DNA Cell Biol 2019 Feb 14. Epub 2019 Feb 14.

1 Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital, Sichuan University, Chengdu, China.

The shortage of human hepatocytes continues to be a significant limitation for the widespread application of hepatocyte transplantation and bioartificial liver (BAL) support therapy. Recombinant activation gene 2 (Rag2) and fumarylacetoacetate hydrolase (Fah)-deficient mice could be highly repopulated with human hepatocytes. However, Fah/Rag2-deficient mice can only produce up to 1 × 10 human hepatocytes per mouse. Read More

Food Environ Virol 2019 Feb 12. Epub 2019 Feb 12.

Microbiology Section, Department of Biotechnology and Food Science, Universidad de Burgos, Plaza Misael Bañuelos s/n, 09001, Burgos, Spain.

Although the effects of heavy metals on the behavior, including infectivity, of bacteria have been studied, little information is available about their effects on enteric viruses. We report an investigation of effects on the biosynthesis of human adenoviruses (HAdV) and hepatitis A (HAV) of waters contaminated with mineral waste following an environmental disaster in Mariana City, Minas Gerais State, Brazil. The study area was affected on November 5, 2015, by 60 million m of mud (containing very high concentrations of iron salts) from a mining reservoir (Fundão), reaching the Gualaxo do Norte River (sites evaluated in this study), the "Rio Doce" River and finally the Atlantic Ocean. Read More

Viruses 2019 Feb 8;11(2). Epub 2019 Feb 8.

Department of Ophthalmology, Howe Laboratory, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA 02114, USA.

Adenovirus E1A is the first viral protein expressed during infection. E1A controls critical aspects of downstream viral gene expression and cell cycle deregulation, and its function is thought to be highly conserved among adenoviruses. Various bioinformatics analyses of E1A from 38 human adenoviruses of species D (HAdV-D), including likelihood clade model partitioning, provided highly significant evidence of divergence of HAdV-Ds into two distinct groups for the conserved region 3 (CR3), present only in the E1A 13S isoform. Read More

Cancers (Basel) 2019 Feb 8;11(2). Epub 2019 Feb 8.

Division of Basic and Translational Research, Department of Surgery, University of Minnesota, Minneapolis, 55455 MN, USA.

Oncolytic adenoviruses (Ad) are promising tools for cancer therapeutics. Most Ad-based therapies utilize species C serotypes, with Adenovirus type 5 (Ad5) most commonly employed. Prior clinical trials demonstrated low efficiency of oncolytic Ad5 vectors, mainly due to the absence of Ad5 primary receptor (Coxsackie and Adenovirus Receptor, CAR) on cancer cells. Read More

Water Res 2019 Apr 1;153:349-356. Epub 2019 Feb 1.

Department of Paediatrics, University of Alberta, 1405-87 Ave, Edmonton, AB, T6G 1C9, Canada.

The prevalence and seasonal variation of 7 viruses in 6 major rivers in Alberta were assessed using a combination of qPCR, cell culture and integrated cell culture with qPCR (ICC-qPCR). Water samples were collected monthly from rivers at different sites upstream and downstream of major urban centers. Seven viruses including rotavirus, adenovirus, astrovirus, norovirus, sapovirus, JC virus and enterovirus, were detected in at least one of the water samples at each site using qPCR. Read More

Arch Virol 2019 Feb 10. Epub 2019 Feb 10.

Instituto Pasteur, Secretaria da Saúde do Governo do estado de São Paulo, Avenida Paulista, 393, Bairro Cerqueira César, São Paulo, São Paulo, 01311-000, Brazil.

Bats play a significant role in maintaining their ecosystems through pollination, dispersal of seeds, and control of insect populations, but they are also known to host many microorganisms and have been described as natural reservoirs for viruses with zoonotic potential. The diversity of viruses in these animals remains largely unknown, however, because studies are limited by species, location, virus target, or sample type. Therefore, the aim of this study was to detect fragments of viral genomes in bat samples. Read More

Glob Vaccines Immunol 2018 Sep 15;3(1). Epub 2018 May 15.

Division of Infectious Diseases, Department of Immunology, Mayo Clinic, Rochester, MN, USA.

HIV-1 infections occur during sexual contact at mucosal surfaces. Vaccines need to provide mucosal barrier protection and stimulate systemic immune responses to control HIV spread. Most vaccines are delivered by systemic immunization via intramuscular (IM) injection route. Read More

Mol Ther Methods Clin Dev 2019 Jun 31;13:112-120. Epub 2018 Dec 31.

INSERM, U1016, Cochin Institute, Paris 75014, France.

Hematopoietic stem cell transplantation (HSCT) using unrelated cord blood (CB) donors is a suitable approach when an HLA-matched donor is not available. However, one important drawback is the risk of life-threatening viral infections prior to immune reconstitution, particularly from adenoviruses (AdVs). Although adoptive therapy with expanded virus-reactive donor T cells has proven effective to treat these infections in HSCT recipients, the manufacturing process is complex and requires large numbers of cells, which is incompatible with CB donor units. Read More

Therap Adv Gastroenterol 2019 22;12:1756284818821560. Epub 2019 Jan 22.

Department of Infectious Disease, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, China.

Liver cancer is one of the most common malignant tumors and prognosis remains poor. It has been increasingly recognized that liver cancer stem cells (LCSCs) are responsible for the carcinogenesis, recurrence, metastasis and chemoresistance of hepatocellular carcinoma (HCC). Targeting LCSCs is promising to be a new direction for the treatment of HCC. Read More

Vet Immunol Immunopathol 2019 Feb 18;208:44-52. Epub 2018 Dec 18.

College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi Province, 712100, PR China. Electronic address:

Melanoma Differentiation-Associated protein 5 (MDA5) is a cytoplasmic sensor for viral invasion and plays an important role in regulation of the immune response against Newcastle disease virus (NDV) in chickens. MDA5 was used as an adjuvant to enhance the humoral immune response against influenza virus. In the current study, truncated chicken MDA5 [1-483 aa, chMDA5(483aa)] expressed by recombinant adenovirus was administered to specific-pathogen-free (SPF) chickens to improve the immune response induced by inactivated NDV vaccine. Read More

Virus Res 2019 Jan 31;263:164-168. Epub 2019 Jan 31.

Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.

A 15-year-old female cockatiel (Nymphicus hollandicus) undergoing long term management for hepatopathy died and underwent necropsy. Microscopic findings were consistent with chronic liver disease characterized by distorted hepatic architecture, fibrosis and biliary proliferation. The additional finding of large intranuclear inclusion bodies within hepatocytes and renal tubular epithelium prompted diagnostic next generation sequencing. Read More

Int J Mol Sci 2019 Jan 31;20(3). Epub 2019 Jan 31.

Targovax ASA, Clinical Science, 0283 Oslo, Norway.

Oncolytic adenoviruses can trigger lysis of tumor cells, induce an antitumor immune response, bypass classical chemotherapeutic resistance strategies of tumors, and provide opportunities for combination strategies. A major challenge is the development of scalable production methods for viral seed stocks and sufficient quantities of clinical grade viruses. Because of promising clinical signals in a compassionate use program (Advanced Therapy Access Program) which supported further development, we chose the oncolytic adenovirus ONCOS-401 as a testbed for a new approach to scale up. Read More

Viruses 2019 Jan 31;11(2). Epub 2019 Jan 31.

Bioinformatics and Computational Biology Program, School of Systems Biology, George Mason University, Manassas, VA 20110, USA.

Human adenovirus type 4 (HAdV-E4), which is intriguingly limited to military populations, causes acute respiratory disease with demonstrated morbidity and mortality implications. This respiratory pathogen contains genome identity with chimpanzee adenoviruses, indicating zoonotic origins. A signature of these "old" HAdV-E4 is the absence of a critical replication motif, NF-I, which is found in all HAdV respiratory pathogens and most HAdVs. Read More

Viruses 2019 Jan 30;11(2). Epub 2019 Jan 30.

Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea.

Pathologies induced by viral infections have undergone extensive study, with traditional model systems such as two-dimensional (2D) cell cultures and in vivo mouse models contributing greatly to our understanding of host-virus interactions. However, the technical limitations inherent in these systems have constrained efforts to more fully understand such interactions, leading to a search for alternative in vitro systems that accurately recreate in vivo physiology in order to advance the study of viral pathogenesis. Over the last decade, there have been significant technological advances that have allowed researchers to more accurately model the host environment when modeling viral pathogenesis in vitro, including induced pluripotent stem cells (iPSCs), adult stem-cell-derived organoid culture systems and CRISPR/Cas9-mediated genome editing. Read More

Poult Sci 2019 Jan;98(1):97-104

Department of Virology, Faculty of Veterinary Medicine, Cairo University, Cairo 11361, Egypt.

This study was performed to isolate fowl adenovirus (FAdV) circulating in commercial meat-type chicken in Egypt during 2015 and to identify the pathogenicity of the isolated virus. Cloacal swabs were collected from 9 commercial broiler farms from chickens of 3-5 wk of age in Behira province in Egypt during 2015. FAdV was isolated on chicken embryo liver cells. Read More

Poult Sci 2019 Jan;98(1):404-412

College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.

Egg drop syndrome virus (EDSV), a member of the family Adenoviridae and an economically important pathogen with a broad host range, leads to markedly decreased egg production. However, the molecular mechanism underlying the host-EDSV interaction remains unclear. Here, we performed high-throughput RNA sequencing (RNA-Seq) to study the dynamic changes in host gene expression at 6, 12, and 24 hours post-infection in duck embryo fibroblasts (DEFs) infected with EDSV. Read More

Mil Med 2019 Jan 23. Epub 2019 Jan 23.

US Army Public Health Center, Aberdeen Proving Ground, MD.

Introduction: Chlamydia pneumoniae (Cp) is a bacterium that causes pneumonia and other respiratory diseases. Fever may be present early but absent by time of presentation to clinic. Increases in X-ray-confirmed pneumonia (XCP) and laboratory-confirmed Cp infections were observed in new soldiers in training at Fort Leonard Wood (FLW), Missouri, early in 2014. Read More

Sci Rep 2019 Jan 24;9(1):573. Epub 2019 Jan 24.

Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo, Japan.

Recently, bat adenoviruses (BtAdVs) of genus Mastadenovirus have been isolated from various bat species, some of them displaying a wide host range in cell culture. In this study, we isolated two BtAdVs from Japanese wild microbats. While one isolate was classified as Bat mastadenovirus A, the other was phylogenetically independent of other BtAdVs. Read More

Biomedicines 2019 Jan 22;7(1). Epub 2019 Jan 22.

Virginia Commonwealth University School of Medicine, Richmond, VA 23103, USA.

In their comprehensive review on adenoviruses, Khanal et al. omitted obesity as a disease caused by adenovirus 36 (Adv36). Animal studies have shown that experimental infection with Adv36 causes increased adiposity, and human association studies have shown that prior infection with Adv36 is correlated with greater body weight in humans in multiple countries of the world. Read More

Neuroscience 2019 Jan 21;402:11-22. Epub 2019 Jan 21.

Department of Demyelinating Disease and Aging, National Institute of Neuroscience, NCNP, Kodaira, Tokyo 187-8502, Japan. Electronic address:

Abnormalities of the autophagy-lysosomal pathway (ALP) have been implicated in the pathology of Alzheimer's disease (AD). Activation of TFEB (transcription factor EB), a master regulator of the ALP, leads to ALP facilitation. The present study sought to clarify whether TFEB-mediated ALP facilitation influences the process of amyloid β-protein (Aβ) generation in neurons. Read More

Sci Transl Med 2019 Jan;11(476)

Institut de Recerca Sant Joan de Deu, Barcelona 08950, Spain.

Retinoblastoma is a pediatric solid tumor of the retina activated upon homozygous inactivation of the tumor suppressor VCN-01 is an oncolytic adenovirus designed to replicate selectively in tumor cells with high abundance of free E2F-1, a consequence of a dysfunctional RB1 pathway. Thus, we reasoned that VCN-01 could provide targeted therapeutic activity against even chemoresistant retinoblastoma. In vitro, VCN-01 effectively killed patient-derived retinoblastoma models. Read More

mSphere 2019 01 23;4(1). Epub 2019 Jan 23.

Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Viral Metagenomics, KU Leuven-University of Leuven, Leuven, Belgium

Diarrhea remains one of the most common causes of deaths in children. A limited number of studies have investigated the prevalence of enteric pathogens in Cameroon, and as in many other African countries, the cause of many diarrheal episodes remains unexplained. A proportion of these unknown cases of diarrhea are likely caused by yet-unidentified viral agents, some of which could be the result of (recent) interspecies transmission from animal reservoirs, like bats. Read More

BMC Bioinformatics 2019 Jan 23;20(1):48. Epub 2019 Jan 23.

Department of Laboratory Medicine, University of Washington, Seattle, WA, USA.

Background: With sequencing technologies becoming cheaper and easier to use, more groups are able to obtain whole genome sequences of viruses of public health and scientific importance. Submission of genomic data to NCBI GenBank is a requirement prior to publication and plays a critical role in making scientific data publicly available. GenBank currently has automatic prokaryotic and eukaryotic genome annotation pipelines but has no viral annotation pipeline beyond influenza virus. Read More

Food Environ Virol 2019 Mar 23;11(1):32-39. Epub 2019 Jan 23.

Department of Soil, Water and Environmental Science, Water & Energy Sustainable Technology (WEST) Center, The University of Arizona, 2959 W. Calle Agua Nueva, Tucson, AZ, 85745, USA.

The buffalo green monkey (BGM) cell line is required for the detection of enteric viruses in biosolids through a total culturable viral assay (TCVA) by the United States Environmental Protection Agency. In the present study, BGM and PLC/PRF/5 cell lines were evaluated for TCVA and for their use in determining the incidence of adenoviruses and enteroviruses in raw sludge and Class B biosolids. Six raw sludge and 17 Class B biosolid samples were collected from 13 wastewater treatment plants from seven U. Read More

Hum Gene Ther 2019 Jan 23. Epub 2019 Jan 23.

University of Helsinki, Cancer Gene Therapy Group, Faculty of Medicine, Helsinki, Finland.

After discovery and characterization of the adenovirus in the 1950s, this prevalent cause of the common cold and other usually mild diseases has been modified and utilized in biomedicine in several ways. To date, adenoviruses are the most frequently used vectors and therapeutic (e.g. Read More

Vaccine 2019 Feb 19;37(8):1118-1125. Epub 2019 Jan 19.

Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonoses, College of Veterinary Medicine, Jilin University, Changchun, Jilin 130062, China; Key Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang, Liaoning 110866, China. Electronic address:

Toxoplasmosis is a worldwide zoonosis caused by the protozoan parasite Toxoplasma gondii, an obligate intracellular parasite. Currently, no viable vaccine or effective drug strategies exist to prevent and control toxoplasmosis. T. Read More

J Med Virol 2019 Jan 19. Epub 2019 Jan 19.

Department of Ophthalmology, Fukushima Medical University School of Medicine, Hobara Eye Clinic, Date, Japan.

Objective: To compare and evaluate the sensitivity of a newly developed silver-amplified immunochromatography (SAI) kit with various immunochromatography (IC) kits for adenoviruses based on the detection limit (copies/test).

Methods: An SAI kit and four ophthalmic IC kits were evaluated. The detection limits of the five kits were determined using the limiting dilution method for 15 conjunctivitis-associated adenoviruses (adenoviruses 1, 2, 3, 4, 5, 7, 8, 11, 37, 53, 54, 56, 64, 81, and 85). Read More

Curr Opin Virol 2019 Jan 14;34:63-69. Epub 2019 Jan 14.

Wadsworth Center, New York State Department of Health, 120 New Scotland Avenue, Albany, NY 12208, USA. Electronic address:

Human adenoviruses (HAdVs) are prevalent causes of acute respiratory disease (ARD) in military and civilian communities. Over the last 20 years, collaborative efforts between US public health, military and academic laboratories have gathered comprehensive data documenting the emergence and re-emergence of specific HAdV types in association with outbreaks and unrelated cases of ARD, which have attracted national attention. New or reemerging HAdVs have included genomic variants of HAdV-B14, HAdV-B7, and HAdV-E4. Read More

J Clin Virol 2019 Jan 11;112:1-9. Epub 2019 Jan 11.

Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan; Department of Ophthalmology, Health Sciences University of Hokkaido, Sapporo, Japan. Electronic address:

Adenoviral epidemic keratoconjunctivitis (EKC) presents as severe conjunctival inflammations involving the cornea that can lead to the development of corneal opacities and blurred vision, which can persist for months. EKC is highly contagious and responsible for outbreaks worldwide, therefore accurate diagnosis and rapid containment are imperative. EKC is caused by a number of types within Human adenovirus species D (HAdV-D): 8, 37 and 64 (formerly known as 19a) and these types were considered the major causes of EKC for over fifty years. Read More

Autophagy 2019 Jan 17:1-28. Epub 2019 Jan 17.

b School of Pharmaceutical Science , Wenzhou Medical University , Wenzhou , P.R. China.

Studies regarding macroautophagic/autophagic regulation in endothelial cells (ECs) under diabetic conditions are very limited. Clinical evidence establishes an endothelial protective effect of metformin, but the underlying mechanisms remain unclear. We aimed to investigate whether metformin exerts its protective role against hyperglycemia-induced endothelial impairment through the autophagy machinery. Read More

Med Pr 2018 Dec 18. Epub 2018 Dec 18.

Centralny Instytut Ochrony Pracy - Państwowy Instytut Badawczy / Central Institute for Labor Protection - National Research Institute, Warszawa, Poland (Zakład Zagrożeń Chemicznych, Pyłowych i Biologicznych, Pracownia Zagrożeń Biologicznych / Department of Chemical, Biological and Aerosol Hazards, Laboratory of Biohazards).

Solid waste storage in specially designated areas - landfills - has been the basic method of municipal solid waste disposal management for many years. However, thermal treatment is the currently preferred method of solid waste disposal. Hygienic assessment of working conditions in municipal waste incineration plants is hindered due to the variability and complexity of pollutants emitted during the combustion process. Read More

Healthcare (Basel) 2019 Jan 13;7(1). Epub 2019 Jan 13.

Interdisciplinary Center for River Basin Environment, University of Yamanashi, Yamanashi 400-8511, Japan.

Enteric viruses are highly contagious and a major cause of waterborne gastroenteritis in children younger than five years of age in developing world. This study examined the prevalence of enteric virus infection in children with gastroenteritis to identify risk factors for co-infections. In total, 107 stool samples were collected from patients with acute gastroenteritis along with samples of their household drinking water and other possible contamination sources, such as food and hand. Read More

Virus Res 2019 Jan 10;263:47-54. Epub 2019 Jan 10.

Department of Population Health Sciences, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA, 24061, United States.

Turkey adenovirus 3 (TAdV-3) belongs to the genus Siadenovirus, family Adenoviridae. Previously, nucleotide sequencing and annotation of the Virginia avirulent strain (VAS) of TAdV-3 genome, isolated in our laboratory, indicated the presence of a total of 23 genes and open reading frames (ORFs). The goals of this study were 1) to delineate the growth kinetics of the virus using a qPCR-based infectivity assay, and 2) to determine the virus gene expression profile during the early and late phases of infection in target B lymphocytes. Read More

Braz J Microbiol 2019 Jan 5;50(1):117-125. Epub 2018 Dec 5.

Medical University, University Hospital Alexandrovska, Pediatric Clinic, 1 St. Georgi Sofiiski, 1431, Sofia, Bulgaria.

Acute lower respiratory infections (ALRIs) are a leading cause of morbidity and hospital admissions in children. This study aimed to determine the viral etiology of these infections in children aged < 5 years during three successive epidemic seasons in Bulgaria. Nasopharyngeal and throat specimens were collected from children with bronchiolitis and pneumonia during the 2015/2016, 2016/2017, and 2017/2018 seasons. Read More

Adv Virus Res 2019 10;103:167-202. Epub 2018 Nov 10.

National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, United States.

The Nucleocytoplasmic Large DNA Viruses (NCLDV) of eukaryotes (proposed order "Megavirales") comprise an expansive group of eukaryotic viruses that consists of the families Poxviridae, Asfarviridae, Iridoviridae, Ascoviridae, Phycodnaviridae, Marseilleviridae, Pithoviridae, and Mimiviridae, as well as Pandoraviruses, Molliviruses, and Faustoviruses that so far remain unaccounted by the official virus taxonomy. All these viruses have double-stranded DNA genomes that range in size from about 100 kilobases (kb) to more than 2.5 megabases. Read More

J Transl Med 2019 Jan 11;17(1):19. Epub 2019 Jan 11.

Department of Radiology, University of Mississippi Medical Center, Jackson, MS, 39126, USA.

Background: Gene transfer to malignant sites using human adenoviruses (hAds) has been limited because of their immunogenic nature and host specificity. Murine cells often lack some of the receptors needed for hAds attachment, thus murine cells are generally non-permissive for human adenoviral infection and replication, which limits translational studies.

Methods: We have developed a gene transfer method that uses a combination of lipid-encapsulated perfluorocarbon microbubbles and ultrasound to protect and deliver hAds to a target tissue, bypassing the requirement of specific receptors. Read More

BMC Infect Dis 2019 Jan 9;19(1):36. Epub 2019 Jan 9.

Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China.

Background: Human adenovirus type 3 (HAdV-3) and 7 (HAdV-7) cause significant morbidity and develop severe complications and long-term pulmonary sequelae in children. However, epidemiologic reports have suggested that nearly all highly severe or fatal adenoviral diseases in children are associated with HAdV-7 rather than HAdV-3. Here, we conduct in-depth investigations to confirm and extend these findings through a comprehensive series of assays in vitro and in vivo as well as clinical correlates. Read More

Int J Mol Sci 2019 Jan 8;20(1). Epub 2019 Jan 8.

CHU de Québec⁻Université Laval Research Center, Quebec, QC G1R 2J6, Canada.

Localized prostate cancer (PCa) is often curable, whereas metastatic disease treated by castration inevitably progresses toward castration-resistant PCa (CRPC). Most CRPC treatments target androgen receptor (AR) signaling. However, not all CRPC cells rely on AR activity for survival and proliferation. Read More

Kidney Res Clin Pract 2018 Dec 31;37(4):414-417. Epub 2018 Dec 31.

Division of Nephrology, Department of Pediatrics, Seoul National University College of Medicine, Seoul, Korea.

Disseminated adenovirus infection can result in high mortality and morbidity in immunocompromised patients. Here, we report the case of a 10-year-old renal allograft recipient who presented with hematuria and dysuria. Adenovirus was isolated from his urine. Read More

Front Microbiol 2018 10;9:3040. Epub 2018 Dec 10.

State Key Laboratories of Respiratory Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.

Human adenoviruses type 4 (HAdV4) and 7 (HAdV7) are two major respiratory pathogens and sporadically cause outbreaks of acute respiratory diseases. The neutralizing antibody (nAb) response to these two adenoviruses in civilian populations, which is important for dissecting previous circulations and predicting potential outbreaks, remains largely unknown. In this study, we generated replication-competent HAdV4 and HAdV7 reporter viruses expressing secreted-alkaline-phosphatase (SEAP), and established neutralization assays to investigate the seroprevalence of pre-existing nAb in healthy volunteers from Hunan Province, southern China. Read More

BMC Ophthalmol 2019 Jan 7;19(1). Epub 2019 Jan 7.

Department of Ophthalmology, Faculty of Medicine, Tottori University, 36-1 Nishicho Yonago City, Tottori, Japan.

Background: Of the 10 patients with adenoviral type 54 keratoconjunctivitis examined at Nojima Hospital, 2 developed stellate keratitis and mutton-fat keratic precipitates (KPs) following acute symptoms.

Case Presentation: We encountered 10 cases of epidemic keratoconjunctivitis from August to October 2017. All patients were adults with a mean age of 60. Read More

Oncotarget 2018 Nov 30;9(94):36693-36704. Epub 2018 Nov 30.

Department of Pharmacology, University of Minnesota, Minneapolis 55455, MN, USA.

The metabolic protein alpha-methylacyl-CoA racemase (AMACR) is significantly overexpressed in prostate cancer compared to the normal prostate and other non-malignant tissue. Though an attractive target, there are no reports in the literature on leveraging the expression of AMACR for the molecular imaging of prostate cancer. Here, we used a molecular-genetic imaging strategy to exploit the transcriptional specificity of the AMACR promoter for the detection of prostate cancer using the reporter gene luciferase. Read More

Trop Anim Health Prod 2019 Jan 5. Epub 2019 Jan 5.

Department of Poultry Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.

A case-control study was performed to assess prescence of inclusion body hepatitis (IBH) caused by fowl adenoviruses (FAdVs) at Kafr EL-Shiekh Governorate, Egypt, during spring, 2017. The case group consisted of 100 liver and spleen samples collected from 10 broiler chickens flocks (10 samples from each flock) suspected to be infected with IBH depending on clinical manefestations and necropsy examination. Controls were randamly selected from chickens without clinical sings or evidence of the disease on postmortem examination. Read More

Ann Rheum Dis 2019 Mar 4;78(3):421-428. Epub 2019 Jan 4.

National Creative Research Initiatives Center for Osteoarthritis Pathogenesis and School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea

Objective: Osteoarthritis (OA) appears to be associated with various metabolic disorders, but the potential contribution of amino acid metabolism to OA pathogenesis has not been clearly elucidated. Here, we explored whether alterations in the amino acid metabolism of chondrocytes could regulate OA pathogenesis.

Methods: Expression profiles of amino acid metabolism-regulating genes in primary-culture passage 0 mouse chondrocytes were examined by microarray analysis, and selected genes were further characterised in mouse OA chondrocytes and OA cartilage of human and mouse models. Read More

IUBMB Life 2018 Dec 30. Epub 2018 Dec 30.

Department of Cardiology, First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.

Vascular smooth muscle cell (VSMC) hyperproliferation is the main pathological process in various cardiovascular diseases, such as vascular restenosis. This process may be repressed by RING finger protein 10 (RNF10) in metabolic syndrome (MetS) rats. The aim of this study is to evaluate the inhibitory effects and molecular mechanisms of RNF10 on VSMC hyperproliferation. Read More