Diagn Microbiol Infect Dis 2021 Apr 22;101(1):115401. Epub 2021 Apr 22.
Department of Orthopedic Surgery, Chinese PLA General Hospital, Beijing, P. R. China; Department of Orthopedic Surgery, Fourth center of Chinese PLA General Hospital, Beijing, P. R. China. Electronic address:
Three human adenovirus (HAdV) genotypes, HAdV-7, HAdV-14, and HAdV-55, emerged as the most prevalent variants in China over the past decade and caused both sporadic, fatal cases and frequent, large outbreaks. Early diagnosis is essential to control infections and endemics. Here, we established a loop-mediated isothermal amplification (LAMP) assay coupled with an instrument-free nucleic acid extraction device recently developed by our group; the assay could detect all the 3 prevalent HAdV genotypes. Read More