7,919 results match your criteria Acta cytologica[Journal]


Development and Validation of Molecular Assays for Limited Tissue Samples.

Acta Cytol 2019 Apr 17:1-8. Epub 2019 Apr 17.

Center for Personalized Diagnostics, Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA,

As the value of molecular testing of cancer specimens increases, the number of tests imposed on tumor specimens also increases, often in tension with the amount of tumor material available. To develop and validate molecular assays for limited specimens, there are specific concerns that must be addressed, including DNA quality, quantity, and abundance; the number of targets/ability to multiplex; and the analytical sensitivity and specificity of the assay itself. Ultimately, weighing these considerations during assay validation in the overall context of clinical utility and laboratory workflow is critical for delivering the highest level of personalized care to patients. Read More

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https://www.karger.com/Article/FullText/499109
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http://dx.doi.org/10.1159/000499109DOI Listing
April 2019
1 Read

Accuracy of Intraoperative Examination in Central Nervous System Lesions: A Study of 133 Cases.

Acta Cytol 2019 Apr 12:1-10. Epub 2019 Apr 12.

Department of Medicine, Universidade da Região de Joinville - UNIVILLE, Joinville, Brazil.

Objective: Intraoperative examination is a highly valuable tool for the evaluation of central nervous system (CNS) lesions, helping the neurosurgeon to determine the best surgical management. This study aimed to evaluate the accuracy and to analyze the diagnostic disagreements and pitfalls of the intraoperative examinations through correlation with the final histopathological diagnosis in CNS lesions.

Study Design: Retrospective analysis of intraoperative examination of CNS lesions and their final diagnosis obtained during 16 consecutive years. Read More

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http://dx.doi.org/10.1159/000495175DOI Listing

Role of Ancillary Techniques in Cervical Biopsy and Endocervical Curettage Specimens as Follow-Up to Papanicolaou Test Results Indicating a Diagnosis of Atypical Squamous Cells, Cannot Exclude High-Grade Squamous Intraepithelial Lesion, or High-Grade Squamous Intraepithelial Lesion.

Acta Cytol 2019 Apr 12:1-12. Epub 2019 Apr 12.

Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania, USA.

The Papanicolaou (PAP) test is widely used to screen for cervical cancer. All high-grade lesions such as atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion (ASC-H), and high-grade squamous intraepithelial lesion, identified on a PAP test should be followed-up by a confirmatory cervical biopsy. In this review, we discuss the challenges in interpreting cervical tissue specimens and the various ancillary techniques used in the evaluation of cervical dysplasia. Read More

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http://dx.doi.org/10.1159/000498888DOI Listing

Ancillary Techniques in Cytologic Specimens Obtained from Solid Lesions of the Pancreas: A Review.

Acta Cytol 2019 Apr 10:1-21. Epub 2019 Apr 10.

Division of Cytopathology, Department of Pathology and Laboratory Medicine, New York-Presbyterian Hospital-Weill Cornell Medical College, New York, New York, USA.

Advanced methods of molecular characterization have elucidated the genetic, epigenetic, and proteomic alterations associated with the broad spectrum of pancreatic disease, particularly neoplasia. Next-generation sequencing, in particular, has revealed the genomic diversity among pancreatic ductal adenocarcinoma, neuroendocrine and acinar tumors, solid pseudopapillary neoplasm, and other pancreatico-biliary neoplasms. Differentiating these entities from one another by morphologic analysis alone may be challenging, especially when examining the small quantities of diagnostic material inherent to cytologic specimens. Read More

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http://dx.doi.org/10.1159/000497153DOI Listing
April 2019
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The Positive Predictive Value of High-Grade Squamous Intraepithelial Lesion on Cytology for the Histological Diagnosis of Cervical Intraepithelial Neoplasia 2 or Higher: A Systematic Review.

Acta Cytol 2019 Apr 4:1-9. Epub 2019 Apr 4.

AMBIOR, Laboratory of Cell Biology and Histology, University of Antwerp, Antwerp, Belgium.

Cervical cancer is a major worldwide health problem. Therefore, regular cervical screening in order to make an early diagnosis can help to prevent cervical cancer, through identifying and treating preinvasive cervical lesions. The aim of this review is to evaluate the correlation between the cytological screening result and the final gold standard histological outcome in the diagnosis of cervical lesions. Read More

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http://dx.doi.org/10.1159/000497110DOI Listing
April 2019
1 Read

Role of Ancillary Techniques in Thyroid Cytology Specimens.

Acta Cytol 2019 Apr 4:1-12. Epub 2019 Apr 4.

Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.

Ancillary molecular testing has emerged as a promising way to refine the preoperative risk stratification of thyroid nodules with indeterminate fine needle aspiration (FNA) biopsy results. Commercially available molecular tests for thyroid FNAs include those that analyze samples for mutations and gene fusions, gene expression alterations, microRNA expression alterations, chromosomal copy number alterations, or a combination thereof. This review summarizes the performance characteristics of the most current iterations of three tests currently marketed for cytologically indeterminate thyroid nodules: ThyroSeq v3, ThyGeNEXT/ThyraMIR, and Afirma Gene Sequencing Classifier. Read More

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http://dx.doi.org/10.1159/000496502DOI Listing
April 2019
3 Reads

Role of Cytomorphology in the Era of Liquid Biopsy.

Acta Cytol 2019 Apr 3:1-9. Epub 2019 Apr 3.

Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal.

In the late stages of non-small cell lung cancer, the detection of sensitizing mutations of the epidermal growth factor receptor (EGFR) is mandatory to select patients' treatment with first- or second-generation tyrosine kinase inhibitors (TKIs). In patients showing progressive disease, the assessment of the EGFR exon 20 resistance point mutation p.T790M is required for third-generation TKI administration. Read More

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http://dx.doi.org/10.1159/000499338DOI Listing
April 2019
5 Reads

Human Papillomavirus Research: Where Should We Place Our Bets?

Acta Cytol 2019 Mar 28;63(2):1-12. Epub 2019 Mar 28.

Wolfson Institute of Preventive Medicine, Centre for Cancer Prevention, Queen Mary University of London, Barts and the London School of Medicine, London, United Kingdom.

Background: Massive strides have been made with respect to primary and secondary prevention of human papillomavirus (HPV)-associated disease as a result of prophylactic vaccination and cervical screening based on molecular HPV testing. However, cervical cancer continues to be an important clinical and societal burden. Additionally, other HPV-associated cancers, for which there are no screening programmes, are rising. Read More

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https://www.karger.com/Article/FullText/493800
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http://dx.doi.org/10.1159/000493800DOI Listing
March 2019
5 Reads

CytoProcessorTM: A New Cervical Cancer Screening System for Remote Diagnosis.

Acta Cytol 2019 Mar 28:1-9. Epub 2019 Mar 28.

DATEXIM, Caen, France.

Background: Current automated cervical cytology screening systems still heavily depend on manipulation of glass slides. We developed a new system called CytoProcessorTM (DATEXIM, Caen, France), which increases sensitivity and takes advantage of virtual slide technology to simplify the workflow and save worker time. We used an approach based on artificial intelligence to identify abnormal cells among the tens of thousands in a cervical preparation. Read More

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http://dx.doi.org/10.1159/000497111DOI Listing

Overview of Molecular Testing of Cytology Specimens.

Acta Cytol 2019 Mar 27:1-11. Epub 2019 Mar 27.

Objective: Utilizing cytology specimens for molecular testing has attracted increasing attention in the era of personalized medicine. Cytology specimens are clinically easier to access. The samples can be quickly and completely fixed in a very short time of fixation before tissue degradation occurs, compared to hours or days of fixation in surgical pathology specimens. Read More

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https://www.karger.com/Article/FullText/497187
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http://dx.doi.org/10.1159/000497187DOI Listing
March 2019
2 Reads

Diagnosis of Lymphoma Using Fine-Needle Aspiration Biopsy and Core-Needle Biopsy: A Single-Institution Experience.

Acta Cytol 2019 Mar 25:1-8. Epub 2019 Mar 25.

Department of Pathology and Laboratory Medicine, Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Lake Success, New York, USA,

Objective: The objective is to study the efficacy of fine-needle aspiration biopsy (FNAB) and core-needle biopsy (CNB) in the diagnosis of lymphoma in a single institution.

Study Design: We retrospectively reviewed 635 FNAB/CNB cases performed in our institution to rule out lymphoma during a 4-year period and collected the relevant clinical and pathological information for statistical analysis.

Results And Conclusions: This cohort comprised 275 males and 360 females, with a median age of 57 years. Read More

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https://www.karger.com/Article/FullText/497252
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http://dx.doi.org/10.1159/000497252DOI Listing
March 2019
5 Reads

The Role of Ancillary Techniques in Salivary Gland Cytopathology Specimens.

Acta Cytol 2019 Mar 25:1-11. Epub 2019 Mar 25.

Department of Pathology, Emory University, Atlanta, Georgia, USA,

Salivary gland tumor aspiration cytology is a useful preoperative test to guide the most appropriate clinical and surgical management for these patients. Although salivary gland cytology is often useful to distinguish between non-neoplastic lesions, benign neoplasms and malignant neoplasms, there remain many challenges in this area. Specifically, these tumors are uncommon and may have considerable morphologic overlap, especially in the setting of a malignant tumor. Read More

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https://www.karger.com/Article/FullText/497109
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http://dx.doi.org/10.1159/000497109DOI Listing
March 2019
10 Reads

Diagnostic Value of Fine-Needle Aspiration in Male Breast Lesions.

Acta Cytol 2019 Mar 22:1-9. Epub 2019 Mar 22.

Department of Pathology, Massachusetts General Hospital, Boston, Massachusetts, USA,

Objective: Differentiation between gynecomastia, a common cause of male breast enlargement, and breast cancer is crucial for appropriate management. Fine-needle aspiration biopsy has been shown to be sensitive and specific in assessing female breast lesions, comparable to core needle biopsy. Few such studies have been conducted in men. Read More

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http://dx.doi.org/10.1159/000494486DOI Listing
March 2019
1 Read

Impact of Brush Cytology Analysis for the Diagnosis of Esophageal Squamous Cell Carcinoma: The Quality of Liquid-Based Preparation of Cytological Slides.

Acta Cytol 2019 Mar 21:1-7. Epub 2019 Mar 21.

Molecular Oncology Research Center, Barretos Cancer Hospital/Pio XII Foundation, Barretos, Brazil,

Objectives: The diagnostic performance of cytology in esophageal squamous cell carcinoma (ESCC) is meticulously described.

Methods: Cytological and biopsy specimens were prospectively taken during esophagogastroduodenoscopy of 123 individuals in 2013 and 2014. Cytology samples were maintained in preservative fluid until processing and biopsies were formalin-fixed and paraffin-embedded. Read More

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http://dx.doi.org/10.1159/000496567DOI Listing
March 2019
2 Reads

Retroperitoneal Tumor: A Silent Trespasser - Role of Image-Guided Fine-Needle Aspiration Cytology with Histopathological Correlation in Early Diagnosis.

Acta Cytol 2019 Mar 20:1-9. Epub 2019 Mar 20.

Department of Pathology and Lab Medicine, All India Institute of Medical Sciences (AIIMS), Raipur, India.

Objective: Retroperitoneal lesions present a great diagnostic challenge. Here we analyze the spectrum of retroperitoneal lesions and the utility of cytohistopathological correlation in early diagnosis.

Study Design: This 7-year study was undertaken in 338 patients with a retroperitoneal mass (kidney/adrenal/ pancreas/retroperitoneal lymph node, or soft tissue origin). Read More

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http://dx.doi.org/10.1159/000497077DOI Listing
March 2019
5 Reads
1.562 Impact Factor

Fine-Needle Aspiration Biopsy Management of the Axilla in Primary Breast Carcinoma.

Acta Cytol 2019 Mar 20:1-5. Epub 2019 Mar 20.

Pathology Department, St. Vincent's University Hospital, Dublin, Ireland.

Context: Fine-needle aspiration biopsy (FNAB) is frequently used to stage the axilla preoperatively in patients with primary breast carcinoma. In the light of the ACOSOG-Z0011 and AMAROS trials that specified sentinel lymph node biopsy as an inclusion criterion, the role of FNAB in axillary staging is changing.

Objective: This article will review the diagnostic accuracy of FNAB in staging of the axilla in patients with primary breast carcinoma. Read More

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https://www.karger.com/Article/FullText/496159
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http://dx.doi.org/10.1159/000496159DOI Listing
March 2019
3 Reads

Role of Ancillary Techniques in Gynecologic Cytopathology Specimens.

Acta Cytol 2019 Mar 19:1-8. Epub 2019 Mar 19.

Department of Pathology, University of Chicago Medicine, Chicago, Illinois, USA,

The association between high-risk genotypes of human papillomavirus (hr-HPV) and cervical cancer is well established. As hr-HPV testing is rapidly becoming a part of routine cervical cancer screening, either in conjunction with cytology or as primary testing, the management of hr-HPV-positive women has to be tailored in a way that increases the detection of cervical abnormalities while decreasing unnecessary colposcopic biopsies or other invasive procedures. In this review, we discuss the overall utility and strategies of hr-HPV testing, as well as the advantages and limitations of potential triage strategies for hr-HPV-positive women, including HPV genotyping, p16/Ki-67 dual staining, and methylation assays. Read More

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http://dx.doi.org/10.1159/000496569DOI Listing
March 2019
1 Read

An Insight into the Utility of Sub-Categorisation of Atypia of Undetermined Significance for Risk Stratification: A Retrospective Study on an Indian Cohort with Histopathological Correlation.

Acta Cytol 2019 Mar 19:1-7. Epub 2019 Mar 19.

Department of Pathology, Christian Medical College, Vellore, India.

Background: Atypia of undetermined significance or follicular lesion of undetermined significance (AUS/FLUS) criterion in thyroid fine-needle aspirates (FNAs) has been a heterogeneous entity with much inter-observer variation. Sub-categorisation of AUS/FLUS has been observed to play an effective role in risk stratification. We aimed to validate AUS/FLUS sub-categorisation in correlation with the spectrum of malignancy. Read More

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http://dx.doi.org/10.1159/000496600DOI Listing
March 2019
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A Practical Guide to the Role of Ancillary Techniques in the Diagnosis of Infectious Agents in Fine Needle Aspiration Samples.

Acta Cytol 2019 Mar 19:1-11. Epub 2019 Mar 19.

Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA,

Fine needle aspiration samples and small biopsies provide a minimally invasive diagnostic modality for mass lesions. When an infectious process is suspected based on initial evaluation, ancillary techniques can assist in making a specific diagnosis. Here we review the cytopathology that should prompt additional testing and review the availability and interpretation of special stains, immunohistochemistry, and in situ hybridization. Read More

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http://dx.doi.org/10.1159/000497076DOI Listing

Role of Immunocytochemistry in the Cytological Diagnosis of Pulmonary Tumors.

Acta Cytol 2019 Mar 15:1-14. Epub 2019 Mar 15.

Department of Oncology, Città della Salute e della Scienza, University of Turin, Turin, Italy,

Pulmonary cytology is a challenging diagnostic tool, and it is usually evaluated considering medical history and radiological findings in order to reach an accurate diagnosis. Since the majority of lung cancer patients have an advanced stage at diagnosis, a cytological specimen is frequently the only material available for diagnosis and further prognostic/predictive marker determination. Several types of specimens can be obtained from the respiratory system (including sputum, bronchoalveolar lavage, bronchial brushing, fine needle aspiration, and pleural fluid) with different technical preclinical management protocols and different diagnostic yields. Read More

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http://dx.doi.org/10.1159/000496030DOI Listing

HPV Vaccine: Updates and Highlights.

Acta Cytol 2019 Mar 14;63(2):159-168. Epub 2019 Mar 14.

Faculdade de Medicina, Universidade de São Paulo, Instituto do Câncer do Estado de São Paulo, São Paulo (SP), Brazil.

HPV is the most common sexually transmitted biological agent and is the cause of many conditions in men and women, including precancer lesions and cancer. Three prophylactic HPV vaccines targeting high-risk HPV types are available in many countries worldwide: 2-, 4- and 9-valent vaccines. All the 3 vaccines use recombinant DNA technology and are prepared from the purified L1 protein that self-assembles to form HPV type-specific empty shells. Read More

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https://www.karger.com/Article/FullText/497617
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http://dx.doi.org/10.1159/000497617DOI Listing
March 2019
19 Reads

HPV: A Virus-Dinosaur Living among Us?

Acta Cytol 2019 Mar 14;63(2):83-84. Epub 2019 Mar 14.

Laboratory of Medical Investigation 14, Faculty of Medicine, São Paulo University, São Paulo, Brazil,

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http://dx.doi.org/10.1159/000497814DOI Listing
March 2019
4 Reads

New Insights into the Role of Human Papillomavirus in Anal Cancer and Anal Wart Development.

Acta Cytol 2019 Mar 12;63(2):118-123. Epub 2019 Mar 12.

Faculty of Medicine of the University of Porto, Porto, Portugal,

Human papillomavirus is associated with several anogenital and oropharyngeal lesions, including warts, premalignant lesions, and cancer. There are specific groups that were identified as high-risk groups for anal squamous cell carcinoma and anal human papillomavirus infection, namely HIV-positive patients, men who have sex with men, women with genital tract neoplasia, and solid organ transplant recipients. Condylomas have classically been considered to be a benign lesion, with an exception made for the Buschke-Loewenstein tumor, but several publications have shown that a high percentage of condylomas harbor high-grade lesions. Read More

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http://dx.doi.org/10.1159/000491815DOI Listing
March 2019
6 Reads

HPV in Head and Neck Carcinomas: Different HPV Profiles in Oropharyngeal Carcinomas - Why?

Acta Cytol 2019 Mar 12;63(2):124-142. Epub 2019 Mar 12.

Department of Clinical Research, Biohit Oyj, Helsinki, Finland.

Background: The association of human papillomavirus (HPV) with head and neck squamous cell carcinoma (HNSCC) was first described in 1982-1983 by the authors of this review. Prompted by this discovery 35 years ago, an entirely new field of HPV research has emerged, resulting in a paradigm shift from smoking and alcohol as the only etiological factors to confirmation of HNSCC as an important group of HPV-related human malignancies.

Summary: In this review, the authors first describe the scope (i. Read More

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http://dx.doi.org/10.1159/000495727DOI Listing
March 2019
1 Read

Circulating Tumor DNA: A Step into the Future of Cancer Management.

Acta Cytol 2019 Mar 8:1-10. Epub 2019 Mar 8.

i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal,

Liquid biopsy was introduced to the oncology field with the promise of revolutionizing the management of cancer patients, minimizing the exposure to invasive procedures such as tissue biopsy, and providing reliable information regarding therapy response and detection of disease relapse. Despite the significant increase in the number of published studies on circulating tumor DNA (ctDNA) in the past years, the emphasis of most studies is on the development of new technologies or on the clinical utility of ctDNA. This leaves a clear gap of knowledge concerning the biology of ctDNA, such as the fundamental mechanisms through which DNA from tumor cells is released into the circulation. Read More

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https://www.karger.com/Article/FullText/492917
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http://dx.doi.org/10.1159/000492917DOI Listing
March 2019
7 Reads

BRAF Mutations in Lung Cancer.

Acta Cytol 2019 Mar 6:1-4. Epub 2019 Mar 6.

Department of Public Health, University of Naples Federico II, Naples, Italy,

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http://dx.doi.org/10.1159/000496478DOI Listing
March 2019
2 Reads

The Significance of Circulating Tumour Cells in the Clinic.

Acta Cytol 2019 Feb 28:1-13. Epub 2019 Feb 28.

Medical Devices Research Group, Department of Life Sciences, INL - International Iberian Nanotechnology Laboratory, Braga, Portugal,

Background: Despite the hype about circulating tumour cells (CTCs) in the early 2000s and their potential in the diagnosis of metastasis, in recent years, the hope for personalised cancer management relies more on circulating tumour (ct)DNA that has entered the clinic in a much more efficient way. So far, approved methods for CTCs in the clinic only provide the counting of CTCs, which enables monitoring of the progression of metastatic breast, prostate, and colorectal cancer patients with therapy. Approved methods for ctDNA facilitate the analysis of specific mutations in lung cancer, thereby providing indications for potentially successful treatments. Read More

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https://www.karger.com/Article/FullText/495417
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http://dx.doi.org/10.1159/000495417DOI Listing
February 2019
7 Reads

Human Papillomavirus and Genital Disease in Men: What We Have Learned from the HIM Study.

Acta Cytol 2019 Feb 22;63(2):109-117. Epub 2019 Feb 22.

Center for Translational Research in Oncology, Instituto do Cancer do Estado de Sao Paulo, Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, São Paulo, Brazil,

It is currently recognized that in addition to the major impact of human papillomavirus (HPV) infection in females, HPV causes considerable disease in men at the genitals, anal canal, and oropharynx. Specifically, genital HPV infections may progress to genital warts and penile carcinoma. Although studies concerning the natural history of HPV infections and associated neoplasias have mainly focused on women, during the last 2 decades considerable attention has been given in further understanding these infections in men. Read More

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http://dx.doi.org/10.1159/000493737DOI Listing
February 2019
4 Reads

HPV Infections in Heterosexual Couples: Mechanisms and Covariates of Virus Transmission.

Acta Cytol 2019 Feb 22;63(2):143-147. Epub 2019 Feb 22.

Department of Oral Pathology and Radiology, University of Turku, Turku, Finland,

Sexual intercourse is regarded as the primary route of human papillomavirus (HPV) transmission. Reported rates of the genotype-specific genital concordance of HPV infection among heterosexual partners vary. Most studies have evaluated only male/female genital transmission, but lately, the oral region has gained interest because of a rising trend of HPV-associated oropharyngeal cancer. Read More

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http://dx.doi.org/10.1159/000494710DOI Listing
February 2019
1 Read

Current Status of p16 Immunohistochemistry and HPV Testing in Fine Needle Aspiration Specimens of the Head and Neck.

Acta Cytol 2019 Feb 15:1-10. Epub 2019 Feb 15.

Department of Pathology, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania, USA,

Human papilloma virus (HPV)-related squamous cell carcinoma (SCC) is biologically unique and has a better prognosis than conventional SCC of the head and neck. p16 immunohistochemistry emerged as a valuable surrogate marker for HPV in oropharyngeal SCC. The criteria for a positive p16 result in tissue specimens are well established. Read More

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http://dx.doi.org/10.1159/000496158DOI Listing
February 2019

From HPV Infection to Lesion Progression: The Role of HLA Alleles and Host Immunity.

Acta Cytol 2019 Feb 15;63(2):148-158. Epub 2019 Feb 15.

Department of Oral Pathology, Institute of Dentistry, Faculty of Medicine, University of Turku, Turku, Finland.

Persistent high-risk human papillomavirus (HPV) infection has been associated with increased risk for cervical precancerous lesions and cancer. The host's genetic variability is known to play a role in the development of cervical cancer. The human leukocyte antigen (HLA) genes are highly polymorphic and have shown to be important risk determinants of HPV infection persistence and disease progression. Read More

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https://www.karger.com/Article/FullText/494985
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http://dx.doi.org/10.1159/000494985DOI Listing
February 2019
7 Reads

Breast Fine Needle Aspiration Biopsy Cytology Using the Newly Proposed IAC Yokohama System for Reporting Breast Cytopathology: The Experience of a Single Institution.

Acta Cytol 2019 Feb 15:1-6. Epub 2019 Feb 15.

Institute of Molecular Pathology and Immunology, University of Porto, Porto, Portugal,

Objective: Recently the International Academy of Cytology (IAC) proposed a new reporting system for breast fine needle aspiration biopsy (FNAB) cytology. We aimed to categorize our samples according to this classification and to assess the risk of malignancy (ROM) for each category as well as the diagnostic yield of breast FNAB.

Study Design: Breast FNAB specimens obtained between January 2007 and December 2017 were reclassified according to the newly proposed IAC Yokohama reporting system. Read More

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http://dx.doi.org/10.1159/000492638DOI Listing
February 2019
1 Read

Liquid Biopsy beyond Circulating Tumor Cells and Cell-Free DNA.

Acta Cytol 2019 Feb 15:1-10. Epub 2019 Feb 15.

Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal (i3S), Porto, Portugal,

Liquid biopsy represents the analysis of tumor-derived material in the blood and other body fluids of cancer patients. This portrays a minimally invasive detection tool for molecular biomarkers. Liquid biopsy has emerged as a complementary or alternative method to surgical biopsy. Read More

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http://dx.doi.org/10.1159/000493969DOI Listing
February 2019
3 Reads

Masood's and Modified Masood's Scoring Index: An Evaluation of Fine Needle Aspiration Cytology of Breast Lesions with Histopathological Correlation.

Acta Cytol 2019 Feb 13:1-7. Epub 2019 Feb 13.

Department of Pathology, ESIC Medical College, Faridabad, India.

Objective: As fine needle aspiration cytology (FNAC) is the primary tool for evaluation of breast lesions, it is essential to segregate lesions with low and high risk of malignancy. To address this, Masood proposed a cytological scoring system for categorization, Masood's Scoring Index (MSI), which was modified later (Modified Masood's Scoring Index [MMSI]). This study analyses the effectiveness of MMSI over MSI and assesses the concordance between cytological scoring and histopathology. Read More

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http://dx.doi.org/10.1159/000496139DOI Listing
February 2019

Circulating Tumour Cells in Predictive Molecular Pathology: Focus on Drug-Sensitive Assays and 3D Culture.

Acta Cytol 2019 Feb 13:1-11. Epub 2019 Feb 13.

Department of Public Health, University of Naples Federico II, Naples, Italy,

Molecular cytopathology is a rapidly evolving field of cytopathology that provides biological information about the response to personalised therapy and about the prognosis of neoplasms diagnosed on cytological samples. Biomarkers such as circulating tumour cells and circulating tumour DNA are increasingly being evaluated in blood and in other body fluids. Such liquid biopsies are non-invasive, repeatable, and feasible also in patients with severe comorbidities. Read More

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http://dx.doi.org/10.1159/000496213DOI Listing
February 2019
1 Read

A Study of Pap Smears in HIV-Positive and HIV-Negative Women from a Tertiary Care Center in South India.

Acta Cytol 2019 5;63(1):50-55. Epub 2019 Feb 5.

Department of Pathology, Kasturba Medical College, Manipal Academy of Higher Education, Mangalore, India.

Objectives: The aim of this work was to study the spectrum of epithelial abnormalities on Pap smears of HIV-positive women categorized as per the Bethesda System of Reporting Cervical Cytology, to correlate them with CD4 lymphocyte counts, and to compare them with the spectrum of abnormalities seen in a HIV-negative control group. Study Design and Methodology: The present study was a 6-year retrospective study conducted in the Department of Pathology at Kasturba Medical College, Mangalore, which included 150 Pap smears from HIV-positive and HIV-negative women, respectively. The Pap-stained slides of the cases were retrieved and studied. Read More

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http://dx.doi.org/10.1159/000496211DOI Listing
March 2019
14 Reads

Beta Human Papillomavirus and Associated Diseases.

Acta Cytol 2019 Jan 23;63(2):100-108. Epub 2019 Jan 23.

Infections and Cancer Biology Group, International Agency for Research on Cancer, World Health Organization, Lyon, France.

The cutaneous human papillomavirus (HPV), mostly from β- and γ-HPV genus, is ubiquitously distributed throughout the human body and may be part of the commensal flora. The association of β-HPVs and cutaneous squamous cell carcinoma (cSCC) development was initially reported in patients with the rare genetic disorder Epidermodysplasia verruciformis. Likewise, immunosuppressed organ transplant recipients have an increased susceptibility to β-HPV infections in the skin as well as to cSCC development. Read More

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https://www.karger.com/Article/FullText/492659
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http://dx.doi.org/10.1159/000492659DOI Listing
January 2019
4 Reads

Comparison of Conventional Cytology, Liquid-Based Cytology, and Cell Block in the Evaluation of Peritoneal Fluid in Gynecology Malignancies.

Acta Cytol 2019 9;63(1):63-72. Epub 2019 Jan 9.

Pathology Department, Shiraz University of Medical Sciences, Shiraz, Iran,

Background: Peritoneal washing cytology has become an accepted method in evaluating gynecology malignancies.

Objective: The aim of this work was to compare the conventional cytology, liquid-based cytology (LBC), and cell block in the evaluation of peritoneal fluid.

Materials And Methods: A total of 48 cases with ovarian and uterine malignancy were included in this study. Read More

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http://dx.doi.org/10.1159/000495571DOI Listing
March 2019
5 Reads

Manufactured Cell Blocks: Turning Smears into Sections.

Acta Cytol 2019 4;63(1):28-34. Epub 2019 Jan 4.

PathWest Laboratory Medicine, QE2 Medical Centre, Nedlands, Washington, Australia,

Whilst cytological smears are still the basis of cytodiagnosis, there is an increasing role for ancillary testing. Specimens obtained are not always optimal, often with limited material for ancillary studies. Several reports have described the utility of scraping material from cytological smears to manufacture cell blocks to provide material for ancillary studies. Read More

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http://dx.doi.org/10.1159/000493907DOI Listing
March 2019
9 Reads

Liquid Biopsy and Lung Cancer.

Acta Cytol 2018 Dec 19:1-8. Epub 2018 Dec 19.

Department of Public Health, University of Naples Federico II, Naples, Italy,

The identification of non-small cell lung cancer (NSCLC) patients potentially responsive to targeted therapies relies on a number of relevant biomarkers, including EGFR, ALK, ROS-1, and PD-L1. Biomarker identification is most commonly based on surgical sample collection. However, when tissues are difficult to reach or when multiple analyses are necessary to monitor tumor progression and treatment response, liquid biopsy is a valid noninvasive alternative. Read More

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https://www.karger.com/Article/FullText/492710
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http://dx.doi.org/10.1159/000492710DOI Listing
December 2018
15 Reads

Study of the Pre-Analytical Phase of an ISO 15189: 2012-Certified Cytopathology Laboratory: A 5-Year Institutional Experience.

Acta Cytol 2019 19;63(1):56-62. Epub 2018 Dec 19.

Department of Pathology, Himalayan Institute of Medical Sciences, Swami Rama Himalayan University, Dehradun, India.

Objective: Total quality management, which basically involves pre-analytical, analytical, and postanalytical phases, is relatively more difficult in cytopathology due to descriptive reports and subjective variability. The pre-analytical phase of total quality management constitutes a major burden of errors in the laboratory and it has not been widely studied in cytopathology except in cervical Pap smears. The present study was therefore conducted to study the pre-analytical phase over 5 years from April 2013 until May 2018 at an ISO 15189: 2012-certified cytopathology laboratory, in a resource-limited setting. Read More

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https://www.karger.com/Article/FullText/494567
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http://dx.doi.org/10.1159/000494567DOI Listing
March 2019
24 Reads

Adaptation of Alpha-Papillomavirus over Millennia.

Acta Cytol 2018 Dec 13;63(2):97-99. Epub 2018 Dec 13.

Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, Brazil,

Papillomaviruses (PVs) are a group of small DNA viruses that, with around 350 million years of evolution, acquired the capacity of infecting a broad range of vertebrates, including humans. To date, more than 300 PV types have been isolated. Viruses that have a long common evolutionary history with their host typically cause unapparent infections. Read More

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https://www.karger.com/Article/FullText/492658
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http://dx.doi.org/10.1159/000492658DOI Listing
December 2018
16 Reads

Subcutaneous Fat Necrosis of the Newborn: Is Fine Needle Aspiration Cytology Diagnostic?

Acta Cytol 2019;63(1):75-77. Epub 2018 Dec 13.

Department of Paediatric Surgery, Chacha Nehru Bal Chikitsalaya, Delhi, India.

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http://dx.doi.org/10.1159/000494501DOI Listing
March 2019
4 Reads

Correlation of Ultrasound Findings and Fine-Needle Aspiration Cytology for the Diagnosis of Axillary Lymph Node Metastasis in Patients with Breast Carcinoma.

Acta Cytol 2019 5;63(1):17-22. Epub 2018 Dec 5.

Department of Cytology, Kuwait Cancer Control Center, Safat, Kuwait.

Objective: Ultrasound-guided fine-needle aspiration cytology (USG-FNAC) is used for the detection of axillary lymph node (ALN) metastasis in patients with breast carcinoma (BC). US findings have a good diagnostic accuracy with high sensitivity and specificity. The aim of this study is to correlate the detection of ALN metastases on US with FNAC in BC patients. Read More

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https://www.karger.com/Article/FullText/493635
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http://dx.doi.org/10.1159/000493635DOI Listing
March 2019
8 Reads

Three-Tiered versus Two-Tiered Classification of Squamous Dysplasia in Cervical Cytology: Results of a Follow-Up Study.

Acta Cytol 2019 5;63(1):44-49. Epub 2018 Dec 5.

Institut für Biometrie und Klinische Epidemiologie, Charité - Universitätsmedizin Berlin, Berlin, Germany.

Objective: Regarding cytological findings of squamous dysplasia, a comparison was made between a three-tiered classification - low-grade squamous intraepithelial lesion (LSIL), high-grade SIL/cervical intraepithelial neoplasia 2 (HSIL/CIN2), and HSIL/CIN3 - and a two-tiered classification - LSIL and HSIL. The respective risk for CIN2+ and CIN3+ was calculated to make decisions regarding management.

Methods: A total of 2,949 women with first-time cytologic findings of squamous dysplasia (LSIL, HSIL/CIN2, or HSIL/CIN3) between January 2013 and June 2016 were enrolled. Read More

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http://dx.doi.org/10.1159/000494984DOI Listing
March 2019
3 Reads

Retinal Anlage Tumor of Epididymis: A Diagnostic Challenge on Cytology.

Acta Cytol 2019;63(1):73-74. Epub 2018 Nov 19.

Department of Paediatric Surgery, Chacha Nehru Bal Chikitsalaya, Delhi, India.

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http://dx.doi.org/10.1159/000494124DOI Listing
March 2019
3 Reads

BRAF V600 Mutational Status in Melanoma Correlates with Cytologic Features in Fine-Needle Aspirate Specimens.

Acta Cytol 2019 5;63(1):10-16. Epub 2018 Nov 5.

Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA.

Melanomas are known as the great mimicker and must be considered in the differential diagnosis of any fine-needle aspirations (FNA). Despite recent advancements in understanding of the mutational landscape of melanomas, there still exists a divide between the genetic and morphologic correlates. A consecutive cohort of 39 FNA of clinically verified metastatic melanomas with concurrent BRAF V600 assessment were selected [positive (n = 18) and wild-type (n = 21)]. Read More

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http://dx.doi.org/10.1159/000491814DOI Listing
March 2019
19 Reads

Diagnostic Accuracy of Fine-Needle Aspiration Cytology for Discrimination of Squamous Cell Carcinoma from Adenocarcinoma in Non-Small Cell Lung Cancer: A Systematic Review and Meta-Analysis.

Acta Cytol 2018 31;62(5-6):318-326. Epub 2018 Oct 31.

Department of Pathology and ARUP Laboratories, University of Utah, Salt Lake City, Utah, USA.

Objective: To determine the accuracy with which morphology alone can distinguish adenocarcinoma and squamous cell carcinoma in non-small cell lung cancer.

Methods: We performed a systematic review and meta-analysis. Three data bases (MEDLINE, EMBASE, Scopus) were searched for studies on the diagnostic accuracy of subtyping non-small cell lung cancer. Read More

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https://www.karger.com/Article/FullText/493942
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http://dx.doi.org/10.1159/000493942DOI Listing
January 2019
6 Reads

Cytomorphologic, Imaging, Molecular Findings, and Outcome in Thyroid Follicular Lesion of Undetermined Significance/Atypical Cell of Undetermined Significance (AUS/FLUS): A Mini-Review.

Acta Cytol 2019 31;63(1):1-9. Epub 2018 Oct 31.

Division of Cytopathology, Department of Pathology, The Johns Hopkins Hospital, Baltimore, Maryland,

Objectives: Since the introduction of the entity of "Atypical cell of undetermined significance /follicular lesion of undetermined significance" (AUS/FLUS) by The Bethesda System for Reporting Thyroid Cytology (TBSRTC) in 2007, there have been many published studies about the cytomorphologic criteria, subclassification, outcome, and management of patients with the diagnosis of AUS/FLUS. There have been many studies in different aspects of this indeterminate category, i.e. Read More

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https://www.karger.com/Article/FullText/493908
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http://dx.doi.org/10.1159/000493908DOI Listing
March 2019
15 Reads

The Differential Diagnosis in Nonlymphoproliferative Malignant Pleural Effusion Cytopathology and Its Correlation with Patients' Demographics.

Acta Cytol 2018 31;62(5-6):436-442. Epub 2018 Oct 31.

Division of Cytopathology, Department of Pathology, The Johns Hopkins Hospital, Baltimore, Maryland, USA.

Background: We report our experience with malignant pleural effusion (MPE) and the impact of patients' demographics on the differential diagnosis at the primary site.

Methods: After IRB approval, we searched our pathology database from January 2013 to January 2017 for patients with positive pleural effusions (PEs). Patients' demographics and clinical histories were noted. Read More

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http://dx.doi.org/10.1159/000493336DOI Listing
January 2019
10 Reads