17 results match your criteria Acta crystallographica. Section F Structural biology and crystallization communications[Journal]

  • Page 1 of 1

Preliminary crystallographic analysis of RraB from Escherichia coli.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2013 Nov 30;69(Pt 11):1268-71. Epub 2013 Oct 30.

School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, People's Republic of China.

RraB, an inhibitor of the essential endoribonuclease RNE in Escherichia coli, is essential in regulating the abundance of RNA by directly interacting with RNE. In this study, RraB from E. coli was cloned, expressed, purified and crystallized. Read More

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http://dx.doi.org/10.1107/S1744309113026559DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3818050PMC
November 2013
15 Reads

Acta Crystallographica Section F: Structural Biology Communications.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2013 Aug 30;69(Pt 8):827. Epub 2013 Jul 30.

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http://dx.doi.org/10.1107/S1744309113020873DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3729152PMC
August 2013
25 Reads

Crystallization reports are the backbone of Acta Cryst. F, but do they have any spine?

Acta Crystallogr Sect F Struct Biol Cryst Commun 2013 Jul 30;69(Pt 7):712-8. Epub 2013 Jun 30.

Materials, Science and Engineering Division, CSIRO, 343 Royal Parade, Parkville, VIC 3052, Australia.

Crystallization of macromolecules is famously difficult. By knowing what has worked for others, researchers can ease the process, both in the case where the protein has already been crystallized and in the situation where more general guidelines are needed. The 264 crystallization communications published in Acta Crystallographica Section F in 2012 have been reviewed, and from this analysis some information about trends in crystallization has been gleaned. Read More

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http://dx.doi.org/10.1107/S1744309113014152DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3702311PMC
July 2013
29 Reads

Review panel for Acta Crystallographica Section F.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2011 Nov 29;67(Pt 11):1309. Epub 2011 Oct 29.

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http://dx.doi.org/10.1107/S1744309111044277DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3212441PMC
November 2011
19 Reads

Structural genomics of infectious disease drug targets: the SSGCID.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2011 Sep 13;67(Pt 9):979-84. Epub 2011 Aug 13.

Seattle Structural Genomics Center for Infectious Disease, USA.

The Seattle Structural Genomics Center for Infectious Disease (SSGCID) is a consortium of researchers at Seattle BioMed, Emerald BioStructures, the University of Washington and Pacific Northwest National Laboratory that was established to apply structural genomics approaches to drug targets from infectious disease organisms. The SSGCID is currently funded over a five-year period by the National Institute of Allergy and Infectious Diseases (NIAID) to determine the three-dimensional structures of 400 proteins from a variety of Category A, B and C pathogens. Target selection engages the infectious disease research and drug-therapy communities to identify drug targets, essential enzymes, virulence factors and vaccine candidates of biomedical relevance to combat infectious diseases. Read More

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http://dx.doi.org/10.1107/S1744309111029204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169389PMC
September 2011
12 Reads

Purification, crystallization and preliminary X-ray crystallographic analysis of a central domain of human splicing factor 1.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2011 Apr 25;67(Pt 4):486-90. Epub 2011 Mar 25.

Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.

Pre-mRNA splicing is an essential source of genetic diversity in eukaryotic organisms. In the early stages of splicing, splicing factor 1 (SF1) recognizes the pre-mRNA splice site as a complex with its partner, U2 auxiliary factor 65 kDa subunit (U2AF(65)). A central `mystery' domain of SF1 (SF1md) lacks detectable homology with known structures, yet is the region of highest phylogenetic sequence conservation among SF1 homologues. Read More

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http://scripts.iucr.org/cgi-bin/paper?S1744309111004623
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http://dx.doi.org/10.1107/S1744309111004623DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3080157PMC
April 2011
15 Reads

TOPSAN: use of a collaborative environment for annotating, analyzing and disseminating data on JCSG and PSI structures.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2010 Oct 30;66(Pt 10):1143-7. Epub 2010 Sep 30.

Center for Research in Biological Systems, University of California, San Diego, La Jolla, California, USA.

The NIH Protein Structure Initiative centers, such as the Joint Center for Structural Genomics (JCSG), have developed highly efficient technological platforms that are capable of experimentally determining the three-dimensional structures of hundreds of proteins per year. However, the overwhelming majority of the almost 5000 protein structures determined by these centers have yet to be described in the peer-reviewed literature. In a high-throughput structural genomics environment, the process of structure determination occurs independently of any associated experimental characterization of function, which creates a challenge for the annotation and analysis of structures and the publication of these results. Read More

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http://scripts.iucr.org/cgi-bin/paper?S1744309110035736
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http://dx.doi.org/10.1107/S1744309110035736DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2954197PMC
October 2010
12 Reads

Laboratory communications in Acta Crystallographica Section F.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2009 Oct 30;65(Pt 10):962. Epub 2009 Sep 30.

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http://dx.doi.org/10.1107/S174430910903855XDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2765877PMC
October 2009
13 Reads

Structure of EstA esterase from psychrotrophic Pseudoalteromonas sp. 643A covalently inhibited by monoethylphosphonate.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2009 Sep 20;65(Pt 9):862-5. Epub 2009 Aug 20.

Synchrotron Radiation Research Section, MCL, National Cancer Institute, Argonne National Laboratory, Argonne, IL 60439, USA.

The crystal structure of the esterase EstA from the cold-adapted bacterium Pseudoalteromonas sp. 643A was determined in a covalently inhibited form at a resolution of 1.35 A. Read More

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http://dx.doi.org/10.1107/S1744309109030826DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2795586PMC
September 2009
30 Reads

Crystallization and preliminary X-ray diffraction of the DEAD-box protein Mss116p complexed with an RNA oligonucleotide and AMP-PNP.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2009 Aug 30;65(Pt 8):832-5. Epub 2009 Jul 30.

Institute for Cellular and Molecular Biology, Department of Chemistry and Biochemistry, and Section of Molecular Genetics and Microbiology, School of Biological Sciences, University of Texas at Austin, Austin, TX 78712, USA.

The Saccharomyces cerevisiae DEAD-box protein Mss116p is a general RNA chaperone which functions in mitochondrial group I and group II intron splicing, translation and RNA-end processing. For crystallization trials, full-length Mss116p and a C-terminally truncated protein (Mss116p/Delta598-664) were overproduced in Escherichia coli and purified to homogeneity. Mss116p exhibited low solubility in standard solutions (< or =1 mg ml(-1)), but its solubility could be increased by adding 50 mM L-arginine plus 50 mM L-glutamate and 50% glycerol to achieve concentrations of approximately 10 mg ml(-1). Read More

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http://dx.doi.org/10.1107/S1744309109027225DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720346PMC
August 2009
11 Reads

Crystallization and preliminary crystallographic analysis of the complex of the second and third regulatory subunits of human Pol delta.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2008 Sep 20;64(Pt 9):822-4. Epub 2008 Aug 20.

Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198-7696, USA.

Human DNA polymerase delta (Pol delta) consists of four subunits: p125, p50, p66 and p12. A heterodimer containing a His-tagged p50 subunit (p50) and a p50-interacting domain of the p66 subunit (p66(N)) was crystallized. The crystal was in the form of a prism with a rhombic cross-section and belonged to space group P2(1). Read More

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http://scripts.iucr.org/cgi-bin/paper?S1744309108025086
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http://dx.doi.org/10.1107/S1744309108025086DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2531283PMC
September 2008
10 Reads

Cloning, purification and preliminary X-ray analysis of the C-terminal domain of Helicobacter pylori MotB.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2008 Apr 21;64(Pt 4):277-80. Epub 2008 Mar 21.

Manchester Interdisciplinary Biocentre, Faculty of Life Sciences, University of Manchester, 131 Princess Street, Manchester M1 7DN, England.

The C-terminal domain of MotB (MotB-C) contains a putative peptidoglycan-binding motif and is believed to anchor the MotA/MotB stator unit of the bacterial flagellar motor to the cell wall. Crystals of Helicobacter pylori MotB-C (138 amino-acid residues) were obtained by the hanging-drop vapour-diffusion method using polyethylene glycol as a precipitant. These crystals belong to space group P2(1), with unit-cell parameters a = 50. Read More

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http://dx.doi.org/10.1107/S1744309108005277DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2374257PMC
April 2008
12 Reads

Expression, purification and preliminary X-ray characterization of DL-2-haloacid dehalogenase from Methylobacterium sp. CPA1.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2007 Jul 15;63(Pt 7):586-9. Epub 2007 Jun 15.

Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan.

DL-2-Haloacid dehalogenase from Methylobacterium sp. CPA1 (DL-DEX Mb) is a unique enzyme that catalyzes the dehalogenation reaction without the formation of an ester intermediate. A recombinant form of DL-DEX Mb has been expressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. Read More

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http://dx.doi.org/10.1107/S1744309107027273DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2335131PMC
July 2007
14 Reads

Cloning, expression, purification, crystallization and preliminary crystallographic analysis of pseudo death-effector domain of HIPPI, a molecular partner of Huntingtin-interacting protein HIP-1.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2006 Dec 30;62(Pt 12):1247-50. Epub 2006 Nov 30.

Structural Genomics Section, Saha Institute of Nuclear Physics, 1/AF Bidhan Nagar, Kolkata 700064, India.

The formation of a heterodimer between Huntingtin-interacting protein-1 (HIP-1) and its novel partner HIPPI (HIP-1 protein interactor) through their pseudo death-effector domains (pDEDs) is a key step that recruits caspase-8 and initiates apoptosis. This could be one of the pathways by which apoptosis is increased in Huntington's disease (HD). A construct consisting of the pDED of HIPPI has been cloned and overexpressed as 6NH-tagged protein and purified by Ni-NTA affinity chromatography. Read More

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http://dx.doi.org/10.1107/S1744309106046628DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225382PMC
December 2006
12 Reads

Expression, limited proteolysis and preliminary crystallographic analysis of IpaD, a component of the Shigella flexneri type III secretion system.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2006 Sep 11;62(Pt 9):865-8. Epub 2006 Aug 11.

Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, England.

IpaD, the putative needle-tip protein of the Shigella flexneri type III secretion system, has been overexpressed and purified. Crystals were grown of the native protein in space group P2(1)2(1)2(1), with unit-cell parameters a = 55.9, b = 100. Read More

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http://dx.doi.org/10.1107/S1744309106027047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1894744PMC
September 2006
18 Reads

Expression, purification, crystallization and preliminary crystallographic analysis of MxiH, a subunit of the Shigella flexneri type III secretion system needle.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2006 Mar 28;62(Pt 3):302-5. Epub 2006 Feb 28.

Department of Biochemistry, University of Oxford, England.

A monodisperse truncation mutant of MxiH, the subunit of the needle from the Shigella flexneri type III secretion system (TTSS), has been overexpressed and purified. Crystals were grown of native and selenomethionine-labelled MxiH(CDelta5) and diffraction data were collected to 1.9 A resolution. Read More

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http://dx.doi.org/10.1107/S1744309106006555DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1894743PMC
March 2006
14 Reads

Crystallization and preliminary X-ray analysis of methylthioribose-1-phosphate isomerase from Bacillus subtilis.

Acta Crystallogr Sect F Struct Biol Cryst Commun 2005 Jun 1;61(Pt 6):595-8. Epub 2005 Jun 1.

Department of Materials Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.

Methylthioribose-1-phosphate isomerase (MtnA) from Bacillus subtilis, the first enzyme in the downstream section of the methionine-salvage pathway, was crystallized using the sitting-drop vapour-diffusion method. Crystals grew using ammonium sulfate as the precipitant at 293 K. They diffracted to 2. Read More

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http://dx.doi.org/10.1107/S1744309105015757DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1952323PMC
June 2005
11 Reads
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