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    5218 results match your criteria Acta biomaterialia[Journal]

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    Macrophage involvement affects matrix stiffness-related influences on cell osteogenesis under three-dimensional culture conditions.
    Acta Biomater 2018 Feb 17. Epub 2018 Feb 17.
    State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases and Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Periodontology, School of Stomatology, Fourth Military Medical University, Xi'an, PR China. Electronic address:
    Accumulating evidence indicates that the physicochemical properties of biomaterials exert profound influences on stem cell fate decisions. However, matrix-based regulation selected through in vitro analyses based on a given cell population do not genuinely reflect the in vivo conditions, in which multiple cell types are involved and interact dynamically. This study constitutes the first investigation of how macrophages (Mφs) in stiffness-tunable transglutaminase cross-linked gelatin (TG-gel) affect the osteogenesis of bone marrow-derived mesenchymal stem cells (BMMSCs). Read More

    Exogenous mineralization of hard tissues using photo-absorptive minerals and femto-second lasers; the case of dental enamel.
    Acta Biomater 2018 Feb 17. Epub 2018 Feb 17.
    School of Chemical and Process Engineering, University of Leeds, Leeds LS2 9JT, UK.
    A radical new methodology for the exogenous mineralization of hard tissues is demonstrated in the context of laser-biomaterials interaction. The proposed approach is based on the use of femtosecond pulsed lasers (fs) and Fe-doped calcium phosphate minerals (specifically in this work fluorapatite powder containing FeOnanoparticles (NP)). A layer of the synthetic powder is applied to the surface of eroded bovine enamel and is irradiated with a fs laser (1040 nm wavelength, 1 GHz repetition rate, 150 fs pulse duration and 0. Read More

    Fibrin glue mesh fixation combined with mesenchymal stem cells or exosomes modulates the inflammatory reaction in a murine model of incisional hernia.
    Acta Biomater 2018 Feb 17. Epub 2018 Feb 17.
    Stem Cell Therapy Unit, 'Jesús Usón' Minimally Invasive Surgery Centre, Cáceres 10071, Spain; CIBER de Enfermedades Cardiovasculares, Spain; Immunology Unit, Department of Physiology, University of Extremadura, 10071 Caceres, Spain.. Electronic address:
    Surgical meshes are effective and frequently used to reinforce soft tissues. Fibrin glue (FG) has been widely used for mesh fixation and is also considered an optimal vehicle for stem cell delivery. The aim of this preclinical study was to evaluate the therapeutic effect of MSCs and their exosomes combined with FG for the treatment of incisional hernia. Read More

    Investigation of Adhesive Interactions in the Specific Targeting of Triptorelin-conjugated PEG-coated Magnetite Nanoparticles to Breast Cancer Cells.
    Acta Biomater 2018 Feb 16. Epub 2018 Feb 16.
    Department of Mechanical and Aerospace Engineering, Princeton University, Olden Street, Princeton, NJ 08544, USA; Princeton Institute of Science and Technology of Materials (PRISM), Princeton University, 70 Prospect Street, Princeton, NJ 08544, USA; Department of Mechanical Engineering, Higgins Lab, Worcester Polytechnic Institute (WPI), 100 Institute Road, Worcester, MA 01609, USA; Department of Biomedical Engineering, Gateway Park Life Sciences Center, Worcester Polytechnic Institute (WPI), 60 Prescott Street, Worcester, MA 01605, USA. Electronic address:
    The understanding of adhesive interaction at the nanoscale between functionalized nanoparticles and biological cells is of great importance to develop effective theranostic nanocarriers for targeted cancer therapy. Here, we report a combination of experimental and computational approaches to evaluate the adhesion between Triptorelin (a Luteinizing Hormone-Releasing Hormone (LHRH) agonist)-conjugated poly-(ethylene glycol) (PEG)-coated magnetite nanoparticles (Triptorelin-MNPs) and breast cells. The adhesion forces between Triptorelin-MNPs and normal/cancerous breast cells are obtained using atomic force microscopy. Read More

    An aligned porous electrospun fibrous membrane with controlled drug delivery- an efficient strategy to accelerate diabetic wound healing with improved angiogenesis.
    Acta Biomater 2018 Feb 14. Epub 2018 Feb 14.
    College of Life and Environmental Sciences, Shanghai Normal University, No.100 Guilin Road, Shanghai 200234, China. Electronic address:
    A chronic wound in diabetic patients is usually characterized by poor angiogenesis and delayed wound closure. The exploration of efficient strategy to significantly improve angiogenesis in the diabetic wound bed and thereby accelerate wound healing is still a significant challenge. Herein, we reported a kind of aligned porous poly (L-lactic acid) (PLLA) electrospun fibrous membranes containing dimethyloxalylglycine (DMOG)-loaded mesoporous silica nanoparticles (DS) for diabetic wound healing. Read More

    Polyisocyanopeptide hydrogels: a novel thermo-responsive hydrogel supporting pre-vascularization and the development of organotypic structures.
    Acta Biomater 2018 Feb 14. Epub 2018 Feb 14.
    Tissue Biology Research Unit, Department of Surgery, University Children's Hospital Zurich, August Forel Str. 7, CH-8008 Zurich, Switzerland. Electronic address:
    Molecular and mechanical interactions with the 3D extracellular matrix are essential for cell functions such as survival, proliferation, migration, and differentiation. Thermo-responsive biomimetic polyisocyanopeptide (PIC) hydrogels are promising new candidates for 3D cell, tissue, and organ cultures. This is a synthetic, thermo-responsive and stress-stiffening material synthesized via polymerization of the corresponding monomers using a nickel perchlorate as a catalyst. Read More

    Nanofibrous PLGA Electrospun Scaffolds Modified with Type I Collagen Influence Hepatocyte Function and Support Viability In Vitro.
    Acta Biomater 2018 Feb 14. Epub 2018 Feb 14.
    Comprehensive Transplant Center, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611, United States; Department of Surgery, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611, United States; Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois, 60208, United States; Department of Surgery, Jesse Brown VA Medical Center, Chicago, Illinois, 60612, United States; Simpson Querrey Institute for BioNanotechnology in Medicine, Northwestern University, Chicago, Illinois, 60611, United States; Department of Biomedical Engineering, Northwestern University, Evanston, Illinois 60208, United States. Electronic address:
    A major challenge of maintaining primary hepatocytes in vitro is progressive loss of hepatocyte-specific functions, such as protein synthesis and cytochrome P450 (CYP450) catalytic activity. We developed a three-dimensional (3D) nanofibrous scaffold made from poly(L-lactide-co-glycolide) (PLGA) polymer using a newlyoptimizedwet electrospinning techniquethat resulted in a highly porous structure that accommodated inclusion of primary human hepatocytes. Extracellular matrix (ECM) proteins (type I collagen or fibronectin) at varying concentrations were chemically linked to electrospun PLGA using amine coupling to develop an in vitro culture system containing the minimal essential ECM components of the liver micro-environment that preserve hepatocyte function in vitro. Read More

    A Laser-Activated Multifunctional Targeted Nanoagent for Imaging and Gene Therapy in a Mouse Xenograft Model with Retinoblastoma Y79 Cells.
    Acta Biomater 2018 Feb 13. Epub 2018 Feb 13.
    Department of Ophthalmology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, PR China. Electronic address:
    Retinoblastoma (RB) is the most common intraocular malignancy of childhood that urgently needs early detection and effective therapy methods. The use of nanosized gene delivery systems is appealing because of their highly adjustable structure to carry both therapeutic and imaging agents. Herein, we report a folic acid (FA)-modified phase-changeable cationic nanoparticle encapsulating liquid perfluoropentane (PFP) and indocyanine green (ICG) (FA-CN-PFP-ICG, FCNPI) with good plasmid DNA (pDNA) carrying capacity, favorable biocompatibility, excellent photoacoustic (PA) and ultrasound (US) contrast, enhanced gene transfection efficiency and therapeutic effect. Read More

    Control of Thiol-Maleimide Reaction Kinetics in PEG Hydrogel Networks.
    Acta Biomater 2018 Feb 13. Epub 2018 Feb 13.
    Department of Chemical Engineering, University of Massachusetts Amherst, 240 Thatcher Rd, Amherst, MA 01003-9364, United States. Electronic address:
    Michael-type addition reactions are widely used to polymerize biocompatible hydrogels. The thiol-maleimide modality achieves the highest macromer coupling efficiency of the reported Michael-type pairs, but the resulting hydrogel networks are heterogeneous, because polymerization is faster than the individual components can be manually mixed. The reactivity of the thiol dictates the overall reaction speed, which can be slowed in organic solvents and acidic buffers. Read More

    3D Bioprinted Functional and Contractile Cardiac Tissue Constructs.
    Acta Biomater 2018 Feb 13. Epub 2018 Feb 13.
    Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157, USA.
    Bioengineering of a functional cardiac tissue composed of primary cardiomyocytes has great potential for myocardial regeneration and in vitro tissue modeling. However, its applications remain limited because the cardiac tissue is a highly organized structure with unique physiologic, biomechanical, and electrical properties. In this study, we undertook a proof-of-concept study to develop a contractile cardiac tissue with cellular organization, uniformity, and scalability by using three-dimensional (3D) bioprinting strategy. Read More

    pH-sensitive doxorubicin-conjugated prodrug micelles with charge-conversion for cancer therapy.
    Acta Biomater 2018 Feb 13. Epub 2018 Feb 13.
    National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China. Electronic address:
    Intelligent drug delivery systems with prolonged circulation time, reduced drug leakage in blood, target site-triggered drug release and endosomal escape are attractive and ideal for malignant tumor therapy. Herein, doxorubicin (DOX)-conjugated smart polymeric micelles based on 4-carboxy benzaldehyde-grafted poly (L-lysine)-block-poly (methacryloyloxyethyl phosphorylcholine) (PLL(CB/DOX)-b-PMPC) copolymer are prepared. DOX and electronegative 4-carboxy benzaldehyde are conjugated to the PLL block via an imine linkage and as a result, the drug loaded micelles exhibited the pH-triggered charge-conversion property and accelerated drug release at tumor pH. Read More

    A physiologically relevant 3D collagen-based scaffold - neuroblastoma cell system exhibits chemosensitivity similar to orthotopic xenograft models.
    Acta Biomater 2018 Feb 12. Epub 2018 Feb 12.
    Molecular & Cellular Therapeutics, Royal College of Surgeons in Ireland, Dublin, Ireland; National Children's Research Centre, Our Lady's Children's Hospital, Crumlin, Dublin, Ireland. Electronic address:
    3D scaffold-based in vitro cell culturing is a recent technological advancement in cancer research bridging the gap between conventional 2D culture and in vivo tumours. The main challenge in treating neuroblastoma, a paediatric cancer of the sympathetic nervous system, is to combat tumour metastasis and resistance to multiple chemotherapeutic drugs. The aim of this study was to establish a physiologically relevant 3D neuroblastoma tissue-engineered system and explore its therapeutic relevance. Read More

    Mechanically Enhanced Nested-Network Hydrogels as a Coating Material for Biomedical Devices.
    Acta Biomater 2018 Feb 12. Epub 2018 Feb 12.
    Department of Mechanical Engineering, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.. Electronic address:
    Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue present remarkable mechanical strength and play a crucial role in achieving physical and biological functions for living organisms. Inspired by these delicate microstructures in nature, an analogous scaffold of double network hydrogel was fabricated by creating a poly(2-hydroxyethyl methacrylate) (pHEMA) network in the porous structure of alginate hydrogels. The resulting hydrogel possessed hierarchical NN structure and showed significantly improved mechanical strength but still maintained high elasticity comparable to soft tissues due to a mutual strengthening effect between the two networks. Read More

    Effects of maturation and advanced glycation on tensile mechanics of collagen fibrils from rat tail and Achilles tendons.
    Acta Biomater 2018 Feb 12. Epub 2018 Feb 12.
    Institute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital and Center for Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen Denmark; Musculoskeletal Rehabilitation Research Unit, Bispebjerg Hospital, University Hospital of Copenhagen, Copenhagen, Denmark.
    Connective tissues are ubiquitous throughout the body and consequently affect the function of many organs. In load bearing connective tissues like tendon, the mechanical functionality is provided almost exclusively by collagen fibrils that in turn are stabilized by covalent cross-links. Functionally distinct tendons display different cross-link patterns, which also change with maturation, but these differences have not been studied in detail at the fibril level. Read More

    High strength, biodegradable and cytocompatible alpha tricalcium phosphate-iron composites for temporal reduction of bone fractures.
    Acta Biomater 2018 Feb 9. Epub 2018 Feb 9.
    CEITEC - Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
    In this work alpha tricalcium phosphate (α-TCP)/ iron (Fe) composites were developed as a new family of biodegradable, load-bearing and cytocompatible materials. The composites with composition from pure ceramic to pure metallic samples were consolidated by pulsed electric current assisted sintering to minimise processing time and temperature while improving their mechanical performance. The mechanical strength of the composites was increased and controlled with the Fe content, passing from brittle to ductile failure. Read More

    In vitro methods for the evaluation of antimicrobial surface designs.
    Acta Biomater 2018 Feb 10. Epub 2018 Feb 10.
    University of Groningen, University Medical Center Groningen, Department of BioMedical Engineering, Antonius Deusinglaan 1, 9713 AV, Groningen, The Netherlands.
    Bacterial adhesion and subsequent biofilm formation on biomedical implants and devices are a major cause of their failure. As systemic antibiotic treatment is often ineffective, there is an urgent need for antimicrobial biomaterials and coatings. The term "antimicrobial" can encompass different mechanisms of action (here termed "antimicrobial surface designs"), such as antimicrobial-releasing, contact-killing or non-adhesivity. Read More

    A Continuum of Mineralization from Human Renal Pyramid to Stones on Stems.
    Acta Biomater 2018 Feb 8. Epub 2018 Feb 8.
    Department of Urology, University of California San Francisco, San Francisco, CA 94143, United States; Department of Preventive and Restorative Dental Sciences, University of California San Francisco, San Francisco, CA 94143, United States. Electronic address:
    The development of new modalities for kidney stone prevention rests upon understanding the progression of mineralization within the renal pyramid. The progression from small foci of mineralization within the renal pyramid to larger interstitial plaques that ultimately lead to the formation of clinically detectable calcium-based stones on calcium phosphate stems will be presented through correlative microscopy approach. High resolution X-ray computed tomography (micro-XCT), electron microscopy, and energy dispersive X-ray (EDX) compositional analyses of interstitial plaques, stems, and attached stones were performed. Read More

    Enhanced tendon-to-bone repair through adhesive films.
    Acta Biomater 2018 Feb 7. Epub 2018 Feb 7.
    Department of Orthopedic Surgery, Columbia University, New York, NY 10032, United States; Department of Biomedical Engineering, Columbia University, New York, NY 10027, United States. Electronic address:
    Tendon-to-bone surgical repairs have unacceptably high failure rates, possibly due to their inability to recreate the load transfer mechanisms of the native enthesis. Instead of distributing load across a wide attachment footprint area, surgical repairs concentrate shear stress on a small number of suture anchor points. This motivates development of technologies that distribute shear stresses away from suture anchors and across the enthesis footprint. Read More

    Towards the Scale up of Tissue Engineered Intervertebral Discs for Clinical Application.
    Acta Biomater 2018 Feb 7. Epub 2018 Feb 7.
    Translational Musculoskeletal Research Center, Corporal Michael J. Crescenz VA Medical Center, Philadelphia, PA, United States; McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, University of Pennsylvania, Philadelphia, PA, United States. Electronic address:
    Replacement of the intervertebral disc with a viable, tissue-engineered construct that mimics native tissue structure and function is an attractive alternative to fusion or mechanical arthroplasty for the treatment of disc pathology. While a number of engineered discs have been developed, the average size of these constructs remains a fraction of the size of human intervertebral discs. In this study, we fabricated medium (3 mm height x 10 mm diameter) and large (6 mm height x 20 mm diameter) sized disc-like angle ply structures (DAPS), encompassing size scales from the rabbit lumbar spine to the human cervical spine. Read More

    Corrigendum to "Tailoring surface nanoroughness of electrospun scaffolds for skeletal tissue engineering" Acta Biomater. 59 (2017) 82-93.
    Acta Biomater 2018 Feb 6. Epub 2018 Feb 6.
    Department of Complex Tissue Regeneration, MERLN Institute for Technology Inspired Regenerative Medicine, 6200 MD Maastricht, The Netherlands. Electronic address:

    Nanoengineered Injectable Hydrogels from kappa-Carrageenan and Two-dimensional Nanosilicates for Wound Healing Application.
    Acta Biomater 2018 Feb 6. Epub 2018 Feb 6.
    Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843, United States; Department of Materials Sciences, Texas A&M University, College Station, TX 77843, United States; Center for Remote Health Technologies and Systems, Texas A&M University, College Station, TX 77843, USA. Electronic address:
    We report injectable nanoengineered hemostats for enhanced wound healing and tissue regeneration. The nanoengineered system consists of the natural polysaccharide, κ-carrageenan (κCA), loaded with bioactive two-dimensional (2D) nanosilicates. Nanoengineered hydrogels showed shear-thinning characteristics and can be injected for a minimally invasive approach. Read More

    Cell reprogramming by 3D bioprinting of human fibroblasts in polyurethane hydrogel for fabrication of neural-like constructs.
    Acta Biomater 2018 Feb 7. Epub 2018 Feb 7.
    Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan, ROC; Center of Tissue Engineering and 3D Printing, National Taiwan University, Taipei, Taiwan, ROC; Institute of Cellular and System Medicine, National Health Research Institutes, Zhunan, Taiwan, ROC. Electronic address:
    3D bioprinting is a technique which enables the direct printing of biodegradable materials with cells into 3D tissue. So far there is no cell reprogramming in situ performed with the 3D bioprinting process. Forkhead box D3 (FoxD3) is a transcription factor and neural crest marker, which was reported to reprogram human fibroblasts into neural crest stem-like cells. Read More

    Effect of strontium surface-functionalized implants on early and late osseointegration: A histological, spectrometric and tomographic evaluation.
    Acta Biomater 2018 Feb 7. Epub 2018 Feb 7.
    Interdisciplinary Nanoscience Center (iNANO), Faculty of Science and Technology, Aarhus University, Denmark; Department of Physics and Astronomy, Faculty of Science and Technology, Aarhus University, Denmark.
    Numerous in vivo, in vitro and clinical studies report on beneficial effects of strontium with respect to increased bone growth. Based on this knowledge the aim of this study was to evaluate early and late osseointegration stages of functionalized titanium implants showing sustained release of strontium (Sr) and further investigate its potential systemic effect. Strontium functionalized (Ti-Sr-O) and Grade 4 (Control) titanium implants were inserted in the femoral condyle of New Zealand White rabbits. Read More

    Localized delivery of miRNAs targets cyclooxygenases and reduces flexor tendon adhesions.
    Acta Biomater 2018 Feb 7. Epub 2018 Feb 7.
    The Hand Surgery Research Center, Department of Hand Surgery, Affiliated Hospital of Nantong University, Nantong 226001, PR China. Electronic address:
    The formation of adhesions during healing of an injured tendon remains a difficult problem in clinical practice. Local anti-inflammation gene delivery provides high local gene concentration, reduces the inflammatory response of the injured tendon microenvironment, and decreases systemic side effects to enhance in vivo efficacy. In this study, we designed a novel local sustained gene delivery system by using cyclooxygenase (COX-1 and COX-2)-engineered miRNA plasmid/nanoparticles embedded in hyaluronic acid (HA) hydrogel to reduce flexor tendon adhesions. Read More

    Biological connective tissues exhibit viscoelastic and poroelastic behavior at different frequency regimes: Application to tendon and skin biophysics.
    Acta Biomater 2018 Feb 7. Epub 2018 Feb 7.
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA; Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA. Electronic address:
    In this study, a poroviscoelastic finite element model (FEM) was developed and used in conjunction with an AFM-based wide-bandwidth nanorheology system to predict the frequency-dependent mechanical behavior of tendon and dermis subjected to compression via nanoindentation. The aim was to distinguish between loading rates that are dominated by either poroelasticity, viscoelasticity, or the superposition of these processes. Using spherical probe tips having different radii, the force and tip displacement were measured and the magnitude, E, and phase angle, ϕ, of the dynamic complex modulus were evaluated for mouse supraspinatus tendon and mouse dermis. Read More

    Three-dimensional micro-scale strain mapping in living biological soft tissues.
    Acta Biomater 2018 Feb 7. Epub 2018 Feb 7.
    Human Performance Laboratory, Faculty of Kinesiology, University of Calgary, Calgary, Alberta, Canada; Schulich School of Engineering, Biomedical Engineering Graduate Program, University of Calgary, Calgary, Alberta, Canada. Electronic address:
    Non-invasive characterization of the mechanical micro-environment surrounding cells in biological tissues at multiple length scales is important for the understanding of the role of mechanics in regulating the biosynthesis and phenotype of cells. However, there is a lack of imaging methods that allow for characterization of the cell micro-environment in three-dimensional (3D) space. The aims of this study were (i) to develop a multi-photon laser microscopy protocol capable of imprinting 3D grid lines onto living tissue at a high spatial resolution, and (ii) to develop image processing software capable of analyzing the resulting microscopic images and performing high resolution 3D strain analyses. Read More

    Ultrafast glucose-responsive, high loading capacity erythrocyte to self-regulate the release of insulin.
    Acta Biomater 2018 Feb 6. Epub 2018 Feb 6.
    Collaborative Innovation Center of Chemistry for Life Sciences, College of Engineering and Applied Sciences, Nanjing University, Nanjing, Jiangsu 210093, China. Electronic address:
    Insulin (INS) delivery system that can mimic normal insulin secretion to maintain the blood glucose level (BGL) in the normal range is an ideal treatment for diabetes. However, most of the existing closed-loop INS delivery systems respond slowly to the changes in BGL, resulting in a time lag between the abnormal BGL and the release of INS, which is not suitable for practical application. In this study, glucose oxidase (GOx)-modified erythrocytes are used as INS carriers (GOx-INS-ER) that can rapidly self-regulate the release of INS upon the changes in BGL. Read More

    Cyclic RGD functionalized liposomes encapsulating urokinase for thrombolysis.
    Acta Biomater 2018 Feb 9. Epub 2018 Feb 9.
    CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China. Electronic address:
    Thrombosis, a critical event in blood vessels, not only is associated with myocardial infarction and stroke, but also accounts for considerable morbidity and mortality. Thrombolytic drugs are usually applied to the treatment of acute myocardial infarction, acute cerebral infarction and pulmonary embolism. However, thrombolytic drugs show limited efficacy in clinical practice because of the short half-life in plasma and systemic side effects. Read More

    Lung cancer specific and reduction-responsive chimaeric polymersomes for highly efficient loading of pemetrexed and targeted suppression of lung tumor in vivo.
    Acta Biomater 2018 Feb 6. Epub 2018 Feb 6.
    Biomedical Polymers Laboratory, and Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, PR China. Electronic address:
    Lung cancer is one of the worldwide leading and fast-growing malignancies. Pemetrexed disodium (PEM, Alimta®), a small hydrophilic drug, is currently used for treating lung cancer patients. However, PEM suffers from issues like fast elimination, low bioavailability, poor tumor cell selectivity and penetration. Read More

    Independent control of matrix adhesiveness and stiffness within a 3D self-assembling peptide hydrogel.
    Acta Biomater 2018 Feb 2. Epub 2018 Feb 2.
    The Ohio State University, Department of Biomedical Engineering, 270 Bevis Hall 1080 Carmack Rd., Columbus, OH 43210, USA; The Ohio State University, Davis Heart Lung Research Institute, 473 W 12th Ave., Columbus, OH 43210, USA. Electronic address:
    A cell's insoluble microenvironment has increasingly been shown to exert influence on its function. In particular, matrix stiffness and adhesiveness strongly impact behaviors such as cell spreading and differentiation, but materials that allow for independent control of these parameters within a fibrous, stromal-like microenvironment are very limited. In the current work, we devise a self-assembling peptide (SAP) system that facilitates user-friendly control of matrix stiffness and RGD (Arg-Gly-Asp) concentration within a hydrogel possessing a microarchitecture similar to stromal extracellular matrix. Read More

    Silk fibroin/collagen protein hybrid cell-encapsulating hydrogels with tunable gelation and improved physical and biological properties.
    Acta Biomater 2018 Feb 2. Epub 2018 Feb 2.
    Institute of Tissue Regeneration Engineering (ITREN), Dankook University, South Korea; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, South Korea; Department of Biomaterials Science, College of Dentistry, Dankook University, South Korea. Electronic address:
    Cell encapsulating hydrogels with tunable mechanical and biological properties are of special importance for cell delivery and tissue engineering. Silk fibroin and collagen, two typical important biological proteins, are considered potential as cell culture hydrogels. However, both have been used individually, with limited properties (e. Read More

    Linking multiscale deformation to microstructure in cortical bone using in situ loading, digital image correlation and synchrotron X-ray scattering.
    Acta Biomater 2018 Feb 2. Epub 2018 Feb 2.
    Department of Biomedical Engineering, Lund University, Box 118, SE-221 00 Lund, Sweden. Electronic address:
    The incidence of fragility fractures is expected to increase in the near future due to an aging population. Therefore, improved tools for fracture prediction are required to treat and prevent these injuries efficiently. For such tools to succeed, a better understanding of the deformation mechanisms in bone over different length scales is needed. Read More

    Structure and collagen crimp patterns of functionally distinct equine tendons, revealed by quantitative polarised light microscopy (qPLM).
    Acta Biomater 2018 Feb 2. Epub 2018 Feb 2.
    School of Engineering and Materials Science, Queen Mary University of London, Mile End Rd, London E1 4NS, United Kingdom. Electronic address:
    Structure-function relationships in tendons are directly influenced by the arrangement of collagen fibres. However, the details of such arrangements in functionally distinct tendons remain obscure. This study demonstrates the use of quantitative polarised light microscopy (qPLM) to identify structural differences in two major tendon compartments at the mesoscale: fascicles and interfascicular matrix (IFM). Read More

    Bone regeneration capacity of magnesium phosphate cements in a large animal model.
    Acta Biomater 2018 Feb 2. Epub 2018 Feb 2.
    Institute for Orthopaedic Research and Biomechanics, Trauma Research Center Ulm, University of Ulm, Helmholtzstrasse 14, D-89081 Ulm, Germany.
    Magnesium phosphate minerals have captured increasing attention during the past years as suitable alternatives for calcium phosphate bone replacement materials. Here, we investigated the degradation and bone regeneration capacity of experimental struvite (MgNHPO·6HO) forming magnesium phosphate cements in two different orthotopic ovine implantation models. Cements formed at powder to liquid ratios (PLR) of 2. Read More

    Introduction of sacrificial bonds to hydrogels to increase defect tolerance during suturing of multilayer vascular grafts.
    Acta Biomater 2018 Feb 2. Epub 2018 Feb 2.
    Department of Biomedical Engineering, University of Texas, Austin, TX 78712, United States. Electronic address:
    Small-caliber vascular grafts used in coronary artery bypass procedures typically fail due to the development of intimal hyperplasia or thrombosis. Our laboratory has developed a multilayered vascular graft with an electrospun polyurethane outer layer with improved compliance matching and a hydrogel inner layer that is both thromboresistant and promotes endothelialization. Initial in vivo studies showed that hydrogel particulates were dislodged from the hydrogel layer of the grafts during suturing. Read More

    Delayed release of chemokine CCL25 with bioresorbable microparticles for mobilization of human mesenchymal stem cells.
    Acta Biomater 2018 Feb 2. Epub 2018 Feb 2.
    Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Tissue Engineering Laboratory and Berlin-Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charitéplatz 1, 10117 Berlin, Germany; Department of Materials Science and Technologies, Chair of Advanced Ceramic Materials, Technische Universität Berlin, Hardenbergstrasse 40, 10623 Berlin, Germany.
    Chemokines are guiding cues for directional trafficking of mesenchymal stem cells (MSC) upon injury and local chemokine delivery at injury sites is an up-to-date strategy to potentiate and prolong recruitment of MSC. In this study we present the chemokine CCL25, also referred to as thymus-expressed chemokine, to mobilize human MSC along positive but not along negative gradients. We hence proceeded to design a biodegradable and injectable release device for CCL25 on the basis of poly(lactic-co-glycolic acid) (PLGA). Read More

    The rheological properties of native sericin.
    Acta Biomater 2018 Feb 1. Epub 2018 Feb 1.
    Department of Materials Science and Engineering, The University of Sheffield, Sir Robert Hadfield Building, Mappin Street, Sheffield, S. Yorks S1 3JD, UK. Electronic address:
    Unlike spider silk, spinning silkworm silk has the added intricacy of being both fibre and micron-thick glue-like coating. Whilst the natural flow properties of the fibre feedstock fibroin are now becoming more established, our understanding of the coating sericin is extremely limited and thus presents both a gap in our knowledge and a hindrance to successful exploitation of these materials. In this study we characterise sericin feedstock from the silkworm Bombyx mori in its native state and by employing both biochemical, rheological and spectroscopic tools, define a natural gold standard. Read More

    Targeted delivery of FGF2 to subchondral bone enhanced the repair of articular cartilage defect.
    Acta Biomater 2018 Feb 2. Epub 2018 Feb 2.
    Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Peking Union Medical College, The Key Laboratory of Biomedical Material of Tianjin, Tianjin 300192, PR China. Electronic address:
    It is reported that growth factor (GF) is able to enhance the repair of articular cartilage (AC) defect, however underlying mechanisms of which are not fully elucidated yet. Moreover, the strategy for delivering GF needs to be optimized. The crosstalk between AC and subchondral bone (SB) play important role in the homeostasis and integrity of AC, therefore SB targeted delivery of GF represents one promising way to facilitate the repair of AC defect. Read More

    Predicting patient exposure to nickel released from cardiovascular devices using multi-scale modeling.
    Acta Biomater 2018 Feb 13. Epub 2018 Feb 13.
    Center for Devices and Radiological Health, FDA, Silver Spring, MD 20993, United States.
    Many cardiovascular device alloys contain nickel, which if released in sufficient quantities, can lead to adverse health effects. However, in-vivo nickel release from implanted devices and subsequent biodistribution of nickel ions to local tissues and systemic circulation are not well understood. To address this uncertainty, we have developed a multi-scale (material, tissue, and system) biokinetic model. Read More

    Carrier-free nanodrug by co-assembly of chemotherapeutic agent and photosensitizer for cancer imaging and chemo-photo combination therapy.
    Acta Biomater 2018 Feb 5. Epub 2018 Feb 5.
    Cancer Metastasis Alert and Prevention Center, Pharmaceutical Photocatalysis of State Key Laboratory of Photocatalysis on Energy and Environment, and Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, College of Chemistry, Fuzhou University, Fuzhou 350116, China. Electronic address:
    Nanosized drug delivery systems (NDDS) with photothermal therapy (PTT) and photodynamic therapy (PDT) have been extensively exploited to improve the therapeutic performance and bio-safety of chemotherapeutic drugs in cancer. In this work, a carrier-free nanodrug was developed by co-assembly of the anti-cancer agent ursolic acid (UA), an asialoglycoprotein receptor (ASGPR), which can recognize the target molecule lactobionic acid (LA), and the near-infrared (NIR) probe dye indocyanine green (ICG) to form UA-LA-ICG NPs by a simple and green self-assembly approach. The UA-LA-ICG NPs had suitable stability, showed controlled release profile of UA drugs, and exhibited preferable temperature response (∼59. Read More

    Microgels produced using microfluidic on-chip polymer blending for controlled released of VEGF encoding lentivectors.
    Acta Biomater 2018 Feb 1. Epub 2018 Feb 1.
    Department of Biomedical Engineering, University of California, Davis, CA, USA. Electronic address:
    Alginate hydrogels are widely used as delivery vehicles due to their ability to encapsulate and release a wide range of cargos in a gentle and biocompatible manner. The release of encapsulated therapeutic cargos can be promoted or stunted by adjusting the hydrogel physiochemical properties. However, the release from such systems is often skewed towards burst-release or lengthy retention. Read More

    Highly efficient local delivery of endothelial progenitor cells significantly potentiates angiogenesis and full-thickness wound healing.
    Acta Biomater 2018 Jan 31. Epub 2018 Jan 31.
    Key Laboratory of Orthopedics of Zhejiang Province, Department of Orthopedics, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China; National Engineering Research Center for Tissues Restoration and Reconstruction, South China University of Technology, Guangzhou 510640, China. Electronic address:
    Wound therapy with a rapid healing performance remains a critical clinical challenge. Cellular delivery is considered to be a promising approach to improve the efficiency of healing, yet problems such as compromised cell viability and functionality arise due to the inefficient delivery. Here, we report the efficient delivery of endothelial progenitor cells (EPCs) with a bioactive nanofibrous scaffold (composed of collagen and polycaprolactone and bioactive glass nanoparticles, CPB) for enhancing wound healing. Read More

    Challenges in vascular tissue engineering for diabetic patients.
    Acta Biomater 2018 Jan 25. Epub 2018 Jan 25.
    Clemson University, Department of Bioengineering, United States. Electronic address:
    Hyperglycemia and dyslipidemia coexist in diabetes and result in inflammation, degeneration, and impaired tissue remodeling, processes which are not conducive to the desired integration of tissue engineered products into the surrounding tissues. There are several challenges for vascular tissue engineering such as non-thrombogenicity, adequate burst pressure and compliance, suturability, appropriate remodeling responses, and vasoactivity, but, under diabetic conditions, an additional challenge needs to be considered: the aggressive oxidative environment generated by the high glucose and lipid concentrations that lead to the formation of advanced glycation end products (AGEs) in the vascular wall. Extracellular matrix-based scaffolds have adequate physical properties and are biocompatible, however, these scaffolds are altered in diabetes by the formation AGEs and impaired collagen degradation, consequently increasing vascular wall stiffness. Read More

    Interplay between stiffness and degradation of architectured gelatin hydrogels leads to differential modulation of chondrogenesis in vitro and in vivo.
    Acta Biomater 2018 Jan 31. Epub 2018 Jan 31.
    Institute for Macromolecular Chemistry, University of Freiburg, 79104 Freiburg, Germany; Helmholtz Virtual Institute on Multifunctional Biomaterials for Medicine, Kantstr. 55, 14513 Teltow, Germany; BIOSS, Centre for Biological Signalling Studies, University of Freiburg, 79104 Freiburg, Germany. Electronic address:
    The limited capacity of cartilage to heal large lesions through endogenous mechanisms has led to extensive effort to develop materials to facilitate chondrogenesis. Although physical-chemical properties of biomaterials have been shown to impact in vitro chondrogenesis, whether these findings are translatable in vivo is subject of debate. Herein, architectured 3D hydrogel scaffolds (ArcGel) (produced by crosslinking gelatin with ethyl lysine diisocyanate (LDI)) were used as a model system to investigate the interplay between scaffold mechanical properties and degradation on matrix deposition by human articular chondrocytes (HAC) from healthy donors in vitro and in vivo. Read More

    Capacity of octacalcium phosphate to promote osteoblastic differentiation toward osteocytes in vitro.
    Acta Biomater 2018 Feb 3. Epub 2018 Feb 3.
    Division of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan. Electronic address:
    Octacalcium phosphate (OCP) has been shown to act as a nucleus for initial bone deposition and enhancing the early stages of osteoblastic differentiation. However, the effect on differentiation at the late stage into osteocytes has not been elucidated. The present study was designed to investigate whether OCP can promote the differentiation lineage from osteoblasts to late osteocytes using a clonal cell line IDG-SW3 compared to commercially available sintered β-tricalcium phosphate (β-TCP) and hydroxyapatite (HA) in a transwell cell culture. Read More

    Adhesive free-standing multilayer films containing sulfated levan for biomedical applications.
    Acta Biomater 2018 Jan 31. Epub 2018 Jan 31.
    3B's Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark-Parque de Ciência e Tecnologia, 4805-017 Barco, Taipas, Guimarães, Portugal; ICVS/3B's, Associate PT Government Laboratory, Braga/Guimarães, Portugal; Department of Chemistry, CICECO - Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, Portugal(2). Electronic address:
    This work is the first reporting the use of layer-by-layer to produce adhesive free-standing (FS) films fully produced using natural-based macromolecules: chitosan (CHI), alginate (ALG) and sulfated levan (L-S). The deposition conditions of the natural polymers were studied through zeta potential measurements and quartz crystal microbalance with dissipation monitoring analysis. The properties of the FS films were evaluated and compared with the control ones composed of only CHI and ALG in order to assess the influence of levan polysaccharide introduced in the multilayers. Read More

    Computational 3D imaging to quantify structural components and assembly of protein networks.
    Acta Biomater 2018 Jan 31. Epub 2018 Jan 31.
    Institute of Applied Mechanics, University of Stuttgart, Stuttgart, Germany; Stuttgart Research Centre for Simulation Technology (SimTech), Stuttgart, Germany.
    Traditionally, protein structures have been described by the secondary structure architecture and fold arrangement. However, the relatively novel method of 3D confocal microscopy of fluorescent-protein-tagged networks in living cells allows resolving the detailed spatial organization of these networks. This provides new possibilities to predict network functionality, as structure and function seem to be linked at various scales. Read More

    Corneal regeneration: A review of stromal replacements.
    Acta Biomater 2018 Jan 31. Epub 2018 Jan 31.
    University of Antwerp, Ophthalmology, Visual Optics and Visual Rehabilitation, Universiteitsplein 1, Building T4, 2610 Wilrijk, Belgium; Department of Ophthalmology, Antwerp University Hospital, Wilrijkstraat 10, 2650 Edegem, Belgium; Center for Cell Therapy and Regenerative Medicine, Antwerp University Hospital, U113 Wilrijkstraat 10, 2650 Edegem, Belgium. Electronic address:
    Corneal blindness is traditionally treated by transplantation of a donor cornea, or in severe cases by implantation of an artificial cornea or keratoprosthesis. Due to severe donor shortages and the risks of complications that come with artificial corneas, tissue engineering in ophthalmology has become more focused on regenerative strategies using biocompatible materials either with or without cells. The stroma makes up the bulk of the corneal thickness and mainly consists of a tightly interwoven network of collagen type I, making it notoriously difficult to recreate in a laboratory setting. Read More

    pH-triggered charge-reversible of glycol chitosan conjugated carboxyl graphene for enhancing photothermal ablation of focal infection.
    Acta Biomater 2018 Jan 31. Epub 2018 Jan 31.
    Institute of Burn Research, Southwest Hospital, State Key Lab of Trauma, Burn and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China. Electronic address:
    Subcutaneous abscesses infected by multidrug-resistant bacteria are becoming an increasing challenge to human health. To address this challenge, a surface-adaptive and biocompatible glycol chitosan conjugated carboxyl graphene (GCS-CG) is developed, which exhibits unique self-adaptive target to the acidic microenvironment of abscess (∼pH 6.3) and no damage to the healthy tissue (pH 7. Read More

    Integrated approaches to spatiotemporally directing angiogenesis in host and engineered tissues.
    Acta Biomater 2018 Jan 31. Epub 2018 Jan 31.
    Center for Biomedical Engineering, School of Engineering, Brown University, Providence, RI, USA. Electronic address:
    The field of tissue engineering has turned towards biomimicry to solve the problem of tissue oxygenation and nutrient/waste exchange through the development of vasculature. Induction of angiogenesis and subsequent development of a vascular bed in engineered tissues is actively being pursued through combinations of physical and chemical cues, notably through the presentation of topographies and growth factors. Presenting angiogenic signals in a spatiotemporal fashion is beginning to generate improved vascular networks, which will allow for the creation of large and dense engineered tissues. Read More

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