6,636 results match your criteria Acta biomaterialia[Journal]


Decellularized Tissues as Platforms for In Vitro Modeling of Healthy and Diseased Tissues.

Acta Biomater 2020 May 25. Epub 2020 May 25.

J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 1275 Center Dr. BMS J257, Gainesville, FL USA 32611. Electronic address:

Biomedical engineers are at the forefront of developing novel treatments to improve human health, however, many products fail to translate to clinical implementation. In vivo pre-clinical animal models, although the current best approximation of complex disease conditions, are limited by reproducibility, ethical concerns, and poor accurate prediction of human response. Hence, there is a need to develop physiologically relevant, low cost, scalable, and reproducible in vitro platforms to provide reliable means for testing drugs, biomaterials, and tissue engineered products for successful clinical translation. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.031DOI Listing

Collagen hydrogel confinement of Amyloid-β (Aβ) accelerates aggregation and reduces cytotoxic effects.

Acta Biomater 2020 May 25. Epub 2020 May 25.

Department of Chemical, Biochemical and Environmental Engineering, University of Maryland Baltimore County, Baltimore, Maryland, USA. Electronic address:

Alzheimer's disease (AD) is the most common form of dementia and is associated with the accumulation of amyloid-β (Aβ), a peptide whose aggregation has been associated with neurotoxicity. Drugs targeting Aβ have shown great promise in 2D in vitro models and mouse models, yet preclinical and clinical trials for AD have been highly disappointing. We propose that current in vitro culture systems for discovering and developing AD drugs have significant limitations; specifically, that Aβ aggregation is vastly different in these 2D cultures carried out on flat plastic or glass substrates vs. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.030DOI Listing

Self-assembling Prodrug Nanotherapeutics for Synergistic Tumor Targeted Drug Delivery.

Acta Biomater 2020 May 23. Epub 2020 May 23.

Skaggs Pharmaceutical Sciences Center, Department of Pharmacology & Toxicology, College of Pharmacy, The University of Arizona, Tucson, Arizona, 85721, United States; NCI-designated University of Arizona Comprehensive Cancer Center, Tucson, Arizona, 85721, United States; Southwest Environmental Health Sciences Center, The University of Arizona, Tucson, 85721, United States; BIO5 Institute, The University of Arizona, Tucson, Arizona, 85721, United States. Electronic address:

Self-assembling prodrugs represents a robust and effective nanotherapeutic approach for delivering poorly soluble anticancer drugs. With numerous intrinsic advantages, self-assembling prodrugs possess the maximum drug loading capacity, controlled drug release kinetics, prolonged blood circulation, and preferential tumor accumulation based on the enhanced permeability and retention (EPR) effect. These prodrug conjugates allow for efficient self-assembly into nanodrugs with the potential of encapsulating other therapeutic agents that have different molecular targets, enabling simultaneous temporal-spatial release of drugs for synergistic antitumor efficacy with reduced systemic side effects. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245299PMC

Type IV collagen conforms to the organization of polylaminin adsorbed on planar substrata.

Acta Biomater 2020 May 22. Epub 2020 May 22.

ICREA and Institute for Bioengineering of Catalonia (IBEC) Barcelona, Spain. Electronic address:

Tissue engineering demands the development of scaffolds that mimic natural extracellular matrices (ECM). Despite the success in obtaining synthetic interstitial ECM, the production of an artificial basement membrane (BM), the specialized thin sheet of ECM that is pivotal for the functional organization of most tissues and internal organs, is still not achieved. With the long-term aim of developing a flat BM-like structure here we investigated the behavior of acid-soluble Col IV during simultaneous assembly with laminin (LM) in acidic conditions. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.021DOI Listing

Amifostine-loaded armored dissolving microneedles for long-term prevention of ionizing radiation-induced injury.

Acta Biomater 2020 May 22. Epub 2020 May 22.

Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China; Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China. Electronic address:

Amifostine is a cytoprotective agent against the hematopoietic damage induced by ionizing radiation, although the intravenous injection of amifostine is a unique administration method with strict dosing time limitation. Hence, the fields of application of amifostine are greatly limited. Here, we have developed an amifostine-loaded armored microneedle (AAMN) with long-term prevention of hematopoietic injury induced by ionizing radiation. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.025DOI Listing

Continuous microfluidic encapsulation of single mesenchymal stem cells using alginate microgels as injectable fillers for bone regeneration.

Acta Biomater 2020 May 22. Epub 2020 May 22.

State key laboratory of fine chemicals, School of Bioengineering, Dalian University of Technology, Dalian 116023, P. R. China. Electronic address:

The encapsulation of cells in microscale hydrogels can provide a mimic of a three-dimensional (3D) microenvironment to support cell viability and functions and to protect cells from the environmental stress, which have been widely used in tissue regeneration and cell therapies. Here, a microfluidics-based approach is developed for continuous encapsulation of mesenchymal stem cells (MSCs) at the single-cell level using alginate microgels. This microfluidic technique integrated on-chip encapsulation, gelation, and de-emulsification into a one-step fabrication process, which enables scalable cell encapsulation while retaining the viability and functionality of loaded cells. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.024DOI Listing

Microwave processing of Calcium Phosphate and Magnesium Phosphate based Orthopedic Bioceramics: A State-of-the-Art Review.

Acta Biomater 2020 May 21. Epub 2020 May 21.

Department of Mechanical, Industrial and Manufacturing Engineering, The University of Toledo, Toledo, OH, 43606, USA; Currently at EEC (ENG) Division, The National Science Foundation (NSF), Alexandria, VA, 22314, USA.

The main theme of this paper is to review microwave-assisted synthesis and processing of calcium and magnesium phosphate bioceramics. Microwave processing of advanced materials has been an active field of research for the last three decades and has been already reviewed in the literature. Microwave processing of bioceramics is being pursued for almost the same period of time. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.018DOI Listing

Antibiotic eluting poly(ester urea) films for control of a model cardiac implantable electronic device infection.

Acta Biomater 2020 May 21. Epub 2020 May 21.

Department of Chemistry, Mechanical Engineering and Materials Science, Orthopaedic Surgery, and Biomedical Engineering, Duke University, Durham, NC, 27708, United States. Electronic address:

Cardiac implantable electronic device (CIED) infections acquired during or after surgical procedures are a major complication that are challenging to treat therapeutically, resulting in chronic and sometimes fatal infections. Localized delivery of antibiotics at the surgical site could be used to supplement traditional systemic administration as a preventative measure. Herein, we investigate an cefazolin-eluting l-valine poly(ester urea) (PEU) films as a model system for localized antibiotic delivery for CIEDs. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.025DOI Listing

Ex vivo evaluation of the blood compatibility of Mixed Matrix haemodialysis Membranes.

Acta Biomater 2020 May 21. Epub 2020 May 21.

(Bio)artificial organs, Department of Biomaterials Science and Technology, TechMed Centre, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands. Electronic address:

The patients with end stage kidney disease need haemodialysis therapies, using an artificial kidney. Nevertheless, the current therapies cannot remove a broad range of uremic toxins compared to the natural kidney. Adsorption therapies, using sorbent-based columns, can improve the clearance of uremic toxins, but the sorbent particles often require polymeric coatings to improve their haemocompatibility leading to mass transfer limitations and to lowering of their performance. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.016DOI Listing

Host macrophage response to injectable hydrogels derived from ECM and α-helical peptides.

Acta Biomater 2020 May 21. Epub 2020 May 21.

McGowan Institute for Regenerative Medicine, University of Pittsburgh, 450 Technology Drive, Suite 300, Pittsburgh, PA 15219-3110, USA; Department of Surgery, School of Medicine, University of Pittsburgh, University of Pittsburgh Medical Center Presbyterian Hospital, 200 Lothrop Street, Pittsburgh, PA 15213, USA; Department of Bioengineering, University of Pittsburgh, 3700 O'Hara Street, Pittsburgh, PA, 15261, USA.

Tissue engineering materials play a key role in how closely the complex architectural and functional characteristics of native healthy tissue can be replicated. Traditional natural and synthetic materials are superseded by bespoke materials that cross the boundary between these two categories. Here we present hydrogels that are derived from decellularised extracellular matrix and those that are synthesised from de novo α-helical peptides. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.022DOI Listing

Sacrificial Fibers Improve Matrix Distribution and Micromechanical Properties in a Tissue-Engineered Intervertebral Disc.

Acta Biomater 2020 May 21. Epub 2020 May 21.

Translational Musculoskeletal Research Center, Corporal Michael J. Crescenz VA Medical Center, Philadelphia, PA; McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, University of Pennsylvania, Philadelphia, PA. Electronic address:

Tissue-engineered replacement discs are an area of intense investigation for the treatment of end-stage intervertebral disc (IVD) degeneration. These living implants can integrate into the IVD space and recapitulate native motion segment function. We recently developed a multiphasic tissue-engineered disc-like angle-ply structure (DAPS) that models the micro-architectural and functional features of native tissue. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.019DOI Listing

Extracellular vesicles from human urine-derived stem cells inhibit glucocorticoid-induced osteonecrosis of the femoral head by transporting and releasing pro-angiogenic DMBT1 and anti-apoptotic TIMP1.

Acta Biomater 2020 May 21. Epub 2020 May 21.

Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Hunan Key Laboratory of Organ Injury, Aging and Regenerative Medicine, Changsha, Hunan 410008, China; Hunan Key Laboratory of Bone Joint Degeneration and Injury, Changsha, Hunan 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Key Laboratory of Biological Nanotechnology of National Health Commission, Changsha, Hunan 410008, China. Electronic address:

Osteonecrosis of the femoral head (ONFH) frequently occurs after glucocorticoid (GC) treatment. Extracellular vesicles (EVs) are important nano-sized paracrine mediators of intercellular crosstalk. This study aimed to determine whether EVs from human urine-derived stem cells (USC-EVs) could protect against GC-induced ONFH and focused on the impacts of USC-EVs on angiogenesis and apoptosis to explore the mechanism by which USC-EVs attenuated GC-induced ONFH. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.020DOI Listing

An "all-in-one" scaffold targeting macrophages to direct endogenous bone repair in situ.

Acta Biomater 2020 May 21. Epub 2020 May 21.

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Avenida da Universidade, Macau SAR, China. Electronic address:

Scaffolds for tissue repair are designed in an increasingly complicated manner to meet multi-facet biological needs during the healing process. However, overly sophisticated design, especially the use of multiple components and delivery of exogenous cells, hampers the bench-to-bedside translation. Here, a multi-functional - yet mono-compositional - bioactive scaffold is devised to mediate the full-range, endogenous bone repair. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.023DOI Listing

Biomimetic and Bioinspired Silicifications: Recent Advances for Biomaterial Design and Applications.

Acta Biomater 2020 May 21. Epub 2020 May 21.

Department of Biotechnology and Bioinformatics, Korea University, Sejong-Ro 2511, Sejong, 30019, Republic of Korea. Electronic address:

The rational design and controllable synthesis of functional silica-based materials have gained increased interest in a variety of biomedical and biotechnological applications due to their unique properties. The current review shows that marine organisms, such as siliceous sponges and diatoms, could be the inspiration for the fabrication of advanced biohybrid materials. Several biomolecules were involved in the molecular mechanism of biosilicification in vivo. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.017DOI Listing

Past, Present, and Future of Affinity-based Cell Separation Technologies.

Acta Biomater 2020 May 19. Epub 2020 May 19.

Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA; Biomanufacturing Training and Education Center (BTEC), North Carolina State University, Raleigh, NC 27695-7928, USA. Electronic address:

Progress in cell purification technology is critical to increase the availability of viable cells for therapeutic, diagnostic, and research applications. A variety of techniques are now available for cell separation, ranging from non-affinity methods such as density gradient centrifugation, dielectrophoresis, and filtration, to affinity methods such as chromatography, two-phase partitioning, and magnetic-/fluorescence-assisted cell sorting. For clinical and analytical procedures that require highly purified cells, the choice of cell purification method is crucial, since every method offers a different balance between yield, purity, and bioactivity of the cell product. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.004DOI Listing

An atorvastatin calcium and poly(L-lactide-co-caprolactone) core-shell nanofiber-covered stent to treat aneurysms and promote reendothelialization.

Acta Biomater 2020 May 19. Epub 2020 May 19.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P. R. China. Electronic address:

Aneurysmal subarachnoid hemorrhage is a common complication caused by an intracranial aneurysm that can lead to hemorrhagic stroke, brain damage, and death. Knowing this clinical situation, the purpose of this study was to develop a controlled-release stent covered with a core-shell nanofiber mesh, fabricated by emulsion electrospinning, for the treatment of aneurysms. By encapsulating atorvastatin calcium (AtvCa) in the inner of poly (L-lactide-co-caprolactone) (PLCL) nanofibers, the release period of AtvCa was effectively extended. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.044DOI Listing

Harnessing the perinuclear actin cap (pnAC) to influence nanocarrier trafficking and gene transfection efficiency in skeletal myoblasts using nanopillars.

Acta Biomater 2020 May 19. Epub 2020 May 19.

Shenzhen Key Laboratory of Biomimetic Materials and Cellular Immunomodulation, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China; Department of Chemistry and Biotechnology, Swinburne University of Technology, Hawthorn, Victoria 3122, Australia. Electronic address:

Gene transfection is important in biotechnology and is used to modify cells intrinsically. It can be conducted in cell suspension or after cell adhesion, where the efficiency is dependent on many factors such as the type of nanocarrier used and cell division processes. Anchor-dependent cells are sensitive to the substrate they are attached to and adapt their behavior accordingly, including plasmid trafficking during gene transfection. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.015DOI Listing

A pH-sensitive fluorescent protein sensor to follow the pathway of calcium phosphate nanoparticles into cells.

Acta Biomater 2020 May 18. Epub 2020 May 18.

Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Universitaetsstr. 5-7, 45117 Essen, Germany. Electronic address:

Calcium phosphate nanoparticles (100 nm) were fluorescently labelled with poly(ethyleneimine) (PEI; red fluorescence). They were loaded with a Tandem fusion protein consisting of mRFP1-eGFP (red and green fluorescence in the same molecule)that acts as smart biological pH sensor to trace nanoparticles inside cells. Its fluorescence is also coupled to the structural integrity of the protein, i. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.014DOI Listing

Polymeric nanoparticles protect the resin-dentin bonded interface from cariogenic biofilm degradation.

Acta Biomater 2020 May 18. Epub 2020 May 18.

MDRCBB-Minnesota Dental Research Center for Biomaterials and Biomechanics, Department of Restorative Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN, USA.

The objective was to assess doxycycline (Dox) and zinc (Zn) doped nanoparticles' (NPs) potential to protect the resin-dentin interface from cariogenic biofilm. Three groups of polymeric NPs were tested: unloaded, loaded with zinc and with doxycycline. NPs were applied after dentin etching. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.002DOI Listing

Influence of Proteins on Mechanical Properties of a Natural Chitin-Protein Composite.

Acta Biomater 2020 May 18. Epub 2020 May 18.

Dipartimento di Chimica "Giacomo Ciamician", University of Bologna, via F. Selmi 2, 40126 Bologna, Italy.

In many biogenic materials, chitin chains are assembled in fibrils that are wrapped by a protein fold. In them, the mechanical properties are supposed to relate to intra- and inter- interactions among chitin and proteins. This hypothesis has been poorly investigated. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.039DOI Listing

Bio-inspired apatite particles limit skin penetration of drugs for dermatology applications.

Acta Biomater 2020 May 18. Epub 2020 May 18.

CIRIMAT Carnot Institute, UMR CNRS/INPT/UPS 5085, University of Toulouse, Ensiacet, 4 allée E. Monso, 31030 Toulouse cedex 4, France. Electronic address:

Most treatments of skin pathologies involve local administration of active agents. One issue can however be the partial transcutaneous diffusion of the drug to blood circulation, leading to undesirable effects. In this work, the original use of submicron mineral particles based on bio-inspired calcium phosphate apatite was explored for the first time as drug carriers for favoring topical delivery. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.010DOI Listing

Co-delivery of doxorubicin and DNAzyme using ZnO@polydopamine core-shell nanocomposites for chemo/gene/photothermal therapy.

Acta Biomater 2020 May 11. Epub 2020 May 11.

Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, Hunan, China; Department of Nuclear Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China; Key Laboratory of Biological Nanotechnology of National Health Commission, Changsha 410008, Hunan, China. Electronic address:

Multi-modal nanomedicines that synergistically combine chemo-, gene-, and photothermal therapy have shown great potential for cancer treatment. In this study, a core-shell nanosystem-based on a zinc oxide (ZnO) nanocore and a polydopamine (PDA) shell was constructed to integrate chemo- (doxorubicin, DOX), gene- (DNAzyme, DZ), and photothermal (PDA layer) therapy in one system. Instead of small interfering RNAs, we employed DZ for tumor-related gene (survivin) regulation owing to its higher stability, biocompatibility, and predictable activity. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.041DOI Listing

Mechanical Resistance in Decapod Claw Denticles: Contribution of Structure and Composition.

Acta Biomater 2020 May 11. Epub 2020 May 11.

Department of Biology, The College of New Jersey, Ewing, NJ, 08628, USA. Electronic address:

The decapod crustacean exoskeleton is a multi-layered structure composed of chitin-protein fibers embedded with calcium salts. Decapod claws display tooth-like denticles, which come into direct contact with predators and prey. They are subjected to more regular and intense mechanical stress than other parts of the exoskeleton and therefore must be especially resistant to wear and abrasion. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.037DOI Listing

Dual dynamically crosslinked thermosensitive hydrogel with self-fixing as a postoperative anti-adhesion barrier.

Acta Biomater 2020 May 10. Epub 2020 May 10.

School of Materials Science and Engineering, Key Laboratory of Polymeric Composite Materials and Functional Materials of Ministry of Education, GD Research Center for Functional Biomaterials Engineering and Technology, Sun Yat-sen University, Guangzhou 510275, China. Electronic address:

Tissue adhesion is a severe postoperative complication. Various strategies have been developed to minimize postoperative adhesion, but the clinical efficacy is still far from satisfactory. Herein, we present a dual dynamically crosslinked hydrogel to serve as a physical postoperative anti-adhesion barrier. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.034DOI Listing

Structure and composition of the tunic in the sea pineapple Halocynthia roretzi: A complex cellulosic composite biomaterial.

Acta Biomater 2020 May 10. Epub 2020 May 10.

Biology of Marine Organisms and Biomimetics Unit, Research Institute for Biosciences, University of Mons, 23 Place du Parc, 7000, Mons, Belgium. Electronic address:

Biological organisms produce high-performance composite materials, such as bone, wood and insect cuticle, which provide inspiration for the design of novel materials. Ascidians (sea squirts) produce an organic exoskeleton, known as a tunic, which has been studied quite extensively in several species. However, currently, there are still gaps in our knowledge about the detailed structure and composition of this cellulosic biocomposite. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.038DOI Listing

On the amorphous layer in bone mineral and biomimetic apatite: a combined small- and wide-angle X-ray scattering analysis.

Acta Biomater 2020 May 10. Epub 2020 May 10.

Institute of Crystallography and To.Sca.Lab. Consiglio Nazionale delle Ricerche, Via Valleggio 11, I-22100 Como, Italy. Electronic address:

The occurrence of an amorphous calcium phosphate layer covering the crystalline apatite core has been suggested to be an intrinsic feature of both bone mineral and synthetic biomimetic analogs. However, an exahustive quantitative picture of the amorphous-crystalline relationship in these materials is still missing. Here, we present a multiple scale modelling that combines small-angle X-ray scattering (SAXS) and synchrotron wide-angle X-ray total scattering (WAXTS) analyses to investigate the amorphous-crystalline spatial interplay in bone sample and biomimetic carbonated nano-apatites. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.026DOI Listing

Transplantation of autologous oral mucosal epithelial cell sheets inhibits the development of acquired external auditory canal atresia in a rabbit model.

Acta Biomater 2020 May 11. Epub 2020 May 11.

Department of Otorhinolaryngology, St. Marianna University School of Medicine, 2-16-1, Sugao, Miymaeku Kanagawa, Japan. Electronic address:

Acquired external auditory canal atresia is characterized by fibrous tissue formation in the ear canal, hearing loss and chronic otorrhea. Although the disease can be treated surgically, the recurrence rate is high. This study explored whether autologous oral mucosal epithelial cell sheets could be used as a novel therapy for ear canal atresia. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.031DOI Listing

Acid-induced demineralisation of human enamel as a function of time and pH observed using X-ray and polarised light imaging.

Acta Biomater 2020 May 11. Epub 2020 May 11.

School of Dentistry, Institute of Clinical Sciences, University of Birmingham, 5 Mill Pool Way, Edgbaston, Birmingham B5 7EG, UK.

Acid-induced enamel demineralisation affects many individuals either by exposure to acidic diets, acidic gas pollution (dental erosion) or to dental plaque acids (dental caries). This study aimed to develop in situ X-ray and light imaging methods to determine progression of enamel demineralisation and the dynamic relationship between acid pH and mineral density. Hourly digital microradiograph time-lapse sequences showed the depth of enamel demineralisation in 500 µm thick sections progressed with time from the surface towards the dentine following a power-law function, which was 21% faster than the lateral demineralisation progression after exposure for 85 h to lactic acid (10%, pH 2. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.045DOI Listing

Hybrid Membrane Camouflaged Copper Sulfide Nanoparticles for Photothermal-chemotherapy of Hepatocellular Carcinoma: Consistent with the observations so far, CuS-SF@CMV+NIR significantly reduced the number of nidi on the liver compared to CuS@CM+NIR (p=0.0337) and CuS@CMV+NIR (p=0.0384) (Figure S15A top panel, S15B). In addition, fewer macroscopic lesions were seen in the liver, lung and spleens of the CuS-SF@CMV+NIR group compared to the other treatment groups (Figure S15A), and the former also had the smallest spleens among all groups (Figure S15C). Histologically, the tumors of the CuS@CM+NIR, CuS@CMV+NIR groups exhibited a more regular structure, while the remaining groups showed severe cellular lesions and tumor atypia (). In addition, CuS-SF@CMV+NIR was able to restore the morphology of the hepatocytes in the tumor masses to that in normal liver. Furthermore, the tumor tissues in the PBS, PBS+NIR, CuS@CM, CuS@CMV and CuS-SF@CMV groups showed high levels of PCNA, which was decreased in the free sofarenib (SF), CuS@CM+NIR and CuS@CMV+NIR groups, and was lowest in the CuS-SF@CMV+NIR group (Figure S16). Finally, the combination of CuS-SF@CMV and NIR significantly reduced the levels of p-AKT (Figure S16 middle panel) and p-ERK (Figure S16 bottom panel) in the tumors. Finally, to further confirm the tumor targeting of CuS-SF@CMV, we performed the biodistribution of various nanoparticles independently in the tumors and major organs of the sacrificed mice for the Cu measurement using ICP-AES (Figure S17). Comparing to CuS, CuS-SF@CMV exhibited significantly higher accumulation in the tumor and lower accumulation in liver and spleen. The accumulation in major organs did not show significant difference from CuS@CM, demonstrating that the hybrid cell membrane coating endows nanoparticles with the targeting ability. Taking together all the findings, the CuS-SF@CMV NPs selectively accumulated in the tumors, and significantly inhibited tumor growth due to NIR-responsive synergistic photo-thermal and chemotherapy. Mechanistically, it decreased the proliferation of tumor cells by inhibiting the PI3K/AKT and Ras/Raf/MEK/ERK signaling pathways, which likely resulted in less neo-angiogenesis as well. Thus, CuS-SF@CMV NPs are an ideal platform for targeted tumor ablation through rapid hyperthermia-induced cancer cell death and a more sustained inhibition via sofarenib and anti-VEGFR antibody, with minimal damage to the normal tissues.

Acta Biomater 2020 May 17. Epub 2020 May 17.

Department of Hepatobiliary and Pancreatic Surgery, the First Hospital, Jilin University, 71 Xinmin Street, Changchun 130021, China. Electronic address:

Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related deaths worldwide. Biomimetic nanoparticles (NPs) coated with cell membranes show enhanced biocompatibility and specificity for homotypic cells, and have gained considerable attention for targeted anti-tumor therapy. We constructed cancer cell-macrophage hybrid membrane-coated near infrared (NIR)-responsive hollow copper sulfide nanoparticles encapsulating sorafenib and surface modified with anti-VEGFR (CuS-SF@CMV NPs). Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.046DOI Listing

Late Endothelial progenitor cell-capture stents with CD146 antibody and nanostructure reduce in-stent restenosis and thrombosis.

Acta Biomater 2020 May 17. Epub 2020 May 17.

Center for Biomaterials, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea; Department of Biomedical Engineering, Korea University of Science and Technology, Daejeon, 34113, Republic of Korea. Electronic address:

The restoration of damaged endothelium is promising to reduce side effects, including restenosis and thrombosis, in the stent treatment for vascular diseases. Current technologies based on drug delivery for these complications still do not satisfy patients due to invariant recurrence rate. Recently, even if one approach was applied to clinical trial to develop the firstly commercialized stent employing circulating endothelial progenitor cells (EPCs) in blood vessels, it resulted in failure in clinical test. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.011DOI Listing

T cells modulate IL-4 expression by eosinophil recruitment within decellularized scaffolds to repair nerve defects.

Acta Biomater 2020 May 17. Epub 2020 May 17.

Division of Plastic and Reconstructive Surgery, Department of Surgery, Washington University School of Medicine, St. Louis, MO, 63110, U.S.A.. Electronic address:

Decellularized nerve, or acellular nerve allografts (ANAs), are an increasingly used alternative to nerve autografts to repair nerve gaps to facilitate regeneration. The adaptive immune system, specifically T cells, plays a role in promoting regeneration upon these ANA scaffolds. However, how T cells promote regeneration across ANAs is not clear. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.009DOI Listing

A di-self-crosslinking hyaluronan-based hydrogel combined with type I collagen to construct a biomimetic injectable cartilage-filling scaffold.

Acta Biomater 2020 May 17. Epub 2020 May 17.

National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Road, Chengdu, Sichuan, 610064, P. R. China.

Injectable hydrogels have attracted increasing attention because of convenient clinical operation, non-invasive surgical procedure and seamless filling of irregular defects. Here, injectable di-self-crosslinking HSMSSA hydrogel was formed via fast thiol/maleimide click chemistry reaction and thiol oxidation reaction as primary and secondary self-crosslinking network, respectively. Molecular weight and precursor concentration significantly affected physichemical properties and biological functions of hydrogels. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.007DOI Listing
May 2020
6.025 Impact Factor

Fluorescent Nanoparticles with Ultralow Chromophore Loading for Long-Term Tumor-Targeted Imaging.

Acta Biomater 2020 May 17. Epub 2020 May 17.

School of Chemistry and Life Science, Advanced Institute of Materials Science, Changchun University of Technology, 2055 Yanan Street, Changchun, Jilin 130012, P. R. China. Electronic address:

Recently, organic dyes with aggregation-induced emission (AIE) have attracted much attention in bioimaging and diagnostics. Relatively, the application of traditional dyes has diminished because of aggregation-caused quenching (ACQ). In this work, we compare the imaging ability of nanoparticle formulations of these two kinds of dyes. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.013DOI Listing

Monitoring matrix remodelling in the cellular microenvironment using microrheology for complex cellular systems.

Acta Biomater 2020 May 17. Epub 2020 May 17.

Institute of Mechanical Process Engineering and Mechanics, Applied Mechanics Group, Karlsruhe Institute of Technology, 76137 Karlsruhe, Germany.

Multiple particle tracking (MPT) microrheology was employed for monitoring the development of extracellular matrix (ECM) mechanical properties in the direct microenvironment of living cells. A customized setup enabled us to overcome current limitations: (i) Continuous measurements were enabled using a cell culture chamber, with this, matrix remodelling by fibroblasts in the heterogeneous environment of macroporous scaffolds was monitored continuously. (ii) Employing tracer laden porous scaffolds for seeding human mesenchymal stem cells (hMSCs), we followed conventional differentiation protocols. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.053DOI Listing

Assessing and improving the biocompatibility of microfluidic artificial lungs.

Acta Biomater 2020 May 17. Epub 2020 May 17.

VA Ann Arbor Healthcare System, 2215 Fuller Road, Ann Arbor, MI, USA, 48105; University of Michigan, 1150 W. Medical Center Drive, Ann Arbor, MI, USA, 48109.

Microfluidic artificial lungs (µALs) have the potential to improve the treatment and quality of life for patients with acute or chronic lung injury. In order to realize the full potential of this technology (including as a destination therapy), the biocompatibility of these devices needs to be improved to produce long-lasting devices that are safe for patient use with minimal or no systemic anticoagulation. Many studies exist which probe coagulation and thrombosis on polydimethyl siloxane (PDMS) surfaces, and many strategies have been explored to improve surface biocompatibility. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.008DOI Listing

Design of Light/ROS Cascade-Responsive Tumor-Recognizing Nanotheranostics for Spatiotemporally Controlled Drug Release in Locoregional Photo-Chemotherapy.

Acta Biomater 2020 May 17. Epub 2020 May 17.

Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361005, PR China. Electronic address:

Carrier-free nanotheranostics with high drug loading and no carrier-related toxicity are highly promising cancer therapy agents. However, the limited tumor accumulation and poorly controlled drug release of these nanotheranostics continue to be major challenges that restrict clinical applications. In this study, we develop a tumor-recognizing carrier-free nanotheranostic with light/reactive oxygen species (ROS) cascade-responsiveness for spatiotemporally selective photo-chemotherapy. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.052DOI Listing

Peptides from diatoms and grasses harness phosphate ion binding to silica to help regulate biomaterial structure.

Acta Biomater 2020 May 17. Epub 2020 May 17.

Department of Chemistry, Bar-Ilan University, Ramat Gan 5290002, Israel. Electronic address:

Many life forms generate intricate submicron biosilica structures with various important biological functions. The formation of such structures, from the silicic acid in the waters and in the soil, is thought to be regulated by unique proteins with high repeats of specific amino acids and unusual sidechain modifications. Some silicifying proteins are characterized by high prevalence of basic amino acids in their primary structures. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.006DOI Listing

Electrostatic Driven Transport Enhances Penetration of Positively Charged Peptide Surfaces through Tumor Extracellular Matrix.

Acta Biomater 2020 May 16. Epub 2020 May 16.

Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy, The University of Texas at Austin, USA. Electronic address:

Drug carriers achieve poor and heterogeneous distribution within solid tumors due to limited transport through the tumor extracellular matrix (ECM). The tumor ECM forms a net negatively charged network that interacts with and hinders the transport of molecules in part due to electrostatic interactions. Traditionally, the surfaces of drug delivery systems are passivated to minimize these interactions, but the mechanism of how charge interactions impact transport and penetration within the tumor microenvironment is not well understood. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.051DOI Listing

Tracking tendon fibers to their insertion - a 3D analysis of the Achilles tendon enthesis in mice.

Acta Biomater 2020 May 16. Epub 2020 May 16.

Institut für Zoologie und Evolutionsforschung, Friedrich-Schiller-Universität Jena, Erbertstraße 1, 07743 Jena, Germany.

Tendon insertions to bone are heavily loaded transitions between soft and hard tissues. The fiber courses in the tendon have profound effects on the distribution of stress along and across the insertion. We tracked fibers of the Achilles tendon in mice in micro-computed tomographies and extracted virtual transversal sections. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.001DOI Listing

Early Changes in Cartilage Pericellular Matrix Micromechanobiology Portend the Onset of Post-Traumatic Osteoarthritis.

Acta Biomater 2020 May 16. Epub 2020 May 16.

School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA 19104, United States. Electronic address:

The pericellular matrix (PCM) of cartilage is a structurally distinctive microdomain surrounding each chondrocyte, and is pivotal for cell homeostasis and cell-matrix interactions in healthy tissue. This study queried if the PCM is the initiation point for disease or a casualty of more widespread matrix degeneration. To address this question, we queried the mechanical properties of the PCM and chondrocyte mechanoresponsivity with the development of post-traumatic osteoarthritis (PTOA). Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.005DOI Listing

Biocompatibility and Bioactivity of an FGF-Loaded Microsphere-Based Bilayer Delivery System.

Acta Biomater 2020 May 16. Epub 2020 May 16.

Department of Orthopaedic Surgery, University of Pennsylvania, McKay Orthopaedic Research Laboratory, 36th Street and Hamilton Walk, 374 Stemmler Hall, Philadelphia, PA 19104-6081, USA; Translational Musculoskeletal Research Center, Philadelphia VA Medical Center, 3900 Woodland Avenue, Building 21, Room A200, Philadelphia, PA 19104, USA. Electronic address:

Many drug delivery systems rely on degradation or dissolution of the carrier material to regulate release. In cases where mechanical support is required during regeneration, this necessitates composite systems in which the mechanics of the implant are decoupled from the drug release profile. To address this need, we developed a system in which microspheres (MS) were sequestered in a defined location between two nanofibrous layers. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.048DOI Listing

Proteome analysis of secretions from human monocyte-derived macrophages post-exposure to biomaterials and the effect of secretions on cardiac fibroblast fibrotic character.

Acta Biomater 2020 May 16. Epub 2020 May 16.

Faculty of Dentistry, University of Toronto, Toronto, Ontario M5G 1G6, Canada; Translational Biology and Engineering Program and Ted Rogers Centre for Heart Research, Toronto, Ontario M5G 1M1, Canada; Institute of Biomaterial and Biomedical Engineering, Toronto, Ontario M5S 3G9, Canada. Electronic address:

The use of exogenous biomolecules (BM) for the purpose of repairing and regenerating damaged cardiac tissue can yield serious side effects if used for prolonged periods. As well, such strategies can be cost prohibitive depending on the regiment and period of time applied. Alternatively, autologous monocytes/monocyte-derived macrophages (MDM) can provide a viable path towards generating an endogenous source of stimulatory BM. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.042DOI Listing

Microtensile properties and failure mechanisms of cortical bone at the lamellar level.

Acta Biomater 2020 May 16. Epub 2020 May 16.

Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Mechanics of Materials and Nanostructures, Feuerwerkerstrasse 39, CH-3602 Thun, Switzerland. Electronic address:

Bone features a remarkable combination of toughness and strength which originates from its complex hierarchical structure and motivates its investigation on multiple length scales. Here, in situ microtensile experiments were performed on dry ovine osteonal bone for the first time at the length scale of a single lamella. The micromechanical response was brittle and revealed larger ultimate tensile strength compared to the macroscale (factor of 2. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.030DOI Listing

Effects of shell thickness of hollow poly(lactic acid) nanoparticles on sustained drug delivery for pharmacological treatment of glaucoma.

Acta Biomater 2020 May 16. Epub 2020 May 16.

Graduate Institute of Biomedical Engineering, Chang Gung University, Taoyuan 33302, Taiwan, ROC; Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou, Taoyuan 33305, Taiwan, ROC; Department of Materials Engineering, Ming Chi University of Technology, New Taipei City 24301, Taiwan, ROC. Electronic address:

Structural designing of carriers with extended drug release profiles is critically important for achieving long-acting drug delivery systems toward efficient managements of chronic diseases. Here, we present a strategy to exploit the effects of the shell thickness of hollow poly(lactic acid) nanoparticles (HPLA NPs) in sustained glaucoma therapy. Formulations based on pilocarpine-loaded HPLA NPs with tailorable shell thickness ranging from 10 to 100 nm were shown to be highly compatible with human lens epithelial cells in vitro and with rabbit eyes in vivo. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.055DOI Listing

3D-Printed Scaffolds with Carbon Nanotubes for Bone Tissue Engineering: Fast and Homogeneous One-Step Functionalization.

Acta Biomater 2020 May 16. Epub 2020 May 16.

Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55905, USA.; Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN 55905, USA.. Electronic address:

Three-dimensional (3D) printing is a promising technology for tissue engineering. However, 3D-printing methods are limited in their ability to produce desired microscale features or electrochemical properties in support of robust cell adhesion, proliferation, and differentiation. This study addresses this deficiency by proposing an integrated, one-step, method to increase the cytocompatibility of 3D-printed scaffolds through functionalization leveraging conductive carbon nanotubes (CNTs). Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.047DOI Listing

Deployable, liquid crystal elastomer-based intracortical probes.

Acta Biomater 2020 May 17. Epub 2020 May 17.

Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA.

Intracortical microelectrode arrays (MEAs) are currently limited in their chronic functionality due partially to the foreign body response (FBR) that develops in regions immediately surrounding the implant (typically within 50-100 µm). Mechanically flexible, polymer-based substrates have recently been explored for MEAs as a way of minimizing the FBR caused by the chronic implantation. Nonetheless, the FBR degrades the ability of the device to record neural activity. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.032DOI Listing

INTRA-MYOCARDIAL ALGINATE HYDROGEL INJECTION ACTS AS A LEFT VENTRICULAR MID-WALL CONSTRAINT IN SWINE.

Acta Biomater 2020 May 16. Epub 2020 May 16.

Department of Surgery, University of California at San Francisco, San Francisco, California, USA. Electronic address:

Despite positive initial outcomes emerging from preclinical and early clinical investigation of alginate hydrogel injection therapy as a treatment for heart failure, the lack of knowledge about the mechanism of action remains a major shortcoming that limits the efficacy of treatment design. To identify the mechanism of action, we examined previously unobtainable measurements of cardiac function from in vivo, ex vivo, and in silico states of clinically relevant heart failure (HF) in large animals. High-resolution ex vivo magnetic resonance imaging and histological data were used along with state-of-the-art subject-specific computational model simulations. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.033DOI Listing

Magnetically responsive peptide coacervates for dual hyperthermia and chemotherapy treatments of liver cancer.

Acta Biomater 2020 May 15. Epub 2020 May 15.

Biological and Biomimetic Materials Laboratory, Centre for Biomimetic Sensor Science, School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 637553; School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551. Electronic address:

Liver cancer is an aggressive malignancy associated with high levels of mortality and morbidity. Doxorubicin (Dox) is often used to slow down liver cancer progression; however its efficacy is limited, and its severe side effects prevent its routine use at therapeutic concentrations. We present a biomimetic peptide that coacervates into micro-droplets, within which both Dox and magnetic nanoparticles (MNPs) can be sequestered. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.024DOI Listing

Perinatal tissues and cells in tissue engineering and regenerative medicine.

Acta Biomater 2020 May 14. Epub 2020 May 14.

CICECO, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal. Electronic address:

Perinatal tissues are an abundant source of human extracellular matrix proteins, growth factors and stem cells with proved potential use in a wide range of therapeutic applications. Due to their placental origin, these tissues possess unique biological properties, including being angiogenic, anti-inflammatory, anti-fibrotic, anti-microbial and immune privileged. Additionally, as a temporary organ, placenta is usually discarded as a medical waste, thus providing an easily available, cost effective, 'unlimited' and ethical source of raw materials. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.035DOI Listing

Bispecific Antibody (HER2 × mPEG) Enhances Anti-Cancer Effects by Precise Targeting and Accumulation of mPEGylated Liposomes.

Acta Biomater 2020 May 14. Epub 2020 May 14.

Graduate Institute of Medicine College of Medicine Kaohsiung Medical University, Kaohsiung, Taiwan; Drug Development and Value Creation Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan. Electronic address:

Targeted antibodies and methoxy-PEGylated nanocarriers have gradually become a mainstream of cancer therapy. To increase the anti-cancer effects of targeted antibodies combined with mPEGylated liposomes (mPEG-liposomes), we describe a bispecific antibody in which an anti-methoxy-polyethylene glycol scFv (αmPEG scFv) was fused to the C-terminus of an anti-HER2 (αHER2) antibody to generate a HER2 × mPEG BsAb that retained the original efficacy of a targeted antibody while actively attracting mPEG-liposomes to accumulate at tumor sites. HER2 × mPEG BsAb can simultaneously bind to HER2-high expressing MCF7/HER2 tumor cells and mPEG molecules on mPEG-liposomal doxorubicin (Lipo-Dox). Read More

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http://dx.doi.org/10.1016/j.actbio.2020.04.029DOI Listing
May 2020
6.025 Impact Factor