705,547 results match your criteria Acta Scientiae Veterinariae[Journal]

A water-soluble manganese(II) octanediaoate/phenanthroline complex acts as an antioxidant and attenuates alpha-synuclein toxicity.

Biochim Biophys Acta Mol Basis Dis 2022 Jun 28:166475. Epub 2022 Jun 28.

Departamento de Bioquímica, Instituto de Química, Centro de Tecnologia, Cidade Universitária, Universidade Federal do Rio de Janeiro, Brazil; Rede de Micrologia RJ-FAPERJ, Brazil. Electronic address:

The overproduction of reactive oxygen species (ROS) induces oxidative stress, a well-known process associated with aging and several human pathologies, such as cancer and neurodegenerative diseases. A large number of synthetic compounds have been described as antioxidant enzyme mimics, capable of eliminating ROS and/or reducing oxidative damage. In this study, we investigated the antioxidant activity of a water-soluble 1,10-phenantroline-octanediaoate Mn-complex on cells under oxidative stress, and assessed its capacity to attenuate alpha-synuclein (aSyn) toxicity and aggregation, a process associated with increased oxidative stress. Read More

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Amyloid-β aggregates induced by β-cholesteryl glucose-embedded liposomes.

Biochim Biophys Acta Proteins Proteom 2022 Jun 28:140816. Epub 2022 Jun 28.

Graduate School of Environment and Life Science, Okayama University, 3-1-1 Tsushimanaka, kita-kku, Okayama 700-8530, Japan.

Senile plaques that is characterized as an amyloid deposition found in Alzheimer's disease are composed primarily of fibrils of an aggregated peptide, amyloid β (Aβ). The ability to monitor senile plaque formation on a neuronal membrane under physiological conditions provides an attractive model. In this study, the growth behavior of amyloid Aβ fibrils in the presence of liposomes incorporating β-cholesteryl-D-glucoside (β-CG) was examined using total internal reflection fluorescence microscopy, transmittance electron microscopy, and other spectroscopic methods. Read More

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Novel insights into noncanonical open reading frames in cancer.

Biochim Biophys Acta Rev Cancer 2022 Jun 28:188755. Epub 2022 Jun 28.

Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, Hunan, China; The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan 410008, China. Electronic address:

With technological advances, previously neglected noncanonical open reading frames (nORFs) are drawing ever-increasing attention. However, the translation potential of numerous putative nORFs remains elusive, and the functions of noncanonical peptides have not been systemically summarized. Moreover, the relationship between noncanonical peptides and their counterpart protein or RNA products remains elusive and the clinical implementation of noncanonical peptides has not been explored. Read More

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YAP and TAZ: Monocorial and bicorial transcriptional co-activators in human cancers.

Biochim Biophys Acta Rev Cancer 2022 Jun 28:188756. Epub 2022 Jun 28.

Translational Oncology Research Unit, Department of Research, Diagnosis and Innovative Technologies, IRCCS Regina Elena National Cancer Institute, Rome, Italy. Electronic address:

The transcriptional regulators YAP and TAZ are involved in numerous physiological processes including organ development, growth, immunity and tissue regeneration. YAP and TAZ dysregulation also contribute to tumorigenesis, thereby making them attractive cancer therapeutic targets. Arbitrarily, YAP and TAZ are often considered as a single protein, and are referred to as YAP/TAZ in most studies. Read More

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An injectable co-assembled hydrogel blocks reactive oxygen species and inflammation cycle resisting myocardial ischemia-reperfusion injury.

Acta Biomater 2022 Jun 28. Epub 2022 Jun 28.

Guangdong Provincial Biomedical Engineering Technology Research Center for Cardiovascular Disease, Department of Cardiology and Laboratory of Heart Center, Sino-Japanese Cooperation Platform for Translational Research in Heart Failure, Guangdong Provincial Key Laboratory of Shock and Microcirculation, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, China. Electronic address:

The overproduction of reactive oxygen species (ROS) and burst of inflammation following cardiac ischemia-reperfusion (I/R) are the leading causes of cardiomyocyte injury. Monotherapeutic strategies designed to enhance anti-inflammatory or anti-ROS activity explicitly for treating I/R injury have demonstrated limited success because of the complex mechanisms of ROS production and induction of inflammation. Intense oxidative stress leads to sustained injury, necrosis, and apoptosis of cardiomyocytes. Read More

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TRIM22 negatively regulates MHC-II expression.

Biochim Biophys Acta Mol Cell Res 2022 Jun 28:119318. Epub 2022 Jun 28.

Department of Biochemistry, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Kita 15, Nishi 7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan. Electronic address:

The development of cancer treatment has recently achieved a remarkable breakthrough, and checkpoint blockade immunotherapy has received much attention. To enhance the therapeutic efficacy of checkpoint blockade immunotherapy, recent studies have revealed the importance of activation of CD4 T cells via an increase in major histocompatibility complex (MHC) class II molecules in cancer cells. Here, we demonstrate that tripartite motif-containing (TRIM) 22, negatively regulates MHC-II expression. Read More

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Readers accurately monitor their incidental word learning success during silent reading.

Acta Psychol (Amst) 2022 Jun 27;228:103653. Epub 2022 Jun 27.

Department of Psychological Sciences, P.O. Box 5190, Kent State University, Kent, OH 44242-0001, USA. Electronic address:

College-age adults who are skilled at reading use sentence context and morphemic information to learn definitions for new words they encounter in print (e.g., Tong et al. Read More

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Negative urgency is related to impaired response inhibition during threatening conditions.

Acta Psychol (Amst) 2022 Jun 27;228:103648. Epub 2022 Jun 27.

Centre for Mental Health, Swinburne University of Technology, Australia.

While it has been argued that impulsivity and inhibition are unrelated, previous evidence suggests that the relationship between the two can only be seen when their characteristics are closely matched. The negative urgency subscale of the UPPS-P describes impulsive action during negative affect. This was predicted to correlate more strongly with stop-signal reaction-time (SSRT) during threatening conditions than non-threatening conditions. Read More

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microRNA-25-3p suppresses osteogenic differentiation of BMSCs in patients with osteoporosis by targeting ITGB3.

Acta Histochem 2022 Jun 28;124(6):151926. Epub 2022 Jun 28.

Department of Orthopedics, the First Hospital of Nanchang, Nanchang, Jiangxi 330008, PR China.

This study was conducted to investigate the impact of the microRNA (miR)-25-3p/ITGB3 axis on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) from patients with osteoporosis (OP). BMSCs isolated from the bone marrow of healthy controls and OP patients were identified by flow cytometry, in which ITGB3 mRNA and miR-25-3p expression was detected by RT-qPCR and ITGB3, Runx2, OPN, ALP, and OSX protein expression by western blot. The binding between ITGB3 and miR-25-3p was assessed by dual-luciferase reporter gene and Ago2-RIP assays. Read More

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Fluorescence recovery based on synergetic effect for ALP detection.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Jun 22;280:121550. Epub 2022 Jun 22.

CAS Key Laboratory of Quantitative Engineering Biology, Guangdong Provincial Key Laboratory of Synthetic Genomics and Shenzhen Key Laboratory of Synthetic Genomics. Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China. Electronic address:

Alkaline phosphatase (ALP) is an important biomarker associated with diabetes, liver dysfunction, bone diseases, and breast cancer. Here we developed a method based on synergetic fluorescence recovery for the sensitive detection of ALP. Cadmium-zinc-selenium (CdZnSe) quantum dots (QDs) were prepared by one-pot water bath method without any complicated and rigorous conditions. Read More

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Nonlinear optical properties and optimization strategies of D-π-A type phenylamine derivatives in the near-infrared region.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Jun 22;280:121539. Epub 2022 Jun 22.

School of Physical Science and Technology, Soochow University, Soochow 215006, Jiangsu, China; Department of Physics, Harbin Institute of Technology, Harbin 150006, Heilongjiang, China. Electronic address:

Modifying simple molecular structures to significantly improve nonlinear optical (NLO) performance is a primary prerequisite for scientific research. Based on the four phenylamine derivatives reported in previous studies, we designed four organic nonlinear molecules by changing the acceptor group and π-linker. (Time-dependent) density functional theory (DFT/TD-DFT) was performed on molecular geometry optimization, the contribution of π electrons to the bond order, linear and two-photon absorption (TPA) spectra, the intra-molecular charge transfer matrix (CTM), and NLO coefficients. Read More

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Exploring the influence of intermolecular hydrogen bonding on the fluorescence properties of HQCT and HQPH fluorescent chemosensors.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Jun 20;280:121537. Epub 2022 Jun 20.

School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, PR China.

Recently, two trace water detection probes, 8-hydroxyquinoline-2-carboxaldehyde thiosemicarbazone(HQCT) and 8-hydroxyquinoline-2-carboxaldehyde (pyridine-2-carbonyl)-hydrazine(HQPH) have been successfully designed in the experiment. The original intramolecular proton transfer can be prevented by the water molecules, leading to fluorescence quenching. In order to investigate the fluorescence quenching mechanism, the effect of water molecules on the excited state proton transfer process will be studied in detail. Read More

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Insights into the photophysical properties of 2-(2'-hydroxyphenyl) benzazoles derivatives: Application of ESIPT mechanism on UV absorbers.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Jun 27;280:121559. Epub 2022 Jun 27.

College of Science, Northeast Forestry University, Harbin 150040, China. Electronic address:

In this present work, four novel molecules (BPN, BPNS, BPS, and BPSN), possessing excited-state intramolecular proton transfer (ESIPT) characteristics, were designed to quantify the impacts of substituent effects on their photophysical properties. By exploring the primary geometrical parameters concerning hydrogen bonds, it should be noticed that the intramolecular hydrogen bonds (IHBs) of the studied molecules have been strengthened at S state. Infrared vibrational spectra analysis illustrates that adding electron-donating group thiophene to the proton donor side can weaken the IHBs in comparison to the electron-withdrawing group pyridine. Read More

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The association between myofascial orofacial pain with and without referral and widespread pain.

Acta Odontol Scand 2022 Jul 1:1-6. Epub 2022 Jul 1.

Department of Odontology/Clinical Oral Physiology, Faculty of Medicine, University of Umeå, Umeå, Sweden.

Objectives: Pain referral on palpation has been suggested to be a clinical sign of central sensitization potentially associated with widespread pain conditions. Our aim was to evaluate if myofascial pain with referral is a better predictor for widespread pain when compared to no pain or local myofascial pain.

Materials And Methods: Individuals at the Public Dental services in Västerbotten, Sweden, were randomly invited based on their answers to three screening questions for temporomandibular disorders (TMD). Read More

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Malignant odontogenic tumors: epigenetics in disease and therapy.

Acta Odontol Scand 2022 Jul 1:1-5. Epub 2022 Jul 1.

PG & Research Department of Biotechnology and Microbiology, National College (Autonomous), Tiruchirappalli, India.

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Dual task effect on upper and lower extremity skills in different stages of Parkinson's disease.

Acta Neurol Belg 2022 Jul 1. Epub 2022 Jul 1.

Faculty of Physical Therapy and Rehabilitation, Hacettepe University, Ankara, Turkey.

Background And Purpose: Loss of automaticity and deteriorated executive function give rise to dual task deficits in Parkinson's disease (PD). This study aimed to compare single task and dual task upper and lower extremity skills in people with PD (PwPD) at different stages of PD and to examine the dual task effect (DTE) on upper and lower extremity skills in PwPD at different stages of PD. The second aim was to investigate the relationship between the DTE and the quality of life in PwPD. Read More

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Nitric oxide detection using catalytic properties of CuCo-PTC metal organic framework.

Mikrochim Acta 2022 Jul 1;189(7):263. Epub 2022 Jul 1.

Green Catalysis Center, and College of Chemistry, Zhengzhou University, Zhengzhou, 450001, China.

As a vital gaseous signal molecule involved in various physiological and pathological processes, nitric oxide (NO) has attracted extensive attention in the last few decades. In this work, a copper and cobalt element-doped, biphenyl-(3, 4', 5)-tricarboxylic acid (HPTC)-synthesized metal organic framework (CuCo-PTC MOF) composite with catalytic ability was synthesized by solvothermal method. The material can catalyse the oxidation of o-phenylenediamine (OPD) groups by hydrogen peroxide (HO) to form fluorophores (OPDox) with yellow fluorescence emission and greatly improves its reaction rate. Read More

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Photoelectrochemical sandwich immunoassay of brain glycogen phosphorylase based on methyl orange-sensitized TiO nanorods.

Mikrochim Acta 2022 Jul 1;189(7):265. Epub 2022 Jul 1.

Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, China.

The photoelectrochemical immunoassay of glycogen phosphorylase BB (GPBB) was studied. A methyl orange/TiO nanorod heterojunction was constructed on a fluorine-doped tin oxide electrode by hydrothermal synthesis, calcination, and chemical adsorption. A sandwich immune structure consisting of GPBB as the first antibody, GPBB, and a [email protected] silica-ascorbic acid (AA)-GPBB as secondary antibody composite was constructed on each of the selected well surfaces of a 96-well microplate. Read More

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Fourth-generation bypass and flow reversal to treat a symptomatic giant dolichoectatic basilar trunk aneurysm.

Acta Neurochir (Wien) 2022 Jul 1. Epub 2022 Jul 1.

Department of Neurosurgery, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, 350 W Thomas Rd, Phoenix, AZ, 85013, USA.

Background: Giant dolichoectatic basilar trunk aneurysms have an unfavorable natural history and are associated with high morbidity, but their neurosurgical treatment is complex and challenging.

Methods: Flow reversal reconstruction with fourth-generation bypass and proximal vertebral artery clip occlusion is performed via orbitozygomatic craniotomy with the Kawase approach under rapid ventricular pacing.

Conclusion: Fourth-generation bypass is an innovative, technically challenging, and clinically effective tool in the treatment armamentarium for giant dolichoectatic basilar trunk aneurysms. Read More

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The "Deep Subarcuate Fossa" sign and three types of anomalous subarcuate loops encountered during vestibular schwannoma removal.

Acta Neurochir (Wien) 2022 Jul 1. Epub 2022 Jul 1.

Department of Neurosurgery, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Background: An anomalous subarcuate loop (SL) of the anteroinferior cerebellar artery (AICA) is a rare anatomic variation, which increases the complexity and risk of vestibular schwannoma (VS) removal. However, preoperative diagnosis of this anomaly remains difficult. The aim of this study was to report three types of anomalous SLs encountered during VS removal and to describe the "Deep Subarcuate Fossa (SF)" sign and its significance in the diagnosis and treatment of an osseous-penetrating SL. Read More

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pH-activated DNA nanomachine for miRNA-21 imaging to accurately identify cancer cell.

Mikrochim Acta 2022 Jul 1;189(7):266. Epub 2022 Jul 1.

Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, China.

MicroRNA (miRNA) imaging has been employed to distinguish cancer cells from normal cells by exploiting the overexpression of miRNA in cancer. Inspired by the acidic extracellular tumor microenvironment, we designed a pH-activated DNA nanomachine to enable the specific detection of cancer cells using miRNA imaging. The DNA nanomachine was engineered by assembling two hairpins (Y1 and Y2) onto the surface of a ZIF-8 metal-organic framework (MOF), which decomposed under acidic conditions to release the adsorbed DNA hairpin molecules in situ. Read More

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Coupled electrochemiluminescent and resonance energy transfer determination of microRNA-141 using functionalized Mxene composite.

Mikrochim Acta 2022 Jul 1;189(7):264. Epub 2022 Jul 1.

Key Laboratory of Structure and Functional Regulation of Hybrid Materials (Ministry of Education), School of Chemistry and Chemical Engineering, Anhui University, Hefei, 230601, Anhui, China.

The electrochemiluminescence and resonance energy transfer (ECL-RET) method was adopted to detect miRNAs, in which the two-dimensional TiC Mxenes with high surface area modified with CdS:W nanocrystals (CdS:W NCs) were used as ECL signal emitter. Mxenes with a specific surface area of 5.2755 m/g carried more emitters and promote ECL intensity. Read More

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Structural and biophysical studies of new L-asparaginase variants: lessons from random mutagenesis of the prototypic Escherichia coli Ntn-amidohydrolase.

Acta Crystallogr D Struct Biol 2022 Jul 28;78(Pt 7):911-926. Epub 2022 Jun 28.

Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.

This work reports the results of random mutagenesis of the Escherichia coli class 2 L-asparaginase EcAIII belonging to the Ntn-hydrolase family. New variants of EcAIII were studied using structural, biophysical and bioinformatic methods. Activity tests revealed that the L-asparaginase activity is abolished in all analyzed mutants with the absence of Arg207, but some of them retained the ability to undergo the autoproteolytic maturation process. Read More

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Development of CryoVR, a virtual reality training system for hands-on cryoEM operations.

Acta Crystallogr D Struct Biol 2022 Jul 27;78(Pt 7):903-910. Epub 2022 Jun 27.

Computer Graphics Technology, Purdue University, Knoy Hall Room 363, 401 North Grant Street, West Lafayette, IN 47907, USA.

Cryogenic electron microscopy (cryoEM) has emerged as a revolutionary method for solving high-resolution structures and studying the dynamics of macromolecular complexes and viruses in near-native states. However, the availability of the equipment, and the time and cost needed for training, severely limit the opportunities for training. To solve these problems, a virtual reality-based training system, CryoVR, has been developed to prepare trainees before operating real-world cryoEM equipment. Read More

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AutoPX: a new software package to process X-ray diffraction data from biomacromolecular crystals.

Acta Crystallogr D Struct Biol 2022 Jul 27;78(Pt 7):890-902. Epub 2022 Jun 27.

School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.

A new software package, autoPX, for processing X-ray diffraction data from biomacromolecular crystals is reported. This processing software package is designed on the basis of novel methods such as the location of diffraction spots by an improved Canny operator, indexing by a modified Fourier transform, a novel definition of mosaicity that expresses the dispersion state of reciprocal diffraction spots, and the correction of predicted diffraction spot coordinates by homography transform. New programming of some traditional algorithms necessary for integration and scaling is also included. Read More

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Microsecond melting and revitrification of cryo samples: protein structure and beam-induced motion.

Acta Crystallogr D Struct Biol 2022 Jul 14;78(Pt 7):883-889. Epub 2022 Jun 14.

Laboratory of Molecular Nanodynamics, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

A novel approach to time-resolved cryo-electron microscopy (cryo-EM) has recently been introduced that involves melting a cryo sample with a laser beam to allow protein dynamics to briefly occur in the liquid, before trapping the particles in their transient configurations by rapidly revitrifying the sample. With a time resolution of just a few microseconds, this approach is notably fast enough to study the domain motions that are typically associated with the activity of proteins but which have previously remained inaccessible. Here, crucial details are added to the characterization of the method. Read More

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Biochemical and structural insights into an unusual, alkali-metal-independent S-adenosyl-L-homocysteine hydrolase from Synechocystis sp. PCC 6803.

Acta Crystallogr D Struct Biol 2022 Jul 14;78(Pt 7):865-882. Epub 2022 Jun 14.

Department of Structural Biology of Prokaryotic Organisms, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.

The mesophilic cyanobacterium Synechocystis sp. PCC 6803 encodes an S-adenosyl-L-homocysteine hydrolase (SAHase) of archaeal origin in its genome. SAHases are essential enzymes involved in the regulation of cellular S-adenosyl-L-methionine (SAM)-dependent methylation reactions. Read More

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Structural basis of ligand recognition and transport by Sfh2, a yeast phosphatidylinositol transfer protein of the Sec14 superfamily.

Acta Crystallogr D Struct Biol 2022 Jul 14;78(Pt 7):853-864. Epub 2022 Jun 14.

College of Pharmacy, Chonnam National University, Gwangju 61186, Republic of Korea.

Sec14-like phosphatidylinositol transfer proteins (PITPs) are involved in lipid metabolism and phosphatidylinositol 4-phosphate signaling by transporting phosphatidylinositol (PI) and a secondary ligand between the organellar membranes in eukaryotes. Yeast Sfh2 is a PITP that transfers PI and squalene without phosphatidylcholine transfer activity. To investigate the structural determinants for ligand specificity and transport in Sfh2, crystal structures of Sfh2 in complex with PI and squalene were determined at 1. Read More

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Kinetic and structural studies of the reaction of Escherichia coli dihydrodipicolinate synthase with (S)-2-bromopropionate.

Acta Crystallogr D Struct Biol 2022 Jul 8;78(Pt 7):846-852. Epub 2022 Jun 8.

Chemistry and Biochemistry Department, University of Oklahoma, 101 Stephenson Parkway, Norman, OK 73019, USA.

Dihydrodipicolinate synthase (DHDPS) catalyzes the first committed step in the lysine-biosynthetic pathway converting pyruvate and L-aspartate-β-semialdehyde to dihydrodipicolinate. Kinetic studies indicate that the pyruvate analog (S)-2-bromopropionate inactivates the enzyme in a pseudo-first-order process. An initial velocity pattern indicates that (S)-2-bromopropionate is a competitive inhibitor versus pyruvate, with an inhibition constant of about 8 mM. Read More

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