704,181 results match your criteria Acta Paulista de Enfermagem[Journal]

In-situ preparation of hollow CdCoS heterojunction with enhanced photocurrent response for highly photoelectrochemical sensing of organophosphorus pesticides.

Anal Chim Acta 2022 Jun 10;1212:339913. Epub 2022 May 10.

Department of Chemistry and Laboratory for Preparation and Application of Ordered Structural Materials of Guangdong Province, Shantou University, Shantou, Guangdong, 515063, PR China; Analysis & Testing Center, Shantou University, Shantou, Guangdong, 515063, PR China. Electronic address:

In this study, a porous hollow CdCoS(2) microsphere was synthesized based on the ZIF-67-S MOFs derived method of sulfurization reaction and calcination process. Under visible light irradiation, the resulting CdCoS(2) composite showed a markedly enhanced photoelectrochemical (PEC) response. The photocurrent value of the CdCoS(2) modified ITO electrode was 93-fold and 41-fold than that of CoS and CdS materials, respectively. Read More

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Increasing insulin measurement throughput by fluorescence anisotropy imaging immunoassays.

Anal Chim Acta 2022 Jun 14;1212:339942. Epub 2022 May 14.

Department of Chemistry and Biochemistry, Florida State University, 95 Chieftain Way, Tallahassee, FL, 32306, USA. Electronic address:

Insulin secreted from islets of Langerhans is the main hormone to reduce blood glucose. Examination of insulin secretion patterns at the single islet level reveals functional differences in the timings and patterns of release. This heterogeneous response highlights the importance of developing systems to measure dynamic release from small numbers of islets in parallel. Read More

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Sensitive detection of exosomes by gold nanoparticles labeling inductively coupled plasma mass spectrometry based on cholesterol recognition and rolling circle amplification.

Anal Chim Acta 2022 Jun 13;1212:339938. Epub 2022 May 13.

Department of Chemistry, Wuhan University, Wuhan, 430072, China. Electronic address:

Exosomes, potential biomarkers for liquid biopsy, provide a promising opportunity for early cancer diagnosis in a noninvasive manner. However, direct analysis of exosomes in complicated biological samples is still challenging. Herein, a cholesterol recognition assay was proposed for detection of exosomes by gold nanoparticles (Au NPs) labeling inductively coupled plasma mass spectrometry. Read More

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VIR-CRISPR: Visual in-one-tube ultrafast RT-PCR and CRISPR method for instant SARS-CoV-2 detection.

Anal Chim Acta 2022 Jun 13;1212:339937. Epub 2022 May 13.

Clinical Laboratory, Linping Campus, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 311100, China; School of Life Sciences, Fudan University, Shanghai, 200438, China. Electronic address:

Until now, corona virus disease 2019 (COVID-19) remained to be an enormous threat for global health. As one viral illness induced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), versatile, rapid and sensitive method for SARS-CoV-2 detection in early stage is urgently needed. Here, we reported an ultrasensitive and visual in-one-tube detection method which could be accomplished within half an hour from sampling-to-result. Read More

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Simultaneous measurements of Cr, Cd, Hg and Pb species in ng L levels by interfacing high performance liquid chromatography and inductively coupled plasma mass spectrometry.

Anal Chim Acta 2022 Jun 13;1212:339935. Epub 2022 May 13.

College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, 311121, China. Electronic address:

Toxicity, mobility, bioavailability and biofunctions of chromium, cadmium, mercury and lead are heavily dependent upon their specific chemical forms, leading to a high demand to metal speciation analysis rather than total quantification. Simultaneous speciation analysis of multiple metal(loid)s is attractive to a large sample capacity containing unstable analytes due to its economic and environmental advantages over the conventional single elemental strategies. In this work, an analytical method integrating online solid phase extraction into high performance liquid chromatography interfaced with inductively coupled plasma mass spectrometry (ICP-MS) to simultaneously preconcentrate and quantify Cr, Cd, Hg and Pb forms in pg L levels in water was developed. Read More

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A novel methyl-dependent DNA endonuclease GlaI coupling with double cascaded strand displacement amplification and CRISPR/Cas12a for ultra-sensitive detection of DNA methylation.

Anal Chim Acta 2022 Jun 8;1212:339914. Epub 2022 May 8.

Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China; National Facility for Translational Medicine, Shanghai Jiao Tong University, Shanghai, 200240, PR China. Electronic address:

Detection of methylation changes associated with oncogenic transformation is essential for early screening and treatment of cancer. Herein, we propose a novel DNA methylation detection assay based on the methyl-dependent DNA endonuclease GlaI coupling with double cascaded strand displacement amplification and CRISPR/Cas12a (GlaI-DC-SDA-CRISPR/Cas12a). The GlaI enables highly specific recognition and digestion of methylated target site (dsDNA) but leaves unmethylated target intact. Read More

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Wearable transdermal colorimetric microneedle patch for Uric acid monitoring based on peroxidase-like polypyrrole nanoparticles.

Anal Chim Acta 2022 Jun 7;1212:339911. Epub 2022 May 7.

Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, Shandong Key Laboratory of Biochemical Analysis, Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China. Electronic address:

Regular Uric Acid (UA) monitoring is essential for patients with gout, and great efforts have been made to develop portable, low-cost and easy-to-operate UA detection technologies. Herein, we developed a wearable microneedle colorimetric patch integrating the sampling function and the capability of real-time UA analysis in a minimally invasive manner, which can dramatically improve patient compliance. For the operation of the constructed device, the poly(vinyl alcohol) based microneedle was embedded with the uricase which catalyzed the oxidation of UA extracted from the interstitial fluids and produced HO. Read More

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In situ spatiotemporal solute imaging of metal corrosion on the example of magnesium.

Anal Chim Acta 2022 Jun 6;1212:339910. Epub 2022 May 6.

Department General, Analytical and Physical Chemistry, Chair of General and Analytical Chemistry, Montanuniversität Leoben, Franz-Josef-Strasse 18, 8700, Leoben, Austria. Electronic address:

Visualization and quantification of corrosion processes is essential in materials research. Here we present a new approach for 2D spatiotemporal imaging of metal corrosion dynamics in situ. The approach combines time-integrated Mg flux imaging by diffusive gradients in thin films laser ablation inductively coupled plasma mass spectrometry (DGT LA-ICP-MS) and near real-time pH imaging by planar optodes. Read More

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Detection of SARS-CoV-2 RNA through tandem isothermal gene amplification without reverse transcription.

Anal Chim Acta 2022 Jun 6;1212:339909. Epub 2022 May 6.

Department of Bioscience and Biotechnology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05902, Republic of Korea. Electronic address:

Diagnosis of SARS-CoV-2 infection through rapid, accurate, and sensitive testing is the most important and fundamental step in coping with the COVID-19 epidemic. We have developed a sensitive fluorometric assay to detect SARS-CoV-2 viral RNA without thermal cycling. This assay system, based on tandem isothermal gene amplification (TIGA), is composed of ternary rolling circle amplification (t-RCA) and subsequent strand displacement amplification (SDA) coupled with G-quadruplex-generating RCA (SDA/GQ-RCA). Read More

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Silver-fluoropolymer (Ag-CF) films: Kinetic study of silver release, and spectroscopic-microscopic insight into the inhibition of P. fluorescens biofilm formation.

Anal Chim Acta 2022 Jun 5;1212:339892. Epub 2022 May 5.

Institute of Analytical and Bioanalytical Chemistry, Ulm University, Albert Einstein Allee, 11, 89081, Ulm, Germany. Electronic address:

Silver-fluoropolymer (Ag-CF) composed of encapsulated bioactive nanophases within a thin polymer coating are promising antimicrobial films with excellent bioactivity. In this contribution, we report on Ag-CF thin films obtained by ion beam co-sputtering, accurately tuning film thickness, and inorganic loading. The Ag-CF films were characterized by spectroscopic and scanning probe microscopy techniques with respect to composition and swelling behavior. Read More

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New insights on the analysis of phytosterols in pollen and anther wall of tree peony (Paeonia ostii 'Fengdan') revealed by GC-MS/MS.

Anal Chim Acta 2022 Jun 2;1212:339891. Epub 2022 May 2.

Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China; University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address:

Phytosterols exhibit a wide range of pharmacological activities and have no side-effects, which have attracted more and more attention. In this study, two pre-treatment methods (alkaline hydrolysis and acid-alkali hydrolysis) were performed in parallel for the first time to investigate the effects on phytosterol analysis. The aim is to develop an analytical method for phytosterols in pollen and anther wall of tree peony (Paeonia ostii 'Fengdan') by gas chromatography-mass spectrometry. Read More

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Widely quasi-quantitative analysis enables temporal bile acids-targeted metabolomics in rat after oral administration of ursodeoxycholic acid.

Anal Chim Acta 2022 Jun 10;1212:339885. Epub 2022 May 10.

School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 100029, China. Electronic address:

Bile acid (BA) pool homeostasis can be disturbed when cholestasis, and ursodeoxycholic acid (UDCA) treatment is able to rectify the perturbed pool. Efforts were made here to propose a strategy enabling BAs-focused widely quantitative metabolomics and subsequently to lucubrate BA pool fluctuation trajectory in rats after dosing UDCA. A so-called universal metabolome standard (UMS) sample containing numerous natural BAs was constructed by involving various available BAs-enriched matrices. Read More

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Electrochemical biosensors based on nanomaterials for aflatoxins detection: A review (2015-2021).

Anal Chim Acta 2022 Jun 26;1212:339658. Epub 2022 Feb 26.

NanoBioAnalysis Group-Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería 8, 33006, Oviedo, Spain; Biotechnology Institute of Asturias, University of Oviedo, Santiago Gascon Building, 33006, Oviedo, Spain. Electronic address:

Due to their immunotoxicity, aflatoxins are considered as very important natural contaminants of a wide variety of agricultural products. Although conventional techniques such as high-performance liquid chromatography (HPLC) and gas chromatography (GC) combined with mass spectrometry (MS) have traditionally been used to detect such food contaminants, they are relatively expensive, laborious, and time consuming which limits their use for in field analysis at the so-called point-of-care (POC). Electrochemical biosensors are emerging devices that meet these expectations since they rely in reliable, simple, inexpensive, portable, selective, and easy-to-use analytical procedures and instruments that can be used by unskilled personnel outside the laboratory. Read More

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Hypergastrinemia, a clue leading to the identification of an atypical form of diabetes mellitus type 2.

Clin Chim Acta 2022 May 24. Epub 2022 May 24.

Department of Clinical Chemistry, Ghent University Hospital, Ghent, Belgium. Electronic address:

Background: A hitherto undescribed form of diabetes mellitus type 2 is reported in a Flemish family. In these patients, markedly elevated gastrin levels were observed, which could not be linked to gastrointestinal symptoms.

Materials And Methods: Gel permeation chromatography was performed for gastrin, insulin, and proinsulin. Read More

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Haemoproteus gabaldoni a valid species? An approach from morphology and molecular tools applied to parasites of Anseriformes.

Acta Trop 2022 May 24:106540. Epub 2022 May 24.

Departamento de Biología, Facultad de Ciencias, Universidad Nacional de Colombia, Sede Bogotá, Colombia. Electronic address:

Currently, there are three recognized species of haemoproteids infecting Anseriformes: Haemoproteus nettionis, H. macrovacuolatus, and H. greineri. Read More

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Therapeutic effect of heat-killed Lactobacillus plantarum L-137 on the gut health and growth of broilers.

Acta Trop 2022 May 24:106537. Epub 2022 May 24.

Department of Technology, Science and International Affair, HUTECH University, 475A Dien Bien Phu Street, Ward 25, Binh Thanh District, Ho Chi Minh City, 700000, Vietnam. Electronic address:

Although some studies on the effects of para-probiotics on the immune system and intestinal health have been conducted independently of research on antibiotics as growth promoters. This study investigated the effects of heat-killed Lactobacillus plantarum L-137 (L-137) and antibiotics as preventive and/or therapeutic substances for broilers against subclinical necrotic enteritis caused by Clostridium perfringens (CP). In total, 300 1-day-old broilers (46. Read More

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Ornithodoros cf. mimon infected with a spotted fever group Rickettsia in Brazil.

Acta Trop 2022 May 24:106541. Epub 2022 May 24.

Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zootechny, University of São Paulo, São Paulo, Brazil.

Ornithodoros mimon is an argasid tick primarily associated with bats that also infest other animals including birds, opossums and humans. In this paper, we report the finding of an argasid species resembling O. mimon, which similarly may be found in human dwellings and parasitize humans in Brazil. Read More

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Novel molecular approaches to combat vectors and vector-borne viruses: Special focus on RNA interference (RNAi) mechanisms.

Acta Trop 2022 May 24:106539. Epub 2022 May 24.

State Virology Laboratory, Department of Microbiology, Gandhi Medical College, Bhopal 462001, Madhya Pradesh, India.

Vector-borne diseases, such as dengue, chikungunya, zika, yellow fever etc pose significant burden among the infectious diseases globally, especially in tropical and sub-tropical regions. Globalization, deforestation, urbanization, climate change, uncontrolled population growth, inadequate waste management and poor vector-management infrastructure have all contributed to the expansion of vector habitats and subsequent increase in vector-borne diseases throughout the world. Conventional vector control methods, such as use of insecticides, have significant negative environmental repercussions in addition to developing resistance in vectors. Read More

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Factors associated with body image perception of adolescents.

Hyunju Chae

Acta Psychol (Amst) 2022 May 24;227:103620. Epub 2022 May 24.

Department of Nursing, Joongbu University, Geumsan, Republic of Korea. Electronic address:

This study aimed to confirm the status of body image perception by adolescents in South Korea and identify the associated factors using the data from the 14th Korea Youth Risk Behavior Survey (KYRBS) conducted in 2018. The KYRBS is a government-approved statistical survey performed annually and an anonymous self-administered online survey conducted in middle and high school students. The data for this study were downloaded from the KYRBS web site and analyzed through complex sample analysis. Read More

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U6/miR-211 expression ratio as a purity parameter for HEK293 cell-derived exosomes.

Acta Biochim Pol 2022 May 27. Epub 2022 May 27.

Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Łódź, Poland.

Small extracellular vesicles (sEVs) including exosomes are produced by all cell types and can be isolated from biological fluids and cell culture supernatants. The separation of exosomes with high purity from protein-rich media remains challenging. Besides contaminating proteins, small microvesicles (MVs) and apoptotic bodies are usually co-isolated with exosomes. Read More

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Use of dissected paraffin block tissue as a source of mRNA for transcriptional profiling and biomarker identification: a review, with preliminary findings in adrenocortical carcinoma tissue.

Acta Biochim Pol 2022 May 27. Epub 2022 May 27.

King's College Hospital, London, SE5 9RS UK.

Cancer tissue obtained by biopsy or at surgery is usually handled and stored as formalin-fixed, paraffin-embedded (FFPE) blocks. While primarily prepared for histological examination, this material represents a valuable resource that is stable and transportable, and which is ideal for examination in 3 dimensions. It has been used for DNA and protein analysis but the potential for transcriptional profiling has been little exploited: the quality of the extracted RNA is uncertain and there are few reports of its efficient use. Read More

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Self-perception of orofacial appearance: Brazil-Finland cross-national study.

Acta Odontol Scand 2022 May 27:1-9. Epub 2022 May 27.

Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland.

Objective: (i) To study the measurement invariance of Orofacial Esthetic Scale (OES) and Psychosocial Impact of Dental Aesthetics Questionnaire (PIDAQ), (ii) to compare the perception of orofacial appearance (OA) and (iii) to study the frequency of individuals who have sought or received aesthetic dental treatment between Brazil and Finland.

Methods: This was a cross-sectional observational study with snowball non-probability sample selection. Students and staff from universities in Finland and Brazil were invited to participate. Read More

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Efficacy of steroid treatment for sudden sensorineural hearing loss in patients with vestibular schwannoma.

Acta Otolaryngol 2022 May 27:1-4. Epub 2022 May 27.

Department of Otorhinolaryngology-Head and Neck Surgery, Soonchunhyang University Bucheon Hospital, Soonchunhyang University School of Medicine, Bucheon, Republic of Korea.

Background: Sudden sensorineural hearing loss (SSNHL) has several aetiologies and may be a presenting symptom of vestibular schwannoma (VS).

Objectives: The aim of this study was to investigate the recovery rate after steroid treatment and the prognostic factors of SSNHL with VS.

Materials And Methods: This was a retrospective observational study wherein 32 patients with VS who presented with SSNHL were analysed at a tertiary referral centre. Read More

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Engineering of 2D artificial nanozyme-based blocking effect-triggered colorimetric sensor for onsite visual assay of residual tetracycline in milk.

Mikrochim Acta 2022 May 27;189(6):233. Epub 2022 May 27.

School of Food & Biological Engineering, Key Laboratory for Agricultural Products Processing of Anhui Province, Hefei University of Technology, Hefei, 230009, China.

Accurate and low-cost onsite assay of residual antibiotics in food and agriculture-related matrixes (e.g., milk) is of significant importance for evaluating and controlling food pollution risk. Read More

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Decellularized Periosteum-Derived Hydrogels Promote the Proliferation, Migration and Osteogenic Differentiation of Human Umbilical Cord Mesenchymal Stem Cells.

Gels 2022 May 10;8(5). Epub 2022 May 10.

Department of Stomatology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Science, Guangzhou 510080, China.

Human umbilical cord mesenchymal stem cells (hUCMSCs) are promising for bone tissue engineering, which have a non-invasive harvesting process, high cell yield, favorable proliferation capacity, and low immunogenicity. However, the osteogenic efficacy of hUCMSCs is relatively lower than that of bone marrow mesenchymal stem cells (BMSCs). Hydrogels from decellularized extracellular matrix (dECM) preserve the biological compositions and functions of natural ECM, which can provide tissue-specific cues to regulate phenotypic expression and cell fate. Read More

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The effect of laryngopharyngeal reflux on arousal in patients with obstructive sleep apnea.

Acta Otolaryngol 2022 May 27:1-5. Epub 2022 May 27.

Department of Otolaryngology, General Hospital of Northern Theater Command, Shenyang, China.

Background: Laryngopharyngeal reflux (LPR) may be associated with the severity of obstructive sleep apnea (OSA). However, it is unknown whether LPR may be associated with arousal.

Objective: To clarify the effect of LPR on arousal in OSA. Read More

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[Open Pelvic Fractures with a Faringer I Zone Injury: a Set of 3 Case Reports Treated in 2020].

Acta Chir Orthop Traumatol Cech 2022 ;89(2):164-170

Katedra intenzivní medicíny, urgentní medicíny a forenzních oborů, Lékařská fakulta Ostravské univerzity, Ostrava.

Open pelvic fractures are devastating injuries with high mortality and morbidity that require aggressive treatment and multidisciplinary approach to achieve the best results. We present three cases of open pelvic fracture with a Faringer I zone injury, which were treated at our level I trauma centre in 2020. The patients were treated with external fixation without the need for fecal diversion. Read More

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January 2022

[Coccygodynia: Case Reports and Literature].

Acta Chir Orthop Traumatol Cech 2022 ;89(2):158-163

Neurochirurgická klinika Fakultní nemocnice Olomouc.

Coccygodynia, or tailbone pain, is the most common in women after trauma (complicated childbirth, fall). This pain can be treated conservatively (by using analgesics, local injections, physiotherapy) or by surgical coccygectomy. In the presented article, a set of five female patients is evaluated, in whom, after the failing conservative therapy, coccygectomy was indicated for persistent coccygodynia. Read More

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January 2022

Prophylactic Cerclage Cabling Decreases the Intraoperative Periprosthetic Fracture Rate during Cement-Less Total Hip Arthroplasty in Patients with Severe Developmental Hip Dysplasia.

Acta Chir Orthop Traumatol Cech 2022 ;89(2):150-157

Istanbul University-Cerrahpasa, Cerrahpasa Faculty of Medicine, Istanbul, Turkey.

PURPOSE OF THE STUDY Cementless stems in highly dysplastic hips are considered to increase the risk of femoral fracture and associated morbidity. Several authors speculated to use prophylactic cabling in this patient group to prevent intraoperative fractures. This study aims to reveal objective results regarding the perioperative complications in a large and consecutive patient group with respect to use of prophylactic cabling. Read More

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January 2022

Weight-Bearing Restrictions after Acetabular Fracture, Necessity or False Hope? A Brief Observational Study.

Acta Chir Orthop Traumatol Cech 2022 ;89(2):146-149

University Hospital Tuebingen; on behalf of the Eberhard-Karls-University Tuebingen, Faculty of Medicine; BG Hospital Tuebingen, Germany.

PURPOSE OF THE STUDY Most common postoperative treatment recommendations after acetabulum fractures suggest at least 6 weeks of postoperative partial or non weight-bearing. To protect the osteosynthetic construct this surgically set weight-bearing limit is trained by physical therapy. Aim of our analysis was to determine the free field patient compliance to these weight-bearing restrictions and observe their influence on the early postoperative radiographic imaging. Read More

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January 2022