519 results match your criteria Acta Naturae[Journal]


Relationship between the mRNA Expression Levels of Calpains 1/2 and Proteins Involved in Cytoskeleton Remodeling.

Acta Naturae 2020 Jan-Mar;12(1):110-113

Tomsk National Research Medical Center of the Russian Academy of Sciences, Tomsk, 634009 Russia.

Remodeling of the cytoskeleton underlies various cellular processes, including those associated with metastasis. The role of the proteases and proteins involved in cytoskeletal reorganization is being actively studied. However, there are no published data on the relationship between the mRNA expression levels of calpains 1/2 (CAPN 1/2) and the proteins associated with cytoskeleton remodeling. Read More

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http://dx.doi.org/10.32607/actanaturae.10947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245958PMC

Lipophilic Prodrug of Methotrexate in the Membrane of Liposomes Promotes Their Uptake by Human Blood Phagocytes.

Acta Naturae 2020 Jan-Mar;12(1):99-109

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997 Russia.

Previously, we showed that incorporation of methotrexate (MTX) in the form of a lipophilic prodrug (MTXDG) in 100-nm lipid bilayer liposomes of egg phosphatidylcholine can allow one to reduce toxicity and improve the antitumor efficiency of MTX in a mouse model of T-cell leukemic lymphoma. However, in our hemocompatibility tests , MTX liposomes caused complement (C) activation, obviously due to binding on the liposome surface and fragmentation of the C3 complement factor. In this work, we studied the interactions of MTX liposomes carrying stabilizing molecules phosphatidylinositol (PI), ganglioside GM1, or a lipid conjugate of -carboxymethylated oligoglycine (CMG) in the bilayer with subpopulations of human blood leukocytes. Read More

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http://dx.doi.org/10.32607/actanaturae.10946DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245962PMC

The Mechanisms of Cardiac Protection Using a Synthetic Agonist of Galanin Receptors during Chronic Administration of Doxorubicin.

Acta Naturae 2020 Jan-Mar;12(1):89-98

National Medical Research Center for Cardiology, Moscow, 121552 Russia.

The use of the anticancer drug doxorubicin (Dox) is limited by its cardiotoxic effect. The aim of this work was to study the effect of a new synthetic agonist of the galanin receptor GalR1-3 [βAla14, His15]-galanine (2-15) (G) on the metabolism, antioxidant enzyme activity, and cardiac function in rats with cardiomyopathy (CM) caused by chronic administration of Dox. Coadministration of peptide G and Dox significantly increased the fractional shortening (FS) and ejection fraction (EF) by an average of 30 ± 4% compared with the indices in the Dox group. Read More

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http://dx.doi.org/10.32607/actanaturae.10945DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245963PMC

The Telomeric Cdc13 Protein from Yeast Hansenula polymorpha.

Acta Naturae 2020 Jan-Mar;12(1):84-88

Faculty of Chemistry and Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991 Russia.

Telomeres are special structures at the ends of chromosomes that play an important role in the protection of the genetic material. Telomere composition is very diverse; noticeable differences can often be observed even among closely related species. Here, we identify the homolog of telomeric protein Cdc13 in the thermotolerant yeast . Read More

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http://dx.doi.org/10.32607/actanaturae.10944DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245964PMC

Subtypes of Neurohypophyseal Nonapeptide Receptors and Their Functions in Rat Kidneys.

Acta Naturae 2020 Jan-Mar;12(1):73-83

Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences, Saint Petersburg, 194223 Russia.

The nonapeptides of neurohypophysis, vasotocin and mesotocin, detected in most vertebrates, are replaced by vasopressin and oxytocin in mammals. Using bioinformatics methods, we determined the spectrum of receptor subtypes for these hormones in mammals and their physiological effects in the kidneys of rats. A search for sequences similar to the vertebrate vasotocin receptor by proteomes and transcriptomas of nine mammalian species and the rat genome revealed three subtypes of vasopressin receptors (V1a, V1b, and V2) and one type of oxytocin receptors. Read More

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http://dx.doi.org/10.32607/actanaturae.10943DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245957PMC

The Behavioral and Neurochemical Aspects of the Interaction between Antidepressants and Unpredictable Chronic Mild Stress.

Acta Naturae 2020 Jan-Mar;12(1):63-72

Sechenov First Moscow State Medical University (Sechenov University), Moscow, 119991 Russia.

The behavioral and neurochemical effects of amitriptyline (10 mg/kg, i.p.) and fluoxetine (20 mg/kg, i. Read More

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http://dx.doi.org/10.32607/actanaturae.10942DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245955PMC

The Genome Structure of Ciprofloxacin-Resistant Mycoplasma Hominis Clinical Isolates.

Acta Naturae 2020 Jan-Mar;12(1):56-62

Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology, Federal Service for Surveillance on Customers Rights Protection and Human Wellbeing, Nizhniy Novgorod, 603950 Russia.

The genome structure of three ciprofloxacin-resistant clinical isolates was studied using next-generation sequencing on the Illumina platform. The protein sequences of the studied strains were found to have a high degree of homology. (M45, M57, MH1866) was shown to have limited biosynthetic capabilities, associated with the predominance of the genes encoding the proteins involved in catabolic processes. Read More

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http://dx.doi.org/10.32607/actanaturae.10941DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245959PMC

Establishment of a FDC-P1 Murine Cell Line with Human KIT N822K Gene Overexpression.

Acta Naturae 2020 Jan-Mar;12(1):51-55

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.

The mechanism of resistance of leukemia cells to chemotherapeutic drugs remains poorly understood. New model systems for studying the processes of malignant transformation of hematopoietic cells are needed. Based on cytokine-dependent murine acute myeloid leukemia (AML) FDC-P1 cells, we generated a new cell line with ectopic expression of the gene encoding mutant human receptor tyrosine kinase (N822K). Read More

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http://dx.doi.org/10.32607/actanaturae.10938DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245965PMC

Simple Recommendations for Improving Efficiency in Generating Genome-Edited Mice.

Acta Naturae 2020 Jan-Mar;12(1):42-50

Institute of functional genomics, Lomonosov Moscow State University, Moscow, 119991 Russia.

The generation of transgenic model organisms (primarily mice) is an integral part of modern fundamental and applied research. Simple techniques based on the biology of these laboratory rodents can often increase efficiency when generating genome-edited mouse strains. In this study, we share our three years of experience in the optimization of mouse genome editing based on microinjection of CRISPR/Cas9 components into ca. Read More

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http://dx.doi.org/10.32607/actanaturae.10937DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245961PMC

Genes that Control Vaccinia Virus Immunogenicity.

Acta Naturae 2020 Jan-Mar;12(1):33-41

State Research Center of Virology and Biotechnology "Vector", Rospotrebnadzor, Novosibirsk region, Koltsovo, 630559 Russia.

The live smallpox vaccine was a historical first and highly effective vaccine. However, along with high immunogenicity, the vaccinia virus (VACV) caused serious side effects in vaccinees, sometimes with lethal outcomes. Therefore, after global eradication of smallpox, VACV vaccination was stopped. Read More

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http://dx.doi.org/10.32607/actanaturae.10935DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245956PMC

Stochastics of Degradation: The Autophagic-Lysosomal System of the Cell.

Acta Naturae 2020 Jan-Mar;12(1):18-32

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Moscow, 117997 Russia.

Autophagy is a conservative and evolutionarily ancient process that enables the transfer of various cellular compounds, organelles, and potentially dangerous cellular components to the lysosome for their degradation. This process is crucial for the recycling of energy and substrates, which are required for cellular biosynthesis. Autophagy not only plays a major role in the survival of cells under stress conditions, but is also actively involved in maintaining cellular homeostasis. Read More

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http://dx.doi.org/10.32607/actanaturae.10936DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245954PMC

Spinal Cord Microglia in Health and Disease.

Acta Naturae 2020 Jan-Mar;12(1):4-17

Institute of Experimental Medicine, St. Petersburg, 197376 Russia.

The review summarizes data of recent experimental studies on spinal microglia, the least explored cells of the spinal cord. It focuses on the origin and function of microglia in mammalian spinal cord embryogenesis. The main approaches to the classification of microgliocytes based on their structure, function, and immunophenotypic characteristics are analyzed. Read More

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http://dx.doi.org/10.32607/actanaturae.10934DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245960PMC

Tear Fluid Catecholamines As Biomarkers of the Parkinson's Disease: A Clinical and Experimental Study.

Acta Naturae 2019 Oct-Dec;11(4):99-103

Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Moscow, 119334 Russia.

An important approach to an early diagnosis of Parkinson's disease (PD) is screening for peripheral biomarkers in patients at the early clinical stage. In this study, we evaluated catecholamine concentration changes in the tear fluid of untreated PD patients as biomarkers. Norepinephrine and dopamine concentrations in the tear fluid of patients were found to increase compared to those in age controls, which was especially pronounced on the side where motor symptoms appeared. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-4-99-103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977954PMC
January 2020

Genetic Variability of the AcrAB-TolC Multidrug Efflux Pump Underlies SkQ1 Resistance in Gram-Negative Bacteria.

Acta Naturae 2019 Oct-Dec;11(4):93-98

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991 Russia.

SkQ1, a novel antibiotic targeting bacterial bioenergetics, is highly effective against both gram-positive and gram-negative bacteria. However, some gram-negative bacteria, such as and , are highly resistant to it. In different gram-negative bacteria, this resistance is associated with the identity of their AcrB transporter protein sequence with the sequence of the AcrB protein from . Read More

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http://dx.doi.org/10.32607/20758251-2019-11-4-93-98DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977953PMC
January 2020

Calcitonin Gene-Related Peptide and Substance P As Predictors of Venous Pelvic Pain.

Acta Naturae 2019 Oct-Dec;11(4):88-92

Pirogov Russian National Research Medical University, Moscow, 119049 Russia.

The purpose of this work was to study the contents of calcitonin gene-related peptide (CGRP) and substance P (SP) in the blood plasma of patients with pelvic varicose veins. Thirty women with pelvic varicosities and a reflux blood flow were investigated using duplex ultrasonography. Group 1 included 18 patients with clinical signs of the pelvic congestion syndrome (PCS), including venous pelvic pain (VPP). Read More

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http://dx.doi.org/10.32607/20758251-2019-11-4-88-92DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977958PMC
January 2020

"Shielding" of Cytokine Induction by the Periodontal Microbiome in Patients with Periodontitis Associated with Type 2 Diabetes Mellitus.

Acta Naturae 2019 Oct-Dec;11(4):79-87

A.I. Evdokimov Moscow State University of Medicine and Dentistry of the Ministry of Healthcare of the Russian Federation, Moscow, 127473 Russia.

Periodontal diseases, especially those with polymicrobial etiology, are often associated with type 2 diabetes mellitus, proceeding more severely and affecting the course of diabetes mellitus. Recently, this feature has been associated with the ability of periodontopathogen microflora to cause not only a local infectious process in the oral cavity, but also to interact with the human immune system and induce various systemic effects. We investigated changes in the salivary cytokine profile of patients with chronic periodontitis, associated and not associated with type 2 diabetes mellitus. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-4-79-87DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977959PMC
January 2020

Surface Charge Mapping on Virions and Virus-Like Particles of Helical Plant Viruses.

Acta Naturae 2019 Oct-Dec;11(4):73-78

Department of Virology, Lomonosov Moscow State University, Moscow, 119234 Russia.

Currently, the assembly of helical plant viruses is poorly understood. The viral assembly and infection may be affected by the charge distribution on the virion surface. However, only the total virion charge (isoelectric point) has been determined for most plant viruses. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-4-73-78DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977955PMC
January 2020

The Role of Heritable Tumors in Evolution of Development: a New Theory of Carcino-evo-devo.

Authors:
A P Kozlov

Acta Naturae 2019 Oct-Dec;11(4):65-72

Vavilov Institute of General Genetics RAS, Moscow, 119333 Russia Biomedical Center, Research Institute of Ultrapure Biologicals and Peter the Great St. Petersburg Polytechnic University, St. Petersburg, 195251 Russia.

The hypothesis of evolution by tumor neofunctionalization (the "main hypothesis") describes the possible role of hereditary tumors in evolution. The present article examines the relationship of the main hypothesis to other biological theories. As shown in this paper, the main hypothesis does not contradict to the existing biological theories, but fills the lacunas between them and explains some unexplained (or not completely understood) questions. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-4-65-72DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977963PMC
January 2020

The Role of Interleukin-37 in the Pathogenesis of Allergic Diseases.

Acta Naturae 2019 Oct-Dec;11(4):54-64

National Research Center - Institute of Immunology Federal Medical-Biological Agency of Russia, Moscow, 115522 Russia.

Cytokines of the interleukin-1 (IL-1) family play an important role in the realization of the protective functions of innate immunity and are the key mediators involved in the pathogenesis of a wide range of diseases, including various manifestations of allergy. The IL-1 family includes more than 11 members. However, the functions of many of them remain to be elucidated. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-4-54-64DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977961PMC
January 2020

DARPins: Promising Scaffolds for Theranostics.

Acta Naturae 2019 Oct-Dec;11(4):42-53

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.

Monoclonal antibodies are the classical basis for targeted therapy, but the development of alternative binding proteins has made it possible to use non-immunoglobulin proteins as targeting modules. The advantages of DARPins, scaffold proteins based on ankyrin repeats, over antibodies are as follows: small size, stability over a wide range of temperatures and pH values, low aggregation tendency, and ease of production in heterologous expression systems. The differences in the structure of the paratope of DARPin and antibodies broaden the spectrum of target molecules, while the ease of creating hybrid fusion proteins allows one to obtain bispecific and multivalent constructs. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-4-42-53DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977956PMC
January 2020

The Role of Tumor-Derived Vesicles in the Regulation of Antitumor Immunity.

Acta Naturae 2019 Oct-Dec;11(4):33-41

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, 117997 Russia.

In this article, we present a comprehensive, updated, and elucidative review of the current knowledge on the function played by tumor-derived vesicles (TDVs) in the crosstalk between tumor and immune cells. Characterization of the structure, biogenesis, and the major functions of TDVs is reported. The review focuses on particular ways of suppression or activation of CD4/CD8 T cells by tumor-derived vesicles. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-4-33-41DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977957PMC
January 2020

Non-neutralizing Antibodies Directed at Conservative Influenza Antigens.

Acta Naturae 2019 Oct-Dec;11(4):22-32

Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya of the Ministry of Health of the Russian Federation, Moscow, 123098 Russia.

At the moment, developing new broad-spectrum influenza vaccines which would help avoid annual changes in a vaccine's strain set is urgency. In addition, developing new vaccines based on highly conserved influenza virus proteins could allow us to better prepare for potential pandemics and significantly reduce the damage they cause. Evaluation of the humoral response to vaccine administration is a key aspect of the characterization of the effectiveness of influenza vaccines. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-4-22-32DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977952PMC
January 2020

Double-Stranded RNAs in Plant Protection Against Pathogenic Organisms and Viruses in Agriculture.

Acta Naturae 2019 Oct-Dec;11(4):13-21

International Laboratory «Resistom», The Skolkovo Innovation Center, Moscow, 143026 Russia.

Recent studies have shown that plants are able to express the artificial genes responsible for the synthesis of double-stranded RNAs (dsRNAs) and hairpin double-stranded RNAs (hpRNAs), as well as uptake and process exogenous dsRNAs and hpRNAs to suppress the gene expression of plant pathogenic viruses, fungi, or insects. Both endogenous and exogenous dsRNAs are processed into small interfering RNAs (siRNAs) that can spread locally and systemically through the plant, enter pathogenic microorganisms, and induce RNA interference-mediated pathogen resistance in plants. There are numerous examples of the development of new biotechnological approaches to plant protection using transgenic plants and exogenous dsRNAs. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-4-13-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977960PMC
January 2020

The Contribution of Major Histocompatibility Complex Class II Genes to an Association with Autoimmune Diseases.

Acta Naturae 2019 Oct-Dec;11(4):4-12

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997 Russia.

Genetic studies of patients with autoimmune diseases have shown that one of the most important roles in the developing of these diseases is played by a cluster of genes of the major histocompatibility complex (MHC), as compared with other genome areas. Information on the specific contribution of MHC alleles, mostly MHC class II ones, to the genetic predisposition to autoimmune diseases is crucial for understanding their pathogenesis. This review dwells on the most relevant aspects of this problem: namely, the correlation between carriage of certain MHC II alleles and an increased (positively associated allele) or reduced (negatively associated allele) probability of developing the most common autoimmune diseases, such as type 1 diabetes, rheumatoid arthritis, multiple sclerosis, systemic lupus erythematosus, autoimmune thyroiditis, etc. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-4-4-12DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977962PMC
January 2020

A Unique Prototypic Device for Radiation Therapy: The p53-Independent Antiproliferative Effect of Neutron Radiation.

Acta Naturae 2019 Jul-Sep;11(3):99-102

Blokhin National Medical Center of Oncology, Kashirskoye Sh. 24, Moscow, 115478, Russia.

Radiation therapy with heavy particles including neutrons, an otherwise therapeutically perspective because of its high tissue penetration and efficient tumor damage, is currently limited by the lack of adequate equipment. An NG-24 generator (140 kg, 42 × 110 cm, ~1011 particles/s, > 14 MeV) has been designed and engineered to replace the huge and environmentally harmful neutron reactors, cyclotrons, and accelerators with a compact, portable, safe, and potent source of high-energy neutrons. We demonstrate that the neutron beam produced by NG-24 causes a significant antiproliferative effect on human tumor cell lines regardless of the status of the anti-apoptotic p53 protein. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-3-99-102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826147PMC
November 2019

An Abnormally High Closing Potential of the OMPF Porin Channel from Yersinia Ruckeri: The Role of Charged Residues and Intramolecular Bonds.

Acta Naturae 2019 Jul-Sep;11(3):89-98

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Leninskie Gory 1/40, Moscow, 119991, Russia.

Electrophysiological experiments on bilayer lipid membranes showed that the isolated outer membrane major porin of Yersinia ruckeri (YrOmpF) exhibits activity typical of porins from Gram-negative bacteria, forming channels with a mean conductance of 230 pS (in 0.1 M KCl) and slight asymmetry with respect to the applied voltage. Under acidic conditions (up to pH = 3. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-3-89-98DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826154PMC
November 2019

The Catalytic Mechanisms of the Reactions between Tryptophan Indole-Lyase and Nonstandard Substrates: The Role of the Ionic State of the Catalytic Group Accepting the Cα Proton of the Substrate.

Acta Naturae 2019 Jul-Sep;11(3):82-88

Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Vavilova Str. 28 , Moscow, 119991, Russia.

In the reaction between tryptophan indole-lyase (TIL) and a substrate containing a bad leaving group (L-serine), general acid catalysis is required for the group's elimination. During this stage, the proton originally bound to the C atom of the substrate is transferred to the leaving group, which is eliminated as a water molecule. As a result, the basic group that had accepted the C proton at the previous stage has to be involved in the catalytic stage following the elimination in its basic form. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-3-82-88DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826157PMC
November 2019

Surgical Simulation of a Posttraumatic Spinal Cord Glial Scar in Rats.

Acta Naturae 2019 Jul-Sep;11(3):75-81

Shemyakin-Ovchinnikov Institute of bioorganic chemistry, GSP-7, Miklukho-Maklaya Str. 16/10, Moscow, 117997, Russia.

We developed and verified an original, minimally invasive method for surgical simulation of a posttraumatic spinal cord glial scar in rats. The model is intended for use as a biological platform for testing the stimulation of regenerative processes in the central nervous system. Unification of the model enables one to achieve versatility both for implantation techniques and for the development of system-action approaches. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-3-75-81DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826152PMC
November 2019

Long Noncoding RNA LL35/Falcor Regulates Expression of Transcription Factor Foxa2 in Hepatocytes in Normal and Fibrotic Mouse Liver.

Acta Naturae 2019 Jul-Sep;11(3):66-74

Skolkovo Institute of Science and Technology, Bolshoy Blvd. 30, bldg. 1, Moscow, 121205, Russia.

Long noncoding RNAs (lncRNA) play important roles in the regulation of transcription, splicing, translation, and other processes in the cell. Human and mouse lncRNA (DEANR1 and LL35/Falcor, respectively) located in the genomic environment in close proximity to the Foxa2 transcription factor were discovered earlier. In this work, tissue-specific expression of LL35/Falcor lncRNA has been shown in mouse liver and lungs. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-3-66-74DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826158PMC
November 2019

The Structural and Immunological Properties of Chimeric Proteins Containing HIV-1 MPER Sites.

Acta Naturae 2019 Jul-Sep;11(3):56-65

State Research Center of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk region, 630559 , Russia.

The human immunodeficiency virus (HIV-1) poses a serious risk to global public health. The development of a safe and effective vaccine could stop the HIV/AIDS pandemic. Much of the research focused on HIV-1 prevention through vaccination is aimed at developing immunogens and immunization strategies to induce the formation of antibodies with neutralizing activity against a broad range of HIV-1 isolates (bNAbs). Read More

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http://dx.doi.org/10.32607/20758251-2019-11-3-56-65DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826149PMC
November 2019

The Effect of a Lipopolysaccharide from Rhodobacter capsulatus PG on Inflammation Caused by Various Influenza Strains.

Acta Naturae 2019 Jul-Sep;11(3):46-55

Institute of Basic Biological Problems of RAS, FRC PSCBR RAS, Science Ave. 3, Pushchino, Moscow, 142290, Russia.

The development of a specific inflammation in mice that had been infected by two influenza virus strains, A/chicken/Kurgan/5/2005 (H5N1) and A/Hamburg/2009 MA (H1N1), was studied. We investigated the effect of a non-toxic lipopolysaccharide from Rhodobacter capsulatus PG on the survival and body weight of the mice, production of IgG antibodies, and the induction of pro- and anti-inflammatory cytokines in blood serum. The administration of the R. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-3-46-55DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826150PMC
November 2019

The Mechanisms of Action of Triindolylmethane Derivatives on Lipid Membranes.

Acta Naturae 2019 Jul-Sep;11(3):38-45

Institute of Cytology, Russian Academy of Sciences, Tikhoretsky Ave. 4, St. Petersburg, 194064, Russia.

The effects of new synthetic antibacterial agents - tris(1-pentyl-1H-indol-3-yl)methylium chloride (LCTA-1975) and (1-(4-(dimethylamino)-2,5-dioxo-2,5-dihydro-1H-pyrrol-3-yl)-1H-indol-3-yl)bis(1-propyl- 1H-indol-3-yl)methylium chloride (LCTA-2701 - on model lipid membranes were studied. The ability of the tested agents to form ion-conductive transmembrane pores, influence the electrical stability of lipid bilayers and the phase transition of membrane lipids, and cause the deformation and fusion of lipid vesicles was investigated. It was established that both compounds exert a strong detergent effect on model membranes. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-3-38-45DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826156PMC
November 2019

A Nerve Growth Factor Dipeptide Mimetic Stimulates Neurogenesis and Synaptogenesis in the Hippocampus and Striatum of Adult Rats with Focal Cerebral Ischemia.

Acta Naturae 2019 Jul-Sep;11(3):31-37

Federal State Budgetary Institution "Zakusov Research Institute of Pharmacology", Baltiyskay Str. 8 , Moscow, 125315, Russia.

The nerve growth factor (NGF) and its mimetics, which have neuroprotective and neuroregenerative properties, are attractive candidates for developing new drugs for brain injury therapy. A dipeptide mimetic of NGF loop 4, bis(N-succinyl-L-glutamyl-L-lysine) hexamethylenediamide (GK-2), developed at the Zakusov Research Institute of Pharmacology, has the NGF-like ability to activate TrkA receptors, but unlike NGF, GK-2 activates mainly the PI3K/AKT pathway associated with neuroprotection and has no effect on the MAPK cascade associated with hyperalgesia, the main side effect of NGF. That GK-2 possesses neuroprotective activity has been observed in various models of cerebral ischemia. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-3-31-37DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826148PMC
November 2019

A Novel Sulfonated Derivative of β-Cyclodextrin Effectively Inhibits Influenza A Virus Infection in vitro and in vivo.

Acta Naturae 2019 Jul-Sep;11(3):20-30

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Lavrentiev Ave. 8, Novosibirsk, 630090, Russia.

The development of novel drugs against the influenza virus with high efficiency and low toxicity is an urgent and important task. Previous reports have demonstrated that compounds based on sulfo derivatives of oligo- and polysaccharides possess high antiviral activity. In this study, we have examined the ability of a novel sulfonated derivative of β-cyclodextrin (KS-6469) to inhibit the influenza virus A/WSN/33 (H1N1) infection in vitro and in vivo. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-3-20-30DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826151PMC
November 2019

Cold Physical Plasma Decreases the Viability of Lung Adenocarcinoma Cells.

Acta Naturae 2019 Jul-Sep;11(3):16-19

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Akad. Lavrentiev Ave. 8, Novosibirsk, 630090, Russia.

The high mortality rate that accompanies cancer spurs the search for new methods that can be used to treat malignant neoplasms. In addition to chemotherapy, electrophysical techniques for tumor treatment appear rather promising. The results of in vitro exposure of A549 human lung adenocarcinoma cells to cold atmospheric plasma (CAP) are hereby presented. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-3-16-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826155PMC
November 2019

The Molecular Mechanisms of Gametic Incompatibility in Invertebrates.

Acta Naturae 2019 Jul-Sep;11(3):4-15

Department of Invertebrate Zoology, Faculty of Biology, St Petersburg State University, Universitetskaya Emb. 7/9, St. Petersburg, 199034, Russia.

Fertilization (gamete fusion followed by zygote formation) is a multistage process. Each stage is mediated by ligand-receptor recognition of gamete interaction molecules. This recognition includes the movement of sperm in the gradient of egg chemoattractants, destruction of the egg envelope by acrosomal proteins, etc. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-3-4-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826153PMC
November 2019

Bacteriophage MS2 As a Tool for Targeted Delivery in Solid Tumor Chemotherapy.

Acta Naturae 2019 Apr-Jun;11(2):98-101

Biotechnologiya, Ltd., Efremova Str. 20, Moscow, 119048, Russia.

Bacteriophage MS2 was employed for targeted delivery of an apoptosis-inducing agent, Tl+, into a tumor tissue. The targeted delivery was ensured by iRGD peptide, a ligand of integrins presumably located on the surface of endotheliocytes of the tumor tissue neovasculature and certain tumor cells. The synthesized peptide was conjugated to MS2 capsid proteins. Read More

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http://www.actanaturae.ru/attachment.aspx?id=4265
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http://dx.doi.org/10.32607/20758251-2019-11-2-98-101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6643345PMC
August 2019
12 Reads

A New MicroRNA Cluster Involved in the Reprogramming to a Pluripotent State.

Acta Naturae 2019 Apr-Jun;11(2):92-97

The Federal Research Center Institute of Cytology and Genetics SB RAS, Lavrentyeva Ave. 10, Novosibirsk, 630090, Russia.

Reprogramming of somatic cells to a pluripotent state is a complex, multistage process that is regulated by many factors. Among these factors, non-coding RNAs and microRNAs (miRNAs) have been intensively studied in recent years. MiRNAs play an important role in many processes, particularly in cell reprogramming. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-2-92-97DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6643346PMC

The Profile of Post-translational Modifications of Histone H1 in Chromatin of Mouse Embryonic Stem Cells.

Acta Naturae 2019 Apr-Jun;11(2):82-91

Institute of Cytology of the Russian Academy of Sciences, Laboratory of Molecular Biology of Stem Cells, Tikhoretsky Ave. 4, St. Petersburg, 194064, Russia.

Linker histone H1 is one of the main chromatin proteins which plays an important role in organizing eukaryotic DNA into a compact structure. There is data indicating that cell type-specific post-translational modifications of H1 modulate chromatin activity. Here, we compared histone H1 variants from NIH/3T3, mouse embryonic fibroblasts (MEFs), and mouse embryonic stem (ES) cells using matrix-assisted laser desorption/ ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FT-ICR-MS). Read More

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http://dx.doi.org/10.32607/20758251-2019-11-2-82-91DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6643340PMC

Studying the Possibilities of Using 2-Halogen-Substituted Acetamides As Acyl Donors in Penicillin Acylase-Catalyzed Reactions.

Acta Naturae 2019 Apr-Jun;11(2):77-81

Lomonosov Moscow State University, Belozersky Institute of Physicochemical Biology, Lenin Hills 1 , bldg. 40, Moscow, 119991, Russia.

The possibility of using amides of halogen-substituted acetic acids as acyl donors in penicillin acylase-catalyzed reactions has been investigated, and the ability of this group of compounds to inactivate enzymes in the course of the catalytic conversion has been established. The strongest inactivating effect was demonstrated by iodoacetamide and bromoacetamide. However, the negative contribution of this side activity can be minimized by decreasing the temperature, when the rate of acyl donor conversion by penicillin acylases is still high enough, but the impact of enzyme inactivation becomes less significant. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-2-77-81DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6643344PMC

The Differential Anti-HIV Effect of a New Humic Substance-Derived Preparation in Diverse Cells of the Immune System.

Acta Naturae 2019 Apr-Jun;11(2):68-76

Gamaleya Center for Epidemiology and Microbiology. Gamaleya Str.1 8, Moscow, 123098, Russia.

The anti-HIV activity of a new humic substance-derived preparation has been studied in individual pools of immune cells (CD4+ T lymphocytes, macrophages, dendritic cells). Near-complete inhibition of the HIV infection (by more than 90%) was achieved by treating each of the abovementioned cell types with non-toxic concentrations of the preparation. The inhibitory effect demonstrates the possibility of preventing the depletion of a significant portion of functionally important immune cells. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-2-68-76DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6643347PMC
August 2019
1 Read

The Role of Recombinant Human Cyclophilin A in the Antitumor Immune Response.

Acta Naturae 2019 Apr-Jun;11(2):63-67

Federal State Budgetary Institution «N.N. Blokhin National Medical Research Center of Oncology» of the Ministry of Health of the Russian Federation, Kashirskoye Sh. 24, Moscow, 115478, Russia.

Cyclophilin A (CypA) is a multifunctional protein that exhibits an isomerase activity and exists in the intracellular and secretory forms. Secretory CypA promotes regeneration of the hematopoietic and the immune systems of an organism by stimulating stem cell migration from the bone marrow. New approaches based on CypA are currently being developed for the treatment of limb ischemia, neutralization of the side effects of Cyclosporine A (CsA) therapy, etc. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-2-63-67DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6643350PMC

Septin Polymerization Slows Synaptic Vesicle Recycling in Motor Nerve Endings.

Acta Naturae 2019 Apr-Jun;11(2):54-62

Kazan State Medical University, Butlerova Str. 49, Kazan, 420012, Russia.

Septins are GTP-binding proteins recognized as a component of the cytoskeleton. Despite the fact that septins are highly expressed by neurons and can interact with the proteins that participate in synaptic vesicle exocytosis and endocytosis, the role of septins in synaptic transmission and the synaptic vesicle recycling mechanisms is poorly understood. In this study, neurotransmitter release and synaptic vesicle exocytosis and endocytosis were investigated by microelectrode intracellular recording of end-plate potentials and fluorescent confocal microscopy in mouse diaphragm motor nerve endings during septin polymerization induced by forchlorfenuron application. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-2-54-62DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6643342PMC

"Green" Synthesis of Cytotoxic Silver Nanoparticles Based on Secondary Metabolites of Lavandula Angustifolia Mill.

Acta Naturae 2019 Apr-Jun;11(2):47-53

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, GSP-7, Miklukho-Maklaya Str. 16/10, Moscow, 117997, Russia.

In this study, we used "green" synthesis to prepare silver nanoparticles (NPs) from aqueous plant and callus extracts of the narrow-leaved lavender Mill. 35.4 ± 1. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-2-47-53DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6643349PMC

Assessment of the Phenylketonuria (PKU)-Associated Mutation p.R155H Biochemical Manifestations by Mass Spectrometry-Based Blood Metabolite Profiling.

Acta Naturae 2019 Apr-Jun;11(2):42-46

Joint Center for genomic, proteomic and metabolomics studies, Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev Ave. 8, Novosibirsk, 630090, Russia.

Homozygous siblings with different treatment histories represent an excellent model to study both the phenotypic manifestation of mutations and the efficacy of therapy. We compared phenylketonuria (PKU) manifestations in two different gender siblings who were homozygous carriers of a rare phenylalanine hydroxylase () mutation, p.R155H, subjected to different treatments. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-2-42-46DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6643343PMC
August 2019
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Targeted Drug Delivery in Lipid-like Nanocages and Extracellular Vesicles.

Acta Naturae 2019 Apr-Jun;11(2):28-41

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16 /10, Moscow, 117997, Russia.

The possibility of targeted drug delivery to a specific tissue, organ, or cell has opened new promising avenues in treatment development. The technology of targeted delivery aims to create multifunctional carriers that are capable of long circulation in the patient's organism and possess low toxicity at the same time. The surface of modern synthetic carriers has high structural similarity to the cell membrane, which, when combined with additional modifications, also promotes the transfer of biological properties in order to penetrate physiological barriers effectively. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-2-28-41DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6643341PMC

Molecular Biomarkers of Brain and Spinal Cord Astrocytomas.

Acta Naturae 2019 Apr-Jun;11(2):17-27

National Medical Research Center of Neurosurgery, Ministry of Health of the Russian Federation Acad. N.N. Burdenko, 4th Tverskaya-Yamskaya Str. 16, Moscow, 125047, Russia.

Spinal cord astrocytomas are rare diseases of the central nervous system. The localization of these tumors and their infiltrative growth complicate their surgical resection, increase the risk of postoperative complications, and require more careful use of radio- and chemotherapy. The information on the genetic mutations associated with the onset and development of astrocytomas provides a more accurate neoplasm diagnosis and classification. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-2-17-27DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6643348PMC
August 2019
1 Read

Application of Polyhydroxyalkanoates in Medicine and the Biological Activity of Natural Poly(3-Hydroxybutyrate).

Acta Naturae 2019 Apr-Jun;11(2):4-16

Faculty of Biology, M.V. Lomonosov Moscow State University, Leninskie Gory 1, bldg. 12, Moscow, 119234, Russia.

Biodegradable and biocompatible polymers, polyhydroxyalkanoates (PHAs), are actively used in medicine to produce a wide range of medical devices and dosage formulations. The medical industry mainly utilizes PHAs obtained by chemical synthesis, but interest in the medical application of natural PHAs obtained biotechnologically is also growing. Synthetic PHAs are the biomimetic analogs of bacterial poly(3-hydroxybutyrate) (PHB) and other natural PHAs. Read More

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http://dx.doi.org/10.32607/20758251-2019-11-2-4-16DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6643351PMC

Mauritian Endemic Medicinal Plant Extracts Induce G2/M Phase Cell Cycle Arrest and Growth Inhibition of Oesophageal Squamous Cell Carcinoma in Vitro.

Acta Naturae 2019 Jan-Mar;11(1):81-90

Department of Health Sciences, Faculty of Science, University of Mauritius, Réduit, 80837, Republic of Mauritius.

Terrestrial plants have contributed massively to the development of modern oncologic drugs. Despite the wide acceptance of Mauritian endemic flowering plants in traditional medicine, scientific evidence of their chemotherapeutic potential is lacking. This study aimed to evaluate the tumor cytotoxicity of leaf extracts from five Mauritian endemic medicinal plants, namely Willd (Euphorbiaceae), Bojer (Sapotaceae), Cav. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6475868PMC
April 2019
17 Reads

CASBench: A Benchmarking Set of Proteins with Annotated Catalytic and Allosteric Sites in Their Structures.

Acta Naturae 2019 Jan-Mar;11(1):74-80

Lomonosov Moscow State University, Belozersky Institute of Physicochemical Biology and Faculty of Bioengineering and Bioinformatics, Lenin hills 1, bldg. 73, 119991, Moscow, Russia.

In recent years, the phenomenon of allostery has witnessed growing attention driven by a fundamental interest in new ways to regulate the functional properties of proteins, as well as the prospects of using allosteric sites as targets to design novel drugs with lower toxicity due to a higher selectivity of binding and specificity of the mechanism of action. The currently available bioinformatic methods can sometimes correctly detect previously unknown ligand binding sites in protein structures. However, the development of universal and more efficient approaches requires a deeper understanding of the common and distinctive features of the structural organization of both functional (catalytic) and allosteric sites, the evolution of their amino acid sequences in respective protein families, and allosteric communication pathways. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6475866PMC
April 2019
1 Read