461 results match your criteria Acta Naturae[Journal]


Effect of Temperature, pH and Plasmids on In Vitro Biofilm Formation in Escherichia coli.

Acta Naturae 2018 Oct-Dec;10(4):129-132

Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, Laboratory affiliated to the Istituto Pasteur Italia - Fondazione Cenci Bolognetti, Corso della Repubblica 79, 04100 Latina, Italy.

Acid resistance (AR) in is an important trait that protects this microorganism from the deleterious effect of low-pH environments. Reports on biofilm formation in K12 showed that the genes participating in AR were differentially expressed. Herein, we investigated the relationship between AR genes, in particular those coding for specific transcriptional regulators, and their biofilm-forming ability at the phenotypic level. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351026PMC
February 2019
4 Reads

Mouse Model for Assessing the Subchronic Toxicity of Organophosphate Pesticides.

Acta Naturae 2018 Oct-Dec;10(4):125-128

Institute of Bioorganic Chemistry, Academicians M.M. Shemyakin and Yu.A. Ovchinnikova RAS, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia.

The development of antidotes to organophosphate poisons is an important aspect of modern pharmacology. Recombinant acetylcholinesterase and butyrylcholinesterase are effective DNA-encoded acceptors of organophosphate poisons and, in particular, pesticides. Here, we present the results of a study on the effectiveness of recombinant butyrylcholinesterase (BChE) in modeling organophosphate poisoning caused by oral administration of paraoxon at a dose of 2 mg / kg. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351027PMC
February 2019
1 Read

The Preferable Binding Pose of Canonical Butyrylcholinesterase Substrates Is Unproductive for Echothiophate.

Acta Naturae 2018 Oct-Dec;10(4):121-124

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia.

In this paper, we, for the first time, describe the interaction between the butyrylcholinesterase enzyme and echothiophate, a popular model compound and an analogue of the chemical warfare agents VX and VR, at the atomistic level. Competition between the two echothiophate conformations in the active site was found using molecular modeling techniques. The first one is close to the mode of binding of the substrates of choline series (butyrylcholine and butyrylthiocholine) and is inhibitory, since it is unable to react with the enzyme. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351040PMC
February 2019

Tag7-Mts1 Complex Induces Lymphocytes Migration via CCR5 and CXCR3 Receptors.

Acta Naturae 2018 Oct-Dec;10(4):115-120

Institute of Gene Biology of the Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, 119334 , Russia.

The discovery of new chemokines that induce the migration of lymphocytes to the infection site is important for the targeted search for therapeutic agents in immunotherapy. We recently showed that Tag7 (PGLYRP1), an innate immunity protein, forms a stable complex with the Ca -binding protein Mts1 (S100A4), which is able to induce lymphocyte movement, although the individual Tag7 and Mts1 do not have this activity. The purpose of this study is to identify receptors that induce the migration of lymphocytes along the concentration gradient of the Tag7-Mts1 complex, and the components of this complex capable of interacting with these receptors. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351033PMC
February 2019

Zinc Finger Protein CG9890 - New Component of ENY2-Containing Complexes of Drosophila.

Acta Naturae 2018 Oct-Dec;10(4):110-114

Institute of Gene Biology Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, 119334, Russia.

In previous studies, we showed that the insulator protein Su(Hw) containing zinc finger domains interacts with the ENY2 protein and recruits the ENY2-containing complexes on Su(Hw)-dependent insulators, participating in the regulation of transcription and in the positioning of replication origins. Here, we found interaction between ENY2 and CG9890 protein, which also contains zinc finger domains. The interaction between ENY2 and CG9890 was confirmed. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351030PMC
February 2019

Identification of Novel Interaction Partners of AIF Protein on the Outer Mitochondrial Membrane.

Acta Naturae 2018 Oct-Dec;10(4):100-109

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia.

In response to the wide variety of external and internal signals, mammalian cells undergo apoptosis, programmed cell death. Dysregulation of apoptosis is involved in multiple human diseases, including cancer, autoimmunity, and ischemic injuries. Two types of apoptosis have been described: the caspase-dependent one, leading to digestion of cellular proteins, and caspase-independent apoptosis, resulting in DNA fragmentation. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351035PMC
February 2019
2 Reads

The TLR4 Agonist Immunomax Affects the Phenotype of Mouse Lung Macrophages during Respiratory Syncytial Virus Infection.

Acta Naturae 2018 Oct-Dec;10(4):95-99

NRC Institute of Immunology FMBA of Russia, Kashirskoe shosse, 24, Moscow, 115478, Russia.

In the study, the effect of the TLR4 agonist Immunomax was investigated and . In particular, Immunomax was shown to polarize mouse bone marrow macrophages from the M0 and M2 states into the M1 state ( and mRNA expression levels were used to identify the mouse M1 and M2 phenotypes). Next, we investigated the prophylactic antiviral effect of Immunomax in both a model of mouse respiratory syncytial virus (RSV) infection and a model of RSV-induced bronchial asthma (BA) exacerbation. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351037PMC
February 2019
7 Reads

The Mechanism of Fluorescence Quenching of Protein Photosensitizers Based on miniSOG During Internalization of the HER2 Receptor.

Acta Naturae 2018 Oct-Dec;10(4):87-94

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997 , Russia.

The protein photosensitizer miniSOG is a promising agent for photodynamic therapy. The genetically encoded phototoxins 4D5scFv-miniSOG and DARPin-miniSOG specifically bind to the HER2 receptor overexpressed on the surface of cancer cells and promote receptor-mediated internalization of HER2. We show that ingestion of proteins in a complex with the receptor reduces the fluorescent signal of the phototoxic module in endosomes. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351032PMC
February 2019

Variants of Mitochondrial Genome and Risk of Multiple Sclerosis Development in Russians.

Acta Naturae 2018 Oct-Dec;10(4):79-86

Pirogov Russian National Research Medical University, Ostrovitjanova Str. 1, Moscow, 117997, Russia.

For the first time in the history of ethnic Russians, an association analysis the development of multiple sclerosis (MS) was performed for the mitochondrial haplogroups H, J, K, and U, as well as for the individual mitochondrial DNA (mtDNA) polymorphisms discriminating these haplogroups (m.1719G > A, m. 7028C > T, m. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351038PMC
February 2019
7 Reads

Overexpression of Adenoviral E1A Sensitizes E1A+Ras-Transformed Cells to the Action of Histone Deacetylase Inhibitors.

Acta Naturae 2018 Oct-Dec;10(4):70-78

Institute of Cytology, Russian Academy of Sciences, Tikhoretsky Ave., 4, St-Petersburg, 194064, Russia.

The adenoviral E1A protein induces cell proliferation, transformation, and tumor formation in rodents, on the one hand. On the other hand, E1A expression increases cell sensitivity to a number of cytotoxic agents. Therefore, E1A is a candidate for use as a component of combination therapy for malignant tumors. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351034PMC
February 2019

Assessment of the Parameters of Adaptive Cell-Mediated Immunity in Naïve Common Marmosets (Callithrix jacchus).

Acta Naturae 2018 Oct-Dec;10(4):63-69

Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, premises 8, bldg. 1, Village of Institute of Poliomyelitis, Settlement "Moskovskiy", Moscow, 108819, Russia.

Common marmosets are small New World primates that have been increasingly used in biomedical research. This report presents efficient protocols for assessment of the parameters of adaptive cell-mediated immunity in common marmosets, including the major subpopulations of lymphocytes and main markers of T- and B-cell maturation and activation using flow cytometry with a multicolor panel of fluorescently labelled antibodies. Blood samples from eight common marmosets were stained with fluorescently labeled monoclonal antibodies against their population markers (CD45, CD3, CD20, CD4, CD8) and lymphocyte maturation and activation markers (CD69, CD62L, CD45RO, CD107a and CD27) and analyzed by flow cytometry. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351028PMC
February 2019
2 Reads

Gamma-Carbolines Derivatives As Promising Agents for the Development of Pathogenic Therapy for Proteinopathy.

Acta Naturae 2018 Oct-Dec;10(4):59-62

Institute of Physiologically Active Compounds of the Russian Academy of Sciences, Severny Dr., Chernogolovka, 1142432, Russia.

Uncontrolled protein aggregation, accompanied by the formation of specific inclusions, is a major component of the pathogenesis of many common neurodegenerative diseases known as proteinopathies. The intermediate products of this aggregation are toxic to neurons and may be lethal. The development strategy of pathogenic therapy for proteinopathy is based on the design of drugs capable of both inhibiting proteinopathy progression and increasing the survival of affected neurons. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351039PMC
February 2019
4 Reads

"Noah's Ark" Project: Interim Results and Outlook for Classic Collection Development.

Acta Naturae 2018 Oct-Dec;10(4):49-58

M.V. Lomonosov Moscow State University, Leninskie Gory, 1, Moscow, 119991, Russia.

The "Noah's Ark" project, afoot at M.V. Lomonosov Moscow State University since 2015 and aimed at studying biodiversity, is the largest ongoing Russian project in life sciences. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351031PMC
February 2019

Bacterial Enzymes and Antibiotic Resistance.

Acta Naturae 2018 Oct-Dec;10(4):33-48

Chemistry Faculty, M.V. Lomonosov Moscow State University, Leninskie gori, 1, bldg. 3, Moscow, 119991, Russia.

The resistance of microorganisms to antibiotics has been developing for more than 2 billion years and is widely distributed among various representatives of the microbiological world. Bacterial enzymes play a key role in the emergence of resistance. Classification of these enzymes is based on their participation in various biochemical mechanisms: modification of the enzymes that act as antibiotic targets, enzymatic modification of intracellular targets, enzymatic transformation of antibiotics, and the implementation of cellular metabolism reactions. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351036PMC
February 2019

Multifaced Roles of the Urokinase System in the Regulation of Stem Cell Niches.

Acta Naturae 2018 Oct-Dec;10(4):19-32

Laboratory of Angiogenesis, National Medical Research Center of Cardiology, 3rd Cherepkovskaya Str., 15a, Moscow, 121552, Russia.

Proliferation, subsequent migration to the damaged area, differentiation into appropriate cell types, and/or secretion of biologically active molecules and extracellular vesicles are important processes that underlie the involvement of stem/progenitor cells in the repair and regeneration of tissues and organs. All these functions are regulated through the interaction between stem cells and the microenvironment in the tissue cell niches that control these processes through direct cell-cell interactions, production of the extracellular matrix, release of extracellular vesicles, and secretion of growth factors, cytokines, chemokines, and proteases. One of the most important proteolytic systems involved in the regulation of cell migration and proliferation is the urokinase system represented by the urokinase plasminogen activator (uPA, urokinase), its receptor (uPAR), and inhibitors. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351041PMC
February 2019
1 Read

The EIMB Hydrogel Microarray Technology: Thirty Years Later.

Acta Naturae 2018 Oct-Dec;10(4):4-18

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilova Str., 32, Moscow, 119991, Russia.

 Biological microarrays (biochips) are analytical tools that can be used to implement complex integrative genomic and proteomic approaches to the solution of problems of personalized medicine (e.g., patient examination in order to reveal the disease long before the manifestation of clinical symptoms, assess the severity of pathological or infectious processes, and choose a rational treatment). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351029PMC
February 2019

Influence of the Activation of NMDA Receptors on the Resting Membrane Potential of the Postsynaptic Cell at the Neuromuscular Junction.

Acta Naturae 2018 Jul-Sep;10(3):100-102

Open Laboratory of Neuropharmacology, Kazan Federal University, Kremlyovskaya Str., 18, Kazan, 420008, Russia.

Impaired function or insufficient expression of glutamate N-methyl-D-aspartate (NMDA) receptors underlies a number of brain pathologies; these receptors are, therefore, regarded as a pharmacological target for many neuroactive drugs. It was shown that in the CNS, this type of glutamate receptors participate in the processes of neuronal excitation, synaptic plasticity [1, 2], and excitotoxicity in neurodegenerative diseases and are also involved in the pathogenesis of epilepsy and seizures. However, until recently, the presence and activity of NMDA receptors beyond the CNS had never been considered. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209397PMC
November 2018
12 Reads

Expression and Intracellular Localization of Paraoxonase 2 in Different Types of Malignancies.

Acta Naturae 2018 Jul-Sep;10(3):92-99

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia.

PON2 belongs to the paraoxonase protein family that consists of lactone hydrolyzing enzymes with different substrate specificities. Unlike other members of the family, PON2 exhibits substantial antioxidant activity, is localized predominantly inside the cell, and is ubiquitously expressed in all human tissues. Previously, it was proffered that defense against pathogens, such as , is the main function of paraoxonases. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209399PMC
November 2018
17 Reads

A Novel Approach to Anticancer Therapy: Molecular Modules Based on the Barnase:Barstar Pair for Targeted Delivery of HSP70 to Tumor Cells.

Acta Naturae 2018 Jul-Sep;10(3):85-91

Shemyakin - Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia.

One important distinction between many tumor cell types and normal cells consists in the translocation of a number of intracellular proteins, in particular the 70 kDa heat shock protein (HSP70), to the surface of the plasma membrane. It has been demonstrated that such surface localization of HSP70 on tumor cells is recognized by cytotoxic effectors of the immune system, which increases their cytolytic activity. The mechanisms behind this interaction are not fully clear; however, the phenomenon of surface localization of HSP70 on cancer cells can be used to develop new approaches to antitumor immunotherapy. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209404PMC
November 2018
15 Reads

Antidepressant Effect of an Orally Administered Dipeptide Mimetic of the Brain-Derived Neurotrophic Factor.

Acta Naturae 2018 Jul-Sep;10(3):81-84

Federal State Budgetary Institution "Research Zakusov institute of pharmacology", Baltic Str., 8, Moscow, 125315, Russia.

Involvement of BDNF in the regulation of neuroplasticity and neurogenesis in the hippocampus, impairment of which underlies the pathophysiology of depression, makes this endogenous protein a promising object for the development of new-generation antidepressants with a neurophysiologically based mechanism of action. A low-molecular-weight BDNF mimetic, GSB-106 (a substituted dimeric dipeptide, bis-(N-monosuccinyl- L-seryl-L-lysine) hexamethylenediamide), was designed and synthesized at the Zakusov Institute of Pharmacology. GSB-106 was found to activate BDNF-specific TrkB receptors and their main post-receptor signaling pathways MAPK/ERK and PI3K/AKT. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209401PMC
November 2018
15 Reads

Antibacterial Effect of Thiosulfinates on Multiresistant Strains of Bacteria Isolated from Patients with Cystic Fibrosis.

Acta Naturae 2018 Jul-Sep;10(3):77-80

Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences, Vavilova Str., 32, Moscow, 119991, Russia.

The multiresistance of 155B, 122, and 48B strains isolated from patients with cystic fibrosis was established. The antibacterial effect of allicin, dimethyl thiosulfinate, and dipropyl thiosulfinate on multidrug-resistant strains was shown. Thiosulfinates can have both bacteriostatic and bactericidal effects depending on the microorganism and the concentration. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209396PMC
November 2018
1 Read

Whole-Genome Sequencing of Russian Neisseria Gonorrhoeae Isolates Related to ST 1407 Genogroup.

Acta Naturae 2018 Jul-Sep;10(3):68-76

State Research Centre of Dermatovenerology and Cosmetology, Korolenko Str., 3/6, Moscow, 107076 , Russia.

The whole-genome sequencing data of three strains isolated in the Russian Federation in 2015 are presented. According to the NG-MAST protocol, these strains are related to the globally spread ST 1407 genogroup. The analysis of their resistomes showed the absence of genes and the presence of wild-type alleles of and genes, and these patterns explain the susceptibility of the sequenced strains to aminocyclitols (spectinomycin) and macrolides (azithromycin). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209400PMC
November 2018
1 Read

Biochemical and Functional Changes in the Eye As a Manifestation of Systemic Degeneration of the Nervous System in Parkinsonism.

Acta Naturae 2018 Jul-Sep;10(3):62-67

Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Vavilov Str., 26, Moscow, 119334, Russia.

Parkinson's disease (PD) is a systemic neurodegenerative condition caused by the death of dopaminergic neurons of the nigrostriatal system of the brain. This disease is diagnosed after most neurons have already been lost, which explains the low efficiency of treatment. Hope for increasing treatment efficiency rests in the development of new strategies for early diagnosis of PD based on a search for peripheral markers that appear as early changes in non-motor functions. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209403PMC
November 2018
4 Reads

Lynx1 Prevents Long-Term Potentiation Blockade and Reduction of Neuromodulator Expression Caused by Aβ1-42 and JNK Activation.

Acta Naturae 2018 Jul-Sep;10(3):57-61

Lomonosov Moscow State University, Leninskie Gori 1, Moscow, 119234, Russia.

Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels. Many neurodegenerative diseases are accompanied by cognitive impairment associated with the dysfunction of nAChRs. The human membrane-tethered prototoxin Lynx1 modulates nAChR function in the brain areas responsible for learning and memory. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209405PMC
November 2018
4 Reads

Three-Dimensional Structure of Cytochrome c Nitrite Reductase As Determined by Cryo-Electron Microscopy.

Acta Naturae 2018 Jul-Sep;10(3):48-56

National Research Center «Kurchatov Institute», Akademika Kurchatova Sqr., 1, Moscow, 123182 , Russia.

The structure of cytochrome c nitrite reductase from the bacterium Thioalkalivibrio nitratireducens was determined by cryo-electron microscopy (cryo-EM) at a 2.56 Å resolution. Possible structural heterogeneity of the enzyme was assessed. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209398PMC
November 2018

Production of Recombinant Proteins in the Milk of Transgenic Animals: Current State and Prospects.

Acta Naturae 2018 Jul-Sep;10(3):40-47

Institute of Gene Biology, Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, 119334, Russia.

The use of transgenic animals as bioreactors for the synthesis of the recombinant proteins secreted into milk is a current trend in the development of biotechnologies. Advances in genetic engineering, in particular the emergence of targeted genome editing technologies, have provided new opportunities and significantly improved efficiency in the generation of animals that produce recombinant proteins in milk, including economically important animals. Here, we present a retrospective review of technologies for generating transgenic animals, with emphasis on the creation of animals that produce recombinant proteins in milk. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209402PMC
November 2018
6 Reads

Possibilities for Using Pluripotent Stem Cells for Restoring Damaged Eye Retinal Pigment Epithelium.

Acta Naturae 2018 Jul-Sep;10(3):30-39

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Malaya Pirogovskaya Str., 1a, Moscow, 119435, Russia.

The retinal pigment epithelium is a monolayer of pigmented, hexagonal cells connected by tight junctions. These cells compose part of the outer blood-retina barrier, protect the eye from excessive light, have important secretory functions, and support the function of photoreceptors, ensuring the coordination of a variety of regulatory mechanisms. It is the degeneration of the pigment epithelium that is the root cause of many retinal degenerative diseases. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209409PMC
November 2018
17 Reads

High-Throughput Screening of Biodiversity for Antibiotic Discovery.

Acta Naturae 2018 Jul-Sep;10(3):23-29

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia.

The increasing number of infections caused by antibiotic-resistant strains of pathogens challenges modern technologies of drug discovery. Combinatorial chemistry approaches are based on chemical libraries. They enable the creation of high-affinity low-molecular-weight ligands of the therapeutically significant molecular targets of human cells, thus opening an avenue toward a directed design of highly effective therapeutic agents. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209406PMC
November 2018
26 Reads

B Cell Regulation in Autoimmune Diseases.

Acta Naturae 2018 Jul-Sep;10(3):11-22

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia.

Antibody-independent B cell effector functions play an important role in the development and suppression of the immune response. An extensive body of data on cytokine regulation of the immune response by B lymphocytes has been accumulated over the past fifteen years. In this review, we focused on the mechanisms of inflammatory response suppression by subpopulations of regulatory B cells in health and autoimmune pathologies. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209408PMC
November 2018
8 Reads

Experimental Models of Spinal Cord Injury in Laboratory Rats.

Acta Naturae 2018 Jul-Sep;10(3):4-10

Branch of Shemyakin and Ovchinnikov institute of bioorganic chemistry Russian academy of sciences, Prospekt Nauki, 6, Moscow region, Pushchino, 142290, Russia.

Pathologies associated with spinal cord injury are some of the leading diseases in the world. The search for new therapeutic agents and 3D biodegradable materials for the recovery of spinal cord functions is a topical issue. In this review, we have summarized the literature data on the most common experimental models of spinal cord injury in laboratory rats and analyzed the experience of using 3D biodegradable materials (scaffolds) in experimental studies of spinal trauma. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209407PMC
November 2018
7 Reads

Role of the Scaffold Protein MIM in the Actin-Dependent Regulation of Epithelial Sodium Channels (ENaC).

Acta Naturae 2018 Apr-Jun;10(2):97-103

Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.

Epithelial Sodium Channels (ENaCs) are expressed in different organs and tissues, particularly in the cortical collecting duct (CCD) in the kidney, where they fine tune sodium reabsorption. Dynamic rearrangements of the cytoskeleton are one of the common mechanisms of ENaC activity regulation. In our previous studies, we showed that the actin-binding proteins cortactin and Arp2/3 complex are involved in the cytoskeleton-dependent regulation of ENaC and that their cooperative work decreases a channel's probability of remaining open; however, the specific mechanism of interaction between actin-binding proteins and ENaC is unclear. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6087825PMC
August 2018
11 Reads

ATP Reduces the Entry of Calcium Ions into the Nerve Ending by Blocking L-type Calcium Channels.

Acta Naturae 2018 Apr-Jun;10(2):93-96

Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, P.O. Box 30, Kazan, 420111, Russia.

At neuromuscular junctions, ATP inhibits both the evoked and spontaneous acetylcholine release and inward calcium current operating via presynaptic P2Y receptors. It was shown in the experiments with the frog neuromuscular synapse using specific calcium-sensitive dye Oregon Green Bapta 1 that exogenous ATP reduces the amplitude of calcium transient, which reflects the changes in the entry of calcium ions in response to the nerve pulse. The depressing effect of ATP on the transient was prevented by suramin, the blocker of P2 receptors. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6087822PMC
August 2018
13 Reads

Phylogenetic Analysis and Molecular Typing of Trichothecene-Producing Fusarium Fungi from Russian Collections.

Acta Naturae 2018 Apr-Jun;10(2):79-92

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic chemistry of the Russian Academy of Sciences, Miklukho-Maklaya Str. 16\10, Moscow, 117997, Russia.

We performed a three-locus phylogenetic analysis of strains presumably capable of trichothecene production, which were deposited in the Russian national collections. The intra- and interspecific polymorphism of partial sequences of the translation elongation factor 1 alpha () gene and two genes from the trichothecene cluster and was studied. A study of 60 strains of different origins using DNA markers confirmed, and in the case for several strains, clarified their taxonomic characteristics. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6087817PMC
August 2018
1 Read

Soluble Guanylate Cyclase As the Key Enzyme in the Modulating Effect of NO on Metabotropic Glutamate Receptors.

Acta Naturae 2018 Apr-Jun;10(2):71-78

Pavlov Institute of Physiology, Russian Academy of Sciences, Makarova Embankment 6, St. Petersburg, 199034, Russia.

The synaptic plasticity of the afferent synapse of the vestibular apparatus is defined by the dynamic interaction of ionotropic and metabotropic glutamate receptors and the modulators of synaptic transmission. It was shown that nitric oxide modulates iGluR responses. In this paper, the effect of NO on the function of the afferent synapse mGluR was investigated. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6087819PMC
August 2018
8 Reads

Reversible Cyclic Thermal Inactivation of Oligopeptidase B from Serratia proteamaculans.

Acta Naturae 2018 Apr-Jun;10(2):65-70

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16/10, Moscow, 117997, Russia.

A unique property was found for oligopeptidase B from (PSP) as well as its mutants: they can undergo reversible thermal inactivation at 37°C, with activity being restored or even increased with respect to the initial one upon subsequent cooling. The process can be repeated several times, with the same results achieved (up to 5 cycles). This effect can be explained by a shift in the equilibrium between the inactive open form of the enzyme and the active closed one upon variation of the incubation temperature. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6087823PMC
August 2018
1 Read

1-(4-Phenoxybenzyl) 5-Aminouracil Derivatives and Their Analogues - Novel Inhibitors of Human Adenovirus Replication.

Acta Naturae 2018 Apr-Jun;10(2):58-64

N.F. Gamaleya Federal National Research Center for Epidemiology and Microbiology, Gamaleya Str. 18, Moscow, 123098, Russia.

Adenovirus infections are characterized by widespread distribution. The lack of causal therapy, which is effective in treating this group of diseases, explains the need for new therapeutic drugs. Notably, anti-adenoviral activity of [4-(phenoxy)benzyl]-5-(phenylamino)-6-azauracil, 1-[4-(phenoxy)benzyl]-5-(morpholino) uracil, 1-[4-(4-chlorophenoxy)benzyl]-5-(morpholino) uracil, and 1-[4-(4-fluorophenoxy)-benzyl]-5-(morpholino) uracil was observed. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6087815PMC

Characterization of the T-cell Repertoire after Autologous HSCT in Patients with Ankylosing Spondylitis.

Acta Naturae 2018 Apr-Jun;10(2):48-57

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya Str. 16/10, Moscow, 117997, Russia.

Autologous hematopoietic stem cell transplantation (HSCT), a safer type of HSCT than allogeneic HSCT, is a promising therapy for patients with severe autoimmune diseases (ADs). Despite the long history of medical practice, structural changes in the adaptive immune system as a result of autologous HSCT in patients with various types of ADs remain poorly understood. In this study, we used high-throughput sequencing to investigate the structural changes in the peripheral blood T-cell repertoire in adult patients with ankylosing spondylitis (AS) during two years after autologous HSCT. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6087820PMC
August 2018
11 Reads

Cognitive Tagging of Neurons: CREMediated Genetic Labeling and Characterization of the Cells Involved in Learning and Memory.

Acta Naturae 2018 Apr-Jun;10(2):37-47

Department of Neuroscience, National Research Center "Kurchatov Institute", Akademika Kurchatova pl. 1, Moscow, 123182, Russia.

In this study, we describe use of Cre-mediated recombination to obtain a permanent genetic labeling of the brain neuronal networks activated during a new experience in animals. This method utilizes bitransgenic Fos-Cre-eGFP mice in which a green fluorescent protein is expressed upon tamoxifen-induced Cre-recombination only in the cells where immediate early gene expression takes place due to the new experience. We used the classical fear conditioning model to show that microscopy of the eGFP protein in Fos-Cre-eGFP mice enables mapping of the neurons of the various brain regions that undergo Cre-recombination during acquisition of a new experience. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6087816PMC
August 2018
2 Reads

The Development and Study of Recombinant Immunoglobulin A to Hemagglutinins of the Influenza Virus.

Acta Naturae 2018 Apr-Jun;10(2):30-36

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16/10, Moscow, 117997, Russia.

We obtained recombinant variants of human antibody FI6 broadly specific to hemagglutinins of the influenza A virus. On the basis of a bi-promoter (CMV, hEF1-HTLV) vector, we developed genetic constructs for the expression of the heavy and light chains of the immunoglobulins of IgA1-, IgA2m1-, and IgG-isotypes. Following transfection and selection, stable Chinese hamster ovary (CHO) cell lines were produced. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6087826PMC
August 2018
2 Reads

Organism-Level Tumor Models in Zebrafish Danio rerio.

Acta Naturae 2018 Apr-Jun;10(2):24-29

Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov Sq., 2, Moscow, 123182 , Russia.

Development and implementation of adequate organism-level models is one of the key elements in biomedical research that focuses on experimental oncology. Over the last decade, studies using Zebrafish () have gained in popularity in this area of research. This review describes the various approaches that have been used in developing highly effective models for oncological (clinical term, better cancer or tumor) studies based on . Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6087818PMC

Molecular Approaches to Safe and Controlled Engineered T-cell Therapy.

Acta Naturae 2018 Apr-Jun;10(2):16-23

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16 /10, Moscow, 117997, Russia.

Chimeric antigen receptor-modified T-cell therapy (CAR-T therapy) is one of the fastest developing areas of immuno-oncology. Over the past decade, it has revolutionized the cell therapy modality and expedited its pace of development, from optimization of the structure of chimeric antigen receptors and animal model experiments to successful clinical application. The initial designs of the CAR configuration focused on increasing T-cell activation, cytotoxicity, and persistence. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6087824PMC
August 2018
14 Reads

Hypertension and Cerebral Microangiopathy (Cerebral Small Vessel Disease): Genetic and Epigenetic Aspects of Their Relationship.

Acta Naturae 2018 Apr-Jun;10(2):4-15

Research center of neurology, Volokolamskoe Shosse 80, Moscow, 125367, Russia.

Hypertension (HT) and its cerebral complications are extremely vexing medical and social problems. Despite the obvious association between hypertension and the clinical and neuroimaging features of cerebral microangiopathy (CMA) (also known as cerebral small vessel disease), the causal links between them remain ambiguous. Besides, antihypertensive therapy as the only way to manage these patients does not always prevent brain damage. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6087821PMC

Influence of the Linking Order of Fragments of HA2 and M2e of the influenza A Virus to Flagellin on the Properties of Recombinant Proteins.

Acta Naturae 2018 Jan-Mar;10(1):85-94

Research Institute of Influenza, Russian Federation Ministry of Health, Prof. Popova Str.15/17, St. Petersburg, 197376, Russia.

The ectodomain of the M2 protein (M2e) and the conserved fragment of the second subunit of hemagglutinin (HA2) are promising candidates for broadly protective vaccines. In this paper, we report on the design of chimeric constructs with differing orders of linkage of four tandem copies of M2e and the conserved fragment of HA2 (76-130) from phylogenetic group II influenza A viruses to the C-terminus of flagellin. The 3D-structure of two chimeric proteins showed that interior location of the M2e tandem copies (Flg-4M2e-HA2) provides partial α-helix formation nontypical of native M2e on the virion surface. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5916737PMC

New Experimental Models of Retinal Degeneration for Screening Molecular Photochromic Ion Channel Blockers.

Acta Naturae 2018 Jan-Mar;10(1):75-84

Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Toreza Ave. 44, St. Petersburg, 194223, Russia.

Application of molecular photochromic ion channel blockers to recover the visual function of a degenerated retina is one of the promising trends in photopharmacology. To this day, several photochromic azobenzene-based compounds have been proposed and their functionality has been demonstrated on cell lines and knockout mouse models. Further advance necessitates testing of the physiological activity of a great number of new compounds. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5916736PMC

Gene Expression in the Three-Spined Stickleback (Gasterosteus aculeatus) of Marine and Freshwater Ecotypes.

Acta Naturae 2018 Jan-Mar;10(1):66-74

National Research Center "Kurchatov Institute", Kurchatov Sq. 1, Moscow, 123182, Russia.

Three-spine stickleback is a well-known model organism that is routinely used to explore microevolution processes and speciation, and the number of studies related to this fish has been growing recently. The main reason for the increased interest is the processes of freshwater adaptation taking place in natural populations of this species. Freshwater three-spined stickleback populations form when marine water three-spined sticklebacks fish start spending their entire lifecycle in freshwater lakes and streams. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5916735PMC
May 2018
2 Reads

A Highly Productive CHO Cell Line Secreting Human Blood Clotting Factor IX.

Acta Naturae 2018 Jan-Mar;10(1):51-65

Institute of Bioengineering of the Federal Research Centre "Fundamentals of Biotechnology" of the Russian Academy of Sciences, 60-let Oktjabrja Ave. 7, bldg. 1, Moscow, 117312, Russia.

Hemophilia B patients suffer from an inherited blood-clotting defect and require regular administration of blood-clotting factor IX replacement therapy. Recombinant human factor IX produced in cultured CHO cells is nearly identical to natural, plasma-derived factor IX and is widely used in clinical practice. Development of a biosimilar recombinant human factor IX for medical applications requires the generation of a clonal cell line with the highest specific productivity possible and a high level of specific procoagulant activity of the secreted factor IX. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5916734PMC
May 2018
6 Reads

The PTENP1 Pseudogene, Unlike the PTEN Gene, Is Methylated in Normal Endometrium, As Well As in Endometrial Hyperplasias and Carcinomas in Middle-Aged and Elderly Females.

Acta Naturae 2018 Jan-Mar;10(1):43-50

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Miklukho- Maklaya Str. 16/10, Moscow, 117997, Russia.

The tumor suppressor PTEN controls multiple cellular functions, including cell cycle, apoptosis, senescence, transcription, and mRNA translation of numerous genes. In tumor cells, PTEN is frequently inactivated by genetic mutations and epimutations. The aim of this study was to investigate the methylation patterns of the gene and its pseudogene as potential genetic markers of endometrial hyperplasia (EH) and endometrial carcinoma (EC). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5916733PMC

The Effect of TNF and VEGF on the Properties of Ea.hy926 Endothelial Cells in a Model of Multi-Cellular Spheroids.

Acta Naturae 2018 Jan-Mar;10(1):34-42

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16/10, Moscow, 117997, Russia.

Endothelial cells play a major role in the development of inflammation and neoangiogenesis in cancer and chronic inflammatory diseases. In 3D cultures, cells are under conditions that closely resemble those existing in healthy and disease-stricken human organs and tissues. Therefore, the development of a 3D model based on the Ea. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5916732PMC

Pathogenesis of Type 1 Diabetes Mellitus and Rodent Experimental Models.

Acta Naturae 2018 Jan-Mar;10(1):24-33

Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova Str. 26, Moscow, 119334, Russia.

The global prevalence of diabetes mellitus and its severe complications is on the rise. The study of the pathogenesis of the onset and the progression of complications related to the disease, as well as the search for new therapeutic agents and methods of treatment, remains relevant. Experimental models are extremely important in the study of diabetes. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5916731PMC

The Role of TAL1 in Hematopoiesis and Leukemogenesis.

Acta Naturae 2018 Jan-Mar;10(1):15-23

The Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilova Str. 32, Moscow,119991, Russia.

TAL1 (SCL/TAL1, T-cell acute leukemia protein 1) is a transcription factor that is involved in the process of hematopoiesis and leukemogenesis. It participates in blood cell formation, forms mesoderm in early embryogenesis, and regulates hematopoiesis in adult organisms. TAL1 is essential in maintaining the multipotency of hematopoietic stem cells (HSC) and keeping them in quiescence (stage G0). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5916730PMC
May 2018
4 Reads