703,880 results match your criteria Acta Microscopica[Journal]


Antifouling ionic liquid doped molecularly imprinted polymer-based ratiometric electrochemical sensor for highly stable and selective detection of zearalenone.

Anal Chim Acta 2022 Jun 30;1210:339884. Epub 2022 Apr 30.

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, Hubei Province, PR China. Electronic address:

Zearalenone (ZEN) is a nonsteroidal estrogenic mycotoxin, and its accurate detection in complex biological samples is still a challenge. Herein, an antifouling ratiometric electrochemical sensor has been developed for its detection. In this system, black phosphorus-graphene oxide is used as substrate to amplify the signal; ionic liquid doped molecularly imprinted polymer (MIP) endows the sensing surface not only high recognition ability but also high capability to resist nonspecific adsorption. Read More

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Quenching of tungsten-based polyoxometalate nanoclusters on electrochemiluminescence emission of luminol loaded CeVO/Au for immunoassay of protein.

Anal Chim Acta 2022 Jun 29;1210:339883. Epub 2022 Apr 29.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China. Electronic address:

A quenching-typed electrochemiluminescence (ECL) immunosensing method was proposed for protein detection by the redox reaction of tungsten-based polyoxometalate nanoclusters (W-POM NCs) with free radical (O) as the intermediate of co-reactant HO. The immunosensor was constructed by co-immobilizing luminol loaded CeVO/Au nanohybrids and capture antibody on an indium tin oxide electrode, which showed strong ECL emission in the presence of HO due to the catalysis of CeVO/Au toward its electro-oxidation to produce O via the reversible conversion between Ce and Ce. The W-POM NCs were encapsulated in the uniformly sized amino-silica nanosphere (SiO) to act as a label ([email protected]) of the secondary antibody. Read More

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Sweat urea bioassay based on degradation of Prussian Blue as the sensing architecture.

Anal Chim Acta 2022 Jun 29;1210:339882. Epub 2022 Apr 29.

Department of Pharmacy, University of Naples "Federico II", Via D. Montesano 49, 80131, Naples, Italy; BAT Center - Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology, University of Naples Federico II, 80055, Naples, Italy. Electronic address:

Urea provides pathophysiological information on renal and hepatic disorders. Among the secretion pathways via several bio-fluids, sweat urea represent an important and non-invasive strategy to evaluate kidney and liver pathologies. In this work, a rapid and reliable colorimetric urea bioassay assisted by chemometrics design of experiments has been obtained. Read More

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pH-guided fluorescent sensing probe for the discriminative detection of Cl and Br in human serum.

Anal Chim Acta 2022 Jun 27;1210:339879. Epub 2022 Apr 27.

Department of Chemistry, Gwangju Institute of Science and Technology, 123 Cheomdangwagi-ro, Buk-gu, Gwangju, 61005, Republic of Korea. Electronic address:

The fluctuation of the halide ion concentration in human serum is of a high clinical implication. The measurement of Cl in human serum samples is important because Cl is a major constituent of human serum and certain Cl levels are indicators of many acute diseases. Real-time monitoring of Br levels in human serum samples from patients ingesting Br salt-based antiepileptic drugs is important to regulate potential adverse drug effects. Read More

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Quantification of immunosuppressants from one 3.2 mm dried blood spot by a novel cold-induced phase separation based LC-MS/MS method.

Anal Chim Acta 2022 Jun 1;1210:339889. Epub 2022 May 1.

Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China. Electronic address:

Dried blood spots (DBS) have been regarded as a promising alternative for therapeutic drug monitoring (TDM) of immunosuppressants (ISDs) for over fifteen years. Nonetheless, there are still three main issues impeding its preference: (i) the requirement of relatively large disc; (ii) the controversial and intricate desorption approaches; (iii) the lack of feasible extraction strategies. For improvement, this work described a new LC-MS/MS method realizing quantification of four ISDs from one piece of 3. Read More

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A novel UHPLC-ESI-MS/MS method and automatic calculation software for regiospecific analysis of triacylglycerols in natural fats and oils.

Anal Chim Acta 2022 Jun 1;1210:339887. Epub 2022 May 1.

Food Chemistry and Food Development, Department of Life Technologies, FI-20500, University of Turku, Turku, Finland. Electronic address:

Regioisomeric analysis of triacylglycerols (TAGs) in natural oils and fats is a highly challenging task in analytical chemistry. Here we present a software (TAG Analyzer) for automatic calculation of regioisomeric composition of TAGs based on the mass spectral data from recently reported ultra-high performance liquid chromatography electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) method for analyzing TAG regioisomers. The software enables fast and accurate processing of complex product ion spectra containing structurally informative diacylglycerol [M+NH-RCOH-NH] and fatty acid ketene [RCO] fragment ions. Read More

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Trapped ion mobility spectrometry-mass spectrometry improves the coverage and accuracy of four-dimensional untargeted lipidomics.

Anal Chim Acta 2022 Jun 2;1210:339886. Epub 2022 May 2.

Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, 200032, PR China. Electronic address:

Lipids play vital roles in many physiological and pathological processes in living organisms. Due to the high structural diversity and the numerous isomers and isobars of lipids, high-coverage and high-accuracy lipidomic analysis of complex biological samples remain the bottleneck to investigate lipid metabolism. Here, we developed the trapped ion mobility spectrometry-mass spectrometry (TIMS-MS) based four-dimensional untargeted lipidomics to support accurate lipid identification and quantification in biological samples. Read More

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Quantification of endocannabinoids in human cerebrospinal fluid using a novel micro-flow liquid chromatography-mass spectrometry method.

Anal Chim Acta 2022 Jun 2;1210:339888. Epub 2022 May 2.

Division of Systems Biomedicine and Pharmacology, Leiden Academic Centre for Drug Research, Leiden University, Leiden, the Netherlands. Electronic address:

The endocannabinoid system (ECS) is implicated in various brain disorders. Changes in the composition of the cerebrospinal fluid (CSF) may be associated with ECS-related pathologies. Endocannabinoids (eCBs) and their analogues are present at low concentrations in human CSF, which hampered the investigation of the ECS in this body fluid. Read More

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Multicommutation flow analysis system for non-enzymatic lactate determination based on light-driven photometric assay.

Anal Chim Acta 2022 Jun 27;1210:339878. Epub 2022 Apr 27.

University of Warsaw, Faculty of Chemistry, Pasteura 1, 02-093, Warsaw, Poland. Electronic address:

A fully-mechanized bioanalytical system for the photometric light-driven lactate determination is presented. The system is based on highly integrated four diode flow-through cell in which two UV-LEDs are used for the photochemical reduction of Fe(III) in the presence of lactate whereas LED-photodiode system allows the photometric detection of Fe(II)-Ferrozine complex to be registered. The developed multicommutation flow analysis system consisting of bifunctional optoelectronic device (photodegradation of Fe(III)-lactate complex and detection), solenoid micropumps and microvalves, is fully controlled and powered by the Arduino-compatible electronics. Read More

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Renewable sorbent dispersive solid phase extraction automated by Lab-In-Syringe using magnetite-functionalized hydrophilic-lipophilic balanced sorbent coupled online to HPLC for determination of surface water contaminants.

Anal Chim Acta 2022 Jun 26;1210:339874. Epub 2022 Apr 26.

Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, Hradec Králové, 500 05, Czech Republic. Electronic address:

An automated methodology for magnetic dispersive solid phase microextraction integrating bead injection approach for renewable sorbent introduction is presented for the first time and was successfully applied to the enrichment of water contaminants. For this purpose, a simple procedure was developed for the functionalization of commercial SupelTM-Select HLB (Hydrophilic modified styrene polymer) sorbent beads that allowed embedding magnetite nanoparticles (FeO). The sorbent was then used in a dispersive solid phase extraction procedure that was carried out entirely inside the void of an automatic syringe pump following the flow-batch concept of Lab-In-Syringe including automated renewal of the sorbent for each analysis. Read More

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BSA-Cu(PO) hybrid nanoflowers as a high-performance redox indicator for robust label-free electrochemical immunoassay.

Anal Chim Acta 2022 Jun 26;1210:339873. Epub 2022 Apr 26.

Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi, 710127, PR China. Electronic address:

In this work, we report that copper ion-centered hybrid nanoflowers (HNFs) can directly act as high-performance redox indicator via a facile one-step coprecipitation methodology. By altering the bovine serum albumin (BSA) concentration in the coprecipitation synthesis system, the nucleation, growth and electrochemical activities of BSA-Cu(PO) HNFs could be well regulated. Thanks to the intrinsic redox activity and high specific surface area of BSA-Cu(PO) HNFs, it could be utilized not only as the direct electrochemical signal indicator, but also acted as nanocarriers for loading numerous detection antibodies, which was further employed for robust label-free electrochemical immunoassay of α-fetoprotein (AFP). Read More

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A two-dimensional G-CoP/N,P-co-doped carbon nanowire electrode for the simultaneous determination of hydroquinone and catechol in domestic wastewater.

Anal Chim Acta 2022 Jun 28;1210:339871. Epub 2022 Apr 28.

Key Laboratory of Magnetic Molecules and Magnetic Information Materials (Ministry of Education), School of Chemistry and Material Science, Shanxi Normal University, Taiyuan, 030031, China. Electronic address:

Hydroquinone (HQ) and catechol (CC) are important chemical raw materials in the modern industry, unfortunately, which are also high toxic phenolic pollutants. So how to achieve highly sensitive and selective determination HQ and CC is the challenge we face. In the present work, we report a facile strategy to obtain nitrogen and phosphorous co-doped glucose-derived carbon coated CoP nanowires (G-CoP/N,P-C NWs), in which nitrilotriacetic acid (NTA) was as the chelating reagent, glucose was as carbon source, and the precursors were subsequently experienced carbonization and phosphorization process. Read More

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Au-Se bonded nanoprobe for prostate specific antigen detection in serum.

Anal Chim Acta 2022 Jun 25;1210:339852. Epub 2022 Apr 25.

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan, 250014, PR China. Electronic address:

The detection of prostate specific antigen (PSA) in serum can realize early diagnosis of prostate cancer and prevent the occurrence of prostate tumors, as well as offering guidance during the therapy. Herein, a Au-Se bonded nanoprobes that can specifically detect PSA was designed and constructed. The peptide chains that can be specifically cleaved by PSA were firstly functionalized with fluorescent dye and selenol, and then bind to the Au nanoparticles to produce the probe. Read More

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Low and high resolution gas chromatography-mass spectrometry for untargeted metabolomics: A tutorial.

Anal Chim Acta 2022 Jun 9;1210:339043. Epub 2021 Sep 9.

Centre for Metabolomics and Bioanalysis (CEMBIO), Faculty of Pharmacy, Universidad San Pablo CEU, CEU Universities. Campus Monteprincipe, Boadilla Del Monte, 28668, Madrid, Spain. Electronic address:

GC-MS for untargeted metabolomics is a well-established technique. Small molecules and molecules made volatile by derivatization can be measured and those compounds are key players in main biological pathways. This tutorial provides ready-to-use protocols for GC-MS-based metabolomics, using either the well-known low-resolution approach (GC-Q-MS) with nominal mass or the more recent high-resolution approach (GC-QTOF-MS) with accurate mass, discussing their corresponding strengths and limitations. Read More

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Tumor microenvironment-responsive versatile "Trojan horse" theranostic nanoplatform for magnetic resonance imaging-guided multimodal synergistic antitumor treatment.

Acta Biomater 2022 May 17. Epub 2022 May 17.

School of Pharmacy, Xuzhou Medical University, Xuzhou, 221004, China; Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, 221004, PR China. Electronic address:

A natural killer (NK)-92 cell membrane-camouflaged mesoporous MnO-enveloped [email protected] ([email protected]@MnO) nanoparticles (denoted as APMN NPs)-based versatile biomimetic theranostic nanoplatform was developed for magnetic resonance (MR) imaging-guided multimodal synergistic antitumor treatments. In this core-shell nanostructure, an [email protected] core induced near-infrared (NIR)-activatable hyperthermal effects and nanozyme catalytic activity, while a mesoporous MnO shell not only afforded a high drug-loading capability, tumor microenvironment (TME)-triggered MR imaging and drug release, but also endowed catalase-, glutathione peroxidase-, and Fenton-like activities. Furthermore, the NK-92 cell membrane camouflaging endowed the NPs with enhanced tumor-targeting capability, immune escape function, and membrane protein-mediated tumoral uptake property. Read More

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Osteoblastic protein kinase D1 contributes to the prostate cancer cells dormancy via GAS6-circadian clock signaling.

Biochim Biophys Acta Mol Cell Res 2022 May 17:119296. Epub 2022 May 17.

Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China. Electronic address:

Disseminated prostate cancer (PCa) is known to have a strong propensity for bone marrow. These disseminated tumor cells (DTCs) can survive in bone marrow for years without obvious proliferation, while maintaining the ability to develop into metastatic lesions. However, how DTCs kept dormant and recur is still uncertain. Read More

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Analysis of immune response in BALB/c mice immunized with recombinant plasmids pMZ-X3-Ts14-3-3.3 and pMZ-X3-sp-Ts14-3-3.3 of Taenia solium.

Acta Trop 2022 May 17:106517. Epub 2022 May 17.

Department of Parasitology, Zunyi Medical University, Zunyi 563000, China. Electronic address:

There is a lack of vaccine against human cysticercosis, thus making a huge population at the risk of infection. In this study, we chose a novel potential antigen molecule Taenia solium 14-3-3.3 (Ts14-3-3. Read More

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Mapping linear B-cell epitopes of the Tryparedoxin Peroxidase and its implications in the serological diagnosis of tegumentary leishmaniasis.

Acta Trop 2022 May 17:106521. Epub 2022 May 17.

Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Departamento de Patologia Clínica, COLTEC, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. Electronic address:

Diagnosis of tegumentary leishmaniasis (TL) is essential to avoid permanent damage and severe functional sequelae and there is an urgent need to discover new antigens. The present study aimed to comprehensively evaluate the potential use of the Tryparedoxin Peroxidase (TryP) as an antigen for serological tests. The proposal integrates data from immunoproteomics with immunoinformatics, in addition to a precise analysis of protein levels in the evolutionary stages of the parasite by flow cytometry. Read More

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Type I collagen reduces lipid accumulation during adipogenesis of preadipocytes 3T3-L1 via the YAP-mTOR-autophagy axis.

Biochim Biophys Acta Mol Cell Biol Lipids 2022 May 17:159181. Epub 2022 May 17.

Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, Liaoning, China; Key Laboratory of Computational Chemistry-Based Natural Antitumor Drug Research & Development Liaoning Province, Liaoning, China. Electronic address:

The extracellular matrix (ECM) regulates cell behavior through signal transduction and provides a suitable place for cell survival. As one of the major components of the extracellular matrix, type I collagen is involved in regulating cell migration, proliferation and differentiation. We present a system in which 3T3-L1 preadipocyte cells are induced for adipogenic differentiation on type I collagen coated dishes. Read More

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Evaluation of the context of downstream N- and free N-glycomic alterations induced by swainsonine in HepG2 cells.

Biochim Biophys Acta Gen Subj 2022 May 17:130168. Epub 2022 May 17.

Department of Pharmacy, Kinjo Gakuin University, Nagoya 463-8521, Japan. Electronic address:

Swainsonine (SWA), a potent inhibitor of class II α-mannosidases, is present in a number of plant species worldwide and causes severe toxicosis in livestock grazing these plants. The mechanisms underlying SWA-induced animal poisoning are not fully understood. In this study, we analyzed the alterations that occur in N- and free N-glycomic upon addition of SWA to HepG2 cells to understand better SWA-induced glycomic alterations. Read More

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Defects and countermeasures in laboratory diagnosis of rare IgE multiple myeloma.

Clin Chim Acta 2022 May 17. Epub 2022 May 17.

Department of Central Laboratory, Affiliated Hangzhou first people's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310006, China.

Background: IgE multiple myeloma (MM) is a rare subtype of MM that is easily misdiagnosed. We report a rare case of IgE-MM and investigate the application of the SLiM-CRAB criteria to screen for high-risk smoldering MM (SMM) patients, so as to summarize the causes and methods used to prevent missed diagnosis or misdiagnosis in IgE-MM.

Methods: The serum monoclonal protein (M-protein) classification and IgE quantification was performed and sent to several individual institutions. Read More

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Sigma Metrics in Laboratory Medicine: A Call for Harmonization.

Clin Chim Acta 2022 May 17. Epub 2022 May 17.

Department of Laboratory Medicine, Clinique St-Luc Bouge, Namur, Belgium; Department of Pharmacy, Namur Research Institute for LIfe Sciences, University of Namur, Namur, Belgium. Electronic address:

Background And Aim: Sigma metrics are applied in clinical laboratories to assess the quality of analytical processes. A parameter associated to a Sigma >6 is considered "world class" whereas a Sigma <3 is "poor" or "unacceptable". The aim of this retrospective study was to quantify the impact of different approaches for Sigma metrics calculation. Read More

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Health information exchange in relation to point-of-care testing in home care: Issues in Japan.

Clin Chim Acta 2022 May 17. Epub 2022 May 17.

Division of Community and Family Medicine, Center for Community Medicine, Jichi Medical University, Shimotsuke-City, Japan; Department of Clinical Laboratory Medicine, Faculty of Medicine, Jichi Medical University, Shimotsuke-City, Japan. Electronic address:

Background: Laboratory tests, especially point-of-care testing (POCT), and related health information exchange (HIE) are necessary for patient management in the home care setting, where clinic-hospital cooperation and interprofessional collaboration are important.

Methods: We raised the issues ahead of HIE in relation to POCT in home care in Japan, including issues in electronic medical record use, localized interprofessional collaboration networks with information and communication technology, personal health record use and open connectivity.

Results: HIE system may depend on the initiatives of expert communities with non-expert partnership, as well as national healthcare policies. Read More

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Theoretical and experimental study of the infrared and Raman spectra of L-lysine acetylation.

Spectrochim Acta A Mol Biomol Spectrosc 2022 May 12;278:121371. Epub 2022 May 12.

CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institute of Intelligent Agriculture Institute of Intelligent Machines, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China. Electronic address:

Acetylation is a common and extremely important protein modification in biology, referring to the covalent attachment of an acetyl group to the amino group. There are two forms of protein acetylation, which are lysine Nε-acetylation and N-terminal Nα-acetylation, respectively. Protein lysine Nε-acetylation is a globally important post-translational modification which plays a critical regulatory role in almost all aspects of cell metabolism. Read More

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Molecular Epidemiology, Species Distribution, and Zoonotic Importance of the Neglected Meat-Borne Pathogen Sarcocystis spp. in Cattle (Bos taurus): A Global Systematic Review and Meta-analysis.

Acta Parasitol 2022 May 20. Epub 2022 May 20.

Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization, Mashhad, Iran.

Background: Sarcocystis species are diverse apicomplexan parasites, though only two zoonotic species (S. hominis and S. heydorni) circulate between cattle and humans. Read More

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Taxonomy and Systematics of Two New Species of Myxobolus (Cnidaria: Myxobolidae) Parasitizing the Gills of Mugil curema (Mugilidae) from the Brazilian Coast.

Acta Parasitol 2022 May 20. Epub 2022 May 20.

Análise de Sistemas Ambientais, Centro Universitário CESMAC, Maceió, AL, Brazil.

Purpose: We describe two new species of Myxobolus (Myxobolidae) found parasitizing Mugil curema from two locations in Brazil: Myxobolus curemae n. sp. from gill arch and rays and Myxobolus maceioensis n. Read More

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Complications and cosmetic outcomes of materials used in cranioplasty following decompressive craniectomy-a systematic review, pairwise meta-analysis, and network meta-analysis.

Acta Neurochir (Wien) 2022 May 20. Epub 2022 May 20.

Computational Neurosciences Outcomes Center, Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, USA.

Background: Optimal reconstruction materials for cranioplasty following decompressive craniectomy (DC) remain unclear. This systematic review, pairwise meta-analysis, and network meta-analysis compares cosmetic outcomes and complications of autologous bone grafts and alloplasts used for cranioplasty following DC.

Method: PubMed, Embase, and Cochrane were searched from inception until April 2021. Read More

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Correction to: Ultrastructural and biochemical classification of pathogenic tau, α-synuclein and TDP-43.

Acta Neuropathol 2022 May 20. Epub 2022 May 20.

Department of Brain and Neuroscience, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, 156-8506, Japan.

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European Portuguese Language and Cultural Validation of the Chronic Obstructive Pulmonary Disease Assessment Test.

Acta Med Port 2022 May 20. Epub 2022 May 20.

Faculty of Medicine. University of Coimbra. Coimbra. Coimbra Institute for Clinical and Biomedical Research (iCBR). Faculty of Medicine. University of Coimbra. Coimbra. Clinical Academic Center of Coimbra (CACC). Coimbra. Portugal.

Introduction: The chronic obstructive pulmonary disease assessment test (CAT) is a self-administered questionnaire that measures health-related quality of life. GOLD proposes using this questionnaire, since it provides thorough coverage of the impact of chronic obstructive pulmonary disease (COPD) on well-being. This questionnaire has been widely used in daily clinical practice in Portugal, but it lacks validation for European Portuguese. Read More

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DDR1 promotes LoVo cell proliferation by regulating energy metabolism.

Acta Biochim Biophys Sin (Shanghai) 2022 Mar 25. Epub 2022 Mar 25.

Department of Gastroenterology, The Second Hospital of Lanzhou University, Lanzhou 730030, China.

Cellular energy metabolism dysregulation is associated with colorectal cancer (CRC) development and progression. Discoidin domain receptor1a (DDR1a), one of the five DDR1 isoforms, is closely related to cell proliferation, invasion, and apoptosis in various tumors. Whether it participates in cellular metabolic reprogramming and regulates CRC initiation and progression remains unclear. Read More

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