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Acta Histochem 2020 Jul 20;122(5):151575. Epub 2020 Jun 20.

Laboratory of Morphological and Immunohistochemical Studies, Department of Physical Education, São Judas Tadeu University, São Paulo, SP, Brazil; Dante Pazzanese Institute of Cardiology, São Paulo, SP, Brazil.

Menopause is a major factor involved in dyslipidemia increasing the risk of atherosclerosis which may be reversed by a routine of aerobic physical activity. Thus, this study aimed to analyze the effects of aerobic training on the thoracic aorta of female LDL-receptor knockout mice submitted to estrogen deprivation. Fifteen genetically modified female mice, knockout for the low-density lipoprotein receptor (LDL-Knockout group) were used as experimental groups and fifteen wild female mice (C57BL/6 J) were used as control groups. Read More

Acta Histochem 2020 Jul 28;122(5):151570. Epub 2020 May 28.

Laboratory of Morphological and Immunohistochemical Studies, Department of Physical Education, São Judas Tadeu University, São Paulo, SP, Brazil.

Sarcopenia is a common condition that is associated mainly with hormonal factors, nutritional status, physical activity, leading to a lower quality of life. Thus, this study aimed to evaluate the effects of diets with vegetable or animal proteins (AP) associated with resistance training on the structure of the soleus muscle in aged Wistar rats. The histochemical technique was used for the typing of muscle fibers, the cross-sectional area of myocytes, and volume densities of myocytes and interstitium. Read More

Acta Histochem 2020 Jul 6;122(5):151557. Epub 2020 Jun 6.

School of Pharmacy, Lanzhou University, 730000, Lanzhou, Gansu, China.

Lappaconitine sulfate (LS) has good solubility and bioavailability. We have previously studied the anti-proliferative activity of LS on colon cancer HT-29 cell, but its anti-proliferative activity and molecular mechanism on human non-small cell lung cancer A549 cells are still unclear. This study was to investigate the effects of LS on proliferation, cell cycle and apoptosis in human non-small cell lung cancer A549 cells, and its possible molecular mechanisms. Read More

Acta Histochem 2020 Jul 20;122(5):151548. Epub 2020 May 20.

Cesare Maltoni Cancer Research Center, Ramazzini Institute, via Saliceto 3, 40010 Bentivoglio, Bologna, Italy. Electronic address:

Lymphomas and leukaemias involving the lung have in some cases been hard to distinguish from respiratory tract infection in Sprague-Dawley (SD) rats from long-term bioassays. In order to differentiate between tumours and immune cell infiltrates, updated pathological criteria and nomenclature were used and immunohistochemistry (IHC) was applied to haematopoietic and lymphoid tissue tumours (HLTs) in the original prenatal long-term Aspartame (APM) study performed by the Ramazzini Institute (RI). All 78 cases of HLTs from treated and control groups were re-examined based on light microscopic morphological characteristics and subjected to a panel of IHC markers including Ki67, CD3, PAX5, CD20, CD68, TdT, CD45, CD14 and CD33. Read More

Acta Histochem 2020 Jul 26;122(5):151568. Epub 2020 May 26.

Oral Biology Department, Faculty of Dentistry, Tanta University, Tanta, Egypt.

Structural changes in parotid gland (PG) were previously reported following ablation of thyroid gland. However, the functional alterations (especially for proteins) have not been elucidated yet. Herein, we investigated the effect of rat thyroidectomy on PG structure and protein content and studied the ability of thyroxin-supplementation to alleviate the associated structural and functional changes. Read More

Acta Histochem 2020 Jul 20;122(5):151554. Epub 2020 May 20.

Department of Medical Histology and Cell Biology, Faculty of Medicine, Mansoura University, Egypt. Electronic address:

Various studies reported the possibility of deterioration of blood-brain barrier (BBB) integrity owing to the aging process. The current work was performed to investigate the ability of Monosodium glutamate (MSG) to cross BBB in aged rats, the damage affecting the anterior horn cells of the spinal cord due to excitotoxicity, and the mechanisms by which quercetin (Que) administration might suppress such damage. Forty male rats aged 18 months were assigned equally to 4 groups: control group, Que group (received Que, 20 mg/kg/d intraperitonealy for 10 days), MSG group (received MSG, 4. Read More

Acta Histochem 2020 Jul 26;122(5):151559. Epub 2020 May 26.

Federal Rural University of Pernambuco, Department of Morphology and Animal Physiology, Recife CEP 52171-900, Brazil.

The aim of the present study was to analyze the effects of melatonin treatment on diabetic rat testes. Fifty albino rats were divided into the following groups: CG: control group; GD: placebo-induced and placebo-treated mice; GDI: insulin-induced and post-confirmation diabetes-induced rats; GDM: diabetes-induced and melatonin-treated post-confirmation mice and GDMS: diabetes-induced and melatonin-treated mice simultaneously. Melatonin was administered at a dose of 10 mg/kg in drinking water every day for 20 days at night. Read More

Acta Histochem 2020 Jul 15;122(5):151574. Epub 2020 Jun 15.

Dept. of Trauma Surgery, Medical Faculty, University of Magdeburg, 39120 Magdeburg, Germany.

Motor neuronal damage due to diseases, traumatic insults or de-afferentation of the spinal cord is often incurable because of poor intrinsic regenerative capacity. Hence, medical basic research has to provide a better understanding of development-/regeneration-related cellular processes as only way to develop new and successful therapeutic strategies. Here, we investigated the neuronal differentiation of the NSC-34 hybrid cell line, which is an accepted model for spinal cord motor neurons. Read More

Acta Histochem 2020 Jul 20;122(5):151552. Epub 2020 May 20.

İstanbul University, İstanbul Medicine Faculty, Histology and Embryology Department, Fatih, İstanbul, Turkey.

Healing of diabetic wounds are delayed due to late initiation and prolongation of the inflammatory phase, and inadequate growth factor synthesis, which may lead to chronic ulcers that may cause limb amputation, besides making the patients vulnerable to infections. In recent years, it has been extensively discussed whether different cell types transplanted to diabetic wound models accelerate wound healing. In this study, the effect of dermis-derived cells on Streptozotocin (STZ) induced experimental diabetic Sprague-Dawley rats were investigated. Read More

Acta Histochem 2020 Jul 16;122(5):151571. Epub 2020 Jun 16.

Department of Neurology, The First Affiliated Hospital of China Medical University, Shenyang 110001, People's Republic of China. Electronic address:

Emerging evidence has unraveled the important implications of microRNAs (miRNAs/miRs) in intracerebral hemorrhage (ICH). The aim of the present study was to assess the possible regulatory role of miR-26a-5p in ICH both in vivo and in vitro. ICH model of rats was constructed using stereotactic injection of VII collagenase, and ICH condition of PC-12 cells was stimulated by hemin. Read More

Acta Histochem 2020 Jul 20;122(5):151556. Epub 2020 May 20.

Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran; Endometrium and Endometriosis Research Centre, Hamadan University of Medical Sciences, Hamadan, Iran. Electronic address:

Asherman's syndrome (AS) is an endometrial damage that results in infertility in women. Although stem cell therapy has been introduced as a potential treatment for this syndrome, its use in clinical settings remains challenging because of the likelihood of contamination and cell differentiation. Herein, we investigated the effects of adipose-derived stromal vascular fraction (SVF) transplantation on proliferation and angiogenesis in the endometrium in an AS model. Read More

Acta Histochem 2020 Jul 13;122(5):151572. Epub 2020 Jun 13.

Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address:

Application of a three-dimensional (3D) culture system for in vitro proliferation and differentiation of human spermatogonial stem cells (SSCs) is a useful tool for the investigation of the spermatogenesis process and the management of male infertility particularly in prepubertal cancer patients. The main purpose of this study was to investigate the proliferation of human SSCs co-cultured with Sertoli cells in soft agar culture system (SACS) supplemented by Laminin and growth factors. Testicular cells were isolated from testes of brain-dead patients and cultured in two-dimensional (2D) culture system for 3 weeks. Read More

Acta Histochem 2020 Jul 20;122(5):151558. Epub 2020 May 20.

Laboratory of Neurobiology, Faculty of Veterinary Medicine and Animal Science, Poznan University of Life Sciences, Wojska Polskiego 71C, 60-625 Poznan, Poland. Electronic address:

Obesity, which reaches an epidemic, is characterized by alterations in metabolic and hormonal profiles. Moreover, uncontrolled obesity may lead to development of diabetes type 2, which accounts for about 90% of all diabetic cases. In obesity, besides changes in metabolism, numerous co-morbidities are reported, e. Read More

Acta Histochem 2020 Jul 10;122(5):151569. Epub 2020 Jun 10.

Division of Microscopic and Developmental Anatomy, Department of Anatomy, Kurume University School of Medicine, Kurume, Fukuoka, Japan.

Purpose: The fate of subcutaneously transplanted urogenital sinus (UGS) and seminal vesicle (SV) was investigated in the present study.

Materials And Methods: Fetal UGS and SV extracted from 20-embryonic-day-old male normal and GFP transgenic rats were subcutaneously transplanted into 7-week-old male immunologically inhibited rats. The transplants were then examined at 2, 4, 8, and 16 weeks after transplantation. Read More

Acta Histochem 2020 Jul 13;122(5):151573. Epub 2020 Jun 13.

Laboratory of Physiology, A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok 690041, Russia. Electronic address:

Stroke-induced changes in neuroglia determine the basic conditions for the survival and damage of neurons in the ischemic core. Here, we studied the immunolocalization of glial cell line-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), ionized calcium-binding adaptor molecule 1 (Iba-1), and S-100β in the rat parietal cortex after constant occlusion of the middle cerebral artery. These cytoplasmic proteins are specific for different glial cell types. Read More

Acta Histochem 2020 Jul 4;122(5):151553. Epub 2020 May 4.

Department of Physiology and Pathophysiology, The Fourth Military Medical University, Xi'an, 710032, China; Department of Anesthesiology, Ankang Central Hospital, Ankang, 725000, China. Electronic address:

Neuroinflammation plays a key role in perioperative neurocognitive disorders (PND). Increased evidences indicate that triggering receptor expressed on myeloid cells 2 (TREM2) can mitigate inflammatory response in the brain, and the aim of this study is to investigate whether TREM2 is involved in surgery-induced cognitive dysfunction in adult mice. We used adult C57BL/6 mice subjected to intramedullary fixation surgery, and found that surgery did not impair the motor ability of mice, but worsened the learning and memory function, and reduced the expression of TREM2. Read More

Acta Histochem 2020 May 4;122(4):151549. Epub 2020 May 4.

Department of Orthopedics, The Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha 410005, Hunan Province, PR China. Electronic address:

Background: Astragalus saponin IV(AS- IV) extracted from tranditional Chinese medicine Radix Astragali Mongolici, which had been reported to have medicinal properties in treating several types of diseases. This study aimed at investigating the biological functions of AS-IV on bone marrow mesenchymal cells(BMSCs) differentiation, therefore, seeking for a better application of AS-IV on fracture or other orthopedic disorders.

Methods: AS-IV was co-incubated with BMSCs in vitro to testify whether it can influence the proliferation and differentiation of BMSCs. Read More

Acta Histochem 2020 May 25;122(4):151551. Epub 2020 Apr 25.

Department of Microbiology, Harbin Medical University, Harbin, Heilongjiang, 150086, People's Republic of China. Electronic address:

Vascular calcification (VC) is a vital factor for cardiovascular morbidity and mortality. Accumulating data suggest that microRNA (miR) is implicated in the VC. The main purpose of this study is to study the influence of miR-155-5p overexpression on VC development in vitro and in vivo. Read More

Acta Histochem 2020 May 21;122(4):151539. Epub 2020 Apr 21.

College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China. Electronic address:

The natural coat color is an important trait of vertebrate animals. For example, the coat color can help avoid harm to human beings caused by chemical dyeing, and it has economic significance for domestic animals. The bone morphogenetic protein 4 (BMP4) and its antagonist Noggin can regulate pigmentation and the generation of coat color in mice; thus, they may also regulate the coat color of Kazakh sheep. Read More

Acta Histochem 2020 May 14;122(4):151550. Epub 2020 Apr 14.

Shanxi Key Laboratory of Stem Cells for Immunological Dermatosis, Institute of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, No. 5 Dong San Dao Xiang, Jiefang Road, Taiyuan, 030009, Shanxi Province, China. Electronic address:

Background: Psoriasis is a chronic recurrent inflammatory disease. Mesenchymal stem cells (MSCs) can regulate the inflammatory microenvironment, thereby controlling the proliferation, differentiation, and migration of immune cells. Connexin 43(Cx43), a key gap junction protein, has been shown to form gap junctions for communication between neighboring cells. Read More

Acta Histochem 2020 May 10;122(4):151540. Epub 2020 Apr 10.

School of Dentistry, University of Liverpool, Liverpool, United Kingdom.

The presence of organic secretion in ductal cells of the sublingual salivary gland of ferret has been questioned, which prompted the present investigation. Paraffin or cryostat sections from aldehyde fixed or quenched sublingual glands of this species were tested for some amino acid residues, mucosubstances, oxidative and hydrolytic enzymes, and lectins. The glands showed inconspicuous ducts of simple appearances on routine histology. Read More

Acta Histochem 2020 May 18;122(4):151537. Epub 2020 Mar 18.

Department of Hand, Plastic and Aesthetic Surgery, University of Munich, Pettenkoferstr. 8a, 80336, Munich, Germany.

The understanding of fat tissue plays an eminent role in plastic surgery as well as in metabolic research. Histopathological analysis of tissue samples provides insight in free fat graft survival and culture experiments help to better understand fat tissue derived stem cells (ASCs). To facilitate such experiments, modern image-based histology could provide an automatized approach to a large amount of data to gain not only qualitative but also quantitative data. Read More

Acta Histochem 2020 Apr 17;122(3):151533. Epub 2020 Mar 17.

Department of Medical Histology and Cell Biology, Faculty of Medicine, Mansoura University, Egypt.

Liraglutide is a new therapy used in diabetes and its effect on diabetic complications particularly cardiovascular ones is still under investigated. In our research, we tried to study the effect of liraglutide on experimental diabetic cardiomyopathy (DCM) induced by streptozotocin. We found that liraglutide nearly preserved normal myocardiac structure and significantly protected against myocardiac inflammation and fibrosis that was found in DCM group, p < 0. Read More

Acta Histochem 2020 May 14;122(4):151538. Epub 2020 Mar 14.

Department of Gastroenterology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, People's Republic of China.

Background: Esophageal cancer is a digestive tract malignancy, ranking sixth among the world's deadliest tumor incidence. However, the pathogenesis of esophageal cancer is complex and the prognosis remains poor. Therefore, in-depth study of the pathogenesis and developing effective treatments are of great value for esophageal cancer. Read More

Acta Histochem 2020 May 8;122(4):151536. Epub 2020 Mar 8.

Division of Anatomy, Faculty of Dental Medicine, "Carol Davila" University of Medicine and Pharmacy, 8 Eroilor Sanitari Blvd., RO-050474, Bucharest, Romania. Electronic address:

The lacrimal sac (LS) empties in the nasolacrimal duct to drain the tears in the inferior nasal meatus. Different studies indicated the role of the lacrimal pump in the lacrimal drainage. Although controversial, the lacrimal pump mechanism is an extrinsic one, either active, or passive. Read More

Acta Histochem 2020 Apr 8;122(3):151528. Epub 2020 Mar 8.

Department of Orthopaedics, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, 519000, Guangdong, China.

Backgroud: Osteoarthritis (OA) is a common disease caused by chondrocyte dysfunction. KLF10 is a member of the Sp1-like transcription factor family that is involved in regulating osteoblasts, but its expression and regulatory mechanism(s) in chondrocytes are unclear. In the present study, we aimed to investigate the regulatory role of KLF10 on the pathological process of OA. Read More

Acta Histochem 2020 May 6;122(4):151534. Epub 2020 Mar 6.

Anatomy and Embryology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt. Electronic address:

Objective: Although cisplatin (CIS) acts as potent chemotherapy, nephrotoxicity still its major life-threatening side effect. The purpose of this study was to discuss and compare the renoprotective effects of curcumin (CUR) and etoricoxib (ETB) against CIS-induced nephrotoxicity.

Materials & Methods: Thirty six adult female rats were divided equally into 6 groups: Group I (control), Group II (CIS) received cisplatin (7. Read More

Acta Histochem 2020 Apr 3;122(3):151532. Epub 2020 Mar 3.

Department of Biosciences and Territory, University of Molise, Contrada Fonte Lappone, 86090 Pesche (Isernia), Italy. Electronic address:

Aberrant content of sialic acids (Sias) has been observed in various human cancer types in different organs. Sias have been implicated in cancerous transformation, invasiveness and metastasis, and in the escaping of cancer cells from immune surveillance. Indeed, Sias are commonly regarded as important biomarkers to distinguish cancer cells from their healthy counterparts. Read More

Acta Histochem 2020 Apr 3;122(3):151529. Epub 2020 Mar 3.

Department of Agronomy-Entomology, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros s / n, Dois Irmãos, 52171-900, Recife, PE, Brazil. Electronic address:

Agricultural pest control is a popular research topic, and essential oils are widely studied because they represent a promising alternative to synthetic insecticides. However, despite the increase in studies on pests, little work has been done on pesticide contamination of the predators feeding on insecticide-affected prey. Therefore, the objective of this study was to evaluate the effects of Lethal Dose 50 (LD) from the essential oils of Mentha spicata L. Read More

Acta Histochem 2020 Apr 2;122(3):151531. Epub 2020 Mar 2.

Tallinn University Centre of Excellence in Neural and Behavioural Sciences, School of Natural Sciences and Health, Tallinn University, Tallinn, Estonia; Division of Neuropsychopharmacology, Department of Psychology, University of Tartu, Tartu, Estonia. Electronic address:

Ketamine is a noncompetitive antagonist of glutamatergic N-methyl-d-aspartate receptors. Its acute effects on healthy volunteers and schizophrenia patients mimic some acute psychotic, but also cognitive and negative symptoms of schizophrenia, and subchronic treatment with ketamine has been used as an animal model of psychotic disorders. Glutamatergic neurotransmission is tightly coupled to oxidative metabolism in the brain. Read More

Acta Histochem 2020 Apr 27;122(3):151530. Epub 2020 Feb 27.

Department of Experimental and Clinical Medicine, Section of Anatomy and Histology, University of Florence, Largo Brambilla 3, 50134, Florence, Italy. Electronic address:

Telocytes (TCs) have recently emerged as a peculiar type of stromal cells located in both perivascular and interstitial compartments of multiple anatomical sites in humans, other mammals and vertebrates. Pioneer electron microscopy studies have ultrastructurally defined TCs as "stromal cells with telopodes" (i.e. Read More

Acta Histochem 2020 Apr 26;122(3):151527. Epub 2020 Feb 26.

Department of Obstetrics and Gynecology, The First Hospital of Jilin University, Changchun 130021, People's Republic of China. Electronic address:

MicroRNA-214-5p has been reported to be expressed in placental tissue and suppressed the proliferation and invasion of various tumor cells. However, the role of miR-214-5p in pre-eclampsia has not been reported. We aimed to explore the effects of miR-214-5p in proliferation, migration, and invasion of placental trophoblast cells. Read More

Acta Histochem 2020 Apr 22;122(3):151526. Epub 2020 Feb 22.

University Centre of Veterinary Medicine, University of Agriculture in Krakow, Mickiewicza 24/28, 30-059, Krakow, Poland.

Communication in biological systems involves diverse-types of cell-cell interaction including cross-talk between receptors expressed by the target cells. Recently, novel sort of estrogen receptors (G protein - coupled estrogen receptor; GPER and estrogen-related receptor; ERR) that signal directly via estrogen binding and/or via mutual interaction-regulated estrogen signaling were reported in various organs including testis. Peroxisome proliferator - activated receptor (PPAR) is responsible for maintaining of lipid homeostasis that is critical for sex steroid production in the testis. Read More

Acta Histochem 2020 Apr 18;122(3):151515. Epub 2020 Feb 18.

Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-11 Inada-cho, Obihiro, Hokkaido, 080-8555, Japan; Laboratory of Sustainable Animal Environment, Graduate School of Agricultural Science, Tohoku University, 232-3 Yomogida, Naruko-onsen, Osaki, Miyagi, 989-6711, Japan.

Most mammalian species have a vomeronasal organ that detects specific chemical substances, such as pheromones. Mucous fluid covering the vomeronasal sensory epithelium is secreted by vomeronasal glands, and the properties of these fluids have been suggested to be involved in chemical detection. Histological studies using periodic acid-Schiff (PAS) and Alcian blue pH 2. Read More

Acta Histochem 2020 Apr 12;122(3):151513. Epub 2020 Feb 12.

Electron Microscopy Unit, Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.

Arf6 (ADP ribosylation factor 6), activated by Arf-GEF (guanine nucleoside exchange factor), is involved in the membrane trafficking and actin-remodeling which are critical for maintenance of cell organization and activity and for fusion of myoblasts to form myotubes/myofibers. EFA6A (exchange factor for Arf6 type A) and BRAG2 (brefeldin A-resistant Arf-GEF 2) represent members of discrete subfamilies of Arf-GEF, while PIP5Kγ (phosphatidylinositol4-phosphate5-kinase γ) produces PI 4,5-bisphosphate (PIP2) and it is target for Arf6. In the present study, immunoreactive bands for Arf6, EFA6A, BRAG2 and PIP5Kγ were detected in immunoblots of skeletal muscle homogenates of mice at E18D (embryonic day 18), while the bands for Arf6, EFA6A and PIP5Kγ were reduced in density and no significant bands for BRAG2 were discerned at P1D (postnatal 1 day). Read More

Acta Histochem 2020 Apr 3;122(3):151510. Epub 2020 Feb 3.

Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Histology and Embryology Department, Turkey. Electronic address:

Calcitonin is expressed in the epithelium of endometrium, and modulates zonula adherens junctions which are composed of cadherin-catenins complex during the implantation window. Trophoblastic cells which have complex interaction with the epithelial cells of endometrium during implantation were demonstrated to have calcitonin receptors. Mechanism of action of calcitonin on trophoblastic cells has not yet been elucidated. Read More

Acta Histochem 2020 Apr 1;122(3):151514. Epub 2020 Feb 1.

Department of Neurosurgery, Tong Ji Hospital, Tong Ji University School of Medicine, Shanghai, 200065, China. Electronic address:

To explore the potential effects of andrographolide on chronic cerebral hypoperfusion (CCH)-induced neuronal damage as well as the underlying mechanisms. Rat CCH model was established by 2-vessel occlusion (2VO). The CCH rats received andrographolide treatment for 4 weeks. Read More

Acta Histochem 2020 Feb 31;122(2):151512. Epub 2020 Jan 31.

California State University, Northridge, CA, USA. Electronic address:

Acta Histochem 2020 Apr 31;122(3):151506. Epub 2020 Jan 31.

Institute of Basic Medical Sciences, Center for Diabetic Systems Medicine (Guangxi Key Laboratory of Excellence), Guilin Medical University, Guangxi, Guilin, 541100, China; Hunan Province Innovative Training Base for Medical Postgraduates, University of South China and Yueyang Women & Children's Medical Center, Institute of Reproductive Medicine, Yueyang, Hunan, 416000, China. Electronic address:

Circular RNA (CircRNA), a type of endogenous non-coding RNAs (ncRNAs), is generally generated from precursor mRNA (pre-mRNA) by canonical splicing and head-to-tail back splicing. The structure without a polyA tail renders circRNA highly insensitive to ribonuclease. Simultaneously, the distribution of circRNAs is tissue and developmental stage-specific. Read More

Acta Histochem 2020 Apr 25;122(3):151511. Epub 2020 Jan 25.

Department of Rehabilitation Sciences, Kobe University Graduate School of Health Sciences, Kobe, Japan. Electronic address:

Migration of the macrophages to the injured site soon after the skeletal muscle injury is crucial for subsequent regeneration of the muscle fibers. The Monocyte chemoattractant protein-1 (MCP-1) is important chemokine for regulating migration of the monocytes/macrophages. Earlier reports have discussed that icing applied soon after muscle crush injury retards muscle regeneration through retardation of macrophage migration. Read More

Acta Histochem 2020 Apr 21;122(3):151508. Epub 2020 Jan 21.

College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, China. Electronic address:

Melanoma is a highly invasive malignant skin tumor having high metastatic rate and poor prognosis. The biology of melanoma is controled by miRNAs. The miRNA-183 cluster, which is composed of miRNA-183∼96∼182 genes, plays an important roles in tumor development. Read More

Acta Histochem 2020 Apr 18;122(3):151509. Epub 2020 Jan 18.

College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, PR China. Electronic address:

It was well known that a critical process of oogenesis in the female mammalian was the entry of mitotic oogonia into meiosis. Early studies from model animal mice suggested that the retinoic acid (RA) response signal protein STRA8 (stimulated by retinoic acid gene 8) and the meiosis-specific chromosomal behavior marker protein SCP3 (Synaptonemal Complex Protein 3) were two crucial molecular markers during meiosis. The expression of STRA8 and SCP3 at different stages in rat ovaries was investigated by immunohistochemistry, qRT-PCR and Western Blot. Read More

Acta Histochem 2020 Apr 17;122(3):151505. Epub 2020 Jan 17.

Pathology Department, Farhet Hached University Hospital, Sousse, Tunisia.

The utility of the expression lack of DNA mismatch-repair (MMR) proteins in the detection of Lynch syndrome in endometrial hyperplasia as precursor lesion of endometrial carcinoma has not been well-established. The study investigated the immunoexpression pattern of MMR proteins in endometrial hyperplasia from Tunisian patients. We carried out a retrospective study of 60 endometrial hyperplasias diagnosed among Tunisian patients. Read More

Acta Histochem 2020 Apr 16;122(3):151507. Epub 2020 Jan 16.

Laboratory of Veterinary Anatomy and Cell Biology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan. Electronic address:

Vesicular monoamine transporters (VMAT) 1 and 2 are responsible for monoamine transportation into secretary vesicles and are tissue-specifically expressed in central and peripheral monoaminergic tissues, including the carotid body (CB). The aim of the present study was to examine the expression of catecholamine-synthesizing enzymes in VMAT1- and VMAT2-immunoreactive glomus cells in the rat CB using multiple immunolabeling. The expression of VMAT1 and VMAT2 mRNA in the CB was confirmed by RT-PCR. Read More

Acta Histochem 2020 Feb 17;122(2):151504. Epub 2020 Jan 17.

Laboratory of Morphometry, Metabolism, and Cardiovascular Diseases, Biomedical Center, Institute of Biology, The University of the State of Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address:

Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have benefits in the metabolism of adipose tissue. However, its contribution to the adipogenesis is not entirely elucidated. The study aimed to evaluate the effects of EPA and DHA on adipogenesis, especially in the PPARγ (peroxisome proliferator-activated receptor-gamma) and Cidec (cell death-inducing DFFA-like effector c) pathway. Read More

Acta Histochem 2020 Feb 16;122(2):151503. Epub 2020 Jan 16.

Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Alexandria University, Egypt.

The current study aimed to investigate the tongue (lingual) morphometry, histology, and histochemistry of two chiropterans endemic in the Egyptian fauna, and having different feeding preferences. The tongues of nine adult individuals of each species were utilized in our investigation. The tongue of fruit-eating bat was observed relatively longer than the one of insect-eating bat. Read More

Acta Histochem 2020 Feb 10;122(2):151502. Epub 2020 Jan 10.

Laboratory for Neurocardiology, Department of Anatomy, Histology and Embriology, University of Split School of Medicine, Šoltanska 2, 21000, Split, Croatia; Department of Nuclear Medicine, Clinical Hospital Centre Split, Spinčićeva 1, Split, Croatia.

Vitamin D is a steroid hormone with numerous actions in the organism. There are strong evidences that relate vitamin D deficiency with liver lipid metabolism disturbances, but the mechanism of this action is still unknown. In our previous work we postulated the localization and accumulation of vitamin D receptor (VDR) in membrane of the lipid droplets (LDs) in hepatocytes. Read More

Acta Histochem 2020 Feb 7;122(2):151500. Epub 2020 Jan 7.

Institute of Neurobiology, Bulgarian Academy of Sciences, Sofia, Bulgaria; Department of Anatomy and Histology, Medical University of Sofia, Sofia, Bulgaria. Electronic address:

The carotid body (CB) is a multipurpose metabolic sensor that acts to initiate cardiorespiratory reflex adjustments to maintain homeostasis of blood-borne chemicals. Emerging evidence suggests that nitric oxide increases the CB chemosensory activity and this enhanced peripheral chemoreflex sensitivity contributes to sympathoexcitation and consequent pathology. The aim of this study was to examine by means of NADPH-diaphorase histochemistry and nitric oxide synthase (NOS) immunohistochemistry the presence and distribution of nitrergic structures in the CB of spontaneously hypertensive rats (SHRs) and to compare their expression patterns to that of age-matched normotensive Wistar rats (NWRs). Read More

Acta Histochem 2020 Feb 2;122(2):151484. Epub 2020 Jan 2.

Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil. Electronic address:

The aim of this study was to evaluate whether the addition of synthetic polymers to the vitrification solution affected follicular morphology and development and the expression of Ki-67, Aquaporin 3 (AQP3) and cleaved Caspase-3 proteins in ovarian tissue of the caprine species. Caprine ovaries were fragmented and two fragments were immediately fixed (Fresh Control) for morphological evaluation, while other two were in vitro cultured for 7 days (Cultured Control) and fixed as well. The remaining fragments were distributed in two different vitrification groups: Vitrified and Vitrified/Cultured. Read More

Acta Histochem 2020 Feb 30;122(2):151497. Epub 2019 Dec 30.

Electron Microscopy Unit, Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. Electronic address:

Our previous study demonstrated that, different from the parotid and sublingual glands, the submandibular glands of adult mice did not show an immunoblot band for PLCβ3 which is critical in the secretion mechanism by muscarinic cholinergic signaling. Therefore, the submandibular glands of mice at various stages of postnatal development were examined for this enzyme molecule in immunoblot and immunohistochemistry. In immunoblot, a weak band for PLCβ3-expression was detected only at early postnatal stages. Read More