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Acta Histochem 2019 Apr 9. Epub 2019 Apr 9.

Section of Zoology, Department of Biology, Faculty of Science, Ege University, İzmir, Turkey.

The fat body, originates from mesoderm, has many metabolic functions which changes as the embryonic development of the insect progresses. It plays an important role in the intermediate metabolism and in the metabolism of proteins, lipids and carbohydrates. It has roles in synthesis, absorption and storage of nutrients from hemolymph. Read More

Acta Histochem 2019 Apr 8. Epub 2019 Apr 8.

Department of Medical Histology & Cell Biology, Mansoura Faculty of Medicine, Egypt; Department of Histology, Hours University, Egypt. Electronic address:

Introduction: Pesticides are responsible for many occupational health hazards among farmers in developing countries. Chlorpyrifos (CPF) is one of the broad-spectrum organophosphorus (OP) insecticides used for agricultural, domestic and industrial purposes.

Aim Of The Work: The present study was designed to examine the effects of CPF on cardiac muscles and to evaluate the possible protective role of crocin using biochemical and histological methods with the intention to recognize the molecular tools of its probable cardioprotective effects. Read More

Acta Histochem 2019 Apr 3. Epub 2019 Apr 3.

Children's Medical Center, The Second Hospital of Shandong University, Jinan, Shandong, 250033, People's Republic of China. Electronic address:

β-Hydroxybutyric acid (BHBA) reportedly has neuroprotective and anti-oxidation properties. The present study aimed to investigate the protective effects of BHBA against epilepsy. C57BL/6 J mice were exposed to lithium chloride and pilocarpine to induce epilepsy and then were administrated with 300 mg/kg/day BHBA for 30 days. Read More

Acta Histochem 2019 Apr 3. Epub 2019 Apr 3.

Department of Anatomy, Cell Biology, Physiology and Biophysics, Institute of Biology, State University of Campinas (UNICAMP), Campinas SP, Brazil. Electronic address:

New tools are desirable to examine the metabolic state of individual cells within tissues. We proposed a fluorescence-based procedure consisting of acridine orange staining and fast green counterstaining (AO-FG) to improve the selectivity of the former for nucleic acids (acridine orange stains both DNA and RNA with different fluorescence colors), with no interference from proteins. We compared this test with the biochemical quantification of the relative amounts of RNA and DNA in selected rat ventral prostate samples and PC3 cells. Read More

Acta Histochem 2019 Apr 2. Epub 2019 Apr 2.

Department of Zoology, Modern College of Arts, Science and Commerce, Ganeshkhind, Pune, 411 016, Maharashtra, India.

Lepidophagy is comparatively rare amongst teleost fishes, yet our understanding of this specialization is lacking. Therefore we examined the digestive tract features of Pachypterus khavalchor using morphological, osteological, histological and histochemical techniques to comprehend and relate structural organization of digestive tract with scale eating habit. Morphologically, the alimentary canal is defined by a short and muscular esophagus, well-developed stomach and comparatively short intestine. Read More

Acta Histochem 2019 Apr 1. Epub 2019 Apr 1.

Ondokuz Mayis University Faculty of Arts and Sciences, TR-55139 Samsun, Turkey. Electronic address:

The anti-epileptic drugs phenobarbital and valproic acid have an extremely strong negative effect on cognitive processes such as learning and memory in the developing brain. We examined whether or not curcumin has protective effects on neuronal injury caused by these drugs in the developing rat brain. Young male Wistar rats were studied in two groups, a 7 days old and a 14 days old group (35 rats in each). Read More

Acta Histochem 2019 Mar 20. Epub 2019 Mar 20.

Department of Biomedical Sciences, College of Veterinary Medicine, Sudan University of Science and Technology, Khartoum, Sudan.

Basement membranes consist of various proteins, the major ones being laminin and collagen type IV. Primary defects in these two proteins have been extensively associated with kidney pathologies. This study aimed to establish baseline information on the immunohistochemical distribution of laminin and collagen type IV, and to corelate these with the ultrastructure of basal laminae in the uriniferous tubules of the dromedary camel. Read More

Acta Histochem 2019 Mar 16. Epub 2019 Mar 16.

Postgraduate Program in Veterinary Medicine (Clinics and Animal Reproduction), Faculty of Veterinary Medicine. Universidade Federal Fluminense, Niteroi, RJ, Brazil; Department of Veterinary Pathology and Clinic. Faculty of Veterinary Medicine. Universidade Federal Fluminense, Niteroi, RJ, Brazil. Electronic address:

The aim of this study was to evaluate the immunoexpression of HER-2 in gastric cells of cats infected with Non H. pylori Helicobacter (NHPH) and to investigate an association with the presence of inflammatory infiltrate. Forty-eight paraffin-embedded gastric samples were retrieved from the archives of the Veterinary Anatomic Pathology Laboratory that had previously been shown to be positive for NHPH with the rapid urease test and cytology. Read More

Acta Histochem 2019 Mar 15. Epub 2019 Mar 15.

College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China. Electronic address:

Angiotensin II (AngII) regulates pigment synthesis by tyrosinase in melanocytes. To evaluate the association between AngII and coat color formation, we detected the expression distribution of AngII in white and black sheep skins by LC-ESI-MS/MS, western blot, quantitative real-time-PCR (qPCR) and distribution of AngII by immunohistochemistry.Liquid chromatography-electrospray ionization tandem MS (LC-ESI-MS/MS) results showed that AngII was found in white and black skin tissues of sheep. Read More

Acta Histochem 2019 Mar 6. Epub 2019 Mar 6.

Faculty of Dentistry, Thammasat University, Rangsit campus, 99 Moo 18 Pahonyothin Rd., Klong Luang, Pathumthani, 12120, Thailand.

Introduction: Long-term administration of bisphosphonates (BPs) may cause osteonecrosis of the jaw (BRONJ). After administration, 50% of BPs in the circulation rapidly binds to calcium phosphate of bone. Two forms, bound and free BPs, may affect cells residing in bone including macrophages. Read More

Acta Histochem 2019 Mar 5. Epub 2019 Mar 5.

Department of Basic Medical Sciences, Faculty of Medicine, University of La Laguna, Tenerife, Spain.

Numerous lymphatic anastomosing channels in the lymph nodes are the most demonstrative finding of the rare lesion termed "vascular transformation of lymph node sinuses" (VTS). The mechanism of lymphatic vessel formation in VTS has not been studied. Vessel intussusception contributes to vascular expansion, and intraluminal pillars/posts, interstitial tissue structures or larger pillars (ITSs) and folds are the hallmarks of this process in blood vessels. Read More

Acta Histochem 2019 Mar 4. Epub 2019 Mar 4.

Department of Anatomy, Histology and Embryology, Medical University of Sofia, Bulgaria.

The claustrum is a bilateral subcortical nucleus situated between the insular cortex and the striatum in the brain of all mammals. It consists of two embryologically distinct subdivisions - dorsal and ventral claustrum. The claustrum has high connectivity with various areas of the cortex, subcortical and allocortical structures. Read More

Acta Histochem 2019 Apr 2;121(3):361-367. Epub 2019 Mar 2.

Department of Histology and Embryology, Cerrahpasa Medical Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey.

The main purpose of this study is to establish an effective cryopreservation protocol for the umbilical cord tissue as a source of mesenchymal stem cells (MSCs). In this context, it was aimed to use a cryoprotectant that could be an alternative to dimethyl sulfoxide (DMSO) which is commonly used despite the toxic side effects. Therefore, two different cryopreservation solutions were prepared using 10% DMSO and 10% 1,2 propanediol (PrOH). Read More

Acta Histochem 2019 Apr 28;121(3):354-360. Epub 2019 Feb 28.

Universidad de Buenos Aires, Facultad de Cs Veterinarias, Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Argentina. Electronic address:

The spatial organization of cells during tissue differentiation is a crucial process in the morphogenesis of vertebrates. This process involves the movement, separation, and connection of cells. It is essential to elucidate the molecular mechanisms involved in these processes for the understanding of animal morphogenesis. Read More

Acta Histochem 2019 Apr 26;121(3):376-379. Epub 2019 Feb 26.

Department of Structural Biology, Molecular and Genetic, State University of Ponta Grossa, Avenida Carlos Cavalcanti, 4748 - Campus Uvaranas - Uvaranas, Ponta Grossa, PR, 84030-900, Brazil; University of Ponta Grossa, Avenida Carlos Cavalcanti 4748, Campus Uvaranas, Uvaranas, 84030-900, Ponta Grossa, PR, Brazil. Electronic address:

Vilification in the chick gut involves the formation of longitudinal ridges, establishment of their zigzag pattern and emergence of individual villi. Although the morphological changes during vilification are well known in the chick gut, the pattern of cell proliferation during this process is poorly understood. The aim of the present study was to correlate spatial and temporal changes in cell proliferation to folding of the longitudinal ridges into zigzags. Read More

Acta Histochem 2019 Apr 23;121(3):330-343. Epub 2019 Feb 23.

Stem Cell Research and Application Unit, Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, 108, C.R Avenue, Kolkata, 700073, West Bengal, India. Electronic address:

Myelodysplastic syndrome is considered globally as heterogenous group of neoplasm which often proclaims leukemic progression. The heterogeneity is reflected not only in clinical manifestations of the disease but also in salient causes of disease development. In spite of multiple therapeutic modalities, shortfall towards treatment of this disorder still persists. Read More

Acta Histochem 2019 Apr 23;121(3):344-353. Epub 2019 Feb 23.

Institute of Veterinary Anatomy-, Histology and -Embryology, University of Giessen, 35392, Giessen, Germany.

Cell-based therapies have become a promising approach to promote tissue regeneration and the treatment of musculoskeletal disorders. Bone regeneration maintains bone homeostasis, mechanical stability and physical performance. Mechanical stimulation showed to induce stem cell differentiation into the osteogenic fate. Read More

Acta Histochem 2019 Apr 20;121(3):380-381. Epub 2019 Feb 20.

Dermatology, Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Italy.

Acta Histochem 2019 Apr 16;121(3):323-329. Epub 2019 Feb 16.

College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, China. Electronic address:

Myostatin (MSTN) is a key negative regulator of muscle growth and development. Skeletal, cardiac, and smooth muscles were isolated from MSTN knockout (MSTN-∕-) and control mice to investigate the effect of knocking out MSTN on peroxisome proliferator-activated receptor 1 coactivator (PGC-1α)-III and fibronectin domain 5 (FNDC5) expression. Various molecular biology techniques were used to analyze the changes in PGC-1α-FNDC5 in different muscle types from MSTN-∕- mice. Read More

Acta Histochem 2019 Apr 14;121(3):368-375. Epub 2019 Feb 14.

Institute of Higher Nervous Activity and Neurophysiology RAS, Moscow, Butlerova, 5a, 117485, Russian Federation.

Lentiviral transduction is a powerful tool and widely used in neuroscience research to manipulate gene expression of cells. However, the injection of lentiviral vectors in the brain is not totally benign, it potentially induces focal neuroinflammation. Upon inflammation, microglial cells get activated and can induce major changes in tissue environment, which may interfere with experimental results. Read More

Acta Histochem 2019 Apr 10;121(3):311-322. Epub 2019 Feb 10.

Ankara University, Faculty of Veterinary Medicine, Department of Histology and Embryology, Ankara, Turkey.

Peyer's patches are known as the immune sensors of the intestine because of their ability to transport luminal antigens. The objective of this study was both to assess the prenatal and postnatal development of sheep ileal Peyer's patches with respect to histomorphology, distribution of CD4 and CD8 cells, and localization of proliferating and apoptotic cells, and to examine the morphology of M cells and expression of CK18 in follicle associated epithelium (FAE). We also hypothesized that CK18 could be a potential marker for M cell. Read More

Acta Histochem 2019 Apr 4;121(3):303-310. Epub 2019 Feb 4.

Department of Experimental and Clinical Physiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, Banacha 1b, 02-097, Warsaw, Poland.

It is well known that obesity contributes to the development of systemic inflammatory responses, which in turn may be involved in the process of interstitial fibrosis and left ventricular (LV) remodelling. Activation of pro-inflammatory factors such as transforming growth factor β (TGF-β) can directly stimulate mitogen-activated protein kinase (MAPK) p38 and JNK. The aim of the study was to evaluate the level of TGF-β and MAPK p38 and JNK in the LV in Sprague Dawley (SPRD) rats maintained on a high fat diet (HFD). Read More

Acta Histochem 2019 Apr 2;121(3):297-302. Epub 2019 Feb 2.

Institute of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, PR China. Electronic address:

Heat stress damaged spermatogenesis and semen quality, however, the exact molecular mechanism is not clear. The objective of this study was to determine the effects of elevated ambient temperature and local testicular heating on the expressions of heat shock protein 70 and androgen receptor in boar testes. A growing body of evidence demonstrated that germ cell apoptosis can be aggravated by heat stress and androgen deprivation, and at normal temperature, withdrawal of androgen led to germ cell apoptosis. Read More

Acta Histochem 2019 Apr 31;121(3):289-296. Epub 2019 Jan 31.

Eskisehir Osmangazi University, Medicine School, Histology and Embryology Department, Eskisehir, Turkey.

Aim: The aim of this study was to investigate the effects of mirtazapine, which is anti-oxidative and antidepressant agent, on the kidney damage caused by diabetes mellitus.

Materials And Methods: The rats were randomly divided into three groups (n = 7 animals in each group). The group I rats served as control and they received 0. Read More

Acta Histochem 2019 Apr 21;121(3):277-283. Epub 2019 Jan 21.

Universidad de Buenos Aires, Facultad de Cs Veterinarias, Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Argentina. Electronic address:

A detailed description of the tegument and parotoid glands of pre-metamorphic, post-metamorphic, juvenile and adult individuals of Rhinella bergi is presented to provide an exhaustive analysis of the integumentary characteristics of this species. Fragments of the tegument were fixed in Bouin solution and preserved in buffered Formol 10%. Subsequently, scanning electron microscopy (SEM) was performed to characterize the macroscopic structure of these regions. Read More

Acta Histochem 2019 Apr 22;121(3):284-288. Epub 2019 Jan 22.

Department of Traditional Chinese Medicine, The Center Hospital of Karamay City, Karamay, 834000, Xinjiang, China. Electronic address:

Chloride channel 3 (CIC-3) has been suggested to be implicated in the carcinogenesis though; it still remains ill understood in hepatocarcinoma, especially in terms of clinicopathological meaning of its expression. Given this, herein, to understand the clinicopathological significance of CIC-3 expression in hepatocarcinoma, Immunohistochemistry was performed to examine the level of CIC-3, followed by statistical analysis of the correlation between expression versus clinicopathological variables, including gender, age, TNM classifications, tumor size, lymph node metastasis and overall prognosis. It was shown that positive staining of CIC-3 can be present in both hepatocarcinoma and its paired normal controls; and that CIC-3 was significantly over-expressed in hepatcarcioma on the whole relative to paired normal controls. Read More

Acta Histochem 2019 Apr 12;121(3):268-276. Epub 2019 Jan 12.

Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Neuroscience Paris - Seine, Institut de Biologie Paris Seine, 75005, Paris, France.

Dp71 is the major form of dystrophins (Dp) in the supraoptic nucleus (SON) and in the neural lobe of hypophysis (NL/HP). Dp71-null mice exhibit a hypo-osmolar status attributed to an altered osmosensitivity of the SON and to a perturbed vasopressinergic axis. Because oxytocin (OT) is implicated in osmoregulation via natriuresis, this study explored the oxytocinergic axis in Dp71-null mice after salt-loading (SL). Read More

Acta Histochem 2019 Apr 11;121(3):261-267. Epub 2019 Jan 11.

Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand. Electronic address:

Because of the wide variety of cells which are arrayed in the tissue of the gastrointestinal tracts of these two fish, basic histological examination is not appropriate to clarify the histological structure. But histochemical techniques can be applied to identify the chemical differences contained in the tissues. The present study aimed to compare the histochemical characteristics of the gastrointestinal tracts of adult fishes which consume different types of food: the Nile tilapia is herbivore and the hybrid catfish is carnivore. Read More

Acta Histochem 2019 Feb 5;121(2):240-247. Epub 2019 Jan 5.

Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str 1A, 10-719 Olsztyn-Kortowo, Poland.

Pregnancy-associated glycoproteins (PAGs) are abundant embryo-originated products expressed in the pre-placental trophoblast and, later, in the post-implantational chorionic epithelium of some mammalian species. This paper describes the identification and cellular immunolocalization of the chorionic PAG family in the discoidal-type placenta of the brown bear (Ursus arctos L. - Ua), in which the PAGs were named 'UaPAG-Ls'. Read More

Acta Histochem 2019 Feb 5;121(2):234-239. Epub 2019 Jan 5.

School of Marine Biosciences, Kitasato University, Sagamihara, Kanagawa 252-0373, Japan.

The gonadotropin-releasing hormone (GnRH) gene sequence has been identified in an annelid polychaete marine worm using continual genome sequencing. The distribution of GnRH immunoreactive (ir) cell bodies and fibers in the nerve ganglion of the clam worm Perinereis aibuhitensis (Polychaeta) was examined by immunohistochemistry using a newly produced rabbit polyclonal antibody raised against the marine worm GnRH (mwGnRH). The specificity of the antibody was confirmed by dot blot assay. Read More

Acta Histochem 2019 Feb 3;121(2):253-259. Epub 2019 Jan 3.

Key Laboratory of System Bio-medicine of Jiangxi Province, Jiujiang University, Jiujiang, 332000, PR China. Electronic address:

Understanding the mechanisms of adipogenic differentiation may lead to the discovery of novel therapeutic targets for obesity. The natural plant polyphenol compound curcumin can improve obesity-associated inflammation and diabetes in obese mice. The role of curcumin in adipogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs) is still unclear. Read More

Acta Histochem 2019 Feb 31;121(2):227-233. Epub 2018 Dec 31.

Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, 12120, Thailand; Department of Forensic Sciences, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, 12120, Thailand. Electronic address:

Dye extracts from plants have been valuable not only for the economy but also environmental sustainability. There have been many reports on the utilization of natural dyes extracted from various sources for staining of biological tissues. This study aimed to investigate the extraction of natural dye from black rice (Oryza Sativa), butterfly pea (Clitoria ternatea), fresh roselle (Hibiscus sabdariffa), and mulberry (Morus alba) to stain of human spermatozoa for morphology assessment. Read More

Acta Histochem 2019 Feb 28;121(2):218-226. Epub 2018 Dec 28.

Equipe de Neurochimie, LBPO, Faculté des Sciences Biologiques, USTHB, Alger, Algeria.

Dystrophins (Dps) are the sub-membranous proteins that work via the dystrophin-associated proteins complex, which comprises β-dystroglycan (β-DG), a cell surface receptor for extracellular matrix. Recently, we have revealed β-DG decrease and central function impairment of supraoptic nucleus (SON) in Dp71 deficient adult mice, opening the question on the profiles of Dps and β-DG during SON development. At birth and the age of 10, 20 and 60 days, we examined the expression by RT-PCR and Western-blotting, and the distribution by immunohistochemistry of Dps and β-DG. Read More

Acta Histochem 2019 Feb 24;121(2):207-217. Epub 2018 Dec 24.

Department of Anatomy, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt. Electronic address:

The increasing application of carbon nanotubes (CNTs) within environmental, occupational and consumer settings has raised concerns regarding their biosafety and adverse effects on human health. The present study was designed to investigate the possible adverse effect of pristine and functionalized (amylated and polyethelene glycol coated) multi-walled (MW) CNTs on rat kidney with special concern to the histological alterations and the associated oxidative stress, apoptosis and inflammation. Healthy male albino rats (n = 40) were randomly divided into 4 groups: group I (control), group II (pristine MWCNTs), group III (amylated MWCNTs) and group IV [polyethelene glycol (PEG)-coated MWCNTs]. Read More

Acta Histochem 2019 Feb 25;121(2):189-197. Epub 2018 Dec 25.

Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USA.

The aim of this study was to investigate the role of the nitric oxide (NO) system in ovarian function, by determining if arginine (Arg) supplementation impacts follicle number, cell proliferation, and expression of the NO system members in nutritionally compromised ewes. Ewes were randomly assigned into maintenance (C, 100% requirements), excess (O; 2xC), or restricted (U; 0.6xC) diets 8 weeks prior to Arg treatment. Read More

Acta Histochem 2019 Feb 24;121(2):198-206. Epub 2018 Dec 24.

Department of Biochemistry, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt. Electronic address:

The present study was conducted to evaluate the ameliorative effects of Allium sativum (garlic) as well as Spirulina maxima on lead acetate toxicity in rat testes. Forty adult, male, albino rats were divided into four groups (10 rats/each): group I served as the control; group II contained rats that received lead acetate (100 mg/kg); group III contained rats that received both lead acetate (100 mg/kg) and garlic (600 mg/kg); and group IV contained rats that received both lead acetate (100 mg/kg) and spirulina (500 mg/kg). All treatments were performed daily for one month. Read More

Acta Histochem 2019 Feb 19;121(2):182-188. Epub 2018 Dec 19.

Department of Zoology, Mizoram University, Aizawl, Mizoram 796 004, India. Electronic address:

Dexamethasone, a synthetic glucocorticoid has been used as an immunosuppressive and anti-inflammatory and affects reproduction. It has been suggested that testicular steroidogenesis involves PGC-1α and visfatin as key regulators. Previous studies have shown that dexamethasone down-regulates PGC-1α and visfatin expression in muscle and mammary epithelial cells respectively. Read More

Acta Histochem 2019 Feb 17;121(2):171-181. Epub 2018 Dec 17.

Cadi Ayyad University, Faculty of Sciences Semlalia, Neurosciences, Pharmacology and Environment Unit, Marrakesh, Morocco. Electronic address:

Lead (Pb) is a metal element released into the atmosphere and a major source of environmental contamination. The accumulation and concentration of this metal in a food web may lead to the intoxication of the body, more precisely, the nervous system (NS). In addition, Pb-exposure can cause structural and functional disruption of the NS. Read More

Acta Histochem 2019 Feb 15;121(2):156-163. Epub 2018 Dec 15.

Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand; Faculty of Allied Health Sciences, Burapha University, Chonburi, 20131, Thailand. Electronic address:

The giant freshwater prawn, Macrobrachium rosenbergii, is an important aquaculture species. A better understanding of the molecular components of reproduction in this species would help to advance the prawn production. In the present study, we demonstrated the presence of an egg laying hormone (ELH)-like peptide in the male reproductive system. Read More

Acta Histochem 2019 Feb 15;121(2):164-170. Epub 2018 Dec 15.

Department of Pathology, Kurume University School of Medicine, Kurume, Japan.

The latest research suggests cells in the maculae flavae located at both ends of the lamina propria of the human vocal fold mucosa have stemness. This study investigated the differentiation potential of the cells in the maculae flavae of the human vocal fold mucosa. Four normal human adult vocal folds from surgical specimens were used. Read More

Acta Histochem 2019 Feb 6;121(2):151-155. Epub 2018 Dec 6.

Department of Animal Physiology and Reproduction, University of Rzeszow, Werynia 502, 36-100, Kolbuszowa, Poland.

It is suggested that due to the several stomach modifications, Otocinculus affinis (dwarf sucking catfish) possess the ability to breathe air during hypoxia, however, the exact mechanism remains unknown. The aim of this study was detailed analysis of the expression of EGFR in the stomach and diverticulum of the O. affinis at the mRNA and protein levels together with the immunohistochemical localization of EGFR in these organs. Read More

Acta Histochem 2019 Feb 27;121(2):143-150. Epub 2018 Nov 27.

Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Faculty of Allied Health Science, Burapha University, Chonburi 20131, Thailand.

The mud crab, Scylla olivacea, is a high value economic marine animal in Thailand. However, collection of these crabs from natural habitat for local consumption and export has caused rapid population decline. Hence, aquaculture of this species is required and to this measure understanding of endocrine control of their reproduction must be understood. Read More

Acta Histochem 2019 Jan 25;121(1):43-49. Epub 2018 Oct 25.

Medipol International Health Center, In Vitro Fertilization Unit (IVF), İstanbul, Turkey. Electronic address:

Purpose Radiotherapy, chemotherapy, various tumors and invasive surgery can result in ejaculatory dysfunction and testicular insufficiency. Sperm cryopreservation is the only method which can provide a baby for couples. Cryopreservation freezes tissues and cells, allowing them to be stored for future use by stopping all biological activities. Read More

Acta Histochem 2019 Feb 24;121(2):248-252. Epub 2018 Nov 24.

Department of Experimental and Clinical Biomedical Sciences "Mario Serio", Viale Pieraccini 6, 50139, Florence, Italy.

Cell staining techniques are well established in cell biology and associated with a broad range of dedicated dyes; however, they are accompanied by non-negligible costs, preparation time and unavoidable alterations of the sample with foreign molecules. In this context, we point out and propose the use of darkfield microscopy (DM) combined with different fixation protocols (to be used anyway) to enhance the different cell structures and districts as a timesaving and inexpensive support to the techniques that need staining or immuno-staining protocols and products. In a first step, we have analysed the effect of different fixation protocols on DM images for various human cellular lines. Read More

Acta Histochem 2019 Feb 23;121(2):135-142. Epub 2018 Nov 23.

Department of Orthodontic Science, Department of Orofacial Development and Function, Division of Oral Health Science, Graduate School, Tokyo Medical and Dental University (TMDU), Tokyo, Japan.

Nasal obstruction causes mouth breathing, and affects the growth and development of craniofacial structures, muscle function in the stomatognathic system, and the taste perceptive system. However, the detailed mechanism underlying the effects of nasal obstruction on taste perception has not been fully elucidated. In this study, we investigated this mechanism using the two-bottle taste preference test, immunohistological analysis, and quantification of the mRNA expression of taste-related molecules in the circumvallate papillae. Read More

Acta Histochem 2019 Feb 18;121(2):101-112. Epub 2018 Nov 18.

Department of Structural and Functional Biology, Institute of Biology, University of Campinas (Unicamp), 13083-862, Campinas, SP, Brazil. Electronic address:

Toluidine blue (TB) staining either alone or in association with other methodologies has the potential to answer a variety of biological questions regarding the human, animal and plant tissues or cells. In this brief review, we not only report the primary use of TB to detect the anionic substrates and availability of their binding sites, but also unveil the resulting applications of TB staining in biological research. Among these applications, the uses of TB staining to identify the changes in chromatin DNA-protein complexes, nucleolus location, and extracellular matrix proteoglycan complexes associated with different physiological and pathological events are described. Read More

Acta Histochem 2019 Feb 16;121(2):125-134. Epub 2018 Nov 16.

Department of Medical Biology, Cancer Center Amsterdam, Amsterdam UMC at the Academic Medical Center, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands; Department of Genetic Toxicology and Cancer Biology, National Institute of Biology, Večna Pot 111, 1000 Ljubljana, Slovenia. Electronic address:

Immunohistochemistry (IHC) specifically localizes proteins in cells and tissues, but methodologies vary widely. Therefore, we performed a methodological IHC optimization and validation study. First, we compared advantages and disadvantages of cryostat sections versus paraffin sections. Read More

Acta Histochem 2019 Feb 15;121(2):119-124. Epub 2018 Nov 15.

Instituto de Histologia e Embriologia, Faculdade de Medicina, Universidade de Coimbra, Polo I Rua Larga, 3004-504, Coimbra, Portugal; Hospitais da Universidade de Coimbra, Av. Bissaya Barreto Praceta Prof. Mota Pinto, 3000-075 Coimbra, Portugal.

Mammalian lung development is a complex process that is partially accomplished during the postnatal period. Surface carbohydrates are crucial in many biological and pathological phenomena and are key partners during development. The outer surface of lung epithelial cells, which is rich in carbohydrate components, plays a pivotal role throughout the developmental process. Read More

Acta Histochem 2018 11;120(8):859

Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.

Acta Histochem 2018 11;120(8):858

Department of Biosciences and Territory, University of Molise, Contrada Fonte Lappone, 86090 Pesche (Isernia), Italy. Electronic address: