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Acta Histochem 2021 Jun 8;123(5):151737. Epub 2021 Jun 8.

Institute of Animal Husbandry and Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China. Electronic address:

Early weaning is usually applied to improve the reproductive efficiency of sheep in mutton production, while the development of rumen is of vital importance for sheep weaning age. Translationally controlled tumor protein (TCTP) is a highly conserved protein which participates in multiple tissue and organ development. Thus, we hypothesized that TCTP was involved in sheep rumen development. Read More

Acta Histochem 2021 Jun 8;123(5):151740. Epub 2021 Jun 8.

Department of Anatomy, Histology and Embryology, University of Split School of Medicine, Split, Croatia. Electronic address:

Aim: One of the main causes of end-stage renal disease (ESRD) in the world is IgA nephropathy (IgAN). Since kidney is a key player in vitamin D metabolism, we investigated the expression of renal vitamin D receptors (VDR) and metabolizing enzymes in IgA nephropathy patients (IgAN-P).

Methods: The sample included twelve IgAN-P who underwent ultrasound-guided renal biopsies and five controls who underwent nephrectomy due to clear renal carcinoma. Read More

Acta Histochem 2021 Jun 6;123(5):151739. Epub 2021 Jun 6.

Department of Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. Electronic address:

Resveratrol is a kind of iPolyphenols widely existing in herbal medicine. Here we aim to investigate whether resveratrol can reduce the degree of myocardial ischemia/reperfusion (IR) injury and inhibit the development of oxidative stress, and elucidate the molecular mechanism of resveratrol in protecting myocardial cells. The primary rat cardiomyocytes were used to establish an ischemia/reperfusion model in vitro, and a series of routine biochemical experiments were conducted to explore the antioxidant and anti-apoptotic effects of resveratrol in myocardial ischemia-reperfusion injury. Read More

Acta Histochem 2021 Jun 3;123(5):151738. Epub 2021 Jun 3.

Department of Biology, University of Ottawa, 30 Marie Curie Pvt., Ottawa, ON, K1N 6N5, Canada. Electronic address:

Transport epithelia maintain the volume, ion concentration and acid-base balance of blood and extracellular fluids. In teleost fish, mitochondrion-rich cells (MRCs) are specialized ionocytes that perform this role. These cells are found in epithelia of the gills and buccal surface of the operculum (the bony structure covering the gills). Read More

Acta Histochem 2021 May 31:151720. Epub 2021 May 31.

Department of Molecular Medicine, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.

Transplantation of bone marrow stem cells (BMSCs) has shown to have a vital role in promoting nerve regeneration after SCI. The aim of this study was to investigate the effect of BMSCs transplantation in healing of spinal cord injury (SCI) in mice based on morphologic parameters. Forty two male mice were randomly divided into 3 groups of control with no intervention, experimental SCI without treatment, and experimental SCI transplanted with 2 × 10 BMSCs intravenously. Read More

Acta Histochem 2021 May 29;123(5):151736. Epub 2021 May 29.

Key Laboratory of Stem Cells and Gene Drug of Gansu Provincial, The 940(th) Hospital of Joint Logistics Support Force of Chinese People's Liberation Army, Lanzhou, Gansu, 730070, China.

Lappaconitine hydrochloride (LH), as a new synthetic alkaloid, exhibits antitumor activity, whereas its antitumor effect on colorectal cancer (CRC) has not been investigated. In this study, the effect of LH on HCT-116 cell proliferation and apoptosis in vivo and in vitro and underlying molecular mechanism were explored. The Cell Counting Kit-8 (CCK-8) was used to assess cell viability. Read More

Acta Histochem 2021 May 27;123(5):151733. Epub 2021 May 27.

Department of Orthodontics, Dongfeng Stomatological Hospital, Hubei University of Medicine, Shiyan, 442000, Hubei Province, China. Electronic address:

Objective: To explore the role of RN181 in the pathogenesis of oral squamous cell carcinoma (OSCC) cells via mediating ERK/MAPK signaling.

Methods: The expression of RN181 was detected in OSCC tissues and cells. CAL27 and SCC-15 cells were divided into Control, Empty, RN181, si-RN181, U0126 (an inhibitor of ERK/MAPK pathway) and si-RN181 + U0126 groups. Read More

Acta Histochem 2021 May 27;123(5):151731. Epub 2021 May 27.

Department of Anatomy, Faculty of Medicine, Mansoura University, Egypt; Department of Anatomy, Faculty of Medicine, Taibah University, Madinah, Saudi Arabia.

Iron deficiency anemia (IDA) is a global health problem affecting various body systems and tissues including the cardiovascular system. Several literatures described the associated physiological and clinical changes in the cardiovascular system and heart. However, the associated structural changes were poorly investigated. Read More

Acta Histochem 2021 May 27;123(5):151735. Epub 2021 May 27.

Department of Biomedical Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620 024, India. Electronic address:

Hyperglycemia-induced oxidative stress has been implicated in diabetes and its complications. Medicinal plants possessing antioxidant activity may decrease oxidative stress by scavenging radicals and reducing power activity and would be a promising strategy for the treatment of inflammatory disorders like diabetes. This study was designed to evaluate the antioxidant effect of Aqueous Extract of  S. Read More

Acta Histochem 2021 May 25;123(5):151728. Epub 2021 May 25.

Department of Anesthesiology, Hunan Cancer Hospital, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410013, Hunan Province, PR China. Electronic address:

Background: As widely reported, propofol can effectively inhibit tumors development. However, little is known about the molecular mechanisms. Here, we proved that propofol regulated miR-340/CDK2 axis to suppress bladder cancer progression in vitro. Read More

Acta Histochem 2021 May 25;123(5):151734. Epub 2021 May 25.

Department of Anesthesiology, The First Affiliated Hospital of Nanchang University, Nanchang, China.

Rat hippocampal neurons were isolated and divided into Normal, oxygen glucose deprivation/reoxygenation (OGD/R), OGD/R + DEX, OGD/R + NC mimic, OGD/R + miR-155 mimic and OGD/R + DEX + miR-155 mimic groups. In OGD/R group, LDH, ROS and MDA levels and apoptosis rate was increased, with up-regulations of miR-155, Cyt c and Bax/Bcl-2 ratio, but decreases of SOD, GSH-Px and MMP levels, as well as down-regulations of p-ERK1/2/ERK1/2. As compared to the OGD/R group, parameters above in the OGD/R + DEX group were ameliorated evidently, while OGD/R + miR-155 mimic group manifested the opposite changes. Read More

Acta Histochem 2021 May 18;123(5):151732. Epub 2021 May 18.

Department of Pharmacology, University of Zagreb School of Medicine, Zagreb, Croatia; Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia.

Acta Histochem 2021 May 6;123(5):151717. Epub 2021 May 6.

Department of Radiobiology, National Atomic Energy Commission of Argentina (CNEA), Argentina; National Research Council of Argentina (CONICET), Av. General Paz 1499 (1650), San Martín, Buenos Aires, Argentina. Electronic address:

The Na/H exchange regulatory factor 1 or Ezrin-radixin-moesin-binding phosphoprotein 50 (NHERF1/EBP50) is an adaptor protein implicated in the stabilization of molecular complexes linking extracellular signals with the cytoskeleton machinery. NHERF1 expression at the cell cortex is associated with the maintenance of adherent junction integrity in polarized epithelia. The role of NHERF1 in cancer depends on its localization within the cell, acting, in most cases, as a tumor suppressor when localized at the cell membrane, and as an oncogene, when expressed in the cytoplasm or the nucleus of cancer cells. Read More

Acta Histochem 2021 May 4;123(5):151719. Epub 2021 May 4.

Department of Histology and Embryology, School of Medicine, University of Mostar, Bijeli Brijeg bb, 88000, Mostar, Bosnia and Herzegovina.

Aim of our study was to provide insight into the temporal and spatial expression of FGFR1, FGFR2 and CTGF during normal human lung development which may have an important impact on understanding occurrence of developmental lung anomalies. Morphological parameters were analysed using double immunofluorescence on human embryonal (6th and 7th developmental week-dw) and foetal (8th, 9th and 16th developmental week) human lung samples. FGFR1 and FGFR2 was positive during all the dw in both the epithelium and mesenchyme. Read More

Acta Histochem 2021 May 5;123(5):151718. Epub 2021 May 5.

Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, 56 Lingyuan West Road, Guangzhou, Guangdong, 510055, China; Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China; Guangdong Engineering Research Center of Technology and Materials for Oral Reconstruction, Guangzhou, China. Electronic address:

Aging, which has become a worldwide problem, leads to the degeneration of multiple organs and tissues. Two of the main changes in aging are dysregulation of the tissue microenvironment and abnormal functioning of specific stem cells. Bone marrow stem cells (BMSCs) in the aging microenvironment are not only effector cells but also immunomodulatory cells that change the microenvironment. Read More

Acta Histochem 2021 May 30;123(4):151715. Epub 2021 Apr 30.

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy.

Ulcerative colitis is a chronic inflammatory condition of the gastrointestinal tract that can affect people of worldwide. In contrast with Crohn's disease, that can relate the entire thickness of the bowel wall, the inflammation of ulcerative colitis is limited to the colonic mucosa. Immune cells including activated T cells, plasma cells, mast cells, macrophages, and dendritic cells (DCs) trigger the inflammation. Read More

Acta Histochem 2021 May 29;123(4):151716. Epub 2021 Apr 29.

Department of Histology and Developmental Biology, Pomeranian Medical University, Żołnierska 48, 71-210, Szczecin, Poland.

Cancer cell lines are widely used as in vitro models to elucidate biological processes in cancer, and as a tool to evaluate anticancer agents. In fact, the use of an appropriate cancer cell line in cancer research is crucial for investigating new, potential factors involved in carcinogenesis. One of them is aquaporin-3 (AQP3), which is a small, hydrophobic, integral membrane protein with a predominant role in water and glycerol transport. Read More

Acta Histochem 2021 May 21;123(4):151713. Epub 2021 Apr 21.

Department of Physiology, Christian Medical College, Vellore, 632002, India. Electronic address:

Introduction: Chondroprogenitors, a promising therapeutic modality in cell-based therapy, are routinely isolated from articular cartilage by fibronectin differential adhesion assay. However, there is paucity of information regarding their biological profile and the lack of a marker that can reliably distinguish them from cultured chondrocytes due to possible dedifferentiation. Since chondroprogenitors have been classified as mesenchymal stem cells(MSCs), the aim of our study was to compare bone marrow-MSCs, chondroprogenitors and chondrocytes, and assess superiority for cartilage repair. Read More

Acta Histochem 2021 May 12;123(4):151714. Epub 2021 Apr 12.

Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros s/n, Dois Irmãos, 52171-900, Recife, PE, Brazil.

Several studies have demonstrated the effects of essential oils on insect pests. These effects vary and affect fundamental parameters for the survival of these organisms. However, there is a scarcity of research on the effect of these substances on Alabama argillacea (Hübner) (Lepidoptera: Erebidae), the main defoliating pest of cotton (Gossypium hirsutum L. Read More

Acta Histochem 2021 May 10;123(4):151712. Epub 2021 Apr 10.

Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address:

Scrotal hyperthermia leads to altered spermatogenesis due to heat-related oxidative stress. One of the main causes of infertility in men is oxidative stress, which refers to an imbalance in the levels of reactive oxygen species (ROS) and antioxidants. Therefore, this study aimed to evaluate the effects of chronic scrotal hyperthermia on testicular tissue structure, sperm parameters, and oxidative stress in adult mice. Read More

Acta Histochem 2021 May 7;123(4):151711. Epub 2021 Apr 7.

Institute for Hematopathology, Hamburg, Germany.

To increase the efficiency of interpretation of mast cell's contribution to the state of a specific tissue microenvironment, it is necessary to detail the molecular composition of their secretome and analyze the pathways of degranulation. Developed method of combining immunomorphological and histochemical staining protocols contributes to the most objective detection of the integral level of tryptase expression in the intraorgan population of the skin mast cells. Novel technique for tryptase detection expands the possibilities of morphological analysis, provides researchers with additional data on the structure of the mast cell population and helps visualize the processing and cytological features and structural targets of tryptase during the development of adaptive and pathological reactions. Read More

Acta Histochem 2021 May 13;123(4):151710. Epub 2021 Mar 13.

College of Medicine, The Ohio State University, Columbus, OH, USA. Electronic address:

Antibodies are essential tools in modern science and medicine, however the history leading to the use of antibodies as tools has not been well-described. The objective of this paper is to analyze the history of immunology from smallpox inoculation to the production of monoclonal antibodies, and to identify turning points in immunological theory leading to the emergence of antibody-tools. In the early 1700's, Western medicine adopted smallpox inoculation from Turkey, along with the idea of acquired immunity. Read More

Acta Histochem 2021 May 9;123(4):151709. Epub 2021 Mar 9.

Istanbul Aydin University, Faculty of Medicine, Department of Medical Biology and Genetics, Istanbul, Turkey. Electronic address:

Current evidence strongly suggests that aberrant activation of the nuclear factor kappa B (NF-kB) signaling cascade is connected to carcinogenesis. The matrix metalloproteinases (MMP) which are also the key agents for tumor metastasis may be potent candidates for tumor diagnosis in clinics. In this in vitro study, we hypothesized that metformin with an effective dose can inhibit tumor cell proliferation and metastasis by modulating the expressions of MMP-2 and -9 and interfering with NF-kB signaling in primary breast cancer cells (PBCCs). Read More

Acta Histochem 2021 May 10;123(4):151698. Epub 2021 Mar 10.

School of Biology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, Thailand. Electronic address:

Maternal care behaviors in birds include incubation and rearing behaviors. During incubating period, the hens stop laying and eating less due to food restriction as a natural fasting when compared with the rearing hens, resulting in low production of eggs and chicks. Neuropeptide Y (NPY), a neurotransmitter/neuromodulator, is very well known to be involved in food intake regulation in birds and mammals. Read More

Acta Histochem 2021 May 7;123(4):151701. Epub 2021 Mar 7.

Division of Plastic Surgery and Hand Surgery, University Hospital Zurich, Sternwartstrasse 14, Zurich, 8091, Switzerland. Electronic address:

Pre-clinical animal models are needed to investigate and study kidney injuries and diseases. The rabbit kidney model is frequently used because various important parameters can be assessed with it. For example, histology and immunohistochemistry are indispensable as tissue morphology and composition can be investigated qualitatively as well as quantitatively. Read More

Acta Histochem 2021 Apr 2;123(3):151700. Epub 2021 Mar 2.

Cellular and Molecular Research Center, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran. Electronic address:

Background: Thyroid carcinoma is the most common endocrine malignancy and anaplastic thyroid carcinoma (ATC) is a rare but most aggressive cancer. Melatonin has enhanced or induced apoptosis in many different cancer cells, however, there has not been any study on the effects of melatonin in the treatment of ATC. In this study, we examined the effect of melatonin on cytotoxicity in the human ATC cell line. Read More

Acta Histochem 2021 Apr 1;123(3):151697. Epub 2021 Mar 1.

Center of Biological and Health Science, UFOB - Universidade Federal do Oeste da Bahia, Barreiras, Bahia, Brazil. Electronic address:

Responsible for post-testicular maturation, concentration, protection and sperm storage, the epididymis is an organ that can be easily subdivided into three segments: caput, corpus and cauda. Each epididymal region displays different morphology and functions within the sperm maturation process. Despite the great importance of this organ, studies on its morphology and hormonal control in bats remain scarce. Read More

Acta Histochem 2021 Apr 1;123(3):151699. Epub 2021 Mar 1.

Department of Surgery, Tufts Medical Center, 800 Washington Street, Boston, MA, 02111, USA. Electronic address:

In this project, the ability of dual growth factor-preloaded, silk-reinforced, composite hyaluronic acid-based hydrogels to elicit advantageous histologic responses when secured to ischemic myocardium was evaluated in vivo. Reinforced hydrogels containing both Vascular Endothelial Growth Factor (VEGF) and Platelet-derived Growth Factor (PDGF) were prepared by crosslinking chemically modified hyaluronic acid and heparin with poly(ethylene glycol)-diacrylate around a reinforcing silk mesh. Composite patches were sutured to the ventricular surface of ischemic myocardium in Sprague-Dawley rats, and the resulting angiogenic response was followed for 28 days. Read More

Acta Histochem 2021 Apr 27;123(3):151696. Epub 2021 Feb 27.

Department of Otorhinolaryngology, Children's Hospital of Nanjing Medical University, No.72 Guangzhou Road, Nanjing, 210008, Jiangsu, China.

Sensorineural hearing loss is a health problem with global prevalence. Aminoglycoside antibiotics, for instance gentamicin, may cause ototoxicity in mammals as a result of apoptosis and elevated oxidative stress in cochlear hair cells. Our study aimed to examine the potential effects of theophylline, an HDAC2 agonist, on gentamicin-induced cytotoxicity to sensory hair cells. Read More

Acta Histochem 2021 Apr 16;123(3):151693. Epub 2021 Feb 16.

Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:

In human, there is substantial neurogenesis in the hippocampus that is implicated in memory formation and learning. These new-born neurons can be affected by neuropathological conditions. Anesthesia and surgical procedures are associated with postoperative cognitive changes particularly, impaired memory and learning. Read More