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Acta Histochem 2022 Jun 28;124(6):151926. Epub 2022 Jun 28.

Department of Orthopedics, the First Hospital of Nanchang, Nanchang, Jiangxi 330008, PR China.

This study was conducted to investigate the impact of the microRNA (miR)-25-3p/ITGB3 axis on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) from patients with osteoporosis (OP). BMSCs isolated from the bone marrow of healthy controls and OP patients were identified by flow cytometry, in which ITGB3 mRNA and miR-25-3p expression was detected by RT-qPCR and ITGB3, Runx2, OPN, ALP, and OSX protein expression by western blot. The binding between ITGB3 and miR-25-3p was assessed by dual-luciferase reporter gene and Ago2-RIP assays. Read More

Acta Histochem 2022 Jun 27;124(6):151919. Epub 2022 Jun 27.

Anhui Province Key Laboratory of Translational Cancer Research, Bengbu Medical College, Anhui 233030, China; Department of Life Sciences, Bengbu Medical College, Anhui 233030, China. Electronic address:

Pancreatic cancer is one of the deadliest cancers in the world, causing hundreds of thousands of deaths worldwide annually. Because of late diagnosis, rapid metastasis and drug resistance to chemotherapy, pancreatic cancer has a poor prognosis. Although the treatment of pancreatic cancer has made tremendous progress, the options for effective treatment are still limited, and new treatment methods are in crying needs to improve prognosis in clinic. Read More

Acta Histochem 2022 Jun 25;124(6):151911. Epub 2022 Jun 25.

Senior Department of Orthopedics, the Fourth Medical Center of PLA General Hospital, No. 51 Fucheng Road, Beijing, China. Electronic address:

Background: The prevalence of osteoarthritis (OA) is constantly increasing with age. Adipose-derived (AD-) and umbilical cord-derived (UC-) mesenchymal stem cells (MSCs) are attractive alternatives in OA therapy and regenerative medicine. However, whether there are differences in the efficacy of MSCs derived from different tissues in the cartilage regeneration, and the frequency of administration of MSCs needs to be further studied. Read More

Acta Histochem 2022 Jun 24;124(6):151913. Epub 2022 Jun 24.

Departement of Oral and Maxillofacial Surgery, College of Stomatology, Guangxi Medical University, Nanning 530021, People's Republic of China; Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Guangxi Clinical Research Center for Craniofacial Deformity, Nanning 530021, People's Republic of China. Electronic address:

Distraction osteogenesis (DO) is a widely used surgical technique to repair bone defects, partly owing to its high efficiency in inducing osteogenesis; however, the process of osteogenesis is complex, and the precise mechanism is still unclear. Among the factors identified for an effective DO procedure, well-controlled inflammation is essential. We aimed to explore how microRNA(miR)-146a, a negative regulator of inflammation, influences osteogenesis in DO. Read More

Acta Histochem 2022 Jun 22;124(6):151916. Epub 2022 Jun 22.

Department of Preventive Medicine, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China; Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazardsa, Hengyang Medical School, University of South China, Hengyang, China. Electronic address:

The N6-methyladenosine (mA) refers to the methylation of the N6 position of adenosine of RNA adenine. The modification of mA is one of the most abundant epigenetic modifications in eukaryotic mRNA and non-coding RNA and is controlled by methyltransferases and demethylases. The biological mechanism and significance of mA have been discovered with the development of mA sequencing. Read More

Acta Histochem 2022 Jun 20;124(6):151915. Epub 2022 Jun 20.

Departamento de Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Cd. Universitaria, 04510 Coyoacán, Ciudad de México, Mexico. Electronic address:

Intraventricular and extraventricular choroid plexuses are neuroepithelial folds which arise from the roof of the diencephalon. We describe the circumventricular structure of the diencephalon roof (paraphysis cerebri) during the various development stages of Ambystoma mexicanum. The parasagittal sections of the larvae epithalamus exhibit the presence, in addition to the epiphysis, of two dorsal primordia in nearby areas, which appear to be extraventricular saccular evaginations of different origin that give rise to two structures we define as the anterior extraventricular choroid plexus (AEP) and posterior extraventricular choroid plexus (PEP). Read More

Acta Histochem 2022 Jun 17;124(6):151918. Epub 2022 Jun 17.

Department of Pediatrics, The First Hospital of Changsha, Hunan Province, China; Department of Neurosurgery, The First Hospital of Changsha, Hunan Province, China. Electronic address:

Background And Purpose: Hyperbilirubinemia is a common condition in neonates that is associated with poor neurodevelopmental outcomes. Although studies have proposed that calycosin has a neuroprotective effect, the exact molecular mechanism underlying calycosin treatment of hyperbilirubinemia remains elusive. To fill this gap, we analyzed the mechanism of calycosin treatment in hyperbilirubinemia model mice. Read More

Acta Histochem 2022 Jun 15;124(6):151917. Epub 2022 Jun 15.

Facultad de Química Farmacéutica Biológica, Universidad Veracruzana, Xalapa, Veracruz, Mexico. Electronic address:

Measurements of Morphometric Parameters of the Blood Cells (MPBC) are key for the diagnosis of both mental and metabolic diseases. Several manual approaches or computational methodologies are useful to provide reliable clinical diagnosis. The sample processing and data analysis is relevant, however the sample handling on the pre-analytical phase remains scarcely evaluated. Read More

Acta Histochem 2022 Jun 8;124(6):151914. Epub 2022 Jun 8.

College of Health and Human Sciences, Charles Darwin University, Darwin, Northern Territory 0909, Australia. Electronic address:

This descriptive study examined the variation in ventral Crocodylus porosus (saltwater crocodile) belly skin. The study is the first to determine both intra and inter variation of C. porosus skin, epidermis and dermis thickness across the ventral belly region. Read More

Acta Histochem 2022 Jul 6;124(5):151909. Epub 2022 Jun 6.

SC Laboratorio di Immunoreumatologia e Rigenerazione Tissutale, IRCCS Istituto Ortopedico Rizzoli, Via di Barbiano 1/10, Bologna 40136, Italy. Electronic address:

Objective: Immune cell evaluation could be useful for clarifying etiopathogenesis, providing a support for formulating the diagnoses of clinically similar joint pathologies or guiding indications for possible therapeutic targets. To contribute to differential diagnosis in joint pathologies we performed an immunophenotypical profile analyzing different immune cells in synovial tissues from patients with rheumatoid arthritis (RA) and osteoarthritis (OA).

Methods: The Krenn and immunologic synovitis (IMSYC) scores, which include the evaluation of T lymphocytes (CD3 positive), B lymphocytes (CD20), endothelial cells (CD31), macrophages (CD68) and proliferating cells (Ki-67 positive) were used to analyze the synovial tissue samples. Read More

Acta Histochem 2022 Jul 4;124(5):151910. Epub 2022 Jun 4.

Department of Biotechnology, Dr. M.G.R Educational and Research Institute, Deemed to be University, Chennai, India.

Gene expression changes are one of the hallmarks of malignant cells and such changes in specific genes have been identified for a variety of human cancers. Such an association in gene expression changes becomes very significant for breast cancers due to the genetic heterogeneity seen in such cancers. It is due to such genetic implication that breast cancers are classified into several subtypes; based on the expression and the magnitude of expression of estrogen and progesterone receptor genes. Read More

Acta Histochem 2022 Jul 25;124(5):151908. Epub 2022 May 25.

Cellular and Molecular Research Center,Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran; Department of Biochemistry, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran. Electronic address:

Cytokine storms and extra-activated cytokine signaling pathways can lead to severe tissue damage and patient death. Activation of inflammatory signaling pathways during Cytokine storms are an important factor in the development of acute respiratory syndrome (SARS-CoV-2), which is the major health problem today, causing systemic and local inflammation. Cytokine storms attract many inflammatory cells that attack the lungs and other organs and cause tissue damage. Read More

Acta Histochem 2022 Jul 1;124(5):151912. Epub 2022 Jun 1.

Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic. Electronic address:

Cell lines as an in vitro model for xenobiotic screening and toxicity studies provide a very important tool in the field of scientific research at the level of molecular pathways and gene expression. Good cell culture practice and intracellular characterization, as well as physiological properties of the cell line are of critical importance for in vitro reproductive toxicity testing of various endocrine-disrupting chemicals. The NCI-H295R, human adrenocarcinoma cell line, is the most widely used in vitro cellular system to study the human adrenal steroidogenic pathway at the level of hormone production and gene expression, as it expresses genes that encode for all the key enzymes for steroidogenesis. Read More

Acta Histochem 2022 Jul 25;124(5):151907. Epub 2022 May 25.

Human Anatomy & Embryology Department, Faculty of Medicine, Sohag University, Sohag 82524, Egypt.

Polyethylene glycol (PEG) is widely used polymer in the field of pharmaceutics, particularly in which related to drug delivery systems (DDS). Surface coating of the nanoparticles (NPs) with PEG (i.e. Read More

Acta Histochem 2022 Jul 14;124(5):151899. Epub 2022 May 14.

P. G. Department of Zoology, Maharaja Sriram Chandra Bhanja Deo University, Baripada 757003, Odisha, India.

Tail regression is a remarkable process where a complex organ like the tail is completely resorbed by cell death during anuran metamorphosis. Nitric oxide is a signalling molecule involved in various physiological processes and along with reactive nitrogen species induces apoptosis. The present study describes the contribution of nitric oxide and reactive nitrogen species (nitrosative stress) during tail regression in the tadpoles of Indian tree frog, Polypedates maculatus. Read More

Acta Histochem 2022 Jul 5;124(5):151898. Epub 2022 May 5.

Department of Cell and Systems Physiology, University of Occupational and Environmental Health School of Medicine, Kitakyushu 807-8555, Japan. Electronic address:

TWIK-related acid-sensitive K (TASK) channels are thought to contribute to the resting membrane potential in adrenal cortical (AC) cells. However, the molecular identity of TASK channels in AC cells have not yet been elucidated. Thus, immunocytochemical and molecular biological approaches were employed to investigate the expression and intracellular distribution of TASK1 and TASK3 in mouse AC cells and H295R cells derived from human adrenocortical carcinoma. Read More

Acta Histochem 2022 May 27;124(4):151881. Epub 2022 Apr 27.

Department of Cardiology, Wuhan Third Hospital (Tongren Hospital of Wuhan University), Wuhan 430061, Hubei, China. Electronic address:

Background: Visceral adipose tissue-derived serine protease inhibitor (Vaspin) was found to have anti-inflammatory, anti-apoptosis, and pro-autophagy activities. Our investigation is aimed to ascertain the effect of Vaspin on hypoxia-evoked endothelial-mesenchymal transition (EndMT) in human cardiac microvascular endothelial cells (HCMECs).

Methods: In vitro assays including CCK8, TUNEL, western blots, RT-qPCR to assess the effect of Vaspin on hypoxia-induced cell injuries, endothelial-mesenchymal transition (EndMT) and the inflammatory state in HCMECs. Read More

Acta Histochem 2022 May 26;124(4):151895. Epub 2022 Apr 26.

Department of Biochemistry, DKM College for Women, Vellore, Tamil nadu, India.

Cancer is a disease characterised by abnormal cell growth that can invade or spread to other regions of the body. Organoids are three-dimensional ex vivo tissue cultures made from embryonic stem cells, induced pluripotent stem cells, progenitor cells or tissue that serve as a physiological model for cancer research. These are designed to recapitulate the in vivo properties of tumours. Read More

Acta Histochem 2022 May 22;124(4):151897. Epub 2022 Apr 22.

Emergency Clinical County Hospital Cluj-Napoca, Romania; Iuliu Hațieganu University of Medicine and Pharmacy, Dept. of Histology, Cluj-Napoca, Romania.

Introduction: The pineal gland is a small photo-neuro-endocrine organ. This study used human post-mortem pineal glands to microscopically assess immunohistochemical marker intensity and percentage of positivity using known and novel digital techniques.

Materials And Methods: An experimental non-inferiority study has been performed on 72 pineal glands harvested from post-mortem examinations. Read More

Acta Histochem 2022 May 18;124(4):151894. Epub 2022 Apr 18.

Department of Genetics and Applied Toxicology, Lutheran University of Brazil, Canoas, RS, Brazil. Electronic address:

This study aimed to evaluate the efficacy and safety of a topical and oral administration of pumpkin seed oil (PSO) on the hair growth of BALB/c male mice. The animals had their dorsal area shaved (2 ×2 cm) and they were divided into 6 experimental groups. They received orally saline (OS), finasteride (F), or PSO (OP) for 14 days; or topically saline (TS), minoxidil (M), or PSO (TP) for 7 days. Read More

Acta Histochem 2022 May 14;124(4):151896. Epub 2022 Apr 14.

Anatomy department, Faculty of Medicine, Minia University, 61511 El-Minia, Egypt.

Gastric ulcer is a common frequent clinical problem affecting all age and gender. This work aims to compare between the therapeutic effects of stem cell derived exosomes, stem cells conditioned medium and omeprazole on the healing of gastric ulcer model. Fifty rats were, assigned into 5 groups; control, gastric ulcer, omeprazole-treated, conditioned medium- treated, and exosomes-treated groups. Read More

Acta Histochem 2022 May 11;124(4):151892. Epub 2022 Apr 11.

Laboratório de Biologia Estrutural e Funcional, Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Rua Universitária, 1619, CEP 85819-110 Cascavel, Paraná, Brazil.

Skeletal muscle histopathological changes induced or caused by pathologies in animal models, can impair functionality, being the main focus of therapeutic studies. This study aimed to propose a histopathological index to assess, in a quantitative manner, skeletal muscle changes induced by experimental protocols for Rodentia's models. For the development, evaluation of fit and parsimony, replicability, and sensitivity index, Wistar rats from experiments with the same experimental design, but with different variation factors, were used to achieve different levels of damage. Read More

Acta Histochem 2022 May 8;124(4):151893. Epub 2022 Apr 8.

Department of Anatomy, All India Institute of Medical Sciences, New Delhi, India. Electronic address:

Peripheral microcystoid retinal degeneration (PMD) is an age-related, benign condition in which the peripheral retina develops small holes and undergoes cystic degeneration. This paper demonstrates neuronal alterations in PMD, as studied by immunohistochemistry in postmortem donor eyes (age: 76-89 years; N = 6 donors). In all cases, the degeneration was located in the inferior temporal quadrant, creating holes in the far peripheral retina. Read More

Acta Histochem 2022 May 31;124(4):151891. Epub 2022 Mar 31.

Department of Rehabilitation, Faculty of Rehabilitation, Hiroshima International University, Kurose-Gakuendai 555-36, Higashi-Hiroshima, Hiroshima, Japan.

The accumulation of marrow adipose tissue (MAT) is frequently associated with bone loss. Although anterior cruciate ligament (ACL) injury induces bone loss, MAT accumulation after ACL injury has not been evaluated. In addition, no information about changes in MAT after ACL reconstruction is available. Read More

Acta Histochem 2022 May 31;124(4):151872. Epub 2022 Mar 31.

Henan Medical Key Laboratory of Arrhyrithmia, Zhengzhou No. 7 People's Hospital, Zhengzhou 450016, China. Electronic address:

Objective: This study aimed to investigate the protective role of the signal transducer and activator of transcription 4 (STAT4) in diabetic cardiomyopathy.

Materials And Methods: Male Sprague-Dawley (SD) rats (6-8 weeks old) were purchased from the Experimental Animal Center of Zhengzhou University. The rats were randomly divided into the control and diabetic cardiomyopathy groups. Read More

Acta Histochem 2022 May 30;124(4):151890. Epub 2022 Mar 30.

Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.. Electronic address:

Deep learning algorithms and artificial intelligence (AI) are making great progress in their capacity to evaluate and interpret image data recent advancements in computer vision and machine learning. The first use of AI in a pathology lab was in cytopathology, when a computer-assisted Pap test screening was created. Initially designed to diagnose rather than screen, there was a lot of disagreement concerning their wide use to clinical specimens. Read More

Acta Histochem 2022 May 28;124(4):151879. Epub 2022 Mar 28.

School of Basic Medical Sciences, Anhui Medical University, 81 Meishan Road, Hefei, Anhui, PR China. Electronic address:

Formalin-fixed, paraffin-embedded (FFPE) tissues have been widely used in researches. Proteins and nucleic acids in prolonged FFPE tissues display different degrees of degradation. We investigated the effect of prolonged formalin fixation on protein expression in human brain tissues. Read More

Acta Histochem 2022 May 25;124(4):151889. Epub 2022 Mar 25.

College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, No. 1 Mingxian South Road, Taigu 030801, China. Electronic address:

Acta Histochem 2022 May 26;124(4):151880. Epub 2022 Mar 26.

Department of Cell & Systems Biology, University of Toronto, 25 Harbord Street, Toronto, ON M5S 3G5, Canada.

The tissue processing technique is used to preserve the biological structure of tissue samples harvested from biopsy as closely as possible to their in vivo state for the diagnoses and study of disease on the cellular level. This process includes fixation, dehydration, clearing, paraffin infiltration. The protocol follows paraffin block embedding, microtome slicing, staining, and microscope slide studying. Read More

Acta Histochem 2022 May 24;124(4):151882. Epub 2022 Mar 24.

Biomedical Science Post Graduate Program and Department of Structural, Genetic and Molecular Biology - DEBIOGEM - University of Ponta Grossa, Carlos Cavalcanti Avenue, 4748 Campus of Uvaranas, CEP 84030-900 Paraná, Brazil. Electronic address:

The subcutaneous rat implanted model is a preclinical approach used in studies to characterize the histocompatibility of materials that could be used as biomaterials. Biomaterials are obtained synthetically or from the environment, and they can be used to treat or replace any tissues or organs that the body has lost. To execute their roles, the biomaterials must present any level of histocompatibility and a lower level of inflammatory reaction. Read More