2,008 results match your criteria Acta Cirurgica Brasileira[Journal]


Changes of renal histopathology and the role of Nrf2/HO-1 in asphyxial cardiac arrest model in rats.

Acta Cir Bras 2021 19;36(6):e360607. Epub 2021 Jul 19.

Full Professor. College of Veterinary Medicine and Bio-safety Research Institute - Jeonbuk National University - Iksan, South Korea.

Purpose: To investigate the role of Nrf2/HO-1 in renal histopathological ailments time-dependently in asphyxial cardiac arrest (CA) rat model.

Methods: Eighty-eight Sprague Dawley male rats were divided into five groups of eight rats each. Asphyxial CA was induced in all the experimental rats except for the sham group. Read More

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Comparison of two different biomaterials in the bone regeneration (15, 30 and 60 days) of critical defects in rats.

Acta Cir Bras 2021 19;36(6):e360605. Epub 2021 Jul 19.

Associate Professor. Department of Morphology and Pathology - Universidade Federal de São Carlos - Sao Carlos (SP), Brazil.

Purpose: To evaluate and compare two types of different scaffolds in critical bone defects in rats.

Methods: Seventy male Wistar rats (280 ± 20 grams) divided into three groups: control group (CG), untreated animals; biomaterial group 1 (BG1), animals that received the scaffold implanted hydroxyapatite (HA)/poly(lactic-co-glycolic) acid (PLGA); and biomaterial group 2 (BG2), animals that received the scaffolds HA/PLGA/Bleed. The critical bone defect was induced in the medial region of the skull calotte with the aid of an 8-mm-diameter trephine drill. Read More

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Platelet-concentrated and platelet poor-plasma promote different pattern on immunohistochemical expression of TGF-β1, however they impairs the osteoneogensis in calvarial defects treated with autograft due suppression of osteocalcin.

Acta Cir Bras 2021 19;36(6):e360604. Epub 2021 Jul 19.

PhD. Medical Research Institute - Hospital Universitário Evangélico Mackenzie - Curitiba (PR), Brazil.

Purpose: Herein we evaluated the effects of platelet concentrate (PC) and platelet-poor plasma (PPP) on bone repair using noncritical defects in the calvaria of rabbits and compared them to the presence of TGF-β1 and osteocalcin on reparative sites.

Methods: Five noncritical defects of 8.7 mm in diameter were created on the calvaria of 15 animals. Read More

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Cervical ventral slot in rabbits (Oryctolagus cuniculus). Piezosurgery versus conventional technique.

Acta Cir Bras 2021 9;36(6):e360606. Epub 2021 Jul 9.

DVM, PhD. Department of Veterinary Medicine and Surgery - College of Agricultural Sciences and Veterinary Medicine - Universidade Estadual Paulista "Julio de Mesquita Filho" - Jaboticabal (SP), Brazil.

Purpose: To investigate the applicability of piezosurgery for cervical ventral slot (CVS), comparing it with the conventional technique of using high-speed burs for bone wear.

Methods: Thirty rabbits (Oryctolagus cuniculus) were divided into two treatment groups (T1 and T2) corresponding to CVS between C3-C4. In T1, the surgery was performed with piezoelectric apparatus, and in T2 with high-speed burs. Read More

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Effect of S53P4 bioactive glass and low-level laser therapy on calvarial bone repair in rats submitted to zoledronic acid therapy.

Acta Cir Bras 2021 9;36(6):e360603. Epub 2021 Jul 9.

PhD, Assistant Professor. Oral and Maxillofacial Surgery - Department of Health Sciences - Dental School - Universidade de Araraquara - Araraquara (SP), Brazil.

Purpose: To evaluate the influence of bioactive glass and photobiomodulation therapy (PBMT) in calvarial bone repair process in rats submitted to zoledronic acid therapy.

Methods: Twenty-four rats were selected and treated with the dose of 0.035 mg/kg of zoledronic acid every two weeks, totalizing eight weeks, to induce osteonecrosis. Read More

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Sentinel lymph node biopsy using single-agent mapping tracer (blue dye) after neoadjuvant chemotherapy in a Brazilian cohort of breast cancer patients. Real world evidence.

Acta Cir Bras 2021 2;36(6):e360608. Epub 2021 Jul 2.

Graduate student. School of Medicine - Centro Universitário de Volta Redonda (Unifoa) - Volta Redonda (RJ), Brazil.

Purpose: To reduce false-negative rates (FNR) in sentinel lymph node biopsy (SLNB) of clinically positive (cN+) axilla in patients undergoing neoadjuvant chemotherapy (NAC). The removal of three or more lymph nodes with dual-tracer mapping including a radioisotope was used. However, in the Brazilian Unified Health System, the radioisotope tracer is not feasible in some hospitals. Read More

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Thoracic aortic aneurysm. An experimental model in pigs.

Acta Cir Bras 2021 25;36(6):e360602. Epub 2021 Jun 25.

Associate Professor. Medicine Faculty, Universidade Federal do Rio Grande do Sul. Head. Vascular Surgery Department - Hospital de Clínicas de Porto Alegre - Porto Alegre (RS), Brazil.

Purpose: To describe an unpublished experimental model of descending thoracic aortic aneurysm in pigs.

Methods: Ten Landrace female pigs aged 10 to 12 weeks old and with initial weights from 17 to 25 kg were anesthetized and their descending thoracic aortas exposed by fifth intercostal space left thoracotomy. The thoracic aorta was isolated. Read More

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Locking compression plate fixation of critical-sized bone defects in sheep. Development of a model for veterinary bone tissue engineering.

Acta Cir Bras 2021 25;36(6):e360601. Epub 2021 Jun 25.

PhD. Associate Professor. Department of Surgery - School of Veterinary Medicine and Animal Science - Universidade de São Paulo - Sao Paulo (SP), Brazil.

Purpose: To develop a segmental tibial bone defect model for tissue engineering studies in veterinary orthopedics using single locking compression plate (LCP) fixation and cast immobilization.

Methods: A 3-cm bone defect was created in the right tibia of 18 adult Suffolk sheep. A 10-hole, 4. Read More

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Use of hypertonic glucose (10%) in the prevention of postoperative adhesions in rats.

Acta Cir Bras 2021 25;36(5):e360504. Epub 2021 Jun 25.

PhD. Universidade Federal do Maranhão - College of Medicine - Department of Medicine I - São Luís (MA), Brazil.

Purpose: To evaluate the efficacy of hypertonic glucose (10%), alone or in combination with the corticoid dexamethasone, to prevent peritoneal adhesion following hysterectomy in rats.

Methods: Forty-two adult rats underwent hysterectomy with peritoneal lavage: G1 - glucose (10%); G2 - glucose (10%) and dexamethasone 3 mg·kg-1; and G3 - physiological saline (PS) 0.9%. Read More

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Development and validation of a simulator for teaching minimally invasive thoracic surgery in Brazil.

Acta Cir Bras 2021 18;36(5):e360508. Epub 2021 Jun 18.

Assistant Professor. Department of Surgery - Centro Universitário Unichristus - Fortaleza (CE), Brazil.

Purpose: To develop and validate a chest cavity simulator for teaching video-assited thoracic surgery (VATS).

Methods: The first phase of the study consisted of developing a chest cavity simulator. A quasi-experimental study was performed in the second phase, and 25 surgeons and residents participated in a three-stage pulmonary suture experiment. Read More

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Effects of nandrolone decanoate on femur morphology. Experimental study.

Acta Cir Bras 2021 18;36(5):e360507. Epub 2021 Jun 18.

PhD. Laboratory for Research on Translational Histomorphometry - Nature Science Department - Universidade Federal Fluminense - Rio das Ostras (RJ), Brazil.

Purpose: To evaluate the immediate and late effects of nandrolone on femur morphology of rats.

Methods: Twenty-eight animals with 20 weeks of age were divided into four groups: C28, control animals that were euthanized eight weeks after the experiment started; C40, control animals euthanized 20 weeks after the experiment started; T28, treated animals receiving nandrolone during eight weeks and euthanized immediately after the treatment period; and T40, animals treated during eight weeks and euthanized 12 weeks after the end of the treatment. Treated animals received nandrolone decanoate during eight weeks and control groups received peanut oil by intramuscular injection. Read More

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Biomechanical evaluation of a new femoral stem design for total hip replacement in a canine model.

Acta Cir Bras 2021 18;36(5):e360506. Epub 2021 Jun 18.

Fellow PhD degree. Postgraduate Program in Veterinary - Universidade Estadual Paulista "Julio de Mesquita Filho" - Jaboticabal (SP), Brazil.

Purpose: To evaluate the biomechanical properties of a novel total hip replacement femoral stem.

Methods: Eight pairs of femurs from dog cadavers were used. The femurs were separated into different groups. Read More

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Techniques of orthotopic renal transplantation. II. Size-matched porcine grafts in monkey recipients.

Acta Cir Bras 2021 18;36(5):e360503. Epub 2021 Jun 18.

PhD. Department of Kidney Regenerative Medicine - The Jikei University School of Medicine - Tokyo, Japan.

Purpose: As a classical xenotransplantation model, porcine kidneys have been transplanted into the lower abdomen of non-human primates. However, we have improved upon this model by using size-matched grafting in the orthotopic position. The beneficial aspects and surgical details of our method are reported herein. Read More

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Histological evaluation of capsules formed by texturized silicone implants with and without polyester mesh coverage (Parietex®). A study on female rats.

Acta Cir Bras 2021 14;36(5):e360505. Epub 2021 Jun 14.

PhD. School of Medicine - Pontifícia Universidade Católica do Paraná - Curitiba (PR), Brazil.

Purpose: To evaluate capsules formed by microtextured silicone implants with and without Parietex® mesh coverage histologically.

Methods: Sixty Wistar rats were divided in two groups (meshed and unmeshed). Each group was, then, divided into two subgroups for evaluation at 30 and 90 days. Read More

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Evaluation of barbed suture for celiorrhaphy and subcutaneous closure in bitches with pyometra submitted to ovariohysterectomy.

Acta Cir Bras 2021 14;36(5):e360502. Epub 2021 Jun 14.

Resident. School of Veterinary Medicine - Universidade Federal de Mato Grosso - Cuiaba (MT), Brazil.

Purpose: To evaluate the use of barbed sutures over the surgical time, the leukogram, the tissue thickness in which the sutures were employed (ultrasonography), the costs, and the possible complications in bitches with pyometra submitted to ovariohysterectomy (OH).

Methods: Convectional 2.0 polyglyconate suture was used in the control group (CG n = 10) and 2. Read More

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Effect of IL-33 on pyroptosis of macrophages in mice with sepsis via NF-κB/p38 MAPK signaling pathway.

Acta Cir Bras 2021 14;36(5):e360501. Epub 2021 Jun 14.

PhD. The 2nd Clinical Medical College - Zhejiang Chinese Medical University - Hangzhou - Zhejiang, China.

Purpose: To demonstrate the effect of IL-33 on the macrophage pyroptosis in mice with sepsis through the NF-kB/p38 MAPK signal pathway.

Methods: In total, 24 C57BL/6 mice were divided into the sham operation group (sham) and the cecal ligation and puncture group (CLP). After CLP, 24 IL-33-/- mice were divided into the IL-33-/- group and the IL-33-/- intervention group. Read More

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Controlled decompression alleviates early brain injury in rabbit intracranial hypertension model by regulating apoptosis/necroptosis.

Acta Cir Bras 2021 28;36(4):e360406. Epub 2021 May 28.

MD, PhD. Department of Neurosurgery - Wuxi Medical College of Anhui Medical University - 904th Hospital of PLA (101th Hospital of PLA) - Wuxi, China.

Purpose: To evaluate the effects of controlled decompression and rapid decompression, explore the potential mechanism, provide the theoretical basis for the clinical application, and explore the new cell death method in intracranial hypertension.

Methods: Acute intracranial hypertension was triggered in rabbits by epidural balloon compression. New Zealand white rabbits were randomly put into the sham group, the controlled decompression group, and the rapid decompression group. Read More

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Shen-fu injection alleviates acute renal injury by reducing cytokine levels and modulating apoptosis in a porcine hemorrhagic shock model.

Acta Cir Bras 2021 28;36(4):e360405. Epub 2021 May 28.

PhD. Capital Medical University - Beijing ChaoYang Hospital - Department of Emergency - Beijing, China.

Purpose: Shen-fu injection (SFI) was used to intervene in the resuscitation of porcine hemorrhagic shock (HS) model to study its protective effects on acute kidney injury.

Methods: After 60 min of HS, 28 animals were randomly assigned into four groups. The groups were as follows: hemorrhagic shock group (HS); HS resuscitation with shed-blood group (HSR); HS resuscitation with shed-blood and SFI (1 mL·kg-1) group (HSR-SFI); and the sham operation group (Sham). Read More

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Beneficial effects of octreotide in alcohol-induced neuropathic pain. Role of H 2S, BDNF, TNF-α and Nrf2.

Acta Cir Bras 2021 31;36(4):e360408. Epub 2021 May 31.

MM. Ninth Hospital of Xi'an - Intensive Care Unit - Shaanxi, China.

Purpose: To explore the role and molecular mechanisms of neuroprotective effects of octreotide in alcohol-induced neuropathic pain.

Methods: Male Wistar rats were employed and were administered a chronic ethanol diet containing 5% v/v alcohol for 28 days. The development of neuropathic pain was assessed using von Frey hair (mechanical allodynia), pinprick (mechanical hyperalgesia) and cold acetone drop tests (cold allodynia). Read More

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Fibrin biopolymer sealant and aquatic exercise association for calcaneal tendon repair.

Acta Cir Bras 2021 21;36(4):e360407. Epub 2021 May 21.

PhD. Universidade Brasil - Scientific and Technological Institute - Sao Paulo (SP), Brazil.

Purpose: The aim of this work was to analyze the effect of fibrin biopolymer sealant (FS) associated or not to aquatic exercise (AE) on the calcaneal tendon repair.

Methods: Forty-four female Wistar rats were randomly divided into four experimental groups: Lesion control (L), Lesion and FS (LS), Lesion and AE (LE) and Lesion and FS associated to AE (LSE). The edema volume (EV), collagen ratio, and histopathological analysis were evaluated after 7, 14, and 21 days of partial tendon transection. Read More

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Three-dimensional printed poly (L-lactide) and hydroxyapatite composite for reconstruction of critical bone defect in rabbits.

Acta Cir Bras 2021 21;36(4):e360404. Epub 2021 May 21.

Assistant Professor. Universidade Estadual Paulista "Júlio de Mesquita Filho" - Faculty of Agrarian and Veterinary Sciences - Department of Clinical and Veterinary Surgery - Jaboticabal (SP), Brazil.

Purpose: To use a 3D printed poly (L-lactide) acid (PLLA) and hydroxyapatite (HA) composite as a bone substitute for reconstruction of a critical bone defect in the radius of rabbits.

Methods: A 1.5 cm ostectomy was performed in the radial diaphysis of 60 New Zealand white rabbits. Read More

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Effects of Rehmannia glutinosa polysaccharides on bone tissue structure and skeletal muscle atrophy in rats with disuse.

Acta Cir Bras 2021 14;36(4):e360403. Epub 2021 May 14.

PhD, Associate Professor. Shaanxi University of Chinese Medicine - College of Pharmacy - Department of Clinical Chinese Pharmacy - Xianyang, China.

Purpose: To study effects of Rehmannia glutinosa polysaccharides (RGP) on bone tissue structure and skeletal muscle atrophy in rats with disuse.

Methods: A rat model of disuse osteoporosis combined with muscle atrophy was established by removing the bilateral ovaries of rats and fixing their hind limbs for a long time. Forty SD rats were administered intragastrically for 12 weeks. Read More

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Tissue content of metalloproteinase-9 and collagen in the colon with and without fecal stream after intervention with infliximab in rats subjected to Hartmann's surgery.

Acta Cir Bras 2021 14;36(4):e360401. Epub 2021 May 14.

PhD, Associate Professor. Universidade São Francisco - Faculty of Medicine - Postgraduate Program in Health Sciences - Bragança Paulista (SP), Brazil.

Purpose: Quantify the tissue content of metalloproteinase-9 (MMP-9) and collagen in colic mucosa with and without intestinal transit after infliximab administration in rats subjected to Hartmann's surgery.

Methods: Twenty-two rats underwent colon diversion by Hartmann's surgery. Animals were maintained with intestinal bypass for 12 weeks to induce development of diversion colitis (DC). Read More

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Cyclosporine reduces the spleen dimensions in rabbits.

Acta Cir Bras 2021 7;36(4):e360402. Epub 2021 May 7.

Full Professor. Universidade Federal de Minas Gerais - Medical School - Department of Surgery - Belo Horizonte (MG), Brazil.

Purpose: To assess the influence of prolonged cyclosporine use on the macro- and microscopic morphology of the spleen.

Methods: 16 adult rabbits were divided into two groups (n = 8): group 1 - a placebo group, which was followed-up over a period of nine months; group 2 - which had taken an oral dose of cyclosporine (10 mg·kg-1·day-1) over nine months. At the end of this period, the splenic histoarchitecture of all animals was evaluated and the splenic corpuscles were measured. Read More

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Ovarian pedicle hemostasis techniques in cats.

Acta Cir Bras 2021 7;36(3):e360308. Epub 2021 May 7.

Full Professor. Universidade Federal do Piauí - Department of Clinical and Veterinary Surgery - Veterinary Surgical Clinic - Teresina (PI), Brazil.

Purpose: To evaluate hemostasis of the ovarian arteriovenous complex (OAVC) in relation to surgical time, practicality and feasibility in three ovariohysterectomy (OH) techniques for queens.

Methods: The experiment was performed on 21 female cats aged between six months and seven years, randomly arranged into three groups in a completely randomized design. Group one was spayed using the conventional three-clamp technique, group two using the OAVC knotting technique, and group three using the ovarian pedicle rotation technique. Read More

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The effect of mild hypothermia plus rutin on the treatment of spinal cord injury and inflammatory factors by repressing TGF-β/smad pathway.

Acta Cir Bras 2021 7;36(3):e360307. Epub 2021 May 7.

PhD. Department of Spine Surgery - Guizhou Orthopedic Hospital - Guizhou Province, China.

Purpose: To probe the mechanism of mild hypothermia combined with rutin in the treatment of spinal cord injury (SCI).

Methods: Thirty rats were randomized into the following groups: control, sham, model, mild hypothermia (MH), and mild hypothermia plus rutin (MH+Rutin). We used modified Allen's method to injure the spinal cord (T10) in rats, and then treated it with MH or/and rutin immediately. Read More

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Cardioprotection stimulated by resveratrol and grape products prevents lethal cardiac arrhythmias in an animal model of ischemia and reperfusion.

Acta Cir Bras 2021 7;36(3):e360306. Epub 2021 May 7.

Associate Professor. Universidade Federal de São Paulo - Department of Pharmacology - São Paulo (SP), Brazil.

Purpose: To evaluate the preventive cardioprotective effects of resveratrol and grape products, such as grape juice and red wine, in animal model of cardiac ischemia and reperfusion.

Methods: Male Wistar rats orally pretreated for 21-days with resveratrol and grape products were anesthetized and placed on mechanical ventilation to surgically induce cardiac ischemia and reperfusion by obstruction (ischemia) followed by liberation (reperfusion) of blood circulation in left descending coronary artery. These rats were submitted to the electrocardiogram (ECG) analysis to evaluate the effects of pretreatment with resveratrol and grape products on the incidence of ventricular arrhythmias (VA), atrioventricular block (AVB) and lethality (LET) resulting from cardiac ischemia and reperfusion. Read More

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Effects of the lower energy and pulse stacking in carbon dioxide laser skin treatment: an objective analysis using second harmonic generation.

Acta Cir Bras 2021 7;36(3):e360304. Epub 2021 May 7.

Associate Professor, Head. Universidade Estadual de Campinas - Faculty of Medical Sciences - Department of Surgery - Campinas (SP), Brazil.

Purpose: To evaluate the effect of fractional carbon dioxide (CO2) laser treatment using lower power associated with pulse stacking within collagen fibers, using second harmonic generation microscopy and computerized image analysis.

Methods: Twenty male Wistar rats aging eight weeks were used. Each treatment area received a single-pass CO2 fractional laser with different parameters. Read More

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Effects of dietary creatine supplementation on kidney and striated skeletal muscles of rats submitted to ischemia and reperfusion of hind limbs.

Acta Cir Bras 2021 21;36(3):e360305. Epub 2021 Apr 21.

PhD, Assistant Professor. Universidade de Mogi das Cruzes - College of Medicine - Department of Pathology - Mogi das Cruzes (SP), Brazil.

Purpose: To evaluate the effect of creatine supplementation in the diet of rats subjected to ischemia and reperfusion of hind limbs.

Methods: Eighteen male Wistar rats were randomized to receive dietary creatine supplementation (G1) or no supplementation (G2), before being subjected to 4 h of ischemia followed by 4 h of reperfusion. In addition, 10 rats (G3) underwent the same surgical procedure, without ischemia, but with supplementation. Read More

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Carboxymethyl chitosan hydrogel formulations enhance the healing process in experimental partial-thickness (second-degree) burn wound healing.

Acta Cir Bras 2021 5;36(3):e360303. Epub 2021 Apr 5.

PhD. Universidade Federal de Goiás - Instituto de Patologia Tropical e Saúde Pública - Departamento de Biociências e Tecnologia - Goiânia (GO), Brazil.

Purpose: This study aimed to elaborate a hydrogel constituted by carboxymethyl chitosan (CMC), hyaluronic acid (HA) and silver (Ag) and to evaluate its healing effect on partial-thickness burn wounds experimentally induced in rats.

Methods: CMC was obtained by chitosan reacting with monochloroacetic acid. The carboxymethylation was confirmed by Fourier-transform infrared spectroscopy and hydrogen nuclear magnetic resonance (NMR). Read More

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