4,376 results match your criteria Acta Biochimica Polonica[Journal]

Cucurbitacin E reduces the cognitive dysfunction induced by sevoflurane in rats by regulating NF-κB pathway.

Acta Biochim Pol 2022 Jun;69(2):387-391

Department of Anesthesiology, Union hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan430022, China.

Background: Perioperative neurocognitive disorders (PND) occur frequently and refer to alterations in cognitive function after surgery, especially in elderly patients. PND is characterized as abnormalities of learning, memory, language, and emotions. Cucurbitacin E has been reported to possess various pharmacological properties, including anticancer, antiviral, and anti-inflammatory effects. Read More

View Article and Full-Text PDF

Combination of metformin and oxaliplatin inhibits gastric cancer cell proliferation and induces apoptosis.

Acta Biochim Pol 2022 Jun;69(2):321-326

Department of General Surgery, Wuhan No.4 Hospital, Wuhan, 430033, China.

Background: Gastric cancer is one of the most common cancers worldwide. The disease has a poor prognosis, especially when the tumor becomes inoperable. The present study investigated the potential synergistic effects of oxaliplatin and metformin in gastric cancer cells. Read More

View Article and Full-Text PDF

USP18 contributes to the proliferation and migration of ovarian cancer cells by regulating the AKT/mTOR signaling pathway.

Acta Biochim Pol 2022 Jun;69(2):417-422

Tumor Ward 4, The 901ST Hospital of the Joint Logistics Support Force of PLA, Hefei, Anhui Province, China.

Ubiquitin-specific peptidase (USP)18 is elevated in tumor tissues and is associated with tumor malignancy. USP18 functions as an oncogene in different cancers. However, the role of USP18 in ovarian cancer was poorly understood. Read More

View Article and Full-Text PDF

Glycitein exerts neuroprotective effects in Rotenone-triggered oxidative stress and apoptotic cell death in the cellular model of Parkinson's disease.

Acta Biochim Pol 2022 Jun;69(2):447-452

Department of Neurology, The Third Affiliated Hospital of Soochow University, Changzhou, 213003, China.

Parkinson's disease (PD) is one of the prevalent neurodegenerative diseases among aging populations. Despite recent advancements, still, no therapy exists for the prevention of PD. Given the remarkable pharmacological properties of flavonoids, this study was designed to investigate the protective effects of a plant-derived flavonoid, glycitein in a cellular model of PD. Read More

View Article and Full-Text PDF

Serum miR-21 predicts the prognosis of patients with primary gastrointestinal diffuse large B-cell lymphoma.

Acta Biochim Pol 2022 Jun;69(2):379-385

Department of Pathology, the Affiliated Hospital of Zunyi Medical University, Zunyi, China.

Background: Primary gastrointestinal diffuse large B-cell lymphoma (PGI-DLBCL) lacks specific clinical manifestations and its malignancy renders prognostication and choice of treatment strategy difficult. The aim of this study was to evaluate microRNA (miR)-21 as potential non-invasive biomarkers for prognosis in PGI-DLBCL patients.

Methods: Serum miR-21 expression in de novo PGI-DLBCL patients, consecutively enrolled for this study, was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Read More

View Article and Full-Text PDF

Anticancer effects of zapotin flavone in human gastric carcinoma cells are mediated via targeting m-TOR/PI3K/AKT signalling pathway.

Acta Biochim Pol 2022 Jun;69(2):465-469

Department of Pharmacy, Shaanxi Provincial Cancer Hospital, Xi'an, Shaanxi, China.

The current study aims to investigate the anticancer effects of zapotin flavone in human gastric carcinoma cells. MTT assay was performed to determine the cytotoxicity effects of zapotin against the gastric cancer cells (SNU-1) and normal gastric cells (GES-1). SNU-1 cell morphology was analyzed through phase-contrast microscopy. Read More

View Article and Full-Text PDF

Identification of significant prognostic risk markers for pancreatic ductal adenocarcinoma: a bioinformatic analysis.

Acta Biochim Pol 2022 Jun;69(2):327-333

Department of Pancreatobiliary Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.

Objective: This study aimed to identify novel prognostic biomarkers of pancreatic ductal adenocarcinoma (PDAC) using bioinformatics analyzes.

Methods: Clinical information, microRNAs (miRNAs), and genes expression profile data from PDAC cases were downloaded from the Cancer Genome Atlas (TCGA) database. The potential prognostic risk miRNAs and genes were screened using the Elastic Net Cox proportional risk regression hazards (EN-COX) model. Read More

View Article and Full-Text PDF

Isoalantolactone exerts anticancer effects on human HEC-1-B endometrial cancer cells via induction of ROS mediated apoptosis and inhibition of MEK/ERK signalling pathway.

Fang Hu Ping Yang

Acta Biochim Pol 2022 Jun;69(2):453-458

Department of Reproductive Medicine, Maternal and Child Health Hospital of Hubei Province, Wuhan 430070,Hubei,China.

Isoalantolactone has been shown to inhibit the growth of different cancer cells. The objective of the present study was to evaluate the effects of isoalantolactone on the proliferation of endometrial cancer HEC-1-B cells. Results showed that isoalantolactone suppressed the proliferation of HEC-1-B cells in a concentration-dependent manner and exhibited an IC50 of 10 µM. Read More

View Article and Full-Text PDF

MiR-132-3p inhibits proliferation, invasion and migration of colorectal cancer cells via down-regulating FOXP2 expression.

Acta Biochim Pol 2022 Jun;69(2):371-377

Department of Surgery, Guangdong Second Provincial General Hospital, Guangzhou, China.

Objective: colorectal cancer (CRC) is a common cancer with high mortality. This study aimed to investigate the role of microRNA (miR)-132-3p on proliferation, invasion and migration of CRC cells.

Materials And Methods: qRT-PCR and Western blot analyses were used to determine the expression of miR-132-3p and forking box (FOX) protein 2 (FOXP2) in CRC cell line CACO-2. Read More

View Article and Full-Text PDF

U6/miR-211 expression ratio as a purity parameter for HEK293 cell-derived exosomes.

Acta Biochim Pol 2022 May;69(2):409-415

Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Łódź, Poland.

Small extracellular vesicles (sEVs) including exosomes are produced by all cell types and can be isolated from biological fluids and cell culture supernatants. The separation of exosomes with high purity from protein-rich media remains challenging. Besides contaminating proteins, small microvesicles (MVs) and apoptotic bodies are usually co-isolated with exosomes. Read More

View Article and Full-Text PDF

Use of dissected paraffin block tissue as a source of mRNA for transcriptional profiling and biomarker identification: a review, with preliminary findings in adrenocortical carcinoma tissue.

Acta Biochim Pol 2022 May;69(2):273-281

King's College Hospital, London, SE5 9RS UK.

Cancer tissue obtained by biopsy or at surgery is usually handled and stored as formalin-fixed, paraffin-embedded (FFPE) blocks. While primarily prepared for histological examination, this material represents a valuable resource that is stable and transportable, and which is ideal for examination in 3 dimensions. It has been used for DNA and protein analysis but the potential for transcriptional profiling has been little exploited: the quality of the extracted RNA is uncertain and there are few reports of its efficient use. Read More

View Article and Full-Text PDF

The MiR-101/EZH2 negative feedback signaling drives oxygen-glucose deprivation/reperfusion-induced injury by activating the MAPK14 signaling pathway in SH-SY5Y cells.

Acta Biochim Pol 2022 May;69(2):437-446

Department of Anesthesiology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huai'an City, Jiangsu Province, 223300, China.

MiR-101 has been reported to be involved in neuroinflammation, neuronal injury and neurotoxicity. However, the specific role and mechanism of miR-101 in ischemia/reperfusion (I/R)-induced neuronal injury remain largely unknown. Our study found that after oxygen-glucose deprivation/reperfusion (OGD/R) exposure, the level of miR-101 in SH-SY5Y cells was significantly decreased, which was accompanied by a decrease in cell viability, and an increase in LDH release and apoptosis. Read More

View Article and Full-Text PDF

Krüppel-like factor 4 promotes the proliferation and osteogenic differentiation of BMSCs through SOX2/IGF2 pathway.

Acta Biochim Pol 2022 May;69(2):349-355

Department of Orthopaedics, Funing People's Hospital, Yancheng City, Jiangsu Province, China.

Objectives: Human bone marrow mesenchymal stem cells (BMSCs) have multi-lineage differentiation potential and have been widely researched in regenerative medicine. The purpose of this research was to explore whether Krüppel-like factor 4 (KLF4) can regulate the osteogenic differentiation of BMSCs.

Methods: We transfected human BMSCs with KLF4 overexpression plasmid and si-KLF4 to study the effects of KLF4. Read More

View Article and Full-Text PDF

Saprirearine protects H9c2 cardiomyocytes against hypoxia/reoxygenation-induced apoptosis by activating Nrf2.

Acta Biochim Pol 2022 May;69(2):429-436

Department of Cardiology, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huaian, Jiangsu 223300, China.

Myocardial infarction is a major cause of mortality and disability worldwide. Ischemia/reperfusion injury is the key factor that results in the increase in infarct size in pathogenesis. To find a novel therapy for myocardial infarction, we have evaluated saprirearine, a natural diterpenoid, using H9c2 cardiomyocytes injured by hypoxia/reoxygenation and explored the possible mechanisms. Read More

View Article and Full-Text PDF

Brinzolamide- and latanoprost-loaded nano lipid carrier prevents synergistic retinal damage in glaucoma.

Acta Biochim Pol 2022 May;69(2):423-428

Fundamentals of Integrated Traditional Chinese and Western Medicine, School of Basic Medical Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

Glaucoma is a common eye disease and a major cause of blindness. We designed brinzolamide (Brla)- and latanoprost (Ltp)-loaded nano-lipoidal carriers (NLCs) for glaucoma treatment. Brla and Ltp-loaded NLCs were designed and characterized by assessing the zeta potential, polydispersity index, X-ray diffraction and scanning electron microscopy images, and particle size. Read More

View Article and Full-Text PDF

Proteomics analysis of lipid droplets in mouse neuroblastoma cells.

Acta Biochim Pol 2022 May;69(2):399-407

School of Life and Pharmaceutical Sciences, Dalian University of Technology, Panjin, China.

Lipid droplets (LDs) are intracellular droplets containing phospholipids and neutral lipids. It is well known that LDs are organelles with a rich proteome. In the nervous system, these droplets may play an important role in maintaining the normal physiological function of nerve cells. Read More

View Article and Full-Text PDF

miR-342-3p Suppresses glioblastoma development via targeting CDK6.

Acta Biochim Pol 2022 May;69(2):393-397

Department of Pediatric Surgery, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou 362000, Fujian, China.

Background: Glioblastoma is the most malignant primary brain tumor with dysregulated microRNAs affecting development and malignant transformation.

Methods: Gene Expression Omnibus (GEO) dataset (GSE165937) was retrieved, and the differential expressed microRNAs were screened and testified by quantitative real-time PCR (qRT-PCR) in glioblastoma cells. miR-342-3p mimic was transfected into U87MG and U251MG cells. Read More

View Article and Full-Text PDF

MicroRNA-145 regulates the proliferation of the human gastric cancer cells by targeting tuftelin 1 (TUFT1).

Acta Biochim Pol 2022 May;69(2):357-362

Department of General Surgery, Wusong Central Hospital, Baoshan District, Shanghai, 200940, China.

MicroRNA-145 (miR-145) has been shown to regulate the development of different human cancer. However, the role of miR-145 via modulation of tuftelin 1 (TUFT1) expression has not been studied in gastric cancer. TUFT1The results showed that gastric cancer tissues and cell lines exhibit significant (P<0. Read More

View Article and Full-Text PDF

miR-3942-3p Increases the radiosensitivity of nasopharyngeal carcinoma through negatively regulating BARD1.

Acta Biochim Pol 2022 May;69(2):335-341

Department of Radiotherapy, Center of Oncology, People's Hospital of Guangxi Zhuang Autonomous Region, China.

Nasopharyngeal carcinoma (NPC) has high incidence in China and East and Southeast Asia. The study was performed to investigate the effect of microRNA3942-3p (miR-3942-3p) on the radiosensitivity of NPC. Compared with non-cancer tissue, NPC had significantly lower miR-3942-3p expression. Read More

View Article and Full-Text PDF

Circ_0014359 promotes oral squamous cell carcinoma progression by sponging miR-149.

Acta Biochim Pol 2022 May;69(2):315-320

Department of Stomatology, Hunan Province People's Hospital, Changsha, Hunan, China.

This study aimed to explore the role and mechanism of circ_0014359 in the OSCC. We firstly investigated the expression levels of circ_0014359 in OSCC tissues and cell lines. Then, the effects of knocking down circ_0014359 on cellular viability, apoptosis, migration, and invasion of OSCC cell lines were observed by cell counting kit-8 assay, flow cytometry, and transwell assay. Read More

View Article and Full-Text PDF

Over-expression of EPB41L3 promotes apoptosis of human cervical carcinoma cells through PI3K/AKT signaling.

Acta Biochim Pol 2022 May;69(2):283-289

Fifth Department of Gynecologic Surgery, Xinjiang Medical University Affiliated Tumor Hospital, Urumqi, China.

Background: Cervical cancer is a significant malignancy of the female reproductive system. This study aimed to investigate the functions of Erythrocyte Membrane Protein Band 4.1 Like 3 (EPB41L3) in cervical cancer and to explore its underlying mechanisms. Read More

View Article and Full-Text PDF

Hydrogen peroxide is formed upon cooking of vegetables.

Acta Biochim Pol 2022 May;69(2):471-474

Laboratory of Analytical Biochemistry, Institute of Food Technology and Nutrition, College of Natural Sciences, Rzeszow University, Rzeszow, Poland.

Hydrogen peroxide (H2O2) is generated under autoxidation of some components of beverages including flavonoids and ascorbate, especially in tea and coffee. As polyphenols are also present in solid food, especially in vegetables, we checked whether hydrogen peroxide is generated during cooking of several common vegetables. The formation of hydrogen peroxide was found in the decoctions of all cooked vegetables studied except for potato and in the homogenates of cooked vegetables except for garlic and purple potato. Read More

View Article and Full-Text PDF

Preventive effect of salicin ether against type-2 diabetes mellitus through targeting PPARγ-regulated gene expression.

Acta Biochim Pol 2022 Apr 28. Epub 2022 Apr 28.

Department of Endocrinology, General Hospital of Central Theater Command of the People's Liberation Army, Wuhan, Hubei Province, 430070, P. R. China.

Diabetes mellitus is the syndrome associated with metabolism having complicated pathogenesis and its morbidity rate is rapidly increasing every year. The present study investigated the preventive effect of salicin ether against type-2 diabetes and explored the underlying mechanism. Salicin ether reduced PPARγ-LBD level and transcriptional property of RXRα-PPARγ in 293T cells. Read More

View Article and Full-Text PDF

6-Shogaol alleviates CCl4-induced liver fibrosis by attenuating inflammatory response in mice through the NF-κB pathway.

Acta Biochim Pol 2022 Apr;69(2):363-370

Henan University of Chinese Medicine, Zhengzhou, Henan, China.

Liver fibrosis is a global health problem caused by a number of diseases related to liver damage. 6-Shogaol is a biologically active substance derived from the rhizome of Zingiber officinale Roscoe with anti-tumor, anti-inflammatory, and antioxidant properties. To explore the effects of 6-Shogaol on liver fibrosis, we used a mouse model of the condition in which mice were injected intraperitoneally with carbon tetrachloride (CCl4) at a dose of 2 mL/kg three times per week for a period of 4 weeks. Read More

View Article and Full-Text PDF

LINC00313/miR-4429 axis provides novel biomarkers for the diagnosis and prognosis of non-small cell lung cancer.

Acta Biochim Pol 2022 Apr;69(2):343-348

Department of Radiotherapy, Affiliated Hospital of Weifang Medical University, Weifang 261031, Shandong, China.

Background: Increasing evidence suggests that the LncRNA/miRNA axis plays a key role in many types of tumorigenesis. However, the potential role and clinical value of LINC00313/miR-4429 in non-small cell lung cancer (NSCLC) remain elusive and need further study. The aim of this study was to investigate the role of LINC00313/miR-4429 axis in NSCLC. Read More

View Article and Full-Text PDF

The determination of haemagglutinin influenza antibodies in the Polish population in the epidemic season 2020/2021 during the SARS-CoV-2 pandemic.

Acta Biochim Pol 2022 Apr;69(2):459-464

Department of Influenza Research - National Influenza Center, National Institute of Public Health NIH - National Research Institute, Warsaw, Poland.

The aim of the study was to prove the level of antibodies against haemagglutinin in the sera of people from seven age groups in the epidemic season 2020/2021 in Poland to determine the differentiation of the antibody level and the protection rate depending on age. The level of anti-haemagglutinin antibodies was established by haemagglutinin inhibition test (HAI). A total of 700 randomly selected sera from people belonging to 7 different age groups were tested. Read More

View Article and Full-Text PDF

Anticancer activity of Germacrone terpenoid in human osteosarcoma cells is mediated via autophagy induction, cell cycle disruption, downregulating the cell cycle regulatory protein expressions and cell migration inhibition.

Acta Biochim Pol 2022 Apr;69(2):305-308

Department of Orthopaedic Trauma, Weifang People's Hospital, Weifang, Shandong, China.

Germacrone a sesquiterpene is a potential pharmacological agent with important medicinal applications. It is a potential anticancer agent and has been reported for anticancer activity against hepatoma cells and breast cancer cells, additionally, it has also shown anti-inflammatory, antioxidant, and antifungal activity. Therefore, this study was designed to testify anticancer activity of germacrone terpenoid in human osteosarcoma cells along with studying its effects of autophagy induction, cell cycle disruption, downregulating the cell cycle regulatory protein expressions and cell migration inhibition. Read More

View Article and Full-Text PDF

LINC00342 induces metastasis of lung adenocarcinoma by targeting miR-15b/TPBG.

Acta Biochim Pol 2022 Apr;69(2):291-297

Department of Cardiothoracic Surgery, Hebei Province Hospital of Chinese Medicine, Shijiazhuang, PR China.

In this study, the function and regulation of long non-coding RNA (lncRNA) LINC00342 in lung adenocarcinoma were investigated. From The Cancer Genome Atlas (TCGA) datasets and Gene Expression Omnibus (GEO) datasets, LINC00342 was found to be up-regulated in lung adenocarcinoma. The high expression of LINC00342 was also validated in lung cancer cell lines. Read More

View Article and Full-Text PDF

Effects of vitamin B12 supplementation on pain relief in certain diseases - a literature review.

Acta Biochim Pol 2022 Apr;69(2):265-271

Faculty of Medicine, Centre for Innovative Research in Medical and Natural Sciences, University of Rzeszów, Rzeszów Poland.

Based on current scientific reports, vitamin B12 (cobalamin), used in the treatment of pernicious anemia, may be used as a pain reliever. It has been shown to be effective in the treatment of different sorts of pain, including diabetic neuropathic, musculoskeletal, abdominal, back and spine ones, but also pain in Alzheimer's disease and pain ailments resulting from the development of neoplasms. In addition, it helps regeneration of the sensory nerves and relieves pain in eye diseases, for example in dry eye syndrome. Read More

View Article and Full-Text PDF

Antiproliferative effects of isoalantolactone in human liver cancer cells are mediated through caspase-dependent apoptosis, ROS generation, suppression of cell migration and invasion and targeting Ras/Raf/MEK signalling pathway.

Acta Biochim Pol 2022 Apr;69(2):299-304

Department of Pharmaceutics, Shijiazhuang People's Hospital, Shijiazhuang, Hebei 050000, China.

The main objective of this study was to evaluate the in vitro antiproliferative effects of isoalantolactone against liver cancer cells (Hep-G2) and also monitor its mechanism of action. The MTT assay was involved in proliferation assessments and phase contrast microscopy was used to check cellular morphology. Acridine orange/ethidium bromide staining along with western blotting was used to evaluate proapoptotic effects of isoalantolactone. Read More

View Article and Full-Text PDF