4,065 results match your criteria Acta Biochimica Polonica[Journal]


Iontophoretic delivery of transdermal patches containi.

Acta Biochim Pol 2019 Apr 15. Epub 2019 Apr 15.

Department of Otolaryngology-Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, Beijing Municipality, 100730, PR China.

The present clinical trial investigates the sustaining anesthetic effect of Ropivacaine when administered as epidural injection followed by transdermal patch with and without Reverse electrodialysis (RED) system in order to maintain anesthesia in children. It also focuses on rate of release of drug in-vitro when administered with RED system. The transdermal patch of Ropivacaine was prepared using evaporation technique enclosing 25 mg of Local anesthetic Ropivacaine. Read More

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http://dx.doi.org/10.18388/abp.2018_2669DOI Listing

The The influence of high doses of α-tocopherol on the content of selected trace elements in the liver of developing chicken embryos in experimentally induced 2,3,7,8-tetrachlorodibenzo-p-dioxin intoxication.

Acta Biochim Pol 2019 Apr 13. Epub 2019 Apr 13.

Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Wrocław, Poland.

A chicken embryo develops in ovo without access to a constant circulating maternal nutrient supply, and therefore all necessary nutrients are initially stored in the yolk, and with progressive development are transferred to the liver, where they are taken up in response to various needs. Fluctuations in hepatic trace elements correlate with their mobilization from egg stores. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) intoxication causes liver damage by production of free radicals, while α-tocopherol is a well-known antioxidant and may play a protective role. Read More

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http://dx.doi.org/10.18388/abp.2018_2789DOI Listing

Association of serum deiodinase type 2 level with chronic obstructive pulmonary disease in the Polish population.

Acta Biochim Pol 2019 Apr 12. Epub 2019 Apr 12.

Department of Pulmonology and Allergology, Medical University of Lodz, Łódź, Poland.

Backgrounds: Deiodinase type 2 (DIO2) is a selenoenzyme involved in the synthesis of thyroid hormones. Chemerin is a newly investigated adipokine known also as novel chemokine. Both molecules have been recently expected and found to play an important role in inflammation and immunity. Read More

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https://ojs.ptbioch.edu.pl/index.php/abp/article/view/2761
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http://dx.doi.org/10.18388/abp.2018_2761DOI Listing
April 2019
2 Reads

When size matters - coordination of growth and cell cycle in bacteria.

Acta Biochim Pol 2019 Apr 10. Epub 2019 Apr 10.

Department of Bacterial Molecular Genetics, University of Gdansk, Gdańsk, Poland.

Bacterial cells often inhabit environments where conditions can change rapidly. Therefore, a lot of bacterial species developed control strategies allowing them to grow and divide very fast during feast and slow down both parameters during famine. Under rich nutritional conditions, fast-growing bacteria can divide with time interval equal to half of the period required to synthesize their chromosomes. Read More

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https://ojs.ptbioch.edu.pl/index.php/abp/article/view/2798
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http://dx.doi.org/10.18388/abp.2018_2798DOI Listing
April 2019
1 Read

Evaluation of the localization and biological effects of PAMAM G3 dendrimer-biotin/pyridoxal conjugate as HaCaT keratinocyte targeted nanocarrier.

Acta Biochim Pol 2019 Apr 9. Epub 2019 Apr 9.

Department of Drug Technology and Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Warsaw, Poland.

Recognition of the molecular mechanisms of keratinocyte participation in normal skin homeostasis and in pathogenesis may lead to creation of more effective tools for topical application of cosmetics, cosmeceutics and drugs to a particular location within the skin for prevention and therapy of many skin disorders and diseases. For this purpose, the PAMAM G3 dendrimer with amide linkages of 9 biotin molecules and 10 molecules of pyridoxal phosphate (BC-PAMAM) was constructed, and its biological properties and cellular uptake and localization were investigated in the HaCaT keratinocytes. BC-PAMAM is nontoxic for HaCaT cells, as estimated by two assays (Neutral Red and tetrazolium salt reduction, XTT), and revealed low apoptosis induction at up to 50 µM concentration. Read More

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https://ojs.ptbioch.edu.pl/index.php/abp/article/view/2767
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http://dx.doi.org/10.18388/abp.2018_2767DOI Listing
April 2019
3 Reads

Dietary quercetin abrogates hepatorenal oxidative damage associated with dichloromethane exposure in rats.

Acta Biochim Pol 2019 Apr 8. Epub 2019 Apr 8.

Industrial and Nutritional Biochemistry, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria.

Exposure to dichloromethane (DCM), a commonly used chlorinated solvent in industrial settings and for the production of many household products, reportedly elicits detrimental effects in animals and humans. The present study investigated the protective role of dietary quercetin on DCM-induced hepatorenal damage in rats. Experimental rats were orally administered with DCM (150 mg/kg) and 30 min later with quercetin at 10, 20 and 40 mg/kg or none for 7 consecutive days. Read More

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http://dx.doi.org/10.18388/abp.2018_2771DOI Listing

Computed tomography indicators of cerebral microperfusion improve long term after carotid stenting in symptomatic patients.

Acta Biochim Pol 2019 Apr 8. Epub 2019 Apr 8.

2-nd Department of Radiology, Medical University of Gdansk, Gdańsk, Poland.

Objectives: We tested the hypothesis that computed tomography (CT) perfusion markers of cerebral microcirculation would improve 36 months after internal carotid artery stenting for symptomatic carotid stenosis while results obtained 6-8 weeks after the stenting procedure would yield a predictive value.

Methods: We recruited consecutive eligible patients with >70% symptomatic carotid stenosis with a complete circle of Willis and normal vertebral arteries to the observational cohort study. We detected changes in the cerebral blood flow (CBF), cerebral blood volume (CBV), mean transit time (MTT), time to peak (TTP) and permeability surface area-product (PS) before and after carotid stenting. Read More

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https://ojs.ptbioch.edu.pl/index.php/abp/article/view/2793
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http://dx.doi.org/10.18388/abp.2018_2793DOI Listing
April 2019
2 Reads

Genistein modulates gene activity in psoriatic patients.

Acta Biochim Pol 2019 Feb;66(1):101-110

Department of Medical Biology and Genetics, University of Gdańsk, Gdańsk, Poland; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Laboratory of Molecular Biology, Gdańsk, Poland.

Despite the impressive advancements in the treatment of psoriasis over the past two decades, there is still a need for further improvement. As previously shown in the literature, genistein (5,7-dihydroxy-3-(4-hydroxyphenyl)chromen-4-one), naturally occurring plant compound displays multidirectional action, also in relation to alleviating psoriasis symptoms. In this work we focused our attention on genistein impact on expression of genes when treating moderate-to-severe psoriasis patients. Read More

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http://dx.doi.org/10.18388/abp.2018_2772DOI Listing
February 2019

Amyloids, Congo red and the apple-green effect.

Acta Biochim Pol 2019 Mar;66(1):39-46

Department of Bioinformatics and Telemedicine, Jagiellonian University - Medical College, Krakow, Poland.

This paper attempts to find evidence of the previously proposed opinion that amyloids complex with Congo red molecules which preserve their supramolecular organization. As evidence of the overpowering tendency of Congo red molecules to self-assemble, we present an increasing acidity of molecules that follows increasing concentration of the dye, and a highly notable nonlinear increase in absorbance in the UV band (300-400 nm). This effect is analyzed in a model where the amyloid fibril is simulated by polyvinyl alcohol, providing a scaffold to stabilize a long Congo red micelle. Read More

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https://ojs.ptbioch.edu.pl/index.php/abp/article/view/2667
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http://dx.doi.org/10.18388/abp.2018_2667DOI Listing
March 2019
4 Reads

Assessment of fracture healing properties of lovastatin loaded nanoparticles: preclinical study in rat model.

Acta Biochim Pol 2019 Mar;66(1):71-76

Department of Orthopaedics, Hanzhong People's Hospital, Hantai District, Hanzhong City, Shaanxi Province, 723000, China.

Bone fracture, being mainly caused by mechanical stress, requires special and quick attention for a rapid healing. The study presented here aims at formulating nanoparticulate system to overcome the solubility issues of lovastatin. The lovastatin nanoparticles were successfully prepared by ionotropic gelation method using chitosan and tri-polyphosphate as polymers. Read More

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http://dx.doi.org/10.18388/abp.2018_2719DOI Listing
March 2019
2 Reads

Effects of starvation and refeeding on growth performance and stress defense mechanisms of stellate sturgeon Acipenser stellatus juveniles from aquaculture.

Acta Biochim Pol 2019 Mar;66(1):47-59

University of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, Bucharest, Romania.

Acipenser stellatus represents a species of great economical interest due to its roe used for caviar production. Therefore, it has been intensively captured for decades and nowadays, this species is on the verge of extinction. As a consequence, Acipenser stellatus is intensively raised in fish farms. Read More

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http://dx.doi.org/10.18388/abp.2018_2712DOI Listing
March 2019
1 Read

Immunomodulatory potential of gut microbiome-derived short-chain fatty acids (SCFAs).

Acta Biochim Pol 2019 Mar;66(1):1-12

Department of Histology and Developmental Biology, Pomeranian Medical University, Szczecin, Poland.

Intestinal microbiota is an element of the bacterial ecosystem in all mammalian organisms. These microorganisms play a very important part in the development, functioning, and modulation of the immune system from the moment of birth. In recent years, owing to the use of modern sequencing techniques, the microbiome composition in healthy people has been identified based on bacterial 16S rRNA analysis. Read More

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http://dx.doi.org/10.18388/abp.2018_2648DOI Listing
March 2019
2 Reads

Pseudomonas aeruginosa alkaline protease exhibits a high renaturation capability.

Acta Biochim Pol 2019 Mar;66(1):91-100

Department of Immunobiology, Maria Curie-Sklodowska University, Lublin, Poland.

Thermally induced unfolding and renaturation capability of alkaline proteases (AprA) of three Pseudomonas aeruginosa strains, i.e. ATCC 27853 and two clinical isolates, was examined. Read More

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http://dx.doi.org/10.18388/abp.2018_2741DOI Listing
March 2019
1 Read

Sildenafil does not affect the proliferation of human lymphocytes in the in vitro transplant model.

Acta Biochim Pol 2019 Feb;66(1):115-117

Department of Clinical Immunology, Transplantation Institute, Medical University of Warsaw, Warsaw, Poland.

Sildenafil is used in the treatment of erectile dysfunction and pulmonary arterial hypertension. Numerous  studies revealed beneficial effects of its use in renal, liver, heart and bone marrow transplant recipients. Some reports suggested that the drug modulates the function of the immune system, however, its influence on antigen-induced proliferation of lymphocytes remains unknown. Read More

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http://dx.doi.org/10.18388/abp.2018_2646DOI Listing
February 2019
3 Reads

Health benefits of resveratrol administration.

Acta Biochim Pol 2019 Feb;66(1):13-21

Faculty of Medicine, Rzeszów University, Rzeszów, Poland.

Resveratrol is a polyphenol that is abundant in grape skin and seeds. Food sources of resveratrol include wine, berries, and peanuts. This compound has many properties, including activity against glycation, oxidative stress, inflammation, neurodegeneration, several types of cancer, and aging. Read More

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http://dx.doi.org/10.18388/abp.2018_2749DOI Listing
February 2019
3 Reads

Identification of six novel factor viii gene variants using next generation sequencing and molecular dynamics simulation.

Acta Biochim Pol 2019 Feb;66(1):23-31

1Department of Medical Genetics, Faculty of Medicine, Umm Al-Qura University, P.O. Box 715, Makkah 21955, Saudi Arabia; 2Science and Technology Unit, Umm Al Qura University, P.O. Box 715, Makkah 21955, Saudi Arabia.

Hemophilia A is an X-linked recessive hemorrhagic disorder caused by variants in the F8 gene. To identify known and novel causative variants in hemophilia A, we have carried out genetic analysis among Saudi patients. Twenty-one patients, who were negative for inv-1/inv-22, were selected for analysis by next generation sequencing, thereafter confirmed by Sanger sequencing. Read More

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http://dx.doi.org/10.18388/abp.2018_2339DOI Listing
February 2019
1 Read
1.153 Impact Factor

Prognostic value of broad-spectrum keratin clones AE1/AE3 and CAM5.2 in small cell lung cancer patients undergoing pulmonary resection.

Acta Biochim Pol 2019 Feb;66(1):111-114

7Department of Oncology and Radiotherapy, Medical University of Gdansk, Poland.

Introduction: Small cell lung carcinoma (SCLC) is an aggressive pulmonary neoplasm of neuroendocrine origin. Keratins form a large group of intermediate filaments, which are major structural proteins in epithelial cells and carcinomas. SCLC shows a wide spectrum of keratin expression, from very strong to completely negative. Read More

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http://dx.doi.org/10.18388/abp.2018_2773DOI Listing
February 2019
1 Read

Association between different types of plant-based diets and leptin levels in healthy volunteers.

Acta Biochim Pol 2019 Feb;66(1):77-82

of Clinical Nutrition and Dietetics, Faculty of Health Sciences, Medical University of Gdańsk, Gdańsk, Poland.

Leptin is an important factor regulating appetite and energety metabolism; disturbances in its signaling are related to adiposity and contribute to the excessive body fat. About a third of the human population is overweight or suffers from obesity, as well as from associated medical conditions. It is well established that vegetarian, especially vegan, diet is very effective in lowering BMI and body fat, thus, plant-based diets are associated with a lower risk of obesity. Read More

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http://dx.doi.org/10.18388/abp.2018_2725DOI Listing
February 2019
2 Reads

Periplasmic expression of a restriction endonuclease in Escherichia coli and its effect on the antiviral activity of the host.

Acta Biochim Pol 2019 Feb;66(1):83-89

University of Gdansk, Department of Microbiology, Wita Stwosza 59, 80-308 Gdańsk, Poland.

One possible mechanism preventing phage infection of the bacterial cells is related to the presence of an effective restriction-modification system (R-M) which allows restriction of the invading DNA. However, there are some limitations to the absolute restriction of foreign DNA. Since there is a serious conflict between increase in the restriction-modification genes expression level and cell viability, we examined the antiviral effect of EcoRI restriction endonuclease after its translocation to the periplasmic space of the cell. Read More

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http://dx.doi.org/10.18388/abp.2018_2740DOI Listing
February 2019

Identification and partial characterization of proteolytic activity of Enterococcus faecalis relevant to their application in dairy industry.

Acta Biochim Pol 2019 Feb;66(1):61-69

Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Poznań, Poland.

Bacteria of the genus Enterococcus are lactic acid bacteria (LAB), which occur ubiquitous in many traditional fermented foods, especially artisanal cheeses, playing positive role in the development of cheese flavor. Moreover, several enterococci are successfully used as a pharmaceutical probiotic and some of them are able to produce bacteriocin and bioactive peptides, thanks to which the possibilities of application of enterococci in dairy technology and biotechnology are increased. The aims of the study were to investigate the proteolytic potential and identify the key enzymes of proteolytic system of Enterococcus faecalis isolated from artisan Polish cheeses. Read More

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http://dx.doi.org/10.18388/abp.2018_2714DOI Listing
February 2019

Rapid and easy detection of the five most common mutations in BRCA1 and BRCA2 genes in the Polish population using CAPS and ACRS-PCR methods.

Acta Biochim Pol 2019 Feb;66(1):33-37

Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland.

In this publication we present a fast method of diagnosing the most common polymorphisms of BRCA1 and BRCA2 genes in Poland - C61G [c.300T>G], C64R [c.190T>C], 4153delA [c. Read More

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http://dx.doi.org/10.18388/abp.2018_2654DOI Listing
February 2019

Identification of evolutionary conserved DNA sequence and corresponding S21 ribosomal protein region for diagnostic purposes of all Borrelia spirochetes.

Acta Biochim Pol 2019 Jan;66(1):119-122

Department of Molecular Biotechnology and Microbiology, Gdansk University of Technology, Gdańsk, Poland.

It is still under investigation, whether all Borrelia sp. causing Lyme borreliosis and other diseases are already identified and properly classified as human pathogens. For this reason, it is of great importance to develop a diagnostic ELISA test that detects all Borrelia sp. Read More

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http://dx.doi.org/10.18388/abp.2018_2657DOI Listing
January 2019
7 Reads

Plasma levels of VEGF-A, VEGF B, and VEGFR-1 and applicability of these parameters as tumor markers in diagnosis of breast cancer.

Acta Biochim Pol 2018 Dec;65(4):621-628

Medical University of Bialystok.

The VEGF family members are important factors in promoting angiogenesis and lymphangiogenesis in malignant processes. The aim of this study was to investigate plasma concentrations of VEGF-A, VEGF-B and their soluble VEGFR-1 receptor and their diagnostic utility and potency as compared to CA 15-3 in breast cancer patients and in relation to the control group. The study included 120 breast cancer patients and 60 control patients. Read More

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http://dx.doi.org/10.18388/abp.2018_2713DOI Listing
December 2018
3 Reads

The inhibitory effects of rosmarinic acid on catabolism induced by IL-1β in rat chondrocyte.

Acta Biochim Pol 2018 Dec;65(4):535-538

Department of Orthopedics Surgery, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, People's Republic of China.

The effects of rosmarinic acid (RosA) on osteoarthritis (OA) was investigated in rat chondrocytes. Chondrocytes were isolated from rat cartilage, incubated with RosA in the presence of interleukin-1beta (IL-1β) (10 ng/ml). The production of IL-6, as well as the mRNA level of aggrecan (ACAN) and collagen 2 (COL2), were assessed. Read More

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http://dx.doi.org/10.18388/abp.2018_2607DOI Listing
December 2018

The promises and risks of probiotic Bacillus species.

Acta Biochim Pol 2018 12;65(4):509-519

Uniwersytet Gdański Wydział Chemii Katedra Biotechnologii Molekularnej ul. Wita Stwosza 63 80-308 Gdańsk.

Supplementing the human microbiome with probiotic microorganisms is a proposed solution for civilization syndromes such as dysbiosis and gastrointestinal tract (GIT) disorders. Bimodal probiotic strains of the Bacillus genus constitute the microbiota of the human environment, and are typically found in soil, water, a number of non-dairy fermented foods, as well as in human and animal GIT. Probiotic Bacillus sp. Read More

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http://dx.doi.org/10.18388/abp.2018_2652DOI Listing
December 2018
3 Reads

Protein-energy wasting and asymmetric dimethylarginine in peritoneal dialysis patients.

Acta Biochim Pol 2018 Dec;65(4):581-584

Department of Nephrology, Transplantology and Internal Medicine, Medical University of Gdansk, Gdańsk, Poland.

Cardiovascular (CVS) morbidity and mortality in the peritoneal dialysis patients (PD) is 10-30-fold higher than in the general population. A relatively low level of adiponectin and a higher level of leptin are important predictors of vascular complications as well as CVS events in PD patients. The asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide synthase, is an important risk factor of CVS morbidity and mortality. Read More

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https://ojs.ptbioch.edu.pl/index.php/abp/article/view/2634
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http://dx.doi.org/10.18388/abp.2018_2634DOI Listing
December 2018
17 Reads

Kidney injury by cyclosporine A is aggravated in heme oxygenase-1 deficient mice and involves regulation of microRNAs.

Acta Biochim Pol 2018 Nov;65(4):613-620

1Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland; 2Kardio-Med Silesia, Zabrze, Poland.

Cyclosporine A (CsA), a widely used immunosuppressive drug, exerts nephrotoxic activities, as demonstrated by increased tubulointerstitial fibrosis, inflammation and podocyte damage. Recently, a number of microRNAs expressed in the kidney have been reported to be elevated during renal damage. Our aim was to investigate the effect of CsA on selected microRNAs in the mouse kidney after CsA treatment. Read More

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https://ojs.ptbioch.edu.pl/index.php/abp/article/view/2658
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http://dx.doi.org/10.18388/abp.2018_2658DOI Listing
November 2018
10 Reads

Aquaporins in human platelets: intracellular localization and possible role in granule and lysosome secretion.

Acta Biochim Pol 2018 Nov;65(4):555-566

Department of Physical Chemistry Medical University of Bialystok.

This study was undertaken to establish the presence and the role of aquaporins (AQPs) in human platelets. Immunodetection with polyclonal antibodies and fluorescent microscopy suggest the presence of AQP isoforms - 0-7 and 9-12 - localized (in resting platelets) in the plasma membrane and in the dense and alpha granules. In thrombin- or monensin-treated platelets, the granules' AQPs become visible in the whole cell body, indicating the granules' swelling. Read More

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http://dx.doi.org/10.18388/abp.2018_2621DOI Listing
November 2018
15 Reads

Structural analysis of the Aβ(15-40) amyloid fibril based on hydrophobicity distribution.

Acta Biochim Pol 2018 Nov;65(4):595-604

Jagiellonian University - Medical College.

The Aβ42 amyloid is the causative factor behind various neurodegenerative processes. It forms elongated fibrils which cause structural devastation in brain tissue. The structure of an amyloid seems to be a contradiction of protein folding principles. Read More

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https://ojs.ptbioch.edu.pl/index.php/abp/article/view/2647
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http://dx.doi.org/10.18388/abp.2018_2647DOI Listing
November 2018
5 Reads

Usefulness of the Triglycerides to High-Density Lipoprotein Cholesterol ratio (TG/HDL-C) in prediction of metabolic syndrome in Polish obese children and adolescents.

Acta Biochim Pol 2018 11;65(4):605-611

Department of Paediatric and Endocrinology, Medical Univesity of Warsaw, Warszawa, Poland.

The triglycerides to high-density lipoprotein cholesterol ratio (TG/HDL-C) is a useful surrogate marker of insulin resistance and cardiovascular risk factors. We aimed to assess the relationship between the TG/HDL-C ratio and insulin resistance (IR) and its usefulness in prediction of the metabolic syndrome (MS). This retrospective study involved 122 obese children with the mean age of 11. Read More

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http://dx.doi.org/10.18388/abp.2018_2649DOI Listing
November 2018
16 Reads

The role of eicosanoids in renal diseases - potential therapeutic possibilities.

Acta Biochim Pol 2018 Nov;65(4):479-486

Pomeranian Medical University, Department of Nephrology, Transplantology and Internal Medicine.

Eicosanoids are biologically active molecules that are created in the process of oxidation of arachidonic acid (AA) which is a constituent of the cell membrane phospholipids. Throughout the years it was evidenced by experiments that the lipid and lipid-derived metabolites play an important role in physiological and pathological processes in the kidneys. They are being considered as biomarkers in detecting acute kidney injury, nephrotoxicity, glomerulonephritis and early stages of diabetic nephropathy because of their participation in inflammatory processes and in oxidative stress. Read More

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http://dx.doi.org/10.18388/abp.2018_2609DOI Listing
November 2018
16 Reads

Protamine-like proteins have bactericidal activity. The first evidence in Mytilus galloprovincialis.

Acta Biochim Pol 2018 11;65(4):585-594

Dipartimento di Biologia, Università degli Studi di Napoli Federico II. Napoli, Italy.

The major acid-soluble protein components of the mussel Mytilus galloprovincialis sperm chromatin consist of the protamine-like proteins PL-II, PL-III and PL-IV, an intermediate group of sperm nuclear basic proteins between histones and protamines. The aim of this study was to investigate the bactericidal activity of these proteins since, to date, there are reports on bactericidal activity of protamines and histones, but not on protamine-like proteins. We tested the bactericidal activity of these proteins against Gram-positive bacteria: Enterococcus faecalis and two different strains of Staphylococcus aureus, as well as Gram-negative bacteria: Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella typhmurium, Enterobacter aerogenes, Enterobacter cloacae, and Escherichia coli. Read More

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http://dx.doi.org/10.18388/abp.2018_2638DOI Listing
November 2018
14 Reads

Characteristics of virophages and giant viruses.

Acta Biochim Pol 2018 Oct;65(4):487-496

Department of Microbiology, Faculty of Biology, University of Szczecin, Poland.

Five years after being discovered in 2003, some giant viruses were demonstrated to play a role of the hosts for virophages, their parasites, setting out a novel and yet unknown regulatory mechanism of the giant viruses presence in an aqueous. So far, 20 virophages have been registered and 13 of them have been described as a metagenomic material, which indirectly impacts the number of single- and multi-cell organisms, the environment where giant viruses replicate. Read More

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http://dx.doi.org/10.18388/abp.2018_2631DOI Listing
October 2018

Significance of NF-kappaB signaling and PARP1 activity in the TNF-induced inhibition of PHEX gene expression in human osteoblasts.

Acta Biochim Pol 2018 Nov;65(4):573-571

Department of Animal Physiology, Institute of Zoology, Faculty of Biology, University of Warsaw, Warsaw, Poland.

Although loss of bone mineral density is a common symptom of chronic inflammatory diseases, its mechanisms are still poorly understood. The PHEX gene encodes a Zn-endopeptidase expressed in osteoblasts and contributes to bone mineralization. Data derived from rodents has indicated co-repression of the PHEX gene by the NF-κB pathway and poly(ADP-ribose) polymerase 1 (PARP1). Read More

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http://dx.doi.org/10.18388/abp.2018_2633DOI Listing
November 2018
10 Reads

Circular and long non-coding RNAs and their role in ophthalmologic diseases.

Acta Biochim Pol 2018 Nov;65(4):497-508

Department of Ophthalmology, Poznan University of Medical Sciences, Poznan, Poland.

Long non-coding RNAs are 200 nucleotide long RNA molecules which lack or have limited protein-coding potential. They can regulate protein formation through several different mechanisms. Similarly, circular RNAs are reported to play a critical role in post-transcriptional gene regulation. Read More

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http://ojs.ptbioch.edu.pl/index.php/abp/article/view/2620
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http://dx.doi.org/10.18388/abp.2018_2639DOI Listing
November 2018
15 Reads

Molecular characterization of central cytoplasmic loop in Aspergillus nidulans AstA transporter.

Acta Biochim Pol 2018 Nov;65(4):545-554

Institute of Biochemistry and Biophysics PAS, Pawinskiego str. 5a, 02-106 Warsaw, POLAND.

AstA (alternative sulfate transporter) belongs to a large, but poorly characterized, Dal5 family of allantoate permeases of the Major Facilitator Superfamily. The astA gene has been cloned from an IAM 2006 Japanese strain of Aspergillus nidulans by complementation of a sulfate permease-deficient mutant. In this study we show that conserved lysine residues in Central Cytoplasmic Loop (CCL) of the AstA protein may participate in anion selectivity, and control kinetic properties of the AstA transporter. Read More

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http://ojs.ptbioch.edu.pl/index.php/abp/article/view/2620
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http://dx.doi.org/10.18388/abp.2018_2620DOI Listing
November 2018
12 Reads

Estimation of gamma-glutamyl transferase as a suitable simple biomarker of the cardiovascular risk in children with non-alcoholic fatty liver disease.

Acta Biochim Pol 2018 Oct;65(4):539-544

Medical University of Bialystok.

Non-alcoholic fatty liver disease (NAFLD) is a hepatic manifestation of metabolic syndrome and a risk factor for cardiovascular disease (CVD). Research conducted in adults has proven that GGT can also be an independent risk factor for CVD. The aim of this study was to ascertain if GGT can be regarded as a simple biomarker of cardiovascular risk in obese children with NAFLD. Read More

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http://abpnew.ptbioch.edu.pl/index.php/abp/article/view/2611
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http://dx.doi.org/10.18388/abp.2018_2611DOI Listing
October 2018
9 Reads

In silico analysis of different signal peptides to discover a panel of appropriate signal peptides for secretory production of Interferon-beta 1b in Escherichia coli.

Acta Biochim Pol 2018 Oct;65(4):521-534

Department of Animal Sciences, University of Guilan, Rasht, Iran.

Signal peptides (SPs) are one of the most important factors for suitable secretion of the recombinant  heterologous proteins in Escherichia coli (E. coli). The objective of this study was to identify a panel of signal peptides (among the 90 biologically active SPs) required for the secretory production of interferon-beta 1b (IFN-beta 1b) recombinant protein into the periplasmic space of E. Read More

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http://ojs.ptbioch.edu.pl/index.php/abp/article/view/2351
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http://dx.doi.org/10.18388/abp.2018_2351DOI Listing
October 2018
2 Reads

Severe clinical toxicity caused by 25I-NBOMe confirmed analytically using LC-MS-MS method.

Acta Biochim Pol 2018 Oct;65(4):567-571

Department of Clinical Toxicology, Medical University of Gdansk, Gdansk, Poland; Pomeranian Center of Toxicology, Gdansk, Poland.

Rhabdomyolysis is a relatively rare, but potentially serious complication of various diseases. Muscular injury and resultant release of electrolytes, myoglobin and other enzymatic proteins e.g. Read More

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http://dx.doi.org/10.18388/abp.2018_2627DOI Listing
October 2018

Role of Apolipoprotein E gene polymorphism in the risk of familial hypercholesterolemia: a case-control study.

Acta Biochim Pol 2018 15;65(3):415-420. Epub 2018 Sep 15.

Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, PO Box-10219, Riyadh-11433, Kingdom of Saudi Arabia.

Familial Hypercholesterolemia (FH) is characterized by elevated cholesterol and based on biochemical, clinical, and genetic studies and FH disease, which was documented even with limited mutations. Earlier studies focused on Apolipoprotein E (ApoE) in variable diseases. The current study aimed to investigate the genetic association between FH disease and ApoE gene polymorphisms (rs429358 and rs7412) in the Saudi population. Read More

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http://dx.doi.org/10.18388/abp.2017_2344DOI Listing
December 2018
2 Reads

Chlamydial genomic MinD protein does not regulate plasmid-dependent genes like Pgp5.

Acta Biochim Pol 2018 15;65(3):425-429. Epub 2018 Sep 15.

Department of Dermatovenereology, Tianjin Medical University General Hospital, Tianjin 300052, China.

Chlamydia has a unique intracellular developmental cycle, which has hindered the single protein function study of Chlamydia. Recently developed transformation system of Chlamydia has greatly advanced the chlamydial protein's function research and was used to find that a chlamydial plasmid-encoded Pgp5 protein can down-regulate plasmid-dependent genes. It is assumed, that chlamydial genomic MinD protein has a similar function to Pgp5. Read More

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http://dx.doi.org/10.18388/abp.2017_2360DOI Listing
December 2018
2 Reads

Influence of oleic acid in different solvent media on BRL 3A cell growth and viability.

Acta Biochim Pol 2018 15;65(3):443-447. Epub 2018 Sep 15.

College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, China.

Oleic acid (OA) is widely used in pathology studies of hepatocellular lipid deposition. Identifying the effects of different solvents on OA-induced liver lipid deposition would be beneficial for studies on hepatocytes. We treated BRL 3A cells with OA dissolved in different solvents. Read More

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http://www.actabp.pl/#File?./html/3_2018/2018_2557.html
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http://dx.doi.org/10.18388/abp.2018_2557DOI Listing
December 2018
13 Reads

NLCs as a potential carrier system for transdermal delivery of forskolin.

Acta Biochim Pol 2018 15;65(3):437-442. Epub 2018 Sep 15.

Faculty of Chemical Engineering and Technology, Institute of Organic Chemistry and Technology, Cracow University of Technology, Kraków, Poland.

Nanostructured lipid carriers (NLC) composed of the substances generally recognized as safe (GRAS) were obtained by using a hot high-pressure homogenization technique (HPH). The influence of the number of homogenization cycles and concentration of a decyl glucoside surfactant on the NLC properties were studied. The system's stability was assessed by macroscopic observation, light backscattering and zeta potential measurements. Read More

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http://www.actabp.pl/#File?./html/3_2018/2018_2554.html
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http://dx.doi.org/10.18388/abp.2018_2554DOI Listing
December 2018
9 Reads

Association of HindIII-polymorphism in kappa-casein gene with milk, fat and protein yield in holstein cattle.

Acta Biochim Pol 2018 15;65(3):403-407. Epub 2018 Sep 15.

Department of Genetics and Breeding Biology, Slovak University of Agriculture in Nitra, Nitra, Slovakia.

The aim of this paper was to evaluate the effect of genetic polymorphism of kappa-casein on milk production in Holstein cattle. Two hundred and ten Holstein cows were used in this study. We established genotype structure of cattle population and calculated allelic frequencies based on PCR-RFLP analyses. Read More

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http://dx.doi.org/10.18388/abp.2017_2313DOI Listing
December 2018
1 Read

Ezetimibe prevents myocardial remodeling in an obese rat model by inhibiting inflammation.

Acta Biochim Pol 2018 8;65(3):465-470. Epub 2018 Sep 8.

Department of Emergency, Chest Pain Center, Qilu Hospital, Shandong University, Jinan, Shandong, China.

Inflammation plays an important role in the development of many obesity-related diseases. This study aimed to investigate the effect of ezetimibe on inflammation and myocardial remodeling in obese rats. A rat model of obesity was established, and myocardial damage was examined by transmission electron microscopy and Masson staining. Read More

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http://www.actabp.pl/#File?./html/3_2018/2018_2597.html
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http://dx.doi.org/10.18388/abp.2018_2597DOI Listing
December 2018
16 Reads
1.153 Impact Factor

Overexpression of zeaxanthin epoxidase gene from Medicago sativa enhances the tolerance to low light in transgenic tobacco.

Acta Biochim Pol 2018 8;65(3):431-435. Epub 2018 Sep 8.

Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.

Zeaxanthin epoxidase (ZEP) plays an important role in xanthophyll cycle which is a process closely related to photosynthesis. However, an impact of ZEP on low-light stress has not been studied. In this study, the functions of an alfalfa (Medicago sativa) zeaxanthin epoxidase gene, MsZEP, in response to low-light stress were investigated by heterologous expression in tobacco (Nicotiana tabacum). Read More

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http://dx.doi.org/10.18388/abp.2018_2551DOI Listing
December 2018
4 Reads

Characterization of an acidic α-galactosidase from hemp (Cannabis sativa L.) seeds and its application in removal of raffinose family oligosaccharides (RFOs).

Acta Biochim Pol 2018 8;65(3):383-389. Epub 2018 Sep 8.

School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.

An acidic α-galactosidase designated as hemp seed α-galactosidase (HSG) was purified from hemp (Cannabis sativa L.) seeds. By means of chromatographic procedures which involved chromatography on the cation-exchangers CM-cellulose and SP-Sepharose, chromatography on the anion-exchangers DEAE-cellulose and Q-Sepharose, and gel filtration on Superdex 75 using fast protein liquid chromatography, HSG was purified to electrophoretic homogeneity. Read More

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http://dx.doi.org/10.18388/abp.2017_1535DOI Listing
December 2018

Association between RBMS1 gene rs7593730 and BCAR1 gene rs7202877 and Type 2 diabetes mellitus in the Chinese Han population.

Acta Biochim Pol 2018 8;65(3):377-382. Epub 2018 Sep 8.

Department of Endocrinology and Metabolism, the Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China.

Two recent studies found that RBMS1 gene rs7593730 and BCAR1 gene rs7202877 are related to type 2 diabetes. However, the association of these loci with type 2 diabetes mellitus (T2DM) has not been examined in Chinese. We performed a replication study to investigate the association of the 2 susceptibility loci with T2DM in the Chinese population. Read More

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http://dx.doi.org/10.18388/abp.2017_1451DOI Listing
December 2018
2 Reads

Studies on stability of emulsions containing carotenoids.

Acta Biochim Pol 2018 29;65(3):455-463. Epub 2018 Aug 29.

Faculty of Cosmetology and Educational Sciences, College of Health and Beauty Education, Poznań, Poland.

Emulsions are commonly used to improve aesthetics but they may also be applied to deliver active ingredients. Recently, a group of pigments known as carotenoids has been added to emulsions as an active compound mainly due to their antioxidant properties. The stability of these formulations is a critical parameter for both, pharmaceutical and cosmetics industry. Read More

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http://dx.doi.org/10.18388/abp.2018_2589DOI Listing
December 2018
1 Read

Bradykinin B2 receptor and dopamine D2 receptor cooperatively contribute to the regulation of neutrophil adhesion to endothelial cells.

Acta Biochim Pol 2018 27;65(3):367-375. Epub 2018 Aug 27.

Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in Krakow, Kraków, Poland.

Leukocyte adhesion to the vascular endothelium contributes to many immunological and inflammatory disorders. These processes have been shown to be mediated by bradykinin receptor type 2 (B2R) and dopamine receptor type 2 (D2R). In a previous study, we reported the formation of a B2R-D2R heterodimer, possibly altering cellular functions. Read More

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http://www.actabp.pl/#File?./html/3_2018/2018_2641.html
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http://dx.doi.org/10.18388/abp.2018_2641DOI Listing
December 2018
18 Reads