4,260 results match your criteria Acta Biochimica Polonica[Journal]

miR-379-5p inhibits proliferation and invasion of the endometrial cancer cells by inhibiting expression of ROR1.

Acta Biochim Pol 2021 Jun 11. Epub 2021 Jun 11.

Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, 430022, China.

Endometrial cancer is a common gynecological malignancy, and the incidence of this disease has increased in recent years. Recently, some studies suggested that the expression of miR-379-5p suppressed the metastasis of breast cancer cells. However, whether the expression of miR-379-5p could affect the proliferation, migration and invasion of endometrial cancer is unclear. Read More

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USP28 promotes aerobic glycolysis of colorectal cancer by increasing stability of FOXC1.

Acta Biochim Pol 2021 Jun 9. Epub 2021 Jun 9.

Department of Anorectal Surgery, First People's Hospital of Yuhang District, Hangzhou, Hangzhou City, Zhejiang Province, 311100, P.R. China.

Aerobic glycolysis is essential for cancer cell metabolism and growth. Deubiquitinase, USP28 (ubiquitin specific peptidase 28), could maintain stability of proteins involved in tumor progression. This study was performed to investigate the role of USP28 in aerobic glycolysis of colorectal cancer. Read More

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Ononin induces cell apoptosis and reduces inflammation in rheumatoid arthritis fibroblast-like synoviocytes by alleviating MAPK and NF-κB signaling pathways.

Acta Biochim Pol 2021 Jun;68(2):239-245

Department of Joint Surgery, The Fifth Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong Province, 510900, China.

As a kind of chronic inflammatory diseases, Rheumatoid arthritis (RA) has a low cure rate and easy recurrence. It has widely reported that abnormal activation of mitogen-activated protein kinase (MAPK) and nuclear factor kappa-B (NF-κB) signaling pathways are associated with the development of RA inflammation. Blocking the inflammatory signaling pathways of rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) can delay the development of RA. Read More

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Overexpression of miR-874-3p alleviates LPS-induced apoptosis and inflammation in alveolar epithelial cell by targeting EGR3/NF-κB.

Acta Biochim Pol 2021 May;68(2):231-238

Department of Infection, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China.

Objective: MicroRNA (miRNA) is implicated in the pathogenic mechanism of pneumonia. Role of miR-874-3p in pediatric pneumonia was therefore evaluated in this study.

Methods: Expression levels of miR-874-3p in the serum samples from pediatric patients with pneumonia and LPS-treated HPAEpiC were determined by RT-qPCR (reverse transcription quantitative real-time PCR). Read More

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The effect of miR-496 on eIF3h in lung invasive adenocarcinoma.


Acta Biochim Pol 2021 May 26. Epub 2021 May 26.

Department of Cardiothoracic Surgery, Changzhou No. 2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, Changzhou, Jiangsu 213000, P.R. China.

This study explores the correlation between microRNA-496 (miR-496) expression and eukaryotic translation initiation factor 3H (eIF3h) expression in lung invasive adenocarcinoma. A total of 71 patients with lung adenocarcinoma were included in the study. The tumor tissue samples were collected together with the normal lung tissue samples that were acquired more than 2 cm away from the edge of the tumor). Read More

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Ginsenoside Rg3 suppresses ovarian cancer cell proliferation and invasion by inhibiting the expression of lncRNA H19.

Acta Biochim Pol 2021 May 26. Epub 2021 May 26.

1Department of Gynecologic Oncology, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Gongshu District, Hangzhou City, Zhejiang Province, 310000, China; 2Institute of Cancer and Basic Medicine, IBMC, Chinese Academy of Sciences, China.

Ovarian cancer (OC) is the most malignant disease of the female reproductive system and accounts for a large proportion of gynecological cancer-related deaths. Emerging evidence has indicated that ginsenoside Rg3, one of the tetracyclic triterpenoid saponins in ginseng, plays crucial roles in regulating cancer progression, yet its role and mechanisms in regulating the proliferation and invasion of OC are still elusive. In this study, the cell viability, proliferation, migration and invasion of OC were assessed by using methyl thiazol tetrazolium (MTT), colony formation, wound healing and Transwell assays, respectively. Read More

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The upregulation of miR-204-3p in LPS-induced acute lung injury aggravated pulmonary endothelial cells apoptosis via targeting sulfatase 2.

Acta Biochim Pol 2021 May;68(2):217-222

Department of Respiratory Medicine, Nanjing Medical University Affiliated Changzhou No. 2 People's Hospital, Changzhou, Jiangsu Province, 213164, China.

Acute lung injury (ALI) results from the injury of alveolar epithelial cells and pulmonary capillary endothelial cells, with a high mortality rate ranging from 29% to 42%. Therefore, more efficient therapeutic strategies for ALI are necessary. Numerous studies revealed that miRNAs play a role in the regulation of ALI. Read More

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Meisoindigo inhibits cellular proliferation via down-regulation of the PI3K/Akt pathway and induces cellular apoptosis in glioblastoma U87 cells.

Acta Biochim Pol 2021 05;68(2):309-315

1Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China; 2Central laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China.

Objective: The current study was to explore whether meisoindigo was effective in suppressing proliferation and inducing apoptosis of human glioblastoma multiforme U87 cells and to explore its possible mechanisms.

Method: Morphological changes were observed by light microscopy. Cell counting kit-8 (CCK-8) assay was performed to detect cellular proliferation. Read More

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Upregulation of miR-1266-5p serves as a prognostic biomarker of hepatocellular carcinoma and facilitates tumor cell proliferation, migration and invasion.

Acta Biochim Pol 2021 May;68(2):293-300

Imaging Center, Affiliated Hospital of Weifang Medical University, Weifang 261031, Shandong, Chin.

Objective: Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death worldwide. This study aimed to analyze the prognostic value of microRNA-1266-5p (miR-1266-5p) in HCC patients and investigate its biological function in HCC progression.

Methods: The expression of miR-1266-5p in tissues and cells was measured by quantitative real-time PCR (qRT-PCR). Read More

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Analysis of the complete mitochondrial genome sequence of the resurrection plant Haberlea rhodopensis.

Acta Biochim Pol 2021 May;68(2):277-286

1Institute of Molecular Biology and Biotechnology, Plovdiv, Bulgaria; 2Centre of Plant Systems Biology and Biotechnology, Plovdiv, Bulgaria.

Haberlea rhodopensis is a paleolithic tertiary relict species that belongs to the unique group of resurrection plants sharing remarkable tolerance to desiccation. When exposed to severe drought stress, this species shows an ability to maintain structural integrity of its deactivated photosynthetic apparatus, which easily reactivates upon rehydration. In addition to its homoiochlorophyllous nature, the resurrection capability of H. Read More

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The influence of dietary inclusion of wood vinegar supplementation on growth performance, nutrient digestibility, and meat quality in grower-finisher pigs.

Acta Biochim Pol 2021 May;68(2):287-292

Department of Animal Resource and Science, Dankook University, No. 29 Anseodong, Cheonan, Choongnam, 330-714, South Korea.

The current research aimed to evaluate the effects of dietary inclusion of wood vinegar on growth performance, nutrient digestibility, and meat quality of grower-finisher pigs. In total, 132 crossbred ({Landrace × Yorkshire × Duroc}) grower-finisher pigs with an initial average body weight 30.48±4. Read More

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A rapid method for protein staining in polyacrylamide gels using water saturated with chloroform.

Acta Biochim Pol 2021 May;68(2):347-351

Cell Biology Department, Poznan University of Medical Sciences, Poznań, Poland.

Polyacrylamide gel electrophoresis, followed by an appropriate staining, is a popular and useful analytical procedure for protein identification and characterization. The aim of this study was to develop a method for protein visualization in polyacrylamide gels that would be alternative to Coomassie blue or silver staining. The proposed method is simple, fast and inexpensive. Read More

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Isolation and identification of guaiacol producing Alicyclobacillus fastidiosus strains from orchards in Poland.

Acta Biochim Pol 2021 May;68(2):301-307

Department of Microbiology, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology - State Research Institute, Warsaw, Poland.

The genus Alicyclobacillus comprises a group of Gram-positive, thermo-acidophilic bacteria that are capable of producing highly resistant endospores during unfavorable environmental conditions. The members of this genus inhabit natural environments, including hot springs and soils. The main reason behind the spoilage of final commercial fruit products by Alicyclobacillus is the contamination of fruits with soil at the time of harvesting. Read More

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miR-19a-3p inhibition alleviates sepsis-induced lung injury via enhancing USP13 expression.

Acta Biochim Pol 2021 May;68(2):201-206

Department of Intensive Care Unit (ICU), Nanjing First Hospital-Nanjing Medical University, Nanjing, Jiangsu Province, 210009, China.

Sepsis is a systemic inflammatory response syndrome caused by various pathogenic microorganisms or toxins. Lung damage is one of the causes of death in patients with sepsis. This study aimed to investigate the role of miR-19a-3p and its regulation mechanism in sepsis-induced lung injury. Read More

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The amelioration of T2DM rat femoral bone achieved by anti-osteoporosis of caprine CSN1S2 protein through bone morphogenetic protein signaling pathway.

Acta Biochim Pol 2021 May;68(2):265-275

Graduate School of Agriculture, Kyoto University, Kyoto, Japan.

We investigated the potential anti-glycation and anti-osteoporosis properties of Caprine milk CSN1S2 protein on the serum AGEs and sRAGE level, osteogenic factors expressions, femoral bone mesostructure, histomorphometry, and hydroxyapatite crystals changes in T2DM rats. Varying doses of Caprine milk CSN1S2 protein (0, 375, 750, and 1500 mg/kg BW) were used to treat the control and T2DM rats. We measured AGEs and sRAGE level; RUNX2, OSX, BMP2, and Caspase-3 expressions in rats using ELISA and immunohistochemistry, respectively. Read More

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Kidney graft function and arterial stiffness in renal transplant recipients.

Acta Biochim Pol 2021 May;68(2):331-339

Medizinische Klinik mit Schwerpunkt Nephrologie und Internistische Intensivmedizin Charité - Universitätsmedizin Berlin, Berlin, Germany.

Introduction: In renal transplant recipients (RTRs), cardiovascular (CV) complications are associated with non-traditional risk factors, such as a decline in graft function, immunosuppressive therapy, time of dialysis before transplantation, inflammation and anemia. Higher value of arterial stiffness is the consequence of risk factors and it can lead to CV events. The aim of this study was the assessment of the arterial stiffness in RTRs with different value of estimated glomerular filtration rate (eGFR) and its correlation with classical and non-classical CV risk factors. Read More

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Pseudomonas aeruginosa induces spatio-temporal secretion of IL-1β, TNFα, proMMP-9, and reduction of epithelial E-cadherin in human alveolar epithelial type II (A549) cells.

Acta Biochim Pol 2021 May;68(2):207-215

Department of Inmunobioquímica, Instituto Nacional de Perinatología "Isidro Espinoza de los Reyes" (INPerIER), Ciudad de México, México.

Pseudomonas aeruginosa, is an opportunistic bacterium with a high prevalence in diverse pulmonary infections. Although several genes are involved in the system of resistance and evasion of the immunological response of the host, little is known about the inflammatory, degradative, and cell-binding response induced by P. aeruginosa in human lung alveolar epithelial cells. Read More

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Lithium ions display weak interaction with amyloid-beta (Aβ) peptides and have minor effects on their aggregation.

Acta Biochim Pol 2021 Apr;68(2):169-179

Department of Biochemistry and Biophysics, Stockholm University, Sweden.

Alzheimer's disease (AD) is an incurable disease and the main cause of age-related dementia worldwide, despite decades of research. Treatment of AD with lithium (Li) has showed promising results, but the underlying mechanism is unclear. The pathological hallmark of AD brains is deposition of amyloid plaques, consisting mainly of amyloid-β (Aβ) peptides aggregated into amyloid fibrils. Read More

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Sequence variation analysis of the E1 and E2 genes of human papillomavirus type 16 in cervical lesions in women from the south of Poland.

Acta Biochim Pol 2021 Apr;68(2):341-346

Jagiellonian University Medical College, Faculty of Medicine, Chair of Microbiology, Department of Molecular Medical Microbiology, Kraków, Poland.

The E1 and E2 genes of the human papillomavirus encode the so-called early proteins, their sequences are conserved, and regulatory functions are associated with the viral oncoproteins. The purpose of this study is to determine the HPV16 E1 and E2 mutations appearing in the female population of southern Poland, depending on the severity of cervical pathological changes. We also take into account the number of E1 and E2 mutations detected in the E6 gene variant (350G or 350T). Read More

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A meta-analysis of the influence of the external conditions on the biosensor receptor layer component stability.

Acta Biochim Pol 2021 Apr;68(2):325-330

Department of Computer Science and Automatics, University of Bielsko-Biala, 43-309 Bielsko-Biala, Poland.

The manuscript presented here contains meta-analysis of the influence of the external conditions on the biosensor receptor layer component stability. The novelty of this paper is due to compilation and comparison of studies, based on proposed collective analyses. The presented meta-analysis allows to increase the precision and accuracy of the results by combining and co-analyzing data from five smaller experiments. Read More

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Clinical significance of cancer stem cell markers in lung carcinoma.

Acta Biochim Pol 2021 Apr;68(2):187-191

Department of Radiotherapy, The Second Affiliated Hospital of Nantong University, No.6 of Haierxiang North Road, Nantong 226001, Jiangsu, PR China.

The identification of side population (SP) cells in several cancer studies has been proved to be involved in the treatment failure (chemotherapy) and tumor relapse. Here we have sorted 7% of side population (SP) cells from lung adenocarcinoma by Hoechst 33342 dye expulsion method. Further, the characterization of sorted SP cells showed cancer stem like properties such as transcriptional upregulation of stemness genes (OCT-4, SOX2 and NANOG), ATP binding cassette (ABC) transporter protein (ABCG2) and enhanced level of stem cell surface markers such as CD133 and CD44. Read More

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Abnormal expression of FAK and paxillin correlates with oral cancer invasion and metastasis.

Acta Biochim Pol 2021 Apr;68(2):317-323

Department of Stomatology, Affiliated Hospital of Jilin Medical University, Jilin, 132013, China.

Globally, the tenth most common cancer is the oral squamous cell carcinoma (OSCC) and the treatment strategy for improving of OSCC patients survival rate still remains a challenging one. Aberrant regulation of cell to extracellular matrix protein interactions leads to progression of human cancers. The focal adhesion kinase (FAK) and its downstream target paxillin have been implicated in cancer growth, migration, invasion and metastasis of different cancers. Read More

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Evidence of independent introductions of RHDV2 strains in Poland based on the genome analysis of viral isolates from 2016-2018.

Acta Biochim Pol 2021 Apr;68(2):255-263

Department of Foot and Mouth Disease, National Veterinary Research Institute, Zduńska Wola, National Veterinary Research Institute, Puawy Poland.

The aim of this study was the molecular epidemiology of independently introduced RHDV2 strains in Poland. The nucleotide sequences of RHDV2 diagnosed in domestic rabbits in 2018 in the voivodeships of Swietokrzyskie (strain PIN), Malopolskie (strain LIB) and Mazowieckie (strain WAK), and RHDVa from 2015 (strain F77-3) recognized in wild rabbits in Kujawsko-Pomorskie voivodeship were compared to the genome sequences of the first native RHDV2 strains from 2016-2017. The reference sequences available in public databases, the representative for a classical RHDV (G1-G5 genogroups), RHDVa (G6), non-pathogenic caliciviruses (RCV, GI. Read More

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ARF3 inhibits proliferation and promotes apoptosis in gastric cancer by regulating AKT and ERK pathway.

Acta Biochim Pol 2021 Apr;68(2):223-229

Department of Digestive Tumor Surgery, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, 210029, Chin.

ADP-ribosylation factor 3 (ARF3) has confirmed participate in diverse biological processes in many cancers. However, the expression patterns and roles of ARF3 in gastric cancer (GC) remains largely unknown. In our study, by using qRT-PCR and western blot, we found that, in In GC tissues and cells, the expression of ARF3 was significantly down-regulated. Read More

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LncRNA UCA1 alleviates aberrant hippocampal neurogenesis through regulating miR-375/SFRP1-mediated WNT/β-catenin pathway in kainic acid-induced epilepsy.

Acta Biochim Pol 2021 Apr;68(2):159-167

Department of Graduate School, Guangxi University of Chinese Medicine, Nanning, Guangxi, 530023, China.

Temporal lobe epilepsy (TLE) is a chronic disease of the nervous system, associated with increased proliferation in the hippocampus. Urothcarcinoma associated 1 (UCA1) is a long long non-coding RNA that was shown to regulate proliferation and differentiation of neural progenitors in vitro. We hypothesised that TLE-associated abnormal proliferation is a consequence of the downregulation of UCA1. Read More

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Inhibition of GPR4 attenuates SH-SY5Y cell injury in cerebral ischemia/reperfusion via anti-apoptotic pathways.

Acta Biochim Pol 2021 Apr;68(2):181-186

Department of Neurology, The First Hospital of Zibo, Zibo City, Shandong Province, 255200, China.

Cerebral ischemia/reperfusion injury (CIRI) can lead to increased vascular endothelial permeability and blood-brain barrier damage in patients with stroke. G protein-coupled receptor 4 (GPR4) is a functional pH sensor that plays a key role in renal ischemia-reperfusion-induced apoptosis. However, whether GPR4 has a role in cerebral ischemia remains to be further studied. Read More

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miREIA - an immunoassay method in assessment of microRNA levels in tumor tissue-pilot study. The impact of miR-93-5p, miR-142-5p and IFNγ on PD-L1 level in colorectal cancer.

Acta Biochim Pol 2021 Apr;68(2):247-254

Department of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 19 Jordana, 41-800 Zabrze, Poland.

Colorectal cancer is the second and third most common cancer in females and males, respectively. The PD-L1/PD-1 immune checkpoint is an important source of immunosuppression in the tumor microenvironment and is associated with IFNγ. Recent studies have revealed that a significant number of tumor suppressive miRNAs can regulate the expression of PD-L1. Read More

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MiR-490 alleviates sepsis-induced acute lung injury by targeting MRP4 in new-born mice.

Acta Biochim Pol 2021 Mar;68(2):151-158

Department of Paediatrics, Ruian People's Hospital, Ruian Ciy, Wenzhou City, Zhejiang Province, 325200, China.

The aim of this study was to investigate whether the effects of miR-490 on acute lung injury (ALI) induced by sepsis in vitro and in vivo were through targeting multi-drug resistance-associated protein 4 (MRP4). MiR-490 agomir/NC agomir was injected into mice before cecal ligation and puncture (CLP). Pulmonary microvascular endothelial cells (PMVECs) were transfected with or without miR-490 agomir/NC agomir/MRP4/empty vector before lipopolysaccharide (LPS) stimulation. Read More

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MiR-1179 is downregulated in cervical cancer and its overexpression suppresses cancer cells invasion by targeting CHAF1A/ZEB1.

Acta Biochim Pol 2021 Mar;68(2):193-199

Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, 710004, China.

The anticancer effect of miR-1179 has been extensively studied in many tumors. The mechanism of miR-1179 action in cervical cancer, however, remains largely unknown. In the present study, miR-1179 was downregulated in both cervical cancer cell lines and cancer tissues. Read More

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Naturally occurring aesculetin coumarin exerts antiproliferative effects in gastric cancer cells mediated via apoptotic cell death, cell cycle arrest and targeting PI3K/AKT/M-TOR signalling pathway.

Acta Biochim Pol 2021 Mar;68(1):109-113

1Department of General Surgery, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing, China; 2Department of General Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

Aesculetin is an active member of coumarins that has been reported to possess significant medicinal and biological importance. It has also been shown with potential anticancer activity against different human cancers including breast, lung and hepatocellular carcinoma. Therefore, the current investigation was undertaken to examine the anticancer effects of aesculetin against gastric cancer. Read More

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