4,163 results match your criteria Acta Biochimica Polonica[Journal]


Cis-2 and trans-2-eisocenoic fatty acids are novel inhibitors for Mycobacterium tuberculosis Protein tyrosine phosphatase A.

Acta Biochim Pol 2020 Jun;67(2):219-223

Department of Chemistry, Universitas Muhammadiyah Riau, Indonesia.

Small protein tyrosine phosphatase (PtpA) of Mycobacterium tuberculosis is attributed to the development of latent tuberculosis infection, and hence bocomes an interesting target for drug development. In this communication, inhibition of PtpA by naturally occurring fatty acids cis-2 and trans-2-eicosenoic acid is investigated. Mtb PtpA was heterologously expressed in Escherichia coli, and the activity of PtpA was inhibited by cis-2 and trans-2 eicosenoic fatty acids. Read More

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http://dx.doi.org/10.18388/abp.2020_5201DOI Listing

Neogambogic acid induces apoptosis of melanoma B16 cells via the PI3K/Akt/mTOR signaling pathway.

Acta Biochim Pol 2020 Jun 19;67(2):197-202. Epub 2020 Jun 19.

The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, 550001, China.

Background: Neogambogic acid, as one of the main components of gamboge, exhibits high activities against various tumors.

Objective: To explore the mechanism by which melanoma B16 cell apoptosis was induced by neogambogic acid.

Methods: Melanoma B16 cells were treated with different concentrations of neogambogic acid solutions (0, 1. Read More

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http://dx.doi.org/10.18388/abp.2020_5188DOI Listing

Long non-coding RNA LINC00662 promotes proliferation and migration of breast cancer cells via regulating the miR-497-5p/EglN2 axis.

Acta Biochim Pol 2020 Jun;67(2):229-237

Department of Pathology, The First Affiliated Hospital of Wannan Medical College, Wuhu City, Anhui Province, 241001, China.

Previous reports indicated that long noncoding RNA 662 (LINC00662) plays a crucial role in several human cancers. Here, we studied the expression pattern of LINC00662 and explored its function in human breast cancer. The expression level of LINC00662 was determined in human breast cancer cell lines and tissues by real-time quantitative polymerase chain reaction (RT-qPCR). Read More

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http://dx.doi.org/10.18388/abp.2020_5203DOI Listing

Changes in daily life reduce indoor exposure to selected endocrine disruptors in the home environment: a pilot intervention study.

Acta Biochim Pol 2020 Jun;67(2):273-276

Department of Analytical Chemistry, Faculty of Chemistry, Gdansk University of Technology, Gdansk, Poland.

Increasing prevalence of lifestyle diseases raised global awareness about health consequences of human exposure to endocrine disruptors (EDs): synthetic chemicals that mimic natural hormones and affect the biochemical and endocrine balance. As home environment is one of the main sources of the exposure to xenobiotics - especially for pregnant women, infants and young children - health organizations emphasize the need of implementing lifestyle changes to protect human health and child development. The aim of this study was to evaluate the effectiveness of introducing changes in daily life in lowering the exposure to selected EDs in the indoor home environment. Read More

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http://dx.doi.org/10.18388/abp.2020_5369DOI Listing

Ultrasound-assisted transdermal delivery of alendronate for the treatment of osteoporosis.

Acta Biochim Pol 2020 Jun;67(2):173-179

Department of Traumatic Orthopaedics,Taian City Central Hospital,No.29,Longtan Road,Taian,Shandong Province,271000,China.

The aim of the current research work was to develop sonophoresis-assisted transdermal patches for the treatment of osteoporosis. In the present investigation, we formulated alendronate-chitosan nanoparticles by ionotropic external gelation method. The prepared nanoparticles were found to be smooth and free-flowing. Read More

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http://dx.doi.org/10.18388/abp.2020_5162DOI Listing

Vitamin D and disease activity in rheumatoid arthritis patients: a retrospective study in a Romanian cohort.

Acta Biochim Pol 2020 Jun;67(2):267-272

University of Medicine and Pharmacy "Victor Babeș", Timișoara, Romania.

Background: The relationship between the serum levels of Vitamin D and the severity of RA is a subject of great interest for the future therapeutic strategies. Although the evidence on the relationship between hypovitaminosis D and early RA is contradictory, preliminary data suggest that the serum levels of vitamin D are inversely associated with the disease activity.

Aim: the main objectives of this study include: (1) to analyze the serum levels of vitamin D in patients with RA in comparison to healthy controls; (2) to investigatea possible correlation with disease activity. Read More

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http://dx.doi.org/10.18388/abp.2020_5360DOI Listing

Oxypaeoniflorin improves myocardial ischemia/reperfusion injury by activating the Sirt1/Foxo1 signaling pathway.

Authors:
Kai Wang Wei Hu

Acta Biochim Pol 2020 Jun;67(2):239-245

Department of TCM Pharmacy,University of Chinese Academy of Sciences, Ningbo Huamei Hospital, China.

Myocardial ischemia/reperfusion (MI/R) injury is a leading cause of damage to cardiac tissues and is associated with high mortality and disability rates worldwide. Oxypaeoniflorin (OPA) has been found to be the main constituent of Paeonia veitchii Lynch. This study was conducted to explore the effect of OPA on MI/R injury and its potential mechanism. Read More

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http://dx.doi.org/10.18388/abp.2020_5206DOI Listing

SRPX2 promotes cell proliferation and invasion via activating FAK/SRC/ERK pathway in non-small cell lung cancer.

Acta Biochim Pol 2020 Jun;67(2):165-172

Departmen of Geriatrics, Wuhan NO. 1 Hospital, Wuhan, Hubei, China.

Background: Recent studies showed that sushi repeat containing protein X linked 2 (SRPX2) could participate in the development of various malignant tumors. However, its role in non-small cell lung cancer (NSCLC) was unknown. The aim of the study was to prospectively investigate the role of SRPX2 in NSCLC cell proliferation, migration and invasion and reveal the underlying mechanism. Read More

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http://dx.doi.org/10.18388/abp.2020_5158DOI Listing

Excessive amount and activity of μ-calpain affects apoptotic machinery in chronic B-cell leukemia cells and influences the course of the disease.

Acta Biochim Pol 2020 Jun;67(2):247-257

Gdańsk Medical University, Gdańsk, Poland.

B-cell Chronic Lymphocytic Leukemia (B-CLL) is the most common hematological disorder among middle-aged/elderly people in the Western countries. We have shown earlier that B-CLL cells exhibit elevated total amount and available activity of µ-calpain, belonging to a family of ubiquitous, strongly Ca-dependent proteases, involved in the control of proliferation and apoptosis. In this study we attempted to estimate a potential clinical value of μ-calpain in relation to B-CLL clinical staging in patients with extremely high lymphocytosis and studied the molecular mechanisms associating calpain activity with clinical progress of the disease. Read More

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http://dx.doi.org/10.18388/abp.2020_5226DOI Listing

Coincidence of 3-methylglutaconic aciduria and duplication 5q - a case report and literature review.

Acta Biochim Pol 2020 Jun;67(2):263-266

Department of Clinical Genetics, Medical College, University of Rzeszów, Rzeszów, Poland.

3-methylglutaconic aciduria includes a heterogeneous group of inborn errors of metabolism. The disease may have various clinical presentations, as can duplication 5q. We present the case of a 13-year-old boy with 3-methylglutaconic aciduria and duplication 5q. Read More

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http://dx.doi.org/10.18388/abp.2020_5355DOI Listing

Comparison and evaluation of three different molecular methods for detection of human Betapapillomaviruses in skin biopsies from patients with nonmelanoma skin cancer and precancerous lesions.

Acta Biochim Pol 2020 Jun;67(2):189-195

1Jagiellonian University Medical College, Faculty of Medicine, Chair of Microbiology, Department of Virology, Kraków, Poland.

Betapapillomaviruses have been linked to the development of nonmelanoma skin cancers. A great diversity of these viruses in skin specimens requires the use of sensitive and reliable detection methods. There are currently no standardized assays for diagnostic purposes. Read More

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http://dx.doi.org/10.18388/abp.2020_5185DOI Listing

Preclinical study of Doxorubicine-loaded liposomal drug delivery for the treatment of head and neck cancer: Optimization by Box-Behnken statistical design.

Authors:
Baihui Yang

Acta Biochim Pol 2020 Jun;67(2):149-155

Department of Cardiovascular Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650101, China.

The present investigation aimed at developing Doxorubicin (DOX)-loaded liposome-mediated drug delivery system for head and neck cancer. The liposomes were prepared by film hydration technique using egg phosphatidylcholine and cholesterol using Box-Behnken statistical design. The prepared liposomes were evaluated for the percentage encapsulation efficiency, particle size and in vitro release. Read More

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http://dx.doi.org/10.18388/abp.2020_5142DOI Listing

Neonatal cholestasis due to citrin deficiency: diagnostic pitfalls.

Acta Biochim Pol 2020 May;67(2):225-228

Department of Gastroenterology, Hepatology, Feeding Disorders and Pediatrics, The Children's Memorial Health Institute, Warsaw, Poland.

Citrin deficiency can manifest in newborns or infants as neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD). The paper presents a case of Polish NICCD patient presenting with low birth weight, failure to thrive, prolonged cholestatic jaundice with coagulopathy and hypoalbuminemia with normal results of MS/MS newborn screening but with high blood citrulline level observed at 3 months of age. Unreported findings included N-hypoglycosylation and increased serum very-long-chain fatty acids (VLCFA), probably secondary to liver impairment. Read More

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http://dx.doi.org/10.18388/abp.2020_5202DOI Listing

Antioxidative action of polyamines in protection of phospholipid membranes exposed to ozone stress.

Acta Biochim Pol 2020 May;67(2):259-262

Department of Biochemistry and Biophysics, Institute of Biology, Pedagogical University of Cracow, Kraków, Poland.

In the present work, Langmuir monolayers were used to study the interaction of putrescine (a cationic antioxidant) with anionic charged membranes (1,2-dioleoyl-sn-glycerol-3-phosphate) under oxidative stress caused by the presence of ozone in the water phase. Calcium ions and acidic environment were used to compare the electrostatic and antioxidant effects of putrescine with those of an inorganic cation. It has been shown that the main role of putrescine in protecting systems against oxidation is its rapid reaction with ROS. Read More

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http://dx.doi.org/10.18388/abp.2020_5230DOI Listing

Artemisinin is highly soluble in polyethylene Glycol 4000 and such solution has multiple biological effects.

Acta Biochim Pol 2020 May;67(2):203-211

1State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China; 2Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, College of Biotechnology, Southwest University, Chongqing, Chin.

Artemisinin has a significant role in treatment of malaria, as well as effective anti-inflammatory and anti-cancer activities. However, such problems as poor water solubility and easy recrystallization limit its application. In this study, polyethylene glycol, a solvent which is widely used in pharmaceutics, was introduced to prepare an artemisinin dissolution. Read More

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http://dx.doi.org/10.18388/abp.2020_5190DOI Listing

Selected CDKN2A and MDM2 polymorphisms in oral cavity cancer.

Acta Biochim Pol 2020 May;67(2):213-218

Department of Otorhinolaryngology and Oncological Laryngology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, Zabrze, Poland.

The head and neck squamous cell carcinoma (HNSCC) is an aggressive human malignancy diagnosed in more than 800 000 new cases worldwide, and mostly arises in the oral cavity, oropharynx, larynx, hypopharynx, and nasopharynx. The study presented here aimed to determine a possible association of rs11515 and rs3088440 gene polymorphisms in the CDKN2A gene (cyclin dependent kinase inhibitor 2A), as well as rs769412 and rs937283 in the MDM2 gene (murine double minute 2), with oral cavity cancer in a sample of Polish population; CDKN2A is crucial in regulating the cell cycle while MDM2 is an oncogene involved in regulating cell proliferation and apoptosis. The study included 95 primary tumor samples following surgical resection from patients, and the control group consisted of 100 healthy individuals. Read More

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http://dx.doi.org/10.18388/abp.2020_5195DOI Listing
May 2020
1.153 Impact Factor

Naringin induces endoplasmic reticulum stress-mediated apoptosis, inhibits β-catenin pathway and arrests cell cycle in cervical cancer cells.

Acta Biochim Pol 2020 Apr;67(2):181-188

Department of Gynaecology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian Province, 362000, China.

Naringin is a promising anticancer bioflavonoid phytochemical, mainly extracted from citrus fruits. This study evaluates the antiproliferative effect and the cell death mechanism induced by naringin on cervical cancer (CC) cells. Our results demonstrated that naringin exerts significant inhibition in cell viability and exhibits IC50 value 745, 764, 793 µM against C33A, SiHa, and HeLa cells respectively. Read More

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http://dx.doi.org/10.18388/abp.2020_5182DOI Listing

Intracellular mechanisms of tumor cells' immunoresistance.

Acta Biochim Pol 2020 Apr;67(2):143-148

Department of Pharmacodynamics, Medical University of Bialystok, Białystok, Poland.

One of the main mechanisms for avoiding immune response by cancer cells is mediated by inducing an immunosuppressive environment in the tumor following activation of immune checkpoints, i.e. PD-1 or CTLA-4 receptor inhibitors on T lymphocytes. Read More

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http://dx.doi.org/10.18388/abp.2020_2878DOI Listing

MiR-98-5p expression inhibits polarization of macrophages to an M2 phenotype by targeting Trib1 in inflammatory bowel disease.

Acta Biochim Pol 2020 Apr;67(2):157-163

Department of Anorectal Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 200021, China.

Herein, we unfolded miR-98-5p mechanism in inflammatory bowel disease (IBD). IBD mouse model was established. The severity of colitis was assessed daily using the disease activity index (DAI). Read More

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http://dx.doi.org/10.18388/abp.2020_5152DOI Listing
April 2020
1.153 Impact Factor

Prognostic value of Golgi P 73 and Beta 2-microglobulin in patients with Viral Hepatitis B.

Acta Biochim Pol 2020 Mar;67(1):7-14

Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, University of Dicle, Diyarbakir, Turkey.

Previous studies detected higher Golgi protein 73 levels in the serum of patients with chronic liver disease. The Beta-2 microglobulin levels were also observed to be higher in patients with chronic hepatitis B infection compared to the inactive carriers and the protein plays an important role in the response to viral infections. The aim of the present study was to assess the liver fibrosis through non-invasive methods in chronic hepatitis B patients. Read More

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http://dx.doi.org/10.18388/abp.2020_5209DOI Listing

The metabolic disorders associated with chronic consumption of soft and energy drinks in rats.

Acta Biochim Pol 2020 Mar;67(1):79-84

1Department of Pharmacology and Toxicology (subdivision of Biochemistry), College of Pharmacy, Taibah University, Medina, Kingdom of Saudi Arabia; 2Department of Biochemistry, Faculty of Pharmacy, Minia University, Minia, Egypt.

Background: Energy Drinks (EDs) and Soft Drinks (SDs) are widely consumed among adolescents and young adults. These drinks contain variable amounts of caffeine which is a central nervous system stimulator; in addition to sugar, taurine, vitamins and herbal extracts. Several adverse effects have been reported for the excessive consumption of caffeine and sugar. Read More

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http://dx.doi.org/10.18388/abp.2020_2914DOI Listing

Effect of maternal dietary supplementation with phytosterol esters on muscle development of broiler offspring.

Acta Biochim Pol 2020 Mar;67(1):135-141

Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou 510640, Guangdong, People's Republic of China.

Recently, embryo muscle development, which is crucial for postnatal skeletal muscle growth, has been investigated widely. Nutrients in ovo were suggested to be critical in embryo muscle development since the chick growth mostly relies on nutrients in eggs at the early developmental stage. Phytosterol esters (PE), which are derived from the reactions between phytosterols and fatty acids, were demonstrated to have important effects on lipid and cholesterol metabolism regulation. Read More

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http://dx.doi.org/10.18388/abp.2020_2884DOI Listing
March 2020
1.153 Impact Factor

Analytical ultracentrifugation as a tool in the studies of aggregation of the fluorescent marker, Enhanced Green Fluorescent Protein.

Acta Biochim Pol 2020 Mar;67(1):85-91

Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Warsaw, Poland.

Enhanced green fluorescent protein (EGFP) is a fluorescent marker used in bio-imaging applications, including as an indicator of folding or aggregation of a fused partner. However, the limited maturation, low folding efficiency, and presence of non-fluorescent states of EGFP can influence the interpretation of experimental data. To measure aggregation associated with de novo folding of EGFP from a high GdnHCl concentration, the analytical ultracentrifugation method was used. Read More

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http://dx.doi.org/10.18388/abp.2020_5081DOI Listing

A crystallization and preliminary X-ray diffraction study of the Arabidopsis thaliana proliferating cell nuclear antigen (PCNA2) alone and in a complex with a PIP-box peptide from Flap endonuclease 1.

Acta Biochim Pol 2020 Mar;67(1):49-52

Faculty of Life and Environmental Sciences, University of Yamanashi, 4-4-37 Takeda, Kofu, Yamanashi 400-8510, Japan.

DNA replication is an important event for all living organisms and the mechanism is essentially conserved from archaea, bacteria to eukaryotes. Proliferating cell nuclear antigen (PCNA) acts as the universal platform for many DNA transacting proteins. Flap endonuclease 1 (FEN1) is one such enzyme whose activity is largely affected by the interaction with PCNA. Read More

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http://dx.doi.org/10.18388/abp.2020_2896DOI Listing

P2X7 receptor activity landscape in rat and human glioma cell lines.

Acta Biochim Pol 2020 Mar;67(1):7-14

Laboratory of Molecular Basis of Cell Motility, Nencki Institute of Experimental Biology of Polish Academy of Sciences, Warsaw, Poland.

P2X7 is a commonly expressed purinergic receptor, which functions as a cation-permeable channel in the plasma membrane. In certain circumstances, the receptor may also form a large transmembrane pore what results in cell death. P2X7 receptors control numerous physiological and pathological cellular processes and their overexpression is often associated with cancer progression. Read More

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http://dx.doi.org/10.18388/abp.2020_2848DOI Listing

Roundup-induced biochemical and histopathological changes in the liver and kidney of rats: the ameliorative effects of Linum usitatissimum oil.

Acta Biochim Pol 2020 Mar;67(1):53-64

Laboratory of Biochemistry and Environmental Toxicology, Faculty of Sciences, University of Badji Mokhtar, Annaba, Algeria.

The present study was undertaken to evaluate the protective effects of Linum usitatissimum oil (LuO) against sub-chronic Roundup (RDP)-induced toxicity and oxidative stress in rats. Rats were divided into four groups: control group (no treatment), RDP group (Roundup at 269.9 mg/kg b. Read More

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http://dx.doi.org/10.18388/abp.2020_2898DOI Listing
March 2020
1.153 Impact Factor

Dose-dependent production of linoleic acid analogues in food derived Lactobacillus plantarum K25 and in silico characterization of relevant reactions.

Acta Biochim Pol 2020 Mar;67(1):123-129

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing.

The objective of this study was to assess and scrutinize the competency of probiotic L. plantarum K25 to produce linoleic acid analogues in the medium supplemented with different concentrations of linoleic acid, ranging from 1% to 10%, in a dose dependent manner. The analogues produced were identified and quantitated by GC-MS and in silico studies were done to confirm enzymatic reactions involved in its conversion. Read More

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http://dx.doi.org/10.18388/abp.2020_5167DOI Listing
March 2020
1.153 Impact Factor

Hemagglutination inhibition antibody titers as a correlate of protection against influenza disease in the 2018/2019 epidemic season in Poland.

Acta Biochim Pol 2020 Mar;67(1):93-98

Department of Influenza Research - National Influenza Center, National Institute of Public Health - National Institute of Hygiene, Warsaw, Poland.

The aim of this study was to determine the level of antibodies against hemagglutinin of influenza viruses in the sera of people in the seven age groups in the epidemic season 2018/2019 in Poland. The level of anti-hemagglutinin antibodies was determined by hemagglutination inhibition test (HAI). 1050 clinical samples from all over the country were tested. Read More

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http://dx.doi.org/10.18388/abp.2020_5088DOI Listing

Downregulation of long non-coding RNA ZXF1 restricts cell survival by targeting miR-634-GRB2 in lung adenocarcinoma.

Acta Biochim Pol 2020 Mar;67(1):31-39

Department of Respiratory and Critical Care Medicine, Renmin Hospital of Wuhan University, Wuhan City, Hubei Province, 430061, China.

Increasing evidence demonstrates that long non-coding RNAs (lncRNAs) play important regulatory roles in mediating initiation and progression of lung adenocarcinoma (LA), which is one of the most lethal in humans. A previous study reported that lncRNAZXF1 was dysregulated in LA and enhanced expression of ZXF1 promoted the invasion and metastasis in LA. However, the effect of ZXF1 on LA progression and its underlying mechanisms were not thoroughly investigated. Read More

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http://dx.doi.org/10.18388/abp.2020_2875DOI Listing

Can VEGFR-3 be a better tumor marker for breast cancer than CA 15-3?

Acta Biochim Pol 2020 Mar;67(1):25-29

Department of Population Medicine and Civilization Diseases Prevention, Medical University of Bialystok, Białystok, Poland.

Vascular Endothelial Growth Factor Receptor 3 (VEGFR-3) is a very important factor which promotes lymphangiogenesis not only in physiological but also in pathological processes in which we can include neoplasia. The aim of this study was to analyze the plasma concentrations and diagnostic utility of this parameter in comparison and in combination with CA 15-3 in breast cancer (BC) patients and in relation to the control groups. The study included 120 breast cancer and 60 control patients (28 with benign breast tumors and 32 healthy women). Read More

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http://dx.doi.org/10.18388/abp.2020_2873DOI Listing

Genetic variability and phylogenetic analysis of Lagovirus europaeus strains GI.1 (RHDV) and GI.2 (RHDV2) based on the RNA-dependent RNA polymerase (RdRp) coding gene.

Acta Biochim Pol 2020 Mar;67(1):111-122

Institute of Biology, University of Szczecin, Szczecin, Poland; Molecular Biology and Biotechnology Center, University of Szczecin, Szczecin, Poland.

Lagovirus europaeus GI.1 (RHDV-rabbit haemorrhagic disease virus) and GI.2 (RHDV2-rabbit haemorrhagic disease virus 2), family Caliciviridae, genus Lagovirus, are etiological factors of the rabbit haemorrhagic disease (RHD). Read More

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http://dx.doi.org/10.18388/abp.2020_5161DOI Listing

The effect of luteolin 7-glucoside, apigenin 7-glucoside and Succisa pratensis extracts on NF-κB activation and α-amylase activity in HepG2 cells.

Acta Biochim Pol 2020 Mar;67(1):41-47

Department of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, Święcickiego 4, 60-781 Poznań, Poland.

The chemical composition of Succisa pratensis is not well known. The existing data indicate a substantial content of flavonoids, which include luteolin and apigenin 7-glucosides. The aim of this study was to elaborate the isolation protocol of these flavonoids from flowers and leaves of S. Read More

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http://dx.doi.org/10.18388/abp.2020_2894DOI Listing

The effect of D380Y pathogenic mutation in human Yin Yang 1 on the protein's structure and function.

Acta Biochim Pol 2020 Feb;67(1):73-77

Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.

Yin Yang 1 is a human transcription factor that controls a number of genes and takes part in the regulation of cell cycle, proliferation, differentiation, and neuronal development. Yin Yang 1 is composed of an N-terminal intrinsically disordered fragment and a C-terminal domain responsible for binding to DNA, composed of four zinc fingers. Recently, various alterations in the Yin Yang 1's DNA binding domain were linked with an unexplained intellectual disability named Gabriele-de Vries syndrome. Read More

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http://dx.doi.org/10.18388/abp.2020_2911DOI Listing
February 2020

Vitamin D in autoimmune bullous disease.

Authors:
Stefan Tukaj

Acta Biochim Pol 2020 Feb;67(1):1-5

Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland.

Numerous epidemiological studies have suggested a link between vitamin D deficiency and the development of various autoimmune diseases, including diabetes mellitus type 1, rheumatoid arthritis, inflammatory bowel disease, multiple sclerosis or systemic lupus erythematosus. More recently, such a link has been also proposed for autoimmune bullous diseases (AIBD). This is a relatively rare and potentially life-threatening, organ-specific group of inflammatory skin diseases characterized by the presence of tissue-bound and circulating autoantibodies against various molecules present in desmosomes (in pemphigus diseases) or hemidesmosomes (in pemphigoid diseases). Read More

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http://dx.doi.org/10.18388/abp.2020_2905DOI Listing
February 2020

In silico characterization of linoleic acid biotransformation to rumenic acid in food derived Lactobacillus plantarum YW11.

Acta Biochim Pol 2020 Feb;67(1):99-109

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing, China.

Lactobacillus plantarum YW11 capability to convert linoleic acid into conjugated linoleic acid and other metabolites was studied in a dose-dependent manner by supplementing LA at different concentrations. L. plantarum YW11 displayed a uniform distinctive growth curve of CLA and other metabolites at concentrations of LA ranging from 1% (w/v) to 10% (w/v), with slightly increased growth at higher LA concentrations. Read More

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http://dx.doi.org/10.18388/abp.2020_5095DOI Listing
February 2020
1.153 Impact Factor

Olanzapine-mediated cardiotoxicity is associated with altered energy metabolism in isolated rat hearts.

Acta Biochim Pol 2020 Jan;67(1):15-23

Institute of Clinical and Translational Research, Biomedical Research Center, University Science Park for Biomedicine, Slovak Academy of Sciences, Bratislava, Slovak Republic.

Olanzapine is an antipsychotic drug routinely used for the treatment of schizophrenia. Although the olanzapine treatment is associated with disturbed electrical heart activity, the exact mechanism underlying this severe adverse effect remains unclear. Recently, olanzapine administration was demonstrated to be associated with elevation of blood glucose and lower levels of free fatty acids. Read More

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http://dx.doi.org/10.18388/abp.2020_2871DOI Listing
January 2020

The effects of clofarabine in ALL inhibition through DNA methylation regulation.

Acta Biochim Pol 2020 Jan;67(1):65-72

Department of Biomedical Chemistry, Faculty of Health Sciences, Medical University of Lodz, Łódź, Poland.

Clofarabine (2-chloro-2'-fluoro-2'-deoxyarabinosyladenine, ClF), a second-generation 2'-deoxyadenosine analog, possesses manifold anti-cancer activities. Our previous reports and some of others demonstrate the potential capacity of ClF to regulate the epigenetic machinery. The study presented here is the first to investigate the influence of ClF on modulators of the DNA methylation machinery, including DNMT1 and CDKN1A, in acute lymphoblastic leukemia (ALL) cells. Read More

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http://dx.doi.org/10.18388/abp.2020_2901DOI Listing
January 2020

Hydrogen sulfide formation in experimental model of acute pancreatitis.

Acta Biochim Pol 2019 Dec;66(4):611-618

Chair of Medical Biochemistry, Jagiellonian University, Collegium Modicum, Kraków, Poland.

Acute pancreatitis (AP) is a disease defined as acute or chronic inflammatory process of the pancreas characterized by premature activation of digestive enzymes within the pancreatic acinar cells and causing pancreatic auto-digestion. In mammalian tissues, H2S is synthesized endogenously from L-cysteine in regulated enzymatic pathways catalyzed by pyridoxal phosphate-dependent enzymes: cystathionine beta - synthase (CBS), gamma - cystathionase (CTH) and cysteine aminotransferase (CAT) coupled with 3-mercaptopyruvate sulfurtransferase (MPST). In the mitochondria, hydrogen sulfide is oxidized to sulfite, which is then converted to thiosulfate (sulfane sulfur-containing compound) by thiosulfate sulfurtransferase (rhodanese; TST). Read More

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http://dx.doi.org/10.18388/abp.2019_2900DOI Listing
December 2019

Impact of developmental origin, niche mechanics and oxygen availability on osteogenic differentiation capacity of mesenchymal stem/stromal cells.

Acta Biochim Pol 2019 Dec;66(4):491-498

Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.

Mesenchymal Stem/Stromal Cells (MSCs) have been widely considered as a promising source of cells for tissue regeneration. Among other stem cells, they are characterized by a high osteogenic potential. Intensive studies in this field had shown that even if basic osteogenic differentiation is relatively simple, its clinical application requires more sophisticated approaches to prepare effective and safe cell therapy products. Read More

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http://dx.doi.org/10.18388/abp.2019_2893DOI Listing
December 2019

UV radiation in HCT 116 cells influences intracellular H2O2 and glutathione levels, antioxidant expression, and protein glutathionylation.

Acta Biochim Pol 2019 Dec;66(4):605-610

1System Engineering Group, Institute of Automatic Control, Silesian University of Technology, Gliwice, Poland; 2Biotechnology Centre, Silesian University of Technology, Gliwice, Poland.

UV radiation influences cellular levels of hydrogen peroxide (H2O2) and glutathione (GSH) and alters the expression of antioxidant genes in the human colorectal cancer cell line HCT116. In this study, cells were irradiated with UV light of different wavelength (A, B, or C). A surge in H2O2 concentration and total glutathione (level occurred 6 hours later. Read More

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http://dx.doi.org/10.18388/abp.2019_2892DOI Listing
December 2019
1.153 Impact Factor

Nanoparticles as energy donors and acceptors in bionanohybrid systems.

Acta Biochim Pol 2019 Dec;66(4):469-481

Department of Biophysics, Faculty of Biotechnology, University of Wroclaw, Wrocław, Poland.

The bionanohybrids are the junctions of at least two objects of different origin: abiotic and biotic. The abiotic part is a nanoparticle (often a fluorescent quantum dot), the biotical one may be a protein (especially fluorescent one or redox-active one), nucleic acid, carbohydrate as well as a simple organic molecule. When such a junction undergoes illumination, the energy transfer between the partners is possible. Read More

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http://dx.doi.org/10.18388/abp.2019_2887DOI Listing
December 2019

Secretion, migration and adhesion as key processes in the therapeutic activity of mesenchymal stem cells.

Acta Biochim Pol 2019 Dec;66(4):499-507

Jagiellonian University, Medical College, Department of Transplantation, Kraków, Poland.

The MSCs are immature cells that can be found in numerous different tissue types. In recent years, they have gained considerable attention, particularly with regard to their regenerative properties. Due to their paracrine activity, ability to migrate, adhesion and homing, MSCs currently appear to be the most relevant for therapeutic use. Read More

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http://dx.doi.org/10.18388/abp.2019_2895DOI Listing
December 2019

Molecular mechanisms of epithelial to mesenchymal transition in tumor metastasis.

Acta Biochim Pol 2019 Dec;66(4):509-520

Jagiellonian University Medical College, Faculty of Medicine, Institute of Pediatrics, Department of Transplantation, Kraków, Poland.

Epithelial to mesenchymal transition (EMT) is a process during which cancer cells lose epithelial features, cytoskeletal architecture is re-organized, cell shape changes and cells activate genes that help to define mesenchymal phenotype, what leads to an increased cell motility and dissemination of tumor to distant metastatic sites. This review describes different signaling networks between microRNAs and proteins that regulate EMT in tumor growth. Activation of EMT is mediated via series of paracrine signaling molecules. Read More

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http://dx.doi.org/10.18388/abp.2019_2899DOI Listing
December 2019

An outline of the use of supramolecular compounds in biology and medicine.

Authors:
Anna Jagusiak

Acta Biochim Pol 2019 Dec;66(4):545-549

1Chair of Medical Biochemistry, Faculty of Medicine, Jagiellonian University Medical College, Kraków, Poland.

Supramolecular chemistry opened up opportunities and prospects in biological studies. Numerous supramolecular systems are known which assume different shapes depending on the structure of the component molecules. Some of them are mixed systems. Read More

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http://dx.doi.org/10.18388/abp.2019_2910DOI Listing
December 2019

Sulfane sulfur - new findings on an old topic.

Acta Biochim Pol 2019 Dec;66(4):533-544

Chair of Medical Biochemistry, Jagiellonian University Medical College, Kraków, Poland.

Sulfane sulfur is a divalent sulfur atom bonded to another sulfur which is very reactive and labile. Compounds containing this reactive sulfur include persulfides, polysulfides, thiosulfate, thiosulfinates, polythionates, and elemental sulfur. Sulfane sulfur appears in a number of biologically important compounds, including thiocysteine, thiocystine and thiotaurine, products of the cysteine metabolism, as well as glutathione persulfide. Read More

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http://dx.doi.org/10.18388/abp.2019_2909DOI Listing
December 2019

Is the mechanism of nitroglycerin tolerance associated with aldehyde dehydrogenase activity? A contribution to the ongoing discussion.

Acta Biochim Pol 2019 Dec;66(4):627-632

Chair of Medical Biochemistry, Jagiellonian University, Medical College, Kraków, Poland.

The aim of the study presented here was an attempt to answer the question posed in the title: Is the mechanism of nitroglycerin tolerance associated with aldehyde dehydrogenase (ALDH) activity? Here, we investigated the effect of administration (separately or jointly) of lipoic acid (LA), nitroglycerin (GTN), and disulfiram (DSF; an irreversible in vivo inhibitor of all ALDH isozymes (including ALDH2)), on the development of tolerance to GTN. We also assessed the total activity of ALDH in the rat liver homogenates. Our data revealed that not only DSF and GTN inhibited the total ALDH activity in the rat liver, but LA also proved to be an inhibitor of this enzyme. Read More

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http://dx.doi.org/10.18388/abp.2019_2908DOI Listing
December 2019

E-cadherin, Snail, ZEB-1, DNMT1, DNMT3A and DNMT3B expression in normal and breast cancer tissues.

Acta Biochim Pol 2019 Dec;66(4):409-414

Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Objective: Breast cancer is known as one of very important cancers among females, given that a variety of external (i.e., environmental risk factors) and internal factors (i. Read More

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http://dx.doi.org/10.18388/abp.2019_2808DOI Listing
December 2019

Conformational flexibility and ligand binding properties of ovine β-lactoglobulin.

Acta Biochim Pol 2019 Dec;66(4):577-584

Department of Crystal Chemistry and Crystal Physics, Faculty of Chemistry, Jagiellonian University, Kraków, Poland.

Ovine β‑lactoglobulin was characterized by spectroscopic (CD), calorimetric (ITC) and X-ray structural studies. The structure of ovine β‑lactoglobulin complex with decanol showed that tight packing of molecules in the crystalline phase enforces a distortion of protein flexible loops resulting in the formation of an asymmetric dimer. The loops surrounding β-barrel in ovine lactoglobulin possessed the same conformational flexibility as observed previously in other lactoglobulins and the change of their conformation regulates the access to the binding pocket. Read More

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http://dx.doi.org/10.18388/abp.2019_2883DOI Listing
December 2019

Novel combinatorial transdermal drug delivery of alendronate with risedronate for the treatment of osteoporosis.

Acta Biochim Pol 2019 Dec;66(4):463-467

Department of Orthopedics, Shidong Hospital, Yangpu District, Shanghai, 200438, China.

The presented investigation explores the efficiency of novel transdermal drug delivery system of combination of two drugs i.e risedronate and alendronate in the treatment of osteoporosis. The nanoparticulate transdermal patch was prepared using PLGA as principle polymer which has been found to be suitable for drug delivery. Read More

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http://dx.doi.org/10.18388/abp.2019_2863DOI Listing
December 2019

Cladonia uncialis as a valuable raw material of biosynthetic compounds against clinical strains of bacteria and fungi.

Acta Biochim Pol 2019 Dec;66(4):597-603

Department of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, Poznań, Poland.

Cladonia uncialis is a lichen species with confirmed antibacterial activity and whose genome has been recently sequenced, enabling first attempts in its functional characterization. In this work, we investigated activity of the C. uncialis acetone extract (CUE) and usnic acid (UA) enantiomers against ten clinical microbial strains causing skin infections. Read More

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http://dx.doi.org/10.18388/abp.2019_2891DOI Listing
December 2019