3,651 results match your criteria Acta Biochimica Et Biophysica Sinica[Journal]


DDR1 promotes LoVo cell proliferation by regulating energy metabolism.

Acta Biochim Biophys Sin (Shanghai) 2022 Mar 25. Epub 2022 Mar 25.

Department of Gastroenterology, The Second Hospital of Lanzhou University, Lanzhou 730030, China.

Cellular energy metabolism dysregulation is associated with colorectal cancer (CRC) development and progression. Discoidin domain receptor1a (DDR1a), one of the five DDR1 isoforms, is closely related to cell proliferation, invasion, and apoptosis in various tumors. Whether it participates in cellular metabolic reprogramming and regulates CRC initiation and progression remains unclear. Read More

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CDKN2AIP-induced cell senescence and apoptosis of testicular seminoma is associated with CARM1 and eIF4β.

Acta Biochim Biophys Sin (Shanghai) 2022 Apr 25. Epub 2022 Apr 25.

Department of Gynaecology and Obstetrics, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.

Testicular seminoma is a relatively rare tumor which is mostly detected in male population aged from 15 to old. Although several molecular biomarkers have been identified to be associated with testicular seminoma pathogenesis, the exact mechanism for testicular seminoma progression remains largely unknown. CDKN2AIP (CDKN2A interacting protein) has previously been identified as a tumor suppressor in multiple malignant diseases. Read More

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CORO1C, a novel PAK4 binding protein, recruits phospho-PAK4 serine 99 to the leading edge and promotes the migration of gastric cancer cells.

Acta Biochim Biophys Sin (Shanghai) 2022 Apr 25. Epub 2022 Apr 25.

Gastric cancer is one of the malignant tumors in the world. PAK4 plays an important role in the occurrence and development of gastric cancer, especially in the process of invasion and metastasis. Here we discover that CORO1C, a member of coronin family that regulates microfilament and lamellipodia formation, recruits cytoplasmic PAK4 to the leading edge of gastric cancer cells by C-terminal extension (CE) domain of CORO1C The localization of PAK4 on the leading edge of the cell depends on two necessary conditions: the phosphorylation of PAK4 on serine 99 and the binding to the CE domain of CORO1C. Read More

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Translesion synthesis of apurinic/apyrimidic site analogues by Y-family DNA polymerase Dbh from.

Acta Biochim Biophys Sin (Shanghai) 2022 Apr 25. Epub 2022 Apr 25.

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

Apurinic/apyrimidic (AP) sites are severe DNA damages and strongly block DNA extension by major DNA polymerases. Y-family DNA polymerases possess a strong ability to bypass AP sites and continue the DNA synthesis reaction, which is called translesion synthesis (TLS) activity. To investigate the effect of the molecular structure of the AP site on the TLS efficiency of Dbh, a Y-family DNA polymerase from , a series of different AP site analogues (various spacers) are used to characterize the bypass efficiency. Read More

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Alternative end-joining in BCR gene rearrangements and translocations.

Acta Biochim Biophys Sin (Shanghai) 2022 May 25. Epub 2022 May 25.

Department of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.

Programmed DNA double-strand breaks (DSBs) occur during antigen receptor gene recombination, namely V(D)J recombination in developing B lymphocytes and class switch recombination (CSR) in mature B cells. Repair of these DSBs by classical end-joining (c-NHEJ) enables the generation of diverse BCR repertoires for efficient humoral immunity. Deletion of or mutation in c-NHEJ genes in mice and humans confer various degrees of primary immune deficiency and predisposition to lymphoid malignancies that often harbor oncogenic chromosomal translocations. Read More

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Positive allosteric regulation of PAC1-R up-regulatesPAC1-R and its specific ligand PACAP.

Acta Biochim Biophys Sin (Shanghai) 2022 Apr 25. Epub 2022 Apr 25.

Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou 510630, China.

PAC1-R is a recognized preferential receptor for the neuropeptide of pituitary adenylate cyclase-activating polypeptide (PACAP), which mediates neuroprotective and nerve regenerative activities of PACAP. In this study, we found that in both PAC1R-CHO cells with high expression of PAC1R-eGFP and retinal ganglion cells (RGC-5) with the natural expression of PAC1-R, oligo-peptide PACAP(28-38) and the positively charged arginine-rich penetrating peptide TAT, as positive allosteric modulators of PAC1-R, significantly trigger the nuclear translocation of PAC1-R. The chromatin immunoprecipitation (ChIP)-PCR results show that the nuclear translocated PAC1-R binds with the promoter regions of and its specific ligand . Read More

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Osteoblasts induce glucose-derived ATP perturbations in chondrocytes through noncontact communication.

Acta Biochim Biophys Sin (Shanghai) 2022 Apr 25. Epub 2022 Apr 25.

State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.

Cartilage and subchondral bone communicate with each other through material and signal exchanges. However, direct evidence provided by experimental studies on their interactions is insufficient. In the present study, we establish a noncontact co-culture model with a transwell chamber to explore the energetic perturbations in chondrocytes influenced by osteoblasts. Read More

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Gene, virulence and related regulatory mechanisms in Cryptococcus gattii.

Acta Biochim Biophys Sin (Shanghai) 2022 Mar 25. Epub 2022 Mar 25.

Department of Respiratory and Critical Care, Beijing Shijitan Hospital, Capital Medical University; Peking University Ninth School of Clinical Medicine, Beijing 100089, China.

is a kind of basidiomycetous yeast, which grows in human and animal hosts. . has four distinct genomes, VGI/AFLP4, VGII/AFLP6, VGIII/AFLP5, and VGIV/AFLP7. Read More

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Computational investigations on target-site searching and recognition mechanisms by thymine DNA glycosylase during DNA repair process.

Acta Biochim Biophys Sin (Shanghai) 2022 May 25. Epub 2022 May 25.

Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China.

DNA glycosylase, as one member of DNA repair machineries, plays an essential role in correcting mismatched/damaged DNA nucleotides by cleaving the N-glycosidic bond between the sugar and target nucleobase through the base excision repair (BER) pathways. Efficient corrections of these DNA lesions are critical for maintaining genome integrity and preventing premature aging and cancers. The target-site searching/recognition mechanisms and the subsequent conformational dynamics of DNA glycosylase, however, remain challenging to be characterized using experimental techniques. Read More

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DNA damage and repair in the hematopoietic system.

Acta Biochim Biophys Sin (Shanghai) 2022 Jan 25. Epub 2022 Jan 25.

Department of Medical Genetics and Molecular Diagnostic Laboratory, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China.

Although hematopoietic stem cells (HSCs) in the bone marrow are in a state of quiescence, they harbor the self-renewal capacity and the pluripotency to differentiate into mature blood cells when needed, which is key to maintain hematopoietic homeostasis. Importantly, HSCs are characterized by their long lifespan (.. Read More

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January 2022

Ginsenoside Rh2 inhibits breast cancer cell growth viaERβ-TNFα pathway.

Acta Biochim Biophys Sin (Shanghai) 2022 Apr 25. Epub 2022 Apr 25.

Department of Biochemistry and Molecular Biology, Hunan Province Key Laboratory of Basic and Applied Hematology & Hunan Key Laboratory of Animal Models for Human Diseases, School of Life Sciences, Central South University, Changsha 410008, China.

Ginsenoside Rh2 is one of rare panaxidiols extracted from Panax ginseng and a potential estrogen receptor ligand that exhibits moderate estrogenic activity. However, the effect of Rh2 on growth inhibition and its underlying molecular mechanism in human breast cells are not fully understood. In this study, we tested cell viability by MTT and colony formation assays. Read More

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CPT1C promotes the potential of gastric cancer ovarian metastasis through up-regulating fatty acid oxidation.

Acta Biochim Biophys Sin (Shanghai) 2022 Feb 25. Epub 2022 Feb 25.

Department of General Surgery, Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

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February 2022

NEDD4L inhibits cell viability, cell cycle progression, and glutamine metabolism in esophageal squamous cell carcinoma via ubiquitination of c-Myc.

Acta Biochim Biophys Sin (Shanghai) 2022 May 25. Epub 2022 May 25.

Department of Hematologic and Oncology, Xinjiang Clinical Research Center for Precision Medicine of Digestive System Tumor, the Center Hospital of Karamay City, Karamay 834000, China.

Esophageal squamous cell carcinoma (ESCC) is a common subtype of esophageal cancer with high incidence. Surgery remains the main strategy for treatment of ESCC at early stage. However, the treatment outcome is unsatisfactory. Read More

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Four temporin-derived peptides exhibit antimicrobial and antibiofilm activities against methicillin-resistant.

Acta Biochim Biophys Sin (Shanghai) 2022 Mar;54(3):350-360

Key Laboratory of Tropical Biological Resources of Ministry of Education, School of Pharmaceutical Sciences, Hainan University, Haikou 570228, China.

Temporin-GHa (GHa) was cloned from , showing a weak antimicrobial activity. In order to improve its bactericidal efficacy, GHaR6R, GHaR7R, GHaR8R and GHaR9W were designed and synthesized. Compared to the parent peptide, the GHa-derived peptides show potent antimicrobial activities against methicillin-resistant (MRSA), which is the main pathogen with high morbidity and mortality that causes various infections in humans. Read More

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LncRNA H19 inhibits oxidative stress injury of cochlear hair cells by regulating miR-653-5p/SIRT1 axis.

Acta Biochim Biophys Sin (Shanghai) 2022 Mar;54(3):332-339

Department of Otolaryngology, Head and Neck Surgery, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, China.

Oxidative stress is one of the important mechanisms of inner ear cell damage, which can lead to age-related hearing loss (ARHL). LncRNA H19 is significantly downregulated in the cochlea of old mouse, however, the role of H19 in the development of ARHL remains unclear. In this study, we aim to investigate the expression and function of H19 in oxidative stress injury of cochlear hair cells induced by HO. Read More

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Taraxasterol ameliorates dextran sodium sulfate-induced murine colitis via improving intestinal barrier and modulating gut microbiota dysbiosis.

Acta Biochim Biophys Sin (Shanghai) 2022 Mar;54(3):340-349

Department of Gastroenterology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

Taraxasterol (TAX) has been proven to prevent and treat inflammatory diseases. However, the effects of TAX on intestinal barrier and the diversity, structure, and function of gut microbiota have yet to be elucidated in dextran sodium sulfate (DSS)-induced colitis mice. Our objectives are to evaluate the effect of TAX on intestinal barrier and its impact on gut microbiota. Read More

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Evaluation of the predominant bacteria and proinflammatorycytokine expressions in odontogenic cysts.

Acta Biochim Biophys Sin (Shanghai) 2022 03;54(3):409-411

National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology&Shanghai Research Institute of Stomatology, Department of Oral Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, China.

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Functions of non-coding RNAs in regulating cancer drug targets.

Acta Biochim Biophys Sin (Shanghai) 2022 Mar;54(3):279-291

With the development of precision medicine, the efficiency of tumor treatment has been significantly improved. More attention has been paid to targeted therapy and immunotherapy as the key to precision treatment of cancer. Targeting epidermal growth factor receptor (EGFR) has become one of the most important targeted treatments for various cancers. Read More

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Micropeptides translated from putative long non-coding RNAs.

Acta Biochim Biophys Sin (Shanghai) 2022 Mar;54(3):292-300

MOE Laboratory of Biosystem Homeostasis and Protection, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.

Long non-coding RNAs (lncRNAs) transcribed in mammals and eukaryotes were thought to have no protein coding capability. However, recent studies have suggested that plenty of lncRNAs are mis-annotated and virtually contain coding sequences which are translated into functional peptides by ribosomal machinery, and these functional peptides are called micropeptides or small peptides. Here we review the rapidly advancing field of micropeptides translated from putative lncRNAs, describe the strategies for their identification, and elucidate their critical roles in many fundamental biological processes. Read More

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Curcumin ameliorates HO-induced injury through SIRT1-PERK-CHOP pathway in pancreatic beta cells.

Acta Biochim Biophys Sin (Shanghai) 2022 Mar;54(3):370-377

Department of Biochemistry and Molecular Biology, Hengyang Medical School, University of South China, Hengyang 421001, China.

Oxidative stress and endoplasmic reticulum (ER) stress play crucial roles in pancreatic β cell destruction, leading to the development and progression of type 1 diabetes mellitus (T1DM). Curcumin, extracted from plant turmeric, possesses multiple bioactivities such as antioxidant, anti-inflammatory and anti-apoptosis properties and . However, it remains unknown whether curcumin improves ER stress to prevent β cells from apoptosis. Read More

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LINC00891 regulated by miR-128-3p/GATA2 axis impedes lung cancer cell proliferation, invasion and EMT by inhibiting RhoA pathway.

Acta Biochim Biophys Sin (Shanghai) 2022 Mar;54(3):378-387

Cancer Center and Research Institute, University of Mississippi Medical Center, Guyton Research Building, G-651-07, 2500 North State Street, Jackson, MS 39216, USA.

Long non-coding RNA (lncRNA) LINC00891 knockdown is associated with poor prognosis of lung adenocarcinoma, but the underlying mechanism remains to be further explored. Here, we found that LINC00891 expression is downregulated in lung cancer tissues and cell lines compared with that in adjacent normal tissues and normal lung epithelial cells. LINC00891 overexpression impedes cell proliferation, invasion, migration and epithelial-to-mesenchymal transition (EMT) process in lung cancer cells. Read More

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Endoplasmic reticulum stress mediates homocysteine-induced hypertrophy of cardiac cells through activation of cyclic nucleotide phosphodiesterase 1C.

Acta Biochim Biophys Sin (Shanghai) 2022 Mar;54(3):388-399

Center for Basic Medical Research & Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300457, China.

Although the association of elevated homocysteine level with cardiac hypertrophy has been reported, the molecular mechanisms by which homocysteine induces cardiac hypertrophy remain inadequately understood. In this study we aim to uncover the roles of cyclic nucleotide phosphodiesterase 1 (PDE1) and endoplasmic reticulum (ER) stress and their relationship to advance the mechanistic understanding of homocysteine-induced cardiac cell hypertrophy. H9c2 cells and primary neonatal rat cardiomyocytes are exposed to homocysteine with or without ER stress inhibitor TUDCA or PDE1-specific inhibitor Lu AF58027, or transfected with siRNAs targeting PDE1 isoforms prior to homocysteine-exposure. Read More

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CD82 palmitoylation site mutations at Cys5+Cys74 affect EGFR internalization and metabolism through recycling pathway.

Acta Biochim Biophys Sin (Shanghai) 2022 Mar;54(3):400-408

Department of Clinical Laboratory, the Second Affiliated Hospital of Dalian Medical University, Dalian 116023, China.

Tetraspanin CD82 often participates in regulating the function of epidermal growth factor receptor (EGFR) and hepatocyte growth factor receptor (c-Met). Palmitoylation is a post-translational modification that contributes to tetraspanin web formation and affects tetraspanin-dependent cell signaling. However, the molecular mechanisms by which CD82 palmitoylation affects the localization and stability of EGFR and c-Met have not yet been elucidated. Read More

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Inhibition of USP7 suppresses advanced glycation end-induced cell cycle arrest and senescence of human umbilical vein endothelial cells through ubiquitination of p53.

Acta Biochim Biophys Sin (Shanghai) 2022 Mar;54(3):311-320

Diabetes mellitus is a n arising public health concern, and diabetic foot is one of the most common complications of diabetes. Current management for diabetic foot cannot reach optimal remission. In this study, we aim to explore the mechanism underlying the pathogenesis of diabetic foot and provide novel strategies for the treatment of diabetic foot. Read More

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TGF-β1-induced RAP2 regulates invasion in pancreatic cancer.

Acta Biochim Biophys Sin (Shanghai) 2022 Mar;54(3):361-369

Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai 200032, China.

Pancreatic cancer is highly lethal due to its aggressive invasive properties and capacity for metastatic dissemination. Additional therapeutic targets and effective treatment options for patients with tumours of high invasive capacity are required. Ras-related protein-2a (RAP2) is a member of the GTP-binding proteins. Read More

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Down-regulation of lncRNA LUADT1 suppresses cervical cancer cell growth by sequestering microRNA-1207-5p.

Acta Biochim Biophys Sin (Shanghai) 2022 Mar;54(3):321-331

Emerging evidence has proved the essential roles of long non-coding RNAs (lncRNAs) in cervical carcinoma (CC). LncRNA lung adenocarcinoma-associated transcript 1 (LUADT1) is overexpressed and plays an oncogenic role in various cancers; however, the function and clinical values of LUADT1 in CC remain unclear. In this study we found that LUADT1 is highly expressed in CC tissues and cells. Read More

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Mitochondrial fission factor promotes cisplatin resistancein hepatocellular carcinoma.

Acta Biochim Biophys Sin (Shanghai) 2022 Mar;54(3):301-310

Department of Burns and Cutaneous Surgery, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, China.

Hepatocellular carcinoma (HCC) is the most common primary liver tumor and one of the leading causes of cancer-related death worldwide. Chemotherapeutic agents/regimens such as cisplatin (DDP) are frequently used for advanced HCC treatment. However, drug resistance remains a major hindrance and the underline mechanisms are not fully understood. Read More

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RP5-1148A21.3 (lncRP5) exerts oncogenic function in human ovarian carcinoma.

Acta Biochim Biophys Sin (Shanghai) 2022 Feb;54(2):209-219

Ovarian cancer (OC) is a fatal gynecological malignancy that is difficult to diagnose at early stages. Various long non-coding RNAs (lncRNAs) are aberrantly expressed in OC and exert regulatory effects on OC; however, the underlying mechanism requires in-depth investigation. This work is designed to explore the molecular regulatory axis of a newly identified lncRNA in OC, that is, lncRNA RP5-1148A21. Read More

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February 2022

Hypoxia-induced RBBP7 promotes esophagus cancer progression by inducing CDK4 expression.

Acta Biochim Biophys Sin (Shanghai) 2022 Feb;54(2):179-186

Hypoxia-induced epigenetic regulation calls for more effective therapeutic targets for esophageal cancer. We used GEPIA and UALCAN databases to screen survival-related and cancer stage-associated genes. Eca109 and KYSE450 esophageal cancer cell lines were cultured under normoxia, hypoxia, or CoCl-induced hypoxia conditions, which were further transfected with plasmids expressing RB binding protein 7 (RBBP7), hypoxia-inducible factor 1 (HIF1)-α, or RBBP7 shRNA. Read More

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February 2022