1,258 results match your criteria Acs Sensors[Journal]


Organic field-effect transistor platform for label-free single-molecule detection of genomic biomarkers.

ACS Sens 2020 Jun 4. Epub 2020 Jun 4.

The rising interest for technologies capable to track a biomarker down to the physical limit points towards new opportunities in early diagnostics of progressive diseases. Indeed, single molecule detection technologies are foreseen to enable clinicians to associate a biomarker tiniest increase to the progression of a disease, particularly at its early stage. Bioelectronic organic transistors represent an extremely powerful tool to achieve label-free and single-molecule detection of clinically relevant biomarkers. Read More

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http://dx.doi.org/10.1021/acssensors.0c00694DOI Listing

Design of a histidine kinase FRET sensor to detect complex signal integration within living bacteria.

ACS Sens 2020 Jun 4. Epub 2020 Jun 4.

Histidine kinases switch between conformational states that promote kinase and phosphatase activi-ties to regulate diverse cellular processes. Past stud-ies have shown these functional states can display heterogeneity between cells in microbial communi-ties and can vary at the subcellular level. Methods to track and correlate kinase conformational state with the phenotypic response of living bacteria cells will offer new opportunities to interrogate bacterial signaling mechanisms. Read More

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http://dx.doi.org/10.1021/acssensors.0c00008DOI Listing

Aerosol-to-hydrosol sampling and simultaneous enrichment of airborne bacteria for rapid biosensing.

ACS Sens 2020 Jun 4. Epub 2020 Jun 4.

Rapid monitoring of biological particulate matter (Bio-PM, bioaerosols) requires an enrichment technique for concentrating the Bio-PM dispersed in air into a small volume of liquid. In this study, an electrostatic air sampler is employed to capture aerosolized test bacteria in a carrier liquid (aerosol-to-hydrosol (ATH) enrichment). Simultaneously, the captured bacteria are carried into a fluid channel for hydrosol-to-hydrosol (HTH) enrichment with Concanavalin A-coated magnetic particles (CMPs). Read More

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http://dx.doi.org/10.1021/acssensors.0c00555DOI Listing

Selective Detection of Ethylene by MoS-Carbon Nanotube Networks Coated with Cu(I)-Pincer Complexes.

ACS Sens 2020 Jun 4. Epub 2020 Jun 4.

The plant hormone ethylene (C2) can induce premature fruit ripening and flower senescence at levels below 1 ppm, which has motivated efforts to develop cost-effective methods for C2 monitoring during the transport and storage of climacteric fruits. Here we describe a nanocomposite film composed of exfoliated MoS, single-walled carbon nanotubes (SCNTs), and Cu(I)-tris(mercaptoimidazolyl)borate complexes (Cu-Tm) for real-time detection of C2 at levels down to 100 ppb. A co-percolation network of MoS and SCNT was deposited onto interdigitated Ag electrodes printed on plastic substrates, then coated with Cu-Tm with final conductance in the 0. Read More

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http://dx.doi.org/10.1021/acssensors.0c00344DOI Listing

3-Aminophenyl boronic acid-functionalized quantum dot-based ratiometric fluorescence sensor for the highly sensitive detection of tyrosinase activity.

ACS Sens 2020 Jun 2. Epub 2020 Jun 2.

Using the commercially available and economical 6-hydroxycoumarin (6-HC) as the substrate, a dual-emission ratiometric fluorescence sensor was developed to detect tyrosinase (TYR) activity based on 3-aminophenyl boronic acid-functionalized quantum dots (APBA-QDs). TYR can catalyze 6-HC, a monohydroxy compound, to form a fluorescence-enhancing o-hydroxy compound, 6,7-dihydroxycoumarin. Owing to the special covalent binding between the o-hydroxyl and boric acid groups, APBA-QDs react with 6,7-dihydroxycoumarin to form a five-membered ring ester dual-emission fluorescence probe for TYR. Read More

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http://dx.doi.org/10.1021/acssensors.0c00122DOI Listing

Biosensors for Managing the COVID-19 Cytokine Storm: Challenges Ahead.

ACS Sens 2020 Jun 2. Epub 2020 Jun 2.

The global COVID-19 pandemic has oversaturated many intensive care units to the point of collapse, leading to enormous spikes in death counts. Before critical care becomes a necessity, identifying patients who are likely to become critically ill and providing prompt treatment is a strategy to avoid ICU oversaturation. There is a consensus that a hyperinflammatory syndrome or a "cytokine storm" is responsible for poor outcomes in COVID-19. Read More

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http://dx.doi.org/10.1021/acssensors.0c00979DOI Listing

Modulating the electrical properties of organic heterojunction devices based on phthalocyanines for ambipolar sensors.

ACS Sens 2020 Jun 1. Epub 2020 Jun 1.

Although ambipolar materials are highly studied in organic electronics, they are rarely used in gas sensors. In the present work, we studied ammonia sensing on organic heterojunctions in a bilayer configuration composed of oc-tachlorinated metallophthalocyanines (M(Cl8Pc); M: Co, Cu and Zn) as a sublayer and lutetium bis-phthalocyanine (LuPc2) as a top layer. Despite small effect of metal atom in M(Cl8Pc) on the device current and the interfacial energy barrier, a strong effect on NH3 sensing behavior was found such that Co(Cl8Pc), Cu(Cl8Pc) and Zn(Cl8Pc) based devices exhibited n-type, p-type and ambipolar charge carrier transport, respectively. Read More

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http://dx.doi.org/10.1021/acssensors.0c00877DOI Listing

A Sensor Array for the Nanomolar Detection of Azo Dyes in Water.

ACS Sens 2020 Jun 1. Epub 2020 Jun 1.

Azo dyes are ubiquitous pollutants that contaminate water supplies and threaten human, biota, and ecosystem health. Their detection and discrimination are a considerable challenge owing to the numerous structural, chemical, and optical similarities between dyes, complexity of the wastewater in which they are found, and low environmental concentrations. Here, we demonstrate that the inner filter effect (IFE), in combination with conjugated polymer array-based sensing, offers a rapid approach for the quantitative profiling of these pollutants. Read More

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http://dx.doi.org/10.1021/acssensors.0c00342DOI Listing

Nucleic Acid Quantitation with Log-Linear Response Hybridization Probe Sets.

ACS Sens 2020 Jun 1. Epub 2020 Jun 1.

Concentrations of different nucleic acid species in biological samples span many orders of magnitude. Real-time PCR maps the concentration of a target nucleic acid sequence log-linearly into cycle threshold to enable quantitation with a wide dynamic range, but suffers from enzymatic biases. Here, we present a general design for constructing hybridization probe sets with highly log-linear response curves to enable accurate enzyme-free quantitation across large ranges (more than 6 logs) of target DNA concentrations. Read More

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http://dx.doi.org/10.1021/acssensors.0c00052DOI Listing

Roll-to-roll manufacturing of integrated immunodetection sensors.

ACS Sens 2020 May 29. Epub 2020 May 29.

Lack of functional integration and high manufacturing costs have been identified as major challenges in commercialization of point-of-care devices. In this study, roll-to-roll (R2R) fabrication process was developed for large-scale manufacturing of disposable microfluidic devices. The integrated, user-friendly device included plasma separation membrane for simple blood filtration, immobilised antibodies for specific immunodetection, microfluidics for plasma transport and reagent mixing, and a blister for actuation and waste storage. Read More

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http://dx.doi.org/10.1021/acssensors.0c00404DOI Listing

Integrated Smart Janus Textile Bands for Self-Pumping Sweat Sampling and Analysis.

ACS Sens 2020 Jun 4. Epub 2020 Jun 4.

Research Center for Bioengineering and Sensing Technology, University of Science and Technology Beijing, 30 Xueyuan Road, Beijing 100083, P. R. China.

Wearable sweat sensors have spearheaded the thrust toward personalized health monitoring with continuous, real-time, and molecular-level insight in a noninvasive manner. However, effective sweat sampling still remains a huge challenge. Here, we introduce an intelligent Janus textile band that bridges the gap between self-pumping sweat collection, comfortable epidemic microclimate, and sensitive electrochemical biosensing via an integrated wearable platform. Read More

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http://dx.doi.org/10.1021/acssensors.0c00563DOI Listing

Dual-mode Detection of Bacterial 16S Ribosomal RNA in Tissues.

ACS Sens 2020 May 29. Epub 2020 May 29.

The specific detection of pathogens has long been recognized as a vital strategy for controlling bacterial infections. Herein, a novel hydrophilic aromatic-imide-based thermally activated delayed fluorescence (TADF) probe, , is designed and synthesized by conjugation of a TADF emitter with a bacterial 16S ribosomal RNA-targeted moiety, neomycin. Biological data showed for the first time that could be successfully applied for the dual-mode detection of bacterial 16S rRNA using confocal fluorescence imaging and time-resolved fluorescence imaging (TRFI) in both cells and tissues. Read More

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http://dx.doi.org/10.1021/acssensors.0c00252DOI Listing

Machine-learning for Precision Breast Cancer Diagnosis and Prediction of the Nanoparticles Cellular internalization.

ACS Sens 2020 May 28. Epub 2020 May 28.

In the field of theranostics, diagnostic nanoparticles are de-signed to collect highly patient-selective disease profiles, which is then leveraged by a set of nanotherapeutics to improve the therapeutic results. Despite their early promise, high inter-patient and intra-tumoral heterogeneities make any rational design and analysis of these theranostics platforms remained extremely problematic. Recent advances in deep learning-based tools may help bridge this gap, using pattern recognition algo-rithms for better diagnostic precision and therapeutic outcome. Read More

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http://dx.doi.org/10.1021/acssensors.0c00329DOI Listing

Imaging single bacterial cells with electro-optical impedance microscopy.

ACS Sens 2020 May 26. Epub 2020 May 26.

Impedance measurements have been an important tool for biosensor applications, including protein detection, DNA quantification and cell study. We present here an electro-optical impedance microscope (EIM) based on the dependence of surface optical transmission on local surface charge density for single bacteria impedance imaging. We applied a potential modulation to bacteria placed on an indium tin oxide coated slide, and simultaneously recorded a sequence of transmitted microcopy images. Read More

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http://dx.doi.org/10.1021/acssensors.0c00751DOI Listing

Tumor-Penetrating Hierarchically Structured Nanomarker for Imaging-Guided Urinary Monitoring of Cancer.

ACS Sens 2020 Jun 4. Epub 2020 Jun 4.

School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, P. R. China.

The capacity to diagnose cancer with the existing endogenous biomarkers remains limited because biomarkers usually act at the tumor site and are thus challenging to be detected directly from body fluids with high sensitivity and specificity, especially in the early stage of tumorigenesis. Here, we demonstrate an exogenous tumor-penetrating nanomarker composed of fluorescent nanoparticles conjugated with specific fluorescein-labeled peptides. The injectable nanomarkers perform four functions: they penetrate the tumor, target sites of cancer, cleave specific peptides by on-target protease, and drop off the labeled peptide into host urine for fluorescent detection. Read More

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http://dx.doi.org/10.1021/acssensors.9b02194DOI Listing

Paper-based Analytical Device for Real-time Monitoring of Egg Hatching in the Model Nematode Caenorhabditis elegans.

ACS Sens 2020 May 26. Epub 2020 May 26.

Caenorhabditis elegans (C. elegans) is an in vivo model known for its easy handling and maintenance and lack of associated ethical issues. The release of chitinase can be used to monitor the egg laying stage in C. Read More

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http://dx.doi.org/10.1021/acssensors.0c00412DOI Listing

Ratiometric Fluorescent Nanohybrid for Non-Invasive and Visual Monitoring of Sweat Glucose.

ACS Sens 2020 May 25. Epub 2020 May 25.

Non-invasive and visual monitoring of glucose is highly desirable for diabetes diagnostics and long-term home-based health management. Owing to the correlation of glucose level between blood and sweat, on-body sweat glucose detection provides potential for non-invasive healthcare but is highly challenging. Herein, we for the first time demonstrate a wearable skin pad based on ratiometric fluorescent nanohybrid, which can realize non-invasive and visual monitoring of sweat glucose. Read More

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http://dx.doi.org/10.1021/acssensors.0c00718DOI Listing

Single-entity approach to investigate surface charge enhancement in magnetoelectric nanoparticles induced by AC magnetic field stimulation.

ACS Sens 2020 May 24. Epub 2020 May 24.

Magneto-electric nanoparticles (MENPs), composed of a piezoelectric shell and a ferromagnetic core, exhibited enhanced cell-uptake and controlled drug release due to the enhanced localized electric field (surface charge/potential) and the generation of acoustics, respectively, on applying alternating current (AC)-magnetic (B)-field stimulation. This research, for the first time, implements an electrochemical single-entity approach to probe AC B-field induced strain mediated surface potential enhancement on MENP surface. The surface potential changes at the single-NP level can be probed by the open circuit potential changes of the floating carbon nanoelectrode (CNE) during the MENP-CNE collision events. Read More

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http://dx.doi.org/10.1021/acssensors.0c00664DOI Listing

Efficient NH detection based on MOS sensors couple with a catalytic conversion.

ACS Sens 2020 May 24. Epub 2020 May 24.

A solution of ammonia (NH) detection based on a catalytic conversion of NH into NO was proposed by using metal oxide semiconductor (MOS) gas detectors and Platinum (Pt) supported catalysts. The catalysts convert NH into NO, which is a very sensitive analyte for MOS detectors. Catalysts based on Pt-loaded HZSM-5 and AlO (Pt/HZSM-5 and Pt/AlO) were prepared by wet impregnation. Read More

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http://dx.doi.org/10.1021/acssensors.0c00836DOI Listing

Porous Graphene Oxide-Metal Ion Composite for Selective Sensing of Organophosphate Gases.

ACS Sens 2020 May 25. Epub 2020 May 25.

Organophosphates are used as agricultural pesticides, and also encountered as toxic nerve agents in chemical warfare. Accordingly, development of sensors for detection and monitoring organophosphate vapors is highly sought. We present a new capacitive gas sensor exhibiting remarkable specificity and sensitivity towards the organophosphate nerve gas simulants triethyl-phosphate (TEP), dimethyl methyl phosphate (DMMP), and the pesticide dichlorvos (DDVP). Read More

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http://dx.doi.org/10.1021/acssensors.9b02367DOI Listing

Visualization and Quantification of Electrochemical H2 Bubble Nucleation at Pt, Au and MoS2 Substrates.

ACS Sens 2020 May 24. Epub 2020 May 24.

Electrolytic gas evolution is a significant phenomenon in many electrochemical technologies from water splitting, chloralkali process to fuel cells. Although it is known that gas evolution may substantially affect the ohmic resistance and mass transfer, studies focusing on the electrochemistry of individual bubbles is critical but also challenging. Here we report an approach using scanning electrochemical cell microscopy (SECCM) with a single channel pipet to quantitatively study individual gas bubble nucleation on different electrode substrates, including conventional polycrystalline Pt and Au films, as well as the most interesting two-dimensional semiconductor MoS2. Read More

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http://dx.doi.org/10.1021/acssensors.0c00913DOI Listing

Dynamic Complex Emulsions as Amplifiers for On-Chip Photonic Cavity-Enhanced Resonators.

ACS Sens 2020 May 22. Epub 2020 May 22.

Department of Chemistry and Institute for Soldier Nanotechnology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.

Despite the recent emergence of microcavity resonators as label-free biological and chemical sensors, practical applications still require simple and robust methods to impart chemical selectivity and reduce the cost of fabrication. We introduce the use of hydrocarbon-in-fluorocarbon-in-water (HC/FC/W) double emulsions as a liquid top cladding that expands the versatility of optical resonators as chemical sensors. The all-liquid complex emulsions are tunable droplets that undergo dynamic and reversible morphological transformations in response to a change in the chemical environment (. Read More

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http://dx.doi.org/10.1021/acssensors.0c00399DOI Listing

Noncovalently Caged Firefly Luciferins Enable Amplifiable Bioluminescence Sensing of Hyaluronidase-1 Activity in Vivo.

ACS Sens 2020 Jun 2. Epub 2020 Jun 2.

Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Food Engineering, Changsha University of Science and Technology, Changsha 410114, P. R. China.

Hyaluronidase 1 (Hyal-1) is an important enzyme involved in intracellular hyaluronic acid (HA) catabolism for performing various physiological functions, and its aberrant level is closely associated with many malignant diseases. Bioluminescence imaging is advantageous for monitoring Hyal-1 activity in vivo, but it remains challenging to design an available probe for differentiating Hyal-1 from other isoforms by a traditional strategy that covalently masks the firefly luciferase substrate. Herein, we, for the first time, present a noncovalently caging approach to construct a Hyal-1-specific bioluminogenic nanosensor by entrapping d-luciferin (d-Luc) inside the cholesterylamine-modified HA (CHA) nanoassembly to inhibit the bioluminescence production. Read More

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http://dx.doi.org/10.1021/acssensors.0c00393DOI Listing

Thread-based bioluminescent sensor for detecting multiple antibodies in a single drop of whole blood.

ACS Sens 2020 May 22. Epub 2020 May 22.

Antibodies are important biomarkers in clinical diagnostics in addition to being increasingly used for therapeutic purposes. Although numerous methods for their detection and quantification exist, they predominantly require benchtop instruments operated by specialists. To enable the detection of antibodies at point-of-care (POC), the development of simple and rapid assay methods independent of laboratory equipment is of high relevance. Read More

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http://dx.doi.org/10.1021/acssensors.0c00564DOI Listing

Detect to Protect.

Authors:
Michael J Sailor

ACS Sens 2020 May;5(5):1247-1248

Department of Chemistry and Biochemistry, University of California, San Diego, California, United States.

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http://dx.doi.org/10.1021/acssensors.0c00920DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7262728PMC

Single-measurement multiplexed quantification of microRNAs from human tissue using catalytic hairpin assembly and Förster resonance energy transfer.

ACS Sens 2020 May 21. Epub 2020 May 21.

Absolute quantification of microRNAs (miRNA) or other nucleic acid biomarkers is an important requirement for molecular and clinical biosensing. Emerging technologies with beneficial features concerning simplicity and multiplexing present an attractive route for advancing diagnostic tools toward rapid and low-cost bioanalysis. However, the actual translation into the clinic by miRNA quantification in human samples is often missing. Read More

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http://dx.doi.org/10.1021/acssensors.0c00432DOI Listing

Molecular Magnetic Resonance Imaging of Nitric Oxide in Biological Systems.

ACS Sens 2020 Jun 2. Epub 2020 Jun 2.

Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue Rm. 16-561, Cambridge, Massachusetts 02139, United States.

Detection of nitric oxide (NO) in biological systems is challenging due to both physicochemical properties of NO and limitations of current imaging modalities and probes. Magnetic resonance imaging (MRI) could be applied for studying NO in living tissue with high spatiotemporal resolution, but there is still a need for chemical agents that effectively sensitize MRI to biological NO production. To develop a suitable probe, we studied the interactions between NO and a library of manganese complexes with various oxidation states and molecular structures. Read More

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http://dx.doi.org/10.1021/acssensors.0c00322DOI Listing

Effect of Crystal Defect on Gas Sensing Properties of CoO Nanoparticle.

ACS Sens 2020 May 21. Epub 2020 May 21.

Crystal growth controlled CoO nanoparticles were prepared to examine gas sensing properties. A cube-like, an irregular shaped, and three kinds of raspberry-type structures were observed by morphology analysis. The raspberry-type structures have an expanded lattice volume with large oxygen deficiency area and the cube-like structure has a contracted lattice volume as compared to the irregular shaped structure. Read More

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http://dx.doi.org/10.1021/acssensors.0c00290DOI Listing

Paper-Based Batteries as Conductivity Sensors for Single-Use Applications.

ACS Sens 2020 Jun 3. Epub 2020 Jun 3.

Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), C/del Til·lers, Campus Universitat Autònoma de Barcelona (UAB), Bellaterra, 08193 Barcelona, Spain.

We present a novel approach to measure ionic conductivity with a self-powered strategy. In particular, we propose the use of a paper-based battery as a sensor. The battery sensor unit consists of two electrodes placed side-by-side and covered by a piece of hydrophilic paper strip. Read More

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http://dx.doi.org/10.1021/acssensors.0c00405DOI Listing

Nicotine Monitoring with a Wearable Sweat Band.

ACS Sens 2020 May 28. Epub 2020 May 28.

Department of Electrical Engineering and Computer Sciences, University of California, Berkeley, California 94720, United States.

The tobacco epidemic is a public health threat that has taken a heavy toll of lives around the globe each year. Smoking affects both the smokers and those who are exposed to secondhand smoke, and careful tracking of exposure can be key to mitigating the potential hazards. For smokers, the variation of chemical compositions between commercial cigarettes has led to ambiguity in estimating the health risks, both for active smokers and others involuntarily exposed to tobacco smoke and byproducts. Read More

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http://dx.doi.org/10.1021/acssensors.0c00791DOI Listing

Surface Enhanced Raman Spectroscopy Based Biosensor with a Microneedle Array for Minimally Invasive Glucose Measurements.

ACS Sens 2020 May 29. Epub 2020 May 29.

School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457, Singapore.

To monitor blood glucose levels reliably, diabetic patients usually have to undergo frequent fingerstick tests to draw out fresh blood, which is painful and inconvenient with the potential risk of cross contamination especially when the lancet is reused or not properly sterilized. This work reports a novel surface-enhanced Raman spectroscopy (SERS) sensor for the intradermal detection of glucose based on a low-cost poly(methyl methacrylate) microneedle (PMMA MN) array. After incorporating 1-decanethiol (1-DT) onto the silver-coated array surface, the sensor was calibrated in the range of 0-20 mM in skin phantoms then tested for the quantification of glucose in a mouse model of streptozocin (STZ)-induced type I diabetes. Read More

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http://dx.doi.org/10.1021/acssensors.0c00444DOI Listing

Review of Gravimetric Sensing of Volatile Organic Compounds.

ACS Sens 2020 May 29. Epub 2020 May 29.

Department of Electrical Engineering, Columbia University, New York, New York 10027-6902, United States.

Volatile organic compounds (VOCs) are pervasive in the environment. Since the early 1980s, substantial work has examined the detection of these materials, as they can indicate environmental changes that can affect human health. VOCs and similar compounds present a very specific sensing problem in that they are not reactive and often nonpolar, so it is difficult to find materials that selectively bind or adsorb them. Read More

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http://dx.doi.org/10.1021/acssensors.0c00333DOI Listing

N-Terminal Derivatization-Assisted Identification of Individual Amino Acids Using a Biological Nanopore Sensor.

ACS Sens 2020 May 26. Epub 2020 May 26.

Biomedical Engineering Program, University of South Carolina, Columbia, South Carolina 29208, United States.

Nanopore technology has been employed as a powerful tool for DNA sequencing and analysis. To extend this method to peptide sequencing, a necessary step is to profile individual amino acids (AAs) through their nanopore stochastic signals, which remains a great challenge because of the low signal-to-noise ratio and unpredictable conformational changes of AAs during their translocation through nanopores. We showed that the combination of an N-terminal derivatization strategy of AAs with nanopore technology could lead to effective differentiation of AAs. Read More

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http://dx.doi.org/10.1021/acssensors.0c00345DOI Listing

Enhanced Electrochemical Impedance Spectroscopy Analysis of Microbial Biofilms on an Electrochemically Generated Graphene Interface.

ACS Sens 2020 May 21. Epub 2020 May 21.

National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China.

Biofilms can cause many bacterial diseases, such as dental disease. An detection of biofilms may help to screen antibiofilm drugs. An impedance measurement based on an Au electrode has been successfully used for real-time monitoring of animal and human cell growth. Read More

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http://dx.doi.org/10.1021/acssensors.0c00570DOI Listing

Development of a Conformational Histamine H Receptor Biosensor for the Synchronous Screening of Agonists and Inverse Agonists.

ACS Sens 2020 May 28. Epub 2020 May 28.

Institute of Pharmacology and Toxicology and Rudolf Virchow Center, University of Würzburg, Würzburg 97070, Germany.

The histamine H receptor (HR) represents a highly attractive drug target for the treatment of various central nervous system disorders, but the discovery of novel HR targeting compounds relies on the assessment of highly amplified intracellular signaling events that do not only reflect HR modulation and carry the risk of high false-positive and -negative screening rates. To address these limitations, we designed an intramolecular HR biosensor based on the principle of bioluminescence resonance energy transfer (BRET) that reports the receptor's real-time conformational dynamics and provides an advanced tool to screen for both HR agonists and inverse agonists in a live cell screening-compatible assay format. This conformational G-protein-coupled receptor (GPCR) sensor allowed us to characterize the pharmacological properties of known and new H receptor ligands with unprecedented accuracy. Read More

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http://dx.doi.org/10.1021/acssensors.0c00397DOI Listing

Cascade Reaction-Based Chemiresistive Array for Ethylene Sensing.

ACS Sens 2020 May 11;5(5):1405-1410. Epub 2020 May 11.

Interdisciplinary Research Center for Catalytic Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8565, Japan.

Chemiresistive sensors, which are based on semiconducting materials, offer real-time monitoring of environment. However, detection of nonpolar chemical substances is often challenging because of the weakness of the doping effect. Herein, we report a concept of combining a cascade reaction (CR) and a chemiresistive sensor array for sensitive and selective detection of a target analyte (herein, ethylene in air). Read More

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http://dx.doi.org/10.1021/acssensors.0c00194DOI Listing

Ultrahigh-Frequency Surface Acoustic Wave Sensors with Giant Mass-Loading Effects on Electrodes.

ACS Sens 2020 May 19. Epub 2020 May 19.

National Engineering Research Centre for High Efficiency Grinding, College of Mechanical and Vehicle Engineering, Hunan University, Changsha 410082, P.R. China.

Surface acoustic wave (SAW) devices are widely used for physical, chemical, and biological sensing applications, and their sensing mechanisms are generally based on frequency changes due to mass-loading effects at the acoustic wave propagation area between two interdigitated transducers (IDTs). In this paper, a new sensing mechanism has been proposed based on a significantly enhanced mass-loading effect generated directly on Au IDT electrodes, which enables significantly enhanced sensitivity, compared with that of conventional SAW devices. The fabricated ultrahigh-frequency SAW devices show a significant mass-loading effect on the electrodes. Read More

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http://dx.doi.org/10.1021/acssensors.0c00259DOI Listing

Detection of Circulating Tumor Cells in Breast Cancer Patients by Nanopore Sensing with Aptamer-Mediated Amplification.

ACS Sens 2020 May 26. Epub 2020 May 26.

Department of Laboratory Medicine, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu 610041, China.

Circulating tumor cells (CTCs) have been utilized in the diagnosis and prognosis of tumor. However, the CTC concentration is extremely low to be detected in peripheral blood. Many existing methods suffer from either expensive labeling or complex operation. Read More

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http://dx.doi.org/10.1021/acssensors.9b02537DOI Listing

Smart Surface-Enhanced Resonance Raman Scattering Nanoprobe for Monitoring Cellular Alkaline Phosphatase Activity during Osteogenic Differentiation.

ACS Sens 2020 May 19. Epub 2020 May 19.

State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, Changchun 130012, PR China.

High-efficiency induction of bone marrow mesenchymal stem cells (BMSCs) to osteogenic differentiation in vitro can help solve a series of bone diseases such as bone injury, fracture repair, and osteoporosis. In order to explore the optimal conditions for different chemical inducers to promote BMSCs differentiation and the possible differentiation mechanisms, we developed a smart nanoprobe that can achieve in situ alkaline phosphatase (ALP) activity detection during osteogenic differentiation in cells. The smart nanoprobe (Au@BCIP) was designed as the surface decoration of gold nanoparticles (AuNPs) with 5-bromo-4-chloro-3-indolyl phosphate (BCIP). Read More

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http://dx.doi.org/10.1021/acssensors.0c00428DOI Listing

Deflected Talbot-Mediated Overtone Spectroscopy in Near-Infrared as a Label-Free Sensor on a Chip.

ACS Sens 2020 May 26. Epub 2020 May 26.

School of Electrical and Computer Engineering, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel.

Rapid, sensitive, and reliable detection of aromatic amines, toxic manufacturing byproducts, has been previously achieved with molecular vibrations in the mid-infrared (Mid-IR) region. However, Mid-IR spectroscopic tools are hampered by a need to prepare the samples and the sensor cost. Here, we develop an affordable label-free sensor on a chip, operating in near-infrared (NIR) for ultrasensitive detection of absorption line signatures based on molecular vibrations overtones of the aromatic amine -methylaniline probe molecule. Read More

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http://dx.doi.org/10.1021/acssensors.0c00325DOI Listing

A Methodology for Ultrasensitive Detection of Sequence-Specific DNA or Uracil-DNA Glycosylase Activity.

ACS Sens 2020 May 15. Epub 2020 May 15.

Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400044, PR China.

Ultrasensitive detection of sequence-specific DNA and uracil-DNA glycosylase (UDG) activity shows great practical significance in clinical diagnostic and biomedical studies. Here, a methodology based on a CRISPR/Cas12a system coupled with enhanced strand displacement amplification (E-SDA) was innovatively established for sequence-specific DNA or UDG activity detection. Sequence-specific DNA or DNA primers processed by UDG and Endonuclease IV can initiate E-SDA, generating auxiliary DNA chains, which act as activators to unlock the indiscriminate collateral cleavage activities (trans-cleavage) of the CRISPR/Cas12a. Read More

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http://dx.doi.org/10.1021/acssensors.0c00081DOI Listing

Modulating the Linker Immobilization Density on Aptameric Graphene Field Effect Transistors Using an Electric Field.

ACS Sens 2020 May 18. Epub 2020 May 18.

Key Laboratory of Micro-systems and Micro-structures Manufacturing, Harbin Institute of Technology, Ministry of Education, Harbin 150080, China.

Aptameric graphene-based field-effect transistors (A-GFETs) always employ linkers, which could immobilize on graphene through π-π stacking between contained pyrenyl groups and graphene, to anchor aptamers. Aptamer density is closely associated with the A-GFET sensitivity and determined by the linker density. Using known linker immobilization methods, the linker density is random, uncontrollable, and limited. Read More

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http://dx.doi.org/10.1021/acssensors.0c00752DOI Listing

Sequence-Specific Recognition of HIV-1 DNA with Solid-State CRISPR-Cas12a-Assisted Nanopores (SCAN).

ACS Sens 2020 May 8;5(5):1273-1280. Epub 2020 May 8.

Department of Electrical Engineering, Pennsylvania State University, University Park, Pennsylvania 16802, United States.

Nucleic acid detection methods are crucial for many fields such as pathogen detection and genotyping. Solid-state nanopore sensors represent a promising platform for nucleic acid detection due to its unique single molecule sensitivity and label-free electronic sensing. Here, we demonstrated the use of the glass nanopore for highly sensitive quantification of single-stranded circular DNAs (reporters), which could be degraded under the trans-cleavage activity of the target-specific CRISPR-Cas12a. Read More

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http://dx.doi.org/10.1021/acssensors.0c00497DOI Listing

Chip-Level Integration of Covalent Organic Frameworks for Trace Benzene Sensing.

ACS Sens 2020 May 13;5(5):1474-1481. Epub 2020 May 13.

Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, 117585 Singapore.

State-of-the-art chemical sensors based on covalent organic frameworks (COFs) are restricted to the transduction mechanism relying on luminescence quenching and/or enhancement. Herein, we present an alternative methodology via a combination of in situ-grown COF films with interdigitated electrodes utilized for capacitive benzene detection. The resultant COF-based sensors exhibit highly sensitive and selective detection at room temperature toward benzene vapor over carbon dioxide, methane, and propane. Read More

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http://dx.doi.org/10.1021/acssensors.0c00495DOI Listing

Reference Electrodes Based on Ionic Liquid-Doped Reference Membranes with Biocompatible Silicone Matrixes.

ACS Sens 2020 May 18. Epub 2020 May 18.

Department of Chemistry, University of Minnesota, 207 Pleasant St. SE, Minneapolis, Minnesota 55455, United States.

Many reference electrodes with an ionic liquid-doped reference membrane contain a plasticizer that can gradually leach out into the sample. However, because many common plasticizers are known to be endocrine disruptors and may induce inflammatory reactions, they are preferably avoided for wearable or implantable sensors. Therefore, this work tested polymeric reference electrode membranes prepared by solvent casting from seven commercially available biocompatible silicones that are widely used in implantable devices. Read More

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http://dx.doi.org/10.1021/acssensors.0c00363DOI Listing

Epidermal Enzymatic Biosensors for Sweat Vitamin C: Toward Personalized Nutrition.

ACS Sens 2020 May 18. Epub 2020 May 18.

Department of Nanoengineering, University of California, La Jolla, 92093 San Diego, California, United States.

Recent advances in wearable sensor technologies offer new opportunities for improving dietary adherence. However, despite their tremendous promise, the potential of wearable chemical sensors for guiding personalized nutrition solutions has not been reported. Herein, we present an epidermal biosensor aimed at following the dynamics of sweat vitamin C after the intake of vitamin C pills and fruit juices. Read More

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http://dx.doi.org/10.1021/acssensors.0c00604DOI Listing

3D-Printed Microfluidic Devices for Enhanced Online Sampling and Direct Optical Measurements.

ACS Sens 2020 May 14. Epub 2020 May 14.

The Department of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore, Maryland 21250, United States.

3D printing has emerged as a robust technique to fabricate reliable and reproducible microfluidic devices. However, a limitation of 3D-printed devices has been the low transparency even when printed in a "clear" material. There are currently no reports regarding direct optical measurements through a 3D-printed device. Read More

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http://dx.doi.org/10.1021/acssensors.0c00507DOI Listing

Ultrasensitive Photoelectrochemical Detection of MicroRNA on Paper by Combining a Cascade Nanozyme-Engineered Biocatalytic Precipitation Reaction and Target-Triggerable DNA Motor.

ACS Sens 2020 May 13;5(5):1482-1490. Epub 2020 May 13.

School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China.

Developing efficient strategies for sensitive detection of microRNAs, the noncoding bioactive molecules and well-established biomarkers, has aroused great interests due to its great potential values in genetic and pathological analyses. Herein, a highly selective and disposable paper-based photoelectrochemical (PEC) sensor was rationally designed for sensing microRNA based on simple self-assembly of a target-triggerable DNA motor and nanozyme-catalyzed multistage biocatalytic precipitation reaction. Specifically, a brand-new type II heterojunction of TiO-CeO nanotubes decorated with carbon fiber paper (CFP) was first prepared, which gave an enhanced photoreactive surface and realized fast electron transport and extraction, markedly accelerating photoelectric conversion efficiency of the sensor. Read More

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http://dx.doi.org/10.1021/acssensors.0c00632DOI Listing