Search Our Scientific Publications & Authors

Microb Drug Resist 2021 Jun 15. Epub 2021 Jun 15.

Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.

We modified rapid polymyxin Nordmann-Poirel (RPNP) test, called rapid colistin disk elution (RCDE) test, for detecting colistin resistance in Gram-negative bacilli and evaluated its performance compared with colistin broth disk elution (CBDE) test recommended by Clinical and Laboratory Standards Institute (CLSI). The RCDE test was performed by using a 10-μg colistin disk in 2.7 mL volume (final colistin concentration of 3. Read More

Int J Syst Evol Microbiol 2021 Jun;71(6)

Department of Biotechnology, Institute of Life Sciences, Nalco Square, Bhubaneswar - 751023, India.

A novel strain of a member of the genus strain PS-1, was isolated from the skin of fresh water pufferfish () collected from Mahanadi River, India. Cells were Gram-stain-negative, aerobic, coccoid and non-motile. The predominant polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE) and phospholipid (PL) and the cell wall sugars were glucose, galactose and ribose. Read More

BMC Microbiol 2021 Jun 14;21(1):179. Epub 2021 Jun 14.

Taihu Laboratory for Lake Ecosystem Research, State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, 210008, Nanjing, China.

Background: Bacterial community play a key role in environmental and ecological processes in river ecosystems. Rivers are used as receiving body for treated and untreated urban wastewaters that brings high loads of sewage and excrement bacteria. However, little is known about the bacterial community structure and functional files in the rivers around the eutrophic Chaohu Lake, the fifth largest freshwater lake in China, has been subjected to severe eutrophication and cyanobacterial blooms over the past few decades. Read More

Chem Sci 2019 Dec 10;11(5):1316-1334. Epub 2019 Dec 10.

Department of Chemical Biology, Helmholtz Centre for Infection Research Inhoffenstrasse 7 38124 Braunschweig Germany

There is an alarming scarcity of novel chemical matter with bioactivity against multidrug-resistant Gram-negative bacterial pathogens. Cystobactamids, recently discovered natural products from myxobacteria, are an exception to this trend. Their unusual chemical structure, composed of oligomeric -aminobenzoic acid moieties, is associated with a high antibiotic activity through the inhibition of gyrase. Read More

Environ Pollut 2021 Jun 2;286:117505. Epub 2021 Jun 2.

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, PR China.

The accumulation of estrogens in aquatic environments has drawn increasing public concern due to their adverse effects on aquatic ecosystems and human health. Bacteria play important roles in eliminating estrogens from the environment, but knowledge of the identity and functions of the microorganisms involved in metabolizing these steroid hormones in the natural microbial communities is lacking. Here, we added C-17β-estradiol (C-E2) to sediments collected from Zhushan (ZS) Bay, Meiliang (ML) Bay, Gonghu (GH) Bay, and the central area (CA) of the Taihu Lake. Read More

Sci Total Environ 2021 Jun 2;791:148228. Epub 2021 Jun 2.

College of Environmental Science and Engineering, Dalian Maritime University, Dalian, China.

It remains unclear whether excessive nitrogen additions lead to the degradation of Suaeda salsa (S. salsa) by affecting the nitrogen pool, enzyme activities, and bacterial community structure of wetland soils. This study investigated the effect of five added nitrogen concentrations (0, 1, 2, 4, and 6 mmol L N with NHNO = group C, group L, group M, group H, and group G, respectively) on nitrogen uptake by S. Read More

J Pak Med Assoc 2021 Apr;71(4):1144-1147

Department of Microbiology, Fauji Foundation Hospital, Rawalpindi, Pakistan.

Objective: To evaluate the phenotypic detection of extended-spectrum betalactamase in multidrug-resistant acinetobacter baumannii.

Methods: The cross-sectional study was conducted at the Department of Microbiology, Fauji Foundation Hospital, Rawalpindi, Pakistan, from August 2018 to April 2019, after the ethical approval from the Institutional Review Committee. Consecutive Non- probability sampling technique was used, and comprised clinical specimens, including pus, blood, sputum, urine, tracheal tubes and canula double lumen, which were processed using standard protocols. Read More

Antimicrob Agents Chemother 2021 Jun 14:AAC0264620. Epub 2021 Jun 14.

Anti-Infective Research Laboratory, Department of Pharmacy Practice, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, USA.

Cefiderocol (CFDC), a novel siderophore cephalosporin, demonstrates strong activity against multidrug-resistant (MDR) Acinetobacter baumannii. Limited studies have evaluated CFDC alone and in combination with other Gram-negative antibiotics against MDR A. baumannii isolates. Read More

Drug Deliv Transl Res 2021 Jun 14. Epub 2021 Jun 14.

Department of Emergency Medicine, Taipei Veterans General Hospital, 11217, Taipei, Taiwan.

There is an urgent need for treatments for hydrofluoric acid (HF) burns and their derivative problems that prevent hydrogen ion dissociation and fluoride ion binding to tissues. This study evaluated the ability of chitosan-based hydrogels combined with a buffer solution containing either boric acid or Tris and calcium gluconate (CHS-BA-CG and CHS-Tris-CG) to repair HF burn wounds and prevent wound infections. We assessed calcium release rates and biocompatability and constructed a mouse HF burn model to assess the tissue repair effects of the hydrogels. Read More

Appl Microbiol Biotechnol 2021 Jun 14. Epub 2021 Jun 14.

School of Public Health, Lanzhou University, Lanzhou, 730000, China.

Skin microbiomes function directly in human health and are affected by various external and internal factors. However, few studies have showed the variation of human skin microbiota at multiple body sites in individuals of different national origin living in the same environment. Here, using 16S rRNA sequencing, we investigated the diversity and function of skin microbiomes in different body locations of Chinese and Pakistani individuals from a single college in China. Read More

Bio Protoc 2021 May 5;11(9):e4000. Epub 2021 May 5.

Department of Chemistry, Indiana University, Bloomington, IN 47405 7102, USA.

Hydrogen sulfide (HS) is emerging as an important modulator in bacterial cytoprotection against the host immune response in infected animals, which may well be attributed to downstream highly oxidized sulfur species, termed reactive sulfur species (RSS), derived from HS. One mechanism by which HS/RSS may signal in the cell is through proteome -sulfuration (persulfidation), which is the conversion of protein thiols (-SH) to protein persulfides (-SSH). While several analytical methods have been developed to profile sites of protein persulfidation, few have been applied to bacterial cells. Read More

Bio Protoc 2021 Apr 20;11(8):e3997. Epub 2021 Apr 20.

School of Biological Sciences, Monash University, Clayton, VIC, Australia.

Lysogenic phages can integrate into their bacterial host's genome, potentially transferring any genetic information they possess including virulence or resistance genes, and are therefore routinely excluded from therapeutic applications. Lysogenic behavior is typically seen in phages that create turbid plaques or possess subpar bactericidal activity; yet, these are not definitive indicators. As a result, the presence of integrase genes is often used as a hallmark for lysogenic behavior; however, the accuracy of genetic screening for lysogeny depends on the quality of the extraction, sequencing and assembly of the phage genome, and database comparison. Read More

Biomed Res Int 2021 22;2021:9957112. Epub 2021 May 22.

Epidemiology and Public Health Unit, Cameroon Pasteur Centre, Member of Institut Pasteur International Network, Yaoundé, Cameroon.

Introduction: Antimicrobial resistance represents a growing public health threat. One of the World Health Organization's strategic objectives is "strengthening knowledge through surveillance and research." Sub-Saharan African countries are still far from achieving this objective. Read More

Front Vet Sci 2021 28;8:649372. Epub 2021 May 28.

Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University Ithaca, NY, United States.

The ruminant gut microbial community's importance has been widely acknowledged due to its positive roles in physiology, metabolism, and health maintenance. Diarrhea has been demonstrated to cause adverse effects on gastrointestinal health and intestinal microecosystem, but studies regarding diarrheal influence on gut microbiota in have been insufficient to date. Here, this study was performed to investigate and compare gut microbial composition and variability between healthy and diarrheic . Read More

Front Mol Biosci 2021 28;8:659390. Epub 2021 May 28.

Department of Laboratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

Sepsis is a common life-threatening disease in the intensive care unit (ICU) that is usually treated empirically without pathogen identification. As a non-invasive and high-throughput technology, plasma microbial cell-free DNA (mcfDNA) sequencing can detect unknown pathogens independent of previous clinical or laboratory information. In this study, a total of 199 cases suspected of bloodstream infection (BSI) from January 2020 to June 2020 were collected, and potential pathogens were detected by simultaneous blood culture and plasma mcfDNA sequencing. Read More

J Gen Appl Microbiol 2021 Jun 12. Epub 2021 Jun 12.

Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences.

Probiotics have been shown to improve microbial compositions in animal intestine and feces, but the effects of probiotic administration on airborne microbial composition in animal houses remain unclear. In this study, we investigated the effects of dietary Enterococcus faecalis on the bacterial community structure in the air of piglet and layer hen houses. Indoor air and feces from piglet and layer hen houses were sampled after supplementing E. Read More

Ann Clin Microbiol Antimicrob 2021 Jun 12;20(1):43. Epub 2021 Jun 12.

College of Medicine, Alfaisal University, Riyadh, Saudi Arabia.

Background: Studying time-related changes in susceptible pathogens causing healthcare-associated infections (HAIs) is vital in improving local antimicrobial and infection control practices.

Objectives: Describe susceptibility patterns to several antimicrobials in gram-positive and gram-negative pathogens isolated from patients causing HAIs at three private tertiary care hospitals in Saudi Arabia over a 5-year period.

Methods: Data on trends of antimicrobial susceptibility among bacteria causing HAIs events in children and adults at three tertiary private hospitals located in Riyadh and Qassim, Saudi Arabia, were collected retrospectively between 2015 and 2019 using the surveillance data datasets. Read More

J Hazard Mater 2021 May 27;418:126091. Epub 2021 May 27.

Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address:

Lignocellulosic biomass represents an unlimited and ubiquitous energy source, which can effectively address current global challenges, including climate change, greenhouse gas emissions, and increased energy demand. However, lignocellulose recalcitrance hinders microbial degradation, especially in case of contaminated materials such as creosote (CRO)-treated wood, which necessitates appropriate processing in order to eliminate pollution. This study might be the first to explore a novel bacterial consortium SST-4, for decomposing birchwood sawdust, capable of concurrently degrading lignocellulose and CRO compounds. Read More

J Glob Antimicrob Resist 2021 Jun 9. Epub 2021 Jun 9.

Cellular and Molecular Pharmacology, Louvain Drug Research Institute, Université catholique de Louvain, Brussels, Belgium.

Environ Res 2021 Jun 10;200:111455. Epub 2021 Jun 10.

Laboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological Engineering, Korea University, Seoul, 02841, Republic of Korea. Electronic address:

Cyanobacterial blooms caused mainly by Microcystis aeruginosa could be controlled using chemical and biological agents such as HO, antagonistic bacteria, and enzymes. Little is known about the possible toxic effects of bacterial membrane pigments on M. aeruginosa cells. Read More

FEBS Lett 2021 Jun 12. Epub 2021 Jun 12.

College of Pharmacy, Chung-Ang University, Seoul, 06974, Republic of Korea.

Thioredoxin reductase (TrxR) is a central component in the thioredoxin system involved in catalyzing the reduction of thioredoxin, which is critical for organism survival. Because this system is essential, it is a promising target for novel antimicrobial agents. Herein, we solved the 1. Read More

FEMS Microbiol Lett 2021 Jun;368(10)

Water Sci Technol 2021 Jun;83(11):2640-2651

Tianjin IKWEN Water Treatment Co., Ltd, Tianjin 300000, China.

A continuous-flow Anaerobic/Anoxic/Oxic (A/O) system was operated at different organic concentrations to systematically investigate the effect on the nutrient removal, secretion characteristics of extracellular polymer, phosphorus forms transformation and changes in functional flora in this system. The results showed that high organic loading was more conducive to promote the secretion of extracellular polymeric substance (EPS), the increase of polysaccharide content was more obvious compared with protein, the impact of organic loading on the components of loosely bound EPS (LB-EPS) was higher than that of tight-bound EPS (TB-EPS). Phosphorus in sludge floc mainly existed in the form of inorganic phosphorus (IP), and IP mainly existed in the form of apatite inorganic phosphorus (AP). Read More

iScience 2021 May 26;24(5):102476. Epub 2021 Apr 26.

Talent Highland and Center for Gut Microbiome Research of Med-X Institute, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China.

Bacterial two-component regulatory systems are ubiquitous environment-sensing signal transducers involved in pathogenesis and antibiotic resistance. The two-component regulatory system AdeRS is made up of a sensor histidine kinase AdeS and a cognate response regulator AdeR, which together reduce repression of the multidrug-resistant efflux pump AdeABC. Herein we demonstrate that an N-terminal intrinsically disordered tail in AdeR is important for the upregulation of expression, although it greatly increases the susceptibility of AdeR to proteasome-mediated degradation. Read More

Front Microbiol 2021 25;12:663210. Epub 2021 May 25.

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

is the primary cause of community-acquired foodborne infections, so its resistance to antimicrobials, such as aminoglycosides, is a public health issue. Of concern, aminoglycoside resistance in is increasing rapidly. Here, we performed a retrospective study evaluating the prevalence of harboring -mediated aminoglycoside resistance in community-acquired infections and in food or environmental sources. Read More

Sci Rep 2021 Jun 10;11(1):12248. Epub 2021 Jun 10.

Botucatu Medical School, Sao Paulo State University Julio de Mesquita Filho (UNESP), Botucatu, Brazil.

Peritonitis due to gram-negative bacilli (GNB), particularly nonfermenting GNB (NF-GNB), is a serious complication of peritoneal dialysis with a low resolution rate. Beyond the patient's condition, microbiological properties such as antimicrobial resistance, biofilm production and other virulence factors can explain the poor outcomes. This study aimed to evaluate the influence of patient condition, microbiological characteristics, including biofilm production, and treatment on peritonitis outcome. Read More

Food Res Int 2021 Jul 19;145:110417. Epub 2021 May 19.

Guangxi Crop Genetic Improvement and Biotechnology Key Lab, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China. Electronic address:

Microorganisms in grape skins play vital roles in grapevine health, productivity, wine quality and organoleptic properties. To investigate microbial diversity of muscadine grape skins, 16S and ITS sequences of 30 samples from six muscadine (Muscadinia rotundifolia Michx.) cultivars grown in Guangxi, China, were sequenced using Illumina Novaseq platform. Read More

Food Res Int 2021 Jul 11;145:110409. Epub 2021 May 11.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, China; Synergetic Innovation Center of Food Safety and Quality Control of Jiangsu Province, Wuxi 214122, China.

Presently, because of the extraordinary roles and potential applications, rare sugars turn into a focus point for countless researchers in the field of carbohydrates. l-ribose and l-ribulose are rare sugars and isomers of each other. This aldo and ketopentose are expensive but can be utilized as an antecedent for the manufacturing of various rare sugars and l-nucleoside analogue. Read More

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2021 May;33(5):587-592

Department of Intensive Care Unit, Taian City Central Hospital, Taian 271000, Shandong, China.

Objective: To identify the distribution of research hotspots and frontiers of multidrug-resistant bacteria in intensive care units in China through the method of visualization, and to predict future research directions, analyze the research development process, so as to provide reference basis for further research in this field.

Methods: Studies related to multidrug-resistant bacteria in intensive care units published in China from 2000 to 2019 by CNKI were reviewed. According to the keywords by CiteSpace 5. Read More

Clin Microbiol Infect 2021 Jun 7. Epub 2021 Jun 7.

Department of Pharmacy and Biomedical Sciences, School of Molecular Sciences, College of Science, Health and Engineering, La Trobe University, Victoria, Australia. Electronic address:

Background: A wide range of bacterial infections occur in COVID-19 patients, particularly, in those with severe coronaviral disease. Some of these are community acquired co-infections.

Objective: To review recent data which indicate the occurrence of hospital-onset bacterial infections, including with antibiotic-resistant isolates, in COVID-19 patients. Read More