4,616 results match your criteria Acanthamoeba


Dual 0.02% chlorhexidine digluconate - 0.1% disodium EDTA loaded thermosensitive ocular gel for Acanthamoeba keratitis treatment.

Int J Pharm 2018 Dec 12. Epub 2018 Dec 12.

Service Pharmaceutique, Plateforme Fripharm, Groupe Hospitalier Centre Edouard Herriot, Hospices Civils de Lyon, 5, Place d'Arsonval, F-69437 Lyon cedex 03, France; Université de Lyon, Laboratoire de Pharmacie Galénique Industrielle, UMR-CNRS 5305, Plateforme Fripharm, ISPB-Faculté de Pharmacie, Université Claude Bernard Lyon 1, 8, avenue Rockefeller, F-69373 Lyon Cedex 08, France. Electronic address: http://fripharm.com.

Poor bioavailability and low residence time limit the efficiency of conventional biguanide-based eye drops against Acanthamoeba keratitis. The aim of this work was to formulate an original anti-amoebic thermoreversible ocular gel combining biguanide and metalloproteases inhibitor - chelating agent. Chlorhexidine digluconate (CHX)-ethylenediaminetetraacetic acid disodium salt (NaEDTA) were compounded in poloxamer 407 saline solution. Read More

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December 2018

New isolates of pandoraviruses: contribution to the study of replication cycle steps.

J Virol 2018 Dec 12. Epub 2018 Dec 12.

Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

Giant viruses are complex members of the virosphere, exhibiting outstanding structural and genomic features. Among these viruses, the pandoraviruses are one of the most intriguing members, exhibiting giant particles and genomes presenting up to 2.5 Mb, with many genes having no known function. Read More

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December 2018

Removal Efficacy of Opportunistic Pathogens and Bacterial Community Dynamics in Two Drinking Water Treatment Trains.

Small 2018 Dec 7:e1804436. Epub 2018 Dec 7.

Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University, Shanghai, 200433, China.

Drinking water treatment processes (DWTPs) impact pathogen colonization and microbial communities in finished water; however, their efficacies against opportunistic pathogens are not fully understood. In this study, the effects of treatment steps on the removal of Legionella spp., Legionella pneumophila, nontuberculous mycobacteria, Mycobacterium avium, and two amoeba hosts (Vermamoeba vermiformis, Acanthamoeba) are evaluated in two parallel trains of DWTPs equipped with different pretreatment units. Read More

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December 2018
1 Read

Exotic Tourist Destinations and Transmission of Infections by Swimming Pools and Hot Springs-A Literature Review.

Int J Environ Res Public Health 2018 Dec 3;15(12). Epub 2018 Dec 3.

Department of Biomedical Sciences, University of West Attica, 12243 Egaleo, Greece.

A growing number of people undertake international travel, and yet faster growth of such travel is expected in the tropics. Information on the hazards presented by pool and hot spring waters in tropical countries is very limited. This review aims to collate available information on pool water quality, alongside data on cases and outbreaks associated with swimming in pools in tropical regions affecting both local populations and travellers. Read More

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December 2018
1 Read

Granulomatous amebic encephalitis caused by Acanthamoeba in an immuncompetent child.

Turk J Pediatr 2018 ;60(3):340-343

Division of Infectious Diseases, İstanbul University İstanbul Faculty of Medicine, İstanbul, Turkey.

Sütçü M, Aktürk H, Gülümser-Şişko S, Acar M, Erol OB, Somer A, Bilgiç B, Salman N. Granulomatous amebic encephalitis caused by Acanthamoeba in an immuncompetent child. Turk J Pediatr 2018; 60: 340-343. Read More

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January 2018
1 Read

Molecular and biochemical characterization of key enzymes in the cysteine and serine metabolic pathways of Acanthamoeba castellanii.

Parasit Vectors 2018 Nov 26;11(1):604. Epub 2018 Nov 26.

Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.

Background: Acanthamoeba spp. can cause serious human infections, including Acanthamoeba keratitis, granulomatous amoebic encephalitis and cutaneous acanthamoebiasis. Cysteine biosynthesis and the L-serine metabolic pathway play important roles in the energy metabolism of Acanthamoeba spp. Read More

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November 2018
1 Read

Acanthamoeba keratitis in Mexico: Report of a clinical case and importance of sensitivity assays for a better outcome.

Exp Parasitol 2018 Nov 22;196:22-27. Epub 2018 Nov 22.

University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Tenerife, Canary Island, Spain.

Acanthamoeba keratitis (AK) is a sight-threatening corneal infection. The early symptoms include redness, pain, photophobia and intense tearing. Chronic infection usually progresses to stromal inflammation, ring ulcers, corneal opacification and hypopyon. Read More

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November 2018
1 Read

Genomic Signatures Among Acanthamoeba polyphaga Entoorganisms Unveil Evidence of Coevolution.

J Mol Evol 2018 Nov 20. Epub 2018 Nov 20.

Laboratório de Genética Evolutiva Paulo Leminsk, Departamento de Biologia Molecular, Centro de Ciencias Exatas e da Natureza, Universidade Federal da Paraíba, João Pessoa, Brazil.

The definition of a genomic signature (GS) is "the total net response to selective pressure". Recent isolation and sequencing of naturally occurring organisms, hereby named entoorganisms, within Acanthamoeba polyphaga, raised the hypothesis of a common genomic signature despite their diverse and unrelated evolutionary origin. Widely accepted and implemented tests for GS detection are oligonucleotide relative frequencies (OnRF) and relative codon usage (RCU) surveys. Read More

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November 2018
1 Read

Discovery of myosin I and Pollard-san.

Authors:
Fumio Oosawa

Biophys Rev 2018 Nov 16. Epub 2018 Nov 16.

Department of Physics, Graduate School of Science, Nagoya University, Nagoya, 464-8602, Japan.

In this short review, I describe a brief history of the discovery of myosin I isolated from Acanthamoeba in 1973 by Tom Pollard and Ed Korn. Today, myosins form a large "family tree" that includes more than 30 types of myosins. I discuss the importance of the relationship among actin, myosin, and other actin-binding proteins, many of which were pioneered by Pollard-san ("-san" is a Japanese honorific suffix showing respect, politeness and friendship). Read More

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November 2018

Anti- Activity of Brominated Sesquiterpenes from .

Mar Drugs 2018 Nov 11;16(11). Epub 2018 Nov 11.

Instituto Universitario de Bio-Orgánica Antonio González (IUBO AG), Centro de Investigaciones Biomédicas de Canarias (CIBICAN), Universidad de La Laguna (ULL), Avda. Astrofísico F. Sánchez, 2, 38206 La Laguna, Tenerife, Spain.

Focused on our interest to develop novel antiparasistic agents, the present study was aimed to evaluate the biological activity of an extract of collected in Baja California Sur, Mexico, against an Neff strain. Bioassay-guided fractionation allowed us to identify the amoebicidal diastereoisomers α-bromocuparane () and α-isobromocuparane (). Furthermore, bromination of the inactive laurinterol () and isolaurinterol () yielded four halogenated derivatives, ()⁻(), which improved the activity of the natural sesquiterpenes. Read More

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November 2018
2.853 Impact Factor

Effect of 2, 6-Dichlorobenzonitrile on Amoebicidal Activity of Multipurpose Contact Lens Disinfecting Solutions.

Korean J Parasitol 2018 Oct 31;56(5):491-494. Epub 2018 Oct 31.

Department of Parasitology, Dong-A University College of Medicine, Busan 49201, Korea.

Multipurpose contact lens disinfecting solutions (MPDS) are widely used to cleanse and disinfect microorganisms. However, disinfection efficacy of these MPDS against Acanthamoeba cyst remain insufficient. 2, 6-dichlorobenzonitrile (DCB), a cellulose synthesis inhibitor, is capable of increasing the amoebical effect against Acanthamoeba by inhibiting its encystation. Read More

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October 2018
4 Reads

Molecular and Biochemical Properties of a Cysteine Protease of Acanthamoeba castellanii.

Korean J Parasitol 2018 Oct 31;56(5):409-418. Epub 2018 Oct 31.

Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju 52727, Korea.

Acanthamoeba spp. are free-living protozoa that are opportunistic pathogens for humans. Cysteine proteases of Acanthamoeba have been partially characterized, but their biochemical and functional properties are not clearly understood yet. Read More

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October 2018
3 Reads

Unusual 18S rDNA of Acanthamoeba containing intron turned out to be a T5/T4 chimera.

Parasitol Res 2018 Nov 12. Epub 2018 Nov 12.

CHLAREAS, 12, rue du Maconnais, F-54500, Vandœuvre-lès-Nancy, France.

The free-living amoebae of the genus Acanthamoeba are widely investigated for their diversity and evolution. Studies usually employ biomolecular methods targeting the 18S rRNA gene, assigning strains according to a well-established genotyping system. Strains from at least four genotypes contain introns in their rDNA. Read More

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November 2018
4 Reads

Gold nanoparticles conjugation enhances antiacanthamoebic properties of Nystatin, Fluconazole and Amphotericin B.

J Microbiol Biotechnol 2018 Nov 2. Epub 2018 Nov 2.

Department of Biological Sciences, School of Science and Technology, Sunway University, Subang Jaya 47500, Selangor, Malaysia.

Parasitic infections have remained a significant burden on human and animal health. In part, this is due to lack of clinically-approved novel antimicrobials and a lack of interest by the pharmaceutical industry. An alternative approach is to modify existing clinically-approved drugs for efficient drugs delivery formulations to ensure minimum inhibitory concentration is achieved at the target site. Read More

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November 2018
3 Reads

A Mutation in the Gene Alters the Physicochemical Properties of the Bacterial Cell Wall and Reduces Survival inside .

Int J Mol Sci 2018 Nov 8;19(11). Epub 2018 Nov 8.

Department of Comparative Anatomy and Anthropology, Institute of Biology, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, Akademicka 19 St., 20-033 Lublin, Poland.

In our previous report, we had shown that the free-living amoeba influenced the abundance, competiveness, and virulence of NZP2213, the microsymbiont of agriculturally important plants of the genus . The molecular basis of this phenomenon; however, had not been explored. In the present study, we demonstrated that , the -acetyltransferase encoding gene located in the lipopolysaccharide (LPS) synthesis cluster of , was responsible for maintaining the protective capacity of the bacterial cell envelope, necessary for the bacteria to fight environmental stress and survive inside amoeba cells. Read More

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November 2018
5 Reads

[Contact lens complications : Diagnosis and treatment].

Ophthalmologe 2018 Dec;115(12):1087-1102

Praxis und Expert-Institut, Gammertinger Str. 33, 88499, Riedlingen, Deutschland.

Contact lens complications are all pathological alterations of the eyes caused by wearing contact lenses. Clinical manifestations particularly affect the cornea and conjunctiva. A differentiation is made between undesired manifestations and disease conditions that at least temporarily make the further wearing of contact lenses impossible. Read More

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December 2018
3 Reads

Potentially pathogenic genera of free-living amoebae coexisting in a thermal spring.

Exp Parasitol 2018 Dec 26;195:54-58. Epub 2018 Oct 26.

Departamento de Ciencias Agronómicas y Veterinarias, Mexico; Programa de Doctorado en Ciencias en Biotecnología, Mexico. Electronic address:

Little is known about the prevalence of Balamuthia mandrillaris within the environment due to its difficult isolation, but once an axenic culture is established, it is relatively easy to maintain. As most of the time researchers are interested mainly in isolating B. mandrillaris from environmental samples, the flora that accompanies it becomes second in importance. Read More

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December 2018
1 Read

Box Behnken design of siRNA-loaded liposomes for the treatment of a murine model of ocular keratitis caused by Acanthamoeba.

Colloids Surf B Biointerfaces 2019 Jan 18;173:725-732. Epub 2018 Oct 18.

Graduate Program in Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, Brazil. Electronic address:

Acanthamoeba keratitis is an ophthalmic disease with no specific treatment that specially affects contact lens users. The silencing of serine phosphatase (SP) and glycogen phosphorylase (GP) proteins produced by Acanthamoeba has been shown to significantly reduce the cytopathic effect, although no vehicle was proposed yet to deliver the siRNA sequences to the trophozoites. In this study, PEGylated cationic liposomes were proposed and optimized using Box-Behnken design. Read More

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January 2019
3 Reads

Molecular characterization and phylogenetic analysis of Acanthamoeba isolates in tap water of Beni-Suef, Egypt.

Acta Parasitol 2018 Dec;63(4):826-834

Department of Medical Parasitology, College of Medicine, Beni-Suef University, Beni-Suef, Egypt.

The genus Acanthamoeba is a free-living amoeba widely distributed in various aquatic environments. It is an etiologic cause of amoebic encephalitis and keratitis particularly for immunocompromised individuals. The purpose of the present study was to investigate Acanthamoeba species prevalence in household and hospital potable water in Beni-Suef governorate, Egypt, and to employ sequencing methods to identify positive Acanthamoeba species isolates and their potential health risks. Read More

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December 2018
6 Reads
0.960 Impact Factor

Evidence of human-like Ca Channels and Effects of Ca Channel blockers in Acanthamoeba castellanii.

Chem Biol Drug Des 2018 Oct 25. Epub 2018 Oct 25.

The Aga Khan University, Karachi, Pakistan.

We for the first time report the primitive origins of eukaryotic voltage-gated calcium channels [Cav] in the eukaryotic time-line. The evolution of Cav in eukaryotes is an area of interest for biologists worldwide. The CLAN CL0030 and its family Ion_Trans 2 PF 07885 has been known to be present in prokaryotes, but the origin of these channels in Acanthamoeba are yet to be determined. Read More

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October 2018
5 Reads
2.485 Impact Factor

Comparative in vitro effectiveness of a novel contact lens multipurpose solution on Acanthamoeba castellanii.

J Ophthalmic Inflamm Infect 2018 Oct 24;8(1):19. Epub 2018 Oct 24.

Department of Microbiology and Immunology, Tulane School of Medicine, New Orleans, LA, 70102, USA.

Background: A multipurpose contact lens cleaning solution (MPS) containing novel active ingredients under development was compared to two commercially available MPS solutions for effectiveness against Acanthamoeba isolates.

Methods: The Acanthamoeba isolate A. castellanii was propagated for trophozoite or cyst-containing cultures for the purpose of assessment of effectiveness of each MPS. Read More

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October 2018
1 Read

Identification and ultrastructural characterization of Acanthamoeba bacterial endocytobionts belonging to the Alphaproteobacteria class.

PLoS One 2018 24;13(10):e0204732. Epub 2018 Oct 24.

Medical Sciences, School of Medicine, International Medical University, Kuala Lumpur, Malaysia.

The detection and identification of two endocytobiotic bacterial strains, one affiliated to the "Candidatus Caedibacter acanthamoebae"/"Ca. Paracaedimonas acanthamoeba", and another to the endosymbiont of Acanthamoeba UWC8 and "Ca. Jidaibacter acanthamoeba" are described. Read More

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October 2018
3 Reads

Drug targeting in Acanthamoeba keratitis: rational of using drugs that are already approved for ocular use in non-keratitis indications.

Eye (Lond) 2018 Oct 24. Epub 2018 Oct 24.

Department of Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan.

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October 2018
1.900 Impact Factor

Corneal "Plaque" formation after anti-acanthamoeba therapy in acanthamoeba keratitis.

Indian J Ophthalmol 2018 Nov;66(11):1623-1624

Department of Microbiology, Dr. Sapra's Lab, Gurugram, Haryana, India.

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November 2018
1 Read

Applications of Invertebrate Animal Models to Dimorphic Fungal Infections.

J Fungi (Basel) 2018 10 19;4(4). Epub 2018 Oct 19.

School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, São Paulo 14800-903, Brazil.

Dimorphic fungi can be found in the yeast form during infection and as hyphae in the environment and are responsible for a large number of infections worldwide. Invertebrate animals have been shown to be convenient models in the study of fungal infections. These models have the advantages of being low cost, have no ethical issues, and an ease of experimentation, time-efficiency, and the possibility of using a large number of animals per experiment compared to mammalian models. Read More

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October 2018
5 Reads

Clinically Approved Drugs against CNS Diseases as Potential Therapeutic Agents To Target Brain-Eating Amoebae.

ACS Chem Neurosci 2018 Oct 29. Epub 2018 Oct 29.

Department of Biological Sciences, School of Science and Technology , Sunway University , Selangor 47500 , Malaysia.

Central nervous system (CNS) infections caused by free-living amoebae such as Acanthamoeba species and Naegleria fowleri are rare but fatal. A major challenge in the treatment against the infections caused by these amoebae is the discovery of novel compounds that can effectively cross the blood-brain barrier to penetrate the CNS. It is logical to test clinically approved drugs against CNS diseases for their potential antiamoebic effects since they are known for effective blood-brain barrier penetration and affect eukaryotic cell targets. Read More

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October 2018
1 Read

Isolation and identification of Acanthamoeba from pond water of parks in a tropical and subtropical region in the Middle East, and its relation with physicochemical parameters.

BMC Microbiol 2018 Oct 19;18(1):139. Epub 2018 Oct 19.

Department of Medical Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.

Background: Free-living amoeba (FLA) are wide-spread protozoa that are found in different environmental sources including water, soil, dust, hospital units and ventilation areas. These amoebas can act as opportunistic or non-opportunistic pathogens. Among FLAs, some genera such as Acanthamoeba are important because of their potential pathogenic ability in humans. Read More

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October 2018
8 Reads
2.730 Impact Factor

Identification of free-living amoebae isolated from tap water in Istanbul, Turkey.

Exp Parasitol 2018 Dec 15;195:34-37. Epub 2018 Oct 15.

Molecular Parasitology, Institute of Specific Prophylaxis and Tropical Medicine, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Kinderspitalgasse 15, 1090, Vienna, Austria.

Free-living amoebae (FLA) are widely spread in the environment and also known to cause rare but often serious infections. The present work focuses on a local survey on FLA. It is essential to know the prevalence and distribution of these microorganisms in order to get infections caused by them under control. Read More

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December 2018
6 Reads

PPIases Mip and PpiB of contribute to surface translocation, growth at suboptimal temperature and infection.

Infect Immun 2018 Oct 15. Epub 2018 Oct 15.

Institut für Mikrobiologie, Technische Universität Braunschweig, 38106, Braunschweig

The gamma-proteobacterium is the causative agent of Legionnaires' disease, an atypical pneumonia that manifests itself with severe lung damage. , a common inhabitant of fresh water environments, replicates in free-living amoebae, and persists in biofilms in natural and man-made water systems. Its environmental versatility is reflected in its ability to survive and grow within a broad temperature range as well as its capability to colonize and infect a wide range of hosts including protozoa and humans. Read More

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October 2018
1 Read

Comparison of chlorhexidine disinfectant in vitro effect on environmental and ocular Acanthamoeba strains, the amoebic agents of human keratitis − an emerging sight-threatening corneal disease in Poland

Ann Parasitol 2018;64(3):229-233

Department of Medical Biology, Medical University of Warsaw, ul. Nowogrodzka 73, 02-018 Warsaw, Poland

Small amoebae belonging to the Acanthamoeba genus complete their life cycles in different environmental niches as free-living protists however some of them are facultative parasites that can cause severe disease in humans. The sight-threatening Acanthamoeba keratitis develops in immune-competent persons, mainly in contact lens wearers; it is detected with increasing frequency along with the spread of contact lens use. The high abundance of the amoebae in the environment is important for dispersion and transmission of the infections among humans. Read More

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January 2018
8 Reads

The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome.

Rev Soc Bras Med Trop 2018 Sep-Oct;51(5):716-719

Laboratório de Estudos da Relação Parasito-Hospedeiro, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO, Brasil.

We report an Acanthamoeba keratitis case associated with the use of contact lens in a 28-year-old female from Brasília, Brazil. Samples from corneal scraping and contact lens case were used for culture establishment, PCR amplification, and partial sequencing (fragments of ~400kb) of small subunit rDNA; both culture and PCR were positive. The sequence analyses of the cornea and of isolates from the contact lens case showed similarity with the T4 genotype. Read More

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October 2018
3 Reads

Fatal Granulomatous Amebic Encephalitis in a Heart Transplant Patient: Clinical, Radiographic, and Autopsy Findings.

J Neuropathol Exp Neurol 2018 Nov;77(11):1001-1004

Department of Pathology, Duke University Medical Center, Durham, North Carolina.

Granulomatous amebic encephalitis is a rare necrotizing infection of the CNS that occurs most commonly in immunocompromised individuals and is usually fatal. It is difficult to diagnose as the clinical symptoms and radiographic findings are often mistaken for other bacterial, viral, fungal, or protozoan infections. Herein, we present the case of a 69-year-old heart transplant recipient who suffered fulminant neurological decline ∼5 months after transplant. Read More

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November 2018
8 Reads

Non-granulomatous cerebellar infection by Acanthamoeba spp. in an immunocompetent host.

Infection 2018 Dec 4;46(6):885-889. Epub 2018 Oct 4.

Hospital Department of Specialized and Internal Medicine, Infectious Diseases Unit, University Hospital of Siena, Siena, Italy.

Acanthamoeba spp. is a free-living amoeba, frequently involved in keratitis by contact lens in immunocompetent hosts. Anecdotal reports associate Acanthamoeba spp. Read More

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December 2018
22 Reads

Isolation and molecular identification of Acanthamoeba spp. from hot springs in Mazandaran province, northern Iran.

J Water Health 2018 Oct;16(5):807-813

Toxoplasmosis Research Center, Mazandaran University of Medical Science, Sari, Iran E-mail: Student of Research Committee, Mazandaran University of Medical Science, Sari, Iran.

Acanthamoeba is a free-living protozoan that can be found in natural and artificial environments such as hot tubs, surface water and springs and can cause severe diseases including amoebic keratitis and granulomatous amoebic encephalitis. The present study was conducted owing to the lack of research regarding genotypes of Acanthamoeba in hot springs of Mazandaran province in northern Iran. Twenty-four water samples were collected from all hot springs in Mazandaran province. Read More

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October 2018
7 Reads

Ocular Parasitosis Caused by Protozoan Infection during Travel: Focus on Prevention and Treatment.

Int J Prev Med 2018 17;9:79. Epub 2018 Sep 17.

Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

International travel is rising quickly worldwide. Many people travel to tropical and subtropical areas, where there has been increasing exposure of travelers to infectious pathogens. Ocular parasitic infections are more prevalent in these geographical areas and they can lead to morbidity and mortality, often due to late or misdiagnosis due to the unfamiliarity of health staff with these diseases. Read More

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September 2018
8 Reads

Intracorneal injection of a detachable hybrid microneedle for sustained drug delivery.

Acta Biomater 2018 Oct 26;80:48-57. Epub 2018 Sep 26.

Department of Mechanical Engineering, Yonsei University, Seoul 03722, Republic of Korea. Electronic address:

There are increasing demands for long-term and controlled corneal drug delivery to treat various ocular diseases. Although biodegradable ocular inserts or contact lenses have been developed, the invasiveness and inefficiency of the approaches still need to be improved. Microneedle (MN) technology can deliver therapeutic molecules to the eye in a minimally invasive manner. Read More

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October 2018
17 Reads

Mimivirus-Encoded Nucleotide Translocator VMC1 Targets the Mitochondrial Inner Membrane.

J Mol Biol 2018 Dec 25;430(24):5233-5245. Epub 2018 Sep 25.

Institute of Biochemistry and Pathobiochemistry, Ruhr-University Bochum, 44780 Bochum, Germany. Electronic address:

Mimivirus (Acanthamoeba polyphaga mimivirus) was the first giant DNA virus identified in an amoeba species. Its genome contains at least 979 genes. One of these, L276, encodes a nucleotide translocator with similarities to mitochondrial metabolite carriers, provisionally named viral mitochondrial carrier 1 (VMC1). Read More

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December 2018
1 Read

Dynamics and determinants of amoeba community, occurrence and abundance in subtropical reservoirs and rivers.

Water Res 2018 Dec 5;146:177-186. Epub 2018 Sep 5.

Aquatic EcoHealth Group, Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China. Electronic address:

Free-living amoebae are widespread in freshwater ecosystems. Although many studies have investigated changes in their communities across space, the temporal variability and the drivers of community changes across different habitat types are poorly understood. A total of 108 surface water samples were collected on a seasonal basis from four reservoirs and two rivers in Xiamen city, subtropical China. Read More

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December 2018
6 Reads

In Vitro Amoebicidal Activity of Titanium Dioxide/UV-A Combination Against Acanthamoeba.

Invest Ophthalmol Vis Sci 2018 Sep;59(11):4567-4571

Service d'Ophtalmologie, Hôpitaux Universitaires de Strasbourg, Nouvel Hôpital Civil, Strasbourg, France.

Purpose: To assess the amoebicidal effect of titanium dioxide (TiO2)/UV-A combination against Acanthamoeba sp trophozoites and cysts.

Methods: The amoebicidal effect of the TiO2/UV-A combination was tested on trophozoites and cysts of clinical isolates of Acanthamoeba hatchetti and Acanthamoeba sp genotype T4, obtained from two severe cases of ulcerative keratitis. Samples of cultured Acanthamoeba were transferred to a 96-well plate. Read More

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September 2018
7 Reads

A Phylogenomic Study of Draft Genome Sequences Suggests Genetic Exchanges With Giant Viruses.

Front Microbiol 2018 6;9:2098. Epub 2018 Sep 6.

Institut de Recherche pour le Développement, Assistance Publique - Hôpitaux de Marseille, Microbes, Evolution, Phylogeny and Infection, and Institut Hospitalo-Universitaire - Méditerranée Infection, Aix-Marseille Université, Marseille, France.

are ubiquitous phagocytes predominant in soil and water which can ingest many microbes. Giant viruses of amoebae are listed among the -resisting microorganisms. Their sympatric lifestyle within amoebae is suspected to promote lateral nucleotide sequence transfers. Read More

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September 2018

Changes in the immune system in experimental acanthamoebiasis in immunocompetent and immunosuppressed hosts.

Parasit Vectors 2018 Sep 20;11(1):517. Epub 2018 Sep 20.

Department of Biology and Medical Parasitology, Pomeranian Medical University in Szczecin, 70-204, Szczecin, Poland.

Background: Acanthamoebiasis is most often found in patients with immune deficiency, with infections facilitated by the intake of immunosuppressive drugs. The host immune response to Acanthamoeba spp. infection is poorly understood. Read More

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September 2018
1 Read

Association Between Season, Temperature and Causative Organism in Microbial Keratitis in the UK.

Cornea 2018 Dec;37(12):1555-1560

Cornea Department, Manchester Royal Eye Hospital, Manchester, United Kingdom.

Purpose: Microbial keratitis (MK) is a major cause of corneal blindness worldwide. Variations in season and temperature can affect MK incidence due to specific causative organisms; however, few studies have examined these factors in the UK.

Methods: Retrospective review of all corneal scrapes from patients with MK presenting to Manchester Royal Eye Hospital, UK, between January 2004 and December 2015. Read More

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December 2018
1 Read

Herpes simplex virus keratitis mimicking Acanthamoeba keratitis: a clinicopathological correlation.

BMJ Case Rep 2018 Sep 19;2018. Epub 2018 Sep 19.

Department of Ophthalmology, New York Eye and Ear Infirmary of Mount Sinai, New York City, New York, USA.

A 36-year-old male, soft contact lens wearer was referred by his primary ophthalmologist for corneal ulcer of the right eye (OD), which was persistent despite topical fluoroquinolone therapy for 1 month. A ring-shaped infiltrate typically seen in Acanthamoeba infection was noted, and topical therapy with chlorhexidine and polyhexamethylene biguanide was initiated. However, the patient's condition deteriorated over the next several weeks; thus, diagnostic and therapeutic penetrating keratoplasty was performed. Read More

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September 2018
3 Reads

keratitis: confirmation of the UK outbreak and a prospective case-control study identifying contributing risk factors.

Br J Ophthalmol 2018 Dec 19;102(12):1621-1628. Epub 2018 Sep 19.

Corneal and External Disease Service, Moorfields Eye Hospital NHS Foundation Trust, London, UK

Background/aims: keratitis (AK) is a chronic debilitating corneal infection principally affecting contact lens (CL) users. Studies were designed to test claims that the UK incidence may have increased in 2012-2014 and to evaluate potential causes.

Methods: Annualised incidence data were collected from January 1984 to December 2016. Read More

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December 2018
1 Read

: An Expanding Family of Highly Diverse Large dsDNA Viruses Infecting a Wide Phylogenetic Range of Aquatic Eukaryotes.

Viruses 2018 09 18;10(9). Epub 2018 Sep 18.

Aix Marseille Univ, CNRS, IGS, Structural and Genomic Information Laboratory (UMR7256), Mediterranean Institute of Microbiology (FR3479), 163 Avenue de Luminy, F-13288 Marseille, France.

Since 1998, when Jim van Etten's team initiated its characterization, Chlorella virus 1 (PBCV-1) had been the largest known DNA virus, both in terms of particle size and genome complexity. In 2003, the -infecting Mimivirus unexpectedly superseded PBCV-1, opening the era of giant viruses, i.e. Read More

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September 2018

A Review of the Cytokine IL-17 in Ocular Surface and Corneal Disease.

Curr Eye Res 2018 Sep 19:1-10. Epub 2018 Sep 19.

d Institute of Ophthalmology , University College London , London , England.

Aim: To investigate the role of interleukin-17 in ocular surface and corneal disease. Ocular surface and corneal disease is a leading cause of blindness and is an ongoing challenge for the public health sector to implement effective therapies. The majority of cells in corneal lesions are derived primarily from neutrophils that induce inflammatory events that lead to tissue damage. Read More

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September 2018
2 Reads

Water related ocular diseases.

Authors:
Syed Shoeb Ahmad

Saudi J Ophthalmol 2018 Jul-Sep;32(3):227-233. Epub 2017 Nov 4.

Department of Ophthalmology, Queen Elizabeth Hospital, Kota Kinabalu, Malaysia.

A number of ocular diseases can be attributed to contaminated water and we have coined a term "Water-related ocular diseases (WRODs)" to denote this wide-spectrum of conditions. WRODs are directly related to human contact with water and can occur through toxic, allergic, inflammatory or infective mechanisms. The non-infective causes can include chemicals used to clean swimming pools, oil spills and water-sport related injuries. Read More

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November 2017
3 Reads

Comparative proteomic analysis of soluble and surface-enriched proteins from Acanthamoeba castellanii trophozoites.

Mol Biochem Parasitol 2018 Oct 8;225:47-53. Epub 2018 Sep 8.

Programa de Pós-Graduação em Microbiologia Agrícola e do Ambiente, Instituto de Ciências Básicas e da Saúde, UFRGS, Porto Alegre, RS, Brazil; Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, UFRGS, Porto Alegre, RS, Brazil. Electronic address:

Acanthamoeba castellanii is a free-living organism widely distributed in the environment that may cause disease. This protozoan exists in two forms, an infective trophozoite and a dormant cyst. The trophozoites are able to cause keratitis and granulomatous amoebic encephalitis in humans. Read More

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October 2018
5 Reads

Relationship between antioxidant defense in Acanthamoeba spp. infected lungs and host immunological status.

Exp Parasitol 2018 Oct 7;193:58-65. Epub 2018 Sep 7.

Department of Biology and Medical Parasitology, Pomeranian Medical University in Szczecin, Poland. Electronic address:

The role of oxidative stress in the pathogenicity of acanthamoebiasis is an important aspect of the intricate and complex host-parasite relationship. The aim of this experimental study was to determine oxidative stress through the assessment of lipid peroxidation product (LPO) levels and antioxidant defense mechanism in Acanthamoeba spp. lung infections in immunocompetent and immunosuppressed hosts. Read More

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October 2018
2 Reads