4,602 results match your criteria APMIS : acta pathologica microbiologica et immunologica Scandinavica[Journal]


Toward liquid biopsies in cancer treatment: application of circulating tumor DNA.

APMIS 2019 Feb 19. Epub 2019 Feb 19.

Center for Genomic Medicine, Rigshospitalet, Copenhagen University, Copenhagen, Denmark.

Circulating tumor DNA (ctDNA) refers to the fraction of cell-free DNA in a patient's blood originating from tumor cells. Increased knowledge about tumor genomics, improvements in targeted therapies, and accompanying advances in DNA-sequencing technologies have increased the interest in using ctDNA as a minimally invasive tool in cancer diagnostics and treatment. Especially, early tumor detection including identification of minimal residual disease and stratification of adjuvant therapy are promising approaches. Read More

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http://dx.doi.org/10.1111/apm.12912DOI Listing
February 2019

Cystic fibrosis - an example of personalized and precision medicine.

APMIS 2019 Feb 14. Epub 2019 Feb 14.

Cystic Fibrosis Center, Rigshospitalet, Copenhagen, Denmark.

Cystic fibrosis (CF) is a severe, monogenic, autosomal recessive disease caused by mutations in the CFTR (cystic fibrosis transmembrane regulator) gene, where disturbed chloride and bicarbonate transportation in epithelial cells results in a multiorgan disease with primarily pulmonary infections and pancreatic insufficiency. In 1968, the Copenhagen CF Center was established, and centralized care of CF patients with monthly control was introduced. Close monitoring and treatment of Pseudomonas lung infection as well as segregation of patients with different infection status improved the clinical outcome as well as survival. Read More

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http://dx.doi.org/10.1111/apm.12915DOI Listing
February 2019

Immunogenicity of HPV and HBV vaccines: adjuvanticity of synthetic analogs of monophosphoryl lipid A combined with aluminum hydroxide.

APMIS 2019 Mar;127(3):150-157

Department of Cell Therapy and Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Monophosphoryl lipid A (MPL), a purified and detoxified product of lipopolysaccharide (LPS) of Salmonella minnesota R595, has been used as an adjuvant in different vaccines. In this study, the efficacy of human papillomaviruses (HPV) and hepatitis B virus (HBV) vaccines formulated with aluminum hydroxide combined with two different synthetic MPLs, 3D-(6-acyl)-PHAD or 3D-PHAD, or aluminum hydroxide combined with the mixtures of such MPLs, has been assessed. The immunogenicity in female BALB/c mice was verified by two intramuscular injections of differently formulated HPV and HBV vaccines and the total immunoglobulin G (IgG) antibody response was considered to compare the employed adjuvants. Read More

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http://doi.wiley.com/10.1111/apm.12927
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http://dx.doi.org/10.1111/apm.12927DOI Listing
March 2019
1 Read

Chlamydia pneumoniae enhances Interleukin 8 (IL-8) production with reduced azithromycin sensitivity under hypoxia.

APMIS 2019 Mar;127(3):131-138

Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.

Obligate intracellular bacterium Chlamydia pneumoniae causes respiratory tract infections such as community-acquired pneumonia. During infection, C. pneumoniae induces inflammatory responses in host cells and the oxygen concentration at the infection sites subsequently decreases. Read More

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http://dx.doi.org/10.1111/apm.12924DOI Listing
March 2019
2 Reads

Autoantibodies to a specific peptide epitope of human Hsp60 (ATVLA) with homology to Helicobacter pylori HspB in H. pylori-infected patients.

APMIS 2019 Mar;127(3):139-149

Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.

Helicobacter pylori (Hp) may initiate autoimmunity as a result of molecular mimicry. The aim of this study was to compare the level of IgG antibodies to a specific epitope (P1 peptide) of human heat shock protein (Hsp)60 homologous to Hp Hsp60 (HspB) in the sera of healthy donors (HD), patients with Hp-related gastritis or coronary heart disease (CHD), uninfected or with Hp infection confirmed by rapid urease test, histological examination (dyspeptic patients) the C urea breath test ( C UBT), and anti-Hp antibodies (healthy donors, CHD patients). The Anti-P1 IgG induction by Hp was verified by adsorption of sera with these bacteria and by experimental immunization of Caviae porcellus with Hp. Read More

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http://doi.wiley.com/10.1111/apm.12925
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http://dx.doi.org/10.1111/apm.12925DOI Listing
March 2019
4 Reads

Biomarkers in tumors of the central nervous system - a review.

APMIS 2019 Feb 10. Epub 2019 Feb 10.

Department of Genetics and Pathology, Laboratory Medicine, Lund, Sweden.

Until recently, diagnostics of brain tumors were almost solely based on morphology and immunohistochemical stainings for relatively unspecific lineage markers. Although certain molecular markers have been known for longer than a decade (combined loss of chromosome 1p and 19q in oligodendrogliomas), molecular biomarkers were not included in the WHO scheme until 2016. Now, the classification of diffuse gliomas rests on an integration of morphology and molecular results. Read More

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http://dx.doi.org/10.1111/apm.12916DOI Listing
February 2019

The role of Tenascin-C and Twist1 in gastric cancer: cancer progression and prognosis.

APMIS 2019 Feb;127(2):64-71

Key Laboratory of Natural Resources of the Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University, Yanji, China.

The aim of the present study was to identify the relation between Tenascin-C (TNC) and Twist1 and determine their clinical significance in gastric cancer (GC). We analyzed the expression of TNC and Twist1 in 159 GC samples and in 91 non-tumor samples using immunohistochemistry. In this study, TNC expression in stromal fibroblasts of GC was remarkably higher than non-tumor gastric lesions. Read More

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http://doi.wiley.com/10.1111/apm.12919
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http://dx.doi.org/10.1111/apm.12919DOI Listing
February 2019
3 Reads

Carbamoyl phosphate synthetase 1 (CPS1) as a prognostic marker in chronic hepatitis C infection.

APMIS 2019 Feb;127(2):93-105

Hepatogastroenterology Department, Theodor Bilharz Research Institute, Giza, Egypt.

This study aims to assess the value of carbamoyl phosphate synthetase 1 (CPS1), as a non-invasive serum marker, for the evolution of chronic HCV infection and hepatic fibrosis. Seventy-two patients with HCV positive serum RNA and 15 health volunteers were enrolled in this study. Out of 72 patients, 10 patients had decompensated liver with ascites. Read More

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http://doi.wiley.com/10.1111/apm.12917
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http://dx.doi.org/10.1111/apm.12917DOI Listing
February 2019
7 Reads

Establishment and effects of allograft and synthetic bone graft substitute treatment of a critical size metaphyseal bone defect model in the sheep femur.

APMIS 2019 Feb;127(2):53-63

BONESUPPORT AB, Lund, Sweden.

Assessment of bone graft material efficacy is difficult in humans, since invasive methods like staged CT scans or biopsies are ethically unjustifiable. Therefore, we developed a novel large animal model for the verification of a potential transformation of synthetic bone graft substitutes into vital bone. The model combines multiple imaging methods with corresponding histology in standardized critical sized cancellous bone defect. Read More

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http://doi.wiley.com/10.1111/apm.12918
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http://dx.doi.org/10.1111/apm.12918DOI Listing
February 2019
4 Reads

Homologous role of CovRS two-component regulatory system in NAD -glycohydrolase activity in Streptococcus dysgalactiae subsp. equisimilis as in Streptococcus pyogenes.

APMIS 2019 Feb;127(2):87-92

Department of Bacteriology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.

Streptococcal toxic shock syndrome (STSS) is primarily caused by Streptococcus pyogenes, but it may also be caused by Streptococcus dysgalactiae subsp. equisimilis (SDSE). The analyses of S. Read More

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http://dx.doi.org/10.1111/apm.12914DOI Listing
February 2019
1 Read

GB virus type C cross-reactivity in clinical samples with a low hepatitis C virus antibody positive response.

APMIS 2019 02;127(2):109-111

Lab of Virology, National Institute for Infectious Diseases "Lazzaro Spallanzani" IRCCS, Rome.

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http://dx.doi.org/10.1111/apm.12921DOI Listing
February 2019
1 Read

Whole genome sequencing of breast cancer.

APMIS 2019 Jan 28. Epub 2019 Jan 28.

Centre for Genomic Medicine, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark.

Breast cancer was the first to take advantage of targeted therapy using endocrine therapy, and for up to 20% of all breast cancer patients a further significant improvement has been obtained by HER2-targeted therapy. Greater insight in precision medicine is to some extent driven by technical and computational progress, with the first wave of a true technical advancement being the application of transcriptomic analysis. Molecular subtyping further improved our understanding of breast cancer biology and has through a new tumor classification enabled allocation of personalized treatment regimens. Read More

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http://doi.wiley.com/10.1111/apm.12920
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http://dx.doi.org/10.1111/apm.12920DOI Listing
January 2019
2 Reads

Amino acid substitution mutations and mRNA expression levels of the pbp5 gene in clinical Enterococcus faecium isolates conferring high level ampicillin resistance.

APMIS 2019 Mar 27;127(3):115-122. Epub 2019 Jan 27.

Faculty of Medicine, Microbiology Section, Bam University of Medical Sciences, Bam, Iran.

In this study, clinical ampicillin-resistant Enterococcus faecium isolates with minimum inhibitory concentrations (MICs) for ampicillin in the ranges from 128 to ˃512 μg/mL (n = 17) and two ampicillin-susceptible isolates (MIC 1 μg/mL) were investigated. No β-lactamase production was detected in these isolates. Alterations in the C-terminal part of pbp5 and levels of pbp5 mRNA expression were investigated by sequencing and quantitative real-time qRT-PCR, respectively. Read More

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http://dx.doi.org/10.1111/apm.12922DOI Listing

Mapping bacterial biofilms on recovered orthopaedic implants by a novel agar candle dip method.

APMIS 2019 Mar 27;127(3):123-130. Epub 2019 Jan 27.

Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, USA.

While the detrimental effects of periprosthetic joint infections (PJIs) are well known, the process of biofilm formation on orthopaedic hardware is unclear. Previous work has shown that encasement of explant hardware in agar can aid in identifying biofilms. This study tested the utility of agar 'candle dip' method in detecting and mapping the location of biofilm on infected orthopedic components. Read More

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http://doi.wiley.com/10.1111/apm.12923
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http://dx.doi.org/10.1111/apm.12923DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6372321PMC
March 2019
4 Reads
1.922 Impact Factor

The inflamed sputum in lower respiratory tract infection: l-lactate levels are correlated to neutrophil accumulation.

APMIS 2019 Feb 6;127(2):72-79. Epub 2019 Jan 6.

Department of Pulmonary and Infectious Diseases, Nordsjaellands Hospital, Hillerød, Denmark.

Lower respiratory tract infections (LRTI) are common, but little is known about the response of biomarkers of inflammation in the lungs. Therefore, our primary aim was to compare the concentration of l-lactate to the concentration of neutrophils in sputum and systemic markers of infection. Because it is difficult to differentiate viral and bacterial infection on the basis of clinical presentation in LRTI, our secondary aim was to evaluate if l- and d-lactate may serve as markers of local inflammation as representatives of neutrophils and bacteria, respectively. Read More

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http://dx.doi.org/10.1111/apm.12913DOI Listing
February 2019
1 Read

Mycobacterium brisbanense lung infection facilitated by steroid induced adrenal insufficiency.

APMIS 2019 Feb 25;127(2):106-108. Epub 2018 Dec 25.

Department of Infectious Diseases, Rigshospitalet, Copenhagen, Denmark.

Mycobacterium brisbanense is rapid-growing nontuberculous mycobacteria. It was first described in 2004 as a human pathogen and only one case report has previously been published. We report a case of M. Read More

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http://dx.doi.org/10.1111/apm.12911DOI Listing
February 2019
1 Read

Accurate prediction of anti-phagocytic activity of Vibrio vulnificus by measurement of bacterial adherence to hydrocarbonsPrediction of Anti-Phagocytic Activity.

APMIS 2019 Feb 21;127(2):80-86. Epub 2018 Dec 21.

Laboratory of Veterinary Public Health, School of Veterinary Medicine, Kitasato University, Towada, Aomori, Japan.

Vibrio vulnificus can cause necrotizing soft tissue infection via exposure through an open wound, and the incubation period in cases of wound infection is only about 16 h. These facts strongly suggest that mechanisms to evade innate immune cell phagocytosis are essential for its pathogenicity. Hydrophobic interaction is one of the binding mechanisms between bacteria and phagocytes. Read More

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http://doi.wiley.com/10.1111/apm.12910
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http://dx.doi.org/10.1111/apm.12910DOI Listing
February 2019
7 Reads

Primary signet-ring stromal tumour of the testis: case report with literature review.

APMIS 2019 Jan;127(1):45-49

Institute of Pathology, University of Göttingen, Göttingen, Germany.

Primary signet-ring stromal tumour of the testis (PSRSTT) is a very rare type of primary testicular tumour, which is not actually mentioned in the current WHO categorization of tumours of the male genital organs. In this case we report about histologically and immunohistochemically features of this rare tumour. In the present case, PSRTT was found in the orchiectomy preparation of a 69-year-old man with painless swelling of his left testicle. Read More

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http://dx.doi.org/10.1111/apm.12907DOI Listing
January 2019
4 Reads

The diabetic phenotype is preserved in myotubes established from type 2 diabetic subjects: a critical appraisal.

Authors:
Michael Gaster

APMIS 2019 Jan;127(1):3-26

Laboratory for Molecular Physiology, Department of Pathology and Department of Endocrinology, Odense University Hospital, Odense, Denmark.

Cultured human myotubes offer a unique model to distinguish between primary and environmental factors in the aetiology of insulin resistance in human skeletal muscle. The objective of this review was to summarize our and other group studies on insulin resistance in human myotubes established from lean, obese and type 2 diabetes (T2D) subjects. Overall, studies of human myotubes established from lean, obese and T2D subjects clearly show that part of the diabetic phenotype observed in vivo is preserved in diabetic myotubes. Read More

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http://dx.doi.org/10.1111/apm.12908DOI Listing
January 2019
1 Read

Subtyping of intraductal papillary mucinous neoplasms - pitfalls of MUC1 immunohistochemistry.

APMIS 2019 Jan 22;127(1):27-32. Epub 2018 Nov 22.

Department of Pathology, Copenhagen University Hospital Rigshospitalet, København, Denmark.

Intraductal papillary mucinous neoplasms (IPMNs) are precursor lesions of pancreatic ductal adenocarcinoma (PDAC). Current edition of WHO Classification of Tumors of the Digestive System recognizes four different subtypes (gastric, intestinal, pancreatobiliary, and oncocytic) and recommends analysis of mucin expression (MUC1, MUC2, MUC5AC, MUC6) as well as evaluation of architectural and cell differentiation patterns for correct classification. However, there is no consensus on MUC1 expression of IPMN-lesions in the literature. Read More

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http://dx.doi.org/10.1111/apm.12900DOI Listing
January 2019
3 Reads

Comparison of methods for measuring antibiotic consumption in an intensive care unit.

APMIS 2019 Jan;127(1):33-40

Department of Neuroanaesthesiology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.

Hospitals worldwide are working on minimizing unnecessary use of antimicrobials. To assess actual changes of antimicrobial usage, correct and precise measurements are necessary. This study aimed to compare data on the purchase of antibiotics from the pharmacy and the administration of antibiotics to patients, respectively, in an intensive care unit (ICU). Read More

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http://dx.doi.org/10.1111/apm.12909DOI Listing
January 2019
1 Read

Human native endocarditis caused by Streptococcus canis-a case report.

APMIS 2019 Jan;127(1):41-44

Centre for Cardiovascular Surgery and Transplantation, Brno, Czech Republic.

We report a very rare case of Streptococcus canis native infective endocarditis in a 73-year-old woman living in close contact with her dog. Her echocardiography showed large calcifications in the mitral annulus, massive regurgitation below the posterior leaflet, and adjacent vegetation. Blood culture was positive for Streptococcus Lancefield group G. Read More

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http://dx.doi.org/10.1111/apm.12905DOI Listing
January 2019
3 Reads

WHO laboratory validation of Xpert CT/NG and Xpert TV on the GeneXpert system verifies high performances.

APMIS 2018 Dec;126(12):907-912

WHO Collaborating Centre for Gonorrhoea and Other Sexually Transmitted Infections, National Reference Laboratory for Sexually Transmitted Infections, Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.

Effective tests for diagnosis of sexually transmitted infections (STIs), used point of care to inform treatment and management decisions, are urgently needed. We evaluated the analytical sensitivity and specificity of the Xpert CT/NG and Xpert TV tests, examining 339 samples spiked with phenotypically and/or genetically diverse strains of Neisseria gonorrhoeae, Chlamydia trachomatis, and Trichomonas vaginalis, and other related species that may cross-react. The APTIMA Combo 2 test and APTIMA TV test were used as reference tests. Read More

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http://doi.wiley.com/10.1111/apm.12902
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http://dx.doi.org/10.1111/apm.12902DOI Listing
December 2018
7 Reads

The importance of early diagnosis of Mycobacterium abscessus complex in patients with cystic fibrosis.

APMIS 2018 Dec;126(12):885-891

Cystic Fibrosis Center Copenhagen, Rigshospitalet, Copenhagen, Denmark.

Mycobacterium abscessus complex can cause severe lung infections and has proven to be a serious threat to patients with cystic fibrosis and a challenge for clinicians due to difficulties in timely diagnosis and complex multidrug treatment regimes. Mycobacterial culture is the gold standard for diagnosis, but in most cystic fibrosis centers it is performed less frequently than culture for other pathogens. Consensus today recommends just one annual mycobacterial culture for asymptomatic patients with cystic fibrosis, a strategy likely to lead to diagnostic delays. Read More

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http://dx.doi.org/10.1111/apm.12903DOI Listing
December 2018
9 Reads

Autotaxin and vascular endothelial growth factor receptor-2 and -3 are related to vascular development during the progression of chronic viral hepatitis C.

APMIS 2018 Dec;126(12):913-921

Organized Center of Clinical Medicine, International University of Health and Welfare, Tokyo, Japan.

Vascular endothelial growth factor (VEGF) and autotaxin (ATX) play important roles in embryonic vasculogenesis and cancer progression. This study examines whether these two angiogenic factors cooperate in the mechanism that regulates vascular development during the progression of chronic viral hepatitis C (CVH-C) (Inuyama classification, F1-F4). First, surgical wedge biopsy specimens and needle biopsy specimens were obtained. Read More

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http://doi.wiley.com/10.1111/apm.12904
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http://dx.doi.org/10.1111/apm.12904DOI Listing
December 2018
9 Reads

Early depletion of contact system in patients with sepsis: a prospective matched control observational study.

APMIS 2018 Dec 5;126(12):892-898. Epub 2018 Nov 5.

Department of Clinical Sciences Lund, Anaesthesiology and Intensive Care, Lund University, Skane University Hospital, Lund, Sweden.

Activation of the contact system generates bradykinin from high-molecular-weight kininogen and has been suggested to participate in the pathophysiology of sepsis. To test this, we prospectively measured bradykinin and high-molecular-weight kininogen levels in a cohort of sepsis patients requiring intensive care. From 29 patients meeting criteria for sepsis or septic shock according to Sepsis-3, blood was sampled within 24 h and on the fourth day following admittance to intensive care. Read More

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http://doi.wiley.com/10.1111/apm.12898
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http://dx.doi.org/10.1111/apm.12898DOI Listing
December 2018
7 Reads

Maternal and congenital cytomegalovirus infections in a population-based pregnancy cohort study.

APMIS 2018 Dec 30;126(12):899-906. Epub 2018 Oct 30.

Department of Microbiology, Oslo University Hospital, University of Oslo, Oslo, Norway.

CMV is the most common cause of congenital infection. During the past few decades, there has been a change in behaviour that possibly has affected the CMV infection rate of mother and child. We investigated 1350 randomly selected pregnant women from the Norwegian Mother and Child Cohort Study using an algorithm for detection of maternal and congenital CMV (cCMV) infection including both serology and nucleic acid amplification assay. Read More

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http://doi.wiley.com/10.1111/apm.12899
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http://dx.doi.org/10.1111/apm.12899DOI Listing
December 2018
5 Reads

Toll-like receptors 2, 4 and 9 and hypoxia markers HIF-1alpha and CAIX in pancreatic intraepithelial neoplasia.

APMIS 2018 Nov;126(11):852-863

Cancer and Translational Medicine Research Unit, Medical Research Center Oulu, University of Oulu and Oulu University Hospital, Oulu, Finland.

Pancreatic cancer arises from precursor lesions called pancreatic intraepithelial neoplasia (PanIN) characterized by inflammatory microenvironment. In pancreatic cancer, strong innate immunity and hypoxia responses are typical. Occurrence and relationship of these responses in human PanINs is unknown. Read More

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http://doi.wiley.com/10.1111/apm.12894
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http://dx.doi.org/10.1111/apm.12894DOI Listing
November 2018
10 Reads

Bartonella quintana, past, present, and future of the scourge of World War I.

Authors:
Joaquim Ruiz

APMIS 2018 Nov;126(11):831-837

Independent Researcher, Barcelona, Spain.

During World War I, a mysterious new disease affected soldiers on both sides of battle field. The first reports described a relapsing fever of unknown origin with body lice being suggested as the vector. The outbreak affected >1 000 000 people, mostly soldiers fighting in front-line trenches. Read More

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http://doi.wiley.com/10.1111/apm.12895
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http://dx.doi.org/10.1111/apm.12895DOI Listing
November 2018
14 Reads

Replicon typing of plasmids in environmental Achromobacter sp. producing quinolone-resistant determinants.

APMIS 2018 Nov;126(11):864-869

Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (USP), Ribeirão Preto, Brazil.

This study aimed to investigate the antimicrobial resistance profile to quinolones, the presence of quinolone-resistant determinants and the plasmid replicon typing in environmental Achromobacter sp. isolated from Brazil. Soil and water samples were used for bacterial isolation. Read More

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http://dx.doi.org/10.1111/apm.12896DOI Listing
November 2018
3 Reads

A fatal case of enterovirus infection with secondary hemophagocytosis-case report with review of literature.

APMIS 2018 Nov;126(11):877-882

Department of Internal Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

Enterovirus is a common viral infection, which can affect multiple organ systems with an array of clinical presentation such as meningitis, encephalitis, myocarditis, and disseminated infections. The illness is usually asymptomatic and self-limited but few cases can be severe and life-threatening especially when associated with hemophagocytosis. We discuss a fatal case of disseminated enterovirus infection and the histomorphological features of the infection. Read More

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http://doi.wiley.com/10.1111/apm.12892
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http://dx.doi.org/10.1111/apm.12892DOI Listing
November 2018
19 Reads
1.922 Impact Factor

Rapid antimicrobial susceptibility testing of positive blood cultures by direct inoculation and reading of disc diffusion tests after 3-4 hours.

APMIS 2018 Nov;126(11):870-876

Department of Medical Microbiology, Vestre Viken Hospital Trust, Drammen, Norway.

The aim of the study was to validate whether rapid antimicrobial susceptibility testing (AST) by very early reading of disc diffusion tests would provide reliable results in daily routine work. A total of 264 positive blood culture bottles were examined, of which 178 were examined as part of the daily routine workflow. Enterobacterales were evaluated for resistance to cefotaxime, ceftazidime, gentamicin and ampicillin. Read More

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http://doi.wiley.com/10.1111/apm.12897
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http://dx.doi.org/10.1111/apm.12897DOI Listing
November 2018
6 Reads

Prevalence of CYP2D6*4 1934G/A polymorphism in Western Indian HIV patients.

APMIS 2018 Nov;126(11):842-851

Department of Clinical Sciences, National AIDS Research Institute, Pune, India.

Hepatic CYP2D6 enzyme metabolizes antiretroviral drugs (ARVs) including nevirapine. Polymorphism in CYP2D6 gene affects drug metabolism and displays distinctive phenotypes in the population. Hence, we investigated the prevalence of CYP2D6*4 1934G/A polymorphism in a total of 165 HIV patients that include 34 with and 131 without hepatotoxicity and 160 unrelated healthy controls by the PCR-RFLP method. Read More

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http://doi.wiley.com/10.1111/apm.12893
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http://dx.doi.org/10.1111/apm.12893DOI Listing
November 2018
2 Reads

Clinicopathological features of hydrophilic polymer emboli in Japanese autopsy cases.

APMIS 2018 Nov 15;126(11):838-841. Epub 2018 Oct 15.

Department of Pathology, Tokai University School of Medicine, Isehara, Japan.

Hydrophilic polymer is used on multiple endovascular devices to decrease friction between these devices and vessel walls. Although it can prevent medical device-related complications, hydrophilic polymer emboli (HPE) has recently been established as a potentially fatal iatrogenic phenomenon. HPE can cause tissue injury in numerous sites, including the brain, heart, lung, skin, and intestines. Read More

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http://doi.wiley.com/10.1111/apm.12891
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http://dx.doi.org/10.1111/apm.12891DOI Listing
November 2018
14 Reads

Medical and dental applications of nanomedicines.

APMIS 2018 Oct;126(10):795-803

Student Research Committee, Jahrom University of Medical Sciences, Jahrom, Iran.

Nanoparticles are tiny materials with nanosized components less than 100 nm in at least one dimension with physicochemical properties, which make them very attractive for medical application. These compounds have been evaluated as potential medicines for several decades. Nanotechnology has provided advances in the various fields of health sciences such as diagnosis, prevention and treatment by application of the agents named nanomedicines, including proteins, polymers, micelles, dendrimers, liposomes, emulsions, nanocapsules and nanoparticles. Read More

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http://dx.doi.org/10.1111/apm.12890DOI Listing
October 2018
16 Reads

Tumor microenvironmental factors have prognostic significances in advanced gastric cancer.

APMIS 2018 Oct;126(10):814-821

Department of Pathology, Nowon Eulji Medical Center, Eulji University, Seoul, Korea.

Tumor microenvironment is important in the progression and survival of cancer cells. We evaluated the prognostic significance of tumor stroma percentage (TSP), Klintrup-Mäkinen (KM) grade, which reflects the density of inflammatory cells of the tumor, and Glasgow microenvironment score (GMS), a combination of TSP and KM grade, in advanced gastric cancers. A total of 196 pT3 and pT4 gastric cancers were histologically evaluated using TSP, KM grade, and GMS. Read More

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http://doi.wiley.com/10.1111/apm.12889
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http://dx.doi.org/10.1111/apm.12889DOI Listing
October 2018
11 Reads

Predictive value of combined analysis of pro-NPY and ERG in localized prostate cancer.

APMIS 2018 Oct 6;126(10):804-813. Epub 2018 Sep 6.

Copenhagen Prostate Cancer Center, Department of Urology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.

This study aimed to investigate if combined analysis of pro-Neuropeptide Y (NPY) and ERG expression in tumor tissue are associated with biochemical failure (BF), castration-based treatment, castration-resistant prostate cancer (CRPC), and prostate cancer (PCa)-specific death for men undergoing radical prostatectomy (RP) for PCa. This study included 315 patients, who underwent RP from 2002 to 2005. Both pro-NPY and ERG expression were analyzed using immunohistochemistry and were scored as low or high and negative or positive, respectively. Read More

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http://dx.doi.org/10.1111/apm.12886DOI Listing
October 2018
2 Reads

Performance characteristics of newer MIC gradient strip tests compared with the Etest for antimicrobial susceptibility testing of Neisseria gonorrhoeae.

APMIS 2018 Oct 6;126(10):822-827. Epub 2018 Sep 6.

WHO Collaborating Centre for Gonorrhoea and Other STIs, Department of Laboratory Medicine, Microbiology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.

For Neisseria gonorrhoeae susceptibility testing, Etest, comparable to agar dilution, is frequently used. In recent years, newer MIC gradient strip tests have been commercialized. However, these tests have not been appropriately evaluated for gonococci. Read More

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http://doi.wiley.com/10.1111/apm.12887
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http://dx.doi.org/10.1111/apm.12887DOI Listing
October 2018
8 Reads
1.922 Impact Factor

The use of fluorescent staining techniques for microscopic investigation of polymorphonuclear leukocytes and bacteria.

APMIS 2018 Oct 6;126(10):779-794. Epub 2018 Sep 6.

Costerton Biofilm Center, Department of International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark.

The use of fluorescent stains to visually investigate eukaryotic and/or prokaryotic cells is increasing quickly and manuscripts within all areas of research publish results using fluorescent staining techniques. However, in contrast to literature on traditional histological staining techniques, the literature on fluorescent stains and staining techniques does not offer as good an illustration of cellular morphology. The aim of this guideline is to illustrate different fluorescent stains and staining techniques for imaging immune cells, in particular the polymorphonuclear leukocytes (PMNs), in combination with infecting bacteria as seen in chronic bacterial infections. Read More

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http://doi.wiley.com/10.1111/apm.12888
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http://dx.doi.org/10.1111/apm.12888DOI Listing
October 2018
5 Reads

Evaluation of the gastrointestinal tract in mdx mice: an experimental model of Duchenne muscular dystrophy.

APMIS 2018 Aug;126(8):693-699

Pharmacology Department, Faculdade de Medicina do ABC, Santo André, SP, Brazil.

This study describes the functional and morphological alterations in the intestines of mdx mice (n = 4) compared with the intestinal features of C57BL/10 mice (n = 7) at 2 months of age. The whole gut transit time (carmine red) and the upper gut transit time (activated charcoal) were measured, and light microscopy was utilized to view stained sections (H&E and picrosirius red) for histological analysis. No significant difference in mean evacuation time for the whole gut was observed between the two groups, but a significant delay in activated charcoal passage was observed in the mdx mice. Read More

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http://doi.wiley.com/10.1111/apm.12864
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http://dx.doi.org/10.1111/apm.12864DOI Listing
August 2018
10 Reads

Porphyromonas gingivalis-induced inflammatory responses in THP1 cells are altered by native and modified low-density lipoproteins in a strain-dependent manner.

APMIS 2018 Aug;126(8):667-677

Department of Medical Sciences, Örebro University, Örebro, Sweden.

Strong epidemiological evidence supports an association between cardiovascular and periodontal disease and furthermore, the periodontopathogen Porphyromonas gingivalis has been identified in blood and from atheromatous plaques. Blood exposed to P. gingivalis shows an increased protein modification of low-density lipoprotein (LDL). Read More

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http://dx.doi.org/10.1111/apm.12860DOI Listing
August 2018
2 Reads

Staphylococcus epidermidis from prosthetic joint infections induces lower IL-1β release from human neutrophils than isolates from normal flora.

APMIS 2018 Aug;126(8):678-684

School of Medical Sciences, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.

The aim of this study was to test the hypothesis that Staphylococcus epidermidis isolated from prosthetic joint infections (PJIs) differs from S. epidermidis isolated from normal flora in terms of its capacity to induce activation of caspase-1 and release of IL-1β in human neutrophils. The amount of active caspase-1 was determined over 6 h by detecting Ac-YVAD-AMC fluorescence in human neutrophils incubated with S. Read More

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http://dx.doi.org/10.1111/apm.12861DOI Listing
August 2018
8 Reads

Patient characteristics of olfactory neuroblastoma: experience from a tertiary cancer centre 2000-2016 covering Eastern Denmark.

APMIS 2018 Aug;126(8):663-666

Department of Otorhinolaryngology, Head and Neck Surgery and Audiology, Rigshospitalet, Copenhagen, Denmark.

The aim of this study was to report incidence and patient characteristics of olfactory neuroblastoma (ONB) at a tertiary cancer institution during a 16-year period. A retrospective review was conducted on patients with ONB treated at Rigshospitalet, Copenhagen from 2000 to 2016 covering Eastern Denmark. Patient demographics, symptoms, stage, pathology-reports, treatment, and outcome were extracted from the patient records and the Danish pathology register. Read More

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http://dx.doi.org/10.1111/apm.12859DOI Listing
August 2018
11 Reads

Detection of extended-spectrum beta-lactamase production in Enterobacteriales from patients with suspected urinary tract infections, Tabora region, Rural Tanzania.

APMIS 2018 08;126(8):700-702

Department of Laboratory Medicine, Clinical Microbiology, Faculty of Medicine and Health, Örebro University Hospital, Örebro University, Örebro, Sweden.

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http://dx.doi.org/10.1111/apm.12863DOI Listing
August 2018
1 Read

Primary and secondary mucosal melanoma of the small intestine - a clinical, pathological, and genetic nationwide survey of Danish patients between 1980 and 2014.

APMIS 2018 Sep;126(9):739-745

Department of Pathology, Rigshospitalet, Copenhagen, Denmark.

The aim of this study was to describe the epidemiology, symptomatology, pathology, genetics, and treatment of primary and metastatic small intestine melanoma in a national Danish cohort. All Danish patients diagnosed with small intestinal melanoma during the period 1980-2014 were included. For each patient, clinical data along with available pathology report and tissue was registered. Read More

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http://doi.wiley.com/10.1111/apm.12883
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http://dx.doi.org/10.1111/apm.12883DOI Listing
September 2018
12 Reads

Hypochromatic large urothelial cells in urine cytology are indicative of high grade urothelial carcinoma.

APMIS 2018 Sep;126(9):705-709

Faculty of Medicine, Unit of Anatomical Pathology, Cordoba University, Cordoba, Spain.

In this study, we have examined 67 cytology specimens from patients from 2014 to 2016. The ratio man to women was 4:1 with a median age of 75 years (range: 55-87 years). Thin-Prep processed urinary cytology specimens demonstrated large urothelial cells, with cytologic features of malignancy, thus including hypochromatic nuclei with occasional peripherally accentuated chromatin rim. Read More

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http://dx.doi.org/10.1111/apm.12877DOI Listing
September 2018
2 Reads

Stability of oestrogen and progesterone receptor antigenicity in formalin-fixed paraffin-embedded breast cancer tissue over time.

APMIS 2018 Sep;126(9):746-754

Division of Oncology and Pathology, Department of Clinical Sciences Lund, Skane University Hospital, Lund University, Lund, Sweden.

Use of archived formalin-fixed paraffin-embedded (FFPE) tissue is a standard method for evaluation of proposed prognostic and predictive tumour markers. However, little is known of the preservation of biomarker expression in old FFPE tumour blocks. We investigate the quality of immunohistochemical (IHC) oestrogen (ER) and progesterone receptor (PR) evaluation in FFPE tissue over time (1978-2000) using a large breast cancer tissue microarray (N = 573) with access to receptor analyses in cytosol (CYT) at diagnosis, coexpression of other biomarkers and follow-up data. Read More

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http://dx.doi.org/10.1111/apm.12884DOI Listing
September 2018
13 Reads
1.922 Impact Factor

Increased levels of serum miR-148a-3p are associated with prostate cancer.

APMIS 2018 Sep;126(9):722-731

St. Olavs Hospital, Trondheim University Hospital, Trondheim, Norway.

Prostate cancer (PCa) is one of the most common types of cancer and the fifth leading cause of death among men worldwide. The tools for diagnosing PCa have limited value, and to improve correct diagnosis there is a need for markers that can contribute to a more precise diagnosis, which would lead to proper treatment of only those patients who need it. Micro RNA (miRNA) plays a key role in the development of cancer and is therefore a potential marker for PCa. Read More

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http://dx.doi.org/10.1111/apm.12880DOI Listing
September 2018
14 Reads

Vascular adhesion protein-1 as indicator of breast cancer tumor aggressiveness and invasiveness.

APMIS 2018 Sep;126(9):755-761

Department of Pathology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.

Recent studies suggest that vascular adhesion protein-1 (VAP-1), a 180-KDa homodimeric glycoprotein, may be associated with cancer-related events including tumor cell migration, motility, invasion, or metastasis. Therefore, this study applies VAP-1 immunohistochemical staining to demonstrate the invasiveness component of the breast cancer. The VAP-1 staining results were compared in 148 breast cancer cases to identify possible correlations with clinical status, including age, tumor size, tumor grade, TNM stage, lymphatic invasion, metastasis, recurrence, and survival rate. Read More

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http://dx.doi.org/10.1111/apm.12885DOI Listing
September 2018
2 Reads

CD8+ and CD163+ infiltrating cells and PD-L1 immunoexpression in oral leukoplakia and oral carcinoma.

APMIS 2018 Sep;126(9):732-738

Department of Pathomorphology, Medical University of Lodz, Lodz, Poland.

Overexpression of inhibitory checkpoint PD1/PD-L1 plays an important role in carcinogenesis and patients prognosis. 70 cases of oral squamous cell carcinoma (OSCC), 23 cases of oral leukoplakia (OLK), and 19 control cases were immunohistochemically stained with anti-PD-L1, -CD8, and -CD163 antibodies. PD-L1 was expressed on dysplastic and subepithelial infiltrating cells of OLK as well as on cancer and tumor-infiltrating cells of OSCC. Read More

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http://doi.wiley.com/10.1111/apm.12881
Publisher Site
http://dx.doi.org/10.1111/apm.12881DOI Listing
September 2018
20 Reads