48,912 results match your criteria ALA Dehydratase Deficiency Porphyria


Characterization of interaction and ubiquitination of phosphoenolpyruvate carboxykinase by E3 ligase UBR5.

Biol Open 2018 Dec 14;7(12). Epub 2018 Dec 14.

Department of Biochemical and Molecular Sciences, Queen's University, Kingston, Ontario, K7L 3N6, Canada

Phosphoenolpyruvate carboxykinase (PEPCK1) is ubiquitinated by E3 ubiquitin ligase UBR5, which was thought to be facilitated by the acetylation of Lys70, Lys71 and Lys594 in PEPCK1. Here, we made a series of UBR5 HECT domain truncation variants and, through pull-down assay, showed that the N-terminal lobe of the UBR5 HECT domain is largely responsible for interacting with PEPCK1. We mutated all three lysine residues thought to be acetylated in PEPCK1 but were surprised to observe no loss of binding to UBR5 HECT domain. Read More

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December 2018

Alanine-Scanning Mutagenesis of α-Conotoxin GI Reveals the Residues Crucial for Activity at the Muscle Acetylcholine Receptor.

Mar Drugs 2018 Dec 13;16(12). Epub 2018 Dec 13.

Key Laboratory of Tropical Biological Resources, Ministry of Education, Key Lab for Marine Drugs of Haikou, Hainan University, Haikou 570228, Hainan, China.

Recently, the muscle-type nicotinic acetylcholine receptors (nAChRs) have been pursued as a potential target of several diseases, including myogenic disorders, muscle dystrophies and myasthenia gravis, etc. α-conotoxin GI isolated from selectively and potently inhibited the muscle-type nAChRs which can be developed as a tool to study them. Herein, alanine scanning mutagenesis was used to reveal the structure⁻activity relationship (SAR) between GI and mouse α1β1δε nAChRs. Read More

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December 2018
1 Read

Computational conformational analysis of α-thrombin inhibitors possessing distinct scaffolds in aqueous solution and on Ala-sheet.

Authors:
Keigo Gohda

J Mol Graph Model 2018 Dec 3;87:165-171. Epub 2018 Dec 3.

Computer-aided Molecular Modeling Research Center, Kansai (CAMM-Kansai), 3-32-302, Tsuto-Otsuka, Nishinomiya, 663-8241, Japan. Electronic address:

Recent computational simulations on protein-ligand binding/unbinding have precisely been uncovering the ligand-binding process at the atomic level. In the process, the non-specific binding of ligands to the target site is suggested to occur before binding to the target. We in this study analyzed the conformations of ligands under the non-specific binding on a protein surface to figure out the differences in the conformational characteristics in aqueous solution using the 55-ns molecular dynamic simulation. Read More

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December 2018

Cloning of carrier cells infected with oncolytic adenovirus driven by midkine promoter and biosafety studies.

J Gene Med 2018 Dec 13:e3064. Epub 2018 Dec 13.

Department of Obstetrics and Gynecology, School of Medicine, Ehime University, Ehime, Japan.

Background: A549 carrier cells infected with oncolytic adenovirus can induce complete tumor reduction of subcutaneous ovarian tumors but not intraperitoneal disseminated ovarian tumors. This seems to be due to the insufficient antitumor effect of A549 carrier cells. Therefore, here we cloned a novel carrier cell with the aim of improving antitumor effects. Read More

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December 2018
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Elucidation of the D-lysine biosynthetic pathway in the hyperthermophile Thermotoga maritima.

FEBS J 2018 Dec 7. Epub 2018 Dec 7.

Graduate School of Pharmaceutical Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, 108-8641, Japan.

Various D-amino acids are involved in peptidoglycan and biofilm metabolism in bacteria, suggesting that these compounds are necessary for successful adaptation to environmental changes. In addition to the conventional D-alanine (D-Ala) and D-glutamate, the peptidoglycan of the hyperthermophilic bacterium Thermotoga maritima contains both L-lysine (L-Lys) and D-Lys, but not meso-diaminopimelate (meso-Dpm). D-Lys is an uncommon component of peptidoglycan, and its biosynthetic pathway remains unclear. Read More

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December 2018

AuNP-peptide probe for caspase-3 detection in living cells by SERS.

Analyst 2018 Dec 14. Epub 2018 Dec 14.

State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, 300457 Tianjin, China. and Research Centre of Food Science and Human Health, School of Medicine, Nankai University, 300071 Tianjin, China.

Colloidal nanoparticles can be used as surface-enhanced Raman scattering (SERS) substrates because the very close spacing between particles existing in these colloidal systems is beneficial for the generation of extremely strong and highly spatially localized electric field enhancements. Herein, a caspase-3-specified peptide was used as a molecular cross-linker to engineer gold nanoparticle (AuNP) junctions in a controllable manner. The peptide was designed with a sequence of CCALNNPFFDVED (Cys-Cys-Ala-Leu-Asn-Asn-Pro-Phe-Phe-Asp-Val-Glu-Asp) or CCALNNKYDDVED (Cys-Cys-Ala-Leu-Asn-Asn-Lys-Tyr-Asp-Asp-Val-Glu-Asp), where the CALNN (Cys-Ala-Leu-Asn-Asn) fragment helps to stabilize AuNP suspension in aqueous media and the sequence of DVED (Asp-Glu-Val-Asp) can be cleaved by caspase-3. Read More

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December 2018
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Fatty acids characterization and oxidative stability of spray dried designer egg powder.

Lipids Health Dis 2018 Dec 13;17(1):282. Epub 2018 Dec 13.

Department of Chemistry, Faculty of Physical Sciences, Government College University, Faisalabad, Pakistan.

Background: Designer eggs (DEs) have gained positive importance in maintaining cholesterol level, triglyceride profile and protection towards cardiovascular diseases due to the presence of essential fatty acids (EFAs) such as omega-3 (or) n-3 fatty acids. However, extreme heat conditions effect the quality as well as quantity of EFAs during the production of designer egg dried powder (DEDP). Therefore, the main mandate of research was the development of DEDP and determination of spray drying conditions impact on fatty acids composition of DEDP samples. Read More

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December 2018
2.219 Impact Factor

Comparison of different treatment schemes in 5-ALA interstitial photodynamic therapy for high-grade glioma in a preclinical model: An MRI study.

Photodiagnosis Photodyn Ther 2018 Dec 10. Epub 2018 Dec 10.

Univ. Lille, INSERM, CHU Lille, U1189, ONCO-THAI, Image-Assisted Laser Therapy for Oncology, F-59000, Lille, France; Department of Neurosurgery, University Hospital, F-59000, Lille, France.

Background: There is currently no therapy that prevents high-grade glioma recurrence. Thus, these primary brain tumors have unfavorable outcomes. Recently, 5-ALA photodynamic therapy (PDT) has been proposed to delay relapse and is highly expected to have potential synergistic effects with the current standard of care. Read More

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December 2018

Electronic transport in a graphene single layer: application in amino acid sensing.

Phys Chem Chem Phys 2018 Dec 13. Epub 2018 Dec 13.

Facultad de Ingenieía, Universidad Nacional de Entre Ríos, 3101 Oro verde, Argentina.

We modeled a type of field-effect transistor device based on graphene for the recognition of amino acids with a potential application in the building of a protein sequencer. The theoretical model used was a combination of density functional theory (DFT) with the non-equilibrium Green's function (NEGF) in order to describe the coherent transport in molecular devices. First, we studied the physisorption of each amino acid on a graphene sheet and we reported the adsorption energy, the adsorption distances, the equilibrium configuration and the charge transfer of ten amino acids that can be considered as representative of all of the amino acids: histidine (His), alanine (Ala), aspartic acid (Asp), tyrosine (Tyr), arginine (Arg), glutamic acid (Glu), glycine (Gly), phenylalanine (Phe), proline (Pro) and lysine (Lys). Read More

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December 2018

Assessment of Lead Exposure and Urinary-δ-aminolevulinic Acid Levels in Male Lead Acid Battery Workers in Tamil Nadu, India.

J Health Pollut 2018 Mar 12;8(17):6-13. Epub 2018 Mar 12.

Regional Occupational Health Centre, (Southern) Bengaluru, Karnataka, India.

Background: Exposure to lead (Pb) affects multiple health outcomes and physiological systems. In adults, even small increases in blood Pb levels have been associated with decreased glomerular filtration rate, increased risk of hypertension and increased incidence of essential tremor. To date, there have been few Pb-exposure assessments using the United States Occupational Health and Safety Administration (OSHA) regulations. Read More

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5-Aminolevulinic Acid-Based Photodynamic Therapy Pretreatment Mitigates Ultraviolet A-Induced Oxidative Photodamage.

Oxid Med Cell Longev 2018 7;2018:9420745. Epub 2018 Nov 7.

Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.

Aim: To determine whether 5-aminolevulinic acid-based photodynamic therapy (ALA-PDT) is effective in combating ultraviolet A- (UVA-) induced oxidative photodamage of hairless mice skin and human epidermal keratinocytes .

Methods: In experiments, the human keratinocyte cell line (HaCaT cells) was divided into two groups: the experimental group was treated with ALA-PDT and the control group was left untreated. Then, the experimental group and the control group of cells were exposed to 10 J/m of UVA radiation. Read More

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November 2018

De-Novo Ligand Design against Mutated Huntington Gene by Ligand-Based Pharmacophore Modeling Approach.

Curr Comput Aided Drug Des 2018 Dec 6. Epub 2018 Dec 6.

Department of Mechanical Engineering (SMME), National University of Science and Technology, Islamabad . Pakistan.

Background: Huntington's disease is characterized by three side effects, including motor disturbances, psychiatric elements, and intellectual weakness. The onset for HD has nonlinear converse associations with the number of repeat sequences of the polyglutamine mutations, so that younger patients have a tendency for longer repeats length. This HD variation is because of a development of a polyglutamine (CAG) repeats in the exon 1 of the Huntingtin protein. Read More

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December 2018

Use of optical fluorescence agents during surgery for pituitary adenomas: current state of the field.

J Neurooncol 2018 Dec 6. Epub 2018 Dec 6.

Department of Neurological Surgery, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.

Introduction: Differentiation of normal pituitary from abnormal tumor tissue remains a surgical challenge despite improvements in optical visualization technology for pituitary adenoma (PA) surgery. During neurosurgical procedures for other tumor types, 5-aminolevulinic acid (5-ALA) has become a focus of investigation based on its high specificity in differentiating tumor tissue. However, the role of 5-ALA and other optical fluorescent agents in PA surgery remains less clear. Read More

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December 2018

Secondary Hyperparathyroidism in Patients with Biliopancreatic Diversion After 10 Years of Follow-up, and Relationship with Vitamin D and Serum Calcium.

Obes Surg 2018 Dec 6. Epub 2018 Dec 6.

Department of Endocrinology and Nutrition, Complejo Asistencial Universitario de León, Altos de Nava s/n, Edificio Administrativo Ala Sur, 2ª planta, 24008, León, Spain.

Background: Secondary hyperparathyroidism (SHPT) is a matter of concern after biliopancreatic diversion (BPD). The aim of this study was to investigate the relationship between SHPT, 25(OH)D, and calcium after BPD.

Design: A retrospective analysis in obese patients after BPD performed between 1998 and 2016. Read More

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December 2018

Sonodynamic Therapy for Malignant Glioma Using 220-kHz Transcranial Magnetic Resonance Imaging-Guided Focused Ultrasound and 5-Aminolevulinic acid.

Ultrasound Med Biol 2018 Dec 3. Epub 2018 Dec 3.

Department of Neurosurgery, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.

Sonodynamic therapy (SDT) is used to treat various malignancies and can be applied to brain tumors using a transcranial magnetic resonance imaging-guided focused ultrasound (TcMRgFUS) device. This study investigated the efficacy of 220-kHz TcMRgFUS combined with 5-aminolevulinic acid (5-ALA) on malignant glioma in vitro and in vivo. F98 cells were irradiated with focused ultrasound (FUS) (4000 J, 20 W, 240 s, 100% duty cycle, target medium temperature <40°C) after treatment with 200 µg/mL 5-ALA, and cell viability and apoptosis were evaluated with the water-soluble tetrazolium-1 assay, triple fluorescent staining and Western blot analysis 20 h later. Read More

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December 2018

Potential effects of different natural antioxidants on inflammatory damage and oxidative-mediated hepatotoxicity induced by gold nanoparticles.

Int J Nanomedicine 2018 23;13:7931-7938. Epub 2018 Nov 23.

Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, Saudi Arabia,

Objective: The objective of this study was to verify and confirm the oxidative-mediated hepatotoxicity, inflammatory liver damage, and oxidative stress induced by intraperitoneal administration of gold nanoparticles (GNPs) in vivo; characterize the effect of different natural antioxidants on these hazardous changes; and finally choose the most powerful antioxidant among these different natural antioxidants.

Methods: Ten-nanometer GNPs were dissolved in aqueous solution of 0.01% concentration. Read More

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November 2018

Characteristics of interactions at protein segments without non-local intramolecular contacts in the Protein Data Bank.

PLoS One 2018 11;13(12):e0205052. Epub 2018 Dec 11.

School of Life Science and Technology, Tokyo Institute of Technology, Nagatsuda-cho, Midori-ku, Yokohama, Kanagawa, Japan.

The principle of three-dimensional protein structure formation is a long-standing conundrum in structural biology. A globular domain of a soluble protein is formed by a network of atomic contacts among amino acid residues, but regions without intramolecular non-local contacts are often observed in the protein structure, especially in loop, linker, and peripheral segments with secondary structures. Although these regions can play key roles for protein function as interfaces for intermolecular interactions, their nature remains unclear. Read More

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December 2018
4 Reads

Exogenous alpha lipoic acid can stimulate photosystem II activity and the gene expressions of carbon fixation and chlorophyll metabolism enzymes in maize seedlings under drought.

J Plant Physiol 2018 Nov 29;232:65-73. Epub 2018 Nov 29.

Department of Biology, Faculty of Science, Karadeniz Technical University, 61080, Trabzon, Turkey. Electronic address:

Protective compounds such as non-enzymatic antioxidants, osmolytes and signal molecules have been applied to plants exposed to various environmental stresses to increase their stress tolerance. However, there are not enough records about the response of plants to alpha lipoic acid (ALA) application with antioxidant properties. Therefore, this study was designed to evaluate the function of exogenous ALA on the photosynthetic performance of maize seedlings grown in hydroponic conditions under drought stress. Read More

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November 2018
1 Read

Engineering of multiple modular pathways for high-yield production of 5-aminolevulinic acid in Escherichia coli.

Bioresour Technol 2018 Dec 3;274:353-360. Epub 2018 Dec 3.

The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China; The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China. Electronic address:

5-aminolevulinic acid (ALA), an important precursor of tetrapyrroles, has various applications in medicine and agriculture fields. Several methods have been adopted to enhance ALA synthesis in our previous studies. In this study, systematic metabolic engineering strategies were implemented to further improve ALA production in Escherichia coli. Read More

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December 2018
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Aging and Hyperglycemia Intensify Dyslipidemia-Induced Oxidative Stress and Inflammation in Rats: Assessment of Restorative Potentials of ALA and EPA + DHA.

Inflammation 2018 Dec 10. Epub 2018 Dec 10.

Department of Biochemistry, CSIR-Central Food Technological Research Institute, Mysore, Karnataka, 570020, India.

Effect of aging and hyperglycemia on oxidative stress (OS) and inflammation in dyslipidemic conditions has not been elucidated. Hence, in this study, we assessed the implications of aging, hyperglycemia, and also the dietary effect of n-3 fatty acids (α-linolenic acid (ALA) and eicosapentaenoic acid (EPA) + docosahexaenoic acid (DHA)) on OS and inflammation in dyslipidemic rats. Dyslipidemia was induced in young and aged rats by feeding high-fat lard (HFL) diet. Read More

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December 2018
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Is 5-ALA fluorescence of cerebral metastases a prognostic factor for local recurrence and overall survival?

J Neurooncol 2018 Dec 10. Epub 2018 Dec 10.

Department of Neurosurgery, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Moorenstraße 5, 40225, Düsseldorf, Germany.

Background: 5-Aminolevulinic acid (5-ALA) fluorescence-guided resection technique was first introduced for malignant glioma. However, the impact of the 5-ALA fluorescence behaviour of cerebral metastases is still unclear. Aim of this study was to determine the impact of PpIX-fluorescence on the local progression-free and overall survival. Read More

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December 2018

α‑lipoic acid protects against carbon tetrachloride‑induced liver cirrhosis through the suppression of the TGF‑β/Smad3 pathway and autophagy.

Mol Med Rep 2018 Dec 4. Epub 2018 Dec 4.

Spleen, Stomach and Hepatobiliary Department, The First Affiliated Hospital, Henan University of Chinese Medicine, Zhengzhou, Henan 450004, P.R. China.

α‑lipoic acid (ALA) is a naturally occurring antioxidant with protective effects against various hepatic injuries. The aim of the present study was to investigate the mechanisms by which ALA protects the liver from carbon tetrachloride (CCl4)‑induced liver cirrhosis. The widely used liver cirrhosis rat model was established via an intraperitoneal injection of 2 mg/kg 50% CCl4, three times/week for 8 weeks. Read More

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December 2018

Polymorphism of mitochondrial tRNA genes associated with the number of pigs born alive.

J Anim Sci Biotechnol 2018 26;9:86. Epub 2018 Nov 26.

1National Engineering Laboratory for Animal Breeding; Ministry of Agricultural Key Laboratory of Animal Genetics, Breeding and Reproduction; College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China.

Background: Mutations in mitochondrial tRNA genes have been widely reported association with human reproductions. It is also important to explore the effect on the number of piglets born alive (NBA). Here, 1017 sows were used to investigate the association between polymorphisms in mitochondrial tRNA genes and NBA. Read More

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November 2018

Significant expansion and red-shifting of fluorescent protein chromophore determined through computational design and genetic code expansion.

Biophys Rep 2018 10;4(5):273-285. Epub 2018 Nov 10.

1Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.

Abstract: Fluorescent proteins (FPs) with emission wavelengths in the far-red and infrared regions of the spectrum provide powerful tools for deep-tissue and super-resolution imaging. The development of red-shifted FPs has evoked widespread interest and continuous engineering efforts. In this article, based on a computational design and genetic code expansion, we report a rational approach to significantly expand and red-shift the chromophore of green fluorescent protein (GFP). Read More

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November 2018

RIP1 and RIP3 mediate hemin-induced cell death in HT22 hippocampal neuronal cells.

Neuropsychiatr Dis Treat 2018 15;14:3111-3119. Epub 2018 Nov 15.

Department of Neurosurgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, Fujian, People's Republic of China,

Background: Intracerebral hemorrhage (ICH) is a devastating neurological injury associated with significant mortality. Necroptosis is a newly identified type of programmed necrosis initiated by the activation of tumor necrosis factor alpha. Evidences had demonstrated the importance of necroptosis in neuronal cell death. Read More

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November 2018
1 Read

DOTA-MGS5, a new cholecystokinin-2 receptor targeting peptide analog with optimized targeting profile for theranostic use.

J Nucl Med 2018 Dec 7. Epub 2018 Dec 7.

Department of Nuclear Medicine, Medical University of Innsbruck, Austria.

Molecular imaging and targeted radiotherapy (TRT) with radiolabeled cholecystokinin-2-receptor (CCK2R) targeting peptide probes holds high promise to improve the clinical management of patients with metastatic medullary thyroid carcinoma (MTC) and other CCK2R-expressing malignancies. Low stability and suboptimal targeting of currently available radiolabeled peptide analogs has prompted us to seek for new stabilization strategies. In this study we present a new minigastrin analog with site-specific C-terminal modifications showing a highly optimized targeting profile. Read More

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December 2018

Single cell analyses demonstrate that a heme - GATA1 feedback loop regulates red cell differentiation.

Blood 2018 Dec 10. Epub 2018 Dec 10.

Department of Medicine, Division of Hematology, University of Washington, Seattle, WA, United States;

Erythropoiesis is the complex, dynamic, and tightly-regulated process that generates all mature red blood cells. To understand this process, we mapped the developmental trajectories of progenitors from wildtype, erythropoietin-treated and -deleted mice at single cell resolution. Importantly, we linked the quantity of each cell's surface proteins to its total transcriptome, which is a novel method. Read More

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December 2018

Direct detection of cysteine peptidases for MALDI-TOF MS analysis using fluorogenic substrates.

Anal Biochem 2018 Dec 4. Epub 2018 Dec 4.

Department of Chemistry, Moscow State University, Moscow, 119991, Russia.

A method is described for the direct detection of unstable cysteine peptidase activity in polyacrylamide gels after native electrophoresis using new selective fluorogenic peptide substrates, pyroglutamyl-phenylalanyl-alanyl-4-amino-7-methylcoumaride (Glp-Phe-Ala-AMC) and pyroglutamyl-phenylalanyl-alanyl-4-amino-7-trifluoromethyl-coumaride (Glp-Phe-Ala-AFC). The detection limit of the model enzyme papain was 17 pmol (0.29 μg) for Glp-Phe-Ala-AMC and 43 pmol (0. Read More

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December 2018

Multicenter experience with endovascular treatment of aortic coarctation in adults.

J Vasc Surg 2018 Oct 24. Epub 2018 Oct 24.

Division of Pediatric Cardiology, Mayo Clinic, Rochester, Minn.

Objective: The objective of this study was to evaluate outcomes of endovascular treatment of aortic coarctation in adults.

Methods: Clinical data and imaging studies of 93 consecutive patients treated at nine institutions from 1999 to 2015 were reviewed. We included newly diagnosed aortic coarctation (NCO), recurrent coarctation, and aneurysmal/pseudoaneurysmal degeneration (ANE) after prior open surgical repair (OSR) of coarctation. Read More

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October 2018
1 Read
3.021 Impact Factor

Influence of multiple stents on periprocedural stroke after carotid artery stenting in the Carotid Revascularization Endarterectomy versus Stent Trial (CREST).

J Vasc Surg 2018 Dec 4. Epub 2018 Dec 4.

Department of Neurology, Mayo Clinic, Jacksonville, Fla. Electronic address:

Background: In the Carotid Revascularization Endarterectomy versus Stent Trial (CREST), carotid artery atherosclerotic lesion length and nature of the lesions were important factors that predicted the observed difference in stroke rates between carotid endarterectomy and carotid artery stenting (CAS). Additional patient-related factors influencing CAS outcomes in CREST included age and symptomatic status. The importance of the operator's proficiency and its influence on periprocedural complications have not been well defined. Read More

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December 2018

Egg quality, fatty acid composition and gastrointestinal morphology of layer hens fed whole flaxseed with enzyme supplementation.

Br Poult Sci 2018 Dec 10. Epub 2018 Dec 10.

a Department of Animal and Rangeland Sciences , Oregon State University , Corvallis , OR 97331.

Flaxseed is a rich source of α-linolenic acid (ALA, 18:3 n-3). Feeding flaxseed to hens can increase n-3 fatty acids (FA) in eggs. However, non-starch polysaccharides (NSP) in flaxseed decrease nutrient digestibility and can have a negative impact on egg n-3 FA incorporation. Read More

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December 2018

Functional Characterization of Peptide Transporters in Bovine Mammary Epithelial Cells.

J Agric Food Chem 2018 Dec 10. Epub 2018 Dec 10.

The objective of this study was to characterize expression profile, transport kinetics and regulation of peptide transporters in bovine mammary epithelial cells (BMECs). Quantitative reverse transcription real-time PCR, western blot and immunofluorescence staining were used to investigate the expression of peptide transporters in bovine mammary tissues. The effects of time, pH, concentration and specific inhibitors on β-alanyl-L-lysyl-Nε-7-amino-4-methyl-coumarin-3-acetic acid (β-Ala-Lys-AMCA) uptake in BMECs were also studied. Read More

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December 2018

Omega-3 fatty acids for the primary and secondary prevention of cardiovascular disease.

Cochrane Database Syst Rev 2018 11 30;11:CD003177. Epub 2018 Nov 30.

Norwich Medical School, University of East Anglia, Norwich Research Park, Norwich, Norfolk, UK, NR4 7TJ.

Background: Researchers have suggested that omega-3 polyunsaturated fatty acids from oily fish (long-chain omega-3 (LCn3), including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)), as well as from plants (alpha-linolenic acid (ALA)) benefit cardiovascular health. Guidelines recommend increasing omega-3-rich foods, and sometimes supplementation, but recent trials have not confirmed this.

Objectives: To assess effects of increased intake of fish- and plant-based omega-3 for all-cause mortality, cardiovascular (CVD) events, adiposity and lipids. Read More

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November 2018
9 Reads

The application of fluorescence techniques in meningioma surgery-a review.

Neurosurg Rev 2018 Dec 6. Epub 2018 Dec 6.

Department of Neurosurgery, University Medical Center Groningen, University of Groningen, Hanzeplein 1, P.O. Box 30.001, 9700 RB, Groningen, The Netherlands.

Surgical resections of meningiomas, the most common intracranial tumor in adults, can only be curative if radical resection is achieved. Potentially, the extent of resection could be improved, especially in complex and/or high-grade meningiomas by fluorescence-guided surgery using 5-aminolevulinic acid (5-ALA), indocyanine green (ICG), or fluorescein. This review aims to summarize and evaluate these fluorescence-guided meningioma surgery techniques. Read More

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December 2018
1 Read

Aromaticity, Coulomb repulsion, π delocalization or strain: who is who in endohedral metallofullerene stability?

Phys Chem Chem Phys 2018 Dec 6. Epub 2018 Dec 6.

Departamento de Química, Módulo 13, Universidad Autónoma de Madrid, 28049 Madrid, Spain. and Condensed Matter Physics Center (IFIMAC), Universidad Autónoma de Madrid, 28049 Madrid, Spain and Instituto Madrileño de Estudios Avanzados en Nanociencia (IMDEA-Nanociencia), Cantoblanco, 28049 Madrid, Spain and Donostia International Physics Center (DIPC), Paseo Manuel de Lardizabal 4, 20018 Donostia-San Sebastián, Spain.

Endohedral metallofullerenes (EMFs) synthetized in the laboratory are known to often violate the isolated pentagon and pentagon adjacency penalty rules that successfully describe the relative stability of pristine fullerene isomers. To explain these anomalies, several models have been proposed. In this work, we have systematically investigated the performance of the widely used IPSI (inverse pentagon separation index), ALA (additive local aromaticity) and CSI (charge stabilization index) models in predicting the relative stability of a large number of EMF isomers with cages ranging from C28 to C104 and charge states of 4- and 6-. Read More

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December 2018
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Efficacy of a repair cream containing Rhealba oat plantlets extract l-ALA-l-GLU dipeptide, and hyaluronic acid in wound healing following dermatological acts: a meta-analysis of >2,000 patients in eight countries corroborated by a dermatopediatric clinical case.

Clin Cosmet Investig Dermatol 2018 13;11:579-589. Epub 2018 Nov 13.

Pierre Fabre Dermo-Cosmétique, A-DERMA, Lavaur, France,

Background: The frequency of dermatological acts is increasing. These procedures often cause injuries and traumatic alterations in specific skin layers, slowing down wound healing.

Patients And Methods: An open observational study lasting 1 month was conducted on 2,363 patients who had undergone various dermatological procedures. Read More

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November 2018
4 Reads

Design of Linear and Cyclic Mutant Analogues of Dirucotide Peptide (MBP) against Multiple Sclerosis: Conformational and Binding Studies to MHC Class II.

Brain Sci 2018 Dec 4;8(12). Epub 2018 Dec 4.

Department of Chemistry, University of Patras, 26500 Patras, Greece.

Background: Multiple sclerosis (MS) is an autoimmune disorder of the central nervous system. MS is a T cell-mediated disease characterized by the proliferation, infiltration, and attack of the myelin sheath by immune cells. Previous studies have shown that cyclization provides molecules with strict conformation that could modulate the immune system. Read More

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December 2018
1 Read

Fabrication and characterization of the 3D-printed polycaprolactone/fish bone extract scaffolds for bone tissue regeneration.

J Biomed Mater Res B Appl Biomater 2018 Dec 3. Epub 2018 Dec 3.

Department of Biomedical Engineering, and Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan, Republic of Korea.

Fish bone extract (FBE) containing a trioligopeptide (FBP-KSA, Lys-Ser-Ala) isolated from Johnius belengerii could induce osteogenic activities on MC3T3-E1 pre-osteoblasts in our previous study. Regarding the osteogenic effect of FBE, in the present study, we fabricated the three-dimensional (3D) interconnected polycaprolactone (PCL)/FBE scaffolds for bone tissue regeneration. After fabrication of PCL scaffolds using 3D printing, FBE was coated on the surface of PCL scaffolds by self-assembly process. Read More

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December 2018
1 Read

Neurometabolite changes in patients with complex regional pain syndrome using magnetic resonance spectroscopy: a pilot study.

Neuroreport 2018 Nov 29. Epub 2018 Nov 29.

Emotional Information and Communication Technology Association, Seoul, Republic of Korea.

The aim of this study was to investigate distinct neurometabolites in the right and left thalamus and insula of patients with complex regional pain syndrome (CRPS) compared with healthy controls using proton magnetic resonance spectroscopy. Levels of N-acetylaspartate (NAA), N-acetylaspartylglutamate (NAAG), myo-inositol (ml), glutamine (Gln), glycerophosphocholine (GPC), glutathione (GSH), and alanine (Ala) relative to total creatine (tCr) levels, including creatine and phosphocreatine, were determined in the right and left thalamus and insula in 12 patients with CRPS compared with 11 healthy controls using magnetic resonance spectroscopy. Levels of NAAG/tCr and Ala/tCr were higher in patients with CRPS than in controls in the left thalamus. Read More

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November 2018
4 Reads
1.644 Impact Factor

Low-linoleic acid diet and oestrogen enhance the conversion of α-linolenic acid into DHA through modification of conversion enzymes and transcription factors.

Br J Nutr 2018 Dec 3:1-9. Epub 2018 Dec 3.

Department of Food and Nutrition,Hanyang University,222 Wangsimni-ro, Seongdong-gu, Seoul 04763,Republic of Korea.

Conversion of α-linolenic acid (ALA) into the longer chain n-3 PUFA has been suggested to be affected by the dietary intake of linoleic acid (LA), but the mechanism is not well known. Therefore, the purpose of this study was to evaluate the effect of a low-LA diet with and without oestrogen on the fatty acid conversion enzymes and transcription factors. Rats were fed a modified American Institute of Nutrition-93G diet with 0% n-3 PUFA or ALA, containing low or high amounts of LA for 12 weeks. Read More

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December 2018
2 Reads

From Kinetics of OH Reaction with Glutamic Acid to Oxidative Damage to Proteins.

J Phys Chem A 2018 Dec 3. Epub 2018 Dec 3.

Hydroxyl (OH) is a radical that is distributed all over the universe including human body, where it can be readily produced, results in oxidative damage to cellular components and leads to several health implications. While the reaction of OH with proteins is usually addressed as the gas phase reaction of free amino acids with OH in theoretical studies, this study questions the efficiency of such calculations by focusing on the reaction of glutamic acid (Glu) with OH. According to the results, the reaction profile alters significantly by shifting from gas phase calculations to the solvent phase due to the zwitterionic nature of this amino acid. Read More

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December 2018
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Preparation of α-Linolenic-Acid-Loaded Water-in-Oil-in-Water Microemulsion and Its Potential as a Fluorescent Delivery Carrier with a Free Label.

J Agric Food Chem 2018 Dec 3. Epub 2018 Dec 3.

School of Chemical Engineering and Technology , Tianjin University , Tianjin 300072 , People's Republic of China.

Our previous work has demonstrated that α-linolenic acid (ALA)-loaded oil-in-water (O/W) microemulsion could enhance ALA antioxidant capacity. Meanwhile, we also observed that synthesized microemulsion itself had fluorescence. In this work, we have prepared a multiple water-in-oil-in-water (W/O/W) microemulsion to further enhance ALA antioxidant capacity and activate this delivery carrier application potential with a free label. Read More

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December 2018
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Association of the human aryl hydrocarbon receptor repressor (AhRR)-c.565C>G transversion with male infertility: A case-control study from Iran.

J Cell Biochem 2018 Dec 2. Epub 2018 Dec 2.

Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran.

Dioxins (eg, 2,3,7,8-tetrachlorodibenzo-p-dioxin/TCDD), as environmental endocrine disruptors and toxic carcinogens, can affect male reproductive health. The influence of dioxins is mediated via the aryl hydrocarbon receptor (AhR) pathway and its repressor (AhRR). In this study, we investigated the association of AhRR-c. Read More

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December 2018
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3.263 Impact Factor

The impact of 5-aminolevulinic acid on extent of resection in newly diagnosed high grade gliomas: a systematic review and single institutional experience.

J Neurooncol 2018 Dec 1. Epub 2018 Dec 1.

Hermelin Brain Tumor Center, Department of Neurosurgery, Henry Ford Health System, Detroit, MI, USA.

Background: Glioma surgery at its nascency relied predominantly on visual and tactile feedback for the removal of grossly abnormal tissue. This technique has inherent limitations in delineating infiltrative tumor from normal brain, thus limiting the ability to achieve a gross total resection consistently. Since extent of resection (EOR) is consistently correlated with measures of survival, fluorescence-guided surgery shows promise in improving our ability to treat high-grade gliomas (HGG). Read More

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December 2018
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The effect of and α-linolenic acid on oxidant/antioxidant biomarkers of human peripheral blood mononuclear cells.

Indian J Pharmacol 2018 Jul-Aug;50(4):177-184

Department of Basic Science, School of Veterinary, Shiraz University, Shiraz, Iran.

Objectives: Various pharmacological effects including antioxidant property of L. were reported previously. In the present study, the effect of the extract of the plant and its constituent, α-linolenic acid (ALA), on oxidant and antioxidant markers of PHA/non-stimulated human mononuclear cells was evaluated. Read More

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December 2018
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Fluorescence assay to predict activity of the glycopeptide antibiotics.

J Antibiot (Tokyo) 2018 Nov 30. Epub 2018 Nov 30.

Institute of Microbiology v. v. i., Czech Academy of Sciences, Vestec, Czech Republic.

Here, we describe a fluorescent assay developed to study competitive binding of the glycopeptide antibiotics to live bacteria cells. This assay demonstrated that the mechanism of action of the lipoglycopeptide antibiotics strongly depends on the hydrophobicity of the substitutes, with the best antibacterial activity of the glycopeptide antibiotics equally sharing properties of binding to D-Ala-D-Ala residues of the nascent peptidoglycan and to the membrane. Read More

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November 2018
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5-aminolaevulinic acid (ALA), enhances heme oxygenase (HO)-1 expression and attenuates tubulointerstitial fibrosis and renal apoptosis in chronic cyclosporine nephropathy.

Biochem Biophys Res Commun 2018 Dec 1. Epub 2018 Dec 1.

Division of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, Japan. Electronic address:

Background: Cyclosporine-A (CsA) is an immunosuppressant indicated for various immunological diseases; however, it can induce chronic kidney injury. Oxidative stress and apoptosis play a crucial role in CsA-induced nephrotoxicity. The present study evaluated the protective effect of combining 5-aminolaevulinic acid with iron (5-ALA/SFC), a precursor of heme synthesis, to enhance HO-1 activity against CsA-induced chronic nephrotoxicity. Read More

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December 2018
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[Identification of a novel Ax allele of the ABO blood group].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2018 Dec;35(6):891-893

The First Clinical Medical College of Wenzhou Medical University, Wenzhou, Zhejiang 325035, China.

Objective: To explore the molecular basis for an individual with Ax28 phenotype of the ABO subtype.

Methods: The ABO group antigens on red blood cells of the proband were identified by monoclonal antibodies. The ABO antibody in serum was detected by standard A, B, O cells. Read More

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December 2018
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Dietary pattern regulates fatty acid desaturase 1 gene expression in Indian pregnant women to spare overall long chain polyunsaturated fatty acids levels.

Mol Biol Rep 2018 Dec 3. Epub 2018 Dec 3.

Division of Nutritional Sciences, Cornell University, Ithaca, NY, USA.

The aim of this study was to determine if the dietary pattern of pregnant women has any compensatory effect on the fatty acid desaturase (FADS) gene expression, thus enhancing the conversion of precursors to long chain polyunsaturated fatty acids (LCPUFA) to spare the overall LCPUFA levels. The dietary intake of plant-based precursor polyunsaturated fatty acids (PUFA) influences circulating levels of LCPUFA. We hypothesized that low LCPUFA diets during pregnancy would compensate by higher expression of FADS genes to enhance the conversion of precursors to LCPUFA to spare the overall LCPUFA levels. Read More

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December 2018
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PBP3 and PBP4 facilitate NOD1 agonist peptidoglycan fragment release and survival in stationary phase.

Infect Immun 2018 Dec 3. Epub 2018 Dec 3.

Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, Wisconsin, USA

releases peptidoglycan fragments during growth, and these molecules induce an inflammatory response in the human host. The proinflammatory molecules include peptidoglycan monomers, peptidoglycan dimers, and free peptides. These molecules can be released by the actions of lytic transglycosylases or an amidase. Read More

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December 2018
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