1,066 results match your criteria AJS; American journal of sociology[Journal]


Reflux symptoms and oesophageal acidification in treated achalasia patients are often not reflux related.

Gut 2020 May 21. Epub 2020 May 21.

Gastroenterology and Hepatology, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.

Objective: After treatment, achalasia patients often develop reflux symptoms. Aim of this case-control study was to investigate mechanisms underlying reflux symptoms in treated achalasia patients by analysing oesophageal function, acidification patterns and symptom perception.

Design: Forty treated achalasia patients (mean age 52. Read More

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http://dx.doi.org/10.1136/gutjnl-2020-320772DOI Listing

Spectrin couples cell shape, cortical tension, and Hippo signaling in retinal epithelial morphogenesis.

J Cell Biol 2020 Apr;219(4)

Department of Physiology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX.

Although extracellular force has a profound effect on cell shape, cytoskeleton tension, and cell proliferation through the Hippo signaling effector Yki/YAP/TAZ, how intracellular force regulates these processes remains poorly understood. Here, we report an essential role for spectrin in specifying cell shape by transmitting intracellular actomyosin force to cell membrane. While activation of myosin II in Drosophila melanogaster pupal retina leads to increased cortical tension, apical constriction, and Yki-mediated hyperplasia, spectrin mutant cells, despite showing myosin II activation and Yki-mediated hyperplasia, paradoxically display decreased cortical tension and expanded apical area. Read More

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http://dx.doi.org/10.1083/jcb.201907018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7147103PMC

Implementing receiver-driven handoffs to the emergency department to reduce miscommunication.

BMJ Qual Saf 2020 Apr 16. Epub 2020 Apr 16.

Department of Pediatrics, Boston Children's Hospital, Boston, Massachusetts, USA.

Background: Miscommunications during care transfers are a leading cause of medical errors. Recent consensus-based recommendations to standardise information transfer from outpatient clinics to the emergency department (ED) have not been formally evaluated. We sought to determine whether a receiver-driven structured handoff intervention is associated with 1) increased inclusion of standardised elements; 2) reduced miscommunications and 3) increased perceived quality, safety and efficiency. Read More

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http://dx.doi.org/10.1136/bmjqs-2019-010540DOI Listing

A Novel Pharmacological Approach to Enhance the Integrity and Accelerate Restitution of the Intestinal Epithelial Barrier.

Inflamm Bowel Dis 2020 Apr 8. Epub 2020 Apr 8.

Department of Inflammation and Immunity, Lerner Research Institute of Cleveland Clinic Foundation, Cleveland, OH.

Background: Disruption of the gut barrier is an essential mechanism of inflammatory bowel diseases (IBDs) contributing to the development of mucosal inflammation. A hallmark of barrier disruption is the disassembly of epithelial adherens junctions (AJs) driven by decreased expression of a major AJ protein, E-cadherin. A group of isoxazole compounds, such as E-cadherin-upregulator (ECU) and ML327, were previously shown to stimulate E-cadherin expression in poorly differentiated human cancer cells. Read More

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http://dx.doi.org/10.1093/ibd/izaa063DOI Listing

Use of real-world data for HPV vaccine trial follow-up in the Nordic region.

Contemp Clin Trials 2020 May 1;92:105996. Epub 2020 Apr 1.

Department of Research, Cancer Registry of Norway, Ullernchausseen 64, 0379 Oslo, Norway. Electronic address:

Post-marketing studies are commonly performed to follow-up on the safety and effectiveness of a drug or vaccine after approval has been obtained. These post-marketing studies may involve the collection of real-world data from registries and clinical biobanks in order to obtain real-world evidence. As this approach can monitor the effects of pharmaceutical products over decades, it is particularly necessary for the development of safe and effective vaccines. Read More

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http://dx.doi.org/10.1016/j.cct.2020.105996DOI Listing
May 2020
1.986 Impact Factor

Metformin inhibition of colorectal cancer cell migration is associated with rebuilt adherens junctions and FAK downregulation.

J Cell Physiol 2020 Apr 2. Epub 2020 Apr 2.

Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Inmunología, Genética y Metabolismo, Facultad de Farmacia y Bioquímica, Hospital de Clínicas "José de San Martín", Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina.

E-cadherin, a central component of the adherens junction (AJ), is a single-pass transmembrane protein that mediates cell-cell adhesion. The loss of E-cadherin surface expression, and therefore cell-cell adhesion, leads to increased cell migration and invasion. Treatment of colorectal cancer (CRC)-derived cells (SW-480 and HT-29) with 2. Read More

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http://dx.doi.org/10.1002/jcp.29677DOI Listing

Optimising subjective anterior eye grading precision.

Cont Lens Anterior Eye 2020 Mar 23. Epub 2020 Mar 23.

Ophthalmic Research Group, Aston University, Birmingham, UK. Electronic address:

Purpose: To establish the optimum grading increment which ensured parity between practitioners while maximising clinical precision.

Methods: Second year optometry students (n = 127, 19.5 ± 1. Read More

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http://dx.doi.org/10.1016/j.clae.2020.03.006DOI Listing

Loss of PP2A Disrupts the Retention of Radial Glial Progenitors in the Telencephalic Niche to Impair the Generation for Late-Born Neurons During Cortical Development†.

Cereb Cortex 2020 Mar 18. Epub 2020 Mar 18.

Department of Anesthesiology, The Second Affiliated Changzhou People's Hospital of Nanjing Medical University, Changzhou 213000, China.

Telencephalic radial glial progenitors (RGPs) are retained in the ventricular zone (VZ), the niche for neural stem cells during cortical development. However, the underlying mechanism is not well understood. To study whether protein phosphatase 2A (PP2A) may regulate the above process, we generate Ppp2cα conditional knockout (cKO) mice, in which PP2A catalytic subunit α (PP2Acα) is inactivated in neural progenitor cells in the dorsal telencephalon. Read More

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http://dx.doi.org/10.1093/cercor/bhaa042DOI Listing

Cervical Spondylotic Myelopathy: A Guide to Diagnosis and Management.

J Am Board Fam Med 2020 Mar-Apr;33(2):303-313

From the University of Miami Leonard M. Miller School of Medicine, Department of Education, Miami, FL (JRM, AJS, NCS); University of Miami Hospital, Department of Orthopaedic Surgery, Miami, FL (AJB); The Rothman Institute, Thomas Jefferson University Hospital, Philadelphia, PA (CJD).

Cervical spondylotic myelopathy (CSM) is a neurologic condition that develops insidiously over time as degenerative changes of the spine result in compression of the cord and nearby structures. It is the most common form of spinal cord injury in adults; yet, its diagnosis is often delayed. The purpose of this article is to review the pathophysiology, natural history, diagnosis, and management of CSM with a focus on the recommended timeline for physicians suspecting CSM to refer patients to a spine surgeon. Read More

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http://dx.doi.org/10.3122/jabfm.2020.02.190195DOI Listing

Early Events in Actin Cytoskeleton Dynamics and E-Cadherin-Mediated Cell-Cell Adhesion during Epithelial-Mesenchymal Transition.

Cells 2020 Feb 29;9(3). Epub 2020 Feb 29.

Institute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, 115478 Moscow, Russia.

Epithelial-mesenchymal transition (EMT) plays an important role in development and also in initiation of metastasis during cancer. Disruption of cell-cell contacts during EMT allowing cells to detach from and migrate away from their neighbors remains poorly understood. Using immunofluorescent staining and live-cell imaging, we analyzed early events during EMT induced by epidermal growth factor (EGF) in IAR-20 normal epithelial cells. Read More

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http://dx.doi.org/10.3390/cells9030578DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140442PMC
February 2020

Adherens Junctions: Guardians of Cortical Development.

Front Cell Dev Biol 2020 28;8. Epub 2020 Jan 28.

Queensland Brain Institute, The University of Queensland, Brisbane, QLD, Australia.

Apical radial glia comprise the pseudostratified neuroepithelium lining the embryonic lateral ventricles and give rise to the extensive repertoire of pyramidal neuronal subtypes of the neocortex. The establishment of a highly apicobasally polarized radial glial morphology is a mandatory prerequisite for cortical development as it governs neurogenesis, neural migration and the integrity of the ventricular wall. As in all epithelia, cadherin-based adherens junctions (AJs) play an obligate role in the maintenance of radial glial apicobasal polarity and neuroepithelial cohesion. Read More

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http://dx.doi.org/10.3389/fcell.2020.00006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7025593PMC
January 2020

Sensing Actin Dynamics through Adherens Junctions.

Cell Rep 2020 Feb;30(8):2820-2833.e3

Department of Dermatology, Northwestern University, The Feinberg School of Medicine, Chicago, IL 60611, USA. Electronic address:

We study punctate adherens junctions (pAJs) to determine how short-lived cadherin clusters and relatively stable actin bundles interact despite differences in dynamics. We show that pAJ-linked bundles consist of two distinct regions-the bundle stalk (AJ-BS) and a tip (AJ-BT) positioned between cadherin clusters and the stalk. The tip differs from the stalk in a number of ways: it is devoid of the actin-bundling protein calponin, and exhibits a much faster F-actin turnover rate. Read More

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http://dx.doi.org/10.1016/j.celrep.2020.01.106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7093076PMC
February 2020
7.207 Impact Factor

The extracellular and intracellular regions of Crb2a play distinct roles in guiding the formation of the apical zonula adherens.

Biomed Pharmacother 2020 May 7;125:109942. Epub 2020 Feb 7.

Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; The Institute of Translational Medicine, Zhejiang University, Hangzhou, 310058, China; Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou, 310009, China. Electronic address:

The transmembrane protein Crumbs (Crb), a key regulator of apical polarity, has a known involvement in establishment of the apical zonula adherens in epithelia, although the precise mechanism remains elusive. The zonula adherens are required to maintain the integrity and orderly arrangement of epithelia. Loss of the zonula adherens leads to morphogenetic defects in the tissues derived from epithelium. Read More

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http://dx.doi.org/10.1016/j.biopha.2020.109942DOI Listing

Secondary Analysis of Pain Outcomes in a Large Pragmatic Randomized Trial of Buprenorphine/Naloxone Versus Methadone for Opioid Use Disorder.

J Addict Med 2020 Feb 7. Epub 2020 Feb 7.

New York State Psychiatric Institute, New York, NY, (MS, SL, ANCC, JS, AB, EVN); Department of Psychiatry, Columbia University Medical Center, New York, NY, (MS, SL, ANCC, JS, EVN); Department of Biostatistics, Mailman School of Public Health, Columbia University, New York, NY, (MP); Department of Psychiatry and Behavioral Sciences, University of Washington School of Medicine; Center of Excellence in Substance Abuse Treatment and Education VA Puget Sound Health Care System, WA (AJS).

Objective: Opioid use disorder (OUD) is associated with chronic pain. We investigated the association between medication treatments for OUD and pain in a post-hoc secondary analysis of a randomized trial of methadone versus buprenorphine/naloxone.

Methods: 1241 individuals with OUD participated in an open label, pragmatic randomized trial of methadone versus buprenorphine/naloxone in nine treatment programs licensed to dispense agonist medication for OUD between 2006 to 2009. Read More

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http://dx.doi.org/10.1097/ADM.0000000000000630DOI Listing
February 2020

In-vitro investigation of calcitonin associated effects on the trophoblastic cells.

Acta Histochem 2020 Apr 3;122(3):151510. Epub 2020 Feb 3.

Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Histology and Embryology Department, Turkey. Electronic address:

Calcitonin is expressed in the epithelium of endometrium, and modulates zonula adherens junctions which are composed of cadherin-catenins complex during the implantation window. Trophoblastic cells which have complex interaction with the epithelial cells of endometrium during implantation were demonstrated to have calcitonin receptors. Mechanism of action of calcitonin on trophoblastic cells has not yet been elucidated. Read More

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http://dx.doi.org/10.1016/j.acthis.2020.151510DOI Listing

Monoubiquitination of p120-catenin is essential for TGFβ-induced epithelial-mesenchymal transition and tumor metastasis.

Sci Adv 2020 01 22;6(4):eaay9819. Epub 2020 Jan 22.

State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Biology, School of Life Sciences, Xiamen University, Fujian 361102, China.

Disassembly of intercellular junctions is a hallmark of epithelial-mesenchymal transition (EMT). However, how the junctions disassemble remains largely unknown. Here, we report that E3 ubiquitin ligase Smurf1 targets p120-catenin, a core component of adherens junction (AJ) complex, for monoubiquitination during transforming growth factor β (TGFβ)-induced EMT, thereby leading to AJ dissociation. Read More

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http://dx.doi.org/10.1126/sciadv.aay9819DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6976293PMC
January 2020

Desmosomal Junctions Govern Tissue Integrity and Actomyosin Contractility in Apoptotic Cell Extrusion.

Curr Biol 2020 Feb 30;30(4):682-690.e5. Epub 2020 Jan 30.

Mechanobiology Institute, Singapore, Level 5, T-lab Building, 5A Engineering Drive 1, Singapore 117411, Singapore; Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore. Electronic address:

During apoptosis, or programmed cell death, a dead cell could be expelled from the tissue by coordinated processes between the dying cell and its neighbors. Apoptotic cell extrusion is driven by actomyosin cable formation and its contraction and lamellipodial crawling of the neighboring cells [1-4]. Throughout cell extrusion, the mechanical coupling of epithelia needs to be maintained in order to preserve tissue homeostasis [1]. Read More

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http://dx.doi.org/10.1016/j.cub.2020.01.002DOI Listing
February 2020

Transition to a More even Distribution of Daily Protein intake Is Associated with Enhanced Fat Loss during a Hypocaloric and Physical Activity Intervention in Obese Older Adults.

J Nutr Health Aging 2020 ;24(2):210-217

Anne B. Newman, 5126 Public Health, 130 DeSoto Street, Pittsburgh, PA 15261, Office (412) 624-3056 Fax (412) 624-3737, Email:

Background: Optimization of intentional weight loss in obese older adults, through preferential fat mass reduction, is challenging, as the concomitant lean mass loss may exacerbate sarcopenia. Recent studies have suggested within-day distribution of protein intake plays a role in determining body composition remodeling. Here, we assessed whether changes in within-day protein intake distribution are related to improvements in body composition in overweight/obese older adults during a hypocaloric and exercise intervention. Read More

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http://dx.doi.org/10.1007/s12603-020-1313-8DOI Listing
January 2020

An optochemical tool for light-induced dissociation of adherens junctions to control mechanical coupling between cells.

Nat Commun 2020 01 24;11(1):472. Epub 2020 Jan 24.

Department of Cellular Biophysics, Max Planck Institute for Medical Research, Jahnstraße 29, D-69120, Heidelberg, Germany.

The cadherin-catenin complex at adherens junctions (AJs) is essential for the formation of cell-cell adhesion and epithelium integrity; however, studying the dynamic regulation of AJs at high spatio-temporal resolution remains challenging. Here we present an optochemical tool which allows reconstitution of AJs by chemical dimerization of the force bearing structures and their precise light-induced dissociation. For the dimerization, we reconstitute acto-myosin connection of a tailless E-cadherin by two ways: direct recruitment of α-catenin, and linking its cytosolic tail to the transmembrane domain. Read More

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http://dx.doi.org/10.1038/s41467-020-14390-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981158PMC
January 2020

Super-Resolution Imaging of Tight and Adherens Junctions: Challenges and Open Questions.

Int J Mol Sci 2020 Jan 23;21(3). Epub 2020 Jan 23.

Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), 13125 Berlin, Germany.

The tight junction (TJ) and the adherens junction (AJ) bridge the paracellular cleft of epithelial and endothelial cells. In addition to their role as protective barriers against bacteria and their toxins they maintain ion homeostasis, cell polarity, and mechano-sensing. Their functional loss leads to pathological changes such as tissue inflammation, ion imbalance, and cancer. Read More

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http://dx.doi.org/10.3390/ijms21030744DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037929PMC
January 2020

Acceptability and Feasibility of a Mobile Health Application for Video Directly Observed Therapy of Buprenorphine for Opioid Use Disorders in an Office-based Setting.

J Addict Med 2020 Jan 14. Epub 2020 Jan 14.

Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA (MEG); Division of General Internal Medicine, Department of Medicine, University of Washington, WA (JWK, JOM, KLB, JIT); Center of Excellence in Substance Abuse Treatment and Education, VA Puget Sound Health Care System, Seattle, WA (AJS); Department of Psychiatry and Behavioral Sciences, University of Washington, Seattle, WA (AJS); Section of General Internal Medicine, Department of Medicine, Boston Medical Center, Boston University School of Medicine, Boston, MA (JHS).

Introduction/background: Video directly observed therapy (video-DOT) through a mobile health platform may improve buprenorphine adherence and decrease diversion. This pilot study tested the acceptability and feasibility of using this technology among patients receiving buprenorphine in an office-based setting.

Methods: Participants were instructed to record videos of themselves taking buprenorphine. Read More

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http://dx.doi.org/10.1097/ADM.0000000000000608DOI Listing
January 2020

Traction force microscopy for understanding cellular mechanotransduction.

BMB Rep 2020 Feb;53(2):74-81

Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Cheonan 31151; Department of Integrated Biomedical Science, Soonchunhyang University, Asan 31538, Korea.

Under physiological and pathological conditions, mechanical forces generated from cells themselves or transmitted from extracellular matrix (ECM) through focal adhesions (FAs) and adherens junctions (AJs) are known to play a significant role in regulating various cell behaviors. Substantial progresses have been made in the field of mechanobiology towards novel methods to understand how cells are able to sense and adapt to these mechanical forces over the years. To address these issues, this review will discuss recent advancements of traction force microscopy (TFM), intracellular force microscopy (IFM), and monolayer stress microscopy (MSM) to measure multiple aspects of cellular forces exerted by cells at cell-ECM and cell-cell junctional intracellular interfaces. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061206PMC
February 2020
2.595 Impact Factor

Lessons learned from expanded reproductive carrier screening in self-reported Ashkenazi, Sephardi, and Mizrahi Jewish patients.

Mol Genet Genomic Med 2020 Feb 27;8(2):e1053. Epub 2019 Dec 27.

Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

Background: Next-generation sequencing (NGS)-based panels have gained traction as a strategy for reproductive carrier screening. Their value for screening Ashkenazi Jewish (AJ) individuals, who have benefited greatly from population-wide targeted testing, as well as Sephardi/Mizrahi Jewish (SMJ) individuals (an underserved population), has not been fully explored.

Methods: The clinical utilization by 6,805 self-reported Jewish individuals of an expanded NGS panel, along with several ancillary assays, was assessed retrospectively. Read More

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http://dx.doi.org/10.1002/mgg3.1053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005669PMC
February 2020

Inhibition of Protein Prenylation of GTPases Alters Endothelial Barrier Function.

Int J Mol Sci 2019 Dec 18;21(1). Epub 2019 Dec 18.

Department of Cardiology/Angiology, University Hospital Giessen, 35392 Giessen, Germany.

The members of Rho family of GTPases, RhoA and Rac1 regulate endothelial cytoskeleton dynamics and hence barrier integrity. The spatial activities of these GTPases are regulated by post-translational prenylation. In the present study, we investigated the effect of prenylation inhibition on the endothelial cytoskeleton and barrier properties. Read More

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http://dx.doi.org/10.3390/ijms21010002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981884PMC
December 2019

The tricellular vertex-specific adhesion molecule Sidekick facilitates polarised cell intercalation during Drosophila axis extension.

PLoS Biol 2019 12 5;17(12):e3000522. Epub 2019 Dec 5.

Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom.

In epithelia, tricellular vertices are emerging as important sites for the regulation of epithelial integrity and function. Compared to bicellular contacts, however, much less is known. In particular, resident proteins at tricellular vertices were identified only at occluding junctions, with none known at adherens junctions (AJs). Read More

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http://dx.doi.org/10.1371/journal.pbio.3000522DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6894751PMC
December 2019

β-catenin activation down-regulates cell-cell junction-related genes and induces epithelial-to-mesenchymal transition in colorectal cancers.

Sci Rep 2019 12 5;9(1):18440. Epub 2019 Dec 5.

Brain Korea 21 PLUS Project for Medical Science, Seoul, Korea.

WNT signaling activation in colorectal cancers (CRCs) occurs through APC inactivation or β-catenin mutations. Both processes promote β-catenin nuclear accumulation, which up-regulates epithelial-to-mesenchymal transition (EMT). We investigated β-catenin localization, transcriptome, and phenotypic differences of HCT116 cells containing a wild-type (HCT116-WT) or mutant β-catenin allele (HCT116-MT), or parental cells with both WT and mutant alleles (HCT116-P). Read More

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http://dx.doi.org/10.1038/s41598-019-54890-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895046PMC
December 2019

Reciprocal Association between the Apical Junctional Complex and AMPK: A Promising Therapeutic Target for Epithelial/Endothelial Barrier Function?

Int J Mol Sci 2019 Nov 29;20(23). Epub 2019 Nov 29.

Laboratory of Biological Science, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0811, Japan.

Epithelial/endothelial cells adhere to each other via cell-cell junctions including tight junctions (TJs) and adherens junctions (AJs). TJs and AJs are spatiotemporally and functionally integrated, and are thus often collectively defined as apical junctional complexes (AJCs), regulating a number of spatiotemporal events including paracellular barrier, selective permeability, apicobasal cell polarity, mechano-sensing, intracellular signaling cascades, and epithelial morphogenesis. Over the past 15 years, it has been acknowledged that adenosine monophosphate (AMP)-activated protein kinase (AMPK), a well-known central regulator of energy metabolism, has a reciprocal association with AJCs. Read More

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http://dx.doi.org/10.3390/ijms20236012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928779PMC
November 2019

Epithelial-specific isoforms of protein 4.1R promote adherens junction assembly in maturing epithelia.

J Biol Chem 2020 Jan 27;295(1):191-211. Epub 2019 Nov 27.

Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.

Epithelial adherens junctions (AJs) and tight junctions (TJs) undergo disassembly and reassembly during morphogenesis and pathological states. The membrane-cytoskeleton interface plays a crucial role in junctional reorganization. Protein 4. Read More

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http://dx.doi.org/10.1074/jbc.RA119.009650DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952607PMC
January 2020

Effects of cigarette smoke on barrier function and tight junction proteins in the bronchial epithelium: protective role of cathelicidin LL-37.

Respir Res 2019 Nov 9;20(1):251. Epub 2019 Nov 9.

Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.

Background: Airway epithelial barrier function is maintained by the formation of tight junctions (TJs) and adherens junctions (AJs). Inhalation of cigarette smoke causes airway epithelial barrier dysfunction and may contribute to the pathogenesis of chronic lung diseases such as asthma and chronic obstructive pulmonary disease (COPD). We assessed the effects of cigarette smoke on barrier function and expression of multiple TJ and AJ proteins in the bronchial epithelium. Read More

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http://dx.doi.org/10.1186/s12931-019-1226-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6842552PMC
November 2019

Role of α-Catenin and its mechanosensing properties in regulating Hippo/YAP-dependent tissue growth.

PLoS Genet 2019 11 7;15(11):e1008454. Epub 2019 Nov 7.

Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada.

α-catenin is a key protein of adherens junctions (AJs) with mechanosensory properties. It also acts as a tumor suppressor that limits tissue growth. Here we analyzed the function of Drosophila α-Catenin (α-Cat) in growth regulation of the wing epithelium. Read More

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http://dx.doi.org/10.1371/journal.pgen.1008454DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6863567PMC
November 2019

Binding partner- and force-promoted changes in αE-catenin conformation probed by native cysteine labeling.

Sci Rep 2019 10 25;9(1):15375. Epub 2019 Oct 25.

Departments of Structural Biology and Molecular & Cellular Physiology, Stanford University School of Medicine, Stanford, CA, 94305, USA.

Adherens Junctions (AJs) are cell-cell adhesion complexes that sense and propagate mechanical forces by coupling cadherins to the actin cytoskeleton via β-catenin and the F-actin binding protein αE-catenin. When subjected to mechanical force, the cadherin•catenin complex can tightly link to F-actin through αE-catenin, and also recruits the F-actin-binding protein vinculin. In this study, labeling of native cysteines combined with mass spectrometry revealed conformational changes in αE-catenin upon binding to the E-cadherin•β-catenin complex, vinculin and F-actin. Read More

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http://dx.doi.org/10.1038/s41598-019-51816-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6814714PMC
October 2019

Impaired hypotensive effects of centrally acting oxytocin in SHR and WKY rats exposed to chronic mild stress.

Am J Physiol Regul Integr Comp Physiol 2020 01 23;318(1):R160-R172. Epub 2019 Oct 23.

Chair and Department of Experimental and Clinical Physiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, Warsaw, Poland.

The present study was designed to determine the role of centrally acting oxytocin (OT) in the regulation of blood pressure during chronic mild stress (CMS) in spontaneously hypertensive (SHR; = 36) and normotensive Wistar-Kyoto (WKY; = 38) rats. The rats were implanted with osmotic minipumps for intracerebroventricular infusions of 0.9% NaCl, OT, and oxytocin receptor antagonist (OTANT) and divided into two groups: SHR and WKY ) exposed to 4-wk CMS and ) not exposed to stress (controls). Read More

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http://dx.doi.org/10.1152/ajpregu.00050.2019DOI Listing
January 2020

Comprehensive Benchmarking and Integration of Tumor Microenvironment Cell Estimation Methods.

Cancer Res 2019 12 22;79(24):6238-6246. Epub 2019 Oct 22.

Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, United Kingdom.

Various computational approaches have been developed for estimating the relative abundance of different cell types in the tumor microenvironment (TME) using bulk tumor RNA data. However, a comprehensive comparison across diverse datasets that objectively evaluates the performance of these approaches has not been conducted. Here, we benchmarked seven widely used tools and gene sets and introduced Consensus, a method that integrates gene sets from all the other methods for relative TME cell estimation of 18 cell types. Read More

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http://dx.doi.org/10.1158/0008-5472.CAN-18-3560DOI Listing
December 2019

Changes in the expression and distribution of junction and polarity proteins in the porcine endometrium during early pregnancy period.

Theriogenology 2020 Jan 1;142:196-206. Epub 2019 Oct 1.

Division of Reproductive Biology, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10, 10-748, Olsztyn, Poland.

The maternal endometrium undergoes transformations during early pregnancy period to regulate the paracellular permeability across the epithelium and to enable adhesion between the trophoblast and endometrial epithelial cells. These transformations, under the influence of ovarian hormones, are associated with a partial loss in polarity of epithelial cell that is regulated by tight junctions (TJ), adherens junctions (AJ) and associated polarity protein complexes. This study examined the change in expression and distribution of proteins associated with TJs, AJs and apical partition defective (PAR) complex in porcine endometrium on Days 10, 13 and 16 of estrous cycle and pregnancy. Read More

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http://dx.doi.org/10.1016/j.theriogenology.2019.09.041DOI Listing
January 2020
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A prognostic score for overall survival in patients treated with abiraterone in the pre- and post-chemotherapy setting.

Oncotarget 2019 Aug 20;10(49):5082-5091. Epub 2019 Aug 20.

Department of Urology, Stanford Medical School, Stanford, CA, USA.

Therapy resistance remains a serious dilemma in metastatic castration-resistant prostate cancer (mCRPC) with primary or secondary resistance frequently occurring against any given therapy. Available prognostic models for Abiraterone Acetate (AA) are specifically designed for either pre- or post-chemotherapy settings and mostly based on trial datasets not necessarily reflecting real-life. A score of 0-2 (low-risk) is associated with an OS-probability of 80. Read More

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http://dx.doi.org/10.18632/oncotarget.27133DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6707939PMC
August 2019
2 Reads

Vinculin anchors contractile actin to the cardiomyocyte adherens junction.

Mol Biol Cell 2019 10 4;30(21):2639-2650. Epub 2019 Sep 4.

Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261.

The adherens junction (AJ) couples the actin cytoskeletons of neighboring cells to allow mechanical integration and tissue organization. The physiological demands of intercellular adhesion require that the AJ be responsive to dynamic changes in force while maintaining mechanical load. These demands are tested in the heart, where cardiomyocyte AJs must withstand repeated cycles of actomyosin-mediated contractile force. Read More

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http://dx.doi.org/10.1091/mbc.E19-04-0216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761764PMC
October 2019
4.466 Impact Factor

Race and socioeconomic effect on sarcopenia and sarcopenic obesity in the Louisiana Osteoporosis Study (LOS).

JCSM Clin Rep 2018 Jul-Dec;3(2)

Tulane University, 1440 Canal St., Suite 2001, New Orleans, LA 70112.

Background: Sarcopenia and sarcopenic obesity are emerging public health issues. True prevalence rates are unknown and estimates differ substantially between studies. No large-scale single study has compared prevalence rates between whites, blacks, Asians, and Hispanics, as we intend to do here. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6712972PMC

Aeromonas sobria serine protease decreases epithelial barrier function in T84 cells and accelerates bacterial translocation across the T84 monolayer in vitro.

PLoS One 2019 16;14(8):e0221344. Epub 2019 Aug 16.

Laboratory of Molecular Microbiological Science, Faculty of Pharmaceutical Sciences, Hiroshima International University, Hiroshima, Japan.

Aeromonas sobria is a pathogen causing food-borne illness. In immunocompromised patients and the elderly, A. sobria can leave the intestinal tract, and this opportunistically leads to severe extraintestinal diseases including sepsis, peritonitis, and meningitis. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0221344PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6697317PMC
April 2020
2 Reads

Photopiperazines A-D, Photosensitive Interconverting Diketopiperazines with Significant and Selective Activity against U87 Glioblastoma Cells, from a Rare, Marine-Derived Actinomycete of the Family Streptomycetaceae.

J Nat Prod 2019 08 1;82(8):2262-2267. Epub 2019 Aug 1.

Center for Marine Biotechnology and Biomedicine , Scripps Institution of Oceanography, University of California, San Diego , La Jolla , California 92093-0204 , United States.

Photopiperazines A-D (-), unsaturated diketopiperazine derivatives, were isolated from the culture broth of a rare, marine-derived actinomycete bacterium, strain AJS-327. This strain shows very poor 16S rRNA sequence similarity to other members of the actinomycete family Streptomycetaceae, indicating it is likely a new lineage within this group. The structures of the photopiperazines were defined by analysis of HR-ESI-TOF-MS spectra in conjunction with the interpretation of 1D and 2D NMR data. Read More

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http://dx.doi.org/10.1021/acs.jnatprod.9b00429DOI Listing
August 2019
1 Read

The Tricellular Junction Protein Sidekick Regulates Vertex Dynamics to Promote Bicellular Junction Extension.

Dev Cell 2019 08 25;50(3):327-338.e5. Epub 2019 Jul 25.

Laboratory for Histogenetic Dynamics, Graduate School of Life Sciences, Tohoku University, 6-3 Aramaki Aza-Aoba, Aoba-ku, Sendai 980-8578, Japan. Electronic address:

Remodeling of cell-cell junctions drives cell intercalation that causes tissue movement during morphogenesis through the shortening and growth of bicellular junctions. The growth of new junctions is essential for continuing and then completing cellular dynamics and tissue shape sculpting; however, the mechanism underlying junction growth remains obscure. We investigated Drosophila genitalia rotation where continuous cell intercalation occurs to show that myosin II accumulating at the vertices of a new junction is required for the junction growth. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.06.017DOI Listing

Standard-flow LC and thermal focusing ESI elucidates altered liver proteins in late stage Niemann-Pick, type C1 disease.

Bioanalysis 2019 Jun;11(11):1067-1083

Department of Chemistry, University of Illinois at Chicago, Chicago, IL 60607, USA.

Mass spectrometry (MS)-based proteomics, particularly with the development of nano-ESI, have been invaluable to our understanding of altered proteins related to human disease. Niemann-Pick, type C1 (NPC1) disease is a fatal, autosomal recessive, neurodegenerative disorder. The resulting defects include unesterified cholesterol and sphingolipids accumulation in the late endosomal/lysosomal system resulting in organ dysfunction including liver disease. Read More

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https://www.future-science.com/doi/10.4155/bio-2018-0232
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http://dx.doi.org/10.4155/bio-2018-0232DOI Listing
June 2019
5 Reads

Clinical relevance of paliperidone palmitate 3-monthly in treating schizophrenia.

Neuropsychiatr Dis Treat 2019 21;15:1365-1379. Epub 2019 May 21.

The Zucker Hillside Hospital, Psychiatry Research, Glen Oaks, NY, USA.

Antipsychotics are the mainstay in schizophrenia management, and long-acting injectable (LAI) antipsychotics contribute to the successful maintenance of treatment by improving non-adherence and preventing relapses. Paliperidone palmitate 3-monthly (PP3M) formulation is the only available LAI antipsychotic that offers an extended 3-month window of stable plasma drug concentration, enabling only four injections per year. This paper summarizes clinically relevant endpoints from available evidence for PP3M to bridge translational research gaps and provide measurable outcomes that can be interpreted in clinical practice. Read More

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http://dx.doi.org/10.2147/NDT.S197225DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6535080PMC
May 2019
18 Reads

Endothelial cell Piezo1 mediates pressure-induced lung vascular hyperpermeability via disruption of adherens junctions.

Proc Natl Acad Sci U S A 2019 06 11;116(26):12980-12985. Epub 2019 Jun 11.

Department of Pharmacology, University of Illinois College of Medicine, University of Illinois at Chicago, Chicago, IL 60612;

Increased pulmonary microvessel pressure experienced in left heart failure, head trauma, or high altitude can lead to endothelial barrier disruption referred to as capillary "stress failure" that causes leakage of protein-rich plasma and pulmonary edema. However, little is known about vascular endothelial sensing and transduction of mechanical stimuli inducing endothelial barrier disruption. Piezo1, a mechanosensing ion channel expressed in endothelial cells (ECs), is activated by elevated pressure and other mechanical stimuli. Read More

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http://www.pnas.org/lookup/doi/10.1073/pnas.1902165116
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http://dx.doi.org/10.1073/pnas.1902165116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600969PMC
June 2019
12 Reads

Time-Variant SRC Kinase Activation Determines Endothelial Permeability Response.

Cell Chem Biol 2019 08 23;26(8):1081-1094.e6. Epub 2019 May 23.

Department of Pharmacology, The University of Illinois College of Medicine, 835 S. Wolcott Avenue, Chicago, IL 60612, USA. Electronic address:

In the current model of endothelial barrier regulation, the tyrosine kinase SRC is purported to induce disassembly of endothelial adherens junctions (AJs) via phosphorylation of VE cadherin, and thereby increase junctional permeability. Here, using a chemical biology approach to temporally control SRC activation, we show that SRC exerts distinct time-variant effects on the endothelial barrier. We discovered that the immediate effect of SRC activation was to transiently enhance endothelial barrier function as the result of accumulation of VE cadherin at AJs and formation of morphologically distinct reticular AJs. Read More

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http://dx.doi.org/10.1016/j.chembiol.2019.04.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6697609PMC
August 2019
3 Reads

N3ICD with the transmembrane domain can effectively inhibit EMT by correcting the position of tight/adherens junctions.

Cell Adh Migr 2019 12;13(1):203-218

a The central laboratory , Cancer Hospital of Shantou University Medical College , Shantou , China.

EMT allows a polarized epithelium to lose epithelial integrity and acquire mesenchymal characteristics. Previously, we found that overexpression of the intracellular domain of Notch3 (N3ICD) can inhibit EMT in breast cancer cells. In this study, we aimed to elucidate the influence of N3ICD or N3ICD combined with the transmembrane domain (TD+N3ICD) on the expression and distribution of TJs/AJs and polar molecules. Read More

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http://dx.doi.org/10.1080/19336918.2019.1619958DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6550553PMC
December 2019
7 Reads

Anterior joint space narrowing in patients with temporomandibular disorder.

J Orofac Orthop 2019 May 4;80(3):116-127. Epub 2019 Apr 4.

Department of Orthodontics, Seoul National University Dental Hospital, 101 Daehak-ro, Jongno-Gu, 03080, Seoul, South Korea.

Purpose: The aim of this work is to investigate changes in the anterior joint space (AJS) and the relationship of such changes to clinical symptoms in chronic temporomandibular joint disorder (TMD) patients, and to compare symptoms between chronic and acute TMD patients.

Methods: Data from 100 TMD patients diagnosed based on the Diagnostic Criteria for Temporomandibular Disorders (55 women; mean age 34.34 ± 14. Read More

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http://dx.doi.org/10.1007/s00056-019-00172-yDOI Listing
May 2019
10 Reads

VE-PTP stabilizes VE-cadherin junctions and the endothelial barrier via a phosphatase-independent mechanism.

J Cell Biol 2019 05 4;218(5):1725-1742. Epub 2019 Apr 4.

Department of Pharmacology and the Center for Lung and Vascular Biology, The University of Illinois College of Medicine, Chicago, IL

Vascular endothelial (VE) protein tyrosine phosphatase (PTP) is an endothelial-specific phosphatase that stabilizes VE-cadherin junctions. Although studies have focused on the role of VE-PTP in dephosphorylating VE-cadherin in the activated endothelium, little is known of VE-PTP's role in the quiescent endothelial monolayer. Here, we used the photoconvertible fluorescent protein VE-cadherin-Dendra2 to monitor VE-cadherin dynamics at adherens junctions (AJs) in confluent endothelial monolayers. Read More

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http://dx.doi.org/10.1083/jcb.201807210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6504901PMC
May 2019
5 Reads

Management of acute upper gastrointestinal bleeding.

BMJ 2019 Mar 25;364:l536. Epub 2019 Mar 25.

Section of Digestive Diseases, Yale School of Medicine, New Haven, and VA Connecticut Healthcare System, West Haven, Connecticut, CT 06520, USA.

Upper gastrointestinal bleeding (UGIB) is a common medical emergency, with a reported mortality of 2-10%. Patients identified as being at very low risk of either needing an intervention or death can be managed as outpatients. For all other patients, intravenous fluids as needed for resuscitation and red cell transfusion at a hemoglobin threshold of 70-80 g/L are recommended. Read More

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http://www.bmj.com/lookup/doi/10.1136/bmj.l536
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http://dx.doi.org/10.1136/bmj.l536DOI Listing
March 2019
25 Reads

Role of adherens junctions and apical-basal polarity of neural stem/progenitor cells in the pathogenesis of neurodevelopmental disorders: a novel perspective on congenital Zika syndrome.

Transl Res 2019 08 5;210:57-79. Epub 2019 Mar 5.

Center for Interdisciplinary Studies on the Nervous System (CISNe), Universidad Austral de Chile, Valdivia Chile; Centro de Investigación Biomédica (CIB), Facultad de Medicina, Universidad de los Andes, Santiago, Chile. Electronic address:

Radial glial cells (RGCs) are the neural stem/progenitor cells (NSPCs) that give rise to most of neurons and glial cells that constitute the adult central nervous system. A hallmark of RGCs is their polarization along the apical-basal axis. They extend a long basal process that contacts the pial surface and a short apical process to the ventricular surface. Read More

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http://dx.doi.org/10.1016/j.trsl.2019.02.014DOI Listing
August 2019
10 Reads