4,156 results match your criteria 8-ohdg levels

Effects of Gundelia tournefortii L. on biochemical parameters, antioxidant activities and DNA damage in a rat model of experimental obesity.

Braz J Biol 2021 20;83:e251198. Epub 2021 Sep 20.

Hakkari University, Institute of Sciences, Medical Services and Techniques, Hakkari, Turkey.

The present study was designed to investigate the effects of Gundelia tournefortii L. plant extract on different tissues in terms of DNA damage, biochemical and antioxidant parameter values in rats with high-calorie diets. With this aim, Wistar albino male rats were divided into 4 groups containing 6 rats each and the study was completed over 12 weeks duration. Read More

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September 2021

Urinary and Plasma Antioxidants in Behavioral Symptoms of Individuals With Autism Spectrum Disorder.

Front Psychiatry 2021 3;12:684445. Epub 2021 Sep 3.

Department of Pediatrics, Dokkyo Medical University, Mibu, Japan.

The balance between antioxidant capacity and oxidative stress-induced free radicals may be crucial in the pathophysiological development factor of autism spectrum disorder (ASD). We measured the following urinary and plasma biomarker levels of oxidative stress and antioxidants. As urinary biomarkers, (1) hexanoyl-lysine (HEL), which is a new biomarker of oxidative stress, (2) the total antioxidant capacity (TAC), and (3) 8-hydroxy-2'-deoxyguanosine (8-OHdG), as a product of oxidative modifications to DNA; and the plasma levels of (4) the antioxidant protein superoxide dismutase (SOD), which is the crucial defense again oxygen reactive species, and (5) transferrin and (6) ceruloplasmin, which are biomarkers of iron and copper neurotransmission and oxidant-antioxidant systems. Read More

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September 2021

Homalium zeylanicum attenuates streptozotocin-induced hyperglycemia and cellular stress in experimental rats via attenuation of oxidative stress imparts inflammation.

J Ethnopharmacol 2021 Sep 15:114649. Epub 2021 Sep 15.

Regional Plant Resource Centre, Medicinal & Aromatic Plant Division, Forest & Environment Department, Govt. of Odisha, Nayapalli, Bhubaneswar, 751015, India. Electronic address:

Ethnopharmacological Relevance: Homalium zeylanicum (Gardner) Benth (Salicaceae) leaves are being used as folklore medicine to treat diabetes by the local folk of Andhra Pradesh, India. The medicinal claim of this plant with hypoglycaemic effects was initially studied by the authors. Results demonstrated the important antioxidant activities of the hydroalcohol fraction of leaves of H. Read More

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September 2021

The modulatory role of prime identified compounds in Geophila repens in mitigating scopolamine-induced neurotoxicity in experimental rats of Alzheimer's disease via attenuation of cholinesterase, β-secretase, MAPt levels and inhibition of oxidative stress imparts inflammation.

J Ethnopharmacol 2021 Sep 14;282:114637. Epub 2021 Sep 14.

Regional Plant Resource Centre, Medicinal & Aromatic Plant Division, Forest & Environment Department, Govt. of Odisha, Nayapalli, Bhubaneswar, 751015, India. Electronic address:

Ethnopharmacological Relevance: Geophila repens (L.) I.M. Read More

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September 2021

Ameliorative effect of hesperidin on streptozotocin-diabetes mellitus-induced testicular DNA damage and sperm quality degradation in Sprague-Dawley rats.

J Food Biochem 2021 Sep 16:e13938. Epub 2021 Sep 16.

Division of Biochemistry, Faculty of Veterinary, Atatürk University, Erzurum, Turkey.

This study aimed to investigate the effect of hesperidin on reproductive damage caused by diabetes mellitus. A total of 24 adult male rats were divided into four groups: control group, hesperidin group, diabetes mellitus group, and diabetes mellitus + hesperidin group. The study was conducted for 4 weeks. Read More

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September 2021

Aconitine induces autophagy via activating oxidative DNA damage-mediated AMPK/ULK1 signaling pathway in H9c2 cells.

J Ethnopharmacol 2021 Sep 11;282:114631. Epub 2021 Sep 11.

School of Pharmacy and School of Public Health, Chengdu University of Traditional Chinese Medicine, Chengdu City, Sichuan Province, 611137, China; R&D Center for Efficiency, Safety and Application in Chinese Materia Medica with Medical and Edible Values, Chengdu University of Traditional Chinese Medicine, Chengdu City, Sichuan Province, 611137, China. Electronic address:

Ethnopharmacological Relevance: Aconitum species, with a medicinal history of 2000 years, was traditionally used in the treatment of rheumatism, arthritis, bruises, and pains. However, many studies have reported that Aconitum species can cause arrhythmia in experimental animals, resulting in myocardial fibrosis and cardiomyocyte damage. Cardiotoxicity is the main toxic effect of aconitine, but the detailed mechanism remains unclear. Read More

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September 2021

Metformin pretreatment reduces effect to dacarbazine and suppresses melanoma cell resistance.

Cell Biol Int 2021 Sep 10. Epub 2021 Sep 10.

Department of Pathological Sciences, Laboratory of Molecular Pathology, Londrina State University, UEL, Londrina, Parana, Brazil.

Oxidative stress role on metformin process of dacarbazine (DTIC) inducing resistance of B16F10 melanoma murine cells are investigated. To induce resistance to DTIC, murine melanoma cells were exposed to increasing concentrations of dacarabazine (DTIC-res group). Metformin was administered before and during the induction of resistance to DTIC (MET-DTIC). Read More

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September 2021

Prenatal Molecular Hydrogen Administration Ameliorates Several Findings in Nitrofen-Induced Congenital Diaphragmatic Hernia.

Int J Mol Sci 2021 Aug 31;22(17). Epub 2021 Aug 31.

Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-Cho, Showa-Ku, Nagoya 466-8550, Japan.

Oxidative stress plays a pathological role in pulmonary hypoplasia and pulmonary hypertension in congenital diaphragmatic hernia (CDH). This study investigated the effect of molecular hydrogen (H), an antioxidant, on CDH pathology induced by nitrofen. Sprague-Dawley rats were divided into three groups: control, CDH, and CDH + hydrogen-rich water (HW). Read More

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In vivo neurotoxic effects of emamectin benzoate in male mice: evaluation with enzymatic and biomolecular multi-biomarkers.

Özge Temiz

Environ Sci Pollut Res Int 2021 Sep 8. Epub 2021 Sep 8.

Vocational School of Health Services, Osmaniye Korkut Ata University, 80000, Osmaniye, Turkey.

The study of the toxic effects of emamectin benzoate (EMB) was conducted in male mice. Mice were randomly divided into 4 groups; control group, EMB25 group (1/30 LD = 25 mg/kg/day), EMB50 group (1/15 LD = 50 mg/kg/day), and EMB100 group (1/7.5 LD = 100 mg/kg/day). Read More

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September 2021

Influences of periodontitis on hippocampal inflammation, oxidative stress, and apoptosis in rats.

J Periodontal Res 2021 Sep 6. Epub 2021 Sep 6.

3D Medical and Industrial Design Laboratory, Istanbul University, Istanbul, Turkey.

Background And Aim: The hippocampus, which has a central role in cognitive and behavioral activities, is one of the most sensitive parts of the brain to systemic inflammatory diseases. This animal study aims to comprehensively investigate the possible inflammatory, oxidative, and apoptotic effects of periodontitis on the hippocampus.

Methods: Sixteen male Sprague-Dawley rats were randomly assigned to two groups: control and experimental periodontitis (Ep). Read More

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September 2021

Hydrogen-Rich Water Alleviates the Nickel-Induced Toxic Responses (Inflammatory Responses, Oxidative Stress, DNA Damage) and Ameliorates Cocoon Production in Earthworm.

Biol Trace Elem Res 2021 Sep 5. Epub 2021 Sep 5.

Research Center for Redox Applications in Foods (RCRAF), Igdir University, 76000 , Igdir, Turkey.

In recent years, studies investigating the protective effect of hydrogen-rich water (HRW) against different diseases and the toxicity of some substances have attracted increasing attention. Here, we assessed the effects of hydrogen-rich water on different nickel-induced toxic responses (reactive oxygen species (ROS), tumor necrosis factor-alpha (TNF-α), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) of stress responses, histopathological changes) and cocoon production in earthworm model. Earthworms were randomly divided into two main groups: water (W) group including control (CW: ultrapure water), 10 (10W), 200 (200W), and 500 (500W), and hydrogen-rich ultrapure water (HRW) group including control (CHRW: hydrogen-rich ultrapure water), 10 (10HRW), 200 (200HRW), and 500 (500HRW) mg of nickel chloride kg soil for 14 days. Read More

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September 2021

Prognostic Performance of Peripheral Blood Biomarkers in Identifying Seropositive Individuals at Risk of Developing Clinically Symptomatic Chagas Cardiomyopathy.

Microbiol Spectr 2021 Sep 25;9(1):e0036421. Epub 2021 Aug 25.

Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA.

Biomarkers for prognosis-based detection of Trypanosoma cruzi-infected patients presenting no clinical symptoms to cardiac Chagas disease (CD) are not available. In this study, we examined the performance of seven biomarkers in prognosis and risk of symptomatic CD development. T. Read More

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September 2021

Telmisartan Mitigates High-Glucose-Induced Injury in Renal Glomerular Endothelial Cells (rGECs) and Albuminuria in Diabetes Mice.

Chem Res Toxicol 2021 Sep 31;34(9):2079-2086. Epub 2021 Aug 31.

Department of Nephrology, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group, Huangshi, Hubei 435000, China.

Diabetic nephropathy (DN) is a common and severe complication of diabetes, impacting millions of people worldwide. High concentrations of serum glucose-associated injury of renal glomerular endothelial cells (rGECs) are involved in the DN pathogenesis. We found that exposure to high glucose increased the expression of angiotensin II type 1 receptor (AT1R) in human rGECs (hrGECs). Read More

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September 2021

Does puberty affect oxidative stress levels and antioxidant activity of saliva in patients with fixed orthodontic appliances?

J Orofac Orthop 2021 Aug 31. Epub 2021 Aug 31.

Faculty of Medicine, Department of Biochemistry, Suleyman Demirel University, Isparta, Turkey.

Purpose: To evaluate and compare patients' oxidative stress parameters and antioxidant status with fixed orthodontic appliances during the pubertal and postpubertal growth periods.

Methods: Saliva samples of 20 pubertal (mean age: 12.94 ± 0. Read More

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Artesunate Restrains Maturation of Dendritic Cells and Ameliorates Heart Transplantation-Induced Acute Rejection in Mice through the PERK/ATF4/CHOP Signaling Pathway.

Mediators Inflamm 2021 21;2021:2481907. Epub 2021 Aug 21.

Department of Cardiovascular Surgery, Renmin Hospital of Wuhan University, No. 238 Jiefang Road, Wuhan, 430060 Hubei, China.

Background: Heart transplantation (HT) is the only effective treatment for end-stage heart failure because it can effectively improve the survival rate and quality of life of patients with heart failure. Artesunate (ART) is an artemisinin derivative, with good water solubility and higher oral bioavailability. The main aim of this study was to determine the role of ART in HT mice. Read More

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The Effect of Iron Deficiency Anemia and Different Treatment Methods on DNA Damage: 8-hydroxy-2-deoxyguanosine Level.

Glob Pediatr Health 2021 25;8:2333794X211041337. Epub 2021 Aug 25.

Professor, Firat University Faculty of Medicine, Department of Biochemistry, Elazig, Turkey.

Ionic iron causes damages at the cellular level by forming free radicals. Reactive oxygen species lead to the formation of oxidative base damages in DNA. Among these forms the most common one and the one which has the best known mutagenity is 8-hydroxy-2'-deoxyguanosine (8-OHdG). Read More

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Mitochondrial dysfunction and oxidative stress in liver of male albino rats after exposing to sub-chronic intoxication of chlorpyrifos, cypermethrin, and imidacloprid.

Pestic Biochem Physiol 2021 Oct 20;178:104938. Epub 2021 Jul 20.

Department of Pesticide Chemistry and Technology, Laboratory of Bioenergetics and Membrane Toxicology, Faculty of Agriculture, 21545-El-Shatby, Alexandria University, Alexandria, Egypt.

The adverse effects of chlorpyrifos, cypermethrin, and imidacloprid on mitochondrial dysfunction and oxidative stress biomarkers were studied in rat liver. The liver deficiency was also confirmed by histological analysis and gel electrophoresis. Each insecticide was administered orally with five doses per week for 28 days to male albino rats at 1/50 of the LD per insecticide. Read More

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October 2021

Worsening of the Toxic Effects of (±)-4,4'-DMAR Following Its Co-Administration with (±)-4,4'-DMAR: Neuro-Behavioural, Physiological, Immunohistochemical and Metabolic Studies in Mice.

Int J Mol Sci 2021 Aug 16;22(16). Epub 2021 Aug 16.

LTTA Center and University Center of Gender Medicine, Department of Translational Medicine, Section of Legal Medicine, University of Ferrara, 44121 Ferrara, Italy.

4,4'-Dimethylaminorex (4,4'-DMAR) is a new synthetic stimulant, and only a little information has been made available so far regarding its pharmaco-toxicological effects. The aim of this study was to investigate the effects of the systemic administration of both the single (±) (0.1-60 mg/kg) and (±) (30 and 60 mg/kg) stereoisomers and their co-administration (e. Read More

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Efficacy and Safety of Sesame Oil Cake Extract on Memory Function Improvement: A 12-Week, Randomized, Double-Blind, Placebo-Controlled Pilot Study.

Nutrients 2021 Jul 28;13(8). Epub 2021 Jul 28.

Clinical Trial Center for Functional Foods, Jeonbuk National University Hospital, Jeonju 54907, Korea.

The goal of treatment for mild cognitive impairment (MCI) is to reduce the existing clinical symptoms, delay the progression of cognitive impairment and prevent the progression to Alzheimer's disease (AD). At present, there is no effective drug therapy for AD treatment. However, early intake of dietary supplements may be effective in alleviating and delaying the MCI. Read More

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Serum Inflammatory and Prooxidant Marker Levels in Different Periodontal Disease Stages.

Healthcare (Basel) 2021 Aug 20;9(8). Epub 2021 Aug 20.

CMT, Polytechnic University of Valencia, Camí de Vera, 46022 Valencia, Spain.

Background: Periodontitis has been associated to systemic diseases and this association could be due to an increase in circulating inflammatory and oxidative stress biomarkers in the periodontal disease. This study aimed to evaluate the relationship between inflammatory and pro-oxidant markers according to different stages of periodontitis.

Methods: This cross-sectional study included 70 subjects who were divided into three groups according to periodontitis stage: stage II (n = 22), stage III (n = 30), and stage IV (n = 18). Read More

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Association of Particulate Matter from Cooking Oil Fumes with Heart Rate Variability and Oxidative Stress.

Antioxidants (Basel) 2021 Aug 23;10(8). Epub 2021 Aug 23.

School of Public Health, National Defense Medical Center, Taipei 11490, Taiwan.

Many studies have reported various cardiovascular autonomic responses to ambient particulate matter (PM) pollution, but few have reported such responses to occupational PM exposures. Even fewer have demonstrated a relationship between PM pollution and oxidative stress in humans. This panel study evaluates the association between occupational exposure to PM in cooking oil fumes (COFs), and changes in both heart rate variability (HRV) and oxidative stress responses in 54 male Chinese cooks. Read More

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Urinary metabolites of multiple volatile organic compounds among general population in Wuhan, central China: Inter-day reproducibility, seasonal difference, and their associations with oxidative stress biomarkers.

Environ Pollut 2021 Aug 5;289:117913. Epub 2021 Aug 5.

Key Laboratory of Environment and Health (HUST), Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubation), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, PR China. Electronic address:

General population are concurrently and extensively exposed to many volatile organic compounds (VOCs), including some Group 1 human carcinogens, such as 1,3-butadiene. However, only a few studies assessed internal exposure levels of VOCs; particularly, very limited studies have examined associations between the urinary concentrations of multiple VOC metabolites (mVOCs) and oxidative stress biomarkers (OSBs) among the general population. In this study, 21 mVOCs and three OSBs including 8-hydroxy-2'-deoxyguanosine (8-OHdG; for DNA), 8-hydroxyguanosine (8-OHG; for RNA), and 4-hydroxy nonenal mercapturic acid (HNEMA; for lipid) were measured in 406 urine samples collected from 128 healthy adults during autumn and winter of 2018 in Wuhan, central China, including repeated samples taken in 3 d from 75 volunteers. Read More

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A Review of Oxidative Stress Products and Related Genes in Early Alzheimer's Disease.

J Alzheimers Dis 2021 Aug 17. Epub 2021 Aug 17.

Department of Applied Stem Cell Technologies, Technical Medical Centre, Faculty of Science and Technology, University of Twente, Enschede, The Netherlands.

Oxidative stress is associated with the progression of Alzheimer's disease (AD). Reactive oxygen species can modify lipids, DNA, RNA, and proteins in the brain. The products of their peroxidation and oxidation are readily detectable at incipient stages of disease. Read More

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CircTLK1 modulates sepsis-induced cardiomyocyte apoptosis via enhancing PARP1/HMGB1 axis-mediated mitochondrial DNA damage by sponging miR-17-5p.

J Cell Mol Med 2021 Sep 19;25(17):8244-8260. Epub 2021 Aug 19.

Department of Hepatopathy, Hunan Children's Hospital, Changsha, China.

Introduction: Septic cardiomyopathy is a common complication of sepsis with high morbidity and mortality, but lacks specific therapy. This study aimed to reveal the role of circTLK1 and its potential mechanisms in septic cardiomyopathy.

Materials And Methods: The in vitro and in vivo models of septic cardiomyopathy were established. Read More

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September 2021

Association of Tumour Microenvironment with Protein Glycooxidation, DNA Damage, and Nitrosative Stress in Colorectal Cancer.

Cancer Manag Res 2021 12;13:6329-6348. Epub 2021 Aug 12.

Department of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, Białystok, 15-222, Poland.

Purpose: In this study, we evaluated the total antioxidant capacity, nitrosative stress, and protein/DNA oxidation and glycoxidation products in patients with colorectal cancer regarding histopathological parameters associated with the tumour microenvironment, such as inflammatory infiltration and tumour budding and compare all determined parameters between tumours located in the right and left side of the colon and normal mucosa.

Patients And Methods: Ferric reducing antioxidant power (FRAP), nitrosative stress (myeloperoxidase (MPO), nitrogen oxide (NO), peroxynitrite, and nitrotyrosine), protein oxidation products (protein carbonyls (PC), total thiols, and ischemia modified albumin (IMA)), protein glycooxidation products (tryptophan, kynurenine, N-formylkynurenine, dityrosine, Amadori product, advanced glycation end products (AGE)) and 8-hydroxydeoxyguanosine (8-OHdG) were measured in homogenates from normal and cancerous tissue of 30 patients with colorectal cancer.

Results: Levels of FRAP (p=0. Read More

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Autophagy-mitophagy induction attenuates cardiovascular inflammation in a murine model of Kawasaki disease vasculitis.

JCI Insight 2021 Sep 22;6(18). Epub 2021 Sep 22.

Department of Pediatrics, Division of Infectious Diseases and Immunology.

Kawasaki disease (KD) is the leading cause of acquired heart disease among children. Murine and human data suggest that the NLRP3-IL-1β pathway is the main driver of KD pathophysiology. NLRP3 can be activated during defective autophagy/mitophagy. Read More

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September 2021

Exosomes Derived From Adipose-Derived Mesenchymal Stem Cells Ameliorate Radiation-Induced Brain Injury by Activating the SIRT1 Pathway.

Front Cell Dev Biol 2021 29;9:693782. Epub 2021 Jul 29.

Department of Burns and Cutaneous Surgery, Xijing Hospital, Air Force Military Medical University, Xi'an, China.

Objective: Studies have shown that the therapeutic effects of mesenchymal stem cells (MSCs) are mediated in a paracrine manner, mainly through extracellular vesicles such as exosomes. Here, we designed a study to investigate whether exosomes derived from adipose-derived mesenchymal stem cells (ADMSC-Exos) had protective effects in a rat model of radiation-induced brain injury and in microglia.

Methods: Male adult Sprague-Dawley (SD) rats were randomly divided into three groups: the control group, the radiation group (30 Gy), and the radiation + exosomes group (30 Gy + 100 ug exosomes). Read More

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Assessing oxidative stress resulting from environmental exposure to metals (Oids) in a middle Eastern population.

Environ Geochem Health 2021 Aug 14. Epub 2021 Aug 14.

Research Center for Health Sciences, Institute of Health, Department of Environmental Health Engineering, School of Public Health, Shiraz University of Medical Sciences, Razi blvd, Kuye Zahra Street, Shiraz, Iran.

Concentrations of metals and metalloids derived mainly from anthropogenic activities have increased considerably in the environment. Metals might be associated with increase reactive oxygen species (ROS) damage, potentially related to several health outcomes. This study has recruited 200 adult participants, including 110 males and 90 females in Shiraz (Iran), to investigate the relationship between chronic exposure to metals and ROS damage by analyzing malondialdehyde (MDA) and 8-Oxo-2'-deoxyguanosine (8-OHdG) concentrations, and has evaluated the associations between chronic metal exposure and ROS damage using regression analysis. Read More

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Dehydroepiandrosterone modulates oxidative DNA damage in pancreatic cancer: A case-control study.

JGH Open 2021 Aug 26;5(8):902-906. Epub 2021 Jun 26.

Department of Biology, Marvdasht Branch Islamic Azad University Marvdasht Iran.

Background And Aim: Dehydroepiandrosterone (DHEA) has a protective role against several types of cancer, although its mechanisms of action are still unknown, it may be related to the antioxidant effect of DHEA. We hypothesized that DHEA has a preventive effect on the formation of the 8-hydroxy-2'-deoxyguanosine (8-OHdG) DNA adduct in pancreatic cancer patients.

Methods: Serum DHEAs were quantified by the ELISA method in 50 pancreatic cancer patients with histopathological diagnosis of adenocarcinoma and 50 matched controls. Read More

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Involvement of endoplasmic reticulum stress in rotenone-induced leber hereditary optic neuropathy model and the discovery of new therapeutic agents.

J Pharmacol Sci 2021 Oct 22;147(2):200-207. Epub 2021 Jul 22.

Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, Gifu, Japan. Electronic address:

Leber hereditary optic neuropathy (LHON) is caused by mitochondrial DNA mutations and is the most common inherited mitochondrial disease. It is responsible for central vision loss in young adulthood. However, the precise mechanisms of onset are unknown. Read More

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October 2021