2,845 results match your criteria 599 peptide

[Influencing factors of weaning outcome of intensive care unit patients with planned extubation].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2021 May;33(5):563-567

Department of Intensive Care Unit, First Hospital of Qinhuangdao, Qinhuangdao 066000, Hebei, China. Corresponding author: Chen Xiaomin, Email:

Objective: To determine the risk factors of extubation failure and its effect on the prognosis of patients who had successfully passed a spontaneous breathing trial (SBT).

Methods: The clinical data of patients with mechanical ventilation more than 24 hours who passed SBT admitted to department of intensive care unit (ICU) of First Hospital of Qinhuangdao from November 2018 to November 2019 were retrospectively analyzed. According to the outcome of weaning within 48 hours after weaning, patients were divided into weaning success group and weaning failure group. Read More

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Label-free tracking of whole-cell response on RGD functionalized surfaces to varied flow velocities generated by fluidic rotation.

J Colloid Interface Sci 2021 Oct 20;599:620-630. Epub 2021 Apr 20.

Nanobiosensorics Laboratory, ELKH EK MFA, Budapest, Hungary. Electronic address:

Fluidic flow plays important roles in colloid and interface sciences. Measuring adsorption, aggregation processes and living cell behavior under a fluidic environment with varied flow velocities in a parallel and high-throughput manner remains to be a challenging task. Here a method is introduced to monitor cell response to well-defined flow with varied velocities over an array of label-free resonant waveguide grating (RWG) based optical biosensors. Read More

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October 2021

Co-assembled gold [email protected] core-shell nanospheres for aqueous Hg removal.

J Colloid Interface Sci 2021 Oct 21;599:436-442. Epub 2021 Apr 21.

Key Laboratory of Colloid and Interface Chemistry & Key Laboratory of Special Aggregated Materials (Shandong University), Ministry of Education, Jinan 250100, China. Electronic address:

The construction of peptide and metal nanoparticles hybrid is attractive to explore their synergistic properties and applications extensively. However, it remains a challenge to fabricate a well-defined and size-controllable short peptide/Au nanoparticles hybrid. Here, we report a facile and flexible co-assembly strategy for the construction of tripeptide coated Au nanorods (AuNRs). Read More

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October 2021

Mass Spectral Analysis of Synthetic Peptides: Implications in Proteomics.

J Biomol Tech 2021 04;32(1):30-35

CSIR-Centre for Cellular and Molecular Biology, Hyderabad 500007, India.

Sequence determination of peptides is a crucial step in mass spectrometry-based proteomics. Peptide sequences are determined either by database search or by sequencing using tandem mass spectrometry. Determination of all the theoretical expected peptide fragments and eliminating false discoveries remains a challenge in proteomics. Read More

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Identification of an allosteric and Smad3-selective inhibitor of p38αMAPK using a substrate-based approach.

Bioorg Med Chem Lett 2021 Jul 21;43:128056. Epub 2021 Apr 21.

Graduate School of Science, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai-shi, Osaka 599-8531, Japan.

p38α mitogen activated protein kinase (MAPK) plays important roles in multiple cellular functions by phosphorylating a wide variety of substrates, and therefore, p38α MAPK has been considered as an important drug target. In this study, we designed peptide-based inhibitors for p38α MAPK, which can only inhibit the Smad3 phosphorylation specifically, by targeting the KIM binding site of p38α MAPK. Peptide 6 showed a significant inhibitory potency for the Smad3 phosphorylation by p38α MAPK. Read More

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Involvement of TRPC5 channels, inwardly rectifying K channels, PLCβ and PIP in vasopressin-mediated excitation of medial central amygdala neurons.

J Physiol 2021 Jun 27;599(12):3101-3119. Epub 2021 Apr 27.

Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND58203, USA.

Key Points: Activation of V vasopressin receptors facilitates neuronal excitability in the medial nucleus of central amygdala (CeM) V receptor activation excites about 80% CeM neurons by opening a cationic conductance and about 20% CeM neurons by suppressing an inwardly rectifying K (Kir) channel The cationic conductance activated by V receptors is identified as TRPC5 channels PLCβ-mediated depletion of PIP is involved in V receptor-elicited excitation of CeM neurons Intracellular Ca release and PKC are unnecessary for V receptor-mediated excitation of CeM neurons ABSTRACT: Arginine vasopressin (AVP) serves as a hormone in the periphery to modulate water homeostasis and a neuromodulator in the brain to regulate a diverse range of functions including anxiety, social behaviour, cognitive activities and nociception. The amygdala is an essential brain region involved in modulating defensive and appetitive behaviours, pain and alcohol use disorders. Whereas activation of V receptors in the medial nucleus of the central amygdala (CeM) increases neuronal excitability, the involved ionic and signalling mechanisms have not been determined. Read More

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Advancing peptide siRNA-carrier designs through L/D-amino acid stereochemical modifications to enhance gene silencing.

Mol Ther Nucleic Acids 2021 Jun 19;24:462-476. Epub 2021 Mar 19.

Department of Oral Health Sciences, James B. Edwards College of Dental Medicine, Medical University of South Carolina (MUSC), Charleston, SC 29425, USA.

The 599 peptide has been previously shown to effectively deliver small interfering RNAs (siRNAs) to cancer cells, inducing targeted-oncogene silencing, with a consequent inhibition of tumor growth. Although effective, this study was undertaken to advance the 599 peptide siRNA-carrier design through L/D-amino acid stereochemical modifications. Consequently, 599 was modified to generate eight different peptide variants, incorporating either different stereochemical patterns of L/D-amino acids or a specific D-amino acid substitution. Read More

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[Combined prognostic value of serum lactic acid, procalcitonin and severity score for short-term prognosis of septic shock patients].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2021 Mar;33(3):281-285

Department of Critical Care Medicine, Affiliated Hospital of Jining Medical University, Jining 272030, Shandong, China. Corresponding author: Hu Qinghe, Email:

Objective: To explore the value of lactic acid (Lac), procalcitonin (PCT), sequential organ failure assessment (SOFA) score and acute physiology and chronic health evaluation II (APACHE II) score in assessing the severity and predicting the prognosis in sepsis shock.

Methods: A retrospectively study was conducted. Patients with septic shock hospitalized in the department of critical care medicine of the Affiliated Hospital of Jining Medical University from April 2015 to June 2019 were enrrolled. Read More

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In-silico structural analysis of Pseudomonas syringae effector HopZ3 reveals ligand binding activity and virulence function.

J Plant Res 2021 May 17;134(3):599-611. Epub 2021 Mar 17.

, 50, Banerjee para road, Naihati, 743165, West Bengal, India.

Bacterial acetyltransferase effectors belonging to the Yersinia outer protein J (YopJ) group inhibit multiple immune signaling pathways in human and plants. The present study determines in-silico acetyl-coenzyme A (AcCoA) binding and Arabidopsis immune regulator RPM1-interacting protein4 (RIN4) peptide interactions to YopJ effector hypersensitivity and pathogenesis-dependent outer proteinZ3 (HopZ3) from Pseudomonas syringae. Phylogenetic analysis revealed that HopZ3 was clustered by acetyltransferase effectors from plant bacterial pathogens. Read More

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Internal cell-penetrating peptide-mediated internalization enables a chimeric lysin to target intracellular pathogens.

Int J Pharm 2021 Apr 9;599:120449. Epub 2021 Mar 9.

CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China; University of the Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Intracellular pathogens pose serious challenges to the public health worldwide. Lysin, peptidoglycan hydrolase from phage, is promising alternative to conventional antibiotics because of its high bactericidal activity and low risk of resistance. However, most proteinaceous lysins cannot penetrate the mammalian cell membrane because of size exclusion. Read More

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MiR-599 Protects Cardiomyocytes against Oxidative Stress-Induced Pyroptosis.

Biomed Res Int 2021 18;2021:3287053. Epub 2021 Feb 18.

Department of Cardiology, Heilongjiang Provincial People's Hospital, Harbin 150086, China.

Oxidative stress is a crucial factor and key promoter of a variety of cardiovascular diseases associated with cardiomyocyte injury. Emerging literatures suggest that pyroptosis plays a key role in cardiac damages. However, whether pyroptosis contributes to cardiomyocyte injury under oxidative stress and the underlying molecular mechanisms are totally unclear. Read More

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The APEX1/miRNA-27a-5p axis plays key roles in progression, metastasis and targeted chemotherapy of gastric cancer.

Int J Pharm 2021 Apr 4;599:120446. Epub 2021 Mar 4.

National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710119, China. Electronic address:

Gastric cancer (GC) presents a challenge for conventional therapeutics due to low targeting specificity and subsequent elicitation of multiple drug resistance (MDR). As an essential enzyme for DNA repair, apurinic/apyrimidinic endodeoxyribonuclease 1 (APEX1) exhibits multiple functions to affect cancer malignancy and is excessively expressed in GC. However, the roles APEX1 and its inhibitor miR-27a-5p play in modulating GC progression and MDR development remains unclear. Read More

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Collagen Type I Biomaterials as Scaffolds for Bone Tissue Engineering.

Polymers (Basel) 2021 Feb 17;13(4). Epub 2021 Feb 17.

Department of Cell Biology, Genetics and Physiology, Faculty of Science, University of Málaga, IBIMA, 29071 Málaga, Spain.

Collagen type I is the main organic constituent of the bone extracellular matrix and has been used for decades as scaffolding material in bone tissue engineering approaches when autografts are not feasible. Polymeric collagen can be easily isolated from various animal sources and can be processed in a great number of ways to manufacture biomaterials in the form of sponges, particles, or hydrogels, among others, for different applications. Despite its great biocompatibility and osteoconductivity, collagen type I also has some drawbacks, such as its high biodegradability, low mechanical strength, and lack of osteoinductive activity. Read More

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February 2021

[Cloning and expression analysis of protein kinase C gene from Whitmania pigra].

Zhongguo Zhong Yao Za Zhi 2021 Feb;46(3):599-604

Institute of Chinese Medicinal Materials, Nanjing Agricultural University Nanjing 210095, China.

Protein kinase C(PKC) is a type of protein kinase widely involved in cell proliferation and development, but the developmental mechanism in the gonads of androgynous animals is still unclear. In order to explore the role of protein kinase C in the development of Whitmania pigra germ cells, the Wh. pigra PKC(Wp-PKC) gene was cloned, bioinformatics analysis was conducted, and fluorescent quantitative PCR was used to analyze the expression of female and male gonads. Read More

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February 2021

Endosomal escape cell-penetrating peptides significantly enhance pharmacological effectiveness and CNS activity of systemically administered antisense oligonucleotides.

Int J Pharm 2021 Apr 26;599:120398. Epub 2021 Feb 26.

The Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville 3010, VIC, Australia. Electronic address:

Antisense oligonucleotides (ASOs) are an emerging class of gene-specific therapeutics for diseases associated with the central nervous system (CNS). However, ASO delivery across the blood-brain barrier (BBB) to their CNS target cells remains a major challenge. Since ASOs are mainly taken up into the brain capillary endothelial cells interface through endosomal routes, entrapment in the endosomal compartment is a major obstacle for efficient CNS delivery of ASOs. Read More

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Immobilisation induces sizeable and sustained reductions in forearm glucose uptake in just 24 h but does not change lipid uptake in healthy men.

J Physiol 2021 04 10;599(8):2197-2210. Epub 2021 Mar 10.

Centre for Sport, Exercise and Osteoarthritis Research Versus Arthritis, University of Nottingham, Nottingham, UK.

Key Points: The trajectory, magnitude and localisation of metabolic perturbations caused by immobilisation (IMM) are unresolved. Forearm glucose uptake (FGU) in response to glucose feeding was determined in healthy men before and during 72 h of forearm IMM, and the same measurements were made in the non-IMM contralateral limb at baseline and 72 h. In a similar study design, FGU and forearm lipid uptake were determined after a high fat mixed-meal (HFMM) in IMM and non-IMM limbs. Read More

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The Predictive Value of Interleukin-6 and Neutrophil-Lymphocyte Ratio in Patients with Severe and Extremely Severe Oral and Maxillofacial Space Infections.

Biomed Res Int 2021 28;2021:2615059. Epub 2021 Jan 28.

Oncology Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.

Objective: To investigate the correlation between clinical manifestation and neutrophil-lymphocyte ratio, C-reactive protein (CRP), and interleukin-6 (IL-6) in the patients with severe and extremely severe oral and maxillofacial space infection (OMSI).

Methods: In this retrospective study, we included 18 patients with severe and extremely severe OMSI from November 2012 to October 2018. Pearson or Spearman correlation coefficients were calculated to measure the association between the number of complications and locations and the number and percentage of lymphocyte, leukocyte, neutrophil, eosinophil, basophil, monocyte, CRP, and IL-6. Read More

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The anti-inflammatory cytokine response characterized by elevated interleukin-10 is a stronger predictor of severe disease and poor outcomes than the pro-inflammatory cytokine response in coronavirus disease 2019 (COVID-19).

Clin Chem Lab Med 2021 02 26;59(3):599-607. Epub 2020 Nov 26.

Department of Emergency Medicine, University of Cincinnati, Cincinnati, OH, USA.

Objectives: Severe coronavirus disease 2019 (COVID-19) is associated with a dysregulated immune state. While research has focused on the hyperinflammation, little research has been performed on the compensatory anti-inflammatory response. The aim of this study was to evaluate the anti-inflammatory cytokine response to COVID-19, by assessing interleukin-10 (IL-10) and IL-10/lymphocyte count ratio and their association with outcomes. Read More

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February 2021

Evolving new-age strategies to transport therapeutics across the blood-brain-barrier.

Int J Pharm 2021 Apr 2;599:120351. Epub 2021 Feb 2.

Department of Pharmacy, Birla Institute of Technology and Science, Pilani, Pilani Campus, Rajasthan 333031, India; R&D Healthcare Division Emami Ltd., 13, BT Road, Belgharia, Kolkata 700056, India. Electronic address:

A basic understanding of the blood-brain barrier (BBB) is essential for the novel advancements in targeting drugs specific to the brain. Neoplasm compromising the internal structure of BBB that results in impaired vasculature is called as blood tumor barrier (BTB). Besides, the BBB serves as a chief hindrance to the passage of a drug into the brain parenchyma. Read More

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The role of chaperone complex HSP90-SGT1-RAR1 as the associated machinery for hybrid inviability between Nicotiana gossei Domin and N. tabacum L.

Gene 2021 Apr 21;776:145443. Epub 2021 Jan 21.

Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan. Electronic address:

Two cultured cell lines (GTH4 and GTH4S) of a Nicotiana interspecific F hybrid (N. gossei × N. tabacum) were comparatively analyzed to find genetic factors related to hybrid inviability. Read More

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Synergistic interaction of nerve growth factor and glial cell-line derived neurotrophic factor in muscular mechanical hyperalgesia in rats.

J Physiol 2021 03 2;599(6):1783-1798. Epub 2021 Feb 2.

Department of Physical Therapy, College of Life Sciences, Chubu University, Kasugai, 487-8501, Japan.

Key Points: Nerve growth factor (NGF) and glial cell line-derived neurotrophic factor (GDNF) are essential for neuronal development and survival in embryo. However, after birth they play pivotal roles in the generation of hyperalgesia in many painful conditions. Both factors are believed to act on different groups of primary afferents, but interaction between them has not yet been studied. Read More

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Domain-wise differentiation of Mycobacterium tuberculosis H Rv hypothetical proteins: A roadmap to discover bacterial survival potentials.

Biotechnol Appl Biochem 2021 Jan 19. Epub 2021 Jan 19.

Centre for Interdisciplinary Research in Basic Science, Jamia Millia Islamia, Jamia Nagar, New Delhi, India.

Proteomic information revealed approximately 3,923 proteins in Mycobacterium tuberculosis H Rv genome of which around ∼25% of proteins are hypothetical proteins (HPs). The present work comprises computational approaches to identify and characterize the HPs of M. tuberculosis that symbolize the putative target for rationale development of a drug or antituberculosis strategy. Read More

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January 2021

Transcriptomic, proteomic, and biochemical analyses reveal a novel neuritogenesis mechanism of Naja naja venom α-elapitoxin post binding to TrkA receptor of rat pheochromocytoma cells.

J Neurochem 2020 12 2;155(6):612-637. Epub 2020 Oct 2.

Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, School of Sciences, Tezpur University, Tezpur, Assam, India.

This is the first report showing unique neuritogenesis potency of Indian Cobra N. naja venom long-chain α-neurotoxin (Nn-α-elapitoxin-1) exhibiting no sequence similarity to conventional nerve growth factor, by high-affinity binding to its tyrosine kinase A (TrkA) receptor of rat pheochromocytoma (PC-12) cells without requiring low-affinity receptor pNTR. The binding residues between Nn-α-elapitoxin-1 and mammalian TrkA receptor are predicted by in silico analysis. Read More

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December 2020

Assessing SIRAH's Capability to Simulate Intrinsically Disordered Proteins and Peptides.

J Chem Theory Comput 2021 Feb 7;17(2):599-604. Epub 2021 Jan 7.

Institut Pasteur de Montevideo, Mataojo 2020, Montevideo, CP 11400, Uruguay.

The challenges posed by intrinsically disordered proteins (IDPs) to atomistic and coarse-grained (CG) simulations are boosting efforts to develop and reparametrize current force fields. An assessment of the dynamical behavior of IDPs' and unstructured peptides with the CG SIRAH force field suggests that the current version achieves a fair description of IDPs' conformational flexibility. Moreover, we found a remarkable capability to capture the effect of point mutations in loosely structured peptides. Read More

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February 2021

Elevated levels of TNF-α, IL-1β and IL-6 in the synovial tissue of patients with labral tear: a comparative study with hip osteoarthritis.

BMC Musculoskelet Disord 2021 Jan 6;22(1):33. Epub 2021 Jan 6.

Department of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-ku, Kitasato, Sagamihara City, Kanagawa, 252-0374, Japan.

Background: Labral tear can be the initiating factor in the onset of hip osteoarthritis (HOA). However, the physiopathology of labral tear is not fully understood. Our aim was to compare synovial tissue inflammatory cytokine levels between patients with labral tear and late-stage HOA. Read More

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January 2021

Ligand-induced transition in conformations of vicinal cysteine disulfides in proteins.

Proteins 2021 Jun 25;89(6):599-613. Epub 2021 Mar 25.

Department of Chemistry, School of Chemical Sciences, Central University of Karnataka, Kalaburagi, India.

Vicinal cysteine disulfides are thought to be associated with specific conformations of cysteine disulfides due to the restricted rotation of single bonds in an eight-membered cyclic disulfide loop. Conformations of vicinal cysteine disulfides are analyzed using χ , χ , χ , χ ', χ ' torsion angles in the crystal structures of proteins retrieved from Protein Data Bank (PDB). 85% of vicinal disulfides have (+, -)LHStaple conformation with trans configuration of the peptide bond and 9% have (-, -)RHStaple conformation with cis configured peptide bond. Read More

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Abnormal cerebellar function and tremor in a mouse model for non-manifesting partially penetrant dystonia type 6.

J Physiol 2021 04 9;599(7):2037-2054. Epub 2021 Jan 9.

Department of Pathology & Immunology, Baylor College of Medicine, Houston, TX, USA.

Key Points: Loss-of-function mutations in the Thap1 gene cause partially penetrant dystonia type 6 (DYT6). Some non-manifesting DYT6 mutation carriers have tremor and abnormal cerebello-thalamo-cortical signalling. We show that Thap1 heterozygote mice have action tremor, a reduction in cerebellar neuron number, and abnormal electrophysiological signals in the remaining neurons. Read More

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Effects of miR-599 targeting YAP1 on proliferation, invasion and apoptosis of bladder urothelial carcinoma cells.

Exp Mol Pathol 2021 02 29;118:104599. Epub 2020 Dec 29.

Department of Urology, Shanghai Pudong Hew Histrict Zhoupu Hospital, Shanghai 201318, China.

The aim of this study was to investigate the effects of microRNA (miR)-599 targeting Yes-associated protein 1 (YAP1) on the proliferation, invasion and apoptosis of bladder urothelial carcinoma cell line J82. J82 cells cultured in vitro were divided into miR-nc group (transfected with analog negative control miR-NC), miR-599 group (transfected with miR-599 analog), miR-599 + pcDNA3.1 group (co transfected with miR-599 analog and pcDNA3. Read More

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February 2021

TSPAN-7 as a key regulator of glucose-stimulated Ca influx and insulin secretion.

Nordine Helassa

J Physiol 2021 03 30;599(6):1733-1734. Epub 2020 Dec 30.

Liverpool Centre for Cardiovascular Science, Department of Cardiovascular Science and Metabolic Medicine, Institute of Life Course and Medical Sciences, Faculty of Health and Life Sciences, University of Liverpool, Liverpool, L69 3BX, UK.

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No matter the type, when it comes to recovery all fibres are similar.

Andrew J Lovell

J Physiol 2021 03 28;599(6):1737-1738. Epub 2020 Dec 28.

University of Guelph, Ontario, Canada.

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