39 results match your criteria 3d-printed wells


Recapitulation of First Pass Metabolism Using 3D Printed Microfluidic Chip and Organoid.

Cells 2021 11 25;10(12). Epub 2021 Nov 25.

CHA Organoid Research Center, Department of Microbilogy, School of Medicine, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam 13488, Gyeonggi-do, Korea.

The low bioavailability of oral drugs due to first pass metabolism is a major obstacle in drug development. With significant developments in the field of in vitro organ modeling and microfluidic chip three-dimensional (3D) printing, the challenge is to apply these for the production and evaluation of new drug candidates. This study aimed to produce a microfluidic chip to recapitulate and assess the feasibility of the first pass metabolism. Read More

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November 2021

Dual-Material 3D-Printed Intestinal Model Devices with Integrated Villi-like Scaffolds.

ACS Appl Mater Interfaces 2021 Dec 6;13(49):58434-58446. Epub 2021 Dec 6.

Center for Intestinal Absorption and Transport of Biopharmaceuticals, Department of Health Technology, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.

small intestinal models aim to mimic the intestinal function and structure, including the villi architecture of the native tissue. Accurate models in a scalable format are in great demand to advance, for example, the development of orally administered pharmaceutical products. Widely used planar intestinal cell monolayers for compound screening applications fail to recapitulate the three-dimensional (3D) microstructural characteristics of the intestinal villi arrays. Read More

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December 2021

Comparison of Design Approaches for Low-Cost Sampling Mechanisms in Open-Source Chemical Instrumentation.

HardwareX 2021 Oct 10;10. Epub 2021 Aug 10.

Department of Chemistry & Biochemistry, Rowan University, Glassboro, NJ.

Robotic positioning systems are used in a variety of chemical instruments, primarily for liquid handling purposes, such as autosamplers from vials or well plates. Here, two approaches to the design of open-source autosampler positioning systems for use with 96-well plates are described and compared. The first system, a 3-axis design similar to many low-cost 3D printers that are available on the market, is constructed using an aluminum design and stepper motors. Read More

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October 2021

Tools to Image Germplasm Dynamics During Early Zebrafish Development.

Front Cell Dev Biol 2021 13;9:712503. Epub 2021 Aug 13.

Warwick Medical School, University of Warwick, Coventry, United Kingdom.

During the first day of zebrafish development, ribonucleoprotein (RNP) complexes called germplasm form large aggregates that initially segregate asymmetrically during cleavage stages. After zygotic genome activation, the granules break into smaller fragments that associate with the nuclear membrane as perinuclear (germ) granules toward the end of gastrulation. The mechanisms underlying the highly dynamic behavior of germ granules are not well studied but thought to be facilitated by the cytoskeleton. Read More

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A High Throughput Microplate Feeder Assay for Quantification of Consumption in Drosophila.

J Vis Exp 2021 06 14(172). Epub 2021 Jun 14.

Department of Genetics and Biochemistry and Center for Human Genetics, Clemson University;

Quantifying food intake in Drosophila is used to study the genetic and physiological underpinnings of consumption-associated traits, their environmental factors, and the toxicological and pharmacological effects of numerous substances. Few methods currently implemented are amenable to high throughput measurement. The Microplate Feeder Assay (MFA) was developed for quantifying the consumption of liquid food for individual flies using absorbance. Read More

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Breaking the Third Wall: Implementing 3D-Printing Technics to Expand the Complexity and Abilities of Multi-Organ-on-a-Chip Devices.

Micromachines (Basel) 2021 May 28;12(6). Epub 2021 May 28.

Membrane and Liposome Research Lab, Hebrew University Hadassah Medical School, Jerusalem 91120, Israel.

The understanding that systemic context and tissue crosstalk are essential keys for bridging the gap between in vitro models and in vivo conditions led to a growing effort in the last decade to develop advanced multi-organ-on-a-chip devices. However, many of the proposed devices have failed to implement the means to allow for conditions tailored to each organ individually, a crucial aspect in cell functionality. Here, we present two 3D-print-based fabrication methods for a generic multi-organ-on-a-chip device: One with a PDMS microfluidic core unit and one based on 3D-printed units. Read More

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Construct validation of a 3D printed neonatal thoracoscopic simulator: Can it measure expertise?

J Pediatr Surg 2021 Nov 4;56(11):1962-1965. Epub 2021 Apr 4.

Canterbury District Health Board, New Zealand; University of Otago, 2 Riccarton Ave, Christchurch Central, Christchurch 8011, New Zealand.

Background: acquiring technical expertise for neonatal thoracoscopy is challenging. To address this, we designed a fully synthetic thoracoscopic simulator for which we established its construct validity.

Methods: three thoracoscopic tasks were assessed: ring transfer, needle pass and incision of a blind upper esophageal pouch (EA cut). Read More

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November 2021

3D-Printing Replication of Porous Media for Lab-Scale Characterization Research.

ACS Omega 2021 Feb 15;6(4):2655-2664. Epub 2021 Jan 15.

University of North Dakota, Grand Forks, North Dakota 58202, United States.

Simplifying fluid-flow physics in conventional reservoirs is convenient by assuming uniform lithology and system-geometry with minimal rock/hydrocarbon interactions. Such simplification restrains mathematical models' ability to simulate unconventional reservoirs' actual flow behavior and production performance. Researchers can achieve precise adaption for the physics of fluid flow in porous media if they geometrically characterize the system under study appropriately, and there are minimal interactions indeed. Read More

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February 2021

96-Well Oxygen Control Using a 3D-Printed Device.

Anal Chem 2021 02 18;93(4):2570-2577. Epub 2021 Jan 18.

Department of Bioengineering, University of Illinois at Chicago, 851 South Morgan Street, Chicago, Illinois 60607, United States.

Oxygen concentration varies tremendously within the body and has proven to be a critical variable in cell differentiation, proliferation, and drug metabolism among many other physiological processes. Currently, researchers study the gas's role in biology using low-throughput gas control incubators or hypoxia chambers in which all cells in a vessel are exposed to a single oxygen concentration. Here, we introduce a device that can simultaneously deliver 12 unique oxygen concentrations to cells in a 96-well plate and seamlessly integrate into biomedical research workflows. Read More

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February 2021

3D-printed assistive pipetting system for gel electrophoresis for technicians with low acuity vision.

Biotechniques 2021 01 14;70(1):49-53. Epub 2020 Dec 14.

Department of Ophthalmology, University of Washington, Seattle, WA, USA.

In molecular biology laboratories, many tasks require fine motor control and high acuity vision. For example, lab technicians with visual impairment experience difficulty loading samples into the small wells of a horizontal agarose gel. We have developed a 3D-printable gel loading system which allows technicians with low-contrast vision to load gels correctly. Read More

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January 2021

Miniaturized free-flow electrophoresis: production, optimization, and application using 3D printing technology.

Electrophoresis 2021 02 22;42(3):305-314. Epub 2020 Nov 22.

Institute of Technical Chemistry, Leibniz University Hannover, Callinstraße 5, Hannover, 30167, Germany.

The increasing resolution of three-dimensional (3D) printing offers simplified access to, and development of, microfluidic devices with complex 3D structures. Therefore, this technology is increasingly used for rapid prototyping in laboratories and industry. Microfluidic free flow electrophoresis (μFFE) is a versatile tool to separate and concentrate different samples (such as DNA, proteins, and cells) to different outlets in a time range measured in mere tens of seconds and offers great potential for use in downstream processing, for example. Read More

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February 2021

A universal multi-platform 3D printed bioreactor chamber for tendon tissue engineering.

J Tissue Eng 2020 Jan-Dec;11:2041731420942462. Epub 2020 Sep 1.

Institute of Ageing and Chronic Disease, University of Liverpool, Liverpool, UK.

A range of bioreactors use linear actuators to apply tensile forces , but differences in their culture environments can limit a direct comparison between studies. The widespread availability of 3D printing now provides an opportunity to develop a 'universal' bioreactor chamber that, with minimal exterior editing can be coupled to a wide range of commonly used linear actuator platforms, for example, the EBERS-TC3 and CellScale MCT6, resulting in a greater comparability between results and consistent testing of potential therapeutics. We designed a bioreactor chamber with six independent wells that was 3D printed in polylactic acid using an Ultimaker 2+ and waterproofed using a commercially available coating (XTC-3D), an oxirane resin. Read More

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September 2020

A New Non-invasive Technique for Measuring 3D-Oxygen Gradients in Wells During Mammalian Cell Culture.

Front Bioeng Biotechnol 2020 17;8:595. Epub 2020 Jun 17.

cBITE, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, Netherlands.

Oxygen tension plays an important role in overall cell function and fate, regulating gene expression, and cell differentiation. Although there is extensive literature available that supports the previous statement, little information is to be found about accurate O measurements during culture. In fact, O concentration at the cell layer during culture is commonly assumed to be equal to that of the incubator atmosphere. Read More

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Low-Cost Automated Vectors and Modular Environmental Sensors for Plant Phenotyping.

Sensors (Basel) 2020 Jun 11;20(11). Epub 2020 Jun 11.

Integrated Phenomics Group, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington LE12 5RD, UK.

High-throughput plant phenotyping in controlled environments (growth chambers and glasshouses) is often delivered via large, expensive installations, leading to limited access and the increased relevance of "affordable phenotyping" solutions. We present two robot vectors for automated plant phenotyping under controlled conditions. Using 3D-printed components and readily-available hardware and electronic components, these designs are inexpensive, flexible and easily modified to multiple tasks. Read More

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Biocompatible PEGDA Resin for 3D Printing.

ACS Appl Bio Mater 2020 Apr 27;3(4):2239-2244. Epub 2020 Feb 27.

Chemical Engineering Department, Brigham Young University, Provo, Utah, USA 84602.

We report a non-cytotoxic resin compatible with and designed for use in custom high-resolution 3D printers that follow the design approach described in Gong et al., Lab Chip 17, 2899 (2017). The non-cytotoxic resin is based on a poly(ethylene glycol) diacrylate (PEGDA) monomer with avobenzone as the UV absorber instead of 2-nitrophenyl phenyl sulfide (NPS). Read More

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Mix-Bricks and Flip-Lids: 3D Printed Devices for Simple, Simultaneous Mixing of Reactant Solutions.

Anal Chem 2020 03 19;92(5):3522-3527. Epub 2020 Feb 19.

Chemistry Department, Hillsdale College, 33 East College Street, Hillsdale, Michigan 49242, United States.

Two types of 3D printed devices for simultaneous mixing of small volumes (e.g., 50-500 μL) of reactant solutions are described. Read More

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Development of an instrumented thoracoscopic surgical trainer for objective evaluation of esophageal atresia/tracheoesophageal fistula repair.

Med Biol Eng Comput 2020 Mar 11;58(3):601-609. Epub 2020 Jan 11.

Department of Paediatric Surgery, Christchurch Hospital, Christchurch, New Zealand.

Operative repair of complex conditions such as esophageal atresia and tracheoesophageal fistula (EA/TEF) is technically demanding, but few training opportunities exist outside the operating theater for surgeons to attain these skills. Learning them during surgery on actual neonates where the stakes are high, margins for error narrow, and where outcomes are influenced by technical expertise, is problematic. There is an increasing demand for high-fidelity simulation that can objectively measure performance. Read More

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Standardized mounting method of (zebrafish) embryos using a 3D-printed stamp for high-content, semi-automated confocal imaging.

BMC Biotechnol 2019 10 22;19(1):68. Epub 2019 Oct 22.

Department of Developmental Biology of Vertebrates, Institute for Cell biology and Neuroscience, Goethe University, Max-von-Laue-Str. 13, 60438, Frankfurt am Main, Germany.

Background: Developmental biology relies to a large extent on the observation and comparison of phenotypic traits through time using high resolution microscopes. In this context, transparent model organisms such as the zebrafish Danio rerio in which developing tissues and organs can be easily observed and imaged using fluorescent proteins have become very popular. One limiting factor however is the acquisition of a sufficient amount of data, in standardized and reproducible conditions, to allow robust quantitative analysis. Read More

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October 2019

Mri-based cancer lesion analysis with 3d printed patient specific prostate cutting guides.

Am J Clin Exp Urol 2019 15;7(4):215-222. Epub 2019 Aug 15.

Mechanical Engineering, University of Wisconsin Madison, WI, United States.

MRI methods have improved diagnosis and treatment planning for prostate cancer. However, validation and standardization is needed to encourage widespread adoption of these methods. The purpose of this study was to improve validation methods by creating a prostate cutting guide and to develop a method for 3D comparison between MRI data and post-prostatectomy histological tissue slices. Read More

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Easy calibration of the Light Plate Apparatus for optogenetic experiments.

MethodsX 2019 13;6:1480-1488. Epub 2019 Jun 13.

Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, United States.

Optogenetic systems use genetically-encoded light-sensitive proteins to control and study cellular processes. As the number and quality of these systems grows, there is an increasing need for user-friendly and flexible hardware to provide programmed illumination to cultures of cells. One platform which satisfies this need for a variety of optogenetic systems and organisms is the Light Plate Apparatus (LPA), which delivers a controlled light dose to each well of a 24-well plate. Read More

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Quantitative characterization of 3D bioprinted structural elements under cell generated forces.

Nat Commun 2019 07 10;10(1):3029. Epub 2019 Jul 10.

University of Florida, Herbert Wertheim College of Engineering, Department of Mechanical and Aerospace Engineering, Gainesville, FL, 32611, USA.

With improving biofabrication technology, 3D bioprinted constructs increasingly resemble real tissues. However, the fundamental principles describing how cell-generated forces within these constructs drive deformations, mechanical instabilities, and structural failures have not been established, even for basic biofabricated building blocks. Here we investigate mechanical behaviours of 3D printed microbeams made from living cells and extracellular matrix, bioprinting these simple structural elements into a 3D culture medium made from packed microgels, creating a mechanically controlled environment that allows the beams to evolve under cell-generated forces. Read More

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High-throughput multicolor optogenetics in microwell plates.

Nat Protoc 2019 07 24;14(7):2205-2228. Epub 2019 Jun 24.

Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA.

Optogenetic probes can be powerful tools for dissecting complexity in cell biology, but there is a lack of instrumentation to exploit their potential for automated, high-information-content experiments. This protocol describes the construction and use of the optoPlate-96, a platform for high-throughput three-color optogenetics experiments that allows simultaneous manipulation of common red- and blue-light-sensitive optogenetic probes. The optoPlate-96 enables illumination of individual wells in 96-well microwell plates or in groups of wells in 384-well plates. Read More

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Fabrication of a Lab-on-Chip Device Using Material Extrusion (3D Printing) and Demonstration via Malaria-Ab ELISA.

Micromachines (Basel) 2018 Jan 14;9(1). Epub 2018 Jan 14.

Department of Mechanical and Aerospace Engineering, University of California, Irvine, CA 92697, USA.

Additive manufacturing, such as fused deposition modeling (FDM), has been increasingly employed to produce microfluidic platforms due to ease of use, wide distribution of affordable 3D printers and relatively inexpensive materials for printing. In this work, we discuss fabrication and testing of an FDM-printed fully automated colorimetric enzyme-linked immunosorbent assay (ELISA) designed to detect malaria. The detection platform consists of a disposable 3D-printed fluidic cartridge (with elastomeric silicone domes on top of reagent-storage reservoirs) and a nondisposable frame with servomotors and electronic controls such as an Arduino board and a rechargeable battery. Read More

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January 2018

Self-filling microwell arrays (SFMAs) for tumor spheroid formation.

Lab Chip 2018 11;18(22):3516-3528

Biomaterials and Tissue Engineering Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran.

Tumor spheroid formation in microwell arrays is a promising approach for high-throughput screening of chemotherapeutic agents. This method offers the advantage of better mimicking the complexities of tumors as compared to conventional monolayer culture systems. However, using these technologies to their full potential is hindered by the inability to seed the cells within the wells uniformly and with high yield and reproducibility. Read More

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November 2018

Full Factorial Microfluidic Designs and Devices for Parallelizing Human Pluripotent Stem Cell Differentiation.

SLAS Technol 2019 02 11;24(1):41-54. Epub 2018 Jul 11.

1 Department of Otolaryngology and Head and Neck Surgery, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.

Human pluripotent stem cells (hPSCs) are promising therapeutic tools for regenerative therapies and disease modeling. Differentiation of cultured hPSCs is influenced by both exogenous factors added to the cultures and endogenously secreted molecules. Optimization of protocols for the differentiation of hPSCs into different cell types is difficult because of the many variables that can influence cell fate. Read More

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February 2019

Fabrication of Custom Agarose Wells for Cell Seeding and Tissue Ring Self-assembly Using 3D-Printed Molds.

J Vis Exp 2018 04 2(134). Epub 2018 Apr 2.

Biomedical Engineering, Worcester Polytechnic Institute;

Engineered tissues are being used clinically for tissue repair and replacement, and are being developed as tools for drug screening and human disease modeling. Self-assembled tissues offer advantages over scaffold-based tissue engineering, such as enhanced matrix deposition, strength, and function. However, there are few available methods for fabricating 3D tissues without seeding cells on or within a supporting scaffold. Read More

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An Affordable and Portable Thermocycler for Real-Time PCR Made of 3D-Printed Parts and Off-the-Shelf Electronics.

Anal Chem 2018 05 13;90(9):5563-5568. Epub 2018 Apr 13.

Unidad Monterrey, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional , Parque PIIT, Apodaca , Nuevo León C.P. 66628 , Mexico.

The polymerase chain reaction (PCR) is a sought-after nucleic acid amplification technique used in the detection of several diseases. However, one of the main limitations of this and other nucleic acid amplification assays is the complexity, size, maintenance, and cost of their operational instrumentation. This limits the use of PCR applications in settings that cannot afford the instruments but that may have access to basic electrical, electronic, and optical components and the expertise to build them. Read More

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3D printed hyperelastic "bone" scaffolds and regional gene therapy: A novel approach to bone healing.

J Biomed Mater Res A 2018 04 11;106(4):1104-1110. Epub 2018 Jan 11.

Department of Orthopaedic Surgery, Keck School of Medicine of the University of Southern California, 2011 Zonal Ave, HMR 702, Los Angeles, California, 90089.

The purpose of this study was to evaluate the viability of human adipose-derived stem cells (ADSCs) transduced with a lentiviral (LV) vector to overexpress bone morphogenetic protein-2 (BMP-2) loaded onto a novel 3D printed scaffold. Human ADSCs were transduced with a LV vector carrying the cDNA for BMP-2. The transduced cells were loaded onto a 3D printed Hyperelastic "Bone" (HB) scaffold. Read More

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3D-Printed seed planter and well array for high-throughput seed germination screening.

Integr Biol (Camb) 2018 01;10(1):67-73

Institute for Clean Energy & Advanced Materials, Faculty of Materials & Energy, Southwest University, Chongqing 400715, China.

Seed germination is an important means of evaluating seed quality. In the present study, a well array for a seed germination experiment was designed and fabricated by 3D printing for the first time. Each hollow cone-shaped well can hold one seed, which not only prevented the seed from falling out of the well but also ensured that part of the seed was fully exposed to the sublayer of wet filter paper, allowing it to receive water for germination. Read More

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January 2018

An Updated Protocol for High Throughput Plant Tissue Sectioning.

Front Plant Sci 2017 4;8:1721. Epub 2017 Oct 4.

The Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Nottingham, United Kingdom.

Quantification of the tissue and cellular structure of plant material is essential for the study of a variety of plant sciences applications. Currently, many methods for sectioning plant material are either low throughput or involve free-hand sectioning which requires a significant amount of practice. Here, we present an updated method to provide rapid and high-quality cross sections, primarily of root tissue but which can also be readily applied to other tissues such as leaves or stems. Read More

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October 2017