20,052 results match your criteria 35s transcript

Atmospheric washout of S during single rain events - Implications for S sampling schemes.

J Environ Radioact 2021 Jun 9;237:106669. Epub 2021 Jun 9.

UFZ Helmholtz Centre for Environmental Research, Theodor-Lieser-Str. 4, 06120, Halle, Germany.

Cosmogenic radiosulfur (S; half-life: 87.4 days) is transferred with the rain to the groundwater (as SO) and can be used as residence time tracer for the detection of sub-yearly groundwater residence times. Due to the distinct but non-regular annual S pattern in precipitation, related data evaluation requires consideration of aS input function that is based on representative rain samples. Read More

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PtrMYB120 functions as a positive regulator of both anthocyanin and lignin biosynthetic pathway in a hybrid poplar.

Tree Physiol 2021 Jun 7. Epub 2021 Jun 7.

Department of Plant & Environmental New Resources, Kyung Hee University, Yongin 17104, Republic of Korea.

Both anthocyanins and lignins are essential secondary metabolites in plant growth and development. Their biosynthesis is metabolically interconnected and diverges in the central metabolite 4-coumaroyl CoA of the phenylpropanoid pathway. Considerable progress has been made in understanding transcriptional regulation of genes involved in lignin and anthocyanin synthesis pathways, but the concerted regulation of these pathways is not yet fully understood. Read More

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Synthetic promoter designs enabled by a comprehensive analysis of plant core promoters.

Nat Plants 2021 Jun 3. Epub 2021 Jun 3.

Department of Genome Sciences, University of Washington, Seattle, WA, USA.

Targeted engineering of plant gene expression holds great promise for ensuring food security and for producing biopharmaceuticals in plants. However, this engineering requires thorough knowledge of cis-regulatory elements to precisely control either endogenous or introduced genes. To generate this knowledge, we used a massively parallel reporter assay to measure the activity of nearly complete sets of promoters from Arabidopsis, maize and sorghum. Read More

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An Agrobacterium-mediated stable transformation technique for the hornwort model Anthoceros agrestis.

New Phytol 2021 Jun 2. Epub 2021 Jun 2.

Department of Life Science, Rikkyo University, Tokyo, 171-8501, Japan.

Despite their key phylogenetic position and their unique biology, hornworts have been widely overlooked. Until recently there was no hornwort model species amenable to systematic experimental investigation. Anthoceros agrestis has been proposed as the model species to study hornwort biology. Read More

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PIP2, An Auxin Induced Plant Peptide Hormone Regulates Root and Hypocotyl Elongation in .

Front Plant Sci 2021 14;12:646736. Epub 2021 May 14.

Laboratory of Plant Molecular Genetics & Crop Gene Editing, School of Life Sciences, Linyi University, Linyi, China.

Auxin is one of the traditional plant hormones, whereas peptide hormones are peptides with hormone activities. Both auxin and plant peptide hormones regulate multiple aspects of plant growth and development, and there are cross-talks between auxin and plant peptide hormones. PAMP-INDUCED SECRETED PEPTIDES (PIPs) and PIP-LIKEs (PIPLs) are a new family of plant peptide hormone, and PIPL3/TARGET OF LBD SIXTEEN 2 (TOLS2) has been shown to regulate lateral root formation in . Read More

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Mdm-MIR393b-mediated adventitious root formation by targeted regulation of MdTIR1A expression and weakened sensitivity to auxin in apple rootstock.

Plant Sci 2021 Jul 22;308:110909. Epub 2021 Apr 22.

College of Horticulture, Yangling Subsidiary Center Project of the National Apple Improvement Center, Northwest Agriculture & Forestry University, Yangling, 712100, China. Electronic address:

Adventitious root (AR) formation is of great significance for apple rootstock breeding. It is widely accepted that miR393 influences AR formation in many plant species; however, the molecular mechanism by which factors regulate AR formation remains insufficient. In this study, the evolutionary relationship of mdm-miR393 and candidate target genes MdTIR1/AFB was systematically identified, and the expression patterns were analysed. Read More

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Overexpression of the CaHB12 transcription factor in cotton (Gossypium hirsutum) improves drought tolerance.

Plant Physiol Biochem 2021 Aug 17;165:80-93. Epub 2021 May 17.

Embrapa Genetic Resources and Biotechnology, Brasília, DF, 70297-400, Brazil; National Institute of Science and Technology, INCT PlantStress Biotech, EMBRAPA, Brasília, DF, 70297-400, Brazil; Catholic University of Brasília, Brasília, DF, 71966-700, Brazil. Electronic address:

The Coffea arabica HB12 gene (CaHB12), which encodes a transcription factor belonging to the HD-Zip I subfamily, is upregulated under drought, and its constitutive overexpression (35S:CaHB12) improves the Arabidopsis thaliana tolerance to drought and salinity stresses. Herein, we generated transgenic cotton events constitutively overexpressing the CaHB12 gene, characterized these events based on their increased tolerance to water deficit, and exploited the gene expression level from the CaHB12 network. The segregating events Ev8. Read More

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Genome-wide (ChIP-seq) identification of target genes regulated by WRKY33 during submergence stress in Arabidopsis.

BMC Genom Data 2021 05 24;22(1):16. Epub 2021 May 24.

Key Laboratory for Bio-resources and Eco-environment & State Key Lab of Hydraulics & Mountain River Engineering, College of Life Science, Sichuan University, Chengdu, 610065, China.

Background: Hypoxia induced by flooding causes significant losses to crop production almost every year. However, the molecular network of submergence signaling pathway is still poorly understood. According to previous studies, transgenic plants overexpressing the WRKY33 gene showed enhanced resistance to submergence stress. Read More

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Transgenic , Containing Individual Genes and Open Reading Frames Under Promoter, Exhibit Compact Habit, Reduced Plant Growth, and Altered Ethylene Tolerance in Flowers.

Front Plant Sci 2021 7;12:672023. Epub 2021 May 7.

Section for Crop Sciences, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Taastrup, Denmark.

Reduced growth habit is a desirable trait for ornamental potted plants and can successfully be obtained through transformation in a stable and heritable manner. Additionally, it can also be obtained by transformation with harboring specific genes from . The bacterial T-DNA harbors four () genes and 14 less known open reading frames (ORFs). Read More

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Development of marker-free transgenic pigeon pea (Cajanus cajan) expressing a pod borer insecticidal protein.

Sci Rep 2021 May 18;11(1):10543. Epub 2021 May 18.

Advanced Laboratory for Plant Genetic Engineering (ALPGE), IIT-Kharagpur, Kharagpur, India.

Pigeon pea, a grain legume of the semiarid tropics, is a rich source of high-quality protein. The productivity of this pulse is seriously affected by lepidopteron insect pests. To generate a sustainable insect-resistant plant, synthetically prepared bioactive key constituents of a crystal protein (Syn Cry1Ab) of Bacillus thuringiensis were expressed in pigeon pea under the guidance of a tissue-specific promoter of the RuBP carboxylase/oxygenase small subunit (rbcS) gene. Read More

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Substrate control of sulphur utilisation and microbial stoichiometry in soil: Results of C, N, C, and S quad labelling.

ISME J 2021 May 11. Epub 2021 May 11.

School of Natural Sciences, Bangor University, Gwynedd, UK.

Global plant sulphur (S) deficiency is increasing because of a reduction in sulphate-based fertiliser application combined with continuous S withdrawal during harvest. Here, we applied C, N, C, and S quad labelling of the S-containing amino acids cysteine (Cys) and methionine (Met) to understand S cycling and microbial S transformations in the soil. The soil microorganisms absorbed the applied Cys and Met within minutes and released SO within hours. Read More

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Characterization of a transcription factor SlNAC7 gene from Suaeda liaotungensis and its role in stress tolerance.

J Plant Res 2021 May 8. Epub 2021 May 8.

School of Life Sciences, Liaoning Normal University, Dalian, 116081, China.

NAC (NAM, ATAF1/2, CUC2) transcription factors play important roles in plant growth, development, and responses to abiotic stress. In this study, we cloned an NAC2 subfamily transcription factor gene (SlNAC7) from the halophyte Suaeda liaotungensis K., and conducted a series of studies to determine the characteristics and functions of this gene. Read More

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The barley SHN1-type transcription factor HvSHN1 imparts heat, drought and salt tolerances in transgenic tobacco.

Plant Physiol Biochem 2021 Jul 1;164:44-53. Epub 2021 May 1.

Laboratory of Plant Biotechnology and Improvement, University of Sfax, Center of Biotechnology of Sfax, Route Sidi Mansour, Km 6 B.P' 1177, 3018 Sfax, Tunisia. Electronic address:

The APETAL2/Ethylene Responsive Factor (AP2/ERF) family was the subject of intensive research which led to the identification of several members involved in different stress responses such as salinity, drought and high temperature. The SHN/WIN clade of AP2/ERF participates in many important processes such as cutin and wax biosynthesis, ethylene signaling and gene expression. Here, we report the functional analysis of SHN1-type transcription factor, HvSHN1, from barely. Read More

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A Novel Model Based on Deep Convolutional Neural Network Improves Diagnostic Accuracy of Intramucosal Gastric Cancer (With Video).

Front Oncol 2021 20;11:622827. Epub 2021 Apr 20.

Department of Gastroenterology, Nanjing Drum Tower Hospital, Affiliated Drum Tower Hospital, Medical School of Nanjing University, Nanjing, China.

Background And Aims: Prediction of intramucosal gastric cancer (GC) is a big challenge. It is not clear whether artificial intelligence could assist endoscopists in the diagnosis.

Methods: A deep convolutional neural networks (DCNN) model was developed retrospectively collected 3407 endoscopic images from 666 gastric cancer patients from two Endoscopy Centers (training dataset). Read More

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Correction to: Functional approaches to the study of G-protein-coupled receptors in postmortem brain tissue: [S]GTPγS binding assays combined with immunoprecipitation.

Pharmacol Rep 2021 May 6. Epub 2021 May 6.

Department of Pharmacology, Centro de Investigación Biomédica en Red de Salud Mental CIBERSAM, University of the Basque Country UPV/EHU, 48940, Leioa, Bizkaia, Spain.

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Bioengineering potato plants to produce benzylglucosinolate for improved broad-spectrum pest and disease resistance.

Transgenic Res 2021 May 6. Epub 2021 May 6.

Department of Plant and Environmental Sciences, DynaMo Center, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark.

In traditional, small-scale agriculture in the Andes, potatoes are frequently co-cultivated with the Andean edible tuber Tropaeolum tuberosum, commonly known as mashua, which is believed to exert a pest and disease protective role due to its content of the phenylalanine-derived benzylglucosinolate (BGLS). We bioengineered the production of BGLS in potato by consecutive generation of stable transgenic events with two polycistronic constructs encoding for expression of six BGLS biosynthetic genes from Arabidopsis thaliana. First, we integrated a polycistronic construct coding for the last three genes of the pathway (SUR1, UGT74B1 and SOT16) into potato driven by the cauliflower mosaic virus 35S promoter. Read More

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Designing a Graphic Novel: Engaging Community, Arts, and Culture Into Public Health Initiatives.

Health Promot Pract 2021 05;22(1_suppl):35S-43S

Emory University Nell Hodgson Woodruff School of Nursing, Atlanta, GA, USA.

The opioid epidemic was declared a national public health emergency in 2017. In Georgia, standing orders for the opioid antagonist, naloxone, have been implemented to reduce mortality from opioid overdoses. Service industry workers in the Atlanta, Georgia, inner-city community of Little Five Points (L5P) have access to naloxone, potentially expanding overdose rescue efforts in the community setting. Read More

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Molecular Cloning and Functional Analysis of 1-Deoxy-D-Xylulose 5-Phosphate Reductoisomerase from .

Genes (Basel) 2021 Apr 22;12(5). Epub 2021 Apr 22.

Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.

Sandalwood ( L.) heartwood-derived essential oil contains a high content of sesquiterpenoids that are economically highly valued and widely used in the fragrance industry. Sesquiterpenoids are biosynthesized via the mevalonate acid and methylerythritol phosphate (MEP) pathways, which are also the sources of precursors for photosynthetic pigments. Read More

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BAC- and oligo-FISH mapping reveals chromosome evolution among Vigna angularis, V. unguiculata, and Phaseolus vulgaris.

Chromosoma 2021 Apr 28. Epub 2021 Apr 28.

Departamento de Genética, Universidade Federal de Pernambuco, Recife, PE, Brazil.

Cytogenomic resources have accelerated synteny and chromosome evolution studies in plant species, including legumes. Here, we established the first cytogenetic map of V. angularis (Va, subgenus Ceratotropis) and compared this new map with those of V. Read More

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Systematic evaluation of a panel of 30 synthetic cannabinoid receptor agonists structurally related to MMB-4en-PICA, MDMB-4en-PINACA, ADB-4en-PINACA, and MMB-4CN-BUTINACA using a combination of binding and different CB1 receptor activation assays. Part III: The G protein pathway and critical comparison of different assays.

Drug Test Anal 2021 Apr 27. Epub 2021 Apr 27.

Institute of Forensic Medicine, Forensic Toxicology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.

The present work is the last of a three-part study investigating a panel of 30 systematically designed synthetic cannabinoid receptor agonists (SCRAs) including features such as the 4-pentenyl tail and varying head groups including amides and esters of l-valine (MMB, AB), l-tert-leucine (ADB), and l-phenylalanine (APP), as well as adamantyl (A) and cumyl moieties (CUMYL). Here, we evaluated these SCRAs for their capacity to activate the human cannabinoid receptor 1 (CB ) via indirect measurement of G protein recruitment. Furthermore, we comparatively evaluated the results obtained from three in vitro assays, based on the recruitment of β-arrestin 2 (βarr2 assay) or Gα protein (mini-Gα assay), or binding of [ S]-GTPγS. Read More

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Evaluation of [Cys(ATTO 488)8]Dermorphin-NH2 as a novel tool for the study of μ-opioid peptide receptors.

PLoS One 2021 23;16(4):e0250011. Epub 2021 Apr 23.

Department of Cardiovascular Sciences (Anaesthesia, Critical Care and Pain Management), University of Leicester, Leicester Royal Infirmary, Leicester, United Kingdom.

The μ-opioid peptide (MOP) receptor is a member of the opioid receptor family and an important clinical target for analgesia. Measuring MOP receptor location and tracking its turnover traditionally used radiolabels or antibodies with attendant problems of utility of radiolabels in whole cells and poor antibody selectivity. To address these issues we have synthesized and characterised a novel ATTO488 based fluorescent Dermorphin analogue; [Cys(ATTO 488)8]Dermorphin-NH2 (DermATTO488). Read More

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Functional approaches to the study of G-protein-coupled receptors in postmortem brain tissue: [S]GTPγS binding assays combined with immunoprecipitation.

Pharmacol Rep 2021 Apr 20. Epub 2021 Apr 20.

Department of Pharmacology, Centro de Investigación Biomédica en Red de Salud Mental, University of the Basque Country UPV/EHU CIBERSAM, 48940, Leioa, Bizkaia, Spain.

G-protein-coupled receptors (GPCRs) have an enormous biochemical importance as they bind to diverse extracellular ligands and regulate a variety of physiological and pathological responses. G-protein activation measures the functional consequence of receptor occupancy at one of the earliest receptor-mediated events. Receptor coupling to G-proteins promotes the GDP/GTP exchange on Gα subunits. Read More

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Tobacco transcription factor bHLH123 improves salt tolerance by activating NADPH oxidase NtRbohE expression.

Plant Physiol 2021 Apr 19. Epub 2021 Apr 19.

College of Horticulture, Qingdao Agricultural University, Qingdao 266101, Shandong Province, China.

In plants, reactive oxygen species (ROS) produced following the expression of the respiratory burst oxidase homolog (Rboh) gene are important regulators of stress responses. However, little is known about how plants acclimate to salt stress through the Rboh-derived ROS signaling pathway. Here, we showed that a 400-bp fragment of the tobacco (Nicotiana tabacum) NtRbohE promoter played a critical role in the salt response. Read More

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Origin and evolution of highly polymorphic rDNA sites in (Alstroemeriaceae) and related species.

Genome 2021 Apr 14. Epub 2021 Apr 14.

Universidade Federal de Pernambuco, Recife, Brazil;

(Alstroemeriaceae) displays a conserved and highly asymmetric karyotype, where most rDNA sites can be properly recognized by the size and morphology of the chromosomes. We analyzed the intraspecific variation of rDNA sites in and compared with their distribution in other species ( and ) and a representative of the sister genus, Bomarea edulis. All three species presented 2n = 16 and one to six B chromosomes were found in some individuals of . Read More

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Moniliophthora perniciosa, the causal agent of witches' broom disease of cacao, interferes with cytokinin metabolism during infection of Micro-Tom tomato and promotes symptom development.

New Phytol 2021 07 13;231(1):365-381. Epub 2021 May 13.

Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Av. Centenário 303, Piracicaba, SP, 13400-970, Brazil.

Moniliophthora perniciosa causes witches' broom disease of cacao and inflicts symptoms suggestive of hormonal imbalance. We investigated whether infection of the tomato (Solanum lycopersicum) model system Micro-Tom (MT) by the Solanaceae (S)-biotype of Moniliophthora perniciosa, which causes stem swelling and hypertrophic growth of axillary shoots, results from changes in host cytokinin metabolism. Inoculation of an MT-transgenic line that overexpresses the Arabidopsis CYTOKININ OXIDASE-2 gene (35S::AtCKX2) resulted in a reduction in disease incidence and stem diameter. Read More

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Development of a Systematic qPCR Array for Screening GM Soybeans.

Foods 2021 Mar 13;10(3). Epub 2021 Mar 13.

Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Korea.

A screening method using the 35S promoter and nos terminator for genetically modified organisms (GMOs) is not sufficient to cover all GM soybean events. In this study, a real-time polymerase chain reaction (also known as quantitative polymerase chain reaction, qPCR) array targeting eight screening assays combined with a prediction system was developed for the rapid tracking of GM soybeans. Each assay's specificity was tested and confirmed using 17 GM soybean events that have been approved in Korea. Read More

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Correction: Rodionov, A.V., et al. Intragenomic Polymorphism of the ITS 1 Region of 35S rRNA Gene in the Group of Grasses with Two-Chromosome Species: Different Genome Composition in Closely Related Species. 2020, , 1647.

Plants (Basel) 2021 Mar 1;10(3). Epub 2021 Mar 1.

Laboratory of Biosystematics and Cytology, Komarov Botanical Institute of the Russian Academy of Sciences, 197376 St. Petersburg, Russia.

The authors wish to make the following corrections to their paper [... Read More

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Isolation and Functional Characterization of a Salt-Responsive Calmodulin-Like Gene from Semi-Mangrove .

Int J Mol Sci 2021 Mar 27;22(7). Epub 2021 Mar 27.

Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.

Salt stress is a major increasing threat to global agriculture. Pongamia (), a semi-mangrove, is a good model to study the molecular mechanism of plant adaptation to the saline environment. Calcium signaling pathways play critical roles in the model plants such as Arabidopsis in responding to salt stress, but little is known about their function in Pongamia. Read More

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The gibberellin signaling negative regulator RGA-LIKE3 promotes seed storage protein accumulation.

Plant Physiol 2021 Apr;185(4):1697-1707

Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Innovative Academy of Seed Design, Chinese Academy of Sciences, Guangzhou 510650, China.

Seed storage protein (SSP) acts as one of the main components of seed storage reserves, of which accumulation is tightly mediated by a sophisticated regulatory network. However, whether and how gibberellin (GA) signaling is involved in this important biological event is not fully understood. Here, we show that SSP content in Arabidopsis (Arabidopsis thaliana) is significantly reduced by GA and increased in the GA biosynthesis triple mutant ga3ox1/3/4. Read More

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Expression of a Bacterial Trehalose-6-phosphate Synthase otsA Increases Oil Accumulation in Plant Seeds and Vegetative Tissues.

Front Plant Sci 2021 10;12:656962. Epub 2021 Mar 10.

Department of Biology, Brookhaven National Laboratory, Upton, NY, United States.

We previously demonstrated that exogenous trehalose 6-phosphate (T6P) treatment stabilized WRINKLED1 (WRI1), a master transcriptional regulator of fatty acid (FA) synthesis and increased total FA content in () embryo suspension cell culture. Here, we explore lines heterologously expressing the T6P synthase (otsA) or T6P phosphatase (otsB) to refine our understanding regarding the role of T6P in regulating fatty acid synthesis both in seeds and vegetative tissues. 35S: transgenic seeds showed an increase of 13% in fatty acid content compared to those of wild type (WT), while seeds of 35: transgenic seeds showed a reduction of 12% in fatty acid content compared to WT. Read More

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