2,140 results match your criteria 3 Biotech[Journal]


Genome-wide selective sweep analysis of the high-altitude adaptability of yaks by using the copy number variant.

3 Biotech 2020 Jun 18;10(6):259. Epub 2020 May 18.

3Sichuan Academy of Grassland Sciences, Chengdu, 611731 Sichuan China.

The domestic yak () from the Qinghai-Tibet Plateau is an important animal model in high-altitude adaptation studies. Here, we performed the genome-wide selective sweep analysis to identify the candidate copy number variation (CNV) for the high-altitude adaptation of yaks. A total of 531 autosomal CNVs were determined from 29 yak genome-wide resequencing data (15 high- and 14 low-altitude distributions) by using a CNV caller with a CNV identification interval > 5 kb, CNV silhouette score > 0. Read More

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http://dx.doi.org/10.1007/s13205-020-02254-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235113PMC

Identification, genetic diversity and recombination analysis of  isolates.

3 Biotech 2020 Jun 17;10(6):257. Epub 2020 May 17.

3Department of Biotechnology, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, UP India.

 (WMV) is an important virus causing adverse effects on cucurbits throughout the world. In this study, we recorded WMV infection in the watermelon (-growing area of Alwar and Sikar in districts of Rajasthan, India. The RT-PCR-based detection was performed to confirm the presence of WMV, by using potyvirus-degenerated coat protein primers. Read More

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http://dx.doi.org/10.1007/s13205-020-02248-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231810PMC

Elicitation and enhancement of bacoside production using suspension cultures of (L.) Wettst.

3 Biotech 2020 Jun 16;10(6):256. Epub 2020 May 16.

School of Biotechnology, Shri Mata Vaishno Devi University, Kakryal, Katra, Jammu and Kashmir 182320 India.

The effect of different elicitors [jasmonic acid, salicylic acid] and precursors [calcium pantothenate, cholesterol, sodium nitroprusside] on the stimulation of biomass and secondary metabolite production in suspension cultures of was studied. Induction of primary callus cultures was successfully carried out on the Gamborg's B (B) medium fortified with 2, 4-D (1.0 mg l) using leaves as explants. Read More

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http://dx.doi.org/10.1007/s13205-020-02242-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230074PMC

Two light responsive genes exhibit positive and negative correlation with picroside content in Royle ex Benth, an endangered medicinal herb.

3 Biotech 2020 Jun 16;10(6):255. Epub 2020 May 16.

1Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, P.O. Box No. 6, Palampur, Himachal Pradesh 176061 India.

is an endangered herb known to produce the medicinally important picrosides through isoprenoid pathway. The present work showed the functionality of WRKY motifs (TGAC -acting elements) present in the promoters of regulatory genes 3-hydroxy-3-methylglutaryl coenzyme A reductase () and 1-deoxy-d-xylulose-5-phosphate synthase () of the picrosides biosynthetic pathway by electrophoretic mobility shift assay. Also, the two genes, and were characterized and found to contain double and single characteristic WRKY domains, respectively along with a zinc-finger motif in each domain. Read More

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http://dx.doi.org/10.1007/s13205-020-02249-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230116PMC

Sonohydrolysis using an enzymatic cocktail in the preparation of free fatty acid.

3 Biotech 2020 Jun 15;10(6):254. Epub 2020 May 15.

1Instituto de Engenharias E Desenvolvimento Sustentável, Universidade da Integração Internacional da Lusofonia Afro-Brasileira, Campus da Auroras, Redenção, CE 62790970 Brazil.

In this work, the concept of lipase cocktail has been proposed in the ultrasound-assisted hydrolysis of coconut oil. Lipase from (TLL), lipase from (RML), and lipase B from (CALB) were evaluated as biocatalysts in different combinations. The best conversion (33. Read More

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http://dx.doi.org/10.1007/s13205-020-02227-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229052PMC

Production of 2-keto-gluconic acid from glucose by immobilized resting cells.

3 Biotech 2020 Jun 15;10(6):253. Epub 2020 May 15.

Jiangxi Provincial Engineering and Technology Center for Food Additives Bio-Production, Dexing, 334221 People's Republic of China.

2-Keto-d-gluconic acid (2KGA) is an important organic acid derived from d-glucose and is used to produce the food antioxidant erythorbic acid. To improve the 2KGA production performance and cell reusability, various carriers such as calcium alginate, k-carrageenan, chitosan, and poly(vinyl alcohol)-alginate were evaluated to immobilize JUIM01 resting cells. Calcium alginate was shown to be a suitable carrier since the immobilized cells had the highest number of reuse times and produced the highest 2KGA concentration of 171. Read More

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http://dx.doi.org/10.1007/s13205-020-02243-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229078PMC

Performance of a continuous stirred tank bioreactor employing an immobilized actinobacteria mixed culture for the removal of organophosphorus pesticides.

3 Biotech 2020 Jun 15;10(6):252. Epub 2020 May 15.

1Centro de Investigación Biotecnológica Aplicada al Medio Ambiente (CIBAMA-BIOREN), Universidad de La Frontera, Av. Francisco Salazar, 01145 Temuco, Chile.

In this study, we evaluated polyurethane foam (PF), volcanic rock (VR), and a modified plastic cap (MPC) as supports for the immobilization of organophosphorus (OP) pesticide-degrading actinobacterial strains. The colonization and activity of four streptomycetes were favoured by PF, which was selected as the carrier to use in a continuous stirred tank bioreactor (CSTR) that can be operated at increasing inflows of a pesticide mixture that contains the insecticides chlorpyrifos (CP) and diazinon (DZ). Our results demonstrate that the CSTR can be operated at flow rates of 10 and 40 mL h with greater than 85% removal of the pesticides in the short term. Read More

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http://dx.doi.org/10.1007/s13205-020-02239-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229079PMC

Role of radical quenching activity of dihydrocanaric acid in the treatment of cancer-experimental and theoretical.

3 Biotech 2020 Jun 13;10(6):251. Epub 2020 May 13.

1Department of Oncogene Regulation, Chittaranjan National Cancer Institute, 37, S.P.Mukherjee Road, Kolkata, 700 026 India.

In the present study, we have experimentally and theoretically studied the free-radical quenching property of dihydrocanaric acid (DCA) isolated from seedpods of . A modified method was used to estimate the nitric oxide scavenging effect of the DCA (significant activity of 75.22%) along with methanolic extract of seed pods of (72. Read More

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http://dx.doi.org/10.1007/s13205-020-02221-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221048PMC

Reverse transcriptase loop-mediated isothermal amplification and reverse transcriptase recombinase amplification assays for rapid and sensitive detection of cardamom vein clearing virus.

Authors:
K P Naveen A I Bhat

3 Biotech 2020 Jun 12;10(6):250. Epub 2020 May 12.

Division of Crop Protection, ICAR-Indian Institute of Spices Research, Kozhikode, 67012 Kerala India.

In the present study, two isothermal molecular assays viz reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) and reverse transcriptase recombinase amplification (RT-RPA) were developed to detect the cardamom vein clearing virus (CdVCV) infecting cardamom. Assays were optimized for parameters like duration, temperature and concentration of magnesium sulfate, and betaine in the case of RT-LAMP and magnesium acetate in the case of RT-RPA. Detection limits of both assays were determined and compared with conventional RT-PCR and SYBR Green-based real-time RT-PCR. Read More

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http://dx.doi.org/10.1007/s13205-020-02238-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218033PMC

Biodegradation of aromatic hydrocarbons using microbial adsorbed bioreactor.

Authors:
Dhanya Vijayan

3 Biotech 2020 Jun 11;10(6):249. Epub 2020 May 11.

Department of Biotechnology, Al-Ameen College, Edathala, Aluva, Ernakulam, Kerala 683564 India.

Polyurethane (PU) tubular coil-based bioreactor was constructed and evaluated for the effective biodegradation of benzene, toluene, xylene and phenol (BTXP). Herein, the removal of BTXP was done with a formulated bacterial consortium adsorbed on the inner surface of the PU coil. The formulated consortium consisted of four bacterial strains namely, sp , sp. Read More

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http://dx.doi.org/10.1007/s13205-020-02236-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7214589PMC

Inhibition of pathogenic using calamenene, derived from the Indian gorgonian , and its synthetic analog.

3 Biotech 2020 Jun 11;10(6):248. Epub 2020 May 11.

1Council of Scientific and Industrial Research (CSIR)-National Institute of Oceanography (NIO), Regional Centre, Cochin, Kerala 682018 India.

We report the synthesis and antimicrobial properties of a partially reduced dihydronathphthoquinone analogue of 2-methoxy, 5-acetoxy calamenene, extracted from . The growth of a pathogenic strain was effectively controlled by the calamenene derivative ( and its synthetic analog . Complete mortality of was observed with 2. Read More

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http://dx.doi.org/10.1007/s13205-020-02241-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7214568PMC

Amyloid beta peptide-degrading microbial enzymes and its implication in drug design.

3 Biotech 2020 Jun 11;10(6):247. Epub 2020 May 11.

1Structural Bioinformatics Unit, Department of Biochemistry, Shivaji University, Kolhapur, Maharashtra 416004 India.

Alzheimer's disease (AD) is a chronic and progressive neurological brain disorder. AD pathophysiology is mainly represented by formation of neuritic plaques and neurofibrillary tangles (NFTs). Neuritic plaques are made up of amyloid beta (Aβ) peptides, which play a central role in AD pathogenesis. Read More

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http://dx.doi.org/10.1007/s13205-020-02240-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7214582PMC

Expressions and clinical significances of STAT3 and Grim19 in epithelial ovarian cancer.

3 Biotech 2020 Jun 11;10(6):246. Epub 2020 May 11.

1Department of Gynaecology, Guangxi Medical University Affiliated Tumor Hospital, No. 71 Hedi Road, Nanning, 530021 China.

This study aimed to explore the expressions of signal transducer and activator of transcription 3 (STAT3) and a gene associated with retinoid-interferon induced mortality (Grim19) in epithelial ovarian cancer (EOC), and to determine their correlations with tumor progression and metastasis as well as the related mechanism. Ovarian tissue specimens resected through operation in our hospital were collected, and the correlations of Grim19 and STAT3 expressions with clinicopathological indexes were detected via immunohistochemistry (IHC) and Western blotting. Their positions in cells were observed through immunofluorescence. Read More

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http://dx.doi.org/10.1007/s13205-020-02237-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7214566PMC

Verbascoside production in long-term Kunth cell suspension cultures.

3 Biotech 2020 Jun 11;10(6):245. Epub 2020 May 11.

1Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, San Rafael Atlixco No. 186, Col. Vicentina, CP 09340 Mexico City, Mexico.

Previously, our group reported the establishment of a white callus cell line of Kunth that is a high producer of the secondary metabolite, verbascoside (VB, also named acteoside), under suspension culture conditions. Here, we present experimental evidence of the sustained ability of that cellular line to grow and produce high amounts of VB for 5 years of continuous culture. Cellular line profiles were determined at the early (at the beginning) and late stages (at the end of 5 years of continuous subculturing) by analyzing relevant parameters of culture growth, i. Read More

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http://dx.doi.org/10.1007/s13205-020-02222-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7214575PMC

Dexmedetomidine attenuates cisplatin-induced cognitive impairment by modulating miR-429-3p expression in rats.

3 Biotech 2020 Jun 10;10(6):244. Epub 2020 May 10.

Department of Anesthesiology, The Second Affiliated Hospital of Nanchang University, No. 1 Minde Road, Donghu District, Nanchang, 330006 Jiangxi Province China.

Chemotherapy-induced cognitive impairment (CICI) is widely recognized as a frequent adverse side effect following the administration of chemotherapeutic agents. This study aimed to explore the neuroprotective functions and mechanisms of microRNAs (miRNAs) mediated by dexmedetomidine (Dex) on cisplatin-induced CICI. The model rats received 5 mg/kg cisplatin injections once per week for 4 weeks. Read More

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http://dx.doi.org/10.1007/s13205-020-02217-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211776PMC

The complete genome sequence of LPL061, an exopolysaccharide-producing bacterium.

3 Biotech 2020 Jun 9;10(6):243. Epub 2020 May 9.

1Key Laboratory of Precision Nutrition and Food Quality, Key Laboratory of Functional Dairy, Ministry of Education; College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua East Road, East Campus, Haidian District, Beijing, 100083 China.

LPL061, which shows strong exopolysaccharide (EPS) producing capacity, was isolated from carnations in Beijing, China. The complete genome of LPL061 comprised a single circular chromosome (3,907,268 bp; G+C content of 46.7%) with 3,737 coding DNA sequences, 26 rRNA, and 89 tRNA. Read More

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http://dx.doi.org/10.1007/s13205-020-02228-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211227PMC

Draft genome of five strains: agave, maize and sorghum plant-associated bacteria with resistance to metals.

3 Biotech 2020 Jun 9;10(6):242. Epub 2020 May 9.

1Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Prol. Carpio y Plan de Ayala S/N. Col. Santo Tomás, Del. Miguel Hidalgo, C.P. 11340 Cd. de México, Mexico.

Five strains of , a metal-resistant, plant-associated bacterium, were selected for genome sequencing through the Genomic Encyclopedia of Bacteria and Archaea (GEBA) Phase IV project at the Joint Genome Institute (JGI) of the U.S. Department of Energy (DOE). Read More

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http://dx.doi.org/10.1007/s13205-020-02210-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211221PMC

In vitro prebiotic potential, digestibility and biocompatibility properties of laminari-oligosaccharides produced from curdlan by β-1,3-endoglucanase from .

3 Biotech 2020 Jun 9;10(6):241. Epub 2020 May 9.

Carbohydrate Enzyme Biotechnology Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039 India.

Curdlan or laminarin, a β-1,3-glucan was hydrolysed by β-1,3-endoglucanase (Lam81A) from to produce laminari-oligosaccharides. TLC analysis of hydrolysed curdlan showed the presence of laminari-oligosaccharides of the degree of polymerization, DP2-DP7. This mixture of laminari-oligosaccharides displayed prebiotic properties. Read More

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http://dx.doi.org/10.1007/s13205-020-02234-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211229PMC

Identification of (Apiaceae) seeds by allele-specific PCR based on ITS sequences.

3 Biotech 2020 Jun 8;10(6):240. Epub 2020 May 8.

1School of Traditional Chinese Medicine, Capital Medical University, 10 You'anmen Outer West 1st Street, Beijing, 100069 China.

The traditional Chinese medicine Bupleuri radix (chaihu) is the dried roots of and and many adulterants exist because of the differences in traditional understanding, medication habits and seed resources. Therefore, rapid and accurate identification methods for (Apiaceae) seeds are required. We analyzed the internal transcribed spacer (ITS) sequences of five common species to detect variations in them, including , , var, and var. Read More

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http://dx.doi.org/10.1007/s13205-020-02233-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7210332PMC

The enzymatic characters of heterologous expressed novel β-1, 4-glucosidase originated from .

3 Biotech 2020 Jun 8;10(6):239. Epub 2020 May 8.

State Key Laboratory of Animal Nutrition, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, 100193 China.

β-1, 4-glucosidases generate glucose from cellobiose and oligosaccharides, enhancing the productivity in biorefinery and the bioconversion process as well as the nutritional value in food and feed. With the high-throughput sequencing technique, a novel β-1, 4-glucosidase, named T2, containing 861 amino acid residues, was found from . T2 belongs to the glycosyl hydrolase (GH) family 3. Read More

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http://dx.doi.org/10.1007/s13205-020-02229-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7210331PMC

Unravelling the antibiotic and heavy metal resistome of a chronically polluted soil.

3 Biotech 2020 Jun 7;10(6):238. Epub 2020 May 7.

Microbiology Unit, Department of Biological Sciences, Summit University, Offa, Kwara Nigeria.

The antibiotic and heavy metal resistome of a chronically polluted soil (3S) obtained from an automobile workshop in Ilorin, Kwara State, Nigeria was deciphered via functional annotation of putative ORFs (open reading frames). Functional annotation of antibiotic and heavy metal resistance genes in 3S metagenome was conducted using the Comprehensive Antibiotic Resistance Database (CARD), Antibiotic Resistance Gene-annotation (ARG-ANNOT) and Antibacterial Biocide and Metal Resistance Gene Database (BacMet). Annotation revealed detection of resistance genes for 15 antibiotic classes with the preponderance of beta lactamases, mobilized colistin resistance determinant (mcr), glycopepetide and tetracycline resistance genes, the OqxBgb and OqxA RND-type multidrug efflux pumps, among others. Read More

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http://dx.doi.org/10.1007/s13205-020-02219-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7205953PMC

Extracellular synthesis of silver nanoparticles by and evaluation of their antibacterial and cytotoxic effects.

3 Biotech 2020 Jun 7;10(6):237. Epub 2020 May 7.

1Environmental Science and Engineering Department (ESED), IIT Bombay, Mumbai, 400076 India.

Extracellular biosynthesis of silver nanoparticles (AgNPs) was explored using since this strain exhibits both nitrate- and nitrite-reductase enzyme activity (NaR and NiR, respectively). Optimal AgNP synthesis was achieved using 2 mM AgNO, culture supernatant of nutrient broth grown and incubation at 37 °C and 180 rpm. Under these conditions, the localized surface plasmon resonance peak of silver at 404 nm matched well with the average size of the spherical AgNPs based on FEG-TEM micrographs, i. Read More

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http://dx.doi.org/10.1007/s13205-020-02218-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7205969PMC

Comparative analysis of drought-responsive transcriptomes of sugarcane genotypes with differential tolerance to drought.

3 Biotech 2020 Jun 7;10(6):236. Epub 2020 May 7.

Biotechnology Section, Division of Crop Improvement, Indian Council of Agricultural Research- Sugarcane Breeding Institute, Coimbatore, Tamil Nadu 641 007 India.

Water stress causes considerable yield losses in sugarcane. To investigate differentially expressed genes under water stress, two sugarcane genotypes were subjected to three water-deficit levels (mild, moderate, and severe) and subsequent recovery and leaf transcriptome was generated using Illumina NextSeq sequencing. Among the differentially expressed genes, the tolerant genotype Co 06022 generated 2970 unigenes ( ≤ 0. Read More

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http://dx.doi.org/10.1007/s13205-020-02226-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7203378PMC

Complete genome sequence of a new bipartite begomovirus associated with leaf curl disease of .

3 Biotech 2020 May 6;10(5):235. Epub 2020 May 6.

Plant RNAi Biology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067 India.

The complete bipartite genome, consisting of DNA-A and DNA-B, of a novel begomovirus isolate associated with apical leaf curling and crinkled leaf disease of Chili () from New Delhi, India was cloned and sequenced. The sequence of DNA-A (2737 nt) and DNA-B (2692 nt) of the virus was submitted to NCBI, USA under the accessions MK069591 & MG597211, respectively. Sequence identity of the common region (CR) and presence of identical iterons (GAGTG) between the DNA-A and DNA-B clones indicate that they constitute a related pair. Read More

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http://dx.doi.org/10.1007/s13205-020-02220-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7203235PMC

Inheritance and molecular mapping of powdery mildew () resistance gene(s) in sunflower ( L.).

3 Biotech 2020 May 6;10(5):234. Epub 2020 May 6.

ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad, Telangana 500 030 India.

Sources of resistance to powdery mildew incited by have been identified in wild sunflowers and few exotic lines. The present investigation has been undertaken to study the inheritance of powdery mildew resistance and to map the quantitative trait loci (QTLs) governing resistance to powdery mildew in a multiple disease resistance line, TX16R (PI 642072). The inheritance was observed as a continuous distribution in a set of 264 F population and 93 recombinant inbred lines (RILs) of a cross between a highly susceptible accession PS 2023 and TX16R. Read More

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http://dx.doi.org/10.1007/s13205-020-02224-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7203404PMC

Tertiary treatment ( sp.) of a mixed effluent from two secondary treatments (immobilized recombinant and rPOXA 1B concentrate) of coloured laboratory wastewater (CLWW).

3 Biotech 2020 May 6;10(5):233. Epub 2020 May 6.

4Laboratorio de Biotecnología Aplicada, Grupo de Biotecnología Ambiental e Industrial (GBAI), Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana (PUJ), Bogotá, D.C., Colombia.

Industrial development has increased wastewater (WW) volume; generating contamination and disturbing ecosystems, because of breeching disposal parameters. In this work, Coloured Laboratory Wastewater (CLWW), (1500.00 colour units, CU) was separately submitted to two secondary treatments. Read More

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http://dx.doi.org/10.1007/s13205-020-02232-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200962PMC

The draft genome of TRPF4, a bacteriocin producer with potent activity against the causative agent of Legionnaires' Disease.

3 Biotech 2020 May 6;10(5):232. Epub 2020 May 6.

1Departamento de Microbiologia, Laboratório de Biotecnologia e Biodiversidade Para o Meio Ambiente, Universidade Federal de Viçosa, Peter Henry Rolfs Avenue w/n, Viçosa, MG Brazil.

In this work, we present the draft genome sequence of strain TRPF4 consisting of 2,634,550 bp with a G + C content of 32.4%. The genome sequence includes 2466 protein-coding genes, 11 rRNAs and 62 tRNAs, in 33 contigs. Read More

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http://dx.doi.org/10.1007/s13205-020-02231-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200953PMC

Complete genome sequence and antimicrobial activity of JT3-1, a microbial germicide isolated from yak feces.

3 Biotech 2020 May 5;10(5):231. Epub 2020 May 5.

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046 People's Republic of China.

JT3-1 was isolated from feces of the domestic yak () in Gansu province of China. Aim to know whether JT3-1 has the potency to be developed as a probiotic bacterium, works on the complete genome sequence, antimicrobial activity, growth performance in calves, and treatment effect on calf diarrhea of JT3-1 were carried out. The results showed that the complete genome of JT3-1 contains one gapless circular chromosome which is 3,929,799 bp, and has 3761 protein-encoding genes with an average GC content of 46. Read More

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http://dx.doi.org/10.1007/s13205-020-02235-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200956PMC

Preparation, characterization, and anticancer efficacy of novel cobalt oxide nanoparticles conjugated with thiosemicarbazide.

3 Biotech 2020 May 5;10(5):230. Epub 2020 May 5.

1Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran.

Gastric cancer is one of the most common cancers in modern societies. Previous studies have shown that the use of nanoparticle complexes is effective in the treatment of cancer. The aim of this study was to investigate the cytotoxicity and anticancer properties of cobalt oxide (CoO) nanoparticles (NPs) functionalized by glutamic acid (Glu) and conjugated with thiosemicarbazide (TSC) on gastric cancer (AGS) cell line. Read More

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http://dx.doi.org/10.1007/s13205-020-02230-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200957PMC

Role of hydrogen cyanamide (HC) in grape bud dormancy release: proteomic approach.

3 Biotech 2020 May 5;10(5):229. Epub 2020 May 5.

1College of Horticulture, Nanjing Agricultural University, Nanjing, 210095 China.

In the present study, we identified changes in protein expression patterns of grapevine buds when treated with hydrogen cyanamide (HC). HC induced a shift of more than 2-folds in the expression of 1250 proteins out of approximately 7000 detected proteins. The majority of the differentially expressed proteins (DEPs) were localized in the chloroplast (419) and cytoplasm (347). Read More

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http://dx.doi.org/10.1007/s13205-020-02194-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200958PMC

Bacterial diversity in the intestinal mucosa of mice fed with Asparagus extract under high-fat diet condition.

3 Biotech 2020 May 4;10(5):228. Epub 2020 May 4.

2Hunan University of Chinese Medicine, Xueshi Road 300, Yuelu District, Changsha, 410208 Hunan Province China.

The purpose of this study is to determine the effect of Asparagus on bacterial diversity in the intestinal mucosa of mice fed with high-fat diet, thus providing theoretical basis for the development and research of Asparagus products. Twelve healthy male Kunming mice and twelve healthy female Kunming mice were chosen and randomly divided into normal group, model group, Asparagus group, and lipid-lowering decoction group, with six mice in each group. After establishing the models of mice fed with high-fat diet through feeding with high-fat diet, the mice in the Asparagus group were gavaged with Asparagus juice, those in the lipid-lowering decoction group were gavaged with lipid-lowering decoction, and those in the normal group and high-fat diet group were gavaged with the equal amount of distilled water. Read More

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http://dx.doi.org/10.1007/s13205-020-02225-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198693PMC

Targeted DNA complementation on a 1,1'-carbonyldiimidazole-functionalized surface for identifying .

3 Biotech 2020 May 2;10(5):227. Epub 2020 May 2.

Department of Tuberculosis Complications, Xi'an Chest Hospital, East Section of Aerospace Avenue, Chang'an District, Xi'an, 710100 Shaanxi China.

Herein, a rapid and sensitive current-volt measurement was developed for identifying the IS6110 DNA sequence to diagnose (TB). An aminated capture probe was immobilized on a 1,1'-carbonyldiimidazole-functionalized interdigitated electrode (IDE) silica substrate, and the target sequence was detected by complementation. It was found that all tested concentrations displayed a higher response in current changes than the control, and the limit of detection was 10 fM. Read More

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http://dx.doi.org/10.1007/s13205-020-02216-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7196115PMC

Arginine deaminase from PS2: purification, biochemical characterization and in-vitro evaluation of anticancer activity.

3 Biotech 2020 May 2;10(5):226. Epub 2020 May 2.

1Bacteriology Laboratory, Department of P.G. Studies and Research in Biological Science, Rani Durgavati University, Jabalpur, Madhya Pradesh India.

In the present study, arginine deaminase (ADI) was purified from PS2 which showed relative molecular mass of 70 ± 3 kDa on native-PAGE and 36 ± 0.5 kDa on SDS-PAGE. Purified ADI exhibited optimum activity at pH 6. Read More

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http://dx.doi.org/10.1007/s13205-020-02212-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7196117PMC

Isolation and functional validation of stress tolerant gene and its comparative physio-biochemical analysis with transgenic tobacco plants overexpressing and .

3 Biotech 2020 May 2;10(5):225. Epub 2020 May 2.

1Molecular Biology and Genetic Engineering Division, Vasantdada Sugar Institute, Manjari (Bk), Tal. Haveli, Pune, Maharashtra 412307 India.

MYB transcription factors are one of the most important mediators for the survival of plants under multiple stress responses. In the present study, , encoding a single R3 repeat MYB DNA binding domain was isolated from stress-tolerant wild relative species of sugarcane . In silico analysis of 948 bp coding mRNA sequence of exhibited the presence of four exons and three introns. Read More

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http://dx.doi.org/10.1007/s13205-020-02197-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7196118PMC

Insights on comparative bacterial diversity between different arid zones of Cholistan Desert, Pakistan.

3 Biotech 2020 May 2;10(5):224. Epub 2020 May 2.

1State Key Laboratory of Biocontrol and Guandong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275 People's Republic of China.

The present study was conducted to analyze bacterial diversity profile of Cholistan desert located in Pakistan. The study investigates the influence of physicochemical parameters of soil on distribution of different bacteria at all taxonomic levels and also study the distribution pattern between different desert environments, particularly rhizospheric and bulk desert sands. Species richness showed phyla and as the dominant OTUs in all the samples. Read More

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http://dx.doi.org/10.1007/s13205-020-02204-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7196105PMC

Endophytic fungi isolated from Brazilian medicinal plants as potential producers of antioxidants and their relations with anti-inflammatory activity.

3 Biotech 2020 May 30;10(5):223. Epub 2020 Apr 30.

Laboratory of Environmental Microbiology, Department of Biology, Federal University of São Carlos, Rodovia João Leme dos Santos Km 110, Sorocaba, SP 18052-780 Brazil.

In this study, 19 endophytic fungi were isolated from and Seventeen strains were molecularly identified as belonging to the genera , and . Assays for detecting antioxidant activity were performed by free radical scavenging activity using the DDPH and ABTS + methods. Based on the results with DPPH, two strains were selected to evaluate the presence of flavonoids and anti-inflammatory activity. Read More

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http://dx.doi.org/10.1007/s13205-020-02211-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193001PMC

Analysis of differentially expressed genes and pathways associated with male sterility lines in watermelon via bulked segregant RNA-seq.

3 Biotech 2020 May 29;10(5):222. Epub 2020 Apr 29.

1State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, 712100 China.

Genic male sterility (GMS) is a common and important trait, which is widely used for the production of hybrid seeds. However, the molecular mechanism of GMS in watermelon remains poorly understood. In this study, we comparatively analyzed the transcriptome profiles of sterile and fertile floral buds using the bulked segregant analysis (BSA) and transcriptome sequencing (RNA-seq). Read More

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http://dx.doi.org/10.1007/s13205-020-02208-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7190782PMC

Application of microbial extracellular carbohydrate polymeric substances in food and allied industries.

3 Biotech 2020 May 28;10(5):221. Epub 2020 Apr 28.

3DBT-Institute of Bioresources and Sustainable Development, Imphal, Manipur 795001 India.

Extracellular polymeric substances (EPS) are biopolymers, composed of polysaccharides, nucleic acids, proteins and lipids, which possess unique functional properties. Despite significant strides made in chemical production processes for polymers, the niche occupied by exopolysaccharides produced by bacteria, yeast or algae is steadily growing in its importance. With the availability of modern tools, a lot of information has been generated on the physico-chemical and biological properties using spectrometric tools, while advanced microscopic techniques have provided valuable insights into the structural-functional aspects. Read More

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http://dx.doi.org/10.1007/s13205-020-02200-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7188750PMC

Bridging genomics and field research: draft genome sequence of CR71, an endophytic bacterium that promotes plant growth and fruit yield in L.

3 Biotech 2020 May 28;10(5):220. Epub 2020 Apr 28.

1Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Ciudad Universitaria, 58030 Morelia, Michoacán México.

Here we report the draft genome sequence of bacterial strain CR71, consisting of a single chromosome with 5,914,775 base pairs (bp), 34.7% G + C content, and 5733 protein-coding genes. Phylogenetic analysis indicates that the CR71 strain is affiliated with species, with an average nucleotide identity > 96% and genome to genome distance > 70%. Read More

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http://dx.doi.org/10.1007/s13205-020-02209-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7188745PMC

Plant G-protein signaling cascade and host defense.

3 Biotech 2020 May 28;10(5):219. Epub 2020 Apr 28.

3Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India.

The heterotrimeric guanine-nucleotide-binding proteins (G-proteins) play a crucial role in signal transduction and regulate plant responses against biotic and abiotic stresses. Necrotrophic pathogens trigger Gα subunit and, in contrast, sometimes Gβγ dimers. Beneficial microbes play a vital role in the activation of heterotrimeric G-proteins in plants against biotrophic and necrotrophic pathogens. Read More

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http://dx.doi.org/10.1007/s13205-020-02201-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7188744PMC

Suppression of -glycan processing enzymes by deoxynojirimycin in tomato () fruit.

3 Biotech 2020 May 28;10(5):218. Epub 2020 Apr 28.

1Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysuru, 570 020 India.

The present study investigated the potential of a small molecule inhibitor, 1-deoxynojirimycin (DNJ), to extend the shelf life of tomatoes. The optimum concentration of DNJ and the proper ripening stage for treatment were standardized using response surface methodology, following a central composite design. The concentration of DNJ used for the analysis was 0. Read More

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http://dx.doi.org/10.1007/s13205-020-02196-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7186287PMC

Improved production of the recombinant phospholipase A1 from wasp venom expressed in bacterial cells for use in routine diagnostics.

3 Biotech 2020 May 27;10(5):217. Epub 2020 Apr 27.

4Arthropod Molecular Biology Laboratory-LBMA-IB, University of Sao Paulo State (UNESP/RC), Ave. 24-A, 1515- Bela Vista, Rio Claro, SP 13506-900 Brazil.

Phospholipase A1 (PLA1) is one of the three major allergens identified in the venom of (Hymenoptera: Vespidae), a clinically relevant wasp from southeastern Brazil. The recombinant form of this allergen (rPoly p 1) could be used for the development of molecular diagnostic of venom allergy. Early attempts to produce rPoly p 1 using BL21 (DE3) cells rendered high yields of the insoluble rPoly p 1 but with low levels of solubilized protein recovery (12%). Read More

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http://dx.doi.org/10.1007/s13205-020-02202-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7186289PMC

Optimization of fermentation conditions for the production of recombinant feruloyl esterase from B1213.

3 Biotech 2020 May 27;10(5):216. Epub 2020 Apr 27.

1Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), No 11 Fucheng Street, Haidian District, Beijing, 100048 China.

Statistical experimental designs were used to optimize conditions for recombinant feruloyl esterase (BpFae) production in bacteria under lactose induction. After optimization by single factor design, Plackett-Burman design, steepest ascent design and the response surface method, the optimal conditions for BpFae production were: 6 g/L lactose, pH 5.5, pre-induced period 5 h, 23 °C, shaker rotational speed of 240 rpm, medium volume of 50 mL/250 mL, inoculum size 0. Read More

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http://dx.doi.org/10.1007/s13205-020-02198-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7186284PMC

Optimization of activin-A: a breakthrough in differentiation of human induced pluripotent stem cell into definitive endoderm.

3 Biotech 2020 May 27;10(5):215. Epub 2020 Apr 27.

6Department of Translational Research and Cellular Therapeutics, Diabetes and Metabolism Research Institute, Beckman Research Institute of City of Hope, Duarte, USA.

The first step in differentiation of pluripotent stem cell toward endoderm-derived cell/organ is differentiation to definitive endoderm (DE) which is the central issue in developmental biology. Based on several evidences, we hypothesized that activin-A optimization as well as replacement of fetal bovine serum (FBS) with knockout serum replacement (KSR) is important for differentiation of induced pluripotent stem cell (iPSC) line into DE. Therefore, a stepwise differentiation protocol was applied on R1-hiPSC1 cell line. Read More

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http://dx.doi.org/10.1007/s13205-020-02215-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7186283PMC

Decolorization and biodegradation of textile di-azo dye Acid Blue 113 by AK6.

3 Biotech 2020 May 25;10(5):214. Epub 2020 Apr 25.

UGC-CAS Department of Biosciences, Saurashtra University, Rajkot, Gujarat 360005 India.

Textile industry is one of the anthropogenic activities that consume a large amount of water and pollute water bodies. It uses a massive amount of dyes, which is one of the main constituents of polluting textile effluent. In the present research, biodegradation of Acid Blue 113 dye, a commonly used textile di-azo dye, has been studied exploiting , strain AK6. Read More

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http://dx.doi.org/10.1007/s13205-020-02205-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182651PMC

Development of a visual detection method for by reverse transcription loop-mediated isothermal amplification.

3 Biotech 2020 May 24;10(5):213. Epub 2020 Apr 24.

1ICAR-Central Potato Research Institute, Shimla, HP 171 001 India.

A reverse transcription-loop mediated isothermal amplification (RT-LAMP) assay was developed to detect the (PVS) in potato. Two sets of six novel primers that recognize the coat protein gene sequence of the PVS were designed and RT-LAMP assay was optimized for the parameters such as different concentrations of primers, MgSO, betaine, dNTPs, DNA polymerase, temperature and duration. The RT-LAMP was carried out under isothermal conditions without the thermal cycler using PVS infected leaf and tuber samples, LAMP specific primers with amplification at 65 °C for 60 min, and 80 °C for 5 min. Read More

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http://dx.doi.org/10.1007/s13205-020-02214-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182647PMC

Biodiscovery of antibacterial constituents from the endolichenic fungi isolated from .

3 Biotech 2020 May 24;10(5):212. Epub 2020 Apr 24.

1The Graduate School, University of Santo Tomas, Manila, Philippines.

A total of nine endolichenic fungi were isolated from the foliose lichen (Nyl.) Hale. Of the nine endolichenic fungi, three taxa (, , ) showed antibacterial activities as determined by the disk diffusion assay against ESKAPE bacterial pathogens. Read More

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http://dx.doi.org/10.1007/s13205-020-02213-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182646PMC

A review on myricetin as a potential therapeutic candidate for cancer prevention.

3 Biotech 2020 May 24;10(5):211. Epub 2020 Apr 24.

School of Life Sciences, Manipal Academy of Higher Education, P.O. Box 345050, Dubai, United Arab Emirates.

Myricetin, one of the most extensively studied polyphenols, is present abundantly in various fruits and vegetables and exhibits diverse pharmacological properties. The multifaceted biological action of myricetin against tumor heterogeneity makes it an impressive anticancer agent whose efficacy has been confirmed by an overwhelming number of studies. Myricetin shows its therapeutic potential by targeting and modulating the expression of various molecular target which are involved in inflammation, cell proliferation, apoptosis, angiogenesis, invasion, and metastasis. Read More

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http://dx.doi.org/10.1007/s13205-020-02207-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181463PMC

Biopesticides in India: technology and sustainability linkages.

3 Biotech 2020 May 24;10(5):210. Epub 2020 Apr 24.

Department of Environmental Science, School for Environmental Sciences, Babasaheb Bhimrao Ambedkar (Central) University, Lucknow, 226025 India.

Despite enhancing the crop yields, the so called green revolution (GR) has proven unsuccessful in assuring long term agricultural sustainability. The methods used for productivity enhancement during GR have not only proven to be problematic but have also resulted in deterioration of soil quality and several other issues related to ecosystems and health issues. The damage was mainly caused by the indiscriminate use of chemical fertilizers and insecticides. Read More

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http://dx.doi.org/10.1007/s13205-020-02192-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181464PMC

Heterologous expression of an SnRK2 protein kinase gene () increases freezing tolerance in transgenic yeast and tobacco.

3 Biotech 2020 May 23;10(5):209. Epub 2020 Apr 23.

1College of Agriculture, Inner Mongolia University for Nationalities, Tongliao, 028042 China.

The sucrose non-fermenting-1 related protein kinase 2 (SnRK2) family plays an important role in the response to abiotic stress. To characterize the function of the gene from in stress protection, we cloned the complete coding sequence of the gene from and generated overexpressing tobacco lines. The open reading frame of was 1083 bp in length and encoded a polypeptide of 360 amino acid residues. Read More

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http://dx.doi.org/10.1007/s13205-020-02203-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181469PMC