2,924 results match your criteria 3 Biotech[Journal]


Characterization of an exopolysaccharide produced by sp. YU16-RN5 and its potential to alleviate cadmium induced cytotoxicity in vitro.

3 Biotech 2021 Dec 9;11(12):491. Epub 2021 Nov 9.

Yenepoya Research Centre, Yenepoya (Deemed to be University), University Road, Deralakatte, Mangalore, 575018 India.

Natural biopolymers have gained remarkable attention for bioremediation particularly in heavy metal removal and oil degradation due to their non-toxic nature and lack of secondary pollution. The exopolysaccharides (EPS) produced by the bacteria have become an important class of biopolymers that are employed in bioremediation. The bacteria isolated from the rhizospheric soil have higher metal tolerance and their EPS are effective in biosorption of heavy metals. Read More

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December 2021

Development of nanobiomaterial for wound healing based on silver nanoparticles loaded on chitosan hydrogel.

3 Biotech 2021 Dec 8;11(12):490. Epub 2021 Nov 8.

Research Department of Zoology, Queen Mary's College (Autonomous), Chennai, Tamil Nadu 600004 India.

The objective of this study was to develop nanobiomaterial containing silver nanoparticles (AgNPs) for wound healing. AgNPs were synthesized using (Sl) aqueous root extract as reducing agent and were characterized physico-chemically using UV-vis spectral studies, XRD, FESEM, TEM, FTIR spectral analysis, DLS, and TG-DSC. Sl AgNPs production was optimized using response surface methodology. Read More

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December 2021

Whole-genome sequence and broad-spectrum antibacterial activity of strain MW-6 isolated from the Arabian Sea.

3 Biotech 2021 Dec 7;11(12):489. Epub 2021 Nov 7.

Department of Industrial Biotechnology, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Islamabad, H-12 Pakistan.

In the current study, strain MW-6 isolated from Arabian seawater exhibited broad-spectrum antibacterial activity against indicator bacterial pathogens. The partially extracted antibacterial metabolites with ethyl acetate revealed promising activity against , and . The minimum inhibitory concentrations (MICs) were determined against indicator strains that ranged from 65-90 µg/ml. Read More

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December 2021

The draft genome sequence of strain DSM 27193, a yeast capable of producing palmitoleic acid-rich lipids.

3 Biotech 2021 Nov 3;11(11):488. Epub 2021 Nov 3.

Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology, Fritz-Haber-Weg4, 76131 Karlsruhe, Germany.

In this study, the draft genome of the recently isolated oleaginous yeast DSM 27193, which can accumulate high content of palmitoleic acid (POA), was sequenced and analyzed. Only few studies have reported about POA-rich lipid production by so far. The ITS region analysis indicated that strain DSM 27193 is closely related to and . Read More

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November 2021

Thermostable and organic solvent-tolerant acid pectinase from FP6: purification, characterization and evaluation of its phytopigment extraction potential.

3 Biotech 2021 Nov 2;11(11):487. Epub 2021 Nov 2.

Department of Microbiology, School of Sciences, JAIN (Deemed-to-be University), 34, 1st Cross, J.C. Road, Bangalore, 560027 Karnataka India.

The present study discusses the purification, characterization and application of pectinase from FP6 in fruit pigment extraction. By the four-step purification involving precipitation, dialysis, ion-exchange chromatography, gel filtration chromatography, a 20.85-fold purification of the enzyme to homogeneity was achieved. Read More

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November 2021

Reduction in immune cell number and loss of 5hmC are associated with lesion grade in cervical carcinogenesis.

3 Biotech 2021 Nov 1;11(11):486. Epub 2021 Nov 1.

Department of Pathology, School of Basic Medical Sciences, Fudan University, 138 Yi Xue Yuan Road, Shanghai, 200032 People's Republic of China.

Tumor genome methylation is closely related to tumor immunosuppression. In the present study, we evaluated the fluctuations in DNA methylation levels, and the numbers of infiltrating T cells and their cytokines in different-grade cervical lesions. A total of 154 human cervical specimens that included LSIL (43 cases), HSIL (48 cases), and cervical squamous cancer (63 cases) were used for this study. Read More

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November 2021

Cuminaldehyde exhibits potential antibiofilm activity against involving reactive oxygen species (ROS) accumulation: a way forward towards sustainable biofilm management.

3 Biotech 2021 Nov 31;11(11):485. Epub 2021 Oct 31.

Department of Biotechnology, The Neotia University, Sarisha, West Bengal 743368 India.

often causes various acute and chronic infections in humans exploiting biofilm. Molecules interfering with microbial biofilm formation could be explored for the sustainable management of infections linked to biofilm. Towards this direction, the antimicrobial and antibiofilm activity of cuminaldehyde, an active ingredient of the essential oil of was tested against . Read More

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November 2021

Improved recognition of splice sites in by incorporating secondary structure information into sequence-derived features: a computational study.

3 Biotech 2021 Nov 31;11(11):484. Epub 2021 Oct 31.

ICAR-Indian Agricultural Statistics Research Institute, New Delhi, 110012 India.

Identification of splice sites is an important aspect with regard to the prediction of gene structure. In most of the existing splice site prediction studies, machine learning algorithms coupled with sequence-derived features have been successfully employed for splice site recognition. However, the splice site identification by incorporating the secondary structure information is lacking, particularly in plant species. Read More

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November 2021

A review on enzymes and pathways for manufacturing polyhydroxybutyrate from lignocellulosic materials.

3 Biotech 2021 Nov 30;11(11):483. Epub 2021 Oct 30.

Institute of Chemistry, Bioscience and Environmental Engineering, Faculty of Science and Technology, University of Stavanger, Stavanger, Norway.

Currently, major focus in the biopolymer field is being drawn on the exploitation of plant-based resources grounded on holistic sustainability trends to produce novel, affordable, biocompatible and environmentally safe polyhydroxyalkanoate biopolymers. The global PHA market, estimated at USD 62 Million in 2020, is predicted to grow by 11.2 and 14. Read More

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November 2021

Correction to: Whole genome sequences of two clinical isolates from India emerging as threats during therapeutic treatment of dermatophytosis.

3 Biotech 2021 Nov 30;11(11):482. Epub 2021 Oct 30.

CSIR-Institute of Genomics and Integrative Biology, New Delhi, 110025 India.

[This corrects the article DOI: 10.1007/s13205-021-02950-1.]. Read More

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November 2021

An encapsulated report on enzyme-assisted transesterification with an allusion to lipase.

3 Biotech 2021 Nov 30;11(11):481. Epub 2021 Oct 30.

Department of Life Sciences, Central University of Tamil Nadu, Neelakudi, Thiruvarur, Tamil Nadu 610 005 India.

Biodiesel is a renewable, sulfur-free, toxic-free, and low carbon fuel which possesses enhanced lubricity. Transesterification is the easiest method employed for the production of biodiesel, in which the oil is transformed into biodiesel. Biocatalyst-mediated transesterification is more advantageous than chemical process because of its non-toxic nature, the requirement of mild reaction conditions, absence of saponification, easy product recovery, and production of high-quality biodiesel. Read More

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November 2021

Nanomaterial conjugated lignocellulosic waste: cost-effective production of sustainable bioenergy using enzymes.

3 Biotech 2021 Nov 30;11(11):480. Epub 2021 Oct 30.

Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Solan, Himachal Pradesh India.

The demand for novel and renewable sources of energy has increased as a result of rapid population growth, limited sources of bioenergy, and environmental pollution, caused by excessive use of fossil fuels. The need to meet future energy demands have motivated researchers to search for alternative and sustainable sources of energy. The bioconversion of lignocellulosic waste (agricultural and food waste) into biofuels shows competitive promises. Read More

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November 2021

Biotechnological potential of psychrophilic microorganisms as the source of cold-active enzymes in food processing applications.

3 Biotech 2021 Nov 30;11(11):479. Epub 2021 Oct 30.

Institute of Bioresources and Sustainable Development, Regional Centre, Sikkim, India.

Microorganisms striving in extreme environments and exhibiting optimal growth and reproduction at low temperatures, otherwise known as psychrophilic microorganisms, are potential sources of cold-active enzymes. Owing to higher stability and cold activity, these enzymes are gaining enormous attention in numerous industrial bioprocesses. Applications of several cold-active enzymes have been established in the food industry, e. Read More

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November 2021

An overview of the phytosynthesis of various metal nanoparticles.

3 Biotech 2021 Nov 29;11(11):478. Epub 2021 Oct 29.

Amity Institute of Microbial Technology, Amity University Rajasthan, Kant-Kalwar, Jaipur, Rajasthan India.

Nanotechnology is an emerging branch of science wherein various valuable molecules with altered properties can be synthesized and utilized for numerous technological applications. Nowadays, nanotechnology is the preferred tool for the agriculture, food, and medicine industries. However, consistent accumulation of toxic by-products during the synthesis of nanoparticles from the established physical and chemical methods imposes an unprecedented danger to the environment and human well-being. Read More

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November 2021

Biosynthesis of l-phosphinothricin with enzymes from chromosomal integrated expression in .

3 Biotech 2021 Nov 28;11(11):477. Epub 2021 Oct 28.

Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014 China.

Phosphinothricin (PPT) is one of the most prevalently using herbicides. The commercial phosphinothricin products are generally in the form of a racemic mixture, of which only the l-phosphinothricin (L-PPT) gives herbicidal function. Synthesis of optically pure L-PPT by deracemization of D/L-PPT is a promising way to cut down the environmental burden and manufacturing cost. Read More

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November 2021

Differential DNA methylation between long-winged and short-winged adults of .

3 Biotech 2021 Nov 26;11(11):476. Epub 2021 Oct 26.

State Key Laboratory for Biocontrol and Institute of Entomology, Sun Yat-sen University, Guangzhou, 510275 China.

a catastrophic rice pest in South East Asia, has adults with wing dimorphism. DNA methylation has been proved to play an important role in regulation of phenotype differentiation in insects. In this study, methylation sensitive amplification polymorphism (MSAP) was used to investigate the cytosine methylation state at CCGG sites in macropterous male adults (MMA) and brachypterous male adults (BMA) of brown planthopper. Read More

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November 2021

Trends in biological data integration for the selection of enzymes and transcription factors related to cellulose and hemicellulose degradation in fungi.

3 Biotech 2021 Nov 26;11(11):475. Epub 2021 Oct 26.

Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, SP Brazil.

Fungi are key players in biotechnological applications. Although several studies focusing on fungal diversity and genetics have been performed, many details of fungal biology remain unknown, including how cellulolytic enzymes are modulated within these organisms to allow changes in main plant cell wall compounds, cellulose and hemicellulose, and subsequent biomass conversion. With the advent and consolidation of DNA/RNA sequencing technology, different types of information can be generated at the genomic, structural and functional levels, including the gene expression profiles and regulatory mechanisms of these organisms, during degradation-induced conditions. Read More

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November 2021

Development and validation of the gene-based functional marker to assist fruit shape selection in tomato.

3 Biotech 2021 Nov 25;11(11):474. Epub 2021 Oct 25.

Department of Plant Breeding and Genetics, Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur, Bihar 813210 India.

Fruit size, shape and colour are important determinants of fruit quality in tomato. Among the different genetic factors, the gene is a key regulator of fruit elongation in tomato. The loss-of-function recessive allele results from a functional single nucleotide polymorphism (SNP) in the second exon of the gene to produce fruit elongation and variable fruit shapes in different genetic backgrounds. Read More

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November 2021

Revealing the potential of PVN-1 for plant beneficial attributes by genome sequencing and analysis.

3 Biotech 2021 Nov 25;11(11):473. Epub 2021 Oct 25.

Environmental Biotechnology and Genomics Division, CSIR-National Environmental Engineering Research Institute, Nehru Marg, Nagpur, 440020 India.

Genome sequencing of PVN-1, isolated from effluent treatment plant (ETP), generates a 5.064 Mb draft genome with 57.6% GC content. Read More

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November 2021

Restriction-free cloning for molecular manipulation and augmented expression of banana bunchy top viral coat protein.

3 Biotech 2021 Nov 22;11(11):471. Epub 2021 Oct 22.

Division of Plant Pathology, Banana Research Station, Kannara, Kerala Agricultural University, Thrissur, Kerala 680652 India.

(BBTV) causing bunchy top disease, is one of the most devastating diseases of banana and plantain. All the six genomic components of isolates from different parts of the world have been well characterised, with most of the studies focusing on replicase gene and coat protein gene. Overexpression of coat protein (CP) in system can contribute significantly in structural as well as immunological studies. Read More

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November 2021

Establishment of TaqMan-based real-time PCR assay for rapid detection of duck circovirus.

3 Biotech 2021 Nov 22;11(11):470. Epub 2021 Oct 22.

College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036 Anhui Province China.

Duck circovirus (DuCV) is widespread across the world and causes feather disorders in young ducks. It was identified as the causative pathogen of duck beak atrophy and dwarfism syndrome and primary sclerosing cholangitis. In this study, we aimed to establish a TaqMan-based real-time PCR assay to detect DuCV. Read More

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November 2021

Rhizosphere microbial diversity in rhizosphere of intercropped with maize.

3 Biotech 2021 Nov 22;11(11):469. Epub 2021 Oct 22.

College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070 China.

Dry tubers of (Thunb.) Breit are used in traditional Chinese medicine. Commonly known as "banxia" in China, the tubers contain valuable compounds, including alkaloids and polysaccharides that are widely used in pharmaceuticals. Read More

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November 2021

Characterization and evolutionary analysis of Cucumber mosaic virus isolate infecting in India.

3 Biotech 2021 Nov 21;11(11):468. Epub 2021 Oct 21.

Plant Pathology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow, 226015 India.

Cucumber mosaic virus (CMV) is one of the most widespread viruses that infects a large number of cultivated crops worldwide and causes severe losses. Besides vegetables and ornamental crops, it is also spreading on medicinal and aromatic plants (MAPs) at an alarming rate. Natural occurrence of green mosaic, leaf curling, necrosis, and distortion were observed on the leaves of in the experimental field of CSIR-CIMAP, Lucknow, India. Read More

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November 2021

Functional properties and potential application of ethanol tolerant β-glucosidases from and yeasts.

3 Biotech 2021 Nov 21;11(11):467. Epub 2021 Oct 21.

Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista "Júlio de Mesquita Filho", R/Cristóvão Colombo, 2265, Jd Nazareth, Ibilce-Unesp, São José do Rio Preto, São Paulo Brazil.

β-Glucosidases have been extensively investigated to integrate the enzyme complex for cellulose fiber saccharification and for improving the aroma of wine. To produce these enzymes, greater attention has been given to filamentous fungi and bacteria, and few investigations have targeted the potential applications of enzymes secreted by yeasts. Addressing this issue, in this study, β-glucosidases were produced by the and yeasts, via solid state fermentation with wheat bran as a substrate. Read More

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November 2021

Understanding the genomic architecture of clinical mastitis in .

3 Biotech 2021 Nov 20;11(11):466. Epub 2021 Oct 20.

ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana 132001 India.

This study elucidated potential genetic variants and QTLs associated with clinical mastitis incidence traits in breed, Sahiwal. Estimated breeding values for the traits (calculated using Bayesian inference) were used as pseudo-phenotypes for association with genome-wide SNPs and further QTL regions underlying the traits were identified. In all, 25 SNPs were found to be associated with the traits at the genome-wide suggestive threshold ( ≤ 5 × 10) and these SNPs were used to define QTL boundaries based on the linkage disequilibrium structure. Read More

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November 2021

Chemoprophylaxis under sporozoites-lumefantrine (CPS-LMF) immunization induce protective immune responses against sporozoites infection in mice.

3 Biotech 2021 Nov 19;11(11):465. Epub 2021 Oct 19.

Department of Biology, College of Science, University of Ha'il, PO Box 2440, Ha'il, Saudi Arabia.

Malaria represents one of the major life-threatening diseases that poses a huge socio-economic impact, worldwide. Chemoprophylaxis vaccination using a relatively low number of wild-type infectious sporozoites represents an attractive and effective vaccine strategy against malaria. However, the role of immune responses to pre-erythrocytic versus blood-stage parasites in protection against different antimalarial drugs remains unclear. Read More

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November 2021

Modulatory effect of euxanthone in liver cancer-bearing obese mice: crosstalk between PPARγ and TIMP3 signalling axes.

3 Biotech 2021 Nov 19;11(11):464. Epub 2021 Oct 19.

Department of General Surgery, Weinan Central Hospital, Weinan, 714000 Shaanxi China.

Liver cancer is one of the prominent cancer-associated fatal diseases with > 80% of cases befall in low-middle resource nations worldwide. In the current study, we studied the effect of euxanthone (EUX) on obesity-associated liver cancer using a high-fat diet-fed mouse model of diethylnitrosamine (DEN)-provoked hepatocellular carcinoma. Mice with 2 weeks of age were intraperitoneally injected with diethylnitrosamine (DEN) 25 mg/kg b. Read More

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November 2021

Quantification of adulteration in traded ayurvedic raw drugs employing machine learning approaches with DNA barcode database.

3 Biotech 2021 Nov 18;11(11):463. Epub 2021 Oct 18.

Forestry and Human Dimensions Division, Kerala Forest Research Institute, Peechi, Thrissur, 680653 Kerala India.

Adulteration of expensive raw drugs with inferior taxa has become a routine practice, conceding the quality and safety of derived herbal products. In this regard, the study addresses the development of an integrated approach encompassing DNA barcode and HPTLC fingerprinting to authenticate chiefly traded ayurvedic raw drugs in south India [viz. (Roxb. Read More

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November 2021

One-pot microbial bioconversion of wheat bran ferulic acid to biovanillin.

3 Biotech 2021 Nov 18;11(11):462. Epub 2021 Oct 18.

Division of Microbiology, ICAR-Indian Agriculture Research Institute, New Delhi, Delhi 110012 India.

Due to growing consumer preference towards natural ingredients in food products, the production of flavors by microbial biotransformation of agrowastes provides an eco-friendly, cost-effective and sustainable pathway for biovanillin production. In the present study, biovanillin was produced by microbial biotransformation of ferulic acid (FA) using sp. ssr-198. Read More

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November 2021

Establishment of hydrolysis probe system real-time PCR assay for rapid detection of canine circovirus.

3 Biotech 2021 Nov 23;11(11):472. Epub 2021 Oct 23.

Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036 People's Republic of China.

In the study, we established a hydrolysis probe-based real-time polymerase chain reaction (PCR) assay to rapidly detect Canine circovirus (CanineCV) DNA in faecal samples. We designed a pair of specific primers and one probe targeting in CanineCV, and sensitivity, specificity, and repeatability tests were performed to evaluate the efficacy of the assay. The assay showed high sensitivity and a minimum detection limit of 8. Read More

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November 2021