1,603 results match your criteria 3 Biotech[Journal]


Differential expression of Helios, Neuropilin-1 and FoxP3 in head and neck squamous cell carcinoma (HNSCC) patients.

3 Biotech 2019 May 16;9(5):178. Epub 2019 Apr 16.

School of Life Sciences, B S Abdur Rahman Crescent Institute of Science and Technology, GST Road, Vandalur, Chennai, Tamil Nadu 600048 India.

In recent years, studies have begun to explore the immune involvement in head and neck tumors. Advanced stage head and neck squamous cell carcinoma (HNSCC) has a poor prognosis with low survival rates with high level of immune infiltrates. Tregs (regulatory T cells) play a crucial role in constructing an immunosuppressive tumor microenvironment. Read More

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http://dx.doi.org/10.1007/s13205-019-1707-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468022PMC

Development and utilization of A and B gene-based diagnostics for the phytoplasma classified under 16Sr I group in plants and insects.

3 Biotech 2019 May 13;9(5):177. Epub 2019 Apr 13.

2Amity Institute of Biotechnology, Amity University, Manesar, Haryana 122413 India.

In the present study, a new set of primers of A and B genes of the phytoplasma genome were designed and validated for the successful detection and taxonomic classification of the previously identified phytoplasma strains of ' P. asteris' (16SrI-B subgroup) associated with Catharanthus leaf yellows, sesame phyllody and the leafhopper (). Our results suggested the ability and sensitivity of and genes as an alternative molecular marker to identify the . Read More

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http://link.springer.com/10.1007/s13205-019-1706-8
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http://dx.doi.org/10.1007/s13205-019-1706-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6462283PMC
May 2019
1 Read

Matrix attachment regions as a tool to influence plant transgene expression.

3 Biotech 2019 May 12;9(5):176. Epub 2019 Apr 12.

Biotron, Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Puschino, Russia.

The inclusion of special regulatory sequences known as matrix attachment regions (MARs) in transgene constructs has been suggested as a possible approach to stabilise the expression of foreign heterological genes. The present review provides a brief summary regarding the MARs that have been used in investigations studying their influence on plant transgene expression in different plants with different promoters and reporter genes, and the comparison of these investigations. Read More

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http://dx.doi.org/10.1007/s13205-019-1709-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461602PMC

Elevated temperature modulates rice pollen structure: a study from foothill of Himalayan agro-ecosystem in India.

3 Biotech 2019 May 11;9(5):175. Epub 2019 Apr 11.

3Forest Ecology and Climate Change Division, Forest Research Institute, Dehradun, Uttarakhand India.

Rice cultivation is practiced in various agro-climatic zones ranging from tropical to temperate. Anthesis is a critical stage for deciding productivity which is controlled by numerous biotic and abiotic stresses. Elevated temperature is one of the key abiotic stresses that adversely modulate the pollen formation and structure, i. Read More

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http://dx.doi.org/10.1007/s13205-019-1700-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459444PMC

Exploring the microbiota and metabolites of traditional rice beer varieties of Assam and their functionalities.

3 Biotech 2019 May 10;9(5):174. Epub 2019 Apr 10.

1Molecular Biology and Microbial Biotechnology Laboratory, Division of Life Sciences, Institute of Advanced Study in Science and Technology (IASST), Guwahati, Assam 781035 India.

Rice beer is traditionally prepared and consumed by various ethnic populations in the Southeast Asian countries. To understand the probable effects of rice beer on human health, present research was aimed to study biochemical parameters, microbial diversity and metabolites of major rice beer varieties of Assam, namely ( and ), and Alcoholic content of rice beer varieties varied from 9.41 to 19. Read More

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http://dx.doi.org/10.1007/s13205-019-1702-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458194PMC

Assessing oil accumulation in the oleaginous yeast JRC1 using dairy waste cheese whey as a substrate.

3 Biotech 2019 May 10;9(5):173. Epub 2019 Apr 10.

Environmental Biotechnology Laboratory, Department of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur (IIT J), Jodhpur, Rajasthan 342037 India.

This study assesses the potential for the lipid production by the oleaginous yeast JRC1 using dairy industry waste cheese whey as a substrate. Cheese whey was used either untreated (UCW) or deproteinized (DCW) at different concentrations (25-100%) to serve as the carbon and energy source. Both UCW and DCW supported high biomass and lipid productivities. Read More

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http://dx.doi.org/10.1007/s13205-019-1701-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458212PMC

Tunicates as a biocontrol tool for larvicides acute toxicity of Zika virus vector .

3 Biotech 2019 May 9;9(5):172. Epub 2019 Apr 9.

4Department of Zoology, Ryan Institute, School of Natural Science, National University of Ireland, Galway, Ireland.

In this present study, we conducted untargeted metabolic profiling using gas chromatography-mass spectrometry (GC-MS) analysis of ascidian to assess the chemical constituents by searching in NIST library with promising biological properties against anti-bacterial and Zika virus vector mosquitocidal properties. Metabolites, steroids and fatty acids are abundant in crude compounds of ascidian and showed potential zone growth inhibition against bacterial strains (10 mm). The active crude compounds of exhibited prominent larvicidal activity against the Zika vector mosquitoes of (LC values of 0. Read More

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http://dx.doi.org/10.1007/s13205-019-1699-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456632PMC

Biochemical characterization of a key laccase-like multicopper oxidase of artificially cultivable provides insights into plant-litter decomposition.

3 Biotech 2019 May 9;9(5):171. Epub 2019 Apr 9.

2National-local Joint Engineering Laboratory of Breeding and Cultivation of Edible and Medicinal Fungi, Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China.

The aim of this study is to determine the key laccase-encoding gene in the life cycle of SCYDJ1-A1, and to characterize the biochemical properties of the laccase. Two laccase-like multicopper oxidase (LMCO) genes were identified in the genome of SCYDJ1-A1 as putative laccase-encoding genes. The two genes, belonging to Auxiliary Activity family 1 subfamily 3, were named as MiLacA and MiLacB. Read More

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http://dx.doi.org/10.1007/s13205-019-1688-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456629PMC

A rapid and sensitive enzymatic assay for 2,3-butanediol.

3 Biotech 2019 May 8;9(5):170. Epub 2019 Apr 8.

1Korea Institute of Ocean Science and Technology, Busan, 49111 Republic of Korea.

In this study, we developed a rapid and sensitive enzymatic assay for 2,3-butanediol (2,3-BDO) detection. The concentration of 2,3-BDO was determined by measuring the reduction of NADP using 2,3-butanediol dehydrogenase (CL-Bdh). The enzymatic assay could detect as low as 0. Read More

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http://dx.doi.org/10.1007/s13205-019-1705-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453985PMC

Biochemical characterization of acyl-coenzyme A synthetases involved in mycobacterial steroid side-chain catabolism and molecular design: synthesis of an anti-mycobacterial agent.

3 Biotech 2019 May 8;9(5):169. Epub 2019 Apr 8.

College of Life Sciences, Sichuan Normal University, Chengdu, 610068 People's Republic of China.

The metabolism of host cholesterol by is an important factor for both its virulence and pathogenesis. However, the rationale for this cholesterol metabolism has not been fully understood yet. In the present study, we characterized several previously undescribed acyl-CoA synthetases that are involved in the steroid side-chain degradation in , and an analogue of intermediate from steroid degradation, 5'--(lithocholoyl sulfamoyl) adenosine (LCA-AMS), was successfully designed and synthesized to be used as a specific anti-mycobacterial agent. Read More

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http://link.springer.com/10.1007/s13205-019-1703-y
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http://dx.doi.org/10.1007/s13205-019-1703-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453995PMC
May 2019
1 Read

Evolution of endonuclease IV protein family: an in silico analysis.

3 Biotech 2019 May 6;9(5):168. Epub 2019 Apr 6.

Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, Pilani, Rajasthan 333031 India.

DNA repair is one of the key cellular events which balances between evolvability and integrity of the genome. Endonuclease IV enzymes are class II AP endonucleases under base excision repair pathway which act on abasic site and break the phosphodiester bond at the 5' side. The role and activity of endonuclease IV proteins vary among different organisms; even it is absent in higher eukaryotes. Read More

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http://link.springer.com/10.1007/s13205-019-1696-6
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http://dx.doi.org/10.1007/s13205-019-1696-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6451744PMC
May 2019
1 Read

Taxonomic characterization and antimicrobial compound production from BDK01 isolated from marine sediment.

3 Biotech 2019 May 4;9(5):167. Epub 2019 Apr 4.

4Department of Biotechnology, Bharathiar University, Coimbatore, 641 046 India.

sp. isolated from marine sediment collected from Palk Strait, Bay of Bengal was investigated for its antagonistic potential. The isolate exhibited antimicrobial activity against selected bacterial strains of clinical importance such as MTCC 3160, NCIM 2327, (methicillin resistant), MTCC 1698, (ESBL), MTCC 1457, and Phenotypic and molecular characterization ascertained the isolate BDK01 as . Read More

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http://dx.doi.org/10.1007/s13205-019-1687-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449414PMC

Ectopic expression of GmZAT4, a putative C2H2-type zinc finger protein, enhances PEG and NaCl stress tolerances in .

3 Biotech 2019 May 4;9(5):166. Epub 2019 Apr 4.

1School of Agriculture, Institute of Agricultural Bioengineering, Shanxi Agricultural University, Taigu, 030801 Shanxi People's Republic of China.

The zinc finger protein (ZFP) transcription factor family plays an important role in regulating plant growth, development, and response to abiotic stress. In this study, we aimed to determine the role of , a C2H2-type transcription factor, in abiotic stress tolerance. The complete coding sequence of the gene was isolated from soybean root RNA, which shows highest expression level compared with leaf, flower and other tissues. Read More

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http://dx.doi.org/10.1007/s13205-019-1673-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449412PMC

Identification of new members of alkaliphilic lipases in archaea and metagenome database using reconstruction of ancestral sequences.

3 Biotech 2019 May 4;9(5):165. Epub 2019 Apr 4.

1Department of Microbiology, Maharaja Ganga Singh University, 334004 Bikaner, India.

The application of bioinformatics in lipase research has the potential to discover robust members from different genomic/metagenomic databses. In this study, we explored the diversity and distribution of alkaliphilic lipases in archaea domain and metagenome data sets through phylogenetic survey. Reconstructed ancestral sequence of alkaphilic lipase was used to search the homologous alkaliphilic lipases among the archaea and metagenome public databases. Read More

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http://dx.doi.org/10.1007/s13205-019-1693-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449409PMC

Diversity of fungi from mangrove sediments of Goa, India, obtained by metagenomic analysis using Illumina sequencing.

3 Biotech 2019 May 4;9(5):164. Epub 2019 Apr 4.

Department of Microbiology, Goa University, Taleigao Plateau, Goa 403206 India.

The fungal composition, abundance and diversity of the mangrove sediments from the Mandovi and Zuari estuaries, Goa, using paired-end Illumina sequencing, hitherto unexplored by a metagenomic approach, indicated that though the types of fungal phyla were similar between the two sediments, the abundance of the species was significantly different between them ( value < 0.005). Basidiomycota and Ascomycota were the two major phyla which were sub-divided into eighteen classes, families, orders, genera and species and one unassigned group in both the sediments. Read More

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http://dx.doi.org/10.1007/s13205-019-1698-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449407PMC

Quorum sensing inimical activity of against gram negative bacterial pathogens by signalling interference.

3 Biotech 2019 Apr 1;9(4):163. Epub 2019 Apr 1.

1Bioresource Technology Lab, Department of Biotechnology, Thiruvalluvar University, Vellore, TN India.

Bacterial virulence is controlled by a cascade of genes influenced by quorum sensing alias bacterial signalling.The present study was intended to develop an effective module that could constrain bacterial communication without harming the host. Quorum quenching ability of was screened upon chromogenic reporter strains such as , and . Read More

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http://dx.doi.org/10.1007/s13205-019-1695-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443769PMC

Complete genome sequence of a marine-sediment-derived bacterial strain SH-B74, a cyclic lipopeptides producer and a biopesticide.

3 Biotech 2019 Apr 1;9(4):162. Epub 2019 Apr 1.

2Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016 China.

A marine-sediment sample-derived strain SH-B74 has the capacity to produce cyclic lipopeptides (CLPs), and these CLPs secreted by the strain show biological activities against various pests under both in vitro and in planta conditions, such evidence has supported that the strain SH-B74 is a biopesticide. To get a better insight into the mechanisms on the control of the pesticides by the strain, a genome sequencing project has been applied to the genomic DNA of the strain SH-B74. The results show that the strain SH-B74 has a chromosome size of 4,042,190 bp, with a GC content of 46. Read More

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http://dx.doi.org/10.1007/s13205-019-1694-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443728PMC
April 2019
2 Reads

Transcriptome sequencing of Himalayan Raspberry () and development of simple sequence repeat markers.

3 Biotech 2019 Apr 30;9(4):161. Epub 2019 Mar 30.

4Department of Fruit Science, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan, India.

Key Message: is a wild crop having less number of EST-SSR markers. First attempt was made towards the transcriptomics data analysis and generation of EST-SSR markers which were used in genetic diversity and transferability studies.

Abstract: is a raspberry with yellow fruits, native to tropical and subtropical India and Asia. Read More

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http://link.springer.com/10.1007/s13205-019-1685-9
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http://dx.doi.org/10.1007/s13205-019-1685-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441421PMC
April 2019
1 Read

Molecular characterization of laccase genes from the basidiomycete Bm-2 and analysis of the 5' untranslated region (5'UTR).

3 Biotech 2019 Apr 30;9(4):160. Epub 2019 Mar 30.

4Unidad de Energía Renovable, Centro de Investigación Científica de Yucatán, Carretera Sierra Papacal-Chuburná Puerto Km 5, 97302 Mérida, Yucatán Mexico.

The aim of this study was to identify and characterize laccase genes produced by Bm-2 in a liquid medium, both with and without induction. The amplification of 5'and 3'regions of laccase sequences was obtained by the RACE-PCR method, and these were assembled to obtain a cDNA of total length. Two new laccase genes were isolated from basal medium (-) and lignocellulosic grapefruit substrate (-), both encoding open reading frames of 2566 bp. Read More

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http://dx.doi.org/10.1007/s13205-019-1691-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441420PMC
April 2019
1 Read

Synergistic effect of co-treatment with all- retinoic acid and 9- retinoic acid on human lung cancer cell line at molecular level.

3 Biotech 2019 Apr 30;9(4):159. Epub 2019 Mar 30.

Department of Biotechnology, Karunya Institute of Technology and Sciences, Karunya Nagar, Coimbatore, Tamil Nadu 641 114 India.

The major challenge in treating cancers with ATRA is the limited availability inside the cell and resistance developed in prolonged treatment. We made an attempt for co-treatment of human NSCLC cell lines (A549) with ATRA and its isomeric precursor (9RA). In this study, the growth inhibitory effect of ATRA, 9RA and combination of both were tested in A549 cells by MTT and Trypan blue assays. Read More

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http://dx.doi.org/10.1007/s13205-019-1692-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441431PMC
April 2019
1 Read

Dual-targeting by CRISPR/Cas9 leads to efficient point mutagenesis but only rare targeted deletions in the rice genome.

3 Biotech 2019 Apr 28;9(4):158. Epub 2019 Mar 28.

1Department of Crop, Soil and Environmental Sciences, University of Arkansas, Fayetteville, AR 72701 USA.

The present study investigated the efficiency of CRISPR/Cas9 in creating genomic deletions as the basis of its application in removing selection marker genes or the intergenic regions. Three loci, representing a transgene and two rice genes, were targeted at two sites each, in separate experiments, and the deletion of the defined fragments was investigated by PCR and sequencing. Genomic deletions were found at a low rate among the transformed callus lines that could be isolated, cultured, and regenerated into plants harboring the deletion. Read More

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http://dx.doi.org/10.1007/s13205-019-1690-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439133PMC
April 2019
2 Reads

Comparative genomics of 40 edible and medicinal mushrooms provide an insight into the evolution of lignocellulose decomposition mechanisms.

3 Biotech 2019 Apr 28;9(4):157. Epub 2019 Mar 28.

1Institute of Microbiology, Beijing Forestry University, Beijing, 100083 People's Republic of China.

Diversity comparison and phylogenetic analyses of carbohydrate-active enzymes (CAZymes), auxiliary activities (AAs) and cytochromes P450 among 40 fungi, which are based on different nutritional pathways, help clarify and explain their divergence and improvement of various life-styles. Molecular clock analyses allow us to understand the evolutionary and developmental rules in decomposition gene families. Our results suggested that fungi in different ecological types acquired an obvious preference on specific decomposing gene families during evolutionary selection. Read More

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http://dx.doi.org/10.1007/s13205-019-1689-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439079PMC
April 2019
1 Read

Comparative study of different molecular weight pullulan productions by CGMCC No.11602.

3 Biotech 2019 Apr 28;9(4):156. Epub 2019 Mar 28.

1Shaanxi Provincial Institute of Microbiology, No. 76 Xiying Rd, Xi'an, 710043 Shaanxi Province People's Republic of China.

Pullulan productions by CGMCC No.11602 were conducted using the initial culture (IC) medium and the optimized culture (OC) medium, respectively, in which pullulan with significantly different molecular weights was obtained. Under the IC medium condition, the pullulan molecular weight () reached 288,403 Da with a yield of 64. Read More

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http://dx.doi.org/10.1007/s13205-019-1680-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439089PMC
April 2019
1 Read

Biodegradation of polycyclic aromatic hydrocarbons (PAHs) in a diesel oil-contaminated mangrove by plant growth-promoting rhizobacteria.

3 Biotech 2019 Apr 27;9(4):155. Epub 2019 Mar 27.

1Laboratório de Microbiologia Aplicada e Bioprospecção, Departamento de Biointeração, Instituto de Ciências da Saúde, Universidade Federal da Bahia (UFBA), Campus Canela, Salvador, Bahia 40110-100 Brazil.

In this study, L. mangrove plants and plant growth-promoting bacteria were evaluated for their ability to degrade polycyclic aromatic hydrocarbons in diesel oil-contaminated sediment. The diesel-contaminated soil was sown with plant growth-promoting bacteria in the L. Read More

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http://dx.doi.org/10.1007/s13205-019-1686-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437237PMC
April 2019
1 Read

Elimination of from infected cormels of three cultivars of gladiolus using thermo-, electro- and chemotherapy.

3 Biotech 2019 Apr 26;9(4):154. Epub 2019 Mar 26.

1Plant Molecular Virology Laboratory, CPMB, CSIR-National Botanical Research Institute, Lucknow, U.P. 226001 India.

(BYMV) is a prevalent virus and major threat to gladiolus cultivation the world over. In the gladiolus repository at CSIR-NBRI, Lucknow, several plants (82-88%) of three economically important cultivars were found infected by BYMV showing severe mosaic and stripe symptoms. Affected plants exhibit diminished quality and quantity of florets and corms, thus reducing their value. Read More

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http://link.springer.com/10.1007/s13205-019-1684-x
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http://dx.doi.org/10.1007/s13205-019-1684-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6435773PMC
April 2019
1 Read

Dark fermentation effluent as substrate for hydrogen production from highlighting the performance of different fermentation systems.

3 Biotech 2019 Apr 26;9(4):153. Epub 2019 Mar 26.

School of Chemical Engineering, Federal University of Uberlandia, Av. João Naves de Ávila 2121, Santa Mônica, Uberlândia, MG 38408-144 Brazil.

Hydrogen production by biological route is a potentially sustainable alternative. Nowadays, energy production from sustainable sources has become urgent for several countries as well as for international policies. In this perspective, hydrogen has gained substantial global attention as clean, sustainable, and versatile energy carrier. Read More

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http://link.springer.com/10.1007/s13205-019-1676-x
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http://dx.doi.org/10.1007/s13205-019-1676-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6435761PMC
April 2019
2 Reads

Transcriptome-wide analysis of the difference of alternative splicing in susceptible and resistant silkworm strains after BmNPV infection.

3 Biotech 2019 Apr 25;9(4):152. Epub 2019 Mar 25.

1Jiangsu University of Science and Technology, Zhenjiang, 212018 Jiangsu People's Republic of China.

Novel alternative splicing events were identified from BmNPV-susceptible and -resistant silkworm strains after BmNPV infection using high-throughput RNA-sequencing strategy. In total, 12.82 Gb clean RNA-seq data were generated for the two midgut samples from BmNPV-susceptible and -resistant silkworm strains, and 14. Read More

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http://dx.doi.org/10.1007/s13205-019-1669-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434002PMC
April 2019
1 Read

Production and characterization of bioemulsifiers from strains isolated from lipid-rich wastewater.

3 Biotech 2019 Apr 25;9(4):151. Epub 2019 Mar 25.

Discipline of Microbiology, School of Life Sciences, University of KwaZulu-Natal, (Westville Campus), Private Bag X54001, Durban, 4000 South Africa.

In this study, two indigenous bacterial strains (Ab9-ES and Ab33-ES) isolated from lipid-rich wastewater showed potential to produce bioemulsifier in the presence of 2% (v/v) olive oil as a carbon source. These bacterial strains were identified as sp. Ab9-ES and sp. Read More

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http://dx.doi.org/10.1007/s13205-019-1683-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434010PMC

Effect of light/dark cycle on nitrate and phosphate removal from synthetic wastewater based on BG11 medium by sp.

3 Biotech 2019 Apr 25;9(4):150. Epub 2019 Mar 25.

1Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.

In this study, microalgae growth in the synthetic wastewater and their ability to remove nutrients under different light levels was investigated. For this purpose, a comparative study was conducted on freshwater microalgae sp. to evaluate their performance to remove nitrate and phosphate from both slaughterhouse and dairy synthetic treated wastewaters, under different light/dark cycles (12/12, 16/8 and 24/0 h), in Erlenmeyer flasks. Read More

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http://dx.doi.org/10.1007/s13205-019-1679-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434003PMC

Docking analysis of hexanoic acid and quercetin with seven domains of polyketide synthase A provided insight into quercetin-mediated aflatoxin biosynthesis inhibition in .

3 Biotech 2019 Apr 25;9(4):149. Epub 2019 Mar 25.

Genomic laboratory, Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Solan, Himachal Pradesh 173234 India.

Studies on phytochemicals as anti-aflatoxigenic agents have gained importance including quercetin. Thus, to understand the molecular mechanism behind inhibition of aflatoxin biosynthesis by quercetin, interaction study with polyketide synthase A (PksA) of was undertaken. The 3D structure of seven domains of PksA was modeled using SWISS-MODEL server and docking studies were performed by Autodock tools-1. Read More

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http://dx.doi.org/10.1007/s13205-019-1675-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431682PMC
April 2019
1 Read

Beetroot juice alleviates isoproterenol-induced myocardial damage by reducing oxidative stress, inflammation, and apoptosis in rats.

3 Biotech 2019 Apr 23;9(4):147. Epub 2019 Mar 23.

6Department Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, 11451 Saudi Arabia.

Beetroot ( L.) juice (BRJ) is a good source of betalain (betacyanins and betaxanthin) pigments and exhibits antioxidant, anti-inflammatory, and chemo-preventive activities in vitro and in vivo. The current study was performed to determine the cardioprotective effect of BRJ on lipid peroxidation, antioxidant defense, functional impairment, and histopathology in rats with isoproterenol (ISP)-induced myocardial injury. Read More

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http://dx.doi.org/10.1007/s13205-019-1677-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6430824PMC

Effects of corn supplementation on the antioxidant activity, selected minerals, and gene expression of selenoprotein and metallothionein in serum, liver, and kidney of sheep-fed palm kernel cake: urea-treated rice straw diets.

3 Biotech 2019 Apr 22;9(4):146. Epub 2019 Mar 22.

1Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor Malaysia.

This study aimed to determine influence of corn inclusion on glutathion peroxidase (GPx) activity, selected minerals concentration, and gene expression in sheep-fed palm kernel cake (PKC) and urea-treated rice straw. Twenty-seven of Dorper sheep were divided into three groups and fed a basal diet of (20% rice straw and 80% concentrate) with addition of ground corn at either 0% (T1), 5% (T2), or 10% (T3), respectively. After 120 days feeding trial, all animals were slaughtered and tissue samples of kidney, liver, and muscles were taken for enzyme and mineral analyses. Read More

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http://link.springer.com/10.1007/s13205-019-1681-0
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http://dx.doi.org/10.1007/s13205-019-1681-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6430260PMC
April 2019
2 Reads

In vitro growth of L. affected by silver nanoparticles.

3 Biotech 2019 Apr 21;9(4):145. Epub 2019 Mar 21.

3Department of Engineering, Federal University of Lavras, Lavras, MG Brazil.

The effect of silver nanoparticles (AgNPs) on plant cells, since their phytotoxicity potential is not yet fully understood. In this context, the aim of the present study was to elucidate the effects of AgNPs in the in vitro culture of . For this purpose, seeds were grown in MS medium supplemented with different concentrations of AgNPs. Read More

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http://dx.doi.org/10.1007/s13205-019-1674-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6430277PMC
April 2019
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Diversity, community distribution and growth promotion activities of endophytes associated with halophyte Murr.

3 Biotech 2019 Apr 21;9(4):144. Epub 2019 Mar 21.

1Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011 People's Republic of China.

The purpose of this study was to investigate the composition, diversity, distribution, and growth promotion activity of endophytic bacteria isolated from Murr. Consequently, a total of 109 endophytic bacteria affiliated to 3 phyla, 12 orders and 36 genera were isolated using nine different selective media, from which, was the dominant taxon containing seven orders at the phylum level; showed the highest diversity containing 12 genera at the family level. Based on PAST and SPSS analysis, species diversity and abundance were mostly isolated from nutritious soil condition (22 genera) and root tissue (27 genera). Read More

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http://dx.doi.org/10.1007/s13205-019-1678-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6428883PMC
April 2019
2 Reads

Transgenic tomatoes for abiotic stress tolerance: status and way ahead.

3 Biotech 2019 Apr 18;9(4):143. Epub 2019 Mar 18.

4ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, Pune, 410505 India.

Tomato () is one of the most important vegetable crops; its production, productivity and quality are adversely affected by abiotic stresses. Abiotic stresses such as drought, extreme temperature and high salinity affect almost every stage of tomato life cycle. Depending upon the plant stage and duration of the stress, abiotic stress causes about 70% yield loss. Read More

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http://dx.doi.org/10.1007/s13205-019-1665-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423223PMC

Psychrotrophs of the genus with potential to weather potassium aluminosilicate mineral.

3 Biotech 2019 Apr 16;9(4):142. Epub 2019 Mar 16.

1ICAR-National Bureau of Agriculturally Important Microorganisms, Kushmaur, Maunath Bhanjan, Uttar Pradesh 275103 India.

In the present investigation, five strains of obtained from Rohtang Pass, Himachal Pradesh, India along with one strain of (IARI-SL-13) were screened qualitatively and quantitatively for their ability to solubilize K at different temperatures ranging from 5 to 30 °C. All the selected strains do not produce violet colonies, instead produced pink or red coloured colonies. In a plate assay, among all the strains tested, sp. Read More

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http://dx.doi.org/10.1007/s13205-019-1672-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420585PMC
April 2019
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Biodegradation of long chain alkanes in halophilic conditions by sp. strain Est-02 isolated from saline soil.

3 Biotech 2019 Apr 15;9(4):141. Epub 2019 Mar 15.

3School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran.

In this study, through a multistep enrichment and isolation procedure, a halophilic bacterial strain was isolated from unpolluted saline soil, which was able to effectively and preferentially degrade long chain alkanes (especially tetracosane and octacosane). The strain was identified by 16S rRNA gene sequence as an sp. The growth of strain Est-02 was optimized at the presence of tetracosane in different NaCl concentrations, temperatures, and pH. Read More

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http://dx.doi.org/10.1007/s13205-019-1670-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420517PMC
April 2019
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Integrated strategy of temperature shift and mannitol feeding for enhanced production of echinocandin B by CCTCC M2012300.

3 Biotech 2019 Apr 14;9(4):140. Epub 2019 Mar 14.

1Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014 China.

The production of echinocandin B (ECB) by CCTCC M2012300 was improved by integrating the temperature-shift and fed-batch control strategies. The kinetic characteristics of batch cultures were analyzed at different culture temperatures, and then a two-stage temperature control strategy was established. In the first 6 days, the temperature was maintained at 30 °C to obtain the maximal cell growth rate; subsequently, 25 °C was used to gain a high ECB formation rate. Read More

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http://dx.doi.org/10.1007/s13205-019-1668-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419687PMC
April 2019
1 Read

Genetic transformation of mediated by HIV-TAT peptide.

3 Biotech 2019 Apr 13;9(4):139. Epub 2019 Mar 13.

1National Research Centre on Plant Biotechnology, Lal Bahadur Shastri Building, Pusa Campus, New Delhi, 110012 India.

Scientific interest in microalgal species is growing and, genetic transformation has definitely opened more avenues, in the ongoing research on microphytes. In the present study, we have attempted to transform by mobilizing double-stranded linear Transfer DNA (T-DNA) comprised of () gene cassette and II (II) gene cassette non-covalently bound to TAT peptide, into cells treated with Triton X-100. The transformed cells when examined under fluorescent microscope, exhibited green fluorescence in comparison to the untransformed cells. Read More

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http://link.springer.com/10.1007/s13205-019-1671-2
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http://dx.doi.org/10.1007/s13205-019-1671-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419685PMC
April 2019
2 Reads

Distribution of antibiotic resistance and virulence factors among the bacteria isolated from diseased .

3 Biotech 2019 Apr 12;9(4):138. Epub 2019 Mar 12.

1School of Biosciences, Mahatma Gandhi University, PD Hills (PO), Kottayam, 686560 India.

Considering the emerging concern with the antimicrobial resistance (AMR) evolution, the study has been designed to identify the antibiotic resistance and virulence properties of culturable bacteria isolated from the diseased fish . This has resulted in the purification of 18 morphologically distinct bacterial isolates which were identified by both biochemical and molecular methods. Antibiotic resistance analysis showed the resistance of these isolates to multiple antibiotics and remarkable evolution of AMR. Read More

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http://dx.doi.org/10.1007/s13205-019-1654-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419682PMC
April 2019
1 Read

Morpho-taxonomic, genetic, and biochemical characterization of freshwater microalgae as potential biodiesel feedstock.

3 Biotech 2019 Apr 12;9(4):137. Epub 2019 Mar 12.

1Biotechnology Group, Biological Science and Technology Division, CSIR-North East Institute of Science and Technology, Jorhat, Assam 785006 India.

In the present study, seven axenic fresh water microchlorophytes were isolated and identified as (NEIST BT-1), (NEIST BT-2), sp. (NEIST BT-10), S sp. (NEIST BT-A6), (NEIST BT-A1), sp. Read More

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http://dx.doi.org/10.1007/s13205-019-1664-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419688PMC
April 2019
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BaCf3: highly thermostable bacteriocin from BTSS3 antagonistic on food-borne pathogens.

3 Biotech 2019 Apr 11;9(4):136. Epub 2019 Mar 11.

Department of Biotechnology, Cochin University of Science and Technology, Cochin, 682 022 India.

In the present study, we characterized bacteriocin BaCf3, isolated and purified from BTSS3, and demonstrated its inhibitory potential on growth and biofilm formation of certain food spoilage bacteria and pathogens. Purification was by gel filtration chromatography and its molecular weight was 3028.422 Da after MALDI-TOF MS. Read More

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http://dx.doi.org/10.1007/s13205-019-1639-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409291PMC

Catalytic and antibacterial properties of gold nanoparticles synthesized by a green approach for bioremediation applications.

3 Biotech 2019 Apr 9;9(4):135. Epub 2019 Mar 9.

1Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, 76230 Santiago de Querétaro, Querétaro Mexico.

In this work, we are proposing the green synthesis of gold nanoparticles (AuNPs) using aqueous extracts of and evaluating their antibacterial and catalytic properties. Characterization was performed by UV-Vis and FT-IR spectroscopies, X-ray diffraction, and transmission electron microscopy (TEM). Antibacterial activity of AuNPs was analyzed using and and catalytic activity was determined by the degradation of methylene blue and congo red. Read More

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http://dx.doi.org/10.1007/s13205-019-1666-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409132PMC

Phylogenetic diversity of sulfate-reducing bacteria of sediments of Chilika Lake, India, determined through analysis of the dissimilatory sulfite reductase () gene.

3 Biotech 2019 Apr 7;9(4):134. Epub 2019 Mar 7.

2Department of Plant Sciences, School of Life Science, University of Hyderabad, Hyderabad, India.

In this study, the sulfate-reducing bacteria, (SRB) were identified and reported for the first time through analysis of functional gene , from the DNA of sediment samples collected from 10 sites of the Chilika lake. The finding illustrates Forty six Operational Taxonomic Units (OTUs), identified from the DGGE which were obtained from the 10 sediment samples. Of these, 34 OTUs exhibited around 78-96% sequence similarity and 12 OTUs showed 97 to 100% sequence similarity to the gene of reported type strains of SRB. Read More

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http://dx.doi.org/10.1007/s13205-019-1655-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405784PMC

Intervention of molecular breeding in water saving rice production system: aerobic rice.

3 Biotech 2019 Apr 7;9(4):133. Epub 2019 Mar 7.

1Department of Molecular Biology and Biotechnology, Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125004 India.

The aerobic rice system/methods developed to tackle shortage of water, is a sustainable method to enhance rice productivity. Approximately 50% of irrigation water could be saved using this system in contrast to lowland rice cultivation. The crop can be directly seeded or transplanted in dry soil in this system rather than irrigated system of rice production. Read More

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http://link.springer.com/10.1007/s13205-019-1657-0
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http://dx.doi.org/10.1007/s13205-019-1657-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405779PMC
April 2019
2 Reads

Genomic characterization of a potentially novel species producing exopolysaccharide.

3 Biotech 2019 Apr 7;9(4):132. Epub 2019 Mar 7.

1Key Laboratory of Leather Chemistry and Engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu, 610065 People's Republic of China.

Human oral streptococci, particularly the mitis group, often dwell in the upper respiratory tracts, oral mucosa, and tooth surfaces of healthy individuals. In this work, an α-hemolytic strain, designated LQJ-218, was isolated from the human oral cavity and evaluated for its ability to produce exopolysaccharides. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain is a potentially novel species belonging to the mitis group streptococci. Read More

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http://dx.doi.org/10.1007/s13205-019-1652-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405781PMC
April 2019
1 Read

Erythropoietin protects neurons from apoptosis via activating PI3K/AKT and inhibiting Erk1/2 signaling pathway.

3 Biotech 2019 Apr 7;9(4):131. Epub 2019 Mar 7.

Department of Neurology, Guizhou Provincial People's Hospital, 83 Dongshan Road, Guizhou, 550000 China.

The aim of this study was to explore the neuroprotective effect and the underlying mechanism of erythropoietin (EPO) on the cortical neuronal cells insulted with oxygen and glucose deprivation (OGD). Different concentrations of EPO were used to determine the anti-apoptosis effect of EPO. In addition, PI3K inhibitor LY294002 and ERK1/2 inhibitor U0126 were added to explore the underlying mechanism of EPO. Read More

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http://link.springer.com/10.1007/s13205-019-1667-y
Publisher Site
http://dx.doi.org/10.1007/s13205-019-1667-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405787PMC
April 2019
5 Reads

Functional characterization of the rice root Germin-like protein gene-1 (RGLP1) promoter in .

3 Biotech 2019 Apr 7;9(4):130. Epub 2019 Mar 7.

2Department of Botany, Faculty of Biological sciences, Quaid-i-Azam University, Islamabad, 45320 Pakistan.

Germin (GER) and germin-like protein (GLP) genes play a very important role against various stresses. Promoter analysis provide significant insight into gene's function and regulation. Presently, upstream region (1228 bp) of the RGLP1 gene was functionally characterized via heterologous expression. Read More

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http://dx.doi.org/10.1007/s13205-019-1644-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405785PMC

Simultaneous laccase production and transformation of bisphenol-A and triclosan using .

3 Biotech 2019 Apr 6;9(4):129. Epub 2019 Mar 6.

1Enzyme Biotechnology Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana 124001 India.

New age micro-pollutants, bisphenol-A (BPA) and triclosan (TCA), known for their carcinogenic effects in living organisms can effectively be removed from water using laccase from . Laccase was produced from JSRK13 in both submerged and solid-state fermentation (SmF and SSF) conditions. In SmF, JSRK13 gave the maximum production of laccase on the 10th day with an activity of 22 U mL, whereas, in SSF 185 U g of the enzyme was produced on the 17th day. Read More

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http://dx.doi.org/10.1007/s13205-019-1648-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403270PMC
April 2019
1 Read