Biomed Res Int 2014 22;2014:379340. Epub 2014 Apr 22.
Department of Biotechnology and Molecular Medicine, A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, 70210 Kuopio, Finland ; Department of Medicine, University of Eastern Finland, 70120 Kuopio, Finland ; Gene Therapy Unit, Kuopio University Hospital, 70120 Kuopio, Finland.
Rare-cutting endonucleases, such as the I-PpoI, can be used for the induction of double strand breaks (DSBs) in genome editing and targeted integration based on homologous recombination. For therapeutic approaches, the specificity and the pattern of off-target effects are of high importance in these techniques. For its applications, the endonuclease needs to be transported into the target cell nucleus, where the mechanism of transport may affect its function. Read More