58 results match your criteria 20000 wells


High-accuracy quantitative principle of a new compact digital PCR equipment: Lab On An Array.

Genomics Inform 2021 Sep 30;19(3):e34. Epub 2021 Sep 30.

Department of Bioconvergence Engineering, Dankook University, Yongin 16890, Korea.

Digital PCR (dPCR) is the third-generation PCR that enables real-time absolute quantification without reference materials. Recently, global diagnosis companies have developed new dPCR equipment. In line with the development, the Lab On An Array (LOAA) dPCR analyzer (Optolane) was launched last year. Read More

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September 2021

Decommissioning Orphaned and Abandoned Oil and Gas Wells: New Estimates and Cost Drivers.

Environ Sci Technol 2021 08 14;55(15):10224-10230. Epub 2021 Jul 14.

Resources for the Future, 1616 P Street NW, Washington, DC 20036, United States.

Millions of abandoned oil and gas wells are scattered across the United States, causing methane emissions and other environmental hazards. Governments are increasingly interested in decommissioning these wells but want to do so efficiently. However, information on the costs of decommissioning wells is very limited. Read More

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Randomized controlled field trial comparing quarter and cow level selective dry cow treatment using the California Mastitis Test.

J Dairy Sci 2021 Aug 30;104(8):9063-9081. Epub 2021 Apr 30.

Quality Milk Management Services Ltd., Cedar Barn, Easton Hill, Easton, Wells, Somerset, BA5 1DU, United Kingdom; School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, LE12 5RD, United Kingdom.

Selective use of antibiotic dry cow treatment can be implemented at the cow or quarter level, with the latter having the potential to further reduce antibiotic use. Our objective was to compare these 2 approaches in 6 herds in the United Kingdom in which environmental mastitis predominated. Eight hundred seven cows were enrolled and categorized as having a high cell count (n = 401) or low cell count (n = 406) in the last 3 mo of lactation and clinical mastitis history. Read More

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Antileishmanial Activity of New Synthesized Schiff and Mannich (Morpholine) Base Compounds.

Turkiye Parazitol Derg 2020 Dec;44(4):216-220

Giresun University Faculty of Medicine, Department of Medical Microbiology, Giresun, Turkey.

Objective: Leishmaniasis is an important parasitic disease in many countries, including ours. A variety of drugs are currently used for its treatment. However, certain side effects of these drugs, such as teratogenicity, hepatotoxicity and nephrotoxicity, have been reported in some patients. Read More

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December 2020

Systemic inflammatory response to inhaled endotoxin does not correlate with airway response.

Respir Res 2019 Nov 12;20(1):252. Epub 2019 Nov 12.

Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina, 104 Mason Farm Rd #CB7310, Chapel Hill, NC, 27599-7310, USA.

Background: Endotoxin is a component of particulate matter linked to respiratory disease. Our group has shown that experimental endotoxin inhalation challenge reproducibly triggers neutrophilic inflammation in the airways and in peripheral blood. Sputum induction is currently the only available method for assessing airway neutrophilia but is laborious and time-consuming. Read More

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November 2019

Population allocation at the housing unit level: estimates around underground natural gas storage wells in PA, OH, NY, WV, MI, and CA.

Environ Health 2019 07 8;18(1):58. Epub 2019 Jul 8.

Center for Climate, Health and the Global Environment, Harvard T.H. Chan School of Public Health, 401 Park Drive, Landmark Center 4th floor west suite 415E, Boston, MA, 02215, USA.

Background: Spatially accurate population data are critical for determining health impacts from many known risk factors. However, the utility of the increasing spatial resolution of disease mapping and environmental exposures is limited by the lack of receptor population data at similar sub-census block spatial scales.

Methods: Here we apply an innovative method (Population Allocation by Occupied Domicile Estimation - ABODE) to disaggregate U. Read More

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Performance Evaluation of STARPAM Polymer and Application in High Temperature and Salinity Reservoir.

Int J Anal Chem 2018 18;2018:9653953. Epub 2018 Dec 18.

College of Mathematics and Statistics, Northeast Petroleum University, Daqing, Heilongjiang 163318, China.

Based on the properties of high temperature and salinity reservoir, the water-soluble polymer with good heat resistance and salt tolerance can be obtained through copolymerization between 2-acrylamide-2-methyl sulfonate monomer (AMPSN) and acrylamide monomer (AM) in water. The star shaped stable complexes (STARPAM) with the star nucleus of -CD are prepared by living radical polymerization, which can improve the viscosity and change the percolation characteristics of the polymer in porous media. In the article, the performance of the STARPAM (star-shaped polymer) with heat resistance and salt tolerance was evaluated by comparing the viscosification property, heat and salt resistance, calcium and magnesium tolerance, and long-term thermal stability of STARPAM (star-shaped polymer) with those of HPAM (partially hydrolyzed polyacrylamide) and MO-4000 (linear polymer). Read More

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December 2018

Relationship between total dissolved solids and electrical conductivity in Marcellus hydraulic fracturing fluids.

Water Sci Technol 2018 May;77(7-8):1998-2004

Department of Agricultural and Biological Engineering, Penn State University, 111 Research Unit A, University Park, PA 16802, USA E-mail:

The production of hydraulic fracturing fluids (HFFs) in natural gas extraction and their subsequent management results in waste streams highly variable in total dissolved solids (TDS). Because TDS measurement is time-consuming, it is often estimated from electrical conductivity (EC) assuming dissolved solids are predominantly ionic species of low enough concentration to yield a linear TDS-EC relationship: TDS (mg/L) = k × EC (μS/cm) where k is a constant of proportionality. HHFs can have TDS levels from 20,000 to over 300,000 mg/L wherein ion-pair formation and non-ionized solutes invalidate a simple TDS-EC relationship. Read More

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Psychotherapy for Major Depressive Disorder and Generalized Anxiety Disorder: A Health Technology Assessment.

Authors:

Ont Health Technol Assess Ser 2017 13;17(15):1-167. Epub 2017 Nov 13.

Background: Major depressive disorder and generalized anxiety disorder are among the most commonly diagnosed mental illnesses in Canada; both are associated with a high societal and economic burden. Treatment for major depressive disorder and generalized anxiety disorder consists of pharmacological and psychological interventions. Three commonly used psychological interventions are cognitive behavioural therapy (CBT), interpersonal therapy, and supportive therapy. Read More

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Projecting the Water Footprint Associated with Shale Resource Production: Eagle Ford Shale Case Study.

Environ Sci Technol 2017 Dec 30;51(24):14453-14461. Epub 2017 Nov 30.

The Bureau of Economic Geology, Jackson School of Geosciences, The University of Texas at Austin , Austin, Texas 78713-8924, United States.

Production of oil from shale and tight reservoirs accounted for almost 50% of 2016 total U.S. production and is projected to continue growing. Read More

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December 2017

[Development of Hybridoma Cell Lines Secreting Monoclonal Antibodies against ADAMTS13].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2017 Aug;25(4):1123-1129

Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China.

Objective: To establish the hybridoma cell lines secreting anti-ADAMTS13-T7 monoclonal antibodies (MA6) and to construct the MAb directed against different domains of ADAMTS13-T7.

Methods: The trucated type protein ADAMTS13 of eukaryote-expressed recombinant ADAMTS13-T7 was constructed and was purified by using Ni-NTA agarose. Then the purified ADAMTS13 was used to immunize the BALB/c mice; the antiserum titer of ADAMTS13 protein of immunized mice was deteceted by ELISA. Read More

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Evaluation of the antibacterial effects of aqueous and ethanolic leaf extracts of () on Streptococcus mutans and Streptococcus sobrinus.

Electron Physician 2016 Dec 25;8(12):3363-3368. Epub 2016 Dec 25.

Dental Materials Research Center, Department of Pediatrics, Faculty of Dentistry, Babol University of Medical Sciences, Babol, Iran.

Introduction: The plant from the family of has many uses in traditional medicine. The aim of the current study was to determine the effects of the aqueous and ethanolic extracts of on and , which cause tooth decay.

Methods: This 2016 study was performed on standardized strains of PTCC1683 and PTCC1601 and clinical isolates. Read More

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December 2016

Biocompatibility evaluation of orthodontic composite by real-time cell analysis.

Hum Exp Toxicol 2016 Aug 1;35(8):833-8. Epub 2015 Oct 1.

Department of Periodontology, Faculty of Dentistry, Selçuk University, Konya, Turkey.

Introduction: The aim of this study was to evaluate the cytotoxic effects of three different light-cured orthodontic composites.

Material And Methods: Light Bond (Reliance orthodontic products), Grengloo (Ormco corporation), and Kurasper F (Kuraray Europe GmbH) were selected for the experiment. Specimens were prepared according to the manufacturers' instructions, measuring 5 mm in diameter and 2 mm in thickness. Read More

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Magnetic Alignment of Microelements Containing Cultured Neuronal Networks for High-Throughput Screening.

J Biomol Screen 2015 Oct 6;20(9):1091-100. Epub 2015 Aug 6.

UNC/NCSU Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill and Raleigh, NC Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC Carolina Institute for Developmental Disabilities, Carrboro, NC

High-throughput screening (HTS) on neurons presents unique difficulties because they are postmitotic, limited in supply, and challenging to harvest from animals or generate from stem cells. These limitations have hindered neurological drug discovery, leaving an unmet need to develop cost-effective technology for HTS using neurons. Traditional screening methods use up to 20,000 neurons per well in 384-well plates. Read More

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October 2015

Applying the ARRIVE Guidelines to an In Vivo Database.

PLoS Biol 2015 May 20;13(5):e1002151. Epub 2015 May 20.

Mammalian Genetics Unit, Medical Research Council, Harwell, United Kingdom.

The Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines were developed to address the lack of reproducibility in biomedical animal studies and improve the communication of research findings. While intended to guide the preparation of peer-reviewed manuscripts, the principles of transparent reporting are also fundamental for in vivo databases. Here, we describe the benefits and challenges of applying the guidelines for the International Mouse Phenotyping Consortium (IMPC), whose goal is to produce and phenotype 20,000 knockout mouse strains in a reproducible manner across ten research centres. Read More

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A review of potable water accessibility and sustainability issues in developing countries - case study of Uganda.

Rev Environ Health 2014 ;29(4):363-78

Providing sources of sustainable and quality potable water in Uganda is a significant public health issue. This project aimed at identifying and prioritizing possible actions on how sustainable high quality potable water in Uganda's water supply systems could be achieved. In that respect, a review of both the current water supply systems and government programs on drinking water in Uganda was completed. Read More

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Biogeochemistry and community ecology in a spring-fed urban river following a major earthquake.

Environ Pollut 2013 Nov 7;182:190-200. Epub 2013 Aug 7.

Department of Soil & Physical Sciences, Faculty of Agriculture & Life Sciences, P.O. Box 84, Lincoln 7674, New Zealand. Electronic address:

In February 2011 a MW 6.3 earthquake in Christchurch, New Zealand inundated urban waterways with sediment from liquefaction and triggered sewage spills. The impacts of, and recovery from, this natural disaster on the stream biogeochemistry and biology were assessed over six months along a longitudinal impact gradient in an urban river. Read More

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November 2013

Voriconazole is cytotoxic at locally delivered concentrations: a pilot study.

Clin Orthop Relat Res 2013 Oct;471(10):3165-70

Banner Good Samaritan Medical Center, Orthopaedic Residency, 901 E Willetta Street, 2nd Floor, Phoenix, AZ, 85006, USA.

Background: Fungal infections are rare but major problems when they involve orthopaedic implants. Preferred treatment in North America is two-staged: resection and then delayed reconstruction, with local delivery of an antifungal between stages. The effect of voriconazole, a hydrophobic antifungal, on local tissues and wound healing is unclear. Read More

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October 2013

Field-scale transport and transformation of carboxymethylcellulose-stabilized nano zero-valent iron.

Environ Sci Technol 2013 Feb 23;47(3):1573-80. Epub 2013 Jan 23.

Institute of Environmental Health, Oregon Health & Science University, 20000 NW Walker Road, Beaverton, Oregon 97006, United States.

The fate of nano zerovalent iron (nZVI) during subsurface injection was examined using carboxymethylcellulose (CMC) stabilized nZVI in a very large three-dimensional physical model aquifer with detailed monitoring using multiple, complementary detection methods. A fluorescein tracer test in the aquifer plus laboratory column data suggested that the very-aggressive flow conditions necessary to achieve 2.5 m of nZVI transport could be obtained using a hydraulically constrained flow path between injection and extraction wells. Read More

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February 2013

Use of relaxation techniques and complementary and alternative medicine by American adults with insomnia symptoms: results from a national survey.

J Clin Sleep Med 2012 Dec 15;8(6):681-91. Epub 2012 Dec 15.

Division of General Medicine and Primary Care, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Brookline, MA 02446, USA.

Introduction: Though relaxation training is recommended for insomnia, national patterns of use remain unknown. Similarly, rates of complementary and alternative medicine (CAM) use by adults with insomnia are not well established. We sought to elucidate the patterns and reasons for use of relaxation techniques and CAM use by adults with insomnia symptoms. Read More

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December 2012

Impact of transiently elevated diastolic pressure on cause of death: 29-year follow-up from the General Practice Hypertension Study Group.

J Hypertens 2013 Jan;31(1):71-6

Imperial College London, London, UK.

Objectives: High blood pressure is known to be associated with higher cardiovascular risk. The emphasis has shifted over time from diastolic pressure to systolic pressure and variability in blood pressure. This study examined the impact of transient and sustained diastolic hypertension on later mortality. Read More

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January 2013

Insulin-like peptide 3 stimulates testosterone secretion in mouse Leydig cells via cAMP pathway.

Regul Pept 2012 Oct 16;178(1-3):102-6. Epub 2012 Jul 16.

Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, Japan.

Testicular Leydig cells secrete insulin-like peptide 3 (INSL3) and express its receptor, RXFP2. However, the effects of INSL3 on endocrine function of Leydig cells are unknown. The present study examines the effects of INSL3 on mouse Leydig cells taking testosterone and cAMP secretions as endpoints. Read More

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October 2012

The interplay of mutations and electronic properties in disease-related genes.

Sci Rep 2012 15;2:272. Epub 2012 Feb 15.

Department of Physics, Tunghai University, 40704 Taichung, Taiwan and The National Center for Theoretical Sciences, 30013 Hsinchu, Taiwan.

Electronic properties of DNA are believed to play a crucial role in many phenomena in living organisms, for example the location of DNA lesions by base excision repair (BER) glycosylases and the regulation of tumor-suppressor genes such as p53 by detection of oxidative damage. However, the reproducible measurement and modelling of charge migration through DNA molecules at the nanometer scale remains a challenging and controversial subject even after more than a decade of intense efforts. Here we show, by analysing 162 disease-related genes from a variety of medical databases with a total of almost 20,000 observed pathogenic mutations, a significant difference in the electronic properties of the population of observed mutations compared to the set of all possible mutations. Read More

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Real-time cell analysis of the cytotoxicity of the components of orthodontic acrylic materials on gingival fibroblasts.

Am J Orthod Dentofacial Orthop 2011 Nov;140(5):e243-9

Assistant professor, Department of Orthodontics, Faculty of Dentistry, İnönü University, Malatya, Turkey.

Introduction: The aim of this study was to evaluate the cytotoxicity of 3 orthodontic acrylic materials and 2 manipulation methods.

Methods: The orthodontic acrylic materials Orthocryl EQ (Dentaurum, Ispringen, Germany), Orthoplast (Vertex Dental, Zeist, The Netherlands), and O-80 (Imicryl, Konya, Turkey) were prepared with 2 polymerization methods (doughing and spray on). Totally, 60 cylinders (5 × 2 mm), fabricated by using a different acrylic and method, were divided into 6 groups. Read More

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November 2011

[Process of exhumation and identification of victims of the 1992-1995 war in the territory of Bosnia and Herzegovina].

Arch Med Sadowej Kryminol 2009 Jul-Sep;59(3):225-31

Z Instytutu ds. Osób Zaginionych, Sarajewo, Bośnia i Hercegowina.

Three and half years of war in Bosnia-Herzegovina in 1992-1995 took lives of thousands of people. About 30,000 of them were accounted for and reported as missing. Fighting and ethnic cleansing took place throughout the country. Read More

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The genome of the Western clawed frog Xenopus tropicalis.

Science 2010 Apr;328(5978):633-6

Department of Energy Joint Genome Institute, Walnut Creek, CA 94598, USA.

The western clawed frog Xenopus tropicalis is an important model for vertebrate development that combines experimental advantages of the African clawed frog Xenopus laevis with more tractable genetics. Here we present a draft genome sequence assembly of X. tropicalis. Read More

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A universal, fully automated high throughput screening assay for pyrophosphate and phosphate release from enzymatic reactions.

Comb Chem High Throughput Screen 2010 Jan;13(1):27-38

Center for Pharmaceutical Biotechnology, University of Illinois at Chicago, IL 60607-7173, USA.

The malachite green assay is often used for measuring the presence of inorganic mono-phosphate concentrations. Some studies have adapted this assay for use in monitoring enzymatic reactions and have suggested its potential use in high throughput screening (HTS). With the increasing availability of laboratory automation, some studies are starting to explore the possibility of conducting limited, semi-automated versions of the assay. Read More

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January 2010

Concurrent bioremediation of perchlorate and 1,1,1-trichloroethane in an emulsified oil barrier.

Authors:
Robert C Borden

J Contam Hydrol 2007 Oct 15;94(1-2):13-33. Epub 2007 Jun 15.

North Carolina State University, Campus Box 7908, Raleigh, NC 27695, USA.

A detailed field pilot test was conducted to evaluate the use of edible oil emulsions for enhanced in situ biodegradation of perchlorate and chlorinated solvents in groundwater. Edible oil substrate (EOS) was injected into a line of ten direct push injection wells over a 2-day period to form a 15-m-long biologically active permeable reactive barrier (bio-barrier). Field monitoring results over a 2. Read More

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October 2007

Evaluation of groundwater flow patterns around a dual-screened groundwater circulation well.

J Contam Hydrol 2007 Aug 6;93(1-4):188-202. Epub 2007 Mar 6.

Oregon Health and Science University, Department of Environmental and Biomolecular Systems, 20000 NW Walker Road, Beaverton, OR 97006, USA.

Dual-screened groundwater circulation wells (GCWs) can be used to remove contaminant mass and to mix reagents in situ. GCWs are so named because they force water in a circular pattern between injection and extraction screens. The radial extent, flux and direction of the effective flow of this circulation cell are difficult to measure or predict. Read More

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Assessing Māori/non-Māori differences in cardiovascular disease risk and risk management in routine primary care practice using web-based clinical decision support: (PREDICT CVD-2).

N Z Med J 2007 Mar 2;120(1250):U2445. Epub 2007 Mar 2.

Section of Epidemiology and Biostatistics, School of Population Health, University of Auckland, Auckland.

Aim: To describe the cardiovascular disease risk factor status and risk management of Māori compared with non-Māori patients opportunistically assessed in routine practice using PREDICT-CVD, an electronic clinical decision support programme.

Methods: In August 2002, a primary healthcare organisation, ProCare, implemented PREDICT-CVD as an opportunistic cardiovascular risk assessment and management programme. Between 2002 and February 2006, over 20,000 cardiovascular risk assessments were undertaken on Māori and non-Māori patients. Read More

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