33,456 results match your criteria 2000 samples

Development and validation of a prediction model for glucocorticoid-associated osteonecrosis of the femoral head by targeted sequencing.

Rheumatology (Oxford) 2021 May 12. Epub 2021 May 12.

Department of Orthopaedics, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.

Objective: To develop and validate a prediction model based on targeted sequencing for glucocorticoid (GC)-associated osteonecrosis of the femoral head (GA-ONFH) in GC-treated adults.

Methods: This two-centre retrospective study was conducted between July 2015 and April 2019 at Zhongshan Hospital (training set) and the Sixth People's Hospital (test set) in Shanghai, China. All patients had a history of GC therapy, with a dose exceeding 2000 mg equivalent prednisone within 6 weeks. Read More

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Reconstruction of ancient microbial genomes from the human gut.

Nature 2021 May 12. Epub 2021 May 12.

Section on Pathophysiology and Molecular Pharmacology, Joslin Diabetes Center, Boston, MA, USA.

Loss of gut microbial diversity in industrial populations is associated with chronic diseases, underscoring the importance of studying our ancestral gut microbiome. However, relatively little is known about the composition of pre-industrial gut microbiomes. Here we performed a large-scale de novo assembly of microbial genomes from palaeofaeces. Read More

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Non-Bullous Pemphigoid: A Single-Center Retrospective Study.

Dermatology 2021 May 12:1-7. Epub 2021 May 12.

Section of Dermatology and Venereology, Department of Medicine, University of Verona, Verona, Italy.

Introduction: Bullous pemphigoid (BP) is an autoimmune disease that typically presents with blisters, but sometimes early lesions may be eczematous, maculopapular, or urticarial. The aim of the present study was to highlight possible differences between typical bullous and non-bullous pemphigoid (NBP) and compare results with the literature. Material & methods: Patients receiving a diagnosis of BP between January 2000 and December 2019 were analyzed. Read More

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Determination of quintrione in rice, rice husk and rice straw and exploration of its dissipation from rice straw.

J Environ Sci Health B 2021 May 12:1-8. Epub 2021 May 12.

College of Plant Protection, Hunan Agricultural University, Southern Regional Collaborative Innovation Center for Grain and Oil Crops, Changsha, China.

A rapid and sensitive method for the identification and quantification of quintrione residue in brown rice, rice husk, and rice straw matrices was developed and validated. Samples were extracted with acetonitrile, purified with octadecylsilane (C18) and graphitized carbon black (GCB) sorbents, and quantified using ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). The developed method exhibited excellent linearity (R ≥ 0. Read More

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Proteomic Imaging of Vestibular Schwannomas and Normal Nerves. Histopathologic Correlations.

Otol Neurotol 2021 May 10. Epub 2021 May 10.

Department of Otolaryngology-Head & Neck Surgery, University Medical Center of Saint-Etienne and Jean Monnet University, Saint-Etienne Clinatec Platform of CEA-LETI Laboratory BrainTech Laboratory Inserm U1205 and University Medical Center of Grenoble Department of Pathology, Cytology, and Tumor Banking (CRB42-TTK), University Medical Center of Saint-Etienne and Jean Monnet University, Saint-Etienne Medicalps Laboratory, Grenoble, France.

Objectives: Proteomic analysis of vestibular schwannoma (VS), non-vestibular schwannoma (NVS), and normal nerve (NN) using mass spectrometry and imaging of matrix assisted laser desorption ionization-time of flight (MALDI-TOF).

Methods: Retrospective, qualitative, and descriptive study on VS, NVS, and NN. Samples were provided by our Tumor Bank. Read More

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Analysis of whole genome sequenced cases and controls shows that the association of variants in , , and with late onset Alzheimer's disease is driven by linkage disequilibrium with ε2/ε3/ε4 alleles.

David Curtis

J Neurogenet 2021 May 10:1-8. Epub 2021 May 10.

UCL Genetics Institute, University College London, London, UK.

Variants in are associated with risk of late onset Alzheimer's disease (LOAD) but the magnitude of the effect has been reported to vary across ancestries. Also, other variants in the region have been reported to show association though it has been unclear whether this was secondary to their linkage disequilibrium with the variants rs429358 and rs7412. Previous analyses of exome-sequenced samples have identified other genes in which rare variants impact risk of disease. Read More

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Neutralizing antibody response against subcutaneously injected bacteriophages in rabbit model.

Virusdisease 2021 Mar 10;32(1):38-45. Epub 2021 Mar 10.

Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005 India.

Bacteriophage therapy is currently experiencing a renaissance. Therapeutic efficacy of bacteriophages depends on phage-bacterial and phage-host interactions. The appearance of neutralizing anti-phage antibody has been speculated to be one of the few reasons for bacteriophage therapy's failure. Read More

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Fast Expansion of the Asian-Pacific Genotype of the Chikungunya Virus in Indonesia.

Front Cell Infect Microbiol 2021 21;11:631508. Epub 2021 Apr 21.

Cirad, UMR 17, Intertryp, Montpellier, France.

Chikungunya is repeatedly affecting Indonesia through successive outbreaks. The Asian genotype has been present in Asia since the late 1950s while the ECSA-IOL (East/Central/South Africa - Indian Ocean Lineage) genotype invaded Asia in 2005. In order to determine the extension of the circulation of the chikungunya virus (CHIKV) in Indonesia, mosquitoes were collected in 28 different sites from 12 Indonesian provinces in 2016-2017. Read More

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Associations of public water system trihalomethane exposure during pregnancy with spontaneous preterm birth and the cervicovaginal microbial-immune state.

Environ Res 2021 May 6:111288. Epub 2021 May 6.

Maternal and Child Health Research Center, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA; Department of Pediatrics, The Children's Hospital of Philadelphia and University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA. Electronic address:

Background: Water total trihalomethanes (TTHMs) are disinfectant byproducts found in municipal water supplies. TTHM exposure has been linked to cancer and may be associated with adverse reproductive outcomes. A non-optimal cervicovaginal microbiota and low cervicovaginal beta-defensin-2 levels are associated with increased risk of spontaneous preterm birth. Read More

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Perceived psychosocial impacts of legalized same-sex marriage: A scoping review of sexual minority adults' experiences.

PLoS One 2021 6;16(5):e0249125. Epub 2021 May 6.

School of Nursing & Department of Psychiatry, Columbia University Irving Medical Center, New York, New York, United States of America.

A growing body of literature provides important insights into the meaning and impact of the right to marry a same-sex partner among sexual minority people. We conducted a scoping review to 1) identify and describe the psychosocial impacts of equal marriage rights among sexual minority adults, and 2) explore sexual minority women (SMW) perceptions of equal marriage rights and whether psychosocial impacts differ by sex. Using Arksey and O'Malley's framework we reviewed peer-reviewed English-language publications from 2000 through 2019. Read More

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Monitoring the carryover of egg proteins in pasta making to support allergen risk management.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2021 May 6:1-9. Epub 2021 May 6.

Department of Food, Environmental and Nutritional Sciences (Defens), University of Milan, Milan, Italy.

Egg proteins are among the major food allergens. Very often, the same pasta-making plants are used for industrial production of egg-based pasta (EBP) and semolina-only pasta (SP), so that residual egg proteins may be present in SP. This calls for defining the amount of semolina pasta that should be discarded when switching production lines. Read More

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Unsupervised manifold alignment for single-cell multi-omics data.

ACM BCB 2020 Sep;2020:1-10

Department of Genome Sciences, University of Washington, Paul G. Allen School of Computer Science and Engineering, University of Washington.

Integrating single-cell measurements that capture different properties of the genome is vital to extending our understanding of genome biology. This task is challenging due to the lack of a shared axis across datasets obtained from different types of single-cell experiments. For most such datasets, we lack corresponding information among the cells (samples) and the measurements (features). Read More

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September 2020

Floating particles with high copper concentration in the sea-surface microlayer.

Environ Sci Pollut Res Int 2021 May 4. Epub 2021 May 4.

School of Spatial Planning and Development, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece.

This study sought to clarify whether suspended particles containing high Cu concentrations are present in the sea-surface microlayer (S-SML). For this reason, suspended particles (10-2000 μm) in the S-SML were collected periodically from a ship mooring pond during 2018-2020, and the acid-soluble Cu concentration in the suspended particles was measured as particulate Cu (P-Cu). The highest concentration of P-Cu in the S-SML of the pond was 75 μg L with a 90th percentile value of 2. Read More

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Chitosan and chitosan/PEG nanoparticles loaded with indole-3-carbinol: Characterization, computational study and potential effect on human bladder cancer cells.

Mater Sci Eng C Mater Biol Appl 2021 May 31;124:112089. Epub 2021 Mar 31.

Tiradentes University - UNIT, Av. Murilo Dantas 300, 49032-490 Aracaju, SE, Brazil; Institute of Technology and Research - ITP, Av. Murilo Dantas 300, 49032-490 Aracaju, SE, Brazil. Electronic address:

Indole-3-carbinol (I3C) is a plant molecule known to be active against several types of cancer, but some chemical characteristics limit its clinical applications. In order to overcome these limitations, polymeric nanoparticles can be used as carrier systems for targeted delivery of I3C. In this study, chitosan and chitosan/polyethylene glycol nanoparticles (CS NP and CS/PEG NP, respectively) were prepared to encapsulate I3C by ionic gelation method. Read More

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Simultaneous Determination of As, Bi, Sb, Se, Te, Hg, Pb and Sn by Small-Sized Electrothermal Vaporization Capacitively Coupled Plasma Microtorch Optical Emission Spectrometry Using Direct Liquid Microsampling.

Molecules 2021 Apr 30;26(9). Epub 2021 Apr 30.

Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University, Arany Janos 11, 400028 Cluj-Napoca, Romania.

The simultaneous determination of chemical vapor-generating elements involving derivatization is difficult even by inductively coupled plasma optical emission spectrometry or mass spectrometry. This study proposes a new direct liquid microsampling method for the simultaneous determination of As, Bi, Se, Te, Hg, Pb, and Sn, using a fully miniaturized set-up based on electrothermal vaporization capacitively coupled plasma microtorch optical emission spectrometry. The method is cost-effective, free from non-spectral interference, and easy to run by avoiding derivatization. Read More

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The genomes of ancient date palms germinated from 2,000 y old seeds.

Proc Natl Acad Sci U S A 2021 May;118(19)

Center for Genomics and Systems Biology, New York University, 129188 Abu Dhabi, United Arab Emirates;

Seven date palm seeds ( L.), radiocarbon dated from the fourth century BCE to the second century CE, were recovered from archaeological sites in the Southern Levant and germinated to yield viable plants. We conducted whole-genome sequencing of these germinated ancient samples and used single-nucleotide polymorphism data to examine the genetics of these previously extinct Judean date palms. Read More

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Cucurbit chlorotic yellows virus infecting Rehmannia glutinosa was detected in China.

Plant Dis 2021 May 2. Epub 2021 May 2.

Yangzhou University, 38043, Department of the Pharmacy, Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu, China;

Rehmannia glutinosa Libosch. is a perennial herbaceous plant of the family Scrophulariaceae. Its roots can be used as traditional Chinese medicine. Read More

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Organohalogens: A persisting burden in Slovenia?

Environ Res 2021 Apr 29;198:111224. Epub 2021 Apr 29.

Jožef Stefan Institute, Jamova Cesta 39, 1000, Ljubljana, Slovenia; Jožef Stefan International Postgraduate School, Jamova Cesta 39, 1000, Ljubljana, Slovenia. Electronic address:

Persistent organic pollutants (POPs) represent a concern for the environment and human health due to their persistence and toxicity. Exposure in Slovenia is geographically differentiated because the country, as part of former Yugoslavia, has a history of industry and regional contamination and is - at the same time - known for its clean nature. The PCB pollution of the Krupa River drew the public's attention to the chemical burden of Slovenians, and the demand for studies has been rising since. Read More

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A systematic review of case reports of hepatic actinomycosis.

Orphanet J Rare Dis 2021 Apr 30;16(1):192. Epub 2021 Apr 30.

Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Background: Hepatic Actinomycosis (HA) is one of the infections that causes disorders in patients when diagnosed untimely and inappropriately.

Methods: Case reports on HA in patients published between 2000 and April 2020 were gathered by carrying out a structured search through PubMed/Medline.

Results: Through a survey of the Medline database, 130 studies were identified and then, 64 cases with HA were included in the final analysis. Read More

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Specific intracellular binding peptide as sPD-L1 antibody mimic: Robust binding capacity and intracellular region specific modulation upon applied to sensing research.

Biosens Bioelectron 2021 Apr 23;185:113269. Epub 2021 Apr 23.

Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai, 200444, PR China. Electronic address:

Programmed death ligand 1 (PD-L1) immune checkpoint has been regarded as a new target for predicting cancer immunotherapy. As a transmembrane protein, PD-L1 has very low blood concentration and is likely to deplete their native activity when separated from the membrane environment due to significant hydrophobic domains, which make it difficult to measure sensitively. The reported PD-L1 aptamers and antibodies are both extracellular region binding molecules with the overlapping binding sites, which seriously limit with the construction of biosensor. Read More

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Quantifying Proteome and Protein Modifications in Activated T Cells by Multiplexed Isobaric Labeling Mass Spectrometry.

Methods Mol Biol 2021 ;2285:297-317

Center for Proteomics and Metabolomics, St. Jude Children's Research Hospital, Memphis, TN, USA.

The dynamic regulation of protein function by altered protein expression and post-translational modifications (PTMs) is essential for T cell function, but it has remained difficult to systemically quantify such events. Mass spectrometry (MS)-based proteomics has become a mainstream tool for comprehensive profiling of proteome and PTMs, especially with the development of multiplexed isobaric labeling methods, such as tandem mass tag (TMT), coupled with high-resolution two-dimensional liquid chromatography and tandem mass spectrometry (LC/LC-MS/MS). Here, we introduce a deep proteomics profiling protocol with an optimized 11-plex TMT-LC/LC-MS/MS platform to quantitate whole proteome, phosphoproteome, acetylome, and methylome in activated T cells. Read More

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January 2021

Use of probiotics to reduce infections and death and prevent colonization with extended-spectrum beta-lactamase (ESBL)-producing bacteria among newborn infants in Tanzania (ProRIDE Trial): study protocol for a randomized controlled clinical trial.

Trials 2021 Apr 29;22(1):312. Epub 2021 Apr 29.

Department of Paediatrics and Adolescence Medicine, University Hospital of North Norway, Tromsø, Norway.

Background: Extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E) has emerged as an urgent global health threat and is by the World Health Organization ranked as priority 1 among pathogens in need of new treatment. Studies have shown high mortality in Tanzanian children with ESBL-E infections. Gut colonization of ESBL-E, which is a potential risk factor of ESBL-E infections, is reported to be very high among children in Tanzania. Read More

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ERIC and WGS Typing of in Slovenia: Investigation of ERIC I Outbreaks.

Insects 2021 Apr 19;12(4). Epub 2021 Apr 19.

Veterinary Faculty, Institute of Microbiology and Parasitology, University of Ljubljana, Gerbičeva 60, SI-1000 Ljubljana, Slovenia.

is the causative agent of American foulbrood (AFB), a fatal disease of honeybee brood. Here, we obtained 506 isolates originating from honey or brood samples and from different geographic regions of Slovenia in the period 2017-2019. In the first part of the study, we conducted ERIC-PCR typing to assess the frequency of ERIC types in Slovenia. Read More

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Triazole-Resistance in Environmental in Latin American and African Countries.

J Fungi (Basel) 2021 Apr 12;7(4). Epub 2021 Apr 12.

Department of Microbiology, Immunology and Transplantation, KU Leuven, 3000 Leuven, Belgium.

Triazole-resistance has been reported increasingly in . An international expert team proposed to avoid triazole monotherapy for the initial treatment of invasive aspergillosis in regions with >10% environmental-resistance, but this prevalence is largely unknown for most American and African countries. Here, we screened 584 environmental samples (soil) from urban and rural locations in Mexico, Paraguay, and Peru in Latin America and Benin and Nigeria in Africa for triazole-resistant . Read More

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Thallium Contamination of Drinking Water: Health Implications in a Residential Cohort Study in Tuscany (Italy).

Int J Environ Res Public Health 2021 Apr 12;18(8). Epub 2021 Apr 12.

Department of Prevention, Health Agency of North-West Tuscany, 56124 Pisa, Italy.

In 2014-2015, concentrations of thallium above the recommended reference value (EPA: 2 µg/L) were measured in some parts of the drinking water distribution system in the municipality of Pietrasanta (Tuscany, Italy). An extensive campaign of water samples and human biomonitoring surveys were implemented to quantify the exposure of population. A residential cohort epidemiological study was carried out on the population of the municipality of Pietrasanta, aimed at comparing the health status of residents in the areas affected by thallium contamination with residents living in the rest of the municipality. Read More

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Inverse 3D Printing with Variations of the Strand Width of the Resulting Scaffolds for Bone Replacement.

Materials (Basel) 2021 Apr 14;14(8). Epub 2021 Apr 14.

Institute for Manufacturing Technologies of Ceramic Components and Composites (IMTCCC), Faculty 07, University of Stuttgart, Allmandring 7b, 70569 Stuttgart, Germany.

The objective of this study was to vary the wall thicknesses and pore sizes of inversely printed 3D molded bodies. Wall thicknesses were varied from 1500 to 2000 to 2500 µm. The pores had sizes of 500, 750 and 1000 µm. Read More

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Lipidomics as a Diagnostic Tool for Prostate Cancer.

Cancers (Basel) 2021 Apr 21;13(9). Epub 2021 Apr 21.

Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 87-100 Toruń, Poland.

The main goal of this study was to explore the phospholipid alterations associated with the development of prostate cancer (PCa) using two imaging methods: matrix-assisted laser desorption ionization with time-of-flight mass spectrometer (MALDI-TOF/MS), and electrospray ionization with triple quadrupole mass spectrometer (ESI-QqQ/MS). For this purpose, samples of PCa tissue ( = 40) were evaluated in comparison to the controls ( = 40). As a result, few classes of compounds, namely phosphatidylcholines (PCs), lysophosphatidylcholines (LPCs), sphingomyelins (SMs), and phosphatidylethanolamines (PEs), were determined. Read More

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Rethinking Manure Application: Increase in Multidrug-Resistant spp. in Agricultural Soil Following Chicken Litter Application.

Microorganisms 2021 Apr 21;9(5). Epub 2021 Apr 21.

Antimicrobial Research Unit, College of Health Science, University of KwaZulu-Natal, Private Bag X54001, Durban 4000, South Africa.

The current study investigated the impact of chicken litter application on the abundance of multidrug-resistant spp. in agricultural soil. Soil samples were collected from five different strategic places on a sugarcane farm before and after manure application for four months. Read More

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Electrochemical Performance of Titania 3D Nanonetwork Electrodes Induced by Pulse Ionization at Varied Pulse Repetitions.

Nanomaterials (Basel) 2021 Apr 21;11(5). Epub 2021 Apr 21.

Silicon Hall, Micro/Nano Manufacturing Facility, Faculty of Engineering and Applied Science, Ontario Tech University, 2000 Simcoe St N, Oshawa, ON L1G 0C5, Canada.

Pulse ionized titania 3D-nanonetworks (T3DN) are emerging materials for fabricating binder-free and carbon-free electrodes for electrochemical energy storage devices. In this article, we investigate the effect of the one of the most important fabrication parameters, pulse frequency, for optimizing supercapacitor efficiency. A series of coin cell batteries with laser-induced electrodes was fabricated; the effect of pulse frequency on oxidation levels and material properties was studied using both experimental and theoretical analysis. Read More

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Description of Plasma Penicillin G Concentrations after Intramuscular Injection in Double-Muscled Cows to Optimize the Timing of Antibiotherapy for Caesarean Section.

Vet Sci 2021 Apr 21;8(5). Epub 2021 Apr 21.

Clinical Department of Ruminants, Faculty of Veterinary Medicine, University of Liège, Quartier Vallée 2, Avenue de Cureghem 7A-7D, 4000 Liège, Belgium.

In order to improve the efficacy of penicillin injection during caesarean section, we aimed to identify the optimal timing of its preoperative administration. A study was conducted in 12 adult, non-pregnant Belgian Blue cows. To evaluate the plasma penicillin concentrations, blood samples were taken from the jugular vein at -5, 15, 30, 45, 60, 120, 240, 480 min relative to the intramuscular (IM) injection of 21,000 IU/kg of body weight of penicillin G. Read More

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