11,617 results match your criteria 18s rrna

Enhancement of euryhaline biomass production for improving biogas generation through anaerobic co-digestion with carbon rich substrate.

3 Biotech 2021 May 3;11(5):251. Epub 2021 May 3.

Department of Microbiology, Punjab Agricultural University, Ludhiana, Punjab 141004 India.

The microalga was isolated from Muktsar, the southwestern zone of Indian Punjab and identified as BGLR5 (MF661929) by 18S rRNA sequence analysis. The optimization of various cultural factors by the Plackett-Burman and central composite (CCD) designs helped in discerning the significant cultural factors for the increased production of biomass and other functional components (chlorophyll, carbohydrate, lipid and protein). The optimal cultural conditions as per the model were pH 9. Read More

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Mycoplankton Biome Structure and Assemblage Processes Differ Along a Transect From the Elbe River Down to the River Plume and the Adjacent Marine Waters.

Front Microbiol 2021 23;12:640469. Epub 2021 Apr 23.

Molecular Ecology Group, University of Bremen, FB2, Bremen, Germany.

Rivers are transport systems and supply adjacent ecosystems with nutrients. They also serve human well-being, for example as a source of food. Microorganism biodiversity is an important parameter for the ecological balance of river ecosystems. Read More

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Application of an indigenous microorganisms-based fixed-bed GAC-biofilm reactor for passive and sustainable treatment of oil sands process water through combined adsorption and biodegradation processes.

Chemosphere 2021 Apr 30;280:130635. Epub 2021 Apr 30.

Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta, T6G 1H9, Canada. Electronic address:

In this study, a fixed-bed biofilm reactor (biofilter) was developed and applied for oil sands process water (OSPW) remediation by using granular activated carbon (GAC) as packing media. Using quantitative polymerase chain reaction (qPCR) detection, the total bacterial copy number (16S) in the GAC biofiltration system was found to reach a relatively stable level (1.3 ± 0. Read More

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Plantar mechanical stimulation attenuates protein synthesis decline in disused skeletal muscle via modulation of nitric oxide level.

Sci Rep 2021 May 7;11(1):9806. Epub 2021 May 7.

Myology Laboratory, Institute of Biomedical Problems RAS, Khoroshevskoe shosse 76a, Moscow, Russian Federation, 123007.

Both research conducted under microgravity conditions and ground-based space analog studies have shown that air pump-based plantar mechanical stimulation (PMS) of cutaneous mechanoreceptors of the sole of the foot is able to increase neuromuscular activity in the musculature of the lower limbs. This type of stimulation is able to attenuate unloading-induced skeletal muscle atrophy and impaired muscle function. The aim of the present study was to evaluate the effects of PMS on anabolic signaling pathways in rat soleus muscle following 7-day hindlimb suspension (HS) and to elucidate if the effects of PMS on anabolic processes would be NO-dependent. Read More

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Evolution of host use in fungivorous ciid beetles (Coleoptera: Ciidae): molecular phylogeny focusing on Japanese taxa.

Mol Phylogenet Evol 2021 May 4:107197. Epub 2021 May 4.

Department of Zoology, Graduate School of Science, Kyoto University, Kitashirakawa-oiwake, Sakyo, Kyoto 606 8502 Japan.

Consumer-resource interactions between trophic levels are ubiquitous and important factors in shaping the diversity of insects. However, dietary patterns such as host specificity and conservatism have been insufficiently examined in fungivorous insects. Here we reconstructed the evolutionary history of host use in fungivorous ciid beetles (Coleoptera: Ciidae) and tested for host conservatism. Read More

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A novel duplex qPCR assay for stepwise detection of multiple Perkinsea protistan infections of amphibian tissues.

R Soc Open Sci 2021 Mar 17;8(3):202150. Epub 2021 Mar 17.

Department of Zoology, University of Oxford, 11a Mansfield Road, Oxford OX1 3SZ, UK.

Alveolate protists within the phylum Perkinsea have been found to infect amphibians across a broad taxonomic and geographic range. Phylogenetic analysis has suggested the existence of two clades of amphibian Perkinsea: a putatively non-pathogenic clade linked to asymptomatic infections of tadpoles in Africa, Europe and South America, and a putatively pathogenic clade linked to disease and mass mortality events of tadpoles in North America. Here, we describe the development of a duplex TaqMan qPCR assay to detect and discriminate between rDNA sequences from both clades of Perkinsea in amphibian tissues. Read More

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Application of High-Resolution Melting (HRM) Technique towards the Detection of Asymptomatic Malaria in a Malaria Endemic Area of Southeastern Iran under Elimination Program.

J Arthropod Borne Dis 2020 Dec 31;14(4):353-362. Epub 2020 Dec 31.

Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran.

Background: Asymptomatic malaria, which usually exists in low parasitemia, acts as the Plasmodium species reservoirs contributing towards malaria transmission. This situation hinders malaria elimination programs in endemic areas, thus necessitating an active case detection with a high sensitive method and treatment of cases. This is why we used a High Resolution Melting (HRM) assay to monitor the trend of asymptomatic malaria in a malaria endemic area of Iran which is under elimination program. Read More

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December 2020

Macronuclear Plasticity in Two South American Populations of Spirostomum (Ciliophora, Heterotrichea) Warns About its Use for Species Classification: Revision and New Insights.

Protist 2021 Mar 11;172(2):125803. Epub 2021 Mar 11.

Laboratório de Protistologia, Instituto de Biologia, CCS, Universidade Federal do Rio de Janeiro (UFRJ), Ilha do Fundão, Rio de Janeiro, Brazil. Electronic address:

Spirostomum is a widely distributed heterotrichean genus composed of well-known species with described ecology and phylogenetic affinities. The morphological classification of Spirostomum species is mostly based on the body size/shape, number of cortical granule rows and macronuclear characteristics. These features along with molecular phylogenies based on ribosomal genes divide the genus into two phylogroups, one including species with a compact macronucleus, and another including species with a moniliform macronucleus. Read More

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Comprehensive single-PCR 16S and 18S rRNA community analysis validated with mock communities, and estimation of sequencing bias against 18S.

Environ Microbiol 2021 May 2. Epub 2021 May 2.

Department of Biological Sciences, University of Southern California, Los Angeles, California, 90089-0371, USA.

Universal primers for SSU rRNA genes allow profiling of natural communities by simultaneously amplifying templates from Bacteria, Archaea, and Eukaryota in a single PCR reaction. Despite the potential to show relative abundance for all rRNA genes, universal primers are rarely used, due to various concerns including amplicon length variation and its effect on bioinformatic pipelines. We thus developed 16S and 18S rRNA mock communities and a bioinformatic pipeline to validate this approach. Read More

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Phylogenetic study of and in sheep from Egypt: Molecular evidence and genetic characterization.

Vet World 2021 Mar 13;14(3):634-639. Epub 2021 Mar 13.

Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, 51452 Qassim, Saudi Arabia.

Background And Aim: Ovine theileriosis caused by and is an important infectious disease affecting small ruminants in regions of the tropic and subtropic zones. There is limited studies about ovine theileriosis in Egypt; so the present study aims to assess the occurrence of ovine theileriosis in Egypt at the molecular level.

Materials And Methods: Blood samples were collected from 115 randomly selected sheep, which were apparently healthy; the ages of the sampled sheep ranged from 1 to 5 years old, from a local breed (barkae and balade), and showed no symptoms indicating infection with spp. Read More

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Methods for Identifying Microbial Natural Product Compounds that Target Kinetoplastid RNA Structural Motifs by Homology and De Novo Modeled 18S rRNA.

Int J Mol Sci 2021 Apr 26;22(9). Epub 2021 Apr 26.

Department of Biochemistry, University of Nairobi, Nairobi 00200-30197, Kenya.

The development of novel anti-infectives against Kinetoplastids pathogens targeting proteins is a big problem occasioned by the antigenic variation in these parasites. This is also a global concern due to the zoonosis of these parasites, as they infect both humans and animals. Therefore, we need not only to create novel antibiotics, but also to speed up the development pipeline for these antibiotics. Read More

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A Versatile qPCR for Diagnosis of Leporid Gammaherpesvirus 5 Using Evagreen or Taqman Technologies.

Viruses 2021 04 20;13(4). Epub 2021 Apr 20.

Instituto Nacional de Investigação Agrária e Veterinária, Av. da República, Quinta do Marquês, 2780-157 Oeiras, Portugal.

In late 2019, the first herpesvirus in the genus Lepus, named leporid gammaherpesvirus 5 (LeHV-5) was described. At the time, herpetic typical lesions were observed in hares infected by the myxoma virus, which is known to induce immunosuppression. Though the real impact of LeHV-5 is still poorly understood, since it affects reproduction, it poses an additional threat to the already fragile populations of Iberian hare, demanding prevalence investigations. Read More

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Diverse Genotypes and Species of in Wild Rodent Species from the West Coast of the USA and Implications for Raw Produce Safety and Microbial Water Quality.

Microorganisms 2021 Apr 17;9(4). Epub 2021 Apr 17.

Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.

spp. are protozoan parasites that infect perhaps all vertebrate animals, with a subset of species and genotypes that function as food- and waterborne pathogens. The objective of this work was to collate the species and genotypes from common wild rodents on the west coast of the USA and update the information regarding the zoonotic potential of from these ubiquitous wild species. Read More

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Molecular Identification of Isolated from Arabian Camels () in Riyadh and Al-Qassim, Saudi Arabia.

Animals (Basel) 2021 Apr 17;11(4). Epub 2021 Apr 17.

Department of Biological Science, Faculty of Science and Humanities, Shaqra University, Ad-Dawadimi 11911, Saudi Arabia.

We analyzed the blood from 400 one-humped camels, () in Riyadh and Al-Qassim, Saudi Arabia to determine if they were infected with the parasite spp. Polymerase chain reaction (PCR) targeting the internal transcribed spacer 1 (ITS1) gene was used to detect the prevalence of spp. in the camels. Read More

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An Introduction to Next Generation Sequencing Bioinformatic Analysis in Gut Microbiome Studies.

Biomolecules 2021 Apr 2;11(4). Epub 2021 Apr 2.

Department of Medicine, University of California San Diego, La Jolla, CA 92093, USA.

The gut microbiome is a microbial ecosystem which expresses 100 times more genes than the human host and plays an essential role in human health and disease pathogenesis. Since most intestinal microbial species are difficult to culture, next generation sequencing technologies have been widely applied to study the gut microbiome, including 16S rRNA, 18S rRNA, internal transcribed spacer (ITS) sequencing, shotgun metagenomic sequencing, metatranscriptomic sequencing and viromic sequencing. Various software tools were developed to analyze different sequencing data. Read More

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Detection of Free-Living Amoebae and Their Intracellular Bacteria in Borehole Water before and after a Ceramic Pot Filter Point-of-Use Intervention in Rural Communities in South Africa.

Int J Environ Res Public Health 2021 Apr 8;18(8). Epub 2021 Apr 8.

Environmental Health, Domestic Hygiene and Microbial Pathogens Research Group, Department of Microbiology, University of Venda, Thohoyandou 1950, South Africa.

Free-living amoebae (FLA) are ubiquitous in nature, whereas amoeba-resistant bacteria (ARB) have evolved virulent mechanisms that allow them to resist FLA digestion mechanisms and survive inside the amoeba during hostile environmental conditions. This study assessed the prevalence of FLA and ARB species in borehole water before and after a ceramic point-of-use intervention in rural households. A total of 529 water samples were collected over a five-month period from 82 households. Read More

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The final step of 40S ribosomal subunit maturation is controlled by a dual key lock.

Elife 2021 Apr 28;10. Epub 2021 Apr 28.

Molecular, Cellular and Developmental biology department (MCD), Centre de Biologie Integrative, University of Toulouse, Toulouse, France.

Preventing premature interaction of pre-ribosomes with the translation apparatus is essential for translational accuracy. Hence, the final maturation step releasing functional 40S ribosomal subunits, namely processing of the 18S ribosomal RNA 3' end, is safeguarded by the protein DIM2, which both interacts with the endoribonuclease NOB1 and masks the rRNA cleavage site. To elucidate the control mechanism that unlocks NOB1 activity, we performed cryo-EM analysis of late human pre-40S particles purified using a catalytically-inactive form of the ATPase RIO1. Read More

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Urinary Cell mRNA Profiles Predictive of Human Kidney Allograft Status.

Clin J Am Soc Nephrol 2021 Apr 27. Epub 2021 Apr 27.

Division of Nephrology and Hypertension, Weill Cornell Department of Medicine, New York, New York

Immune monitoring of kidney allograft recipients and personalized therapeutics may help reach the aspirational goal of "one transplant for life." The invasive kidney biopsy procedure, the diagnostic tool of choice, has become safer and the biopsy classification more refined. Nevertheless, biopsy-associated complications, interobserver variability in biopsy specimen scoring, and costs continue to be significant concerns. Read More

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Obtaining deeper insights into microbiome diversity using a simple method to block host and non-targets in amplicon sequencing.

Mol Ecol Resour 2021 Apr 27. Epub 2021 Apr 27.

Plant Microbiosis Lab, Department of Microbiology, Friedrich-Schiller University, Jena, Germany.

Profiling diverse microbiomes is revolutionizing our understanding of biological mechanisms and ecologically relevant problems, including metaorganism (host+microbiome) assembly, functions and adaptation. Amplicon sequencing of multiple conserved, phylogenetically informative loci has therefore become an instrumental tool for many researchers. Investigations in many systems are hindered, however, since essential sequencing depth can be lost by amplification of non-target DNA from hosts or overabundant microorganisms. Read More

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An integrative description of Diploechiniscus oihonnae/ (Richters, 1903) population from near the original type locality in Merok (Norway).

Zootaxa 2021 Apr 21;4964(1):zootaxa.4964.1.4. Epub 2021 Apr 21.

Department of Animal Taxonomy and Ecology, Faculty of Biology, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland..

In the present study, we used integrative taxonomy to describe a population of Diploechiniscus oihonnae (Richters, 1903) from the neotype locality in Merok (Norway). We found no differences in the chaetotaxy formula between the life stages and sex of Dpl. oihonnae. Read More

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A new amphibious species of the genus emScolopendra/em Linnaeus, 1758 (Scolopendromorpha, Scolopendridae) from the Ryukyu Archipelago and Taiwan.

Zootaxa 2021 Apr 12;4952(3):zootaxa.4952.3.3. Epub 2021 Apr 12.

Systematic Zoology Laboratory, Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Minami-osawa 1-1 Hachioji-shi, Tokyo, 192-0397 Japan.

In Japan and Taiwan, five valid species of the genus Scolopendra Linnaeus, 1758 have been described: S. morsitans Linnaeus, 1758, S. subspinipes Leach, 1816, S. Read More

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Molecular characterization of free-living nematodes from Kermadec Trench (Nematoda: Aegialoalaimidae, Xyalidae) with description of emAegialoalaimus/em emtereticauda/em n. sp.

Zootaxa 2021 Mar 25;4949(2):zootaxa.4949.2.7. Epub 2021 Mar 25.

National Institute of Water and Atmospheric Research (NIWA) Ltd, Wellington, New Zealand.

The Kermadec Trench is the world's fifth deepest trench and extends from approximately 26 to 36°S near the northeastern tip of New Zealand's North Island. Here, we describe a new species of Aegialoalaimus, a nematode genus with unusual buccal cavity and pharynx morphology, from a site at 9540 metres water depth in Kermadec Trench, and provide the first SSU and D2-D3 of LSU sequences for Aegialoalaimus, Manganonema, Metasphaerolaimus constrictus and Daptonema amphorum. Aegialoalaimus tereticauda n. Read More

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Salinity is the major driver of the global eukaryotic community structure in fish-canning wastewater treatment plants.

J Environ Manage 2021 Apr 23;290:112623. Epub 2021 Apr 23.

Universidad de Granada. Facultad de Farmacia. Dpto. de Microbiología, Spain; Universidad de Granada. Instituto del Agua. Sección Microbiología y Tecnologías Ambientales, Spain.

Fish-canning wastewater is characterized frequently by a high content of salt (NaCl), making its treatment particularly difficult; however, the knowledge of the effect of NaCl on eukaryotic communities is very limited. In the present study, the global diversity of eukaryotes in activated sludges (AS) from 4 different wastewater treatment plants (WWTPs) treating fish-canning effluents varying in salinity (0.47, 1. Read More

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Distribution and diversity of eukaryotic microalgae in Kuwait waters assessed using 18S rRNA gene sequencing.

PLoS One 2021 26;16(4):e0250645. Epub 2021 Apr 26.

Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, Kuwait, Kuwait.

The microbial communities play a crucial role in ecosystem functioning through interactions among individuals and taxonomic groups in a highly dynamic marine ecosystem. The structure and functioning of the microbial communities are often influenced by the changes in the surrounding environment. Monitoring the microbial diversity of the marine ecosystem helps to understand spatial patterns of microbial community and changes due to season, climate, and various drivers of biological diversity. Read More

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[The feeding of sp. on two microalgae species.]

Ying Yong Sheng Tai Xue Bao 2021 Apr;32(4):1479-1488

Ornamental Aquarium Engineering Research Centre in Universities of Fujian Province/Fisheries College of Jimei University, Xiamen 361021, Fujian, China.

In this study, we investigated whether sp. feed on two common microalgae, and , using the methods of carbon clearance rate, DNA marker, and histological analyses. The results showed that carbon clearance rate of by sp. Read More

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species infection in patients seeking treatment for diarrhea and abdominal discomfort in Mukuru informal settlement in Nairobi, Kenya.

Food Waterborne Parasitol 2021 Jun 29;23:e00122. Epub 2021 Mar 29.

Centre for Microbiology Research, Kenya Medical Research Institute, P.O. Box 19464 00202, Nairobi, Kenya.

is the only pathogenic species of the genus and is morphologically identical to / ( complex) hence cannot be microscopically differentiated. The other spp. found in humans ( and ) can be differentiated morphologically from this complex. Read More

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Two new species of (Nematoda: Onchocercidae) parasitising anuran amphibians in South Africa.

Int J Parasitol Parasites Wildl 2021 Apr 18;14:298-307. Epub 2021 Mar 18.

I.I. Schmalhausen Institute of Zoology NAS of Ukraine, 15 B. Khmelnytskogo str., 01030, Kyiv, Ukraine.

The genus was recently erected for a single species, from bufonid hosts in South Africa. In present study, we discovered two undescribed species of , namely n. sp. Read More

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Integrating metagenetics and high-throughput screening for bioprospecting marine thraustochytrids producers of long-chain polyunsaturated fatty acids.

Bioresour Technol 2021 Apr 15;333:125176. Epub 2021 Apr 15.

Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), 81531-970 Curitiba, PR, Brazil. Electronic address:

Omega-3 produced by marine thraustochytrids has appeared as an alternative to fish oil and an eco-friendly solution to overfishing. Herein, an integrative analysis of metagenetics and high-throughput screening was used for bioprospecting marine thraustochytrids from southern Brazil mangrove and coastal seawater. All sampled environments showed biodiversity and abundance of SAR clade. Read More

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First report of molecular and phylogenetic analysis of Physaloptera praeputialis in naturally infected stray cats from India.

Parasitol Res 2021 Apr 24. Epub 2021 Apr 24.

Referral Veterinary Diagnostic and Extension Centre, Lala Lajpat Rai University of Veterinary and Animal Sciences, Karnal, Haryana, 132001, India.

Nematodes of the genus Physaloptera are globally distributed and infect a multitude of hosts. Their life cycle involves orthopterans and coleopterans as intermediate hosts. The morphological characters alone are inadequate to detect and differentiate Physaloptera spp. Read More

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First next-generation sequencing data for Haploporidae (Digenea: Haploporata): characterization of complete mitochondrial genome and ribosomal operon for Parasaccocoelium mugili Zhukov, 1971.

Parasitol Res 2021 Apr 24. Epub 2021 Apr 24.

Laboratory of Ecology and Evolutionary Biology of Aquatic Organisms, Far Eastern Federal University, Vladivostok, Russia.

The first data on a whole mitochondrial genome of Haploporidae, Parasaccocoelium mugili (Digenea: Haploporata: Haploporidae) was generated using the next-generation sequencing (NGS) approach. We sequenced the complete mitochondrial DNA (mtDNA) and ribosomal operon of Parasaccocoelium mugili, intestine parasite of mullet fish. The mtDNA of P. Read More

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