238 results match your criteria 1200 differentially

Mapping the molecular basis for growth related phenotypes in industrial producer CHO cell lines using differential proteomic analysis.

BMC Biotechnol 2021 Jul 23;21(1):43. Epub 2021 Jul 23.

National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.

Background: The ability to achieve high peak viable cell density earlier in CHO cell culture and maintain an extended cell viability throughout the production process is highly desirable to increase recombinant protein yields, reduce host cell impurities for downstream processing and reduce the cost of goods. In this study we implemented label-free LC-MS/MS proteomic profiling of IgG4 producing CHO cell lines throughout the duration of the cell culture to identify differentially expressed (DE) proteins and intracellular pathways associated with the high peak viable cell density (VCD) and extended culture VCD phenotypes.

Results: We identified key pathways in DNA replication, mitotic cell cycle and evasion of p53 mediated apoptosis in high peak VCD clonally derived cell lines (CDCLs). Read More

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Differential expression of miRNAs and functional role of mir-200a in high and low productivity CHO cells expressing an Fc fusion protein.

Biotechnol Lett 2021 Aug 16;43(8):1551-1563. Epub 2021 Jun 16.

National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.

Objectives: We used miRNA and proteomic profiling to understand intracellular pathways that contribute to high and low specific productivity (Qp) phenotypes in CHO clonally derived cell lines (CDCLs) from the same cell line generation project.

Results: Differentially expressed (DE) miRNAs were identified which are predicted to target several proteins associated with protein folding. MiR-200a was found to have a number of predicted targets associated with the unfolded protein response (UPR) which were shown to have decreased expression in high Qp CDCLs and have no detected change at the mRNA level. Read More

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Arylated gold nanoparticles have no effect on the adipogenic differentiation of MG-63 cells nor regulate any key signaling pathway during the differentiation.

BMC Res Notes 2021 May 19;14(1):192. Epub 2021 May 19.

Department of Chemistry, College of Sciences, University of Sharjah, Sharjah, 27272, UAE.

Objective: MG-63 cells that have osteoblastic and adipogenic differentiation potential were evaluated for internalization, and adipogenic differentiation in the presence and absence of the covalently functionalized aryl gold nanoparticles (AuNPs-CH-4-COOH).

Results: Inductively coupled plasma, flow cytometry and confocal microscopy analyses confirmed that gold nanoparticles were easily internalized by MG-63 cells. The MG-63 cells were differentiated into adipocytes without gold-aryl nanoparticles and with the gold-aryl nanoparticles at 5 µM concentration in both induction and maintenance media. Read More

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Proteomics and Machine Learning Approaches Reveal a Set of Prognostic Markers for COVID-19 Severity With Drug Repurposing Potential.

Front Physiol 2021 27;12:652799. Epub 2021 Apr 27.

Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India.

The pestilential pathogen SARS-CoV-2 has led to a seemingly ceaseless pandemic of COVID-19. The healthcare sector is under a tremendous burden, thus necessitating the prognosis of COVID-19 severity. This in-depth study of plasma proteome alteration provides insights into the host physiological response towards the infection and also reveals the potential prognostic markers of the disease. Read More

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Exposure to sevoflurane results in changes of transcription factor occupancy in sperm and inheritance of autism.

Biol Reprod 2021 May 12. Epub 2021 May 12.

Department of Human Genetics, Emory University School of Medicine, 615 Michael St, Atlanta, GA 30322, USA.

One in 54 children in the U.S. is diagnosed with Autism Spectrum Disorder (ASD). Read More

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MYOD1 inhibits avian adipocyte differentiation via miRNA-206/KLF4 axis.

J Anim Sci Biotechnol 2021 May 5;12(1):55. Epub 2021 May 5.

National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, MARA, College of Animal Science and Technology, China Agricultural University, Yuanmingyuan West Road No. 2, Beijing, 100193, China.

Background: A considerable number of muscle development-related genes were differentially expressed in the early stage of avian adipocyte differentiation. However, the functions of them in adipocyte differentiation remain largely known. In this study, the myoblast determination protein 1 (MYOD1) was selected as a representative of muscle development. Read More

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A LysR-type transcriptional regulator controls the expression of numerous small RNAs in Agrobacterium tumefaciens.

Mol Microbiol 2021 Jul 2;116(1):126-139. Epub 2021 Mar 2.

Microbial Biology, Ruhr University Bochum, Bochum, Germany.

Small RNAs (sRNAs) are universal posttranscriptional regulators of gene expression and hundreds of sRNAs are frequently found in each and every bacterium. In order to coordinate cellular processes in response to ambient conditions, many sRNAs are differentially expressed. Here, we asked how these small regulators are regulated using Agrobacterium tumefaciens as a model system. Read More

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American lobster postlarvae alter gene regulation in response to ocean warming and acidification.

Ecol Evol 2021 Jan 29;11(2):806-819. Epub 2020 Dec 29.

Darling Marine Center University of Maine School of Marine Sciences Walpole ME USA.

Anthropogenic carbon emissions released into the atmosphere is driving rapid, concurrent increases in temperature and acidity across the world's oceans. Disentangling the interactive effects of warming and acidification on vulnerable life stages is important to our understanding of responses of marine species to climate change. This study evaluates the interactive effects of these stressors on the acute response of gene expression of postlarval American lobster (), a species whose geographic range is warming and acidifying faster than most of the world's oceans. Read More

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January 2021

Human Transcriptomic Response to the VSV-Vectored Ebola Vaccine.

Vaccines (Basel) 2021 Jan 20;9(2). Epub 2021 Jan 20.

Laboratory of Molecular Microbiology and Biotechnology (LAMMB), Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy.

Ebolavirus Disease (EVD) is a severe haemorrhagic fever that occurs in epidemic outbreaks, with a high fatality rate and no specific therapies available. rVSVΔG-ZEBOV-GP (Ervebo), a live-attenuated recombinant vesicular stomatitis virus vector expressing the glycoprotein G of Zaire Ebolavirus, is the first vaccine approved for prevention of EVD. Both innate and adaptive responses are deemed to be involved in vaccine-induced protection, yet the mechanisms are not fully elucidated. Read More

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January 2021

Actin at stereocilia tips is regulated by mechanotransduction and ADF/cofilin.

Curr Biol 2021 Mar 4;31(6):1141-1153.e7. Epub 2021 Jan 4.

Department of Biology, Indiana University-Purdue University Indianapolis, 723 West Michigan Street, Indianapolis, IN 46202, USA. Electronic address:

Stereocilia on auditory sensory cells are actin-based protrusions that mechanotransduce sound into an electrical signal. These stereocilia are arranged into a bundle with three rows of increasing length to form a staircase-like morphology that is required for hearing. Stereocilia in the shorter rows, but not the tallest row, are mechanotransducing because they have force-sensitive channels localized at their tips. Read More

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Genomic atlases of introgression and differentiation reveal breeding footprints in Chinese cultivated rice.

J Genet Genomics 2020 10 25;47(10):637-649. Epub 2020 Nov 25.

State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

The long history of cultivation and breeding has left a variety of footprints in the genomes of Asian cultivated rice (Oryza sativa L.). In this study, we focus on two types of genomic footprints, introgression and differentiation, in a population of more than 1200 Chinese rice accessions. Read More

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October 2020

High-resolution analyses of human sperm dynamic methylome reveal thousands of novel age-related epigenetic alterations.

Clin Epigenetics 2020 12 14;12(1):192. Epub 2020 Dec 14.

Department of Pharmacology and Therapeutics, McGill University, 3655 Promenade Sir William Osler, Montreal, QC, H3G 1Y6, Canada.

Background: Children of aged fathers are at a higher risk of developing mental disorders. Alterations in sperm DNA methylation have been implicated as a potential cause. However, age-dependent modifications of the germ cells' epigenome remain poorly understood. Read More

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December 2020

Prolonged, Controlled Daytime versus Delayed Eating Impacts Weight and Metabolism.

Curr Biol 2021 Feb 30;31(3):650-657.e3. Epub 2020 Nov 30.

Division of Sleep and Chronobiology, Department of Psychiatry, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, USA; Biological Rhythms Research Laboratory, Department of Psychiatry and Behavioral Sciences, Rush University Medical Center, Chicago, IL 60612, USA.

A delayed eating schedule is associated with increased risk of obesity and metabolic dysfunction in humans. However, there are no prolonged, highly controlled experimental studies testing the effects of meal timing on weight and metabolism in adults with a body mass index (BMI) of 19-27 kg/m. Twelve healthy adults (age: 26. Read More

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February 2021

Yellow-fruited phenotype is caused by 573 bp insertion at 5' UTR of YFT1 allele in yft1 mutant tomato.

Plant Sci 2020 Nov 16;300:110637. Epub 2020 Aug 16.

Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China; Joint Tomato Research Institute, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China. Electronic address:

The yft1 tomato mutant has a yellow-fruited phenotype controlled by a recessive gene of YFT1 allele, which has been shown by map-based cloning to be a homolog of ETHYLENE INSENSITIVE 2 (EIN2). Genetic lesion of YFT1 allele in yft1 is attributed to a 573 bp DNA fragment (IF) insertion at 1,200 bp downstream of the transcription start site. Transcriptomic analysis revealed that YFT1 lesion resulted in 5,053 differentially expressed genes (DEGs) in yft1 pericarp compared with the M82 wild type cultivar. Read More

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November 2020

Orchestrates Gene Regulatory Variation in Mouse Ventral Midbrain During Aging.

Front Genet 2020 23;11:566734. Epub 2020 Sep 23.

Department of Life Sciences and Medicine, University of Luxembourg, Belvaux, Luxembourg.

Dopaminergic neurons in the midbrain are of particular interest due to their role in diseases such as Parkinson's disease and schizophrenia. Genetic variation between individuals can affect the integrity and function of dopaminergic neurons but the DNA variants and molecular cascades modulating dopaminergic neurons and other cells types of ventral midbrain remain poorly defined. Three genetically diverse inbred mouse strains - C57BL/6J, A/J, and DBA/2J - differ significantly in their genomes (∼7 million variants), motor and cognitive behavior, and susceptibility to neurotoxins. Read More

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September 2020

Impact of Ocean Warming and Acidification on Symbiosis Establishment and Gene Expression Profiles in Recruits of Reef Coral .

Front Microbiol 2020 30;11:532447. Epub 2020 Sep 30.

CAS Key Laboratory of Tropical Marine Bio-resources and Ecology; Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.

The onset of symbiosis and the early development of most broadcast spawning corals play pivotal roles in recruitment success, yet these critical early stages are threatened by multiple stressors. However, molecular mechanisms governing these critical processes under ocean warming and acidification are still poorly understood. The present study investigated the interactive impact of elevated temperature (∼28. Read More

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September 2020

Physiological Characteristics of Photosynthesis in Yellow-Green, Green and Dark-Green Chinese Kale ( L. var. Musil.) under Varying Light Intensities.

Plants (Basel) 2020 Jul 30;9(8). Epub 2020 Jul 30.

Department of Life Sciences, National Chung-Hsing University, Taichung40227, Taiwan.

The objective of this work was to study physiological characteristics and photosynthetic apparatus in differentially pigmented leaves of three Chinese kale cultivars. Chlorophyll (Chl) fluorescence and photochemical reflectance index (PRI) measurements in green, yellow-green, and dark-green cultivars in response to varying light intensities. As light intensity increased from 200 to 2000 photosynthetic photon flux density (PPFD), fraction of light absorbed in photosystem (PS) II and PRI values in all plants were strongly lowered, but fraction of light absorbed in PSII dissipated via thermal energy dissipation and non-photochemical quenching (NPQ) values in all plants wereremarkably elevated. Read More

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Molecular and metabolomic changes in the proximal colon of pigs infected with Trichuris suis.

Sci Rep 2020 07 30;10(1):12853. Epub 2020 Jul 30.

United States Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Diet, Genomics, and Immunology Laboratory, Beltsville, USA.

The pig whipworm Trichuris suis is important in swine production because of its negative effects on pig performance and, notably, to some humans with inflammatory bowel disease as a therapeutic agent that modulates inflammation. The proximal colon of T. suis-infected pigs exhibited general inflammation around day 21 after inoculation with infective eggs that is transcriptionally characterized by markers of type-2 immune activation, inflammation, cellular infiltration, tissue repair enzymes, pathways of oxidative stress, and altered intestinal barrier function. Read More

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Transcriptome analysis provides insights into the effects of myo-inositol on the turbot Scophthalmus maximus.

Fish Shellfish Immunol 2020 Nov 23;106:691-704. Epub 2020 Jul 23.

Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding, Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China. Electronic address:

Myo-inositol is an essential vitamin for most animals, and it can modulate multiple physiological functions. In this study, we performed transcriptome gene expression profiling of gill tissue from turbot Scophthalmus maximus fed different concentrations of myo-inositol (0, 300, 600, 900, 1200 mg/kg). Results of expression tendency analysis, Weighted Gene Co-Expression Network Analysis (WGCNA), integrated transcriptome analyses, and KEGG annotation analysis of all differentially expressed genes (DEGs) demonstrated that the cytokine-cytokine receptor interaction played a core role in effects of myo-inositol on turbot, which was followed by the Jak-STAT signaling pathway. Read More

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November 2020

YYFZBJS ameliorates colorectal cancer progression in Apc mice by remodeling gut microbiota and inhibiting regulatory T-cell generation.

Cell Commun Signal 2020 07 16;18(1):113. Epub 2020 Jul 16.

Department of Medical Oncology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, 528 Zhangheng Rd, Shanghai, 201203, P.R. China.

Background: Progression of Colorectal cancer (CRC) is influenced by single or compounded environmental factors. Accumulating evidence shows that microbiota can influence the outcome of cancer immunotherapy. T cell, one of the main populations of effector immune cells in antitumor immunity, has been considered as a double-edged sword during the progression of CRC. Read More

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Inflammation Differentially Modulates the Biological Features of Adult Derived Human Liver Stem/Progenitor Cells.

Cells 2020 07 8;9(7). Epub 2020 Jul 8.

Laboratory of Pediatric Hepatology and Cell Therapy, Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1200 Brussels, Belgium.

The progression of mesenchymal stem cell-based therapy from concept to cure closely depends on the optimization of conditions that allow a better survival and favor the cells to achieve efficient liver regeneration. We have previously demonstrated that adult-derived human liver stem/progenitor cells (ADHLSC) display significant features that support their clinical development. The current work aims at studying the impact of a sustained pro-inflammatory environment on the principal biological features of ADHLSC in vitro. Read More

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Modulation of VEGF Expression and Oxidative Stress Response by Iodine Deficiency in Irradiated Cancerous and Non-Cancerous Breast Cells.

Int J Mol Sci 2020 May 31;21(11). Epub 2020 May 31.

Radiobiology Unit, Belgian Nuclear Research Centre (SCK•CEN), 2400 Mol, Belgium.

Breast cancer remains a major concern and its physiopathology is influenced by iodine deficiency (ID) and radiation exposure. Since radiation and ID can separately induce oxidative stress (OS) and microvascular responses in breast, their combination could additively increase these responses. Therefore, ID was induced in MCF7 and MCF12A breast cell lines by medium change. Read More

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Does Motor Cortex Engagement During Movement Preparation Differentially Inhibit Nociceptive Processing in Patients with Chronic Whiplash Associated Disorders, Chronic Fatigue Syndrome and Healthy Controls? An Experimental Study.

J Clin Med 2020 May 18;9(5). Epub 2020 May 18.

Pain in Motion International Research Group, www.paininmotion.be, Brussels, Belgium.

Background: Patients with chronic fatigue syndrome (CFS) and chronic whiplash associated disorders (cWAD) present a reduced ability to activate central descending nociceptive inhibition after exercise, compared to measurements before exercise. It was hypothesised that a dysfunctional motor-induced inhibition of nociception partly explains this dysfunctional exercise-induced hypoalgesia. This study investigates if engagement of the motor system during movement preparation inhibits nociception-evoked brain responses in these patients as compared to healthy controls (HC). Read More

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Identifying optimal level-of-care placement decisions for adolescent substance use treatment.

Drug Alcohol Depend 2020 07 28;212:107991. Epub 2020 Apr 28.

RAND Corporation, 1200 South Hayes Street, Arlington, VA 22202-5050, USA.

Background: Adolescents respond differentially to substance use treatment based on their individual needs and goals. Providers may benefit from guidance (via decision rules) for personalizing aspects of treatment, such as level-of-care (LOC) placements, like choosing between outpatient or inpatient care. The field lacks an empirically-supported foundation to inform the development of an adaptive LOC-placement protocol. Read More

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Targeting aberrant DNA hypermethylation as a driver of ATL leukemogenesis by using the new oral demethylating agent OR-2100.

Blood 2020 08;136(7):871-884

Department of Clinical Laboratory Medicine, Faculty of Medicine, and.

Adult T-cell leukemia-lymphoma (ATL) is an aggressive hematological malignancy of CD4+ T cells transformed by human T-cell lymphotropic virus-1 (HTLV-1). Most HTLV-1-infected individuals are asymptomatic, and only 3% to 5% of carriers develop ATL. Here, we describe the contribution of aberrant DNA methylation to ATL leukemogenesis. Read More

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Unique microRNA alterations in hepatocellular carcinomas arising either spontaneously or due to chronic exposure to Ginkgo biloba extract (GBE) in B6C3F1/N mice.

Arch Toxicol 2020 07 18;94(7):2523-2541. Epub 2020 Apr 18.

Cellular and Molecular Pathology Branch, DNTP, NIEHS, Research Triangle Park, NC, 27709, USA.

Ginkgo biloba extract (GBE) is used in traditional Chinese medicine as a herbal supplement for improving memory. Exposure of B6C3F1/N mice to GBE in a 2-year National Toxicology Program (NTP) bioassay resulted in a dose-dependent increase in hepatocellular carcinomas (HCC). To identify key microRNAs that modulate GBE-induced hepatocarcinogenesis, we compared the global miRNA expression profiles in GBE-exposed HCC (GBE-HCC) and spontaneous HCC (SPNT-HCC) with age-matched vehicle control normal livers (CNTL) from B6C3F1/N mice. Read More

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Alterations in the methylome of the stromal tumour microenvironment signal the presence and severity of prostate cancer.

Clin Epigenetics 2020 03 18;12(1):48. Epub 2020 Mar 18.

Epigenetics Research Laboratory, Genomics and Epigenetics Theme, Garvan Institute of Medical Research, 384 Victoria St, Darlinghurst, Sydney, NSW, 2010, Australia.

Background: Prostate cancer changes the phenotype of cells within the stromal microenvironment, including fibroblasts, which in turn promote tumour progression. Functional changes in prostate cancer-associated fibroblasts (CAFs) coincide with alterations in DNA methylation levels at loci-specific regulatory regions. Yet, it is not clear how these methylation changes compare across CAFs from different patients. Read More

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Iridium-Catalyzed Enantioselective and Diastereoselective Hydrogenation of 1,3-Disubstituted Isoquinolines.

ACS Catal 2020 Mar 10;10(5):3241-3248. Epub 2020 Feb 10.

Warren and Katharine Schlinger Laboratory for Chemistry and Chemical Engineering, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, United States.

The development of a general method utilizing a hydroxymethyl directing group for asymmetric hydrogenation of 1,3-disubstituted isoquinolines to provide chiral 1,2,3,4-tetrahydroisoquinolines is reported. The reaction, which utilizes [Ir(cod)Cl] and a commercially available chiral xyliphos ligand, proceeds in good yield with high levels of enantioselectivity and diastereo-selectivity (up to 95% ee and >20:1 dr) on a range of differentially substituted isoquinolines. Directing group studies demonstrate that the hydroxymethyl functional group at the C1-position is more efficient at enabling hydrogenation than other substituents, although high levels of enantioselectivity were conserved across a variety of polar and non-polar functional groups. Read More

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Dysregulated lncRNA-miRNA-mRNA Network Reveals Patient Survival-Associated Modules and RNA Binding Proteins in Invasive Breast Carcinoma.

Front Genet 2019 15;10:1284. Epub 2020 Jan 15.

Institute for Diabetes, Obesity, and Metabolism, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, United States.

Breast cancer is the most common cancer in women, but few biomarkers are effective in clinic. Previous studies have shown the important roles of non-coding RNAs in diagnosis, prognosis, and therapy selection for breast cancer and have suggested the significance of integrating molecules at different levels to interpret the mechanism of breast cancer. Here, we collected transcriptome data including long non-coding RNA (lncRNA), microRNA (miRNA), and mRNA for ~1,200 samples, including 1079 invasive breast carcinoma samples and 104 normal samples, from The Cancer Genome Atlas (TCGA) project. Read More

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January 2020

Gene expression changes in arterial and venous endothelial cells exposed to gestational diabetes mellitus.

Gynecol Endocrinol 2020 Sep 20;36(9):791-795. Epub 2020 Jan 20.

Department of Obstetrics and Gynecology, People's Hospital of Jiaxiang County, Jining, China.

We investigated the molecular changes in fetoplacental blood vessel endothelial cells in gestational diabetes mellitus (GDM). Raw gene expression profile data of arterial and venous endothelial cells from GDM complicated pregnancies and healthy controls were downloaded and used for bioinformatic analysis. There were two differentially expressed genes (DEGs) in venous endothelial cells and 178 DEGs in arterial endothelial cells induced by GDM. Read More

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September 2020