1,235 results match your criteria 109 colony

Recombinant Lactobacillus plantarum NC8 strain expressing porcine rotavirus VP7 induces specific antibodies in BALB/c mice.

Acta Biochim Biophys Sin (Shanghai) 2021 May 8. Epub 2021 May 8.

College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun 130118, China.

The major etiologic agent that causes acute gastroenteritis worldwide in young animals and children is Group A rotavirus. Currently, commercially available vaccines do not often prevent porcine rotavirus (PRV) infection. In this study, we evaluated the efficacy of oral recombinant Lactobacillus vaccine against PRV in a mouse model. Read More

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First Report of Leaf Spots caused by Nigrospora oryzae on Wild Rice in China.

Plant Dis 2021 Apr 26. Epub 2021 Apr 26.

Guangdong Ocean University, 74780, Zhanjiang, Guangdong, China;

In recent years, wild rice (Oryza rufipogon Griff) has been widely cultivated because of its health-promoting effects. In May 2019, leaf spot lesions on cv. Haihong-12 were observed in Zhanjiang (20. Read More

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Circular RNA hsa_circ_0000700 promotes cell proliferation and migration in Esophageal Squamous Cell Carcinoma by sponging miR-1229.

J Cancer 2021 5;12(9):2610-2623. Epub 2021 Mar 5.

Department of Radiotherapy, The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang, 310022, China.

Accumulating evidence has demonstrated that circular RNAs (circRNAs) are involved in the pathogenesis of cancer, including that of esophageal squamous cell carcinoma (ESCC). The current study aimed to investigate the role of hsa_circ_0000700 in ESCC. hsa_circ_0000700, miR-1229, and related functional gene expression was measured by RT-qPCR. Read More

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Tolerant Small-colony Variants Form Prior to Resistance Within a Staphylococcus aureus Biofilm Based on Antibiotic Selective Pressure.

Clin Orthop Relat Res 2021 04 7. Epub 2021 Apr 7.

R. Manasherob, D. W. Lowenberg, D. F. Amanatullah, Department of Orthopaedic Surgery, Stanford Medicine, Redwood City, CA, USA.

Background: The treatment of periprosthetic joint infection (PJI) is focused on the surgical or chemical removal of biofilm. Antibiotics in isolation are typically ineffective against PJI. Bacteria survive after antibiotic administration because of antibiotic tolerance, resistance, and persistence that arise in the resident bacteria of a biofilm. Read More

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Effects of a Lactobacilli Probiotic on Reducing Duration of URTI and Fever, and Use of URTI-Associated Medicine: A Re-Analysis of a Randomized, Placebo-Controlled Study.

Microorganisms 2021 Mar 4;9(3). Epub 2021 Mar 4.

Department of Bioprocess Technology, School of Industrial Technology, University Sains Malaysia, Penang 11800, Malaysia.

We previously reported on the effects of DR7 on reducing Upper Respiratory Tract Infections (URTI) symptoms' score and frequency in 109 adults upon a 12-week consumption at 10 colony-forming units (CFU)/day, but several limitations were detected in the publication. Thus, the present study re-analyzed some data with the aim to address some of these weaknesses, and presents new data on duration of URTI and consumption of URTI-associated medication, as compared to the placebo. Our re-analyses found probiotic administration significantly reduced the proportion of patient days of URTI and of fever (all < 0. Read More

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First Report of Macrophomina phaseolina Causing Charcoal Rot of Peanut (Arachis hypogaea L.) in Mexico.

Plant Dis 2021 Mar 23. Epub 2021 Mar 23.

Benemerita Universidad Autonoma de Puebla, 3972, Centro de Agroecología, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla Edificio VAL 1, Km 1.7 carretera a San Baltazar Tetela, Zacachimalpa, Pueba, Puebla, Puebla, Mexico, 72960;

Peanut (Arachis hypogaea L.) is the third most important oilseed crop in the world. The cultivated area in Mexico is currently 52,046 ha with a production of 91,109 ton in 2018 (FAO, 2020). Read More

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Naturally occurring aesculetin coumarin exerts antiproliferative effects in gastric cancer cells mediated via apoptotic cell death, cell cycle arrest and targeting PI3K/AKT/M-TOR signalling pathway.

Acta Biochim Pol 2021 Mar;68(1):109-113

1Department of General Surgery, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing, China; 2Department of General Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

Aesculetin is an active member of coumarins that has been reported to possess significant medicinal and biological importance. It has also been shown with potential anticancer activity against different human cancers including breast, lung and hepatocellular carcinoma. Therefore, the current investigation was undertaken to examine the anticancer effects of aesculetin against gastric cancer. Read More

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IL-32 Promotes the Radiosensitivity of Esophageal Squamous Cell Carcinoma Cell through STAT3 Pathway.

Biomed Res Int 2021 17;2021:6653747. Epub 2021 Feb 17.

Department of Radiotherapy, Lianyungang Municipal Oriental Hospital Affiliated to Bengbu Medical Collage, Lianyungang, 222042 Jiangsu, China.

Objective: This study is set out to determine the relationship between IL-32 and radiosensitivity of esophageal squamous cell carcinoma (ESCC).

Methods: Western blot was adopted for measuring IL-32 expression in Eca-109 and TE-10 cells. Eca-109 and TE-10 cells with interference or overexpression of IL-32 were treated with the presence or absence of X-ray irradiation. Read More

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February 2021

Prognostic impact of GPR56 in patients with colorectal cancer.

Neoplasma 2021 Feb 24. Epub 2021 Feb 24.

Division of Colon and Rectal Surgery, Department of Surgery, Yonsei University College of Medicine, Yonsei University Severance Hospital, Seoul, South Korea.

G protein-coupled receptor 56 (GPR56) belongs to the adhesion G protein-coupled receptor subfamily, which plays a role in cell progression and survival. The aim of this study was to investigate the role of the GPR56 gene in a cell line study and the impact of its protein expression on the prognosis of colorectal cancer (CRC) patients. The effect of GPR56 on tumor cell proliferation (WST-1 assay), invasion (Transwell assay), migration (Transwell assay, wound healing assay), and colony-forming ability (semisolid agar colony-forming assay) was explored. Read More

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February 2021

STAT3-induced HLA-F-AS1 promotes cell proliferation and stemness characteristics in triple negative breast cancer cells by upregulating TRABD.

Bioorg Chem 2021 Apr 10;109:104722. Epub 2021 Feb 10.

Department of Breast Surgery, The First Hospital of Jilin University, Changchun 130021, Jilin, China. Electronic address:

Breast cancer (BC) is one of the most common malignances and is a leading cause of cancer-related deaths in women globally. Triple negative breast cancer (TNBC) is a common subtype of BC. Emerging evidence has indicated the crucial roles of long noncoding RNAs (lncRNAs) in the tumorigenesis of TNBC. Read More

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Discovery of higenamine as a potent, selective and cellular active natural LSD1 inhibitor for MLL-rearranged leukemia therapy.

Bioorg Chem 2021 Apr 10;109:104723. Epub 2021 Feb 10.

Joint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education of the People's Republic of China, International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, China. Electronic address:

Natural products are a rich source of lead compounds and have shown promise for epigenetic drug discovery. In this work, we discovered higenamine from our natural product library as a potent, selective and cellular active natural LSD1 inhibitor. Higenamine shows acceptable potency against LSD1 and high selectivity towards LSD1 over MAOA/B. Read More

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Respiratory and Reproductive Impairment of Commercial Layer Chickens After Experimental Infection with Gallibacterium anatis Biovar haemolytica.

Avian Dis 2020 12;64(4):536-541

Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Alexandria University, Edfina, 22758 Egypt.

The prevalence of Gallibacterium anatis in poultry production has increased over the last two decades. However, only a few studies have explored the pathogenicity of this bacterium in commercial layer chickens. This trial studied the aspects of the pathogenicity of a Gallibacterium anatis biovar haemolytica local Egyptian isolate (previously registered as strain B14 with GenBank accession no. Read More

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December 2020

Detection of and Genes in Isolated from Clinical Specimens at Tertiary Care Heart Hospital, Kathmandu, Nepal.

Diseases 2021 Feb 7;9(1). Epub 2021 Feb 7.

Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu 44618, Nepal.

Background: Antimicrobial resistance (AMR) among Gram-negative pathogens, predominantly ESBL-producing clinical isolates, are increasing worldwide. The main aim of this study was to determine the prevalence of ESBL-producing clinical isolates, their antibiogram, and the frequency of ESBL genes ( and ) in the clinical samples from patients.

Methods: A total of 1065 clinical specimens from patients suspected of heart infections were collected between February and August 2019. Read More

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February 2021

The use of live yeast to increase intake and performance of cattle receiving low-quality tropical forages.

J Anim Sci 2021 Feb;99(2)

Queensland Alliance for Agriculture and Food Innovation, Centre for Animal Science, The University of Queensland, St Lucia, QLD, Australia.

The objective was to evaluate the effects of a specific strain of live yeast (LY) on growth performance, fermentation parameters, feed efficiency, and bacterial communities in the rumen of growing cattle fed low-quality hay. In experiment (exp.) 1, 12 Droughtmaster bull calves (270 ± 7. Read More

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February 2021

Discovery of novel indole derivatives that inhibit NEDDylation and MAPK pathways against gastric cancer MGC803 cells.

Bioorg Chem 2021 Feb 8;107:104634. Epub 2021 Jan 8.

School of Basic Medical Science, Zhengzhou University, Zhengzhou 450001, China; The Academy of Medical Science, Zhengzhou University, Zhengzhou 450001, China; Henan Institute of Advanced Technology, Zhengzhou University, Zhengzhou 450001, China; State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210023, Jiangsu, China. Electronic address:

A series of novel indole derivatives were synthesized and evaluated for their antiproliferative activity against three selected cancer cell lines (MGC803, EC-109 and PC-3). Among these analogues, 2-(5-methoxy-1H-indol-1-yl)-N-(4-methoxybenzyl)-N-(3,4,5-trimethoxyphenyl)acetamide (V7) showed the best inhibitory activity against MGC803 cells with an IC value of 1.59 μM. Read More

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February 2021

Surveillance of disease incidence in shrimp farms located in the east coastal region of India and in vitro antibacterial efficacy of probiotics against Vibrio parahaemolyticus.

J Invertebr Pathol 2021 Feb 17;179:107536. Epub 2021 Jan 17.

Disease Control and Prevention Lab, Department of Animal Health and Management, Alagappa University, Science Campus, Karaikudi 630 003, India. Electronic address:

We surveyed 130 shrimp farms located on the eastern coast of India to determine the prevalence of emerging diseases in Litopenaeus vannamei and Penaeus monodon. Live shrimps were collected from the farms based on external symptoms. The biochemical, molecular, and histopathology results confirmed infection with Enterocytozoon hepatopenaei (32. Read More

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February 2021

[Hydrogen plays a protective role in intestinal injury of mice with severe sepsis by regulating the release of heme oxygenase-1 and high mobility group protein B1].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2020 Nov;32(11):1372-1377

Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin 300052, China. Corresponding author: Yu Yonghao, Email:

Objective: To investigate the protective effect of the hydrogen (H) inhalation on intestinal injury in severe sepsis mice and its relationship with nuclear factor E2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1)/high mobility group protein B1 (HMGB1) pathway.

Methods: The 280 male wild-type (WT) and Nrf2 knockout (Nrf2-KO) ICR mice (6-8 weeks old, 20-25 g weight) were assigned into 8 groups randomly (WT and Nrf2-KO mice, 4 groups respectively, n = 35): Sham group, H inhalation group (Sham+H group), sepsis model group which was induced by cecal ligation and puncture (CLP group) and H treatment group (CLP+H group). In Sham group, except cecal ligation and perforation, the same surgery was used. Read More

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November 2020

High throughput instrument to screen fluorescent proteins under two-photon excitation.

Biomed Opt Express 2020 Dec 17;11(12):7192-7203. Epub 2020 Nov 17.

Department of Cell Biology & Neuroscience, Montana State University, 109 Lewis Hall, Bozeman, MT 59717, USA.

Two-photon microscopy together with fluorescent proteins and fluorescent protein-based biosensors are commonly used tools in neuroscience. To enhance their experimental scope, it is important to optimize fluorescent proteins for two-photon excitation. Directed evolution of fluorescent proteins under one-photon excitation is common, but many one-photon properties do not correlate with two-photon properties. Read More

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December 2020

Translocating Mycobacterium ulcerans: An experimental model.

PLoS One 2020 30;15(12):e0230544. Epub 2020 Dec 30.

Aix-Marseille Univ., IRD, MEPHI, IHU Méditerranée Infection, Marseille, France.

Mycobacterium ulcerans is a non-tuberculous environmental mycobacterium responsible for extensive cutaneous and subcutaneous ulcers in mammals, known as Buruli ulcer in humans. M. ulcerans has seldom been detected in the faeces of mammals and has not been detected in human faeces. Read More

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January 2021

Lactobacillus attenuates progression of nonalcoholic fatty liver disease by lowering cholesterol and steatosis.

Clin Mol Hepatol 2021 01 3;27(1):110-124. Epub 2020 Dec 3.

Institue for Liver and Digestive Diseases, Hallym University College of Medicine, Chuncheon, Korea.

Background/aims: Nonalcoholic fatty liver disease (NAFLD) is closely related to gut-microbiome. There is a paucity of research on which strains of gut microbiota affect the progression of NAFLD. This study explored the NAFLD-associated microbiome in humans and the role of Lactobacillus in the progression of NAFLD in mice. Read More

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January 2021

Refinement of the CS6-expressing enterotoxigenic Escherichia coli strain B7A human challenge model: A randomized trial.

PLoS One 2020 2;15(12):e0239888. Epub 2020 Dec 2.

Naval Medical Research Center, Silver Spring, MD, United States of America.

Background: Human challenge models for enterotoxigenic Escherichia coli (ETEC) facilitate vaccine down-selection. The B7A (O148:H28 CS6+LT+ST+) strain is important for vaccine development. We sought to refine the B7A model by identifying a dose and fasting regimen consistently inducing moderate-severe diarrhea. Read More

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January 2021

Comparison of the Pathogenicity of Five Clostridium perfringens Isolates Using an Eimeria maxima Coinfection Necrotic Enteritis Disease Model in Commercial Broiler Chickens.

Avian Dis 2020 09;64(3):386-392

Animal Bioscience and Biotechnology Laboratory, Agricultural Research Service (ARS), United States Department of Agriculture (USDA), Beltsville, MD 20705.

Clostridium perfringens (CP) is the etiologic agent of necrotic enteritis (NE) in broiler chickens that is responsible for massive economic losses in the poultry industry in response to voluntary reduction and withdrawal of antibiotic growth promoters. Large variations exist in the CP isolates in inducing intestinal NE lesions. However, limited information is available on CP isolate genetics in inducing NE with other predisposing factors. Read More

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September 2020

Peri-implant treatment reduces the salivary levels of Colony stimulator factor-1 and S100A8/A9.

Odontology 2021 Apr 13;109(2):540-546. Epub 2020 Nov 13.

School of Dentistry and Oral Health and Menzies Health Institute Queensland, Griffith University Queensland, Gold Coast campus Parklands Drive, Southport, QLD, 4222, Australia.

This study evaluated the impact of peri-implant treatment in the salivary levels of Colony stimulator factor -1 (CSF-1), S100A8/A9 and S100A12 in patients having mucositis or peri-implantitis. As a secondary aim, we analysed the correlation between the salivary and peri-implant crevicular fluid (PICF) levels. Forty-seven patient, 27 having mucositis (mean age 63. Read More

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Effect of Five Commercial Probiotic Formulations on Candida Albicans Growth: In Vitro Study.

J Clin Pediatr Dent 2020 Sep;44(5):315-322

Purpose: The objective was to evaluate the antagonistic effect of Lactobacillus and Bifidobacterium recovered from five commercial probiotics on the growth of C. albicans.

Study Design: The Lactobacillus and Bifidobacterium strains of five commercial probiotics were recovered and grown: Probio Hp+®, ProBiseis®, Lactipan®, Liolactil®, and Lacteol Fort®; 50 mg of each was hydrated and grown in Lactobacilli MRS (De Man, Rogosa and Sharpe) broth and incubated at 37°C with stirring (120 RPM) for 24 hours. Read More

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September 2020

Downregulation of Ubiquitin Inhibits the Aggressive Phenotypes of Esophageal Squamous Cell Carcinoma.

Technol Cancer Res Treat 2020 Jan-Dec;19:1533033820973282

School of Radiation Medicine and Protection and State Key Laboratory of Radiation Medicine and Protection, 74565Medical College of Soochow University, Suzhou, People's Republic of China.

Purpose: Esophageal cancer is one of the most common malignancies worldwide. Ubiquitin-dependent degradation of regulatory proteins reportedly plays a central role in diverse cellular processes. This study investigated the expression levels of ubiquitin in esophageal squamous cell carcinoma tissues and the functions of ubiquitin in the context of esophageal squamous cell carcinoma progression. Read More

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November 2020

Chitosan/zinc nitrate microneedles for bacterial biofilm eradication.

J Biomed Mater Res B Appl Biomater 2021 Jun 5;109(6):911-920. Epub 2020 Nov 5.

School of Electro-mechanical Engineering, Guangdong University of Technology, Guangzhou, China.

Chronic wounds are greatly health threatening owing to the increasing morbidity, and bacterial biofilm is a major cause of chronic wounds. The critical for bacterial biofilm eradication is overcome the barrier of extracellular polymeric substances (EPS) produced by the bacteria, and promote the diffusion of drugs within the biofilm. In this article, composite microneedles (MNs) of chitosan and zinc nitrate (CS-Zn[II] MNs) were investigated to eradicate bacterial biofilm. Read More

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Four new species of in the clade from northern China.

MycoKeys 2020 8;73:109-132. Epub 2020 Oct 8.

State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 3 Park 1, Beichen West Road, Chaoyang District, Beijing 100101, China Chinese Academy of Sciences Beijing China.

The clade of comprises many species, which are associated with a wide variety of substrates. In this study, four new species of , namely , , , and , were encountered from a fruiting body and compost of , soil, and vermicompost. Their colony and mycelial morphology, including features of asexual states, were described. Read More

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October 2020

Interventions to reduce contaminated aerosols produced during dental procedures for preventing infectious diseases.

Cochrane Database Syst Rev 2020 10 12;10:CD013686. Epub 2020 Oct 12.

Cochrane Work, Department of Public and Occupational Health, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands.

Background: Many dental procedures produce aerosols (droplets, droplet nuclei and splatter) that harbour various pathogenic micro-organisms and may pose a risk for the spread of infections between dentist and patient. The COVID-19 pandemic has led to greater concern about this risk.

Objectives: To assess the effectiveness of methods used during dental treatment procedures to minimize aerosol production and reduce or neutralize contamination in aerosols. Read More

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October 2020

MicroRNA (MiR)-301a-3p regulates the proliferation of esophageal squamous cells via targeting PTEN.

Bioengineered 2020 12;11(1):972-983

Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University , Shijiazhuang, China.

Human esophageal carcinoma (EC) is a common cancer, which leads to many deaths worldwide every year. Our study aimed to explore the mechanism of miR-301a-3p regulating the proliferation of esophageal squamous cell carcinoma (ESCC) cells. We had collected ESCC tissues and adjacent normal esophageal tissues from 47 patients. Read More

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December 2020

Quality of life in patients with metastatic prostate cancer following treatment with cabazitaxel versus abiraterone or enzalutamide (CARD): an analysis of a randomised, multicentre, open-label, phase 4 study.

Lancet Oncol 2020 11 11;21(11):1513-1525. Epub 2020 Sep 11.

Erasmus Medical Center, Rotterdam, Netherlands.

Background: In the CARD study, cabazitaxel significantly improved radiographic progression-free survival and overall survival versus abiraterone or enzalutamide in patients with metastatic castration-resistant prostate cancer previously treated with docetaxel and the alternative androgen signalling-targeted inhibitor. Here, we report the quality-of-life outcomes from the CARD study.

Methods: CARD was a randomised, multicentre, open-label, phase 4 study involving 62 clinical sites across 13 European countries. Read More

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November 2020