6 results match your criteria 100 µm prevented

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Immobilization of foreign protein in BmNPV polyhedra by fusion expression with partial polyhedrin fragments.

J Virol Methods 2013 Dec 2;194(1-2):185-9. Epub 2013 Sep 2.

Lab of Silkworm Biotechnology, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

Bombyx mori nucleopolyhedrovirus (BmNPV) produces large, proteinaceous crystal matrix named polyhedra, which occlude progeny virions which are produced during infection and protect virions from hostile environmental conditions. In this study, five overlapping N-terminal fragments of the BmNPV polyhedrin ORF were cloned and ligated with the foreign gene egfp, and five recombinant baculoviruses were constructed by BmNPV(Polh(+)) Bac-to-Bac baculovirus expression system was used to co-express the polyhedrin and fused protein. The results showed that the fusion proteins were highly expressed, and the foreign proteins fused with the 100aa fragment of polyhedrin could be embedded into polyhedra at a higher ratio. Read More

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December 2013

[Functional analysis of the late expression factor genes of plutella xylostella granulovirus].

Bing Du Xue Bao 2012 Sep;28(5):560-6

Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China.

Plutella xylostella granulovirus (PlxyGV) contains homologs of 15 Autographa californica MNPV (AcMNPV) late expression factor (lef) genes. The prospective products of 14 PlxyGV lef genes (ie-0 is not included) share 13%-53% amino acid similarity with their corresponding homologs of AcMNPV, among which LEF-9, LEF-8 and P47, three subunits of the virus-encoded RNA polymerase, share 49%, 53% and 46% sequence identity, respectively. In this study, an established transient expression system was used to test the ability of the PlxyGV LEFs to activate an AcMNPV vp39 promoter-driven reporter gene in SF9 cells. Read More

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September 2012

Mapping of conformational autoantibody epitopes in ZNT8.

Diabetes Metab Res Rev 2011 Nov;27(8):883-6

Barbara Davis Center for Childhood Diabetes, University of Colorado Denver, Aurora, CO 80045, USA.

Background: Identification of the major humoral epitopes in zinc transporter 8 (ZnT8) will expand the range of biomarkers for human type 1 diabetes and may provide clues to the mechanisms governing disease progression. Our initial studies suggested that most ZnT8-reactive sera recognize conformational epitopes in the final 100aa region of the molecule. Subsequently we identified residue 325 as a major determinant in two epitopes linked to a genetic polymorphism with high minor allele frequency (rs13266634). Read More

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November 2011

Evidence for a fourteenth mtDNA-encoded protein in the female-transmitted mtDNA of marine Mussels (Bivalvia: Mytilidae).

PLoS One 2011 Apr 27;6(4):e19365. Epub 2011 Apr 27.

Kent State University, Kent, Ohio, United States of America.

Background: A novel feature for animal mitochondrial genomes has been recently established: i.e., the presence of additional, lineage-specific, mtDNA-encoded proteins with functional significance. Read More

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Characterization of Choristoneura fumiferana genes of the sixth subunit of the origin recognition complex: CfORC6.

J Biochem Mol Biol 2006 Nov;39(6):782-7

College of Life Science, Henan Agricultural University, Zhengzhou, China, 45002.

A new protein was cloned and identified as the sixth subunit of Choristoneura fumiferana origin recognition complex (CfORC6). The newly identified 43 kDa protein CfORC6 is much bigger than DmORC6 (25.7 kDa) and HsORC6 (28. Read More

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November 2006

A comparison between the nutritive value of short-cutting cycle, high temperature-dried alfalfa and timothy hay for horses.

Equine Vet J 1992 Mar;24(2):84-9

Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, Midlothian, UK.

The objective was to evaluate the nutritive value of short-cutting cycle, high temperature-dried (SCCHTD) alfalfa compared to timothy hay. This was achieved by carrying out 4 x 4 Latin Square digestibility trial using 4 Thoroughbred (one three-quarter Thoroughbred) horses (mean liveweight, 531 kg). The four dietary treatments were 0AA (timothy hay only), 33AA (0. Read More

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