Plant Dis 2021 Nov 7:PDIS12202695RE. Epub 2021 Nov 7.
Occupational and Environmental Health, Dalla Lana School of Public Health, University of Toronto, Toronto, Ontario M5T 1R4, Canada.
Quantitative PCR (qPCR), loop-mediated amplification (LAMP), and lateral flow strip-based recombinase polymerase amplification (RPA-LFS) assays were assessed for early detection of , the global causal agent of potato and tomato late blight, on passive wind-powered spore traps known as Spornados. Spore traps were deployed in potato and tomato fields during the 2018, 2019, and 2020 growing seasons in the provinces of Alberta, British Columbia, Manitoba, Prince Edward Island, and Ontario. All assays used DNA extracts from Spornado cassette membranes targeting the nuclear ribosomal internal transcribed spacer. Read More