4,536 results match your criteria 003 pcr


The role of tumor acidification in aggressiveness, cell dissemination and treatment resistance of oral squamous cell carcinoma.

Life Sci 2021 Nov 22:120163. Epub 2021 Nov 22.

Oral Pathology Department, School of Dentistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 90035-003, Brazil.; Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS 90035-903, Brazil.. Electronic address:

Aims: To investigate the role of tumor acidification in cell behavior, migration, and treatment resistance of oral squamous cell carcinoma (OSCC).

Main Methods: The SCC4 and SCC25 cell lines were exposed to acidified (pH 6.8) cell culture medium for 7 days. Read More

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November 2021

A microenvironment of high lactate and low pH created by the blastocyst promotes endometrial receptivity and implantation.

Reprod Biomed Online 2021 Sep 20. Epub 2021 Sep 20.

School of BioSciences, University of Melbourne VIC 3010, Australia; Melbourne IVF, East Melbourne VIC 3002, Australia. Electronic address:

Research Question: Is the blastocyst's idiosyncratic metabolic production of lactate, and creation of a specialized microenvironment at the implatation site, an important mediator of maternal-fetal signalling to promote endometrial receptivity and implantation?

Design: Hormonally primed ECC-1 and Ishikawa cells were used to assess functional changes to the endometrial epithelium after exposure to lactic acid (LA), LA with neutralized pH (nLA) or acidic pH (pH). Tight junction integrity (transepithelial resistance [TER]), cellular proliferation or changes to gene expression by RT-PCR were analysed. The effect of LA on Endometrial stromal cells decidualization and migratory capacity, and HUVEC endothelial tube formation and angiogenesis, were also assessed. Read More

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September 2021

Genome-Wide Identification of Circular RNAs Potentially Involved in the Biosynthesis of Secondary Metabolites in .

Front Genet 2021 5;12:645115. Epub 2021 Nov 5.

Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine from Ministry of Education, Engineering Research Center of Chinese Medicine Resources from Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.

Circular RNAs (circRNAs) play various roles in cellular functions. However, no studies have been reported on the potential involvement of circRNAs in the biosynthesis of secondary metabolites in plants. Here, we performed a genome-wide discovery of circRNAs from root, stem and leaf samples of using RNA-Seq. Read More

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November 2021

Prevalence of camel babesiosis in southeast of Iran.

Vet Med Sci 2021 Nov 20. Epub 2021 Nov 20.

Vector-Borne Diseases Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran.

Babesiosis is a globally distributed zoonotic parasitic disease in a broad range of vertebrates with great importance in the veterinary field. The standard diagnostic test for Babesiosis in animals is microscopic identification of the parasite in a venous blood smear stained with Giemsa combined with assessment of clinical manifestations throughout the acute phase of the disease. The present study was planned to determine the presence of Babesia species in camels from the southeastern regions of Iran. Read More

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November 2021

Association of a common genetic variant (insertion/deletion) in ACE gene with prostate cancer susceptibility in a Tunisian population.

J Clin Lab Anal 2021 Nov 19:e24129. Epub 2021 Nov 19.

Laboratory of Protein Engineering and Bio-active Molecules, National Institute of Applied Science and Technology - University of Carthage, Tunis, Tunisia.

Background: Angiotensin-converting enzyme (ACE) plays a pivotal role in several pathologies including cancers. The association of insertion/deletion (I/D) polymorphism of the ACE gene with prostate cancer (PC) risk remains controversial. We aimed to investigate for the first time, to our Knowledge, in North Africa the potential relationship between ACE I/D polymorphism with PC susceptibility and clinical outcomes of PC patients. Read More

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November 2021

Associations of streptococci and fungi amounts in the oral cavity with nutritional and oral health status in institutionalized elders: a cross sectional study.

BMC Oral Health 2021 Nov 19;21(1):590. Epub 2021 Nov 19.

Division of Preventive Dentistry, Department of Oral Medicine, Iwate Medical University School of Dentistry, 1-3-27 Chuo-dori, Morioka, Iwate, 020-8505, Japan.

Background: Disruption of the indigenous microbiota is likely related to frailty caused by undernutrition. However, the relationship between undernutrition and the oral microbiota, especially normal bacteria, is not obvious. The aim of this study was to elucidate the associations of nutritional and oral health conditions with prevalence of bacteria and fungi in the oral cavity of older individuals. Read More

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November 2021

Expression characteristic of and in acute myeloid leukemia.

Bioengineered 2021 Nov 17. Epub 2021 Nov 17.

School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.

isoforms, () and () expression characteristics in acute myeloid leukemia (AML) remain unknown. This study investigated mRNA and membrane protein expression of two isoforms in AML cell lines and de novo patients by using RT-PCR and flow cytometry, and analyzed the promoter methylation state by utilizing RQ-MSP. was the dominant isoform. Read More

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November 2021

Comparing Biomarkers for Predicting Pathological Responses to Neoadjuvant Therapy in HER2-Positive Breast Cancer: A Systematic Review and Meta-Analysis.

Front Oncol 2021 28;11:731148. Epub 2021 Oct 28.

Breast Disease Diagnosis and Treatment Center of Affiliated Hospital of Qinghai University & Affiliated Cancer Hospital of Qinghai University, Xining, China.

Introduction: The predictive strength and accuracy of some biomarkers for the pathological complete response (pCR) to neoadjuvant therapy for HER2-positive breast cancer remain unclear. This study aimed to compare the accuracy of the HER2-enriched subtype and the presence of PIK3CA mutations, namely, TILs, HRs, and Ki-67, in predicting the pCR to HER2-positive breast cancer therapy.

Methods: We screened studies that included pCR predicted by one of the following biomarkers: the HER2-enriched subtype and the presence of PIK3CA mutations, TILs, HRs, or Ki-67. Read More

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October 2021

Long-term clinical and biochemical residue after COVID-19 recovery.

Egypt Liver J 2021 12;11(1):74. Epub 2021 Sep 12.

Gastroenterology and Hepatology Unit, Internal Medicine Department, Faculty of Medicine, Mansoura University, Mansoura, Dakahlia Egypt.

Background: The long-term health consequences of coronavirus disease 2019 (COVID-19) are still unclear. The majority of previous trials addressed the post-COVID-19 symptoms through comprehensive medical questionnaires for relatively short periods after recovery. We tried to detect the potential pathological clinical signs and biochemical residue which persist for more than 3 months after the negative real-time polymerase chain reaction (RT-PCR) test of SARS-CoV-2. Read More

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September 2021

Functional Activity of the Complement System in Hospitalized COVID-19 Patients: A Prospective Cohort Study.

Front Immunol 2021;12:765330. Epub 2021 Oct 28.

Division of Internal Medicine, University Hospital Basel, Basel, Switzerland.

Aims: Although the exact factors promoting disease progression in COVID-19 are not fully elucidated, unregulated activation of the complement system (CS) seems to play a crucial role in the pathogenesis of acute lung injury (ALI) induced by SARS-CoV-2. In particular, the lectin pathway (LP) has been implicated in previous autopsy studies. The primary purpose of our study is to investigate the role of the CS in hospitalized COVID-19 patients with varying degrees of disease severity. Read More

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November 2021

Expression of BCRP/ABCG2 Protein in Invasive Breast Cancer and Response to Neoadjuvant Chemotherapy.

Oncol Res Treat 2021 Nov 12. Epub 2021 Nov 12.

Background: Breast cancer resistance protein (BCRP), or ABCG2 (ATP-binding cassette sub-family G member 2), is an ATP-binding cassette (ABC) transporter that mediates energy-dependent transport of substrate drugs out of the cell. Its overexpression may contribute to intrinsic drug resistance in vitro. However, the current literature has not yet clarified the clinical significance of BCRP/ABCG2 in invasive breast carcinoma. Read More

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November 2021

Molecular Characterization of Peritoneal Involvement in Primary Colon and Ovary Neoplasm: The Possible Clinical Meaning of the P2X7 Receptor-Inflammasome Complex.

Eur Surg Res 2021 Nov 10:1-9. Epub 2021 Nov 10.

Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.

Introduction: Colon cancer (CC) and epithelial ovarian cancer (EOC) are common and severe neoplasms frequently sharing a massive inflammatory involvement of peritoneum. A detailed molecular characterization of such carcinomatosis has not been performed, so far.

Methods: Omental adipocytes were isolated from thirty-three adult women who underwent primary surgery for CC or EOC. Read More

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November 2021

The percentage of residual DCIS in patients diagnosed with primary invasive breast cancer treated with neoadjuvant systemic therapy: A nationwide retrospective study.

Eur J Surg Oncol 2021 Oct 22. Epub 2021 Oct 22.

Department of Radiology and Nuclear Medicine, Maastricht University Medical Centre+, 6202 AZ, Maastricht, the Netherlands. Electronic address:

Introduction: Neoadjuvant systemic therapy (NST) is increasingly applied in breast cancer to improve surgical and oncological outcome. Approximately 21% of patients receiving NST achieve pathological complete response (pCR) of the breast. There is disagreement on the definition of pCR with respect to residual DCIS (ypT0 versus ypT0/is). Read More

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October 2021

Characteristics and outcome profile of hospitalized African patients with COVID-19: The Ethiopian context.

PLoS One 2021 9;16(11):e0259454. Epub 2021 Nov 9.

Millennium COVID-19 Care Center, St. Paul's Hospital Millennium Medical College, Addis Ababa, Ethiopia.

Background: The COVID-19 pandemic seems to have a different picture in Africa; the first case was identified in the continent after it had already caused a significant loss to the rest of the world and the reported number of cases and mortality rate has been low. Understanding the characteristics and outcome of the pandemic in the African setup is therefore crucial.

Aim: To assess the characteristics and outcome of Patients with COVID-19 and to identify determinants of the disease outcome among patients admitted to Millennium COVID-19 Care Center in Ethiopia. Read More

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November 2021

Occurrence of pulmonary residuals as one of the sequelae of COVID-19 and it's predictors among moderate and severe cases.

Indian J Tuberc 2021 Oct 3;68(4):450-456. Epub 2021 Feb 3.

Department of Chest Diseases, Faculty of Medicine, Cairo University, Egypt.

Background: Moderate and severe COVID-19 patients typically present with pneumonia. In this study we aimed to detect the occurrence of pulmonary residuals as a late sequela of COVID-19 and to identify it's predictors among moderate and severe cases.

Methods: This observational prospective study involved 85 COVID-19 patients confirmed by real time polymerase chain reaction (RT-PCR) nasopharyngeal swab, patients were recruited in the period of 1 st of June to 1 st of July. Read More

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October 2021

Role of ACE Polymorphism in Acute Ischemic Stroke.

Neurol India 2021 Sep-Oct;69(5):1217-1221

Department of Neurology, Lady Hardinge Medical College and Associated Hospitals, Delhi, India.

Background: Stroke is one of the leading causes of death and disability in India. Angiotensin-converting enzyme (ACE) is involved in the development of hypertension, atherosclerosis, cardio, and cerebrovascular disease and is a plausible genetic candidate for stroke. The role of ACE polymorphism is still uncertain and requires further investigation. Read More

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November 2021

Long-term persistence of SARS-CoV-2 neutralizing antibody responses after infection and estimates of the duration of protection.

EClinicalMedicine 2021 Nov 30;41:101174. Epub 2021 Oct 30.

School of Public Health, The University of Hong Kong, Special Administrative Region of Hong Kong, China.

Background: The duration of immunity in SARS-CoV-2 infected people remains unclear. Neutralizing antibody responses are the best available correlate of protection against re-infection. Recent studies estimated that the correlate of 50% protection from re-infection was 20% of the mean convalescent neutralizing antibody titre. Read More

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November 2021

The Potential Regulatory Mechanism of lncRNA 122K13.12 and lncRNA 326C3.7 in Ankylosing Spondylitis.

Front Mol Biosci 2021 21;8:745441. Epub 2021 Oct 21.

Department of Rheumatology and Immunology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

This work aims to analyze and construct a novel competing endogenous RNA (ceRNA) network in ankylosing spondylitis (AS) with bone bridge formation, lncRNA. Using RNA sequencing and bioinformatics, we analyzed expression profiles of long noncoding RNAs (lncRNAs), microRNAs (miRNAs), and mRNAs in whole blood cells from 5 AS patients and 3 healthy individuals. Next, we verified the expression levels of candidate lncRNAs in 97 samples using the ΔΔCt value of real-time quantitative polymerase chain reaction (qRT-PCR). Read More

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October 2021

Assessing the Use of DNA Detection Platforms Combined With Passive Wind-Powered Spore Traps for Early Surveillance of Potato and Tomato Late Blight in Canada.

Plant Dis 2021 Nov 7:PDIS12202695RE. Epub 2021 Nov 7.

Occupational and Environmental Health, Dalla Lana School of Public Health, University of Toronto, Toronto, Ontario M5T 1R4, Canada.

Quantitative PCR (qPCR), loop-mediated amplification (LAMP), and lateral flow strip-based recombinase polymerase amplification (RPA-LFS) assays were assessed for early detection of , the global causal agent of potato and tomato late blight, on passive wind-powered spore traps known as Spornados. Spore traps were deployed in potato and tomato fields during the 2018, 2019, and 2020 growing seasons in the provinces of Alberta, British Columbia, Manitoba, Prince Edward Island, and Ontario. All assays used DNA extracts from Spornado cassette membranes targeting the nuclear ribosomal internal transcribed spacer. Read More

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November 2021

Molecular Signatures of Human Chronic Atrial Fibrillation in Primary Mitral Regurgitation.

Cardiovasc Ther 2021 15;2021:5516185. Epub 2021 Oct 15.

Stem Cell Institute, Ankara University, Ankara, Turkey.

Objectives: Transcriptomics of atrial fibrillation (AFib) in the setting of chronic primary mitral regurgitation (MR) remains to be characterized. We aimed to compare the gene expression profiles of patients with degenerative MR in AFib and sinus rhythm (SR) for a clearer picture of AFib pathophysiology.

Methods: After transcriptomic analysis and bioinformatics ( = 59), differentially expressed genes were defined using 1. Read More

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November 2021

Associations of plasma metal levels with type 2 diabetes and the mediating effects of microRNAs.

Environ Pollut 2022 Jan 1;292(Pt B):118452. Epub 2021 Nov 1.

Department of Occupational and Environmental Health and State Key Laboratory of Environmental Health for Incubating, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. Electronic address:

The present study aims to determine the associations of multiple plasma metal levels and plasma microRNAs (miRNAs) with diabetes risk, and further explore the mediating effects of plasma miRNAs on the associations of plasma metal with diabetes risk. We detected plasma levels of 23 metals by inductively coupled plasma mass spectrometry (ICP-MS) among 94 newly diagnosed and untreated diabetic cases and 94 healthy controls. The plasma miRNAs were examined by microRNA Array screening and Taqman real-time PCR validation among the same study population. Read More

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January 2022

Air Bubbles Activate Complement and Trigger Hemostasis and C3-Dependent Cytokine Release Ex Vivo in Human Whole Blood.

J Immunol 2021 12 3;207(11):2828-2840. Epub 2021 Nov 3.

Research Laboratory, Nordland Hospital Trust, Bodø, Norway.

Venous air embolism, which may complicate medical and surgical procedures, activates complement and triggers thromboinflammation. In lepirudin-anticoagulated human whole blood, we examined the effect of air bubbles on complement and its role in thromboinflammation. Whole blood from 16 donors was incubated with air bubbles without or with inhibitors of C3, C5, C5aR1, or CD14. Read More

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December 2021

The Use of Diuretic in Mechanically Ventilated Children with Viral Bronchiolitis: a Cohort Study.

J Crit Care Med (Targu Mures) 2021 Apr 12;7(2):97-103. Epub 2021 May 12.

Department of Pediatrics, Division of Critical Care Medicine, Nemours A.I. duPont Hospital for Children, Wilmington, DE, USA.

Introduction: Viral bronchiolitis is a leading cause of admissions to pediatric intensive care unit (PICU). A literature review indicates that there is limited information on fluid overload and the use of diuretics in mechanically ventilated children with viral bronchiolitis. This study was conducted to understand diuretic use concerning fluid overload in this population. Read More

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SMAD7 and SMAD4 expression in colorectal cancer progression and therapy response.

Exp Mol Pathol 2021 Oct 28;123:104714. Epub 2021 Oct 28.

Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, 11 000 Belgrade, Serbia.

Inhibitory SMAD7 and common mediator SMAD4 play crucial roles in SMAD-dependent TGF-β signaling that is often disrupted in colorectal cancer (CRC). This study aimed to profile the expression of SMAD7 and SMAD4 in primary and metastatic CRC and to evaluate their significance in disease progression and therapy response. The expression of SMAD7 and SMAD4 genes was analyzed by quantitative real-time PCR in tissues from 35 primary and metastatic CRC patients and in vitro in 7 human cell lines originating from colon tissue. Read More

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October 2021

Incidence of Brucella infection in various livestock species raised under the pastoral production system in Isiolo County, Kenya.

BMC Vet Res 2021 Oct 30;17(1):342. Epub 2021 Oct 30.

International Livestock Research Institute, Nairobi, Kenya.

Background: We implemented a longitudinal study to determine the incidence of Brucella infection in cattle, camels, sheep and goats that were being raised in a pastoral area in Isiolo County, Kenya. An initial cross-sectional survey was implemented to identify unexposed animals for follow up; that survey used 141 camels, 216 cattle, 208 sheep and 161 goats. Sera from these animals were screened for Brucella spp. Read More

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October 2021

Quantification of airborne SARS-CoV-2 genomic particles in different hospital settings.

Sci Rep 2021 10 28;11(1):21284. Epub 2021 Oct 28.

LIM-05, Department of Pathology, Faculty of Medicine, University of São Paulo, São Paulo, Brazil.

We quantified the presence of SARS-CoV-2 RNA in the air of different hospital settings and the autopsy room of the largest medical centre in Sao Paulo, Brazil. Real-time reverse-transcription PCR was used to determine the presence of the envelope protein of SARS-CoV-2 and the nucleocapsid protein genes. The E-gene was detected in 5 out of 6 samples at the ICU-COVID-19 ward and in 5 out of 7 samples at the ward-COVID-19. Read More

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October 2021

First detection and genetic identification of Rickettsia infection in Rhipicephalus sanguineus (Acari: Ixodidae) ticks collected from Southern Taiwan.

Exp Appl Acarol 2021 Dec 28;85(2-4):291-304. Epub 2021 Oct 28.

M.Sc. Program in Tropical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, 807, Taiwan, Republic of China.

The prevalence and genetic identity of Rickettsia infection in Rhipicephalus sanguineus sensu lato (s.l.) ticks were determined in Taiwan. Read More

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December 2021

Hypocaloric Dieting Unsettles the Neuroenergetic Homeostasis in Humans.

Nutrients 2021 Sep 28;13(10). Epub 2021 Sep 28.

Section of Psychoneurobiology, Center of Brain, Behavior and Metabolism, University of Luebeck, Ratzeburger Allee 160, 23538 Luebeck, Germany.

Background: The effects of low-calorie dieting in obesity are disappointing in the long run. The brain's energy homeostasis plays a key role in the regulation of body weight. We hypothesized that the cerebral energy status underlies an adaptation process upon body weight loss due to hypocaloric dieting in humans. Read More

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September 2021

Overexpression of Long Non-Coding RNA Linc01315 Predicts Poor Prognosis in Breast Cancer.

Front Oncol 2021 5;11:562378. Epub 2021 Oct 5.

Key Laboratory of Breast Cancer in Shanghai, Department of Breast Surgery, Fudan University Shanghai Cancer Center, Shanghai, China.

Background: LncRNAs have been shown to play critical roles in regulating tumorigenesis and tumor progression. Using LncRNAs to predict prognosis and therapeutic response to cancer treatment has been caused for concern, but the predictive value of lncRNAs remains to be explored and underlying mechanisms have not been completely understood.

Methods: The Linc01315 expression level was detected in 282 breast cancer tissues by using quantitative RT-PCR. Read More

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October 2021

Association of four genetic variants with colorectal cancer in Kazakhstan population.

Oncotarget 2021 Oct 12;12(21):2215-2222. Epub 2021 Oct 12.

Department of Strategic Development and Science, Karaganda Medical University, Karaganda 100008, Kazakhstan.

The study was conducted to search for polymorphisms located in the 10th chromosome associated with colorectal adenocarcinoma in representatives of the Kazakhstan population. Study was performed with 282 colorectal cancer (CRC) patients and 159 controls. Genotyping of SNPs was performed by QuantStudio 12K Flex PCR. Read More

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October 2021