3,651 results match your criteria -atpase modulation

Curcumin Attenuates Sarcopenia in Chronic Forced Exercise Executed Aged Mice by Regulating Muscle Degradation and Protein Synthesis with Antioxidant and Anti-inflammatory Effects.

J Agric Food Chem 2021 May 5. Epub 2021 May 5.

Department of Food Science and Biotechnology, Sejong University, 209, Neungdong-ro, Gwangjin-gu, Seoul 05006, Republic of Korea.

The aim of the current study is to investigate the effects of spray dry powders of containing 40% curcumin (CM-SD), as a new aqueous curcumin formula, on sarcopenia in chronic forced exercise executed 10 month old ICR mice. CM-SD (80 and 40 mg/kg) increased calf thicknesses and strengths, total body and calf protein amounts, and muscle weights in both gastrocnemius and soleus muscles. mRNA expressions regarding muscle growth and protein synthesis were induced, while those of muscle degradation significantly declined in CM-SD treatment. Read More

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Allosteric modulation of cardiac myosin mechanics and kinetics by the conjugated omega-7,9 trans-fat rumenic acid.

J Physiol 2021 May 4. Epub 2021 May 4.

Institute for Biophysical Chemistry, OE4350, Medizinische Hochschule Hannover, Hannover, 30625, Germany.

Key Points: Direct binding of rumenic acid to the cardiac myosin-2 motor domain increases the release rate for orthophosphate and increases the Ca responsiveness of cardiac muscle at low load. Physiological cellular concentrations of rumenic acid affect the ATP turnover rates of the super-relaxed and disordered relaxed states of β-cardiac myosin leading to a net increase in myocardial metabolic load. In Ca -activated trabeculae, rumenic acid exerts a direct inhibitory effect on the force-generating mechanism without affecting the number of force-generating motors. Read More

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ATP2C2 Has Potential to Define Tumor Microenvironment in Breast Cancer.

Front Immunol 2021 14;12:657950. Epub 2021 Apr 14.

Key Laboratory of Molecular Oncology and Epigenetics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Tumor microenvironment (TME) is vital for the occurrence and development of breast cancer (BRCA). However, it remains challenging to understand the dynamic modulation of the stromal and immune components comprehensively in TME. Herein, we used ESTIMATE and CIBERSORT algorithm to estimate the number of stromal and immune components and the abundance of tumor-infiltrating immune cells (TICs) in 582 BRCA cases from gene expression omnibus (GEO) database. Read More

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Na/K-ATPase as a Target of Cardiac Glycosides for the Treatment of SARS-CoV-2 Infection.

Front Pharmacol 2021 15;12:624704. Epub 2021 Apr 15.

Laboratório de Imunofarmacologia, Departamento de Bioquímica, Universidade Federal Do Estado Do Rio de Janeiro, Rio de Janeiro, Brazil.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), identified for the first time in Wuhan, China, causes coronavirus disease 2019 (COVID-19), which moved from epidemic status to becoming a pandemic. Since its discovery in December 2019, there have been countless cases of mortality and morbidity due to this virus. Several compounds such as chloroquine, hydroxychloroquine, lopinavir-ritonavir, and remdesivir have been tested as potential therapies; however, no effective treatment is currently recommended by regulatory agencies. Read More

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Computer-aided search for 5-arylideneimidazolone anticancer agents able to overcome ABCB1-based multidrug resistance.

ChemMedChem 2021 Apr 30. Epub 2021 Apr 30.

Uniwersytet Jagiellonski w Krakowie Collegium Medicum, Dept. of Technology and Biotechnology of Drugs, ul. Medyczna 9, Not Available, 30-688, Cracow, POLAND.

ABCB1 modulation is an interesting strategy in search for new anticancer agents overcoming multidrug resistance (MDR). Hence,  17 new 5-arylideneimidazolones containing amine moiety, as potential ABCB1 inhibitors was designed, synthesized, and investigated. The series was tested in both, parental (PAR) and multidrug resistant (MDR) overproducing ABCB1, T-lymphoma cancer cells using cytotoxicity assay. Read More

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Dietary Bioactive Diindolylmethane Enhances the Therapeutic Efficacy of Centchroman in Breast Cancer Cells by Regulating ABCB1/P-gp Efflux Transporter.

J Nutr Biochem 2021 Apr 25:108749. Epub 2021 Apr 25.

Department of Biochemistry, CSIR-Central Food Technological Research Institute, Mysore, India-570020; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad- 201002, India. Electronic address:

Overexpression of drug efflux transporters is commonly associated with multidrug-resistance in cancer therapy. Here for the first time, we investigated the ability of diindolylmethane (DIM), a dietary bioactive rich in cruciferous vegetables, in enhancing the efficacy of Centchroman (CC) by modulating the drug efflux transporters in human breast cancer cells. CC is a selective estrogen receptor modulator, having promising therapeutic efficacy against breast cancer. Read More

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Impact of physical activity on mitochondrial enzymes, muscle stem cell and anti-oxidant protein Sestrins in Sarcopenic mice.

Exp Gerontol 2021 Apr 16;150:111358. Epub 2021 Apr 16.

Department of Geriatric Medicine, All India Institute of Medical Sciences, New Delhi, India. Electronic address:

Introduction: Sarcopenia is the loss of skeletal muscle mass and function. It is a major health issue in old age due to lack of understanding of the origin and molecular mechanism. Altered dietary pattern, sedentary lifestyle and physical inactivity have shown adverse effect of skeletal muscle function. Read More

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EpCAM promotes endosomal modulation of the cortical RhoA zone for epithelial organization.

Nat Commun 2021 04 13;12(1):2226. Epub 2021 Apr 13.

Cell Adhesion and Mechanics, Institut Jacques Monod, CNRS UMR7592, Paris Diderot University, Paris, France.

At the basis of cell shape and behavior, the organization of actomyosin and its ability to generate forces are widely studied. However, the precise regulation of this contractile network in space and time is unclear. Here, we study the role of the epithelial-specific protein EpCAM, a contractility modulator, in cell shape and motility. Read More

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Effect of Dynamic and Static Load on the Concentration of Myokines in the Blood Plasma and Content of Sodium and Potassium in Mouse Skeletal Muscles.

Biochemistry (Mosc) 2021 Mar;86(3):370-381

National Research Tomsk State University, Tomsk, 634050, Russia.

Modulation of cytokine production by physical activity is of considerable interest, since it might be a promising strategy for correcting metabolic processes at both cellular and systemic levels. The content of IL-6, IL-8, and IL-15 in the plasma and the concentration of monovalent cations in the skeletal muscles of trained and untrained mice were studied at different periods after static and dynamic exercises. Dynamic loads caused an increase in the IL-6 content and decrease in the IL-15 content in the plasma of untrained mice, but produced no effect on the concentration of IL-8. Read More

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Extracellular vesicles regulate purinergic signaling and epithelial sodium channel expression in renal collecting duct cells.

FASEB J 2021 May;35(5):e21506

Department of Physiology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, the Netherlands.

Purinergic signaling regulates several renal physiological and pathophysiological processes. Extracellular vesicles (EVs) are nanoparticles released by most cell types, which, in non-renal tissues, modulate purinergic signaling. The aim of this study was to investigate the effect of EVs from renal proximal tubule (HK2) and collecting duct cells (HCD) on intra- and intersegment modulation of extracellular ATP levels, the underlying molecular mechanisms, and the impact on the expression of the alpha subunit of the epithelial sodium channel (αENaC). Read More

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Pleiotropic, non-cell death-associated effects of inhibitors of receptor-interacting protein kinase 1 in the heart.

Mol Cell Biochem 2021 Apr 3. Epub 2021 Apr 3.

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Comenius University in Bratislava, Odbojarov 10, 832 32, Bratislava, Slovak Republic.

Inhibition of receptor-interacting protein kinase 1 (RIP1) has been recognized as a compelling tool for limiting necroptosis. Recent findings have indicated that RIP1 inhibitor, necrostatin-1 (Nec-1), is also able to modify heart function under non-cell death conditions. In this study, we investigated its underlying molecular mechanisms and compared with those of novel pharmacologically improved agents (Nec-1s and GSK'772) and its inactive analog (Nec-1i). Read More

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Dynamic Remodeling of Membranes and Their Lipids during Acute Hormone-Induced Steroidogenesis in MA-10 Mouse Leydig Tumor Cells.

Int J Mol Sci 2021 Mar 4;22(5). Epub 2021 Mar 4.

The Research Institute, McGill University Health Centre, Montreal, QC H4A 3J1, Canada.

Lipids play essential roles in numerous cellular processes, including membrane remodeling, signal transduction, the modulation of hormone activity, and steroidogenesis. We chose steroidogenic MA-10 mouse tumor Leydig cells to investigate subcellular lipid localization during steroidogenesis. Electron microscopy showed that cAMP stimulation increased associations between the plasma membrane (PM) and the endoplasmic reticulum (ER) and between the ER and mitochondria. Read More

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Na/K-ATPase Revisited: On Its Mechanism of Action, Role in Cancer, and Activity Modulation.

Molecules 2021 Mar 28;26(7). Epub 2021 Mar 28.

Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 3, 166 28 Prague 6, Czech Republic.

Maintenance of Na and K gradients across the cell plasma membrane is an essential process for mammalian cell survival. An enzyme responsible for this process, sodium-potassium ATPase (NKA), has been currently extensively studied as a potential anticancer target, especially in lung cancer and glioblastoma. To date, many NKA inhibitors, mainly of natural origin from the family of cardiac steroids (CSs), have been reported and extensively studied. Read More

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Fronto-temporal dementia risk gene has opposing effects in different lysosomal storage disorders.

Brain Commun 2021 16;3(1):fcaa200. Epub 2020 Nov 16.

Cellular Neuroscience, Neurodegeneration and Repair Program, Yale University School of Medicine, New Haven, CT, USA.

TMEM106B is a transmembrane protein localized to the endo-lysosomal compartment. Genome-wide association studies have identified as a risk modifier of Alzheimer's disease and frontotemporal lobar degeneration, especially with progranulin haploinsufficiency. We recently demonstrated that TMEM106B loss rescues progranulin null mouse phenotypes including lysosomal enzyme dysregulation, neurodegeneration and behavioural alterations. Read More

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November 2020

The Na/K-ATPase α1/Src interaction regulates metabolic reserve and Western diet intolerance.

Acta Physiol (Oxf) 2021 Mar 22:e13652. Epub 2021 Mar 22.

Marshall Institute for Interdisciplinary Research, Marshall University, Huntington, WV, USA.

Aim: Highly prevalent diseases such as insulin resistance and heart failure are characterized by reduced metabolic flexibility and reserve. We tested whether Na/K-ATPase (NKA)-mediated regulation of Src kinase, which requires two NKA sequences specific to the α1 isoform, is a regulator of metabolic capacity that can be targeted pharmacologically.

Methods: Metabolic capacity was challenged functionally by Seahorse metabolic flux analyses and glucose deprivation in LLC-PK1-derived cells expressing Src binding rat NKA α1, non-Src-binding rat NKA α2 (the most abundant NKA isoform in the skeletal muscle), and Src binding gain-of-function mutant rat NKA α2. Read More

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Lysophosphatidic acid (LPA) as a modulator of plasma membrane Ca-ATPase from basolateral membranes of kidney proximal tubules.

J Physiol Biochem 2021 Mar 11. Epub 2021 Mar 11.

Laboratório de Biomembranas, Instituto de Biofísica Carlos Chagas Filho, CCS, Universidade Federal do Rio de Janeiro, 21949-902, Ilha do Fundão, Rio de Janeiro, Brasil.

Lysophosphatidic acid (LPA) acts through the activation of G protein-coupled receptors, in a Ca-dependent manner. We show the effects of LPA on the plasma membrane Ca-ATPase (PMCA) from kidney proximal tubule cells. The Ca-ATPase activity was inhibited by nanomolar concentrations of LPA, with maximal inhibition (~50%) obtained with 20 nM LPA. Read More

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Sarco/Endoplasmic Reticulum Ca Transporting ATPase (SERCA) Modulates Autophagic, Inflammatory, and Mitochondrial Responses during Influenza A Virus Infection in Human Lung Cells.

J Virol 2021 Mar 10. Epub 2021 Mar 10.

State Key Lab of Respiratory Disease, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou, China

Influenza A virus is an important human pathogen causing significant morbidity and mortality. Numerous host factors and cellular responses are dysregulated during influenza A virus infection. This includes arrest of autophagic flux dependent on the influenza M2 ion channel, but little is known which host factors participate in this autophagic dysfunction. Read More

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A differentiated Ca signalling phenotype has minimal impact on myocardin expression in an automated differentiation assay using A7r5 cells.

Cell Calcium 2021 Feb 17;96:102369. Epub 2021 Feb 17.

Biomedical Physiology & Kinesiology, Simon Fraser University, Burnaby, Canada; Centre for Cell Biology, Development, and Disease, Simon Fraser University, Burnaby, BC, Canada. Electronic address:

Vascular smooth muscle cells are unusual in that differentiated, contractile cells possess the capacity to "de-differentiate" into a synthetic phenotype that is characterized by being replicative, secretory, and migratory. One aspect of this phenotypic modulation is a shift from voltage-gated Ca signalling in electrically coupled, differentiated cells to increased dependence on store-operated Ca entry and sarcoplasmic reticulum Ca release in synthetic cells. Conversely, an increased voltage-gated Ca entry is seen when proliferating A7r5 smooth muscle cells quiesce. Read More

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February 2021

Copper Dependent Modulation of α-Synuclein Phosphorylation in Differentiated SHSY5Y Neuroblastoma Cells.

Int J Mol Sci 2021 Feb 18;22(4). Epub 2021 Feb 18.

Department of Biological and Environmental Science and Technology, University of Salento, 73100 Lecce, Italy.

Copper (Cu) dyshomeostasis plays a pivotal role in several neuropathologies, such as Parkinson's disease (PD). Metal accumulation in the central nervous system (CNS) could result in loss-of-function of proteins involved in Cu metabolism and redox cycling, generating reactive oxygen species (ROS). Moreover, neurodegenerative disorders imply the presence of an excess of misfolded proteins known to lead to neuronal damage. Read More

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February 2021

Effect of a purine derivative containing selenium to improve memory decline and anxiety through modulation of the cholinergic system and Na/K-ATPase in an Alzheimer's disease model.

Metab Brain Dis 2021 Jun 2;36(5):871-888. Epub 2021 Mar 2.

Programa de Pós-graduação em Bioquímica e Bioprospecção, Laboratório de Pesquisa em Farmacologia Bioquímica (LaFarBio), Grupo de Pesquisa em Neurobiotecnologia (GPN), Centro de Ciências Químicas, Farmacêuticas e de Alimentos (CCQFA), Universidade Federal de Pelotas (UFPel), Campus Capão do Leão, Pelotas, RS, CEP96010-900, Brazil.

Alzheimer's disease (AD) is a worldwide problem, and there are currently no treatments that can stop this disease. To investigate the binding affinity of 6-((4-fluorophenyl) selanyl)-9H-purine (FSP) with acetylcholinesterase (AChE), to verify the effects of FSP in an AD model in mice and to evaluate the toxicological potential of this compound in mice. The binding affinity of FSP with AChE was investigated by molecular docking analyses. Read More

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The Janus face of ouabain in Na /K -ATPase and calcium signalling in neurons.

Br J Pharmacol 2021 Feb 28. Epub 2021 Feb 28.

Laboratory of Molecular Neuropharmacology, Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.

Na /K -ATPase, a transmembrane protein essential for maintaining the electrochemical gradient across the plasma membrane, acts as a receptor for cardiotonic steroids such as ouabain. Cardiotonic steroids binding to Na /K -ATPase triggers signalling pathways or inhibits Na /K -ATPas activity in a concentration-dependent manner, resulting in a modulation of Ca levels, which are essential for homeostasis in neurons. However, most of the pharmacological strategies for avoiding neuronal death do not target Na /K -ATPase activity due to its complexity and the poor understanding of the mechanisms involved in Na /K -ATPase modulation. Read More

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February 2021

Transcriptome Profiling of Long Non-coding RNAs During the Atlantic Salmon Smoltification Process.

Mar Biotechnol (NY) 2021 Apr 27;23(2):308-320. Epub 2021 Feb 27.

Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, Concepción, Chile.

For salmon aquaculture, one of the most critical phase is the parr-smolt transformation. Studies around this process have mainly focused on physiological changes and the Na/K-ATPase activity during the osmoregulatory activity. However, understanding how the salmon genome regulates the parr-smolt transformation, specifically the molecular mechanisms involved, remains uncovered. Read More

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Role of the V1G1 subunit of V-ATPase in breast cancer cell migration.

Sci Rep 2021 Feb 25;11(1):4615. Epub 2021 Feb 25.

Department of Biological and Environmental Sciences and Technologies, University of Salento, Via Provinciale Lecce-Monteroni n. 165, 73100, Lecce, Italy.

V-ATPase is a large multi-subunit complex that regulates acidity of intracellular compartments and of extracellular environment. V-ATPase consists of several subunits that drive specific regulatory mechanisms. The V1G1 subunit, a component of the peripheral stalk of the pump, controls localization and activation of the pump on late endosomes and lysosomes by interacting with RILP and RAB7. Read More

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February 2021

C-type natriuretic peptide-induced relaxation through cGMP-dependent protein kinase and SERCA activation is impaired in two kidney-one clip rat aorta.

Life Sci 2021 May 18;272:119223. Epub 2021 Feb 18.

Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Department of Physics and Chemistry, Ribeirão Preto, SP, Brazil.

Aims: Hypertension underlies endothelial dysfunction, and activation of vasorelaxation signaling with low dependence on nitric oxide (NO) represents a good alternative for vascular modulation. C-type natriuretic peptide (CNP) causes relaxation by increasing cyclic guanosine 3',5'-monophosphate (cGMP) or Gi-protein activation through its natriuretic peptide receptor-B or -C, respectively. We have hypothesized that CNP could exerts its effects and could overcome endothelial dysfunction in two kidney-one clip (2K-1C) hypertensive rat aorta. Read More

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Inactivation of SERCA2 Cys accelerates aortic aneurysms by suppressing PPARγ.

Br J Pharmacol 2021 Feb 16. Epub 2021 Feb 16.

Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, School of Pharmaceutical Sciences, Chongqing University, Chongqing, China.

Background And Purpose: Inactivation of Cys (C674) in the sarcoplasmic/endoplasmic reticulum Ca ATPase 2 (SERCA2) causes intracellular Ca accumulation, which activates calcineurin-mediated nuclear factor of activated T-lymphocytes (NFAT)/NF-κB pathways, and results in the phenotypic modulation of smooth muscle cells (SMCs) to accelerate angiotensin II-induced aortic aneurysms. Our goal was to investigate the mechanism involved.

Experimental Approach: We used heterozygous SERCA2 C674S knock-in (SKI) mice, where half of C674 was substituted by serine, to mimic partial irreversible oxidation of C674. Read More

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February 2021

Neuroprotective effect of Betalain against AlCl-induced Alzheimer's disease in Sprague Dawley Rats via putative modulation of oxidative stress and nuclear factor kappa B (NF-κB) signaling pathway.

Biomed Pharmacother 2021 May 12;137:111369. Epub 2021 Feb 12.

Basic Theory of Traditional Chinese Medicine, School of Basic Medicine, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, China. Electronic address:

Alzheimer's disease (AD) is the most progressive form of neurodegenerative disease, which severely impairs cognitive function. Oxidative stress is identified to contribute to the mechanisms responsible for the pathogenesis of such neurodegenerative diseases. Aluminum is a potent neurotoxin for inducing oxidative stress associated with neurodegenerative diseases. Read More

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Modulation of Ion Transport Across Plant Membranes by Polyamines: Understanding Specific Modes of Action Under Stress.

Front Plant Sci 2020 26;11:616077. Epub 2021 Jan 26.

International Research Centre for Environmental Membrane Biology, Foshan University, Foshan, China.

This work critically discusses the direct and indirect effects of natural polyamines and their catabolites such as reactive oxygen species and γ-aminobutyric acid on the activity of key plant ion-transporting proteins such as plasma membrane H and Ca ATPases and K-selective and cation channels in the plasma membrane and tonoplast, in the context of their involvement in stress responses. Docking analysis predicts a distinct binding for putrescine and longer polyamines within the pore of the vacuolar TPC1/SV channel, one of the key determinants of the cell ionic homeostasis and signaling under stress conditions, and an additional site for spermine, which overlaps with the cytosolic regulatory Ca-binding site. Several unresolved problems are summarized, including the correct estimates of the subcellular levels of polyamines and their catabolites, their unexplored effects on nucleotide-gated and glutamate receptor channels of cell membranes and Ca-permeable and K-selective channels in the membranes of plant mitochondria and chloroplasts, and pleiotropic mechanisms of polyamines' action on H and Ca pumps. Read More

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January 2021

In Vitro Modulation of P-Glycoprotein Activity by Essential Oil on Acute Myeloid Leukemia Cell Line HL-60R.

Pharmaceuticals (Basel) 2021 Jan 31;14(2). Epub 2021 Jan 31.

Department of Biological, Chemical and Pharmaceutical Science and Technology (STEBICEF), University of Palermo, 90128 Palermo, Italy.

species have a large spectrum of traditional medicinal uses. We tested the biological activities of the essential oil (EO) of Drake in an acquired multidrug resistance leukemia model to assess whether the EO obtained by hydrodistillation of stems was able to reverse the resistant phenotype. HL-60R cell lines are characterized by the overexpression of P-glycoprotein (P-gp), inhibitors of apoptosis proteins (IAPs) and constitutive expression of NF-κB. Read More

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January 2021

Mitochondria-cytokine crosstalk following skeletal muscle injury and disuse: a mini-review.

Am J Physiol Cell Physiol 2021 05 10;320(5):C681-C688. Epub 2021 Feb 10.

Department of Kinesiology, University of Georgia, Athens, Georgia.

Skeletal muscle mitochondria are highly adaptable, highly dynamic organelles that maintain the functional integrity of the muscle fiber by providing ATP for contraction and cellular homeostasis (e.g., Na/K ATPase). Read More

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Structural elements in the flexible tail of the co-chaperone p23 coordinate client binding and progression of the Hsp90 chaperone cycle.

Nat Commun 2021 02 5;12(1):828. Epub 2021 Feb 5.

Department of Chemistry, Technische Universität München, Garching, Germany.

The co-chaperone p23 is a central part of the Hsp90 machinery. It stabilizes the closed conformation of Hsp90, inhibits its ATPase and is important for client maturation. Yet, how this is achieved has remained enigmatic. Read More

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February 2021