62,062 results match your criteria -atpase activity

Atp23p and Atp10p coordinate to regulate the assembly of yeast mitochondrial ATP synthase.

FASEB J 2021 Jun;35(6):e21538

Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, Center for Human Genome Research, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.

Two chaperones, Atp23p and Atp10p, were previously shown to regulate the assembly of yeast mitochondrial ATP synthase, and extra expression of ATP23 was found to partially rescue an atp10 deletion mutant, by an unknown mechanism. Here, we identified that the residues 112-115 (LRDK) of Atp23p were required for its function in assisting assembly of the synthase, and demonstrated both functions of Atp23p, processing subunit 6 precursor and assisting assembly of the synthase, were required for the partial rescue of atp10 deletion mutant. By chasing labeling with isotope S-methionine, we found the stability of subunit 6 of the synthase increased in atp10 null strain upon overexpression of ATP23. Read More

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A Novel Mechanism for Activation of Myosin Regulatory Light Chain by Protein Kinase C-Delta in Drosophila.

Genetics 2020 Sep;216(1):177-190

Department of Biology, San Diego State University, San Diego, California 92182.

Myosin is an essential motor protein, which in muscle is comprised of two molecules each of myosin heavy-chain (MHC), the essential or alkali myosin light-chain 1 (MLC1), and the regulatory myosin light-chain 2 (MLC2). It has been shown previously that MLC2 phosphorylation at two canonical serine residues is essential for proper flight muscle function in Drosophila; however, MLC2 is also phosphorylated at additional residues for which the mechanism and functional significance is not known. We found that a hypomorphic allele of Pkcδ causes a flightless phenotype; therefore, we hypothesized that PKCδ phosphorylates MLC2. Read More

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September 2020

Cryo-EM structure of ABCG5/G8 in complex with modulating antibodies.

Commun Biol 2021 May 5;4(1):526. Epub 2021 May 5.

Department of Therapeutics Discovery, Amgen Research, Amgen Inc., South San Francisco, CA, USA.

The heterodimer of ATP-binding cassette transporter ABCG5 and ABCG8 mediates the excretion of sterols from liver and intestine, playing a critical role in cholesterol homeostasis. Here, we present the cryo-EM structure of ABCG5/G8 in complex with the Fab fragments from two monoclonal antibodies at 3.3Å resolution. Read More

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[Amyloid β hypothesis in Alzheimer's disease and Cl-ATPase-Neuronal cell death via PI4KIIα inhibition and recovery agents].

Chiyoko Inagaki

Nihon Yakurigaku Zasshi 2021 ;156(3):166-170

Kansai Medical University.

In the brains of patients with Alzheimer's disease, a decrease in phosphatidylinositol phosphate (PIP) requiring Cl-ATPase activity was found. In cultured rat hippocampal neurons, pathophysiological concentrations of amyloid β proteins (Aβs≤10 nM) lowered PIP levels and Cl-ATPase activity with an increase in intracellular Cl concentrations, resulting in Cl-dependent enhancements in glutamate neurotoxicity and, ultimately, neuronal cell death. Pathophysiological concentrations of Aβs(0. Read More

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January 2021

Construction of a SUMOylation regulator-based prognostic model in low-grade glioma.

J Cell Mol Med 2021 May 5. Epub 2021 May 5.

Department of Stomatology, Shengjing Hospital of China Medical University, Shenyang, China.

Low-grade glioma (LGG) is an intracranial malignant tumour that mainly originates from astrocytes and oligodendrocytes. SUMOylation is one of the post-translational modifications but studies of SUMOylation in LGG is quite limited. Transcriptome data, single nucleotide variant (SNV) data and clinical data of LGG were derived from public databases. Read More

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Intermediates in allosteric equilibria of DnaK-ATP interactions with substrate peptides.

Acta Crystallogr D Struct Biol 2021 May 14;77(Pt 5):606-617. Epub 2021 Apr 14.

Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.

Hsp70 molecular chaperones facilitate protein disaggregation and proper folding through iterative cycles of polypeptide binding and release that are allosterically coupled to ATP binding and hydrolysis. Hsp70s are ubiquitous and highly conserved across all of life; they bind ATP at an N-terminal nucleotide-binding domain (NBD) and client peptides in the substrate-binding domain (SBD). The NBD and SBD are connected by a highly conserved linker segment that is integrated into the NBD when ATP is bound but is flexible when the NBD is nucleotide-free or bound with ADP. Read More

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Cardiac Glycosides as Immune System Modulators.

Biomolecules 2021 Apr 29;11(5). Epub 2021 Apr 29.

Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 3, 166 28 Prague 6, Czech Republic.

Cardiac glycosides (CGs) are natural steroid compounds occurring both in plants and animals. They are known for long as cardiotonic agents commonly used for various cardiac diseases due to inhibition of Na/K-ATPase (NKA) pumping activity and modulating heart muscle contractility. However, recent studies show that the portfolio of diseases potentially treatable with CGs is much broader. Read More

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Antifungal Activity of Novel Formulations Based on Terpenoid Prodrugs against in a Mouse Model.

Pharmaceutics 2021 Apr 29;13(5). Epub 2021 Apr 29.

Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, St. John's University, 8000 Utopia Parkway, Jamaica, NY 11439, USA.

Carvacrol (CAR), a phenolic monoterpenoid, has been extensively investigated for its antimicrobial and antifungal activity. As a result of its poor physicochemical properties, water soluble carvacrol prodrugs (WSCPs) with improved water solubility were previously synthesized and found to possess antimicrobial activity. Here, three novel CAR analogs, WSCP1, WSCP2, and WSCP3, were tested against fluconazole (FLU)-sensitive and -resistant strains where they showed greater antifungal activity than CAR against . Read More

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Quality of frozen porcine Longissimus lumborum muscles injected with l-arginine and l-lysine solution.

Meat Sci 2021 Apr 28;179:108530. Epub 2021 Apr 28.

Engineering Research Center of Bio-Process, Ministry of Education, Hefei, University of Technology, Hefei 230009, Anhui, PR China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China. Electronic address:

This study investigated the effects of l-arginine and l-lysine on the water holding capacity, shear force, color, and protein denaturation of frozen porcine Longissimus lumborum. Four batches were prepared, each corresponding to samples of an experimental treatment: without a cryoprotective solution, injecting a 0.3% sodium tripolyphosphate and 0. Read More

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Aloperine improves osteoporosis in ovariectomized mice by inhibiting RANKL-induced NF-κB, ERK and JNK approaches.

Int Immunopharmacol 2021 May 1;97:107720. Epub 2021 May 1.

Department of Spinal Surgery, the First Affiliated Hospital, University of South China, Hengyang, Hunan 421000, China. Electronic address:

Presently, postmenopausal osteoporosis mainly caused by excessive activation of in vivo osteoclasts has become a global public health burden. Natural compounds have gradually become the potential drugs for the treatment of postmenopausal osteoporosis. Aloperine is a new alkaloid extracted from the leaves and seeds of sophora bean. Read More

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Allosteric modulation of cardiac myosin mechanics and kinetics by the conjugated omega-7,9 trans-fat rumenic acid.

J Physiol 2021 May 4. Epub 2021 May 4.

Institute for Biophysical Chemistry, OE4350, Medizinische Hochschule Hannover, Hannover, 30625, Germany.

Key Points: Direct binding of rumenic acid to the cardiac myosin-2 motor domain increases the release rate for orthophosphate and increases the Ca responsiveness of cardiac muscle at low load. Physiological cellular concentrations of rumenic acid affect the ATP turnover rates of the super-relaxed and disordered relaxed states of β-cardiac myosin leading to a net increase in myocardial metabolic load. In Ca -activated trabeculae, rumenic acid exerts a direct inhibitory effect on the force-generating mechanism without affecting the number of force-generating motors. Read More

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Kinetic and structural parameters governing Fic-mediated adenylylation/AMPylation of the Hsp70 chaperone, BiP/GRP78.

Cell Stress Chaperones 2021 May 3. Epub 2021 May 3.

From the Department of Biological Sciences, Purdue University, 915 W. State St., LILY G-227, West Lafayette, IN, 47907, USA.

Fic (filamentation induced by cAMP) proteins regulate diverse cell signaling events by post-translationally modifying their protein targets, predominantly by the addition of an AMP (adenosine monophosphate). This modification is called Fic-mediated adenylylation or AMPylation. We previously reported that the human Fic protein, HYPE/FicD, is a novel regulator of the unfolded protein response (UPR) that maintains homeostasis in the endoplasmic reticulum (ER) in response to stress from misfolded proteins. Read More

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TRPC7 regulates the electrophysiological functions of embryonic stem cell-derived cardiomyocytes.

Stem Cell Res Ther 2021 May 3;12(1):262. Epub 2021 May 3.

School of Life Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China.

Background: Biological pacemakers consisting of pluripotent stem cell-derived cardiomyocytes are potentially useful for treating bradycardia. However, tachyarrhythmia caused by derived cardiomyocytes themselves is one of main barriers hampering their clinical translation. An in-depth understanding of the mechanisms underlying the spontaneous action potential (a. Read More

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ATPase and helicase activities of porcine epidemic diarrhea virus nsp13.

Vet Microbiol 2021 Apr 26;257:109074. Epub 2021 Apr 26.

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China; The Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China. Electronic address:

Porcine epidemic diarrhea virus (PEDV) is a reemerging Alphacoronavirus that causes lethal diarrhea in piglets. Coronavirus nonstructural protein 13 (nsp13) encodes helicase, which plays pivotal roles during viral replication by unwinding viral RNA. However, the biochemical characterization of PEDV nsp13 remains largely unknown. Read More

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Heat Shock Protein 90 Inhibitor Effects on Pancreatic Cancer Cell Cultures.

Pancreas 2021 Apr;50(4):625-632

Departments of Pathology and Oncology, Johns Hopkins University School of Medicine, Baltimore, MD.

Objectives: Pancreatic ductal adenocarcinoma is one of the deadliest cancers for which few curative therapies are available to date. Heat shock protein 90 (Hsp90) inhibitors have shown activity against numerous cancers in vitro; therefore, we tested whether they could be used to target pancreatic ductal adenocarcinoma.

Methods: Inhibitors of Hsp90 ATPase activity were applied on low-passage pancreatic cell line cultures (Panc10. Read More

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Na/K-ATPase as a Target of Cardiac Glycosides for the Treatment of SARS-CoV-2 Infection.

Front Pharmacol 2021 15;12:624704. Epub 2021 Apr 15.

Laboratório de Imunofarmacologia, Departamento de Bioquímica, Universidade Federal Do Estado Do Rio de Janeiro, Rio de Janeiro, Brazil.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), identified for the first time in Wuhan, China, causes coronavirus disease 2019 (COVID-19), which moved from epidemic status to becoming a pandemic. Since its discovery in December 2019, there have been countless cases of mortality and morbidity due to this virus. Several compounds such as chloroquine, hydroxychloroquine, lopinavir-ritonavir, and remdesivir have been tested as potential therapies; however, no effective treatment is currently recommended by regulatory agencies. Read More

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Glaucocalyxin A suppresses osteoclastogenesis induced by RANKL and osteoporosis induced by ovariectomy by inhibiting the NF-κB and Akt pathways.

J Ethnopharmacol 2021 Apr 29:114176. Epub 2021 Apr 29.

Department of Orthopedics, The First Affiliated Hospital of Nanchang University, Artificial Joints Engineering and Technology Research Center of Jiangxi Province, Nanchang, Jiangxi province, 330006, China. Electronic address:

Ethnopharmacological Relevance: Glaucocalyxin A (GLA), the most abundant active component of the aboveground sections of Rabdosia japonica (Burm. f.) Hara var. Read More

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Novel insights into ATP-stimulated cleavage of branched DNA and RNA substrates through structure-guided studies of the Holliday junction resolvase RuvX.

J Mol Biol 2021 Apr 29:167014. Epub 2021 Apr 29.

Department of Biochemistry. Electronic address:

Much of our understanding of the homologous recombination (HR) machinery hinges on studies using Escherichia coli as a model organism. Interestingly enough, studies on the HR machinery in different bacterial species casts doubt on the universality of the E. coli paradigm. Read More

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Quantitative model analysis of the resting membrane potential in insect skeletal muscle: Implications for low temperature tolerance.

Comp Biochem Physiol A Mol Integr Physiol 2021 Apr 28;257:110970. Epub 2021 Apr 28.

Department of Biomedicine, Aarhus University, 8000 Aarhus C, Denmark.

Abiotic stressors, such as cold exposure, can depolarize insect cells substantially causing cold coma and cell death. During cold exposure, insect skeletal muscle depolarization occurs through a 2-stage process. Firstly, short-term cold exposure reduces the activity of electrogenic ion pumps, which depolarize insect muscle markedly. Read More

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Discovery of a covalent inhibitor of heat shock protein 90 with antitumor activity that blocks the co-chaperone binding via C-terminal modification.

Cell Chem Biol 2021 Apr 26. Epub 2021 Apr 26.

State Key Laboratory of Natural Medicines, and Jiang Su Key Laboratory of Drug Design and Optimization, China Pharmaceutical University, Nanjing 210009, China; Department of Medicinal Chemistry, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China. Electronic address:

Heat shock protein (Hsp90), a critical molecular chaperone that regulates the maturation of a large number of oncogenic client proteins, plays an essential role in the growth of neoplastic cells. Herein, DDO-6600 is identified to covalent modification of Cys598 on Hsp90 from in silico study and is verified by a series of biological assays. We demonstrated that DDO-6600 covalently bound to Cys598 on the Hsp90 C terminus and exhibited antiproliferative activities against multiple tumor cells without inhibiting ATPase activity. Read More

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Dynamic Nuclear Structure Emerges from Chromatin Cross-Links and Motors.

Phys Rev Lett 2021 Apr;126(15):158101

Department of Physics and BioInspired Syracuse, Syracuse University, Syracuse, New York 13244, USA.

The cell nucleus houses the chromosomes, which are linked to a soft shell of lamin protein filaments. Experiments indicate that correlated chromosome dynamics and nuclear shape fluctuations arise from motor activity. To identify the physical mechanisms, we develop a model of an active, cross-linked Rouse chain bound to a polymeric shell. Read More

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Computer-aided search for 5-arylideneimidazolone anticancer agents able to overcome ABCB1-based multidrug resistance.

ChemMedChem 2021 Apr 30. Epub 2021 Apr 30.

Uniwersytet Jagiellonski w Krakowie Collegium Medicum, Dept. of Technology and Biotechnology of Drugs, ul. Medyczna 9, Not Available, 30-688, Cracow, POLAND.

ABCB1 modulation is an interesting strategy in search for new anticancer agents overcoming multidrug resistance (MDR). Hence,  17 new 5-arylideneimidazolones containing amine moiety, as potential ABCB1 inhibitors was designed, synthesized, and investigated. The series was tested in both, parental (PAR) and multidrug resistant (MDR) overproducing ABCB1, T-lymphoma cancer cells using cytotoxicity assay. Read More

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Chronic Ouabain Prevents Na,K-ATPase Dysfunction and Targets AMPK and IL-6 in Disused Rat Soleus Muscle.

Int J Mol Sci 2021 Apr 10;22(8). Epub 2021 Apr 10.

Department of General Physiology, St. Petersburg State University, 199034 St. Petersburg, Russia.

Sustained sarcolemma depolarization due to loss of the Na,K-ATPase function is characteristic for skeletal muscle motor dysfunction. Ouabain, a specific ligand of the Na,K-ATPase, has a circulating endogenous analogue. We hypothesized that the Na,K-ATPase targeted by the elevated level of circulating ouabain modulates skeletal muscle electrogenesis and prevents its disuse-induced disturbances. Read More

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Thiosemicarbazide Derivatives Decrease the ATPase Activity of Topoisomerase IV, Inhibit Mycobacterial Growth, and Affect Replication in .

Int J Mol Sci 2021 Apr 9;22(8). Epub 2021 Apr 9.

Department of Molecular Microbiology, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland.

Compounds targeting bacterial topoisomerases are of interest for the development of antibacterial agents. Our previous studies culminated in the synthesis and characterization of small-molecular weight thiosemicarbazides as the initial prototypes of a novel class of gyrase and topoisomerase IV inhibitors. To expand these findings with further details on the mode of action of the most potent compounds, enzymatic studies combined with a molecular docking approach were carried out, the results of which are presented herein. Read More

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Comparative transcriptome analysis reveals key genes potentially related to organic acid and sugar accumulation in loquat.

PLoS One 2021 29;16(4):e0238873. Epub 2021 Apr 29.

College of Environmental and Biological Engineering, Putian University, Putian, China.

Organic acids and sugars are the primary components that determine the quality and flavor of loquat fruits. In the present study, major organic acids, sugar content, enzyme activities, and the expression of related genes were analyzed during fruit development in two loquat cultivars, 'JieFangZhong' (JFZ) and 'BaiLi' (BL). Our results showed that the sugar content increased during fruit development in the two cultivars; however, the organic acid content dramatically decreased in the later stages of fruit development. Read More

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Exogenous trehalose protects photosystem II by promoting cyclic electron flow under heat and drought stresses in winter wheat.

Plant Biol (Stuttg) 2021 Apr 29. Epub 2021 Apr 29.

Instruments Sharing Platform of School of Life Sciences, East China Normal University, Shanghai, 200241, China.

Drought and the rising global temperature are important factors reducing wheat production. Trehalose protects the reaction center and improves photosystem II (PS II) activity under diverse stress conditions. However, the underlying mechanism remains unknown. Read More

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Daytime Restricted Feeding Affects Day-Night Variations in Mouse Cerebellar Proteome.

Front Mol Neurosci 2021 12;14:613161. Epub 2021 Apr 12.

Institut des Neurosciences Cellulaires et Intégratives, Université de Strasbourg, Centre National de la Recherche Scientifique, Strasbourg, France.

The cerebellum harbors a circadian clock that can be shifted by scheduled mealtime and participates in behavioral anticipation of food access. Large-scale two-dimensional difference gel electrophoresis (2D-DIGE) combined with mass spectrometry was used to identify day-night variations in the cerebellar proteome of mice fed either during daytime or nighttime. Experimental conditions led to modified expression of 89 cerebellar proteins contained in 63 protein spots. Read More

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Activation of (pro)renin by (pro)renin receptor in extracellular vesicles from osteoclasts.

Sci Rep 2021 Apr 28;11(1):9214. Epub 2021 Apr 28.

Department of Orthodontics, University of Florida College of Dentistry, Gainesville, FL, 32610, USA.

The (pro)renin receptor (PRR) is a multifunctional integral membrane protein that serves as a component of the vacuolar H-ATPase (V-ATPase) and also activates (pro)renin. We recently showed that full-length PRR, found as part of a V-ATPase sub-complex, is abundant in extracellular vesicles shed by osteoclasts. Here, we tested whether these extracellular vesicles stimulate (pro)renin. Read More

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Dietary Bioactive Diindolylmethane Enhances the Therapeutic Efficacy of Centchroman in Breast Cancer Cells by Regulating ABCB1/P-gp Efflux Transporter.

J Nutr Biochem 2021 Apr 25:108749. Epub 2021 Apr 25.

Department of Biochemistry, CSIR-Central Food Technological Research Institute, Mysore, India-570020; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad- 201002, India. Electronic address:

Overexpression of drug efflux transporters is commonly associated with multidrug-resistance in cancer therapy. Here for the first time, we investigated the ability of diindolylmethane (DIM), a dietary bioactive rich in cruciferous vegetables, in enhancing the efficacy of Centchroman (CC) by modulating the drug efflux transporters in human breast cancer cells. CC is a selective estrogen receptor modulator, having promising therapeutic efficacy against breast cancer. Read More

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Gradual opening of Smc arms in prokaryotic condensin.

Cell Rep 2021 Apr;35(4):109051

Department of Fundamental Microbiology, University of Lausanne, 1015 Lausanne, Switzerland. Electronic address:

Multi-subunit SMC ATPases control chromosome superstructure apparently by catalyzing a DNA-loop-extrusion reaction. SMC proteins harbor an ABC-type ATPase "head" and a "hinge" dimerization domain connected by a coiled coil "arm." Two arms in a SMC dimer can co-align, thereby forming a rod-shaped particle. Read More

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