38 results match your criteria γ-gcs mrna


AQP8 participates in oestrogen-mediated buffalo follicular development by regulating apoptosis of granulosa cells.

Reprod Domest Anim 2021 May 9;56(5):812-820. Epub 2021 Mar 9.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, China.

Aquaporins (AQPs), a family of small membrane-spanning proteins, are involved in fluid transport, cell signalling and reproduction. Regulating AQP8 expression influences apoptosis of granulosa cells (GCs), ovarian folliculogenesis, oogenesis and early embryonic development in mice, but its role has never been investigated in other species. The aim of the present study was to characterize the AQP8 function in buffalo follicular development. Read More

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Altered m A modification is involved in up-regulated expression of FOXO3 in luteinized granulosa cells of non-obese polycystic ovary syndrome patients.

J Cell Mol Med 2020 10 1;24(20):11874-11882. Epub 2020 Sep 1.

Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

The pathophysiology of polycystic ovary syndrome (PCOS) is characterized by granulosa cell (GC) dysfunction. m A modification affects GC function in patients with premature ovarian insufficiency (POI), but the role of m A modification in PCOS is unknown. The purpose of the prospective comparative study was to analyse the m A profile of the luteinized GCs from normovulatory women and non-obese PCOS patients following controlled ovarian hyperstimulation. Read More

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October 2020

Ethyl 2,4-dicarboethoxy pantothenate, a derivative of pantothenic acid, prevents cellular damage initiated by environmental pollutants through Nrf2 activation.

J Dermatol Sci 2018 Nov 9;92(2):162-171. Epub 2018 Sep 9.

School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakuracho, Hachiouji, Tokyo 192-0982, Japan. Electronic address:

Background: Recently, environmental pollutants have become a concern not only for respiratory organs but also for skin-related human health, because skin is localized at the border between the human body and the external environment and is easily influenced by environmental pollutants.

Objective: Here, we investigated the effects of a novel pantothenic acid (PA) derivative, ethyl 2,4-dicarboethoxy pantothenate (EDCEP), on a diesel particulate extract (DPE) as a representative environmental pollutant that generates reactive oxygen species (ROS) through the activation of aryl hydrocarbon receptor (AHR) signaling.

Methods: We characterized the effects of PA and EDCEP on normal human epidermal keratinocytes (NHEKs) exposed to DPE or HO as a general oxidative stress stimulator. Read More

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November 2018

Activation of Nrf2 might reduce oxidative stress in human granulosa cells.

Mol Cell Endocrinol 2018 07 4;470:96-104. Epub 2017 Oct 4.

Department of Obstetrics and Gynecology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113 8655, Japan.

Nuclear factor-E2-related factor 2 (Nrf2)/Kelch-like ECH-associated protein 1 (Keap1)-antioxidant response element (ARE) signaling pathway is one of the most important defense mechanisms against oxidative stress (OS). It is well documented that equilibration status of OS plays fundamental roles in human reproductive medicine, and the physiological role of Nrf2 in ovarian granulosa cells (GCs) has not been determined yet. Herein we aimed to study the function of Nrf2 in GCs. Read More

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Doxorubicin inhibits muscle inflammation after eccentric exercise.

J Cachexia Sarcopenia Muscle 2017 Apr 10;8(2):277-284. Epub 2016 Oct 10.

Laboratory of Exercise Biochemistry, University of Taipei, Taipei, Taiwan.

Background: Doxorubicin, a widely used anti-tumour drug, is known to cause muscle loss in cancer patients.

Methods: Following an acute dose of doxorubicin injection (2.5 mg/kg per body weight), we examined macrophage distribution in rat soleus muscle challenged by eccentric exercise (downhill running). Read More

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Association of polymorphisms at the microRNA binding site of the caprine KITLG 3'-UTR with litter size.

Sci Rep 2016 05 11;6:25691. Epub 2016 May 11.

College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.

This study identified three novel single nucleotide polymorphisms (SNPs) (c.1389C > T, c.1457A > C and c. Read More

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3-O-Laurylglyceryl ascorbate activates the intracellular antioxidant system through the contribution of PPAR-γ and Nrf2.

J Dermatol Sci 2016 Jun 3;82(3):189-96. Epub 2016 Mar 3.

Tokyo University of Technology, 1404-1, Katakuracho, Hachiouji, Tokyo 192-0982, Japan.

Background: Ascorbic acid (AsA) has multifunctional effects on physiology and aging including the prevention and improvement of skin pigmentation and wrinkles. AsA has scavenging effects against various types of reactive oxygen species (ROS), which are initiators of aging and premature aging of the skin. However, AsA not only has a quite unstable characteristic, but also has low skin penetration. Read More

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Autophagy protects meniscal cells from glucocorticoids-induced apoptosis via inositol trisphosphate receptor signaling.

Apoptosis 2015 Sep;20(9):1176-86

Department of Orthopedic Surgery, Xinhua Hospital, 1665 Kongjiang Road, 200092, Shanghai, China.

Intra-articular injection of glucocorticoids (GCs) has been widely used in the management of osteoarthritis and rheumatoid arthritis. Nevertheless, several studies showed that GCs had toxic effects on chondrocytes as well as synovial cells. Previously we reported the protective role of autophagy in the degeneration of meniscal tissues. Read More

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September 2015

Activation of the kynurenine pathway and increased production of the excitotoxin quinolinic acid following traumatic brain injury in humans.

J Neuroinflammation 2015 May 30;12:110. Epub 2015 May 30.

Australian New Zealand Intensive Care Research Centre, Department of Epidemiology and Preventive Medicine, Monash University, Melbourne, Australia.

Unlabelled: During inflammation, the kynurenine pathway (KP) metabolises the essential amino acid tryptophan (TRP) potentially contributing to excitotoxicity via the release of quinolinic acid (QUIN) and 3-hydroxykynurenine (3HK). Despite the importance of excitotoxicity in the development of secondary brain damage, investigations on the KP in TBI are scarce. In this study, we comprehensively characterised changes in KP activation by measuring numerous metabolites in cerebrospinal fluid (CSF) from TBI patients and assessing the expression of key KP enzymes in brain tissue from TBI victims. Read More

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An increase in glucosylceramide synthase induces Bcl-xL-mediated cell survival in vinorelbine-resistant lung adenocarcinoma cells.

Oncotarget 2015 Aug;6(24):20513-24

Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.

Reversing drug resistance with concurrent treatment confers anticancer benefits. In this study, we investigated the potential mechanism of glucosylceramide synthase (GCS)-mediated vinca alkaloid vinorelbine (VNR) resistance in human lung adenocarcinoma cells. Compared with PC14PE6/AS2 (AS2) and CL1-0 cells, apoptotic analysis showed that both A549 and CL1-5 cells were VNR-resistant, while these cells highly expressed GCS at the protein level. Read More

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The NR3C1 Glucocorticoid Receptor Gene Polymorphisms May Modulate the TGF-beta mRNA Expression in Asthma Patients.

Inflammation 2015 Aug;38(4):1479-92

Department of Internal Medicine, Asthma and Allergy, Medical University of Lodz, 22 Kopcinskiego Str, 90-153, Lodz, Poland,

Glucocorticosteroids (GCs) are basic drugs in therapy of a number of diseases, including chronic diseases of the respiratory system. They are the most important anti-inflammatory drugs in the treatment of asthma. GCs after binding to the glucocorticoid receptor (GR) form the complex (transcription factor), which acts on promoter and regulatory parts of genes enhancing the expression of anti-inflammatory proteins and decreasing the proinflammatory protein synthesis, including numerous cytokines mediating inflammation in the course of asthma. Read More

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Methoxychlor and its metabolite HPTE inhibit cAMP production and expression of estrogen receptors α and β in the rat granulosa cell in vitro.

Reprod Toxicol 2015 Jan 27;51:72-8. Epub 2014 Dec 27.

Department of Animal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA; Joint Graduate Program in Toxicology, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA. Electronic address:

The major metabolite of the estrogenic pesticide methoxychlor (MXC) HPTE is a stronger ESR1 agonist than MXC and acts also as an ESR2 antagonist. In granulosa cells (GCs), FSH stimulates estradiol via the second messenger cAMP. HPTE inhibits estradiol biosynthesis, and this effect is greater in FSH-treated GCs than in cAMP-treated GCs. Read More

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January 2015

[Dopaminergic neuroprotection via Nrf2-ARE pathway activation: identification of an activator from green perilla leaves].

Authors:
Yasuhiko Izumi

Yakugaku Zasshi 2013 ;133(9):983-8

Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan.

Parkinson disease is one of the most common neurodegenerative disorders and is characterized by the selective loss of dopaminergic neurons in the substantia nigra. Although a decrease in proteasome activity has been found in patients with sporadic Parkinson disease, the relationship between the ubiquitin-proteasome system and dopaminergic neuronal death remains to be elucidated. Here, we review a mechanism in which proteasome inhibition provides dopaminergic neuroprotection from oxidative stress. Read More

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11β-Hydroxysteroid dehydrogenase type 1 selective inhibitor BVT.2733 protects osteoblasts against endogenous glucocorticoid induced dysfunction.

Endocr J 2013 12;60(9):1047-58. Epub 2013 Jun 12.

Department of Geratology, the First Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu Province, People's Republic of China.

Pharmacologic glucocorticoids (GCs) inhibit osteoblast function and induce osteoporosis. 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) may play a role in osteoporosis as it regulates GC action at a pre-receptor level by converting inactive GC to its active form. Further, 11β-HSD1 was found increasingly expressed in bone with age. Read More

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Characterization of white shrimp Litopenaeus vannamei integrin β and its role in immunomodulation by dsRNA-mediated gene silencing.

Dev Comp Immunol 2013 Jun 31;40(2):167-79. Epub 2013 Jan 31.

Department of Aquaculture, National Taiwan Ocean University, Keelung 202, Taiwan, ROC.

The full sequence of white shrimp Litopenaeus vannamei integrin β (LV-B) is 2879bp which encodes 787 amino acids (aa) of the open reading frame (ORF). The mature protein (764 aa) contains (1) an extracellular domain (ED) of 692 aa, (2) a transmembrane domain (TD) of 23 aa, and (3) a cytoplasmic domain (CD) of 49 aa. The cloned LV-B grouped together with crayfish Pacifastacus leniusculus integrin β (PL-B1), but was far away from vertebrate integrin β1, β3, β5, β6, β7, and β8, and another L. Read More

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Therapeutic potential of EGCG on acute renal damage in a rat model of obstructive nephropathy.

Mol Med Rep 2013 Apr 28;7(4):1096-102. Epub 2013 Jan 28.

Department of Pediatrics, Shengjing Hospital Of China Medical University, Shenyang 110004, P.R. China.

As a major active component in green tea, (-)-epigallocatechin 3-O-gallate (EGCG) has many anti-oxidative activities. This study investigated whether intraperitoneal administration of EGCG was capable of suppressing oxidative stress in rats with unilateral ureteral obstruction (UUO) and probed the potential mechanisms involved. In total, 150 adult male rats were randomly divided into 5 groups (n=30 each): the control group (group N); the unilateral ureteral obstruction (UUO) group (group C), where the unilateral ureter was ligated resulting in an obstructive nephropathy model; and the EGCG group (group T), following unilateral ureteral ligation, rats were intraperitoneally injected with EGCG at a dosage of 2. Read More

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Glucocorticoid resistance in chronic lymphocytic leukaemia is associated with a failure of upregulated Bim/Bcl-2 complexes to activate Bax and Bak.

Cell Death Dis 2012 Aug 16;3:e372. Epub 2012 Aug 16.

Department of Molecular and Clinical Cancer Medicine, University of Liverpool, Liverpool, UK.

Glucocorticoids (GCs) represent an important component of modern treatment regimens for fludarabine-refractory or TP53-defective chronic lymphocytic leukemia (CLL). However, GC therapy is not effective in all patients. The molecular mechanisms responsible for GC-induced apoptosis and resistance were therefore investigated in primary malignant cells obtained from a cohort of 46 patients with CLL. Read More

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Downregulation of glutamine synthetase via GLAST suppression induces retinal axonal swelling in a rat ex vivo hydrostatic pressure model.

Invest Ophthalmol Vis Sci 2011 Aug 22;52(9):6604-16. Epub 2011 Aug 22.

Department of Ophthalmology, Akita University School of Medicine, Japan.

PURPOSE. High levels of glutamate can be toxic to retinal GCs. Thus, effective buffering of extracellular glutamate is important in preserving retinal structure and function. Read More

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Glucocorticoid suppresses steroidogenesis in rat progenitor Leydig cells.

J Androl 2010 Jul-Aug;31(4):365-71. Epub 2010 Jan 14.

Population Council, New York, NY 10065, USA.

Glucocorticoid (GC) inhibits testosterone production in adult Leydig cells by the glucocorticoid receptor (GR). However, whether GC affects the development of Leydig cells is unclear. The goal of the present study is to investigate the effects of GC on steroidogenesis of rat progenitor Leydig cells (PLCs) in vitro. Read More

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January 2011

Molecular changes to HeLa cells on continuous exposure to SN-38, an active metabolite of irinotecan hydrochloride.

Cancer Lett 2009 Jun 6;278(1):88-96. Epub 2009 Feb 6.

Department of Clinical Pharmacy, Division of Clinical Pharmaceutical Sciences, Kyoto Pharmaceutical University, 5 Nakauchi-cho, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan.

It is important to clarify the molecular characteristics of tumor cells showing multidrug resistance (MDR) and to identify the novel targets or biomarkers for chemotherapy. The aim of this study is to establish resistant HeLa sublines through exposure to SN-38, an active metabolite of irinotecan hydrochloride, and to investigate their molecular changes. HeLa cells were exposed to SN-38 at 1, 10, or 100 nM, and resistant clones were isolated and named HeLa/SN1, HeLa/SN10, and HeLa/SN100, respectively. Read More

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Effects of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf.) hull extracts on the secretion of progesterone and estradiol in vivo and in vitro.

Exp Biol Med (Maywood) 2007 Oct;232(9):1181-94

Graduate Institute of Food Science and Technology, Center for Food and Biomolecules, College of Bioresources and Agriculture, National Taiwan University, Taipei 106, Taiwan, Republic of China.

Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf. Read More

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October 2007

Antigen-specific immunoglobulin E+ B cells are preferentially localized within germinal centres.

Immunology 2007 Mar 1;120(3):345-53. Epub 2006 Dec 1.

Department of Pathology and Laboratory Medicine, Geffen School of Medicine at University of California Los Angeles, Los Angeles, CA 90095, USA.

Allergen-specific immunoglobulin E (IgE) mediates immediate-type hypersensitivity reactions and plays a central role in allergic diseases. Although antigen-driven B-cell maturation and isotype switching occur within germinal centres (GCs), the role of GCs in IgE production is poorly understood. In view of this, we investigated the development of IgE-expressing cells within GCs in response to an extensively characterized antigen, 2-phenyloxazolone (phOx). Read More

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Role of the proteasome in TGF-beta signaling in lens epithelial cells.

Invest Ophthalmol Vis Sci 2006 May;47(5):2045-52

Department of Biochemistry and Molecular Biology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark 07101-1709, USA.

Purpose: The durability of the ubiquitin proteasome pathway in the mammalian lens makes this enzyme system a potential contributor to certain cataracts and posterior capsular opacification (PCO). The present study addresses proteasome involvement in TGF-beta induced, cataract-associated gene activation in human lens cells.

Methods: HLE B-3 cells were treated with TGF-beta, in combination with the proteasome inhibitors MG-132 or lactacystin. Read More

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Thyroid hormone, but not parathyroid hormone, partially restores glucocorticoid-induced growth retardation.

Pediatr Nephrol 2005 Mar 2;20(3):335-41. Epub 2005 Feb 2.

Department of Metabolic and Endocrine Diseases, University Medical Center Utrecht, P.O. Box 85090, 3508 AB, Utrecht, The Netherlands.

Growth retardation is a serious side effect of long-term glucocorticoid (GC) treatment. In order to prevent or diminish this deleterious effect, a combination therapy including growth hormone (GH), a stimulator of bone growth, is often recommended. Parathyroid hormone (PTH) and thyroid hormone (T(4)) are important hormonal regulators of bone growth, and might also be helpful anabolic agents for counteracting the negative effects of GCs. Read More

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Mechanism of cortisol/progesterone antagonism in the regulation of 15-hydroxyprostaglandin dehydrogenase activity and messenger ribonucleic acid levels in human chorion and placental trophoblast cells at term.

J Clin Endocrinol Metab 2003 Jun;88(6):2922-33

Canadian Institutes for Health Research Group in Fetal and Neonatal Health and Development, Department of Physiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8.

Prostaglandin dehydrogenase (PGDH) metabolizes prostaglandins (PGs) to render them inactive. We reported previously that cortisol (F) decreases and progesterone (P(4)) maintains PGDH activity/expression in human chorion and placenta. Furthermore, we have shown that F and P(4) compete for regulation of PGDH. Read More

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Unique action determinants of double acting topoisomerase inhibitor, TAS-103.

Int J Oncol 2001 Nov;19(5):921-7

Department of Biochemistry and Biophysics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan.

O6-methylguanine-DNA methyltransferase (MGMT), gamma-glutamylcysteine synthetase (gamma-GCS), and glutathione (GSH) are found to participate in resistance to TAS-103, a topoisomerase I/II inhibitor. In 13 human cancer cell lines, MGMT expression correlated with IC50 for TAS-103, whereas gamma-GCS expression inversely correlated with the IC50 value, suggesting MGMT may work to decrease TAS-103 activity but gamma-GCS may increase it. A reduced gamma-GCS and GSH, and an increased MGMT were associated with the development of resistance in A549 and DLD cells, and gamma-GCS inhibition by buthionine sulphoximine increased the TAS-103 resistance, whereas MGMT inhibition by both O6-benzyl-guanine and MGMT-antisense transfection sensitized cells to TAS-103. Read More

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November 2001

Cysteine regulates expression of cysteine dioxygenase and gamma-glutamylcysteine synthetase in cultured rat hepatocytes.

Am J Physiol Endocrinol Metab 2001 May;280(5):E804-15

Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA.

Rat hepatocytes cultured for 3 days in basal medium expressed low levels of cysteine dioxygenase (CDO) and high levels of gamma-glutamylcysteine synthetase (GCS). When the medium was supplemented with 2 mmol/l methionine or cysteine, CDO activity and CDO protein increased by >10-fold and CDO mRNA increased by 1.5- or 3. Read More

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Human placenta sphingomyelinase, an exogenous acidic pH-optimum sphingomyelinase, induces oxidative stress, glutathione depletion, and apoptosis in rat hepatocytes.

Hepatology 2000 Jul;32(1):56-65

Liver Unit, Instituto Malalties Digestives, Hospital Clinic i Provencial, Instituto de Investigaciones Biom¿edicas, August Pi i Sunyer, Consejo Superior Investigaciones Cient¿ificas, Barcelona 08036, Barcelona, Spain.

Ceramide has been identified as a putative lipid messenger that mediates diverse cellular processes including cell death. Since glutathione (GSH) depletion is known to sensitize cells to many cytotoxic agents and as a result of the reported regulation of neutral sphyngomyelinase (NSMase) by GSH, the present study compared the role of individual SMases in the induction of oxidative stress, regulation of cellular GSH, and apoptosis of rat hepatocytes. Exposure of cultured rat hepatocytes to exogenous Bacillus cereus sphingomyelinase (bSMase), a neutral SMase, or human placenta sphingomyelinase (hSMase), an acidic SMase (ASMase), generated similar ceramide levels in a dose-dependent manner. Read More

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Effect of thioacetamide on the hepatic expression of gamma-glutamylcysteine synthetase subunits in the Rat.

Toxicol Appl Pharmacol 1999 Sep;159(3):161-8

USC Liver Disease Research Center, USC School of Medicine, Los Angeles, California, 90033, USA.

Glutathione (GSH) is the main nonprotein thiol important in antioxidant defense and maintenance of the intracellular redox state. A major determinant of the rate of GSH synthesis is the activity of the rate-limiting enzyme, gamma-glutamylcysteine synthetase (GCS). A heavy (HS) and light subunit (LS) make up GCS; oxidative stress regulates both transcriptionally. Read More

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September 1999

Effects of nonsulfur and sulfur amino acids on the regulation of hepatic enzymes of cysteine metabolism.

Am J Physiol 1999 07;277(1):E144-53

Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA.

To determine the role of nonsulfur vs. sulfur amino acids in regulation of cysteine metabolism, rats were fed a basal diet or diets supplemented with a mixture of nonsulfur amino acids (AA), sulfur amino acids (SAA), or both for 3 wk. Hepatic cysteine-sulfinate decarboxylase (CSDC), cysteine dioxygenase (CDO), and gamma-glutamylcysteine synthetase (GCS) activity, concentration, and mRNA abundance were measured. Read More

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