Hepatology 2000 Jul;32(1):56-65
Liver Unit, Instituto Malalties Digestives, Hospital Clinic i Provencial, Instituto de Investigaciones Biom¿edicas, August Pi i Sunyer, Consejo Superior Investigaciones Cient¿ificas, Barcelona 08036, Barcelona, Spain.
Ceramide has been identified as a putative lipid messenger that mediates diverse cellular processes including cell death. Since glutathione (GSH) depletion is known to sensitize cells to many cytotoxic agents and as a result of the reported regulation of neutral sphyngomyelinase (NSMase) by GSH, the present study compared the role of individual SMases in the induction of oxidative stress, regulation of cellular GSH, and apoptosis of rat hepatocytes. Exposure of cultured rat hepatocytes to exogenous Bacillus cereus sphingomyelinase (bSMase), a neutral SMase, or human placenta sphingomyelinase (hSMase), an acidic SMase (ASMase), generated similar ceramide levels in a dose-dependent manner. Read More